CN1418889A - Momordi glucose-reducing polypeptide - Google Patents
Momordi glucose-reducing polypeptide Download PDFInfo
- Publication number
- CN1418889A CN1418889A CN 02134773 CN02134773A CN1418889A CN 1418889 A CN1418889 A CN 1418889A CN 02134773 CN02134773 CN 02134773 CN 02134773 A CN02134773 A CN 02134773A CN 1418889 A CN1418889 A CN 1418889A
- Authority
- CN
- China
- Prior art keywords
- glucose
- momordi
- melon seed
- bitter melon
- reducing polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention uses momordic seed as raw material, utilizes organic solvent to defat, and uses alkali to make extraction, and uses acid to make precipitation so as to obtain an active substance momordic glocose-reducing polypeptide. Said inbention can be easily absorbed by human body, can activate insulin in the human body so as to attain reducing blood sugar of human body, so that it has a certain health-care action for diabetics.
Description
What the present invention relates to is the active substance that extracts from bitter melon seed, specifically extracts Momordi glucose-reducing polypeptide in the bitter melon seed.
Some active ingredient in the balsam pear can reduce the blood sugar for human body level, and this is confirmed through many experiments.At present, with the active ingredient lowering blood glucose level of balsam pear method, usually have so two kinds: first kind is directly to carry out compatibility with balsam pear and related drugs, be used for the treatment of diabetes, as publication number is 1277850 Chinese invention patent applications, use balsam pear, the coptis, stiff silkworm, pearl powder and ginseng compatibility exactly together, be used for islet cells is repaired.Second kind is the active ingredient in extracting balsam pear, be prepared into all kinds of preparations with these active ingredients again, be used for the treatment of diabetes, as publication number be 1255044, application number is 98805001.3 Chinese invention patent application, provide a kind of water-soluble monster of carrying of balsam pear to be called MC6, the also peptide composition MC6.1 of MC6 that provides simultaneously and derivative and the stand-in of MC6.1.Above-mentioned these patent applications all are to have utilized the plant balsam pear, and have ignored the utilization to bitter melon seed.Although publication number is 1300569, application number is that 99120442.5 Chinese invention patent application discloses bitter melon seed beverage and working method thereof, this application also just is used for beverages with fairly simple method with bitter melon seed.Research to the active ingredient in the bitter melon seed in the above-mentioned disclosed data does not obviously give enough attention.
Purpose of the present invention is utilized the bitter melon seed in the plant balsam pear exactly, extracts its active ingredient Momordi glucose-reducing polypeptide, is used for prevention and health care to diabetes.
For achieving the above object, the present invention has adopted such measure: Momordi glucose-reducing polypeptide, it is the activeconstituents that from bitter melon seed, extracts, it prepares with following method: bitter melon seed is shelled, clean then and dry, pulverize, use organic solvent degreasing again, alkaline extraction is used in bitter melon seed benevolence powder vacuum-drying after the degreasing again, and this moment is prior to adding pure water in the dry bitter melon seed benevolence powder, the ratio of powder water is 1: 6-10, adding ammoniacal liquor accent pH value is 9.5-9.6, gets supernatant liquor after leaving standstill, and adds acid in supernatant liquor, and the accent pH value is 2.5-3.5, then have precipitation to fold, obtain throw out after the filtration, throw out cleans with pure water, crushed after being dried is called in appropriate amount of purified water and lyophilize.
Taking the present invention of above-mentioned measure, is the active substance that extracts from bitter melon seed, easily absorbed by human body, but Regular Insulin in the human activin reaches the purpose that reduces blood sugar for human body, and the diabetic subject is had certain function of health care.
Below in conjunction with embodiment the present invention is described in further detail:
Get an amount of dry bitter melon seed, the bitter melon seed shell is sloughed decon with special-purpose shelling machine, clean, normal temperature is dry down, bitter melon seed benevolence at freezing and pulverizing below 30 ℃ to less than 40 orders, add doubling dose acetone, soak 6 hours with degreasing, immersion process constantly stirs, left standstill then 1 hour, the taking-up solvent recuperation is utilized, and the bitter melon seed benevolence powder after the first degreasing adds doubling dose acetone soak degreasing, 3-4 time so repeatedly again, obtain the bitter melon seed benevolence powder after the degreasing, in 45 ℃ of following vacuum-drying 6-12 hours, in exsiccant bitter melon seed benevolence powder, add pure water, powder is 1: 8 than water, adding ammoniacal liquor again transfers pH value to 9.5-9.6, continuously stirring was extracted 6 hours, left standstill 1 hour, and it is standby to get supernatant liquor, slag extracts once more as stated above, twice supernatant liquor merges, and adds the vitriol oil to pH value 2.8-3, stirs 1 hour, left standstill 5 hours, filter, get filter residue and clean, be crushed to after the drying at room temperature less than 100 orders with pure water, add appropriate amount of purified water again, carrying out lyophilize to water content is to get final product below 5%.
Claims (4)
1, Momordi glucose-reducing polypeptide, it is characterized in that described Momordi glucose-reducing polypeptide is the activeconstituents that extracts from bitter melon seed, it prepares with following method: bitter melon seed is shelled, clean then and dry, pulverize, use organic solvent degreasing again, the bitter melon seed benevolence powder vacuum-drying after the degreasing, use alkaline extraction again, this moment, the ratio of powder water was 1 prior to adding pure water in the dry bitter melon seed benevolence powder: 6-10, and adding alkali accent pH value is 9.5-9.6, get supernatant liquor after leaving standstill, in supernatant liquor, add acid, and to transfer pH value be 2.5-3.5, then have precipitation to fold, obtain throw out after the filtration, throw out cleans with pure water, and crushed after being dried is called in appropriate amount of purified water and lyophilize.
2, Momordi glucose-reducing polypeptide according to claim 1 is characterized in that described organic solvent is an acetone.
3, Momordi glucose-reducing polypeptide according to claim 1 is characterized in that described alkali is ammoniacal liquor.
4, Momordi glucose-reducing polypeptide according to claim 1 is characterized in that described acid is sulfuric acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02134773 CN1418889A (en) | 2002-09-23 | 2002-09-23 | Momordi glucose-reducing polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02134773 CN1418889A (en) | 2002-09-23 | 2002-09-23 | Momordi glucose-reducing polypeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1418889A true CN1418889A (en) | 2003-05-21 |
Family
ID=4747885
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02134773 Pending CN1418889A (en) | 2002-09-23 | 2002-09-23 | Momordi glucose-reducing polypeptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1418889A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101328221B (en) * | 2008-04-14 | 2011-03-23 | 中国药科大学 | Hypoglycemic polypeptide fused protein, structure and use of derivate thereof |
| CN102002095A (en) * | 2010-05-20 | 2011-04-06 | 中国药科大学 | Microwave irradiation solid phase synthesis of balsam pear hypoglycemic MC-JJ0108 polypeptide analogue and application thereof |
| CN104605136A (en) * | 2015-02-02 | 2015-05-13 | 王�锋 | Preparation method of balsam pear polypeptide |
| CN105613937A (en) * | 2016-03-29 | 2016-06-01 | 北海阿西罗拉生物科技有限公司 | Preparation method of momordica charantia p-polypeptide freeze-dried powder |
| CN107412720A (en) * | 2017-09-15 | 2017-12-01 | 李玉保 | A kind of bitter gourd polypeptide compound plant medicine for treating diabetes and preparation method thereof |
| CN109480054A (en) * | 2018-11-01 | 2019-03-19 | 苏州卫生职业技术学院 | A kind of Polypeptide-k composition and preparation method thereof |
| CN111171112A (en) * | 2020-03-17 | 2020-05-19 | 广西壮族自治区农业科学院 | Enzymatic extraction method of bitter melon seed protein |
-
2002
- 2002-09-23 CN CN 02134773 patent/CN1418889A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101328221B (en) * | 2008-04-14 | 2011-03-23 | 中国药科大学 | Hypoglycemic polypeptide fused protein, structure and use of derivate thereof |
| CN102002095A (en) * | 2010-05-20 | 2011-04-06 | 中国药科大学 | Microwave irradiation solid phase synthesis of balsam pear hypoglycemic MC-JJ0108 polypeptide analogue and application thereof |
| CN104605136A (en) * | 2015-02-02 | 2015-05-13 | 王�锋 | Preparation method of balsam pear polypeptide |
| CN105613937A (en) * | 2016-03-29 | 2016-06-01 | 北海阿西罗拉生物科技有限公司 | Preparation method of momordica charantia p-polypeptide freeze-dried powder |
| CN107412720A (en) * | 2017-09-15 | 2017-12-01 | 李玉保 | A kind of bitter gourd polypeptide compound plant medicine for treating diabetes and preparation method thereof |
| CN109480054A (en) * | 2018-11-01 | 2019-03-19 | 苏州卫生职业技术学院 | A kind of Polypeptide-k composition and preparation method thereof |
| CN111171112A (en) * | 2020-03-17 | 2020-05-19 | 广西壮族自治区农业科学院 | Enzymatic extraction method of bitter melon seed protein |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100391371C (en) | Processing method of pure mulberry freeze dried powder | |
| CN103750328A (en) | Freeze-dried compound berry superfine powder and production method thereof | |
| CN106177339A (en) | A kind of method preparing treatment gout herbal formulation | |
| CN104026253A (en) | Brain-tonifying intelligence-improving health-care sesame oil and preparing method of brain-tonifying intelligence-improving health-care sesame oil | |
| RU2366435C1 (en) | Method for making preparation of natural honey for treatment of various diseases (versions) | |
| CN102210426B (en) | Pollen cream with intestine moisturizing function and preparation method thereof | |
| CN1418889A (en) | Momordi glucose-reducing polypeptide | |
| CN107495357A (en) | The preparation method and product of abalone shells water-soluble extractive and the shell of seaear | |
| CN100413884C (en) | Protein/polypeptide-K obtained from i(momordica charantia) and process for extraction thereof | |
| Shaikh et al. | Hypoglycemic effect of wheatgrass juice in alloxan induced diabetic rats | |
| CN106110294A (en) | A kind of nutritional preparation repairing damaged islet cells and preparation method thereof | |
| CN103251100B (en) | Maggot peptide beverage and production technology thereof | |
| JPH10279496A (en) | Preventive and therapeutic agent for diabetes from rice | |
| JP5794678B2 (en) | Glucagon-like peptide-1 secretion promoter | |
| CN103125798A (en) | Royal jelly, medlar and almond tablet and preparation method thereof | |
| CN1330265C (en) | Bitter gourd juice drink production method | |
| CN108041220A (en) | A kind of weight reducing health protection tea and preparation method thereof | |
| CN1418890A (en) | Process for preparing momordic glucose-reducing polypeptide | |
| JP3283642B2 (en) | Hair restorer from rice | |
| CN113559236A (en) | Selenium-rich walnut and dendrobium composition, tablet and application thereof | |
| US10500242B2 (en) | Method of producing high-nepodin-containing rumex plant extract and high-nepodin-containing rumex plant extract | |
| KR20080032274A (en) | The manufacturing process of an antiaging soap | |
| CN1090488C (en) | Pharmaceutical for treating diabetes, and its prepn. process | |
| CN109349406A (en) | A kind of mango ferment taste peppermint candy and preparation method thereof | |
| KR100391195B1 (en) | a manufacturing technique of a beverage using of an educt which is distilled from Liriope spicata Lour |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Wang Feng Document name: Notice of correction |
|
| ASS | Succession or assignment of patent right |
Owner name: GUANGXI BEISHENG PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: BEIHAI HAIYU AGRICULTURAL DEVELOPMENT CO., LTD. Effective date: 20040924 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20040924 Address after: 536000 No. 168, Beihai Avenue, Beihai, the Guangxi Zhuang Autonomous Region Applicant after: Guangxi Beisheng Pharmaceutical Co. Ltd. Address before: Haiyu District of Haicheng District of Beihai city the Guangxi Zhuang Autonomous Region 88 building 4 floor Applicant before: Beihai Haiyu Agricultural Development Co., Ltd. |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |