CN1847390A - Composite protectant for freeze dried lactobcillus fermenting prepn - Google Patents
Composite protectant for freeze dried lactobcillus fermenting prepn Download PDFInfo
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- CN1847390A CN1847390A CN 200610012688 CN200610012688A CN1847390A CN 1847390 A CN1847390 A CN 1847390A CN 200610012688 CN200610012688 CN 200610012688 CN 200610012688 A CN200610012688 A CN 200610012688A CN 1847390 A CN1847390 A CN 1847390A
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- freeze dried
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- lactobcillus
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Abstract
The present invention discloses one kind of composite protectant for freeze dried lactobacillus fermenting preparation. The composite protectant for freeze dried lactobacillus fermenting preparation consists of skim milk powder, cane sugar, mycose, Tween-800, yeast extract, blood serum, vitamin E and water in certain weight proportion. The freeze dried lactobacillus fermenting preparation with the composite protectant has live lactobacillus content over 1.0*10<>11</>cfu/g and preservation period after normal pressure sealing and at 4 deg.c of 1 year, and then, it may be inoculated to milk in the inoculation amount below 100 ppm to form milk curd in 4 hr. The freeze dried lactobacillus fermenting preparation with the composite protectant may be used as directly thrown leaven for producing sour milk and fermented sour milk beverage.
Description
Technical field
The present invention relates to a kind of composite protectant of freeze dried lactobcillus fermenting preparation, belong to lactobacillus product production
Technical field.
Background technology
Along with human living standard's the raising and the enhancing of public health consciousness, China's cultured milk prod industrial development is swift and violent, and wherein, the development of sour milk and leben industry occupies first of the cultured milk prod industry.The quality product of the quality of starter and sour milk and leben is closely related.The preparation of the artificial type starter of tradition generally need be passed through technological processs such as actication of culture, mother starter, middle starter, productive leavening agent, have that viable bacteria content is low, inoculum size is big, preservation term is short, production process is many, labour intensity is big, the cycle is long, bacterial classification is very easily degenerated and drawback such as pollution, directly cause sour milk and leben quality product unstable and production efficiency is low.Efficient concentrated type direct-throwing lactobacillus starter be lactobacillus inoculation through the deep liquid high-density culture, concentrating and separating, miscible with the protective material medium, through freezing or lyophilize, sterile packed forms again.Its viable bacteria content height, preservation term is long, can directly buy as productive leavening agent to professional production producer and use, and inoculum size is lower, and operation is simplified, and saves investment and space, has prevented the degeneration and the pollution of bacterial classification, and labour productivity and quality product improve greatly.Concentrated type cold storage starter is in production application, and it is higher to keep freezing expense, and transportation is inconvenient, needs to be equipped with special-purpose refrigerator car, and is strong excessively to the dependency at starter center.And the concentrated type freeze dried fermenting preparation, preservation and convenient transportation, the mechanization degree height is convenient to industrialized mass production, is the main developing direction of concentrated type lactobacillus starter from now on.In the world, begin to develop the vacuum lyophilization starter, formed certain scale of commercially producing at present as far back as phase early 1970s.The research of China is started late, and at present, domesticly commercially produces as yet.The starter technology of preparing of most sour milks and leben enterprise still rests on the level of the artificial type starter of tradition; Though the concentrated type lactobacillus starter excellent quality of import, cost is higher, 1 ton of fermented-milk of average every production, and production cost increases about 70 yuan.Therefore, make full use of Chinese resources advantage, it is imperative actively to develop and develop the efficient concentrated type direct-throwing lactobacillus starter with China's independent intellectual property right.The principal element that influences efficient concentrated type freeze dried lactobcillus fermenting preparation cell survival rate and storage stability has: strain characteristic, substratum, culture condition, harvesting time, initiator cell concentration, lyophilized vaccine and pH value thereof, chilling rate, drying process, residual moisture, packaged form, preservation temperature etc.Wherein, lyophilized vaccine is the most complicated, the key factor of difficult selection; it not only influences the cell survival rate of milk-acid bacteria in freeze-drying process; also influence the cell stability between preservation term; so; seek rational lyophilized vaccine, significant for the development that promotes efficient concentrated type direct-throwing lactobacillus starter.
Summary of the invention
The composite protectant that the purpose of this invention is to provide a kind of freeze dried lactobcillus fermenting preparation has improved cell survival rate and storage stability between concentrated type freeze dried lactobcillus fermenting preparation preservation term.
Design of the present invention is such.The inventor is so that the lactobacillus bulgaricus L.b-S1 and the thermophilus streptococcus S.t-3 of isolation and selection are test strain voluntarily; at first studied the influence of 29 kinds of single protective materials of food grade to lactic-acid bacteria cells freeze-drying survival rate, preliminary screening goes out to resist the single protective material of freezing or resist drying.On this basis, studied the influence of different composite protectants to lactic-acid bacteria cells freeze-drying survival rate, screening and optimizing goes out 6 kinds of good anti-cryodesiccated complex protection agent prescriptions.Then, the freeze dried lactobcillus fermenting preparation made from 6 kinds of composite protectants is respectively carried out the cell accelerated test, further filter out a kind of not only anti-lyophilize but also long keeping good composite protectant.The composite protectant of the said freeze dried lactobcillus fermenting preparation of the present invention consists of: skimmed milk powder 10g, sucrose 3～7g, trehalose 0.5～1.5g, tween 80 0.05～0.15g, yeast extract paste 0.5～1.5g, serum 0.5～1.5g, vitamin-E 0.05～0.15g, water 100mL.
The composite protectant preparation process of freeze dried lactobcillus fermenting preparation of the present invention is as follows:
1. will form the accurately weighing of each component (except serum, the vitamin-E) of this composite protectant, mix;
2. heating for dissolving is cooled to room temperature;
3. reconcile about pH value to 7.0 with NaOH solution;
4. 112 ℃, the 10min sterilization is cooled to room temperature;
5. adopt aseptic technique to add the serum and the vitamin-E of filtration sterilization, mixing can make finished product.
The present invention has obtained following technique effect: the freeze dried lactobcillus fermenting preparation that will utilize composite protectant of the present invention to make is packed in the difference mode of sealing up for safekeeping; under differing temps, preserve; regularly detect the viable count and the leavening property of starter; the storage conditions and the preservation term of the freeze dried lactobcillus fermenting preparation that this composite protectant makes have been determined to adopt; investigated this protectant practical application effect: adopt lactobacillus bulgaricus L.b-S1 that this composite protectant makes and single bacterial strain or the hybrid bacterial strain freeze dried fermenting preparation of thermophilus streptococcus S.t-3, its viable bacteria content all reaches 1.0 * 10
10More than the cfu/g; The lactobacillus bulgaricus L.b-S1 and the thermophilus streptococcus S.t-3 hybrid bacterial strain freeze dried fermenting preparation that utilize this composite protectant to make, carry out 42 ℃ of Yoghourt fermentations with ten thousand/following inoculum size, 4h gets final product curdled milk, the sour milk product of its fermentation, acidity reaches about 80 ° of T, the pH value is about 4.5, and the sense organ local flavor is all good, and the more traditional artificial fermentation's agent of inoculum size reduces by 100 times～1000 times; Adopt lactobacillus bulgaricus L.b-S1 that this composite protectant makes and single bacterial strain or the hybrid bacterial strain freeze dried fermenting preparation of thermophilus streptococcus S.t-3; seal up for safekeeping under the different preservation temperature of mode or same preservation temperature difference is sealed up for safekeeping under the mode same; preservation 1 year, viable bacteria content is all 10
10More than the cfu/g, and the leavening property of freeze dried fermenting preparation between preservation term is stable, compares with initial freeze dried fermenting preparation, and no significant difference can be used as efficient concentrated type throw type leaven and uses.
Embodiment
Following examples are used to illustrate the present invention.
Embodiment 1
One, the composite protectant of freeze dried lactobcillus fermenting preparation is formed: 10g skimmed milk powder, 5g sucrose, 1g trehalose, 0.1g tween 80,1g yeast extract paste, 1g serum, 0.1g vitamin-E, water 100mL.
Two, preparation:
With the accurate weighing of each component of this composite protectant of composition except serum, the vitamin-E, mix; Heating for dissolving is cooled to room temperature; Reconcile about pH value to 7.0 with NaOH solution; 112 ℃, the 10min sterilization is cooled to room temperature; Adopt aseptic technique to add the serum and the vitamin-E of filtration sterilization, mixing gets product.The freeze-dried vaccine powder viable count of lactobacillus bulgaricus L.b-S1 reaches 7.34 * 10
10Cfu/g.
Embodiment 2
One, the composite protectant of freeze dried lactobcillus fermenting preparation is formed: 10g skimmed milk powder, 3g sucrose, 0.5g trehalose, 0.05g tween 80,0.5g yeast extract paste, 1.5g serum, 0.05g vitamin-E, water 100mL.
Two, preparation:
With the accurate weighing of each component of this composite protectant of composition except serum, the vitamin-E, mix; Heating for dissolving is cooled to room temperature; Reconcile about pH value to 7.0 with NaOH solution; 112 ℃, the 10min sterilization is cooled to room temperature; Adopt aseptic technique to add the serum and the vitamin-E of filtration sterilization, mixing gets product.The freeze-dried vaccine powder viable count of lactobacillus bulgaricus L.b-S1 reaches 8.98 * 10
10Cfu/g.
Embodiment 3
One, the composite protectant of freeze dried lactobcillus fermenting preparation consists of: 10g skimmed milk powder, 5g sucrose, 1g trehalose, 0.05g tween 80,1g yeast extract paste, 1g serum, 0.05g vitamin-E, water 100mL.
Two, preparation:
With the accurate weighing of each component of this composite protectant of composition except serum, the vitamin-E, mix; Heating for dissolving is cooled to room temperature; Reconcile about pH value to 7.0 with NaOH solution; 112 ℃, the 10min sterilization is cooled to room temperature; Adopt aseptic technique to add the serum and the vitamin-E of filtration sterilization, mixing gets product.The freeze-dried vaccine powder viable count of lactobacillus bulgaricus L.b-S1 reaches 5.89 * 10
10Cfu/g.
Embodiment 4
One, the composite protectant of freeze dried lactobcillus fermenting preparation is formed: 10g skimmed milk powder, 7g sucrose, 1g trehalose, 0.05g tween 80,0.5g yeast extract paste, 0.5g serum, 0.15g vitamin-E, water 100mL.
Two, preparation:
With the accurate weighing of each component of this composite protectant of composition except serum, the vitamin-E, mix; Heating for dissolving is cooled to room temperature; Reconcile about pH value to 7.0 with NaOH solution; 112 ℃, the 10min sterilization is cooled to room temperature; Adopt aseptic technique to add the serum and the vitamin-E of filtration sterilization, mixing gets product.The freeze-dried vaccine powder viable count of thermophilus streptococcus S.t-3 reaches 2.71 * 10
10Cfu/g.
Embodiment 5
One, the composite protectant of freeze dried lactobcillus fermenting preparation is formed: 10g skimmed milk powder, 3g sucrose, 1.5g trehalose, 0.15g tween 80,1.5g yeast extract paste, 1g serum, 0.05g vitamin-E, water 100mL.
Two, preparation:
With the accurate weighing of each component of this composite protectant of composition except serum, the vitamin-E, mix; Heating for dissolving is cooled to room temperature; Reconcile about pH value to 7.0 with NaOH solution; 112 ℃, the 10min sterilization is cooled to room temperature; Adopt aseptic technique to add the serum and the vitamin-E of filtration sterilization, mixing gets product.The freeze-dried vaccine powder viable count of thermophilus streptococcus S.t-3 reaches 6.23 * 10
10Cfu/g.
Claims (6)
1, a kind of composite protectant of freeze dried lactobcillus fermenting preparation is characterized in that consisting of: skimmed milk powder 10g, sucrose 3～7g, trehalose 0.5～1.5g, tween 80 0.05～0.15g, yeast extract paste 0.5～1.5g, serum 0.5～1.5g, vitamin-E 0.05～0.15g, water 100mL.
2, by the composite protectant of the described freeze dried lactobcillus fermenting preparation of claim 1, it is characterized in that consisting of: skimmed milk powder 10g, sucrose 5g, trehalose 1g, tween 80 0.1g, yeast extract paste 1g, serum 1g, vitamin-E 0.1g, water 100mL.
3, by the composite protectant of the described freeze dried lactobcillus fermenting preparation of claim 1, it is characterized in that consisting of: skimmed milk powder 10g, sucrose 3g, trehalose 0.5g, tween 80 0.05g, yeast extract paste 0.5g, serum 1.5g, vitamin-E 0.05g, water 100mL.
4, by the composite protectant of the described freeze dried lactobcillus fermenting preparation of claim 1, it is characterized in that consisting of: skimmed milk powder 10g, sucrose 5g, trehalose 1g, tween 80 0.05g, yeast extract paste 1g, serum 1g, vitamin-E 0.05g, water 100mL.
5, by the composite protectant of the described freeze dried lactobcillus fermenting preparation of claim 1, it is characterized in that consisting of: skimmed milk powder 10g, sucrose 7g, trehalose 1g, tween 80 0.05g, yeast extract paste 0.5g, serum 0.5g, vitamin-E 0.15g, water 100mL.
6, by the composite protectant of the described freeze dried lactobcillus fermenting preparation of claim 1, it is characterized in that consisting of: skimmed milk powder 10g, sucrose 3g, trehalose 1.5g, tween 80 0.15g, yeast extract paste 1.5g, serum 1g, vitamin-E 0.05g, water 100mL.
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| CNB2006100126880A CN100404665C (en) | 2006-05-12 | 2006-05-12 | Composite protectant for freeze dried lactobcillus fermenting prepn |
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| CNB2006100126880A CN100404665C (en) | 2006-05-12 | 2006-05-12 | Composite protectant for freeze dried lactobcillus fermenting prepn |
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| CN100404665C CN100404665C (en) | 2008-07-23 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869138A (en) * | 2010-05-28 | 2010-10-27 | 江苏省农业科学院 | High-efficiency protection liquid for storage of microcapsule leaven |
| CN103141722A (en) * | 2013-03-13 | 2013-06-12 | 河北农业大学 | Preparation method of lactobacillus rhamnosus soybean milk direct vat set starter |
| CN101606552B (en) * | 2009-05-06 | 2013-09-11 | 任发政 | Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948783B (en) * | 2010-08-31 | 2012-03-21 | 广州广牧丰生物技术有限公司 | Culture medium capable of improving viable count of lactic acid bacillus |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077223A (en) * | 1992-04-01 | 1993-10-13 | 赵克强 | Life-preserving method of live-lactobacillus |
| US5840318A (en) * | 1993-05-11 | 1998-11-24 | Immunom Technologies, Inc. | Methods and compositions for modulating immune systems of animals |
| CN1623408A (en) * | 2003-12-02 | 2005-06-08 | 北京三元食品股份有限公司 | Optimized lactic acid bacteria and life beneficial bacteria frozen protective agent composition |
| CN1259413C (en) * | 2004-02-26 | 2006-06-14 | 天津科技大学 | Preparation process of fermenting agent for beneficial growing lactic bacteria and application in fresh cheese thereof |
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2006
- 2006-05-12 CN CNB2006100126880A patent/CN100404665C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606552B (en) * | 2009-05-06 | 2013-09-11 | 任发政 | Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof |
| CN101869138A (en) * | 2010-05-28 | 2010-10-27 | 江苏省农业科学院 | High-efficiency protection liquid for storage of microcapsule leaven |
| CN101869138B (en) * | 2010-05-28 | 2012-05-30 | 江苏省农业科学院 | High-efficiency protection liquid for storage of microcapsule leaven |
| CN103141722A (en) * | 2013-03-13 | 2013-06-12 | 河北农业大学 | Preparation method of lactobacillus rhamnosus soybean milk direct vat set starter |
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| CN100404665C (en) | 2008-07-23 |
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Assignee: Deyi Milk Industry Co., Ltd., Shandong Assignor: Agricultural University of Hebei Contract record no.: 2010370000469 Denomination of invention: Composite protectant for freeze dried lactobcillus fermenting prepn Granted publication date: 20080723 License type: Exclusive License Open date: 20061018 Record date: 20100820 |
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Granted publication date: 20080723 Termination date: 20120512 |