Summary of the invention:
The purpose of this invention is to provide and a kind ofly utilize the sexual hybridization of branch wheat to breed all good ramiform wheat hybridizing propagation methods of yielding ability, resistance.
The object of the present invention is achieved like this, specifies as follows:
The most important condition of improved seeds is that local natural conditions and cultivation condition are had powerful adaptability, this is decided by parent's adaptability itself to a great extent, select for use and remove wheat 9606 (1) work female parents, divide 33 to make the direction of male parent (ramiform wheat) sexual hybridization as our work.
The wheat hybridizing breeding will be matched good parental combination, and the method for correct seed selection filial generation and the method for fast breeding seed also must be arranged; Filial generation must be according to the various trait between the parent, suitably grasp the strain number of offspring, plantation and the generation religion that separates, type and kind that the comprehensive proterties of seed selection is good, we remove No. 3 former code names (95 of wheat what the branch wheat divided that seed selection is come out in the filial generation of No. 33 X91-1
F2) 21, contrast table reveals stronger growing power in the pedigree method, the new strain of wheat of disease resistance and stable high yield, change the defective of branch wheat starting material resistance difference, strengthen its yielding ability, yield stability, seek a new approach, obtain the stronger kind of volume increase property for wheat breeding, key problem in technology is the filial generation that sexual hybridization obtains meeting breeding objective, and selects on this basis.
Wheat hybridizing breeding concrete steps are as follows:
1. select the parent: select for use wheat to remove 7606 (1) and do maternally, branch wheat 33 is hybridized as male parent; Female parent selects the main fringe of robust plant, only stays 8-16 the small ear in its top, two supermature 2 Xiao Hua of base portion on every small ear;
2. castrate: the flower pesticide among the above-mentioned every Xiao Hua is once gone only, put paper bag immediately and indicate the maternal name of an article and the castration date, column cap maturation after 1-4 days, can pollinate, put paper bag immediately and indicate father, the maternal name of an article and pollination date, the pollination back stripped the rataria cultured in vitro in 18-21 days, and it is carried out adding generation and system's selection;
3. adding generation and system are selected: use booth winter, carry out vernalization with water tank summer, through repeatedly selecting, cultivated comprehensive all good branch hybrid wheats and remove No. 3 former code names (95 of wheat on the basis of the second generation
F2) No. 21 new varieties.
Above-mentioned selection parent is meant: select 7606 (1) work female parents in the hybrid strain garden, divide 33 to make male parent (ramiform wheat), and to select robust plant, just extracted the tassel of leaf sheath out, generally select main fringe for well, cut off maternal tassel earlier and grow slower small ear up and down, then the awn of wheat is cut, about 10 in the general small ear that only stays the middle and upper part, 5 on one side, again the Xiao Hua on each small ear centre that stays to be removed with tweezers, each small ear only stays base portion to grow two Xiao Hua preferably.
Above-mentioned castration is meant: the tassel of putting in order is castrated, utilization is plucked medicine and is castrated method, specific practice: clamp the whole wheat head with left hand thumb and middle finger, the interior coetonium shell that to spend one by one with forefinger presses off gently, the right hand stretches in the Xiao Hua with tweezers three flower pesticide is pressed from both sides out, preferably once go clean, note not hindering column cap and interior coetonium, flower pesticide is not clipped broken simultaneously, if in case clip broken, should extract this Xiao Hua, and clean forceps tips with cotton ball soaked in alcohol, be attached to top pollen to kill, castration should be spent one by one from top to bottom in order and be carried out, do not omit, after the castration, put paper bag immediately, seal, and on paper bag, indicate maternal variety name and castration date, the general 1-4 days column cap maturations in back of castrating can be pollinated, but because kind is sooner or later different with temperature at that time heading stage, the speed of column cap maturation is also inconsistent, and the kind of heading in evening is particularly under hot conditions, castrating the back got final product ripe in 1-2 days, that have in addition when castrating column cap ripe, therefore, pollination in good time should seize a favorable opportunity, to improve ripening rate, the optional branch 33 open tassel flower on every side of the indivedual Xiao Hua in tassel middle and upper part at that time presses off the interior coetonium of Xiao Hua with tweezers, presss from both sides out the flower pesticide of cadmium yellow maturation, puts into capsule, pollination immediately, during pollination, take off the paper bag on the maternal fringe, glue to take a morsel pollen or press from both sides 1-2 ripe flower pesticide with little writing brush and put into each Xiao Hua successively with tweezers; Good in order to guarantee the column cap pollination, flower pesticide is embrocated on column cap gently, after pollination finishes, put paper bag again, and on paper bag, write father, maternal title and pollination date exactly, and stripped the rataria cultured in vitro in back 20 days in pollination, it is carried out Jia Dai and system's selection.
Above-mentioned adding generation is meant: the seed before the sowing is answered the breaking dormancy phase, uses booth winter, and carry out vernalization with refrigerator summer, present domestic winter wheat propagation method, mainly be annually, smart amount of small size or half smart amount breeding, about 100 times of general reproduction coefficient.But branch hybrid wheat of the present invention is removed No. 3 former code names (95 of wheat
F2) No. 21 new varieties, can breed 3-4 generation in 1 year; In the experiment, with the light seeding breeding, with 0.03 kilogram of wheat seeds, the results seed was 4750 kilograms in 2 years, improved 1.5 ten thousand times of reproduction coefficient.Remove No. 3 former code names (95 of wheat
F2) the concrete adding generation method of No. 21 new varieties is as follows:
1. transplant: grasp wheat seeding and move the suitable time that cuts, remove wheat No. 3 in field seeding on September 22, late October digs out 0.2 mu of wheat seeding and is transplanted into 1.1 mu of booths, finishes vernalization stage and grows, must 21-30 days; Require 2-6 ℃ of temperature; Prevention and elimination of disease and pests in time;
2. the slow seedling stage: because root is injured, arranged after cutting out seedling a recovery stage, canopy temperature daytime is the highest to be no more than 10 ℃, is not less than 8 ℃ night, and humidity about 80% is advisable, and 7 days by the slow seedling stage;
3. stand up the stage: behind the slow seedling of wheat, on during to 12 calendars 9 days February 4, canopy temperature is the highest by day to be no more than 15 ℃, is not less than 8 ℃ night, and mean temperature is 12 ℃, and humidity is about 75%; From December 1 to ten days on the 22nd in, canopy temperature is controlled at and the highlyest is no more than 15 ℃, night is minimum to be no less than 10 ℃, daily mean temperature is 12 ℃, December 22, the survey showed that, average every strain increase is tillered 37, has increased by 19% when cutting seedling; The canopy planted agent increases illumination;
4. pull out the minor matters stage: December 23, wheat entered the shooting stage, lasted 34 days, and the temperature in the booth is controlled at and the highlyest is no more than 25 ℃, and night is minimum to be no less than 15 ℃, and daily mean temperature is about 20 ℃; Beginning is the empty technology of useization in time;
5. ear--the filling stage: removed wheat and begin heading for No. 3 in 26 days on the 1st, from January 27 to February 23 days, last 28 days, temperature in the booth is brought up to 25-33 ℃, short its premature ripening, from February 24 canopy temperature bring up to 33-38 ℃, transfer high temperature and force ripe, promptly gather at milking maturity latter stage (February 28), receive 120 jin in seed altogether for 2.4 mu.
Above-mentioned grasp wheat seeding moves the suitable time that cuts and is meant: for No. 3 new varieties of wheat are removed in quick high power breeding, in 2 kilograms every mu of field seedings on September 22, digging seedling by the land for growing field crops every ridge 10 last ten-days period is transplanted to for 0.2 mu and finishes winter wheat vernalization stage in the booth, could normally ear, solid, the present invention removes No. 3 Triticum semiwinternesses of wheat kind, the vernalization preference temperature is the 2-6 degree, the vernalization time is 21-30 days, under the condition of this area, remove wheat No. 3 in sowing on September 22, late October can be finished vernalization and be grown, could guarantee its well-grown, can normally ear, solid;
The above-mentioned slow seedling stage is meant: strict control canopy temperature, delayed when the seedling stage, wheat seeding dug from the ground, because root is injured, after cutting out seedling there is a recovery stage, if this moment, temperature of shed was too high, the aggravation of blade transpiration, radical leaves occurs withered, and influence is grown and is tillered, temperature is too low, seedling-slowing stage prolongs, so, after wheat seeding shifts out, canopy temperature daytime is the highest to be no more than 10 ℃, night minimum being not less than about 8 ℃, humidity about 80% is advisable, and generally lasts 7 days smoothly by the slow seedling stage;
The above-mentioned stage of standing up is meant: behind the slow seedling of wheat, for quickening its growing process, during from February 4 to 12 calendars 9 days, canopy temperature is the highest by day to be no more than 15 ℃, be not less than 8 ℃ night, mean temperature is 12 ℃, and humidity is about 75%, the favourable wheat of such condition is steadily and surely grown, and helps tillering, restraining the growth of seedlings; Wheat rises after one's death, for making its sane growth, increase effective tiller, from December 1 to ten days on the 22nd in, canopy temperature is controlled at and the highlyest is no more than 15 ℃, night is minimum to be no less than 10 ℃, daily mean temperature is 12 ℃, and December 22, the survey showed that, and average every strain increase is tillered 37, ratio has increased by 19% when cutting seedling, lays good basis for striving for more spike number.
Above-mentioned canopy planted agent increases illumination and is meant: wheat is the long-day self pollination crop, especially it is more urgent to the needs of illumination behind the jointing to stand up, in 64 days of wheat 27 days December 23 to February, fluorescent lamp is installed in canopy, to increase illumination, general per 60 square metres a 40W fluorescent lamp is installed, fluorescent tube is apart from about 2 meters on ground, turn on light 6 of every afternoons and turn off the light, increase illumination 14 hours at 8 in morning next day.
Above-mentionedly pull out the minor matters stage and be meant: wheat entered and dialled the joint phase December 23, last 34 days, in order to strive for tillering into more for a long time fringe, the temperature in the booth is controlled at and the highlyest is no more than 25 ℃, and night is minimum to be no less than 15 ℃, daily mean temperature is about 20 ℃, help individual plant and grow stalwartness, it is neat to tiller, and pole is stood upright to fall, ventilation and penetrating light is good, for high yield is established good basis.
Above-mentioned heading--the filling stage is meant: removed wheat and begin heading for No. 3 in 26 days on the 1st, in order to quicken the heading progress and to shorten grouting time, from January 27 to February 23 days, last 28 days, temperature in the booth is brought up to 25-33 ℃, to quicken the accumulation of dry matter in the seed, premature ripening, in order to catch up with the need usefulness in proper seeding March 10, therefore from February 24 canopy temperature bring up to 33-38 ℃, transfer high temperature and force ripe, promptly gather at milking maturity latter stage (February 28), thousand kernel weight has only 30 grams, than normal reduction by 12 grams, receives 120 jin in seed altogether for 2.4 mu.
Above-mentioned prevention and elimination of disease and pests is meant: because booth is few interior sunshine, temperature height, high humidity, permeability are poor, wheat stands up behind the jointing powdery mildew and aphid damage to take place easily, respectively spray 40% triadimefon once February 24 and January 25, concentration is 2/4 to prevent that powdery mildew from taking place, found that worm harm strain rate is all to spray one time with 5000 times of bromine oxygen chrysanthemum ester soups in 29%, 23 on December 24.
Above-mentioned wheat has used the empty culture technique of change to be meant in breeding: the empty cultivation of wheat enforcementization can be regulated and control growing of plant, strengthens its anti-adversity ability, promotes kipeming high yield; Stand up the phase at wheat, 30 kilograms of the paclobutrazol solution of every mu of sprinkling 200ppm can make plant downgrade, and lodging tolerance is strong, and can doublely control wheat powdery mildew and improve the absorption rate to nitrogen of plant; Help the plain 15-20ml of shape in every mu of usefulness of wheat during jointing stage, convert water 50-60 kilogram foliage-spray, it is long to suppress the internode Shen, prevents the later stage lodging; Spray the aqueous solution of sodium bisulfite of 200ppm in wheat booting-pustulation period, spray 2-3 time altogether between booting-pustulation period, each 7-8 days at interval, can improve thousand kernel weight 22 grams, volume increase 10.6%, and can promote filling speed, make full seed, ripen in advance 3-5 days.
No. 3 former code names (95 of wheat are removed in above-mentioned hybridization
F2) feature of 21 new varieties is:
Late-maturing in this kind, the power of emerging is strong, and it is medium to tiller, semiwinterness, anti-early blue or green, seedling partly crawls, well developed root system, and growing power and cold resistance are strong, anti-three rust, banded sclerotial blight, the plant type compactness, appearance is delicate and pretty, jointing ... choose flag phase blade light green color, choose flag rear blade strong green, lift on the leaf rolling, anaphase blade has the point of doing, blade white slightly, the bar maturation of living, the cured matter of stem, go out fringe after, fringe and plant are homochromy, percentage of earbearing tiller height, the hard high resilience of cane, resistant to lodging, hot-dry wind tolerance, spike length 9-11.5cm, wide 1.5-3cm, thick 1.6cm, awn length 4.5cm, (soft awns), white, the sterile lemma ellipse does not have between grain husk, the sharp shape of clever mouth, the particle shape ellipse, grain length 6.1cm, wide 3.5cm has the branch of the ear of grain floriferousness, yield potential is big, and stable high yield is good, single spike number 55-145 grain, different because of the ground soil fertility condition, the degree of lip-rounding is medium, short awns, the white grain of white shell, the thousand kernel weight luffing is little, thousand kernel weight 41 grams, cutin, the unit weight height, the maturing stage falls yellow general.
The ramiform that selection cross of the present invention goes out is removed No. 3 former code names (95 of wheat
F2) 21 new varieties, test its quality through the academy of agricultural sciences, Shandong Province and Ministry of Agriculture food quality supervision verification test center (Jinan): protein content 12.8%, fat 2.1%, wetted surface powder 29.9%, unit weight 935.0.Starch 54.86%, aspartic acid 0.59%, threonine 0.29%, serine 0.49%, glutamic acid 4.07%, glycine 0.49%, alanine 0.44%, cystine 0.19%, valine 0.63%, methionine 0.30%, isoleucine 0.57%, leucine 0.84%, cruel propylhomoserin 0.28%, phenyl alanine 0.77%, lysine 0.38%, ammonia (disregarding) 0.37%, histidine 0.23%, arginine 0.49%, proline 1.11%.This strain is fit to fried and baked goods.High more than 100 grams of the general wheat of volume to weight ratio.
Advantage of the present invention, the one, the cross method science, feasible, the bar maturation of living is its outstanding feature; The 2nd, breed soon, utilize booth to add generation, branch hybrid wheat 95 is adopted in the light seeding breeding
F2--remove No. 3 seeds of wheat and breed for 0.03 kilogram, the results seed was 4750 kilograms in 2 years, improved 1.5 ten thousand times of reproduction coefficient, facts have proved not only good combination property of this kind, and the quick high power reproduction improved variety of energy, so its economic benefit and obvious social benefit; The 3rd, select branch hybrid wheat 95
F2--it is good to remove No. 3 comprehensive proterties of wheat, and the drought resisting alkali tolerance is strong, and it is big to combat a natural disaster potentiality; 2001-2002 year is in Ding Wu township district examination, the 20 centimetres of native saliferous 3% of table in Ding Wu township, and the present invention removes wheat No. 3 than No. 17 volume increase 28.8% in Jinan, than No. 23 volume increase 52% of Shandong wheat.