CN101869138B - High-efficiency protection liquid for storage of microcapsule leaven - Google Patents
High-efficiency protection liquid for storage of microcapsule leaven Download PDFInfo
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- CN101869138B CN101869138B CN2010101857150A CN201010185715A CN101869138B CN 101869138 B CN101869138 B CN 101869138B CN 2010101857150 A CN2010101857150 A CN 2010101857150A CN 201010185715 A CN201010185715 A CN 201010185715A CN 101869138 B CN101869138 B CN 101869138B
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Abstract
The invention relates to a formula of high-efficiency protection liquid for the storage of a microcapsule leaven, which belongs to the technical field of biological engineering. The protection liquid is prepared from the following components in the optimum formula ratio: 0.9% of sodium chloride, 3% of glucose, 5% of cane sugar, 10% of glycerol, 0.2% of calcium carbonate and the like. The invention can solve the storage problem of the microcapsule leaven and effectively prolongs the service life of the leaven.
Description
One, technical field
The present invention is a kind of high-efficiency protection liquid for storage of microcapsule leaven prescription, belongs to technical field of bioengineering.
Two, background technology
Along with the fast development of China's leavened food industry, the starter industry has been proposed new requirement, demand rises year after year.At present the most ofs large-scale diary processing of China enterprise adopts the direct putting type ferment agent for sour milk of external import, its steady quality, produces and is prone to capable and cell density can reach 10
11Cfu/g, however cost an arm and a leg, and production cost is big.Can only adopt lower-cost traditional subculture formula ferment agent for sour milk during therefore small-sized and medium-sized diary processing enterprise production sour milk, owing to reasons such as pollution in the process of going down to posterity and various bacterial classification proportional imbalances cause quality product relatively poor.Microcapsule leaven is the once innovation of starter, uses natural or the synthetic macromolecular material is wrapped in milk-acid bacteria in the minigel of semipermeability or sealing cyst membrane, and under proper condition, milk-acid bacteria discharges.Adopt microcapsulary that the milk-acid bacteria thalline is carried out embedding, strengthened the thalline resistivity of environmental factors to external world, significantly improve the survival rate of thalline behind cryopreservation phase and arrival enteron aisle, make milk-acid bacteria play wholesome effect better.The production that microcapsule leaven is used for fermented dairy prod is of far-reaching significance.In processes such as the storage of microcapsule leaven, transportation, sale, exist the thalline in the capsule to reduce gradually, capsule intensity descends even broken problem, so that reduces the starter quality, influences the food fermentation effect.How preserving the capsule starter makes thalline keep maximum viable bacteria amount to be assistant officer's problem to be solved with capsular intensity, to study at present and do not see that also the someone studies.The present invention is directed to this problem and further investigate, develop a kind of protection liquid for storage that can effectively protect the capsule starter.
Three, summary of the invention
Technical problem the objective of the invention is to develop a kind of protection liquid for storage of microcapsule leaven to the problem that the viable bacteria amount descends and capsule intensity descends in the capsule starter in storage, transportation and the sales process of capsule starter.Microcapsule leaven can effectively reduce the decline of viable bacteria amount and intensity in this protection liquid for storage, the time limit of service of prolongation starter fundamentally solves the storage problem of microcapsule leaven, is a kind of protection liquid for storage of microcapsule leaven efficiently.
Technical scheme
The present invention is a kind of protection liquid for storage of microcapsule leaven efficiently, and by sodium-chlor, glucose sugar, sucrose, glycerine and lime carbonate are formed.Its optimum formula ratio is sodium-chlor 0.9%, glucose sugar 3%, sucrose 5%, glycerine 10%, lime carbonate 0.2%.
Beneficial effect
The present invention is directed in the processes such as storage at microcapsule leaven, transportation, sale, the thalline in the capsule can reduce gradually, so that reduces the starter quality, influences the food fermentation effect.In protection liquid for storage of the present invention, can effectively protect microcapsule leaven, prolong the storage and the time limit of service of starter.Improve the international competitiveness of China's capsule starter product, promote the Sustainable development of fermented food industry, increase the contribution of food-processing industry national economy, social development.Dairy production industry is then had big meaning, can improve the scientific and technological level that China's cultured milk prod is produced, strengthen the enterprise technology original creativity, increase technology content, optimize product structure, the existing dairy production technology of improvement reduces production costs.
Major advantage of the present invention and positively effect are following:
1, microcapsule leaven can effectively reduce the decline of number of viable in the capsule and the reduction of capsule intensity, the time limit of service that has prolonged highdensity microcapsule leaven in protection liquid for storage of the present invention.
2, this storage protection liquid formula is simple, and cost is lower, and all components can safe edible.
3, the invention of this prescription can improve the international competitiveness of China's starter, and can promote the Sustainable development of fermented food industry.
Four, description of drawings
The viable count of Fig. 1 capsule starter in protection liquid for storage and sterilized water and the change curve of capsule intensity
Cell density in the ■ storage liquid; Cell density in the sterilized water; Capsule intensity in the ▲ storage liquid; Capsule intensity in the △ sterilized water
Five, embodiment
On protectant basis of using when free thalline being preserved according to forefathers; And combine the result of this problem to the capsule intensity Study on influencing factors; Selecting saline water for use is the protection liquid on basis; Adding some sugars, glycerine and lime carbonate therein, at first carry out experiment of single factor, is the basis with saline water; Add glucose (1%, 3%, 5%, 7%, 9%), sucrose (1%, 3%, 5%, 7%, 9%), glycerine (5%, 10%, 15%, 20%) and the lime carbonate (0.05%, 0.1%, 0.2%, 0.3%, 0.4%) of different content therein, study of the influence of these factors thalline activity and capsule intensity in the capsule.According to design preparation protection liquid for storage; 121 ℃ of sterilizations are after 20 minutes; The high-density microcapsule leaven for preparing is put into protection liquid for storage under 4 ℃ of conditions, preserved one month, adopt the broken capsule method of chemistry to carry out the capsule fragmentation, calculate the thalline quantity in the capsule through flat band method then; Capsule intensity adopts positive pressure maniputation to measure, and changes the concentration of the best protection effect of confirming each factor through cell concentration and capsule intensity.Single factor result of study shows; The interpolation of glucose can have provide protection to the thalline activity, but the variation of concentration is little to the influence of thalline active protection, and is little to the capsule intensity influence; From reducing cost and to the comprehensive angle of thalline provide protection, the concentration of selecting glucose for use is 3%.The variation of the concentration of sucrose, glycerine and lime carbonate has considerable influence to the variation of cell density and capsule intensity.The experiment of single factor result has shown that also the preferred concentration of sucrose is 5%, and the preferred concentration of glycerine is 15%, and the preferred concentration of lime carbonate is 0.2%.On the basis of single factor analysis, select sucrose, glycerine and three factors of lime carbonate for use, design 3 factors, 3 horizontal quadratures test L
9(3
3); The high-density microcapsule leaven for preparing put into by the protection liquid for storage of orthogonal design under 4 ℃ of conditions, preserved one month; Adopt the broken capsule method of chemistry to carry out the capsule fragmentation, calculate the thalline quantity in every gram capsule through flat band method then, adopt positive pressure maniputation to measure capsule intensity; Confirm best storage liquid prescription through cell concentration and capsule intensity variation with a level difference analysis, concrete experimental design scheme and result are shown in table 1 and table 2.
Table 1L
9(3
3) orthogonal experiment factor and water-glass
Table 2L
9(3
3) orthogonal experiment scheme and result
Learning that from the big I of extreme difference R the principal element that influences cell concentration is a sucrose content, secondly is glycerine and calcium carbonate content, and the primary and secondary of each factor is A＞B＞C in proper order, and best storage liquid prescription is A
2B
2C
3The principal element that influences capsule intensity is a calcium carbonate content, secondly is glycerine and sucrose content, and the primary and secondary of each factor is C＞A＞B in proper order, and best storage liquid prescription is A
2B
1C
2Lime carbonate is the active minor effect factor of thalline, but is major influence factors to capsule intensity, considers the active combined influence with capsule intensity to thalline of each factor, will reduce cost simultaneously, finally confirms that the storage protection liquid formula of the best is A
2B
1C
2Comprehensive monofactorial experimental result, the end formulation of protection liquid for storage are sodium-chlor 0.9%, glucose sugar 3%, sucrose 5%, glycerine 10%, lime carbonate 0.2%.
With the sub-water preparation of going or staying protection liquid for storage, place 121 ℃ of sterilization 20min according to this prescription, (cell density is 1.6 * 10 with the high-density for preparing then
11Cfu/g) microcapsule leaven is put into protection liquid for storage and under 4 ℃ of conditions, is preserved, and sterilized water is done storage liquid and compared simultaneously.Around each cell density in the capsule and capsule intensity are detected, Fig. 1 is seen in the variation of cell density and capsule intensity.As can beappreciated from fig. 1, protection liquid for storage has good protection effect to thalline activity in the capsule, and cell density is 2.4 * 10 in 24 all backs capsules
10Cfu/g, and capsular cell density is 3.8 * 10 in the sterilized water
3Cfu/g; It can also be seen that from Fig. 1 protection liquid for storage can effectively slow down the reduction of capsule intensity, after 24 weeks, the capsule intensity in the storage liquid is 2.05N, and the capsule intensity in the sterilized water is merely 1.12N.Above result shows that the prescription of capsule storage protection liquid of the present invention has thalline activity in the good protection capsule, and the reduction that can effectively slow down capsule intensity.The present invention can effectively prolong the storage and the time limit of service of capsule starter, and improves the international competitiveness of China's capsule starter product.
Claims (1)
1. a protection liquid for storage of microcapsule leaven is characterized in that, is the protection liquid on basis with saline water, contains and only contain 3% glucose simultaneously, 5% sucrose, 10% glycerine and 0.2% lime carbonate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101857150A CN101869138B (en) | 2010-05-28 | 2010-05-28 | High-efficiency protection liquid for storage of microcapsule leaven |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101857150A CN101869138B (en) | 2010-05-28 | 2010-05-28 | High-efficiency protection liquid for storage of microcapsule leaven |
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| Publication Number | Publication Date |
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| CN101869138A CN101869138A (en) | 2010-10-27 |
| CN101869138B true CN101869138B (en) | 2012-05-30 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104313005B (en) * | 2014-10-22 | 2018-10-30 | 江苏省农业科学院 | A kind of high stability lactobacillus micro-capsule and the preparation method and application thereof |
| CN104818237A (en) * | 2015-05-29 | 2015-08-05 | 北京根来福农业科技有限公司 | Microbial preparation, OPS biological slow-release agent containing microbial preparation and preparation method of microbial preparation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554741A (en) * | 2003-12-24 | 2004-12-15 | 广东省农业科学院农业生物技术研究所 | Stabilizing post-processing method for beneficial microbial preparation |
| CN1844352A (en) * | 2006-05-12 | 2006-10-11 | 河北农业大学 | Composite protector for high activity bifidobacteria dry powder formulation |
| CN1847390A (en) * | 2006-05-12 | 2006-10-18 | 河北农业大学 | Composite protectant for freeze dried lactobcillus fermenting prepn |
| WO2007128829A3 (en) * | 2006-05-09 | 2008-01-03 | Biotica Tech Ltd | 18,21-didesoxymacbecin derivatives for the treatment of cancer |
| CN101691559A (en) * | 2009-10-19 | 2010-04-07 | 李军训 | Vacuum freeze-drying method for probiotic lactobacillus |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554741A (en) * | 2003-12-24 | 2004-12-15 | 广东省农业科学院农业生物技术研究所 | Stabilizing post-processing method for beneficial microbial preparation |
| WO2007128829A3 (en) * | 2006-05-09 | 2008-01-03 | Biotica Tech Ltd | 18,21-didesoxymacbecin derivatives for the treatment of cancer |
| CN1844352A (en) * | 2006-05-12 | 2006-10-11 | 河北农业大学 | Composite protector for high activity bifidobacteria dry powder formulation |
| CN1847390A (en) * | 2006-05-12 | 2006-10-18 | 河北农业大学 | Composite protectant for freeze dried lactobcillus fermenting prepn |
| CN101691559A (en) * | 2009-10-19 | 2010-04-07 | 李军训 | Vacuum freeze-drying method for probiotic lactobacillus |
Non-Patent Citations (3)
| Title |
|---|
| Zdenek hubalek.Protectants used in the cryopreservation of microorganisms.《Cryobiology》.2003,第46卷第205-229页. * |
| 周剑忠 等.液芯微胶囊制备条件优化.《江西农业学报》.2009,第21卷(第8期),第138-141页. * |
| 杜磊 等.乳酸菌冷冻保护剂选择的研究.《乳业科学与技术》.2010,(第3期),第119-121. * |
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