CN104605136A - Preparation method of balsam pear polypeptide - Google Patents
Preparation method of balsam pear polypeptide Download PDFInfo
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- CN104605136A CN104605136A CN201510052510.8A CN201510052510A CN104605136A CN 104605136 A CN104605136 A CN 104605136A CN 201510052510 A CN201510052510 A CN 201510052510A CN 104605136 A CN104605136 A CN 104605136A
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- balsam pear
- bitter gourd
- gourd polypeptide
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Abstract
The invention discloses a preparation method of balsam pear polypeptide. The preparation method comprises the following steps of: shelling a balsam pear seed, and screening an unrotten seed kernel; then placing the seed kernel into a crusher for crushing, then screening through a screen of 50-30 meshes, collecting screened materials, and then sterilizing the screen materials by adopting pasteurization; squeezing the screened materials for further deoiling; then carrying out second-step deoiling by utilizing a CO2 extraction method to obtain balsam pear seed powder; placing the balsam pear seed powder into a tank, adding tenfold pure water for mixing and stirring, then adding alkali water, and soaking for 24 hours; then deslagging and dialyzing to extract a supernate; then adding a certain amount of acid to the supernate, and standing for 24 hours; compressing, filtering, collecting and depositing to obtain the balsam pear polypeptide; then freeze-drying the balsam pear polypeptide; then packaging the dried balsam pear polypeptide by using a double-layer food bag, and preserving at low temperature. The balsam pear polypeptide extracted by adopting the process disclosed by the invention is high in nutrient component concentration and more obvious in effect and meets the market demand.
Description
Technical field
The present invention relates to food processing field, particularly a kind of preparation method of bitter gourd polypeptide.
Background technology
Balsam pear has another name called balsam pear, belonging to cucurbitaceous plant, is annual climbing herb, fruit oblong, be yellow time ripe, surface has most irregular strumae, and seed is hidden among Fleshy fruits, and widespread production is in torrid areas, bitter is cold in nature, be a kind of medicine-food two-purpose vegetables, record according to Compendium of Material Medica and " the southern regions of the Yunnan Province book on Chinese herbal medicine ", balsam pear has clearing away heat and removing summer, nourishing blood and invigorating qi, kidney and spleen invigorating, nourishing liver for improving eyesight; Vitamin C content in balsam pear is high, has the effects such as prevention scurvy, Cell protection film, atherosclerosis, raising physical stress ability, cardioprotection; Balsam pear contains trypsin inhibitor, can protease out secreted by inhibition cancer cell, prevents malignant tumour, but also has reducing blood lipid and blood pressure; Balsam pear also has prevention of osteoporosis, regulates endocrine, the effect such as anti-oxidant, simultaneously strengthening body resistance further, improves the reaction mechanism inside health; Research finds, containing fat eliminating element in balsam pear, eats something rare the effect with fat-reducing.
Generally treat diabetes with balsam pear, lipopenicillinase both at home and abroad at present, research has found out Charantin, balsam pear tea, balsam pear powder in succession, but these molecule product volumes are large, are not easily absorbed and utilized by the body; And bitter gourd polypeptide in the past adopts thin-layer chromatography to carry out extraction preparation mostly, but the method extracted amount is few, not easily reclaims, and can not meet the requirement preparing polypeptide in a large number to a great extent.
Summary of the invention
Object of the present invention solves above-mentioned deficiency, and provides a kind of preparation method of bitter gourd polypeptide, solves and extracts balsam pear unicity, meet the demand preparing polypeptide to a great extent.
In order to realize the present invention, the technical scheme provided is:
A preparation method for bitter gourd polypeptide, is characterized in that, comprising:
Step one, shelled by bitter melon seed, screening is without the seed benevolence of rotting; Again seed benevolence is put into after pulverizer is pulverized and cross 50-30 mesh sieve collection screenings, adopt pasteurization to screenings sterilizing afterwards;
Step 2, by squeezing, first step oil removing is carried out to this screenings obtained in step one; Then CO is utilized
2extraction carries out second oil removing, obtains bitter melon seed powder; And,
Step 3, the bitter melon seed powder obtained put in tank add 10 times of pure water mix and blends in step 2, then add buck and invade bubble 24h; After through removing slag and dialyse extraction supernatant; Then in supernatant, add that a certain amount of acid is heavy puts 24h; Namely compression collected by filtration obtains bitter gourd polypeptide.
Preferably, in described step one, described seed benevolence crosses 40 mesh sieves after pulverizing.
Preferably, in described step 2, CO is utilized
2the detailed process that second step oil removing is carried out in extraction comprises: put the screenings through first step oil removing into CO
2in extractor, maintenance extracting pressure is 30MPa-50MPa, and extraction temperature is 10-20 DEG C, extraction 2-3h.
Preferably, in described step 3, the main component of described buck comprises sodium carbonate and potash.
Preferably, in described step 3, described acid pH is 4.0-5.5.
Preferably, in described step 3, it is 25-30h that the supernatant time is extracted in dialysis.
Preferably, also comprise: after described step 3, carry out step 4, described step 4 comprises: the bitter gourd polypeptide obtained in described step 3 is carried out freeze drying; Then by dried bitter gourd polypeptide double-layer food bag packaging, Cord blood is placed in.
Preferably, in described step 4, described freeze drying temperature is 1-3 DEG C.
Beneficial effect of the present invention: the present invention carries out twice oil removing to seed benevolence, adopts CO
2extraction removal is oily, pollution-free, without organic substance residues, product is easily shunted, and extracts seed powder better, reaches the object improving yield and nutritional labeling; In seed powder, add buck, seed powder can be made easily to decompose absorption moisture, keep the original nutritional labeling of balsam pear powder; The present invention adopts the seed benevolence after shelling to combine dialysis extraction as raw material, filter the bitter gourd polypeptide of preparation, and the bitter gourd polypeptide nutritional labeling concentration extracted is high, and molecular weight is large, and the activity of bitter gourd polypeptide is larger, and effect is more obvious.
Detailed description of the invention
Following examples further illustrate of the present invention, but absolutely not limit the scope of the present invention.The present invention is elaborated further referring to embodiment, but those skilled in the art are to be understood that, the present invention is not limited to the preparation method of these embodiments and use, and, those skilled in the art can carry out equivalent replacement, combination, improvement to the present invention according to description of the invention or modify, and these all comprise within the scope of the invention.
Embodiment 1
A preparation method for bitter gourd polypeptide, comprises the following steps: the bitter melon seed choosing 10kg shells, and screening is without the seed benevolence of rotting; Again seed benevolence is put into after pulverizer is pulverized and cross 40 mesh sieves collection screenings, adopt pasteurization to screenings sterilizing afterwards; Screenings is carried out first step oil removing by squeezing; Then CO
2extraction carries out second step oil removing, puts the screenings through first step oil removing into CO
2in extractor, maintenance extracting pressure is 40MP
a, extraction temperature is 15 DEG C, and extraction 2h, all removes oil, obtain bitter melon seed powder; Bitter melon seed powder is put in tank and adds 10 times of pure water mix and blends, then add buck invade bubble 24h; After through remove slag and dialyse 25h extract supernatant; Then in supernatant, add that a certain amount of acid is heavy puts 24h; Namely compression collected by filtration obtains bitter gourd polypeptide, then carries out the freeze drying that temperature is 2 DEG C; By dried bitter gourd polypeptide double-layer food bag packaging, be placed in Cord blood, bitter gourd polypeptide levels is 79.8%.
Embodiment 2
A preparation method for bitter gourd polypeptide, comprises the following steps: the bitter melon seed choosing 10kg shells, and screening is without the seed benevolence of rotting; Again seed benevolence is put into after pulverizer is pulverized and cross 30 mesh sieves collection screenings, adopt pasteurization to screenings sterilizing afterwards; Screenings is carried out first step oil removing by squeezing; Then CO
2extraction carries out second step oil removing, puts the screenings through first step oil removing into CO
2in extractor, maintenance extracting pressure is 30MPa, and extraction temperature is 18 DEG C, and extraction 2h, all removes oil, obtain bitter melon seed powder; Bitter melon seed powder is put in tank and adds 10 times of pure water mix and blends, then add buck invade bubble 24h; After through remove slag and dialyse 28h extract supernatant; Then in supernatant, add that a certain amount of acid is heavy puts 24h; Namely compression collected by filtration obtains bitter gourd polypeptide, then carries out the freeze drying that temperature is 3 DEG C; By dried bitter gourd polypeptide double-layer food bag packaging, be placed in Cord blood, bitter gourd polypeptide levels is 75%.
Embodiment 3
A preparation method for bitter gourd polypeptide, comprises the following steps: the bitter melon seed choosing 10kg shells, and screening is without the seed benevolence of rotting; Again seed benevolence is put into after pulverizer is pulverized and cross 50 mesh sieves collection screenings, adopt pasteurization to screenings sterilizing afterwards; Screenings is carried out first step oil removing by squeezing; Then CO
2extraction carries out second step oil removing, puts the screenings through first step oil removing into CO
2in extractor, maintenance extracting pressure is 50MPa, and extraction temperature is 20 DEG C, and extraction 3h, all removes oil, obtain bitter melon seed powder; Bitter melon seed powder is put in tank and adds 10 times of pure water mix and blends, then add buck invade bubble 24h; After through remove slag and dialyse 30h extract supernatant; Then in supernatant, add that a certain amount of acid is heavy puts 24h; Namely compression collected by filtration obtains bitter gourd polypeptide, then carries out the freeze drying that temperature is 4 DEG C; By dried bitter gourd polypeptide double-layer food bag packaging, be placed in Cord blood, bitter gourd polypeptide levels is 82.8%.
Although embodiment of the present invention are open as above, but it is not restricted to listed in description and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (8)
1. a preparation method for bitter gourd polypeptide, is characterized in that, comprising:
Step one, shelled by bitter melon seed, screening is without the seed benevolence of rotting; Again seed benevolence is put into after pulverizer is pulverized and cross 50-30 mesh sieve collection screenings, adopt pasteurization to screenings sterilizing afterwards;
Step 2, by squeezing, first step oil removing is carried out to this screenings obtained in step one; Then CO is utilized
2extraction carries out second oil removing, obtains bitter melon seed powder; And,
Step 3, the bitter melon seed powder obtained put in tank add 10 times of pure water mix and blends in step 2, then add buck and invade bubble 24h; After through removing slag and dialyse extraction supernatant; Then in supernatant, add that a certain amount of acid is heavy puts 24h; Namely compression collected by filtration obtains bitter gourd polypeptide.
2. the preparation method of bitter gourd polypeptide according to claim 1, is characterized in that, in described step one, described seed benevolence crosses 40 mesh sieves after pulverizing.
3. the preparation method of bitter gourd polypeptide according to claim 1, is characterized in that, in described step 2, utilizes CO
2the detailed process that second step oil removing is carried out in extraction comprises: put the screenings through first step oil removing into CO
2in extractor, maintenance extracting pressure is 30MPa-50MPa, and extraction temperature is 10-20 DEG C, extraction 2-3h.
4. the preparation method of bitter gourd polypeptide according to claim 1, is characterized in that, in described step 3, the main component of described buck comprises sodium carbonate and potash.
5. the preparation method of bitter gourd polypeptide according to claim 1, is characterized in that, in described step 3, described acid pH is 4.0-5.5.
6. the preparation method of bitter gourd polypeptide according to claim 1, is characterized in that, in described step 3, it is 25-30h that the supernatant time is extracted in dialysis.
7. the preparation method of bitter gourd polypeptide according to claim 1, is characterized in that, also comprises: after described step 3, carry out step 4, and described step 4 comprises: the bitter gourd polypeptide obtained in described step 3 is carried out freeze drying; Then by dried bitter gourd polypeptide double-layer food bag packaging, Cord blood is placed in.
8. the preparation method of bitter gourd polypeptide according to claim 7, is characterized in that, in described step 4, described freeze drying temperature is 1-3 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757642A (en) * | 2019-03-21 | 2019-05-17 | 安徽农业大学 | A kind of preparation method of balsam pear solid beverage |
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| CN1315964A (en) * | 1999-04-13 | 2001-10-03 | 普什帕康纳 | Protein/polypeptide-K obtained from i(momordica charantia) and process for extraction thereof |
| CN1418889A (en) * | 2002-09-23 | 2003-05-21 | 北海海玉农业开发有限责任公司 | Momordi glucose-reducing polypeptide |
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| CN1594520A (en) * | 2004-06-22 | 2005-03-16 | 北海安泰生物技术有限公司 | Method for supercritical extraction of balsam pear oil using carbon dioxide |
| CN1858223A (en) * | 2005-04-30 | 2006-11-08 | 邹远东 | Process for preparing bitter gourd polypeptide and use |
| CN101985644A (en) * | 2010-10-25 | 2011-03-16 | 邹远东 | Momordica charantia polypeptide, preparation method and application thereof |
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| 刘维全: "《动物生物化学实验指导》", 31 December 2014, 中国农业出版社 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757642A (en) * | 2019-03-21 | 2019-05-17 | 安徽农业大学 | A kind of preparation method of balsam pear solid beverage |
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Owner name: ZHONGSHI HECHANG BIO-TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: WANG FENG Effective date: 20150805 |
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Effective date of registration: 20150805 Address after: 100025 D0001, room 1, basement, No. 145 Chaoyang North Road, Beijing, Chaoyang District Applicant after: In food and biological science and Technology Co Ltd Address before: 536000 Sichuan Beihai South Road hi tech Industrial Park, room 206, the Guangxi Zhuang Autonomous Region Applicant before: Wang Feng |
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