CN1539402A - Aqueous solution extracted from nucleus of hawkthom as fluid for proteting teeth - Google Patents
Aqueous solution extracted from nucleus of hawkthom as fluid for proteting teeth Download PDFInfo
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- CN1539402A CN1539402A CNA2003101034045A CN200310103404A CN1539402A CN 1539402 A CN1539402 A CN 1539402A CN A2003101034045 A CNA2003101034045 A CN A2003101034045A CN 200310103404 A CN200310103404 A CN 200310103404A CN 1539402 A CN1539402 A CN 1539402A
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- dental care
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Abstract
A tooth-protecting liquid is prepared from haw kernel through dry distillation or rectification, and oil-water separation to obtain the aqueous solution. Its advantages are high curative effect to periodontitis and gingivitis, and no toxic by-effect.
Description
Technical field
The present invention relates to the application of the aqueous solution of Semen Crataegi extraction, particularly relate to a kind of aqueous solution that extracts with Semen Crataegi, be used for the human oral cavity tooth protection, the product of prevention and treatment oral disease as dental care liquid.
Background technology
Medical institutions are used for cleaning teeth at present, sterilization removes speckle, promptly adopt physical methods such as ultrasonic cavitron, this method is obvious to the overglaze side effect of tooth, and can not play effect to the disappearance of symptoms such as periodontal disease, gingivitis, therefore, be badly in need of research and find new application, to solve the problem that exists at present with known substance.
Summary of the invention
Purpose of the present invention is the shortcoming and deficiency in order to overcome above-mentioned prior art just, use known product and provide, it is a kind of aqueous solution product that has no side effect from the extraction of natural plants Semen Crataegi, be used for the cleaning teeth sterilization and remove speckle, diseases such as periodontal disease, gingivitis had good treatment function, simultaneously also for preventing and treating oral disease and the tooth that keeps healthy has found a kind of ideal method, Here it is dental care liquid (trade name " Ya Baolu " dental care liquid).
The present invention realizes by following technical proposal:
On November 20th, 1996, the inventor applied for a patent, and the patent No. is 96120686.1, and denomination of invention " is extracted the method for disinfectant " from Semen Crataegi, grant patent.Simultaneously, applied for " cure verrucosis and gonorrhea disinfectant " again, patent No. 96120687.X is used for the disinfectant of skin, gynecological, also grants patent.This disinfectant, it is to obtain the aqueous solution of colourless oil-water separation with the Semen Crataegi dry distilling through rectification that the scientific and technical personnel development of easy biotechnology institute, these two patents are arranged greatly by Beijing, treats skin, gynaecopathia.The present invention finds new purposes to this aqueous solution, can be used for the oral cavity, makes dental care liquid and uses.
A kind of " Ya Baolu " dental care liquid, it contains Fumaric acid, ursolic acid, oleanolic acid, succinic acid, phenol etc., and each constituent content is: (percetage by weight is represented)
Phenol 20-25, ursolic acid 1-3, oleanolic acid 2-4, Fumaric acid 2-3, succinic acid 2-3, hydroquinone 10-15,3.5-methyl-IH-pyrazoles 25-30,2 furan carboxyaldehyde 20-30, water 0.05-0.1, other 1-3.
The pH value of " Ya Baolu " of the present invention dental care liquid is 2-3, proportion 0.988-1.100g/ml.
" Ya Baolu " of the present invention dental care liquid through the test of acute toxicity per os, mutagenicity test, quantitatively kill the staphylococcus aureus test, quantitatively kill the test of coli test and content of beary metal, existing division is as follows:
1, acute toxicity per os test:
Being tried thing is " Ya Baolu " of the present invention dental care liquid.Result of the test sees Table 1:
| Sex | Dosage grouping (mg/kg) | Number of animals (only) | Dead animal number (only) | Mortality rate (%) |
| Male | ????2150 ????4640 ????10000 ????21500 | ????5 ????5 ????5 ????5 | ????0 ????2 ????2 ????5 | ????0 ????40 ????40 ????100 |
| Female | ????2150 ????4640 ????10000 ????21500 | ????5 ????5 ????5 ????5 | ????1 ????0 ????3 ????5 | ????20 ????0 ????60 ????100 |
Conclusion: after the contamination, poisoning and death condition appears in the high dose group animal.Ya Baolu (dental care liquid) is to its mouse oral LD
50And 95% credibility interval is respectively: male 9090mg/kg body weight, 5660-14600mg/kg body weight; Female 7940mg/kg body weight, the 4670-13500mg/kg body weight the results showed, belongs to actual nontoxic level by acute toxicity grading criteria.
2, mutagenicity test:
Being tried thing is " Ya Baolu " of the present invention dental care liquid, the results showed that this is tried thing to the undistorted effect of bone marrow polychromatic erythrocyte chromosome.Result of the test sees Table 2, table 3:
Table 2: tried the influence of thing to male mice bone marrow polychromatic erythrocyte micronuclear rates
| Tried thing | Sex | Dosage (mg/kg) | Number of animals (only) | The polychromatic erythrocyte number | Contain micronucleus polychromatic erythrocyte number | Micronuclear rates (‰) | PH value |
| Tried thing | Male | ??1000 ??2000 ??5000 | ???5 ???5 ???5 | ??5000 ??5000 ??5000 | ????4 ????2 ????3 | ???0.8 ???0.4 ???0.6 | ＞0.05 ＞0.05 ＞0.05 |
| Negative control | Male | ??10000 | ???5 | ??5000 | ????3 | ???0.6 | - |
| Cyclophosphamide | Male | ??60 | ???5 | ??5000 | ????124 | ???24.8 | ＜0.01 |
Table 3: tried the influence of thing to female mice bone marrow polychromatic erythrocyte micronuclear rates
| Tried thing | Sex | Dosage (mg/kg) | Number of animals (only) | The polychromatic erythrocyte number | Contain micronucleus polychromatic erythrocyte number | Micronuclear rates (%) | PH value |
| Tried thing | Female | ???1000 ???2000 ???5000 | ???5 ???5 ???5 | ??5000 ??5000 ??5000 | ????5 ????2 ????6 | ??1.0 ??0.4 ??1.2 | ＞0.05 ＞0.05 ＞0.05 |
| Negative control | Female | ???10000 | ???5 | ??5000 | ????3 | ??0.6 | - |
| Cyclophosphamide | Female | ???60 | ???5 | ??5000 | ????105 | ??21.0 | ＜0.01 |
Conclusion: learn check by statistics, the result shows: this is tried thing the PCEMNR micronuclear rates is not seen obvious increase, compare there was no significant difference (P＞0.05) with negative control group, and positive thing matched group of cyclophosphamide and negative control group are relatively, significant differences (P＜0.01) is arranged, illustrate that this is tried thing to the undistorted effect of PCEMNR chromosome.
3, quantitatively kill the staphylococcus aureus test
Tried thing with 1.Bacterial strain: staphylococcus aureus ATCC6538 (6-8 generation).In dental care liquid stock solution, contain 0.3% lecithin, the 0.03mol/L PBS of 3% tween 80.
Result of the test
1. nertralizer qualification test
Show through three repeated trials results, first group of clump count of on average growing be 0cfu/ sheet, second group of clump count of on average growing be the 26cfu/ sheet, third and fourth, five groups of clump counts of on average growing are respectively 2.23 * 10
6Cfu/ sheet, 2.36 * 10
6Cfu/ sheet and 2.17 * 10
6The cfu/ sheet.Error rate is 3.12% between three groups.(table 4).
Table 4: Ya Baolu (dental care liquid) nertralizer qualification test result
| Group | Each time test growth bacterium number (cfu/ sheet) | Average growth clump count (cfu/ sheet) | ||
| ??1 | 2 | ??3 | ||
| ????1 | ??0 | 0 | ??0 | ????0 |
| ????2 | ??45 | 12 | ??20 | ????26 |
| ????3 | ??2.87×10 8 | 2.05×10 6 | ??1.77×10 6 | ????2.23×10 6 |
| ????4 | ??2.62×10 8 | 2.25×10 6 | ??2.20×10 6 | ????2.36×10 6 |
| ????5 | ??2.95×10 8 | 1.75×10 6 | ??1.80×10 6 | ????2.17×10 6 |
Annotate: the negative control asepsis growth
2. Ya Baolu (dental care liquid) is to the result that kills of staphylococcus aureus
Ya Baolu (dental care liquid) stock solution, effect 0.5min, 1min and 2min are 99.99%, 100% and 100% (table 5) to the average kill ratio of staphylococcus aureus.
Table 5: Ya Baolu (dental care liquid) is to the result that kills of staphylococcus aureus
| Concentration | Effect different time (min) on average kill logarithm value | Average control bacterium number and scope * 10 6(cfu/ sheet) | ||
| ????0.5 | ?????1 | ????2 | ||
| Ya Baolu (dental care liquid) stock solution | ??99.99 ??(99.99-99.9) | ??100 ??(100-100) | ??100 ??(100-100) | 1.44 (0.63-2.99) |
Annotate: the negative control asepsis growth
The result shows:
1, Ya Baolu (dental care liquid) stock solution, the 0.03mol/LPBS that contains 0.3% lecithin, 3% tween 80, effectively in the neutralization solution and this dental care liquid of thalline remained on surface to the effect of staphylococcus aureus, and nertralizer and neutralized reaction product have no adverse effects to staphylococcus aureus and culture medium.
2, Ya Baolu (dental care liquid) stock solution, effect 0.5min is 99.99% to the average kill ratio of staphylococcus aureus.
4, quantitatively kill coli test
Tried thing with 1, bacterial strain: escherichia coli 8099 (6-8 generation), nertralizer: the 0.03mol/L PBS (table 6) of 0.3% lecithin, 3% tween.
Table 6: Ya Baolu (dental care liquid) is to the colibacillary result that kills
| Concentration | Effect different time (min) on average kill logarithm value | Average control bacterium number and scope * 10 6(cfu/ sheet) | ||
| ??0.5 | ????1 | ????2 | ||
| Ya Baolu (dental care liquid) stock solution | ??99.99 ??(99.99-99.9) | ??100 ??(100-100) | ??100 ??(100-100) | 1.44 (0.83-2.51) |
Annotate: the negative control asepsis growth
Conclusion shows:
Ya Baolu (dental care liquid) stock solution, effect 0.5min is 99.99% to colibacillary average kill ratio.
5, lead, hydrargyrum, the test of arsenic content:
Being tried thing is " Ya Baolu " of the present invention dental care liquid, and test shows that this is tried the lead of thing, hydrargyrum, arsenic content and all is lower than the requirement of limiting the quantity of.Result of the test table 7.
Table 7:
| The mensuration project | Unit | Measurement result | Regulation and stipulation |
| Plumbous (Pb) | mg/kg | ＜0.5 | ≤40 |
| Hydrargyrum (Hg) | mg/kg | 0.012 | ≤1 |
| Arsenic (As) | mg/kg | ＜0.1 | ≤0 |
Conclusion: lead, hydrargyrum, three hygienic chemistry indexs of arsenic all meet the requirement of limiting the quantity of of lead, hydrargyrum, arsenic in " cosmetics health standard " (version in 2002) in the sample.
More than test detects by Institute for Environment Hygiene and Health Related Product Safety, China CDC.
" Ya Baolu " of the present invention dental care liquid can have high-strength killing action to intraoral antibacterial, virus, and oral disease is had specially good effect.
Using method: product splashed into gargle five minutes in the mouth.Do not need clear water to gargle.Once a day, 5-7 days is one-period, and also available cotton swab dips in this product and smears infection site in the oral cavity, once a day, and each five minutes.
Compared with the prior art the present invention has following advantage: easy to use, effectively the broad-spectrum therapeutic effect has no side effect, and especially tooth whitening, periodontitis, gingivitis is had specially good effect, the cure rate height.Belong to pure natural extract type oral health products.
Specific implementation method
Enumerate an embodiment below, the present invention is further specified, but the present invention is not only limited to this embodiment.
Embodiment 1: the Semen Crataegi collected through the washing dewatering, is carried out a dry distilling under 100 ℃ ± 2 ℃ temperature, improve temperature to 250 ℃ ± 2 ℃ again and continue dry distilling, will heat up in a steamer the liquid that obtains and obtain water-soluble products liquid through rectification.This product is carried out clinical observation, and its result is as follows:
Example one: Lee XX, man, 78 years old, course of disease several years.Tooth tea, smoke puff, gingiva are hemorrhage often, and tooth has loosening sense.Use this product after one week, tooth tea, smoke puff obviously disappear.Gingival hemorrhage does not take place again, and the odontoseisis sense is eliminated.
Example two: rope XX, the man, 50 years old, periodontitis often took place, with hemorrhage.Use this product after two days, periodontal disease recovery from illness, never recurrence.Tooth obviously bleaches.
Example three: all XX, woman, 46 years old.Gingiva is often hemorrhage, and tooth is met hot and cold tingle, uses this product after three days, and it is normal that tooth recovers, and gingival hemorrhage never recurs.
Product of the present invention is used for the oral cavity tooth protection, prevention and treatment oral disease.
Claims (1)
1, the aqueous solution that extracts with Semen Crataegi is used for the human oral cavity tooth protection, prevention and treatment oral disease as dental care liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310103404 CN1247185C (en) | 2003-10-31 | 2003-10-31 | Aqueous solution extracted from nucleus of hawkthom as fluid for proteting teeth |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310103404 CN1247185C (en) | 2003-10-31 | 2003-10-31 | Aqueous solution extracted from nucleus of hawkthom as fluid for proteting teeth |
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| CN1539402A true CN1539402A (en) | 2004-10-27 |
| CN1247185C CN1247185C (en) | 2006-03-29 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982085A (en) * | 2010-09-08 | 2011-03-02 | 合肥工业大学 | Tooth-care soft candy and preparation method thereof |
| CN111184674A (en) * | 2020-01-17 | 2020-05-22 | 苏州秀欣生物科技有限公司 | Oral care agent for superficial wound care and application method thereof |
| WO2021035378A1 (en) * | 2019-08-23 | 2021-03-04 | Colgate-Palmolive Company | Method to incorporate oleanolic acid into oral care compositions and oral care compositions based thereof |
-
2003
- 2003-10-31 CN CN 200310103404 patent/CN1247185C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982085A (en) * | 2010-09-08 | 2011-03-02 | 合肥工业大学 | Tooth-care soft candy and preparation method thereof |
| CN101982085B (en) * | 2010-09-08 | 2012-06-13 | 合肥工业大学 | Tooth-care soft candy and preparation method thereof |
| WO2021035378A1 (en) * | 2019-08-23 | 2021-03-04 | Colgate-Palmolive Company | Method to incorporate oleanolic acid into oral care compositions and oral care compositions based thereof |
| CN114286664A (en) * | 2019-08-23 | 2022-04-05 | 高露洁-棕榄公司 | Method for incorporating caryophyllin into oral care composition and oral care composition based on same |
| CN111184674A (en) * | 2020-01-17 | 2020-05-22 | 苏州秀欣生物科技有限公司 | Oral care agent for superficial wound care and application method thereof |
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| Publication number | Publication date |
|---|---|
| CN1247185C (en) | 2006-03-29 |
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