CN101348529A - Extraction and separation method of medlar polysaccharide - Google Patents
Extraction and separation method of medlar polysaccharide Download PDFInfo
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- CN101348529A CN101348529A CN 200810212259 CN200810212259A CN101348529A CN 101348529 A CN101348529 A CN 101348529A CN 200810212259 CN200810212259 CN 200810212259 CN 200810212259 A CN200810212259 A CN 200810212259A CN 101348529 A CN101348529 A CN 101348529A
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- matrimony vine
- fructus lycii
- lycium barbarum
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Abstract
The invention provides a method for extracting and separating a polysaccharide of Fructus Lycii. The method comprises the following steps that firstly Fructus Lycii is washed clean, dried, pulverized, and sifted, to obtain a powder of Fructus Lycii with the size of between 40 and 80 meshes; secondly the powder of Fructus Lycii is mixed with distilled water according to a weight ratio of 1:4, and impregnated for 1-2 hours, and then extracted with ultrasonic waves with a power of 150 Watt for 40 to 50 minutes with the temperature of the solution between 55 and 65 DEG C; thirdly the extract from the former step is filtered, the supernatant undergoes decompressed condensation into a volume of 1/3 to 1/2 of the original one, and the condensed liquid is added with ethanol with a concentration of more than 95 percent and a volume of 5 to 8 times that of the condensed liquid, kept stand for 2 to 4 hours at normal temperature to obtain a deposit; and finally, the deposit is frozen, dried and pulverized to obtain the powder of polysaccharide of Fructus Lycii. The extraction procedure is easy to accomplish; compared with the method in prior art, the method provided by the invention lowers the extraction time by two thirds and improves the extraction efficiency for two times.
Description
Technical field
The present invention relates to a kind of extraction and separation method of lycium barbarum polysaccharide, especially by the extraction and separation method of ultrasonic extraction as main means.Belong to technical field of biochemical industry.
Background technology
Wolfberry fruit is the mature fruit of matrimony vine of solanaceae plant, another name FRUCTUS LYCII BARBARI, white thorn, mountain matrimony vine, blood matrimony vine, matrimony vine beans, be called for short matrimony vine, have tonifying kidney and benefiting sperm, nourish the liver to improve visual acuity, blood-enriching tranquillizing, promote the production of body fluid to quench thirst, the effect that moistens the lung and relieve the cough clinically is mainly used in treatment hepatic and renal YIN deficiency, soreness of the waist and knees, has a dizzy spell, diseases such as the many tears of blurred vision and cough due to consumptive disease, have " red treasured " laudatory title, is with a long history, integration of drinking and medicinal herbs, the rare traditional Chinese medicine that has won fame both at home and abroad.Contain abundant lycium barbarum polysaccharide, fat, protein, total free aminoacids, taurine, trimethyl-glycine, VITMAIN B1, B2, E, C in the matrimony vine, in addition, also contain a large amount of mineral elements, as potassium, sodium, calcium, magnesium, iron, copper, manganese, zinc, selenium etc.The record of " obeying hard muscles and bones for a long time " is arranged in the Shennong's Herbal, saying of " nourishing the essence and strengthening QI " arranged in " Mingyi Bielu ", the reputation of " the beneficial people of energy removes consumptive disease " is arranged, in " Shiliao Bencao " so matrimony vine is praised highly by ancient Chinese medicine doctor and dietotherapy scholar.
Modern pharmacological research proves, the contained major ingredient lycium barbarum polysaccharide of matrimony vine is one of its most important functional component, it is a kind of water-soluble polysaccharide, and this polysaccharide is a protein-polysaccharide, and the protein main chain that polysaccharide side chain of being made up of six kinds of monose and 18 seed amino acids are formed constitutes.Lycium barbarum polysaccharide is its main active ingredient of regulating immunity, delaying senility; the immunologic function that can improve the people plays antivirus action indirectly; can directly act on the hypothalamus of human body; promote the adjusting of metabolic function, endocrine function and reproductive function; its prevention to malignant tumour, acquired immune deficiency syndrome (AIDS) also can be played active effect with treatment; it can improve symptoms such as the elderly's fatiguability, poor appetite and blurred vision in addition, and has effects such as hypoglycemic, reducing blood-fat, protection liver, antitumor, anti-ageing, radioprotective.
The traditional technology of extracting lycium barbarum polysaccharide adopts simple water extract-alcohol precipitation method always, and extraction efficiency is low, and active constituent content is not high.Patent 2005100467857 " fermentation extraction of Chinese wolfberry fruit polysaccharide ", disclose a kind of applied bioengineering technology and extracted the method for lycium barbarum polysaccharide, it utilizes the means that consume monose, disaccharide, nitrogenous substances in the Angel Yeast process of growth to improve the purity of lycium barbarum polysaccharide, whole technological process comprises pulverizing, immersion, aerobic fermentation, filtration, decolouring, removal of impurities, vacuum distilling, is prone to fermentation problem such as not exclusively and more than the impurity in this method production process; Patent 021153825 " a kind of lycium barbarum polysaccharide and its production and application ", a kind of method that adopts membrane separation technique to extract lycium barbarum polysaccharide is disclosed, whole technological process comprises steps such as shearing, squeezing, centrifugal, ultrafiltration membrance filter, ultra-filtration membrane concentrate, lyophilize, this method complex process, it is low to produce yield.
Summary of the invention
The objective of the invention is to overcome the deficiency of conventional art, provide a kind of by the extraction and separation method of ultrasonic extraction, to reach the purpose that technology is simple, extraction efficiency is high as the lycium barbarum polysaccharide of main means.
Ultrasonic wave has unique physical property, can impel plant cell tissue's broken wall or distortion, makes in the matrimony vine extracts active ingredients more abundant; But ultrasonic wave also has stronger mechanical shearing effect simultaneously, and effect can make the polysaccharide macro-molecular fracture for a long time, thereby influences its content of effective, and therefore suitably the control ultrasonic time is very necessary.Found following extraction characteristics with 150 watts of ultrasonic oscillation devices by a large amount of experiments: (1) is along with the ultrasonic wave prolongation of action time, the extraction yield of lycium barbarum polysaccharide also raises gradually, the lycium barbarum polysaccharide extraction yield is the highest in the time of 40 to 50 minutes, along with the continuation prolongation of time, the content of lycium barbarum polysaccharide descends to some extent.(2) optimum temps of ultrasonic extraction lycium barbarum polysaccharide is 55 to 65 ℃, and extraction efficiency is higher in this temperature range, and effective constituent can not decomposed.
The concrete steps of lycium barbarum polysaccharide extraction and separation method of the present invention comprise:
(1) pulverizes: after matrimony vine is cleaned oven dry,, obtain 40 to 80 purpose matrimony vine powder through pulverizing, sieving;
(2) soak at room temperature: matrimony vine powder and distilled water were soaked 1～2 hour after 1: 4 the mixed by weight;
(3) supersound extraction: be heated to 55～65 ℃, adjust 150 watts of ultrasonic powers, supersound extraction 40～50 minutes;
(4) concentrate: said extracted liquid is filtered, supernatant liquor is evaporated to 1/3～1/2 of original volume;
(5) alcohol precipitation: add the ethanol more than 95% in concentrated solution, add-on is 5～8 times of concentrated solution volume, and normal temperature left standstill 2～4 hours, separates obtaining precipitation;
(6) drying:, obtain the lycium barbarum polysaccharide powder after the pulverizing with the lyophilize of paste throw out.
The present invention has following remarkable advantage:
A. leaching process is simple, realizes easily;
B. compare with traditional method, extraction time is saved more than 70%;
C. compare with traditional method, extraction efficiency improves more than 200%;
D. lycium barbarum polysaccharide content is higher than traditional method far away in the product powder.
Embodiment
Embodiment 1
(1) pulverizes: after matrimony vine is cleaned oven dry,, collect 40 to 60 purpose matrimony vine powder through pulverizing, sieving;
(2) soak: matrimony vine powder and distilled water soak at room temperature 2 hours after 1: 4 the mixed by weight;
(3) supersound extraction: be heated to 55 ℃, adjust 150 watts of ultrasonic powers, supersound extraction 45 minutes;
(4) concentrate: said extracted liquid is filtered, supernatant liquor is evaporated to 1/3 of original volume;
(5) alcohol precipitation: add the ethanol more than 95% in concentrated solution, add-on is 6 times of concentrated solution volume, and normal temperature left standstill 3 hours, obtains precipitation;
(6) drying:, obtain the lycium barbarum polysaccharide powder after the pulverizing with the lyophilize of paste throw out.
Embodiment 2
(1) pulverizes: after matrimony vine is cleaned oven dry,, obtain 40 to 80 purpose matrimony vine powder through pulverizing, sieving;
(2) soak: matrimony vine powder and distilled water soak at room temperature 1 hour after 1: 4 the mixed by weight;
(3) supersound extraction: be heated to 55 ℃, adjust 150 watts of ultrasonic powers, supersound extraction 40 minutes;
(4) concentrate: said extracted liquid is filtered, supernatant liquor is evaporated to 1/2 of original volume;
(5) alcohol precipitation: add the ethanol more than 95% in concentrated solution, add-on is 5 times of concentrated solution volume, and normal temperature left standstill 2 hours, obtains precipitation;
(6) drying:, obtain the lycium barbarum polysaccharide powder after the pulverizing with the lyophilize of paste throw out.
Embodiment 3
(1) pulverizes: after matrimony vine is cleaned oven dry,, collect 60 to 80 purpose matrimony vine powder through pulverizing, sieving;
(2) soak: matrimony vine powder and distilled water soak at room temperature 2 hours after 1: 4 the mixed by weight;
(3) supersound extraction: be heated to 65 ℃, adjust 150 watts of ultrasonic powers, supersound extraction 50 minutes;
(4) concentrate: said extracted liquid is filtered, supernatant liquor is evaporated to 1/2 of original volume;
(5) alcohol precipitation: add the ethanol more than 95% in concentrated solution, add-on is 8 times of concentrated solution volume, and normal temperature left standstill 4 hours, obtains precipitation;
(6) drying:, obtain the lycium barbarum polysaccharide powder after the pulverizing with the lyophilize of paste throw out.
Claims (3)
1, a kind of extraction and separation method of lycium barbarum polysaccharide is characterized in that this method may further comprise the steps:
(1) pulverizes: after matrimony vine is cleaned oven dry,, obtain 40 to 80 purpose matrimony vine powder through pulverizing, sieving;
(2) soak: matrimony vine powder and distilled water soak at room temperature 1～2 hour after 1: 4 the mixed by weight;
(3) supersound extraction: be heated to 55～65 ℃, adjust 150 watts of ultrasonic powers, supersound extraction 40～50 minutes;
(4) concentrate: said extracted liquid is filtered, supernatant liquor is evaporated to 1/3～1/2 of original volume;
(5) alcohol precipitation: add the ethanol more than 95% in concentrated solution, add-on is 5～8 times of concentrated solution volume, and normal temperature left standstill 2～4 hours, obtains precipitation;
(6) drying:, obtain the lycium barbarum polysaccharide powder after the pulverizing with the lyophilize of paste throw out.
2, the extraction and separation method of lycium barbarum polysaccharide according to claim 1 is characterized in that: in the step (3), the best supersound extraction time is 45 minutes.
3, panaxan's according to claim 1 extraction and separation method is characterized in that: in the step (5), best time of repose is 3 hours.
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| CN2008102122597A CN101348529B (en) | 2008-09-08 | 2008-09-08 | Extraction and separation method of medlar polysaccharide |
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| CN2008102122597A CN101348529B (en) | 2008-09-08 | 2008-09-08 | Extraction and separation method of medlar polysaccharide |
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| CN101348529B CN101348529B (en) | 2010-12-01 |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101851356A (en) * | 2010-06-04 | 2010-10-06 | 北京三浦百草绿色植物制剂有限公司 | Shitake mushroom extract and application thereof |
| CN102872064A (en) * | 2012-09-19 | 2013-01-16 | 宁夏回族自治区药品检验所 | Method for extracting alpha-glucosidase activity inhibitor from lycium barbarum seeds and residues after extraction of lycium barbarum oil from lycium barbarum seeds and application thereof |
| CN102936292A (en) * | 2012-11-22 | 2013-02-20 | 天津科技大学 | Preparation method of lycium barbarum polysaccharide having high antioxidant activity |
| CN103549421A (en) * | 2013-10-24 | 2014-02-05 | 格尔木源鑫堂生物科技有限公司 | Multidimensional quick-acting medlar powder and production method thereof |
| CN103772526A (en) * | 2014-02-21 | 2014-05-07 | 香港理工大学深圳研究院 | Method for efficiently extracting polysaccharide active ingredients in lycium barbarum by using ultrasound enhanced subcritical water |
| CN103788228A (en) * | 2014-02-17 | 2014-05-14 | 银川泰丰生物科技有限公司 | Preparation method of lycium barbarum polysaccharide |
| CN104311692A (en) * | 2014-11-20 | 2015-01-28 | 四川旭华制药有限公司 | Method for extracting polysaccharide substance in rhizophora mucronata through ultrasonic waves |
| CN104642874A (en) * | 2015-03-23 | 2015-05-27 | 哈尔滨工业大学 | Edible fungus composite polysaccharide radiation protection agent and preparation method thereof |
| CN106188322A (en) * | 2016-07-11 | 2016-12-07 | 周晗生 | The extracting method of wolfberry fruit extract |
| CN106177175A (en) * | 2016-08-24 | 2016-12-07 | 张东 | A kind of wolfberry fruit extract, the pharmaceutical preparation and preparation method thereof of antifatigue effect |
| CN106755185A (en) * | 2017-01-05 | 2017-05-31 | 青海康普生物科技股份有限公司 | A kind of microbial method extracts LBP-X industrialized manufacturing technique |
| CN108402269A (en) * | 2018-02-09 | 2018-08-17 | 云南瑞宝生物科技股份有限公司 | Cranberry taste lutein ester sugar-free soft sweet and preparation method thereof |
| CN108517017A (en) * | 2018-04-26 | 2018-09-11 | 许昌学院 | A kind of extracting method of polysaccharides |
| CN110746515A (en) * | 2019-10-28 | 2020-02-04 | 百瑞源枸杞股份有限公司 | Lycium barbarum polysaccharide, lycium barbarum red element and lycium barbarum polypeptide prepared by synchronous separation and preparation method thereof |
| CN113082114A (en) * | 2021-03-12 | 2021-07-09 | 宁夏杞美人生物科技有限公司 | Extraction and preparation process of medlar nutrient |
-
2008
- 2008-09-08 CN CN2008102122597A patent/CN101348529B/en not_active Expired - Fee Related
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101851356A (en) * | 2010-06-04 | 2010-10-06 | 北京三浦百草绿色植物制剂有限公司 | Shitake mushroom extract and application thereof |
| CN102872064A (en) * | 2012-09-19 | 2013-01-16 | 宁夏回族自治区药品检验所 | Method for extracting alpha-glucosidase activity inhibitor from lycium barbarum seeds and residues after extraction of lycium barbarum oil from lycium barbarum seeds and application thereof |
| CN102872064B (en) * | 2012-09-19 | 2015-08-19 | 宁夏回族自治区药品检验所 | Seed of Fructus Lycii and extract method extracting alpha-glucosidase activity inhibitor and uses thereof in the residue after Lycium-seed-oil |
| CN102936292B (en) * | 2012-11-22 | 2015-04-08 | 天津科技大学 | Preparation method of lycium barbarum polysaccharide having high antioxidant activity |
| CN102936292A (en) * | 2012-11-22 | 2013-02-20 | 天津科技大学 | Preparation method of lycium barbarum polysaccharide having high antioxidant activity |
| CN103549421A (en) * | 2013-10-24 | 2014-02-05 | 格尔木源鑫堂生物科技有限公司 | Multidimensional quick-acting medlar powder and production method thereof |
| CN103788228B (en) * | 2014-02-17 | 2016-08-17 | 百瑞源枸杞股份有限公司 | A kind of preparation method of lycium barbarum polysaccharide |
| CN103788228A (en) * | 2014-02-17 | 2014-05-14 | 银川泰丰生物科技有限公司 | Preparation method of lycium barbarum polysaccharide |
| CN103772526A (en) * | 2014-02-21 | 2014-05-07 | 香港理工大学深圳研究院 | Method for efficiently extracting polysaccharide active ingredients in lycium barbarum by using ultrasound enhanced subcritical water |
| CN104311692A (en) * | 2014-11-20 | 2015-01-28 | 四川旭华制药有限公司 | Method for extracting polysaccharide substance in rhizophora mucronata through ultrasonic waves |
| CN104642874A (en) * | 2015-03-23 | 2015-05-27 | 哈尔滨工业大学 | Edible fungus composite polysaccharide radiation protection agent and preparation method thereof |
| CN106188322B (en) * | 2016-07-11 | 2018-11-09 | 周晗生 | The extracting method of wolfberry fruit extract |
| CN106188322A (en) * | 2016-07-11 | 2016-12-07 | 周晗生 | The extracting method of wolfberry fruit extract |
| CN106177175A (en) * | 2016-08-24 | 2016-12-07 | 张东 | A kind of wolfberry fruit extract, the pharmaceutical preparation and preparation method thereof of antifatigue effect |
| CN106755185A (en) * | 2017-01-05 | 2017-05-31 | 青海康普生物科技股份有限公司 | A kind of microbial method extracts LBP-X industrialized manufacturing technique |
| CN108402269A (en) * | 2018-02-09 | 2018-08-17 | 云南瑞宝生物科技股份有限公司 | Cranberry taste lutein ester sugar-free soft sweet and preparation method thereof |
| CN108517017A (en) * | 2018-04-26 | 2018-09-11 | 许昌学院 | A kind of extracting method of polysaccharides |
| CN110746515A (en) * | 2019-10-28 | 2020-02-04 | 百瑞源枸杞股份有限公司 | Lycium barbarum polysaccharide, lycium barbarum red element and lycium barbarum polypeptide prepared by synchronous separation and preparation method thereof |
| CN110746515B (en) * | 2019-10-28 | 2021-08-06 | 百瑞源枸杞股份有限公司 | Lycium barbarum polysaccharide, lycium barbarum red element and lycium barbarum polypeptide prepared by synchronous separation and preparation method thereof |
| CN113082114A (en) * | 2021-03-12 | 2021-07-09 | 宁夏杞美人生物科技有限公司 | Extraction and preparation process of medlar nutrient |
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| CN101348529B (en) | 2010-12-01 |
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