CN1418890A - Process for preparing momordic glucose-reducing polypeptide - Google Patents
Process for preparing momordic glucose-reducing polypeptide Download PDFInfo
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- CN1418890A CN1418890A CN 02134774 CN02134774A CN1418890A CN 1418890 A CN1418890 A CN 1418890A CN 02134774 CN02134774 CN 02134774 CN 02134774 A CN02134774 A CN 02134774A CN 1418890 A CN1418890 A CN 1418890A
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- melon seed
- bitter melon
- degreasing
- powder
- glucose
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Abstract
The preparation method of momordic glucose-reducing polypeptide uses the bitter melon seed as raw material, uses organic solvent to defat and uses alkali to make extraction, and uses acid to make precipitation so as to obtain the invented product momordic glucose-reducing polypeptide. Its process is simple and yield rate is high.
Description
That the present invention relates to is a kind of preparation method of Momordi glucose-reducing polypeptide.
The active ingredient that contains hypoglycemic in the plant balsam pear, can be used to reduce the blood sugar for human body level, this active ingredient with blood sugar reducing function is a polypeptide class or protein-based, extract this active ingredient to the utilization of active ingredient in the balsam pear and from balsam pear at present so several method is arranged: wherein publication number is 1244531, application number is 9911188.5 Chinese invention patent application, disclosed method is with balsam pear homogenate, stir, suction filtration, transfer pH value, refrigeration, precipitation, leave standstill, then can separate out colourless needle crystal, carry out thin layer chromatography (tlc), the gained crystallization is carried out ply of paper again and is analysed, and obtains Charantin therefrom.And publication number is 1102188, application number is 93119351.6 Chinese invention patent, disclosed is the preparation method who extracts the new component PA of hypoglycemic active protein from the vegetable material balsam pear, has adopted isoelectric precipitation and gel to cross post among its preparation technology, and ultraviolet is picked up the method for surveying fraction collection.Above-mentioned patent application, disclosed all is the method for the active ingredient that extracts from the plant balsam pear, rather than extracts active ingredient from bitter melon seed, and above-mentioned disclosed method complex process, step is various, requires high, relatively be suitable for laboratory middle and small scale preparation, and be unfavorable for industrialized production.
Purpose of the present invention just provides a kind of method of extracting Momordi glucose-reducing polypeptide from bitter melon seed, to overcome the existing deficiency of aforesaid method.
For achieving the above object, the present invention has taked such measure, a kind of preparation method of Momordi glucose-reducing polypeptide, and described Momordi glucose-reducing polypeptide extracts from bitter melon seed, and extracting method is made up of following step:
(1) with the bitter melon seed shelling, shells and stay benevolence, bitter melon seed benevolence is cleaned dry then, pulverize, get only
Bitter melon seed benevolence powder;
(2) with organic solvent with the degreasing of bitter melon seed benevolence powder, or use CO
2The supercritical extraction method degreasing is taken off
Bitter melon seed benevolence powder behind the fat carries out vacuum-drying;
(3) add suitable quantity of water in the bitter melon seed benevolence powder after degreasing, powder is 1 with the ratio of water: 6-10, use
Alkali transfers pH value to carry out alkaline extraction to 9.5-9.6, so extracts twice, collects extracted twice liquid;
(4) said extracted liquid transfers pH value to 2.8-3 with acid, then can separate out precipitation, gets filter residue after the filtration,
The filter residue water cleans, and Air drying is ground into fine powder;
(5) above-mentioned fine powder is called in suitable pure water, in Freeze Drying Equipment lyophilize to water content below 5%,
Can obtain Momordi glucose-reducing polypeptide.
Take the present invention of above-mentioned measure, technology is easy, the yield height, not needing or not specific equipment through complicated cumbersome leaching process yet, preparation cost is low, implement easily, the Momordi glucose-reducing polypeptide with the present invention prepares is easily absorbed by human body, but Regular Insulin in the human activin reaches the purpose that reduces blood sugar for human body.
Below in conjunction with embodiment the present invention is described in further detail:
Get an amount of dry bitter melon seed, with special-purpose shelling machine the bitter melon seed shell is sloughed, decon, clean, normal temperature is dry down, bitter melon seed benevolence obtains less than 40 purpose bitter melon seed benevolence powder at freezing and pulverizing twice below 30 ℃, adds doubling dose acetone in bitter melon seed benevolence powder, soak 6 hours with degreasing, immersion process constantly stirs, and leaves standstill then 1 hour, takes out the solvent recuperation utilization, bitter melon seed benevolence powder after the first degreasing adds doubling dose acetone soak degreasing again, 3-4 time so repeatedly, obtain the bitter melon seed benevolence powder after the degreasing, obtained exsiccant degreasing bitter melon seed benevolence powder in 45 ℃ of following vacuum-drying 6-12 hours; Or with the bitter melon seed benevolence powder CO behind the freezing and pulverizing
2Supercritical extraction method carries out degreasing, obtain exsiccant degreasing bitter melon seed benevolence powder, in exsiccant degreasing bitter melon seed benevolence powder, add pure water, powder is 1: 8 than water, adds ammoniacal liquor again and transfers pH value to 9.5-9.6, and continuously stirring was extracted 6-10 hour, left standstill 1-2 hour, it is standby to get supernatant liquor, and slag extracts once more as stated above, and twice supernatant liquor merges, in supernatant liquor, add the vitriol oil to pH value 2.8-3, stirred 1-2 hour, left standstill 5-7 hour, filter, getting filter residue cleans with pure water, be crushed to after the drying at room temperature less than 100 orders, add appropriate amount of purified water again, carrying out lyophilize to water content is can obtain Momordi glucose-reducing polypeptide below 5%.
Claims (5)
1, a kind of preparation method of Momordi glucose-reducing polypeptide is characterized in that described Momordi glucose-reducing polypeptide extracts from bitter melon seed, extracting method is made up of following step:
(1) with bitter melon seed shelling, shell and stay benevolence, bitter melon seed benevolence is cleaned dry then, pulverize clean bitter melon seed benevolence powder;
(2) with organic solvent with the degreasing of bitter melon seed benevolence powder, or, take off with the degreasing of CO2 supercritical extraction method
Bitter melon seed benevolence powder behind the fat carries out vacuum-drying;
(3) add suitable quantity of water in the bitter melon seed benevolence powder after degreasing, powder is 1 with the ratio of water: 6-10, use
Alkali transfers pH value to carry out alkaline extraction to 9.5-9.6, so extracts twice, collects extracted twice liquid;
(4) said extracted liquid transfers pH value to 2.8-3 with acid, then can separate out precipitation, gets filter residue after the filtration,
The filter residue water cleans, and Air drying is ground into fine powder;
(5) above-mentioned fine powder is called in suitable pure water, in Freeze Drying Equipment lyophilize to water content below 5%,
Can obtain Momordi glucose-reducing polypeptide.
2, method according to claim 1 can be used CO when it is characterized in that the degreasing of bitter melon seed benevolence powder
2The supercritical extraction method degreasing.
3, method according to claim 1 is characterized in that described organic solvent is an acetone.
4, method according to claim 1 is characterized in that described alkali is ammoniacal liquor.
5, method according to claim 1 is characterized in that described acid is sulfuric acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02134774 CN1418890A (en) | 2002-09-23 | 2002-09-23 | Process for preparing momordic glucose-reducing polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02134774 CN1418890A (en) | 2002-09-23 | 2002-09-23 | Process for preparing momordic glucose-reducing polypeptide |
Publications (1)
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| CN1418890A true CN1418890A (en) | 2003-05-21 |
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| CN 02134774 Pending CN1418890A (en) | 2002-09-23 | 2002-09-23 | Process for preparing momordic glucose-reducing polypeptide |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824075A (en) * | 2010-05-20 | 2010-09-08 | 中国药科大学 | Micro-assisted solid-phase synthesis of analogue of MC-JJ0101 polypeptide isolated from Momordica charantia and application of the analogue |
| CN101824073A (en) * | 2010-05-20 | 2010-09-08 | 中国药科大学 | Micro-assisted solid-phase synthesis of analogue of MC-JJ0103 polypeptide isolated from Momordica charantia and application of the analogue |
| CN101824074A (en) * | 2010-05-20 | 2010-09-08 | 中国药科大学 | Micro-assisted solid-phase synthesis of analogue of MC-JJ0107 polypeptide isolated from Momordica charantia and application of the analogue |
| WO2012101657A1 (en) * | 2011-01-28 | 2012-08-02 | Piramal Life Sciences Ltd. | Process for preparation of a herbal extract |
| CN102690321A (en) * | 2012-06-05 | 2012-09-26 | 惠州市鑫福来实业发展有限公司 | Process for extracting protein of cordyceps militaris, and optimization method for optimal extraction conditions of process |
| CN104605136A (en) * | 2015-02-02 | 2015-05-13 | 王�锋 | Preparation method of balsam pear polypeptide |
-
2002
- 2002-09-23 CN CN 02134774 patent/CN1418890A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824075A (en) * | 2010-05-20 | 2010-09-08 | 中国药科大学 | Micro-assisted solid-phase synthesis of analogue of MC-JJ0101 polypeptide isolated from Momordica charantia and application of the analogue |
| CN101824073A (en) * | 2010-05-20 | 2010-09-08 | 中国药科大学 | Micro-assisted solid-phase synthesis of analogue of MC-JJ0103 polypeptide isolated from Momordica charantia and application of the analogue |
| CN101824074A (en) * | 2010-05-20 | 2010-09-08 | 中国药科大学 | Micro-assisted solid-phase synthesis of analogue of MC-JJ0107 polypeptide isolated from Momordica charantia and application of the analogue |
| WO2012101657A1 (en) * | 2011-01-28 | 2012-08-02 | Piramal Life Sciences Ltd. | Process for preparation of a herbal extract |
| CN103458906A (en) * | 2011-01-28 | 2013-12-18 | 皮拉莫企业有限公司 | Process for preparation of a herbal extract |
| US9034400B2 (en) | 2011-01-28 | 2015-05-19 | Piramal Enterprises Limited | Process for preparation of a herbal extract |
| CN103458906B (en) * | 2011-01-28 | 2016-05-25 | 皮拉莫企业有限公司 | Prepare a kind of technique of draft essence |
| CN102690321A (en) * | 2012-06-05 | 2012-09-26 | 惠州市鑫福来实业发展有限公司 | Process for extracting protein of cordyceps militaris, and optimization method for optimal extraction conditions of process |
| CN104605136A (en) * | 2015-02-02 | 2015-05-13 | 王�锋 | Preparation method of balsam pear polypeptide |
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Addressee: Wang Feng Document name: Notice of correction |
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Owner name: GUANGXI BEISHENG PHARMACEUTICAL CO., LTD. Free format text: FORMER OWNER: BEIHAI HAIYU AGRICULTURAL DEVELOPMENT CO., LTD. Effective date: 20040924 |
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Effective date of registration: 20040924 Address after: 536000 No. 168, Beihai Avenue, Beihai, the Guangxi Zhuang Autonomous Region Applicant after: Guangxi Beisheng Pharmaceutical Co. Ltd. Address before: Haiyu District of Haicheng District of Beihai city the Guangxi Zhuang Autonomous Region 88 building 4 floor Applicant before: Beihai Haiyu Agricultural Development Co., Ltd. |
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