JPH10279496A - Preventive and therapeutic agent for diabetes from rice - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a preventive and therapeutic agent for diabetes from rice which is safe, inexpensive, readily processable and has no fear of adverse effects at all even by regular use for a long period of time. SOLUTION: This preventive and a therapeutic agent for diabetes comprises an extract of rice with any of an acid, an alkali and an organic solvent, a treated solution obtained by treating rice with one or more enzymes of a protease, a cellulolytic enzyme, a lipolytic enzyme and an amylolytic enzyme and a koji and an extract of rice with water under an acidic or an alkali condition. The preventive and the therapeutic agent are obtained by incorporating rice, germinated rice or rice white bran with water, an acid, an alkali or an organic solvent in an amount of 1-100 times as much as that of rice, treating the mixture with one or more enzymes of a protease, a cellulolytic enzyme, a lipolytic enzyme and an amylolytic enzyme and a koji, heating the mixture to 40 deg.C to the boiling temperature and then carrying out alcohol fermentation, lactic acid fermentation, acetic acid fermentation or yeast aerated fermentation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a prophylactic / therapeutic agent for diabetes, which enhances insulin sensitivity and promotes glucose / lipid metabolic activity by using rice.

[0002]

2. Description of the Related Art In the modern age of satiety, excessive intake of calories produces various diseases such as obesity, diabetes, hyperlipidemia, and arteriosclerosis, and the morbidity is steadily increasing.
It is said that even if they do not get sick, there are more people who can be said to be a reserve army. There are two types of diabetes, insulin-dependent diabetes mellitus (type I diabetes) and non-insulin-dependent diabetes mellitus (type II diabetes), with 90% of the latter being the latter type. Non-insulin-dependent diabetes mellitus is an inadequate action of insulin due to overnutrition, such as decreased insulin sensitivity in tissues, insulin resistance, and their symptoms, which increase insulin demand, and eventually burden the pancreas responsible for insulin secretion. It is said that abnormalities in glucose and lipid metabolism due to insufficient secretion are caused. Non-insulin-dependent diabetes mellitus is basically controlled by diet therapy and exercise therapy rather than drug treatment. However, such self-management does not always correct metabolic abnormalities and tends to depend on drugs. However, the drug has problems of side effects due to administration, the amount to be used, and restrictions on the expiration date. In addition, since most of these substances are obtained by mixing unified substances, there is a problem in terms of side effects of the single substance and safety caused by taking the substance for a long time. That is, at present, an effective and safe preventive / therapeutic agent for a disease caused by abnormal sugar / lipid metabolism has not yet been developed.

On the other hand, rice has been developed for use as sake, shochu, mirin, vinegar, koji and the like in addition to the staple food, and has been indispensable to daily life since ancient times. In addition, bran bags are known for cosmetic use. These may be due to the fact that rice was viewed as a staple food only, or at best, as a source of starch. In addition, even though the bran bag is good for the skin, it has only been conventionally used as it is, and there was no concept of an active ingredient, and there was no idea of using the active ingredient at all.

[0004]

There is a risk that the treatment of diabetes using a drug is always accompanied by side effects on the human body. It is known that the use of an oral antihyperglycemic agent, which is a diabetes drug, and insulin injection cause severe hypoglycemia if the action is too strong. Also, there has been no effective way to prevent diabetes other than taking care of food and exercising.
Against this background, there are no side effects,
As a therapeutic agent, a preventive / therapeutic agent for diabetes which is sufficiently safe even when used regularly for a long time is required. An object of the present invention is to provide an agent for preventing and treating diabetes from rice that is safe, inexpensive, and completely safe even when used regularly.

[0005]

Means for Solving the Problems The inventors have been studying various plant components, mainly rice, which is a staple food, from the viewpoint of blending animals and plants. In the process, rice has been discovered to have a number of possibilities and effects that could not be predicted before. Therefore, we have taken up the theme of rice, which is used as a staple food and proved to be the safest, and conducted comprehensive research on rice utilization. As one of the themes, he has been conducting diligent research on preventive and therapeutic agents for diabetes from rice. As a result, it was found that rice contains components that increase insulin sensitivity and promote sugar / lipid metabolism activity, and thus completed the present invention.

[0006] In the present invention, rice or sprouted rice,
Or, the components having the preventive and therapeutic effect of diabetes contained in rice white bran have not been elucidated yet, but rice or germinated rice, or those treated with rice white bran show a preventive and therapeutic effect on diabetes. It has been found. The raw material rice refers to brown rice, white rice, and white bran such as glutinous rice and rice cake, regardless of the quality or type, regardless of japonica or indica rice. Also, sprouted rice is used. In addition, since the active ingredient is stable against heat and light, the above-mentioned raw materials are dipped, steamed, roasted (refer to all kinds of sand roasting, net roasting, hot air roasting, etc.), steamed roasting, and freeze-dried. Surface modification such as UV irradiation, and treatment such as pressurized roasting and frying such as patrice. The rice and the germinated rice are effective as they are, but are preferably ground and used for practical use. In order to pulverize and pulverize the rice and the sprouted rice, a general method using a pulverizer or a rice mill may be used.

[0007] When germinating rice, the germinated rice is immersed in water or sprayed with water to germinate the rice. The temperature at the time of germination is 5 to 70 ° C. However, as long as it germinates,
The temperature and time do not matter. In addition, when there is a risk of water spoiling during germination, it is preferable to replace the water so as not to spoil or perform some preservation. Here, germination refers to everything from immediately before germination to germination.
The sprouted rice is thoroughly washed and used. At this time, it may be dried before use.

In the case of extracting rice with water, the extraction temperature is efficient at a high temperature, but sufficient extraction can be performed even at a low temperature. However, in the case of a low temperature of 40 ° C. or less, the pH is made acidic or alkaline, or a preservative or alcohol is added so as to prevent the rice from spoiling. The extraction time only needs to be able to extract the active ingredient, and may be determined according to the extraction temperature. The extraction may be performed under increased pressure, normal pressure, or reduced pressure. Further, the effective range of the amount of water is 1 to 100 times that of rice. If it is effective, it may be extracted at a lower concentration. However, in this case, there is a possibility that it is necessary to concentrate the extract after the extraction to make the active ingredient dense.

In the case of water extraction, the most problem is the gelatinization phenomenon. If it becomes glue-like, not only does the extraction efficiency deteriorate, but it becomes extremely difficult in actual work. In order to prevent this, the starch may be cut by adding amylase and making it react, or acidifying it with hydrochloric acid or the like. This method can sufficiently solve the problem and has no practical problem at all.
Since the active ingredient in the extract is stable to acids and alkalis, it is effective to perform acid decomposition extraction or alkali decomposition extraction. In this case, neutralization and desalting are performed as necessary.

In the case of extraction with an organic solvent, it is desirable that rice is extracted by pulverizing or powdering as much as possible. The organic solvent may be a general organic solvent such as alcohol, acetone, butanol, n-hexane, methanol, ether and the like, but it is necessary to completely remove the solvent harmful to the human body after extraction.

In the extraction, amylolytic enzymes, proteolytic enzymes, lipolytic enzymes and fibrinolytic enzymes may be used alone or in combination of two or more. In addition, koji may be used. In this case, the koji used may be a commonly used koji, regardless of the type and variety of the koji mold. The enzymatic reaction and the koji may be performed before the extraction, simultaneously with the extraction, or after the extraction. If necessary, sugar may be removed using aeration fermentation with yeast, alcohol precipitation, a synthetic adsorbent, or the like.

In the present invention, alcohol fermentation or lactic acid fermentation is carried out simultaneously with or after each of the above treatments.
Organic acid fermentation such as acetic acid fermentation may be performed. When performing this alcohol fermentation, the extract obtained as described above is saccharified, and the liquid obtained by saccharification or by pressing and filtering is subjected to alcohol fermentation. The enzyme reaction and the alcohol fermentation may be performed simultaneously. That is, saccharification and alcohol fermentation are performed by adding enzymes or koji, and further adding sake or yeast to the rice water. If necessary, sugar fermentation may be performed to carry out alcohol fermentation. Saccharification and alcohol fermentation are 10-2
It is carried out for 4 days, and at this time, if putrefaction is a concern, an acid is added or an appropriate preservative which does not inhibit fermentation is applied.

When alcohol fermentation or aeration fermentation is carried out, there are also advantages such as elimination of stickiness, easy concentration, and easy concentration of the active ingredient. Lactic acid fermentation is performed in the same manner as alcohol fermentation. In this case, lactic acid bacteria are added instead of sake brewer or yeast to perform lactic acid fermentation. Lactic acid fermentation is performed by a common method, and the type of lactic acid bacteria and the conditions of lactic acid fermentation are not limited. Next, in the case of acetic acid fermentation, the fermented product obtained as described above is
Alternatively, after diluting to 4 to 5% alcohol, acetic acid bacteria are added to perform acetic acid fermentation. In addition, in the case where there is no alcohol, acetic acid fermentation may be performed by adding alcohol. Acetic acid fermentation is carried out by a general method, and the type of acetic acid bacteria and the conditions of acetic acid fermentation are not limited.

The processed rice obtained as described above is
Use the residue as it is without separating it, or after pressing and filtering. When used as it is, it is sterilized or disinfected to produce a product. Further, the product may be dried and dried by spray drying or the like. When the product of the present invention is blended, it is made into a dosage form according to a conventional method. The effects of the product of the present invention as an agent for preventing and treating diabetes are shown below based on tests.

[0015] The preventive and therapeutic effects of the product of the present invention on diabetes mellitus are shown by the fact that cultured cells derived from mouse embryos, 3T3-L1 preadipocytes (3T3-Swiss a
lbino cell sub-strain) to promote differentiation into adipocytes (glycerol-
Glycerol uptake was measured as an increase in 3-phosphate dehydrogenase activity, an increase in neutral fat triacylglycerol accumulation), an increase in lipoprotein lipase activity, and an increase in insulin sensitivity. In 3T3-L1 preadipocytes, the activity of enzymes characteristic of adipocytes is hardly detected in fibroblast adipocytes before differentiation, neutral fat is not observed, and hormone sensitivity is not observed. Various enzymes involved in sugar / lipid metabolism by differentiating into fat cells,
The activity of glycerol-3-phosphate dehydrogenase, lipoprotein lipase, hormone-sensitive lipase and the like is increased, the accumulation of neutral fat is observed, and the cells become sensitive to hormones such as insulin and epinephrine. Differentiated adipocytes have almost the same hormonal sensitivity as mature adipocytes in vivo, and are therefore often used for studies on insulin action and the like. As described above, non-insulin-dependent diabetes mellitus is caused by insufficient insulin action due to a decrease in insulin sensitivity and a decrease in glucose / lipid metabolic activity due to insufficient insulin secretion. It is clear from the fact that these symptoms are not caused by suppression of adipocyte differentiation because diabetes is likely to occur in obese people. Fat cells accumulate neutral fat, but the amount that can accumulate is also limited, and even if food is exceeded, excessive intake of food increases the number of fat cells. Therefore, in an obese person, each fat cell is enlarged, and the number of fat cells will be large. However, such increased fat cells are not always functioning normally, but rather the opposite. Insulin is required to metabolize calories ingested through meals, because insulin has the effect of taking up a large amount of sugar into cells. Hypertrophic fat cells become less sensitive to insulin and require more insulin. The more calories you consume, the more insulin you need. If such a state continues, the pancreas, which is an insulin-secreting organ, becomes fatigued, cannot sufficiently secrete insulin, and develops diabetes. Therefore, it is important not only to promote adipocyte differentiation but also to promote the differentiation of adipocytes having normal metabolic activity.

(Experimental method 1) Culture method: 3T3-L1 preadipocytes were prepared from DME medium (Dulbecos M) containing 10% fetal bovine serum (FBS).
5 using modified Eagle Medium.
After culturing at 37 ° C. in% carbon dioxide, the medium was replaced every 2 to 3 days, and after reaching confluence, a differentiation inducing treatment was performed. That is, the product of the present invention was added to the medium, and 10 μg / ml
Culture was performed in the presence of insulin. The addition amount is 33 μl (3.3%) per 1 ml of the culture medium in the case of a liquid, and 1% solution in physiological saline in the case of a solid.
3 μl (300 μg / ml) was added. After reaching confluence, the medium was exchanged five times, and then the culture was stopped and glycerol-3-phosphate dehydrogenase (GP
DH) activity, the amount of neutral fat (triacylglycerol: TG) accumulated in cells, lipoprotein lipase activity, and glucose uptake were measured by the following methods. The culture was performed using a 6-well plate in a group of 3 wells. As a control, those cultured in a normal medium even after reaching confluence (BASAL), 10 μg / m
l) Insulin was added, and as a positive control, the cells were cultured for 2 days in a medium supplemented with 0.25 μM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, and 6 μg / ml insulin, and then returned to a normal medium again. (DMI)
Were also measured.

(Experimental method 2) GPDH activity measuring method: After stopping the culture, the cells were washed twice with physiological saline, and 25 mM Trs-HCl 1 mM EDT.
1 ml of Abuffer (pH 7.4) is added, the cells are detached, and the cells are sonicated under ice-cooling. This cell solution is transferred to 500r
After centrifugation at 4 ° C. for 10 minutes at pm, a portion other than the supernatant and the precipitate is collected and used as a sample for GPDH activity and protein measurement. The GPDH activity is determined from the absorbance at a wavelength of 340 nm from the absorbance at a wavelength of 340 nm. It was shown as a value divided by the amount of protein measured by the method.

(Experimental Method 3) Method for measuring TG accumulation: After the culture was stopped, the cells were washed twice with physiological saline, and 25 mM Trs-HCl 1 mM EDT.
Abuffer (pH 7.4) is added to detach the cells, and the cells are sonicated in ice. Lipoprotein lipase, glycerol kinase, glycerol-3-phosphate oxidase, peroxidase, ATP, and 4-aminoantipyrine were added to the cell solution and reacted at 37 ° C. for 10 minutes.
The absorbance at 505 nm is measured.

Method for measuring lipoprotein lipase (LPL activity) activity: After stopping the culture, the cells were washed twice with physiological saline, and 50 mM NH ₄ Cl—NH ₄ OH 20 mg / m 2.
The cells are detached by adding IBSA buffer (pH 8.2), and sonicated in ice-cooling. 1500 rpm, 4 ° C
In centrifugation for 10 min, the supernatant, the portion other than the precipitate was collected, ^{3 H} label triolein emulsion, rat serum, 0.
2MTrs-HCl 3% BSA buffer (pH
8.2) After adding the mixture and incubating for 1 hour, methanol: chloroform: heptane (1.41: 1.
25: 1 :) The mixture was quenched by adding a mixed solution, pH 11 potassium buffer was added, and the mixture was strongly vortexed.
After centrifugation at 500 rpm for 10 minutes, an appropriate amount of acetic acid is added to the supernatant, and then a scintillator (ACS-II) is added to measure the radioactivity.

(Experimental Method 4) Method of measuring glucose uptake: Insulin sensitivity was indicated by glucose uptake. Since the uptake of sugar by insulin occurs very quickly, considering the measurement error, the amount of glucose was not directly measured, but the neutral fat synthesized by the glucose was extracted and taken as the amount of uptake. That is, after the culture is stopped, the cells are washed twice with a physiological saline, and the cells are washed with 0.5% Hanks buffer 1m.
Add 1 / well. 20 μL of 1 μM insulin (a
After adding 20 μL of physiological saline (without insulin b) and preincubating at 37 ° C. for 10 minutes, 50 μL of ¹⁴ C-labeled glucose was added.
Add L and shake lightly. After incubating again at 37 ° C. for 30 minutes, the buffer and the like added previously are discarded, and alkaline water is added to detach the cells. An extraction reagent is added to the detached cells, vortex is stronger, and Heptan is added, and vortex is stronger. At room temperature, 2000 rpm,
Centrifuge for 5 minutes, take the supernatant into a vial, add toluene scintillator, and measure the radioactivity.

GPDH activity, TG accumulation, LPL activity,
The results of the glucose uptake measurement are shown in Tables 1 to 3.

[0022]

[Table 1]

[0023]

[Table 2]

[0024]

[Table 3]

The product of the present invention increased the activity of glycerol-3-phosphate dehydrogenase and increased the accumulation of triacylglycerol in this experiment. GPDH was a control untreated group (Ba
sal) has almost no activity, and the activity increases with the addition of insulin. A mixture of dexamethasone, 3-isobutyl-1-methylxanthine, and insulin, which are common as potent differentiation promoters, greatly enhanced the activity, but the product of the present invention enhanced the enzyme activity to be equal to or more than that. . In addition, the amount of triacylglycerol accumulated was also GP
It showed the same tendency as DH activity, and the product of the present invention promoted adipocyte differentiation. Lipoprotein lipase is an enzyme that breaks down neutral fat in the blood rather than in fat cells,
Thus, fat cells have a significant effect on the metabolism of other tissues. Since the product of the present invention also increased the activity of this enzyme, it was found that the metabolism of adipose tissue and other tissues was also promoted. In addition, the product of the present invention increases the amount of glucose uptake, and the insulin treatment before the measurement of the amount of uptake significantly increases the amount of uptake, indicating an increase in insulin sensitivity.

Adipose tissue is often considered to be an inactive organ because it has a strong image as an energy storage organ that stores energy as neutral fat, but actually synthesizes and degrades neutral fat, It is a metabolically active organ that secretes bioactive substances. If the adipose tissue does not function properly, metabolism in the whole body cannot be performed smoothly as in diabetes, which exhibits insulin resistance and decreases glucose uptake in the tissue. Adipose tissue is an important target cell of insulin, and normal fat cells are necessary for normal insulin action in the whole body. Like the 3T3L1 preadipocytes used in this experiment,
The activity of enzymes such as GPDH and LPL is hardly detected due to undifferentiation, and a large number of preadipocytes having low sugar and lipid metabolic activity having no sensitivity to hormones such as insulin are present in actual living bodies. It is well known that a therapeutic agent for diabetes such as a thiazolidine derivative has an action of promoting differentiation into adipocytes, but the product of the present invention has a similar action. It is considered that the product of the present invention activates glucose and lipid metabolism in vivo by differentiating preadipocytes having low metabolic activity into normal adipocytes and increasing sensitivity to insulin.

[0027]

【Example】

(Example 1) Brown rice is crushed with a grinder, and the crushed brown rice 500
g was obtained. 10 g of amylolytic enzyme and 150 g of water
0 ml was added. After that, gradually increase the temperature,
After boiling extraction for 5 minutes, the mixture was cooled. Then, it was pressed and filtered to obtain 1420 ml of the product of the present invention and 560 g of residue. (Example 2) Pulverized rice 500 is crushed with a pulverizer,
g was obtained. 2 g of protease, 2 g of lipolytic enzyme, 2 g of fibrinolytic enzyme, 2 g of amylolytic enzyme and 15 g of water
After adding 00 ml, the mixture was left at 50 ° C. for 5 hours, boiled and cooled. Then, it was pressed and filtered to obtain 1420 ml of the product of the present invention and 560 g of residue.

Example 3 In the same manner as in Example 1, 2,000 g of rice extract was obtained. Koji and yeast were added to this extract, and alcohol fermentation was performed for 16 days. Then, it was squeezed and filtered to obtain 1880 ml of the product of the present invention and 80 g of a residue. (Example 4) 2000 ml of the product of the present invention obtained in Example 1
After adding 2 g of saccharifying enzyme and reacting for 15 hours, sterilized by boiling, cooled to 37 ° C., added with 200 ml of a starter in which lactic acid bacteria were previously cultured, sealed well with stirring,
Lactic acid fermentation was performed at 37 ° C. for 2 days. Then, it was pressed and filtered to obtain 1380 ml of the product of the present invention and 590 g of a residue.

(Example 5) To 1000 ml of the product of the present invention obtained in Example 1, 80 ml of 95% ethanol was added.
Acetic acid fermentation was performed for 0 days. Thereafter, the mixture was filtered to obtain 990 ml of the product of the present invention. (Example 6) 2000 ml of the product of the present invention obtained in Example 1
Then, 3 g of a saccharifying enzyme was added thereto, and the mixture was reacted for 15 hours. Then, yeast was added, and aeration fermentation was performed for 24 hours to remove sugar. Thereafter, the mixture was filtered to obtain 1890 ml of the product of the present invention.

(Example 7) White rice of Indica rice was crushed with a grinder to obtain 500 g of white rice of Indica rice.
1500 ml of water was added to the pulverized product, the pH was lowered with HCl, and the mixture was left for 10 days. Thereafter, the product was pressed and filtered to obtain 1200 ml of the product of the present invention and 760 g of a residue. (Example 8) 2N-NaOH 1500m in 500g of Shiranuka
1 was added and left for 5 days. Then squeeze, filter,
1300 ml of a clarified liquid and 700 g of a residue were obtained. The clarified solution was neutralized with 10N-HCl to obtain 1580 ml of the product of the present invention.

Example 9 White rice of glutinous rice was crushed with a grinder to obtain 500 g of white rice of glutinous rice. 1500 ml of 40% ethanol was added to the pulverized product, and the mixture was left for 5 days. Then, it was squeezed and filtered to obtain 1300 ml of a clear liquid and 650 g of a residue. 2000 ml of water was added to the clarified solution, and the mixture was concentrated by a rotary evaporator.
00 ml were obtained. Example 10 Starch-degrading enzyme 1 in 500 g of indica rice
0 g and 1500 ml of water were added. Thereafter, the temperature was gradually increased, and after boiling extraction for 5 minutes, the mixture was cooled. Then, it is squeezed and filtered.
g was obtained.

Example 11 To 500 g of white bran were added 2 g of protease, 2 g of lipolytic enzyme, 2 g of fibrinolytic enzyme, 2 g of starch-degrading enzyme and 1500 ml of water, left at 50 ° C. for 5 hours, boiled and cooled. Then, it was squeezed and filtered to obtain 1480 ml of the product of the present invention and 500 g of a residue. (Example 12) In the same manner as in Example 10, rice extract 2
000 g were obtained. Add koji and yeast to this extract and add 16
Alcohol fermented for days. Then, it was squeezed and filtered to obtain 1860 ml of the product of the present invention and 80 g of a residue.

Example 13 To 2000 ml of the product of the present invention obtained in Example 10, 2 g of saccharifying enzyme was added, reacted for 15 hours, sterilized by boiling, cooled to 37 ° C., and lactic acid bacteria were cultured in advance. After adding 200 ml of starter,
The mixture was sealed well with stirring, and lactic acid fermentation was performed at 37 ° C. for 3 days. Then, it is squeezed and filtered, and 1370 ml of the product of the present invention and residue 60
0 g was obtained. (Example 14) Inventive product 1000 obtained in Example 10
80 ml of 95% ethanol was added to each ml, and acetic acid fermentation was performed for 20 days. Thereafter, the mixture was filtered and the product of the present invention 970m
1 was obtained.

(Example 15) To 2000 ml of the product of the present invention obtained in Example 10, 1 g of a saccharifying enzyme was added, and the mixture was reacted for 15 hours. Then, yeast was added, followed by aeration fermentation for 24 hours to remove sugar. Thereafter, the mixture was filtered to obtain 1900 ml of the product of the present invention. (Example 16) 1500 ml of water was added to 500 g of glutinous rice, the pH was increased with NaOH, and the mixture was allowed to stand for 10 days. Thereafter, the resultant was squeezed and filtered, and the obtained clear solution was neutralized to obtain 1250 ml of the product of the present invention and 710 g of a residue.

Example 17 2 in 500 g of Japonica rice
1500 ml of N-HCl was added and left for 5 days. Then, it is pressed and filtered, and 1350 ml of the clarified liquid and 650 residues
g was obtained. This clarified solution is neutralized with 10N-NaOH,
1560 ml of the product of the present invention was obtained. The product of the present invention obtained in the above examples is adjusted to an appropriate form and used as a trial. Examples in which the product of the present invention is blended are described below. In addition, the composition examples are not limited to the following examples.

Example 18 Tablets 100 g of the product of the present invention obtained in Example 10 was dried by freeze drying to obtain 20 g of a dried product. This dried product 1
0 g was obtained as follows. Invention product 10.0 g Polyethylene glycol 6000 10.0 g Sodium lauryl sulfate 1.5 g Corn starch 3.0 g Lactose 25.0 g Magnesium stearate 0.5 g After weighing the above components, polyethylene glycol 600
0 is heated to 70 to 80 ° C, the product of the present invention, sodium lauryl sulfate, corn starch and lactose are added thereto, mixed, and then cooled as it is. The solidified mixture is granulated in a grinder. The granules are mixed with magnesium stearate and then compressed and compressed into tablets weighing 250 mg.

(Example 19) Soft drink The present invention product obtained in Example 2 15.0% (weight ratio) Licorice extract 0.01% Sugar 4.0% Purified water 78.49% Lemon juice 2.5 % Or more of the blended materials were mixed and dissolved by a conventional method to obtain a soft drink.

Example 20 Powder 10 kg of dextrin according to the present invention obtained in Example 10 and 6 kg of dextrin were mixed, and the mixture was dried by spray drying to obtain 7 kg of powder.

[0039]

According to the present invention, diet therapy, exercise therapy,
A prophylactic / therapeutic agent for diabetes which can easily and safely exhibit insulin sensitivity increase and glucose / lipid metabolic activity promoting effect can be obtained irrespective of drug therapy. Since rice was a staple food, its production methods and uses in new fields other than food were hardly developed. In addition, rice has been eaten as a staple food and other food ingredients, and its safety has been well proven. In other words, it is extremely significant that the present invention not only finds an excellent diabetes preventive / therapeutic agent, but also finds new uses of rice, and can increase consumption by improving the image of rice. .

Claims (13)

[Claims] 1. An agent for preventing or treating diabetes comprising an aqueous extract of rice or a processed product of the aqueous extract. 2. The method of claim 1, wherein the treatment of the rice water extract comprises the step of:
The diabetes preventive / therapeutic agent according to claim 1, wherein the agent is treated with one or two or more enzymes selected from the group consisting of a fibrinolytic enzyme, a lipolytic enzyme, a starch-degrading enzyme and koji. 3. The preventive / therapeutic agent for diabetes according to claim 1, wherein the treatment of the water extract of rice is a treatment carried out under acid or alkaline conditions. 4. Rice or germinated rice or rice white bran is added with water in an amount of 1 to 100 times the amount of rice, and comprises a protease, a fibrinolytic enzyme, a lipolytic enzyme, a starch-degrading enzyme, and a koji. A method for producing a diabetic preventive / therapeutic agent comprising heating one or more enzymes selected from the group and then heating the mixture to a temperature of from 40 ° C. to boiling. 5. A rice or sprouted rice or rice white bran is added with water in an amount of 1 to 100 times the amount of rice, and is composed of a protease, a fibrinolytic enzyme, a lipolytic enzyme, a starch-degrading enzyme, and a koji. After the action of one or more enzymes selected from the group, the mixture is heated to 40 ° C. to a boiling temperature, and then alcoholic fermentation, lactic acid fermentation, acetic acid fermentation, or yeast aeration fermentation is performed. A method for producing the agent for preventing or treating diabetes according to claim 4. 6. A preventive / therapeutic agent for diabetes comprising an acid or alkali extract of rice or a processed product of the acid or alkali extract. 7. The treatment of an acid or alkali extract of rice comprises:
7. Diabetes prevention / treatment according to claim 6, wherein the treatment is carried out with one or more enzymes selected from the group consisting of proteolytic enzymes, fibrinolytic enzymes, lipolytic enzymes, amylolytic enzymes and koji.
Therapeutic agent. 8. Rice or germinated rice or rice white bran is added with an acid or alkali in an amount of 1 to 100 times the amount of rice to produce a protease, fibrinolytic enzyme, lipolytic enzyme, amylolytic enzyme and koji. A method for producing a prophylactic / therapeutic agent for diabetes, which comprises heating one or more enzymes selected from the group consisting of: 9. Rice or germinated rice or rice white bran is added with an acid or alkali in an amount of 1 to 100 times the amount of rice to produce a protease, a fibrinolytic enzyme, a lipolytic enzyme, an amylolytic enzyme, and a koji. After reacting one or more enzymes selected from the group consisting of: heating the mixture to a temperature of 40 ° C. to boiling, and then performing alcohol fermentation, lactic acid fermentation, acetic acid fermentation, or yeast aeration fermentation. 9. A method for producing the agent for preventing or treating diabetes according to claim 8, comprising: 10. A prophylactic / therapeutic agent for diabetes comprising an organic solvent extract of rice or a processed product of the organic solvent extract. 11. The treatment of the organic solvent extract of rice is treatment with one or more enzymes selected from the group consisting of a protease, a fibrinolytic enzyme, a lipolytic enzyme, a starch degrading enzyme, and a koji. The agent for preventing or treating diabetes according to claim 10. 12. To rice or sprouted rice or rice white bran, an organic solvent in an amount of 1 to 100 times the amount of rice is added, and a protease, fibrinolytic enzyme, lipolytic enzyme, amylolytic enzyme and koji are added. A method for producing a prophylactic / therapeutic agent for diabetes comprising heating one or more enzymes selected from the group and then heating the mixture to a temperature of from 40 ° C. to boiling. 13. A method in which rice, germinated rice, or rice white bran is added with an organic solvent in an amount of 1 to 100 times the amount of rice to produce a protease, fibrinolytic enzyme, lipolytic enzyme, amylolytic enzyme, and koji. After allowing one or more enzymes selected from the group to act, heating the mixture to a temperature of 40 ° C. to boiling and then performing alcohol fermentation, or lactic acid fermentation, or acetic acid fermentation, or yeast aeration fermentation. A method for producing a prophylactic / therapeutic agent for diabetes according to claim 12.