CN1190183C - Safety toothpaste for preventing and curing oral cavity disease - Google Patents
Safety toothpaste for preventing and curing oral cavity disease Download PDFInfo
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- CN1190183C CN1190183C CNB011364866A CN01136486A CN1190183C CN 1190183 C CN1190183 C CN 1190183C CN B011364866 A CNB011364866 A CN B011364866A CN 01136486 A CN01136486 A CN 01136486A CN 1190183 C CN1190183 C CN 1190183C
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Abstract
The present invention discloses safety toothpaste which is prepared from IgY rough agents, Caborpl, sodium alginate, sodium carboxymethylcellulose, glycerol, sorbose alcohol, aspartame, peppermint essence, fragrant citrus essence, honey peach essence and Chinese cymbidium. The present invention can directly inhibit pathogens relevant to oral diseases, does not contain harmful substances and is a kind of edible toothpaste that is safe for deglutition.
Description
Technical field
The present invention relates to a kind of toothpaste, particularly a kind of safety toothpaste of preventing and treating oral disease.
Background technology
Its prescription of toothpaste on the current domestic and international market is all similar, the chemical substance that all contains infringement human body health, as dodecyl sodium sulfate (SDS), dodecanoyl sodium sarcosinate, dodecyl ethylene glycol amide, sodium polyacrylate etc., can not swallow when therefore brushing teeth with toothpaste.But, studies show that the adult brushes teeth, the 15-20% of each consumption will be swallowed by mistake; The child brushes teeth, and then about 40% of each consumption will be swallowed by mistake.Life-time service certainly will endanger healthy, especially very important to children's potential impact.And general on the market anti-caries toothpaste, the overwhelming majority is tendentious, unique effective toothpaste with fluoride also can not effect a permanent cure, and there are many problems in fluorine: 1) use fluoride in caries prevention and caused artificial dental fluorosis popular.Successively add fluoride in caries prevention in tap water as China Hong Kong (1961), Guangzhou (nineteen sixty-five), the result has caused that dental fluorosis is popular; 2) Liu Yanling etc. uses continuous 2 months tracing study prompting of toothpaste with fluoride to the child, because the child uses the widely different of amount of paste, the situation that mistake is swallowed toothpaste often takes place again, cause the part child to take in the fluorine that is higher than for fluorine amount standard, this is very big to children's torso health (particularly living in the child of high fluoride area and fluorine affinity zone) harm; 3) prolonged application of fluoride can cause cariogenic bacteria variation and produce the bacterial strain of anti-the fluorine.The not only anti-fluorine of this bacterial strain, and cariogenic potential is stronger, causes dental caries to increase the weight of.The popular on the market toothpaste that lays claim to anti-periodontal disease or eliminate the halitosis effect allly truly has a little effects, and great majority all contain the phosphoramidite chemical antibacterial.Because this class chemical bactericide has also been killed probiotics in the oral cavity in the lump, certainly will destroy flora ecological balance in the oral cavity.After brushing just, though of short duration effective, spend several hours, pathogenic bacterium can breed again again in a large number.And, often use this toothpaste that contains the phosphoramidite chemical antibacterial, can make pathogenic bacterium generation drug resistance in the oral cavity, in the course of time, the state of an illness increases the weight of on the contrary.Domestic and international existing IgY slightly puies forward technology, and some is to take the organic solvent method, as with sad, or ethanol, or acetone, or chloroform etc.This method has certain influence to the IgY activity, and can exist chemical substance residual, influences environment and healthy.Have plenty of the employing sedimentation method; As Polyethylene Glycol, dextran sulfate etc., shortcoming is that yield is lower, and has the organic chemicals residue problem too.Has plenty of water dilution method.The many technological processes of this method advantage are simple, the productive rate height, and cost is low, does not particularly have any chemical substance and pollutes, and safety is good; Shortcoming is that lipid is removed not exclusively.
Summary of the invention
The technology that the purpose of this invention is to provide the anti-various pathogens IgY crude preparation by using of a kind of new improved preparation; Another object of the present invention provide a kind of brand-new prescription, directly suppress oral disease related diseases substance, do not have chemical harmful substance, and deglutible safe edible type toothpaste.
The new preparation process of the relevant IgY crude preparation by using of the present invention is as follows:
According to causing dental caries, periodontal disease, mouthful pathogen because of the property halitosis, turn out pathogenic bacterium according to a conventional method, and mix by equal proportion, add the freund adjuvant of equivalent again, handle with high speed homogenizer, make somatic antigen.Getting this antigen carries out subcutaneous or the wing intravenous injection to bird inlay (a kind of through breeding chicken experiment screening, the high immunologic responsiveness of tool); Every two weeks once, amount to three times.
The immunity egg takes out egg yolk with the egg yolk sieve, beats evenly, adds the distilled water diluting mixing of 4-6 times of volume, and adjust pH is to 4.5-6.5, and adding 2% food is 1.0 ‰-2.0 ‰ with sodium alginate to final concentration, stirs until turbidity and precipitation occurring, adds 2% food CaCl
2Liquid to final concentration is 0.5 ‰-1.0 ‰, stirs, and 2-6 ℃ is spent the night, and supernatant is collected in siphon, 8, and 000-12, centrifugal 20 minutes of 000rpm gets supernatant, ultrafiltration and concentration, 0.22 μ membrane filtration degerming, lyophilization promptly gets corresponding IgY crude preparation by using.
The preparation technology of IgY safety toothpaste of the present invention is as follows:
The IgY crude preparation by using 0.01-0.1 weight portion of the present invention of filling a prescription, Ka Bopuer (Caborpl) 0.5-1.0 weight portion, sodium alginate (sodium alginate) 0.2-0.3 weight portion, sodium carboxymethyl cellulose (sodiumcarboxymethvlcellulose) 0.5-1.0 weight portion, glycerol 10.0-12.0 weight portion, Sorbitol (sorbit01) 8.0-10.0 weight portion, aspartame (aspartame) 0.12-0.15 weight portion, Herba Menthae essence 0.2-0.5 weight portion, fragrant citrus essence 0.1-0.2 weight portion, honey peach essence 0.1-0.2 weight portion, pandan paste 0.2-0.5 weight portion.Above raw material is edible-type.According to product storage period length, can add the edible-type antiseptic in right amount.
Technological process adds sodium alginate, sodium carboxymethyl cellulose in the distilled water of 60 parts of by weight/volume, and the swelling dissolving adds other composition again, fully stir, make mix homogeneously, add distilled water at last and be settled to 100ml, the gelation paste that stirs, flexible pipe reinjects.
IgY crude preparation by using experimentation of the present invention is as follows:
1) long term toxicity test: select the healthy SD rat for use, feed with IgY of the present invention, every day the 80mg/kg body weight; 30d.The result: rat does not have any clinical symptoms; Blood, routine urianlysis are normal; Liver, kidney function test are normal; Pathological anatomy checks that brain, the heart, liver, kidney, testis do not have any anomaly.
2) the active detection: indirect hemagglutination inhibition test is tired and is reached 1: 512; ELISA reaches 204800 unit prices.
3) suppress to adhere to experiment: dilution in 1: 8, the adhesive attraction that still has oral disease pathogen such as obvious suppression mutans streptococcus.
4) animal preventing decayed tooth experiment (IgY crude preparation by using group of the present invention, each 10 rat of matched group) result feeds the rat that infects mutans streptococcus with IgY crude preparation by using of the present invention, can prevent the generation of dental caries effectively.See the following form:
| Matched group | Experimental group | |
| Only enamel caries damage dental caries damage is deep to dentin 1/4 dental caries damage and is deep to whole dentin | 47 25 8 | 19 0.57 0 |
Statistical analysis, P＜0.01, two group tool significant differences.
5) crowd observes: volunteer 45 people, be divided into two groups at random, and carried out 21d preventing decayed tooth clinical observation by a definite date with IgY crude preparation by using of the present invention (safety toothpaste), placebo respectively.Observed content is the white proportion by subtraction that mutans streptococcus accounts for total anaerobe in the dental plaque before and after experiment.The results are shown in following table:
| Experiment back P value before the experiment |
| IgY crude preparation by using group 50.8 22.3＜0.01 comforts organize 54.1 50.7 0.242 |
Before and after learning processing IgY crude preparation by using group experiment of the present invention by statistics significant differences is arranged, this shows that IgY crude preparation by using of the present invention (crude preparation by using and safety toothpaste) can obviously suppress the growth of mutans streptococcus in the dental plaque, and the specificity anticaries action is arranged.
The inventive method prepares the IgY crude preparation by using and has active height, high specificity, good effect, application safety, and advantages such as technology is simple, cost is low, non-environmental-pollution; In addition, other composition of eggshell, Ovum Gallus domesticus album and egg yolk all can obtain comprehensive utilization.
IgY safety toothpaste of the present invention is exactly at the weak point of current toothpaste and the brand-new type toothpaste that research design comes out is characterized in: main component is directly at the immune formulation IgY of pathogen, and without any side effects; Formulation of tooth-paste and existing formulation of tooth-paste are different fully, are a kind of edible toothpaste, do not have any harmful chemical; Specificity is at pathogen, and prevention effect is remarkable: safety is good, can swallow, and is also passable even do not gargle in the back of brushing teeth, and uses and becomes convenient, and travelling all suits at home, is particularly useful for the child.The present invention is the special oral mucosa absorbent (slow releasing agent) that adds in composition, as Caborpl (Ka Bopuer) etc.IgY time of staying on oral mucosa and facing is prolonged, strengthened the action time of suppressing pathogenic bacterium, strengthen its effect.Toothpaste dental caries of the present invention, periodontal disease, mouth have good prevention effect because of the property halitosis.
Embodiment 1:
According to causing dental caries, periodontal disease, mouthful pathogen because of the property halitosis, turn out pathogenic bacterium according to a conventional method, and mix by equal proportion, add the freund adjuvant of equivalent again, handle with high speed homogenizer, make somatic antigen.Getting this antigen 1 .0ml carries out subcutaneous or the wing intravenous injection to bird inlay (a kind of through breeding chicken experiment screening, the high immunologic responsiveness of tool); Every two weeks once, amount to three times.
The immunity egg takes out egg yolk with the egg yolk sieve, beats evenly, adds the distilled water diluting mixing of 5 times of volumes, adjust pH to 5.5, and adding 2% food is 1.5 ‰ with sodium alginate to final concentration, stirs until turbidity and precipitation occurring, adds 2% food CaCl
2Liquid to final concentration is 0.75 ‰, stirs, and 4 ℃ are spent the night, and supernatant is collected in siphon, 10, and centrifugal 20 minutes of 000rpm gets supernatant, ultrafiltration and concentration, 0.22 μ membrane filtration degerming, lyophilization promptly gets corresponding IgY crude preparation by using.
Embodiment 2:
The IgY crude preparation by using 0.025 of the present invention of filling a prescription restrains, Ka Bopuer (Caborpl) 0.5 gram, sodium alginate (sodium alginate) 0.2 gram, sodium carboxymethyl cellulose (sodium carboxymethvlcellulose) 0.8 gram, glycerol 10.0 grams, Sorbitol (sorbit01) 8.0 grams, aspartame (aspartame) 0.12 gram, Herba Menthae essence 0.3 gram, fragrant citrus essence 0.2 gram, honey peach essence 0.1 gram, pandan paste 0.3 gram.Above raw material is edible-type.According to product storage period length, can add the edible-type antiseptic in right amount.
Technological process adds sodium alginate, sodium carboxymethyl cellulose in the distilled water of 60ml, and the swelling dissolving adds other composition again, fully stirs, and makes mix homogeneously, and add distilled water at last and be settled to 100ml, the gelation paste that stirs, flexible pipe reinjects.
Claims (4)
1, a kind of safety toothpaste, it is characterized in that what this safety toothpaste was made up of the prescription of following weight portion: IgY crude preparation by using 0.01-0.1 weight portion, Ka Bopuer 0.5-1.0 weight portion, sodium alginate 0.2-0.3 weight portion, sodium carboxymethyl cellulose 0.5-1.0 weight portion, glycerol 10.0-12.0 weight portion, Sorbitol 8.0-10.0 weight portion, aspartame 0.12-0.15 weight portion, Herba Menthae essence 0.2-0.5 weight portion, fragrant citrus essence 0.1-0.2 weight portion, honey peach essence 0.1-0.2 weight portion, pandan paste 0.2-0.5 weight portion; Wherein the IgY crude preparation by using is to be made by following method:
According to causing dental caries, periodontal disease, mouthful pathogen because of the property halitosis, turn out pathogenic bacterium according to a conventional method, and mix by equal proportion, add the freund adjuvant of equivalent again, handle with high speed homogenizer, make somatic antigen; Getting this antigen carries out subcutaneous or the wing intravenous injection to bird inlay; Every two weeks once, amount to three times; The immunity egg takes out egg yolk with the egg yolk sieve, beats evenly, adds the distilled water diluting mixing of 4-6 times of volume, and adjust pH is to 4.5-6.5, and adding 2% food is 1.0 ‰-2.0 ‰ with sodium alginate to final concentration, stirs until turbidity and precipitation occurring, adds 2% food CaCl
2Liquid to final concentration is 0.5 ‰-1.0 ‰, stirs, and 2-6 ℃ is spent the night, and supernatant is collected in siphon, 8, and 000-12, centrifugal 20 minutes of 000rpm gets supernatant, ultrafiltration and concentration, 0.22 μ membrane filtration degerming, lyophilization promptly gets corresponding IgY crude preparation by using.
2, safety toothpaste according to claim 1, it is characterized in that described prescription consists of: IgY crude preparation by using 0.025 weight portion, Ka Bopuer 0.5 weight portion, sodium alginate 0.2 weight portion, sodium carboxymethyl cellulose 0.8 weight portion, glycerol 10.0 weight portions, Sorbitol 8.0 weight portions, aspartame 0.12 weight portion, Herba Menthae essence 0.3 weight portion, fragrant citrus essence 0.2 weight portion, honey peach essence 0.1 weight portion, pandan paste 0.3 weight portion.
3, safety toothpaste according to claim 1 and 2 is characterized in that the preparation technology of described IgY crude preparation by using is:
According to causing dental caries, periodontal disease, mouthful pathogen because of the property halitosis, turn out pathogenic bacterium according to a conventional method, and mix by equal proportion, add the freund adjuvant of equivalent again, handle with high speed homogenizer, make somatic antigen; Getting this antigen 1 .0ml carries out subcutaneous or the wing intravenous injection to bird inlay; Every two weeks once, amount to three times; The immunity egg takes out egg yolk with the egg yolk sieve, beats evenly, adds the distilled water diluting mixing of 5 times of volumes, adjust pH to 5.5, and adding 2% food is 1.5 ‰ with sodium alginate to final concentration, stirs until turbidity and precipitation occurring, adds 2% food CaCl
2Liquid to final concentration is 0.75 ‰, stirs, and 4 ℃ are spent the night, and supernatant is collected in siphon, 10, and centrifugal 20 minutes of 000rpm gets supernatant, ultrafiltration and concentration, 0.22 μ membrane filtration degerming, lyophilization promptly gets corresponding IgY crude preparation by using.
4. the preparation method of safety toothpaste according to claim 2 is characterized in that this method is:
It is that the swelling dissolving adds other composition again in 1: 60 the distilled water by the grams per milliliter ratio that sodium alginate, sodium carboxymethyl cellulose are added by weight/volume, fully stir, make mix homogeneously, add distilled water at last and be settled to 100ml, the gelation paste that stirs, flexible pipe reinjects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011364866A CN1190183C (en) | 2001-10-19 | 2001-10-19 | Safety toothpaste for preventing and curing oral cavity disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011364866A CN1190183C (en) | 2001-10-19 | 2001-10-19 | Safety toothpaste for preventing and curing oral cavity disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1411798A CN1411798A (en) | 2003-04-23 |
| CN1190183C true CN1190183C (en) | 2005-02-23 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011364866A Expired - Fee Related CN1190183C (en) | 2001-10-19 | 2001-10-19 | Safety toothpaste for preventing and curing oral cavity disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1190183C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1482141A (en) * | 2003-08-15 | 2004-03-17 | 雅臣药业集团（远东）有限公司 | Method for preparing specific composite IgY against periodontal disease and group system thereof |
| CN1329091C (en) * | 2005-03-14 | 2007-08-01 | 刘国花 | Use of toothpaste containing anti-tooth decay IgY with dodecyl dimethyl lycine as blowing agent and toothpaste thereof |
| CN105434322B (en) * | 2015-12-22 | 2018-02-27 | 南昌理工学院 | A kind of toothpaste containing oral cavity probiotics |
-
2001
- 2001-10-19 CN CNB011364866A patent/CN1190183C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1411798A (en) | 2003-04-23 |
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Granted publication date: 20050223 |