JP3779736B2

JP3779736B2 - Antihyperlipidemic agent from rice

Info

Publication number: JP3779736B2
Application number: JP31109093A
Authority: JP
Inventors: 孝徳山
Original assignee: 株式会社創研
Priority date: 1993-11-18
Filing date: 1993-11-18
Publication date: 2006-05-31
Anticipated expiration: 2021-05-31
Also published as: JPH07138176A

Description

【０００１】
【産業上の利用分野】
本発明は、米または発芽させた米を原料として得られる医薬、食品等の分野で使用可能な抗高脂血症剤に関するものである。
【０００２】
【従来の技術】
日本人の死亡原因は、癌、心疾患、脳血管疾患の順になっているが、心疾患と脳血管疾患の合計では、圧倒的に癌より多くなっている。したがって、動脈硬化予防は健康な生活を送る上で最も重要な課題となっている。
動脈硬化、特に狭心症や心筋梗塞の原因となる因子としては、高血圧、喫煙、高脂血症が三大主因子とされている。高血圧は副作用の少ない降圧剤が登場し、そのコントロールが容易となってきたが、高脂血症については、食生活の管理が最も大事とされているのが、現状である。
例えば、食事からとる脂肪（特にコレステロール）と虚血性心疾患との関係は、既に明らかとなっていて、アメリカでは約２０年前から、食生活の見直しを始め、その改善によって血液中のコレステロール値を下げるキャンペーンが展開された。
【０００３】
【発明が解決しようとする課題】
安全が実証されている食物で、血液中の脂質成分の過剰の蓄積（すなわち、高脂血症）を抑制するものがあれば、動脈硬化の予防になり、その開発が待たれている。
本発明は、安全で安価であって、原料供給が安定しており、加工が容易で、長期に亘って常用しても全く安全な抗高脂血症剤を提供することを目的とするものである。
【０００４】
【課題を解決するための手段】
本発明者らは、動植物合和すの観点から、主食である米を中心に種々の植物成分の研究を進めてきた。その過程で、米には今まで予測できなかった数多くの可能性および効果があることが判明してきた。そこで、主食として用いられ、安全性が最も高いことが実証されている米をテーマとして取り上げ、米の総合利用研究を行ってきた。そのうちの一つのテーマとして、米からの抗高脂血症剤について鋭意研究を重ねてきたのであるが、その過程で、米および発芽させた米には抗高脂血症剤としての効果を有する成分が含有されていることを見出し、本発明を完成するに至った。
即ち、本発明（１）は、白米の粉砕物を有効成分として含有する抗高脂血症剤である。
本発明（２）は、白米の抽出物を有効成分として含有する抗高脂血症剤である。
本発明（３）は、白米の加水物を酵素分解または麹を作用させたものを有効成分として含有する抗高脂血症剤である。
本発明（４）は、白米を抽出するに当たり、その抽出前、抽出と同時または抽出後に酵素分解または麹を作用させたものを有効成分として含有する抗高脂血症剤である。
本発明（５）は、白米の抽出物あるいは酵素分解または麹を作用させたものに、アルコール発酵あるいは有機酸発酵を行ったものを有効成分として含有する抗高脂血症剤である。
本発明（６）は、白米を、白米、玄米または発芽米の形態で用いる、前記発明（１）〜（５）のいずれか一つの抗高脂血症剤である。
本発明（７）は、抗動脈硬化のための、前記発明（１）〜（６）のいずれか一つの抗高脂血症剤である。
【０００５】
本発明において、米および発芽させた米に含有されている抗高脂血症剤としての効果を有する成分は、未だ解明するに至っていないが、米および発芽させた米を、下記のように処理したものは、抗高脂血症剤としての効果を示すことが判明した。
▲１▼ 発芽させた米の粉砕物をそのまま、あるいはこれを含有してなるもの。
▲２▼ 米または発芽させた米の抽出物をそのまま、あるいはこれを含有してなるもの。
▲３▼ 米および発芽させた米の加水物に酵素分解または麹を作用させたものをそのまま、あるいはこれを含有してなるもの。
▲４▼ 米または発芽させた米を抽出するに当たり、その抽出前、抽出と同時または抽出後に酵素分解または麹を作用させたものをそのまま、あるいはこれを含有してなるもの。
▲５▼ 米または発芽させた米の抽出物あるいは酵素分解または麹を作用させたものに、アルコール発酵あるいは有機酸発酵を行なったものをそのまま、あるいはこれを含有してなるもの。
【０００６】
本発明で使用される米とは、ジャポニカ，インディカ米を問わず、うるち米、および餅米等の玄米および白米を指し、品種、種類は問わない。さらに、精白時に出てくる９２％以上の赤糠、あるいは９２％以下の白糠を使用してもよく、安価で経済的である。また、発芽させた米が使用される。なお、有効成分は、熱および光に対して安定であるため、上記の原料は、浸漬、蒸煮、焙煎（砂焙り、網焙り、熱風焙煎等全てを指す）、蒸煮焙煎、凍結乾燥等の表面変性、ＵＶ照射等の光変性、パットライス等の加圧焙煎、揚げる等の原料処理をしてもよく、また、効果も変わらなかった。
米および発芽させた米は、そのまま用いても有効であるが、実用上の面から粉砕して用いるのが好ましい。米および発芽させた米を粉砕して粉体化するには、粉砕機または精米機を用い一般的な方法で行なえばよい。
【０００７】
米を発芽させる場合、胚芽のついた米を水に浸漬あるいは水を噴霧して発芽させる。発芽させる時の温度は５〜７０℃である。ただし、発芽さえすれば、温度および時間は問わない。また、発芽中に水が腐敗する危険性がある場合は、腐敗しないように水を取り替えるか、何らかの防腐を行うのが好ましい。ここで、発芽とは、発芽する直前から発芽したものまで全てをさす。この発芽させた米をよく洗浄して用いる。この時、乾燥して用いてもよい。
米または発芽させた米を抽出、あるいは酵素分解または麹を作用させる場合、原料の米を粉砕して顆粒あるいは粉体化すると、表面積が大きくなるため効率がよくなる。粉砕しなくてもよいが、この場合には、米組織の分解および抽出に長時間を要する。
【０００８】
米または発芽させた米を水抽出する場合、抽出温度は、高温が効率的であるが、低温でも十分に抽出を行うことができる。ただし、４０℃以下の低温の場合は、ｐＨを酸性あるいはアルカリ性にするか、防腐剤あるいはアルコールを加えて、米が腐敗しないように処理することが望ましい。抽出時間は、有効成分さえ抽出できれば、長くても短くてもよく、抽出温度、抽出時間により定めればよい。また、抽出は、加圧下または常圧下で行っても、減圧下で行ってもよい。
水抽出の場合、最も問題になるのは糊化現象である。糊状になれば、抽出効率が悪くなるばかりでなく、実作業においては困難を極める。これを防ぐためには、アミラーゼを加えて反応させるか、塩酸などで酸性にして澱粉を切ってやればよく、この方法を用いることにより、十分に解決でき、実用上も全く問題はない。
【０００９】
抽出物中の有効成分は、酸，アルカリに安定であるためか、酸分解抽出、あるいはアルカリ分解抽出を行うのも有効である。この場合、必要により中和、脱塩を行う。
有機溶媒で抽出する場合も、米はなるべく微粉砕または粉体化して抽出することが望ましい。有機溶媒はアルコール，アセトン，ｎ−ヘキサン，メタノール等の一般的な有機溶媒でよいが、人体に対して有害なものは抽出後、溶媒を完全に除去する必要があるので安全なものがよい。
また、米あるいは発芽させた米を酵素分解、または麹を作用させてもよい。ここで言う酵素分解とは、澱粉分解酵素，蛋白分解酵素，脂肪分解酵素，繊維分解酵素，リグニン分解酵素，ペクチン分解酵素等米に働く酵素を１種または２種以上作用させることをいう。また、麹として麹菌の種類および米の品種，種類は問わない。
【００１０】
さらに、前記の抽出を行うに当り、抽出の前、抽出と同時または抽出の後に、上記の酵素分解および麹を作用させてもよい。
本発明においては、さらに上記の処理を行なうと同時または処理後、アルコール発酵あるいは乳酸発酵、酢酸発酵等の有機酸発酵を行うと、次のような点で有効である。
まず、アルコール発酵を行なえば、濃縮がしやすく、有効成分の濃縮が容易になる。また、乳酸発酵は飲料等の用途に使用する場合、風味をよくし、酢酸発酵は酢という調味液用途として本発明品を利用することができ、有機酸発酵することにより幅広い用途として使用することができる。
また、９２％以上の赤糠部分を調べてみたところ、効果はあるが、弱いことが判明した。
【００１１】
以上のようにして得られた本発明品は、残渣を分離することなくそのまま、あるいは圧搾、濾過して用いる。また、そのまま用いるときは、殺菌あるいは除菌して用いる。乾燥して粉体、顆粒、錠剤等にして用いてもよい。さらに、様々な食品に配合して用いることもできる。
以下、具体的に本発明品の抗高脂血症効果について調べた結果を記載する。
４週齢のｄｄＹ系雄性マウスを、室温２５℃、湿度６０％に保たれた動物室で１週間、および水を自由摂取させて飼育した後、実験に供した。実験は１群１０匹で行った。被験液は１日１回午前１０時に１群当たり２０ｍｌを給水瓶に入れ、自由に摂取させた。投与４週間後に、エーテル麻酔下頚動脈より全血採血し、定量操作に必要な処理をした後、血液成分の分析を行った。
その結果を表１に示す。
【００１２】
【表１】

【００１３】
表１に示すように、本発明品投与群において、血中ＶＬＤＬ濃度がいずれも有意に減少した。ＨＤＬ−コレステロール値は、本発明品投与群において上昇した。ＨＤＬ−コレステロールはコレステロールを末梢組織から肝臓に転送する働きがあり、細胞内コレステロールの除去作用を担っている。血中のトリグリセライドおよび総脂質は、本発明品投与群において減少を示した。
以上の結果は、本発明品が明らかに血液中の脂質成分を減少させる作用を有していることを示している。したがって、本発明品は、高脂血症の発症予防に極めて有効であることが判明した。
【００１４】
【実施例】
（実施例１）
胚芽のついたままの米１ｋｇを２５℃の水につけ、３日間浸漬させ、米を発芽させた。この発芽米をよく洗浄した後、５０℃で２４時間乾燥し、その後、細かく微粉砕し、本発明品９９０ｇを得た。
（実施例２）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に水１５００ｍｌを添加、塩酸でｐＨを落とし１０日間放置した。その後、絞り機で絞り、得た清澄液を中和して、本発明品１２００ｍｌと残渣７６０ｇを得た。
（実施例３）
実施例１で得られた本発明品５００ｇを用いて、実施例３と同様の操作を行い、別の本発明品１１９０ｍｌを得た。
【００１５】
（実施例４）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に液化酵素１０ｇと水１５００ｍｌを添加した。その後、徐々に温度を上げていき、５分間煮沸抽出した後、冷却した。その後、絞り機で絞り、本発明品１４２０ｍｌと残渣５６０ｇを得た。
（実施例５）
実施例１で得られた本発明品５００ｇを用いて、実施例４と同様の操作を行い、別の本発明品１４００ｍｌを得た。
（実施例６）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に２Ｎ−ＮａＯＨ１５００ｍｌを添加して５日間放置した。その後、絞り機で絞り、清澄液１３５０ｍｌと残渣６５０ｇを得た。この清澄液を１０Ｎ−ＨＣｌで中和して、本発明品１４８０ｍｌを得た。
【００１６】
（実施例７）
実施例１で得られた本発明品５００ｇを用いて、実施例６と同様の操作を行い、別の本発明品１４９０ｍｌを得た。
（実施例８）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に９５％エタノール１５００ｍｌを添加して、５日間放置した。その後、絞り機で絞り、清澄液１３００ｍｌと残渣６５０ｇを得た。この清澄液に水２０００ｍｌを添加し、ロータリーエバプレーターで濃縮し、本発明品１５００ｍｌを得た。
（実施例９）
実施例１で得られた本発明品５００ｇを用いて、実施例８と同様の操作を行い、別の本発明品１５００ｍｌを得た。
【００１７】
（実施例１０）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に麹３００ｇ、水１５００ｍｌを加え、５５℃で２０時間放置した。その後、絞り機で絞り、本発明品１２３０ｍｌと残渣１０００ｇを得た。
（実施例１１）
実施例１で得られた本発明品５００ｇを用いて、実施例１０と同様の操作を行い、別の本発明品１２１０ｍｌを得た。
（実施例１２）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に蛋白分解酵素２ｇと水１５００ｍｌを加え、５０℃で２０時間放置した。その後、絞り機で絞り、本発明品１３１０ｍｌと残渣６７０ｇを得た。
【００１８】
（実施例１３）
実施例１で得られた本発明品５００ｇを用いて、実施例１２と同様の操作を行い、別の本発明品１３８０ｍｌを得た。
（実施例１４）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に脂肪分解酵素２ｇと水１５００ｍｌを加え、５０℃で２０時間放置した。その後、絞り機で絞り、本発明品１２９０ｍｌと残渣６８０ｇを得た。
（実施例１５）
実施例１で得られた本発明品５００ｇを用いて、実施例１４と同様の操作を行い、別の本発明品１３６０ｍｌを得た。
【００１９】
（実施例１６）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に繊維分解酵素２ｇと水１５００ｍｌを加え、５０℃で２０時間放置した。その後、絞り機で絞り、本発明品１３３０ｍｌと残渣６５０ｇを得た。
（実施例１７）
実施例１で得られた本発明品５００ｇを用いて、実施例１６と同様の操作を行い、別の本発明品１３７０ｍｌを得た。
（実施例１８）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に澱粉分解酵素２ｇと水１５００ｍｌを加え、５５℃で２０時間放置した。その後、絞り機で絞り、本発明品１３８０ｍｌと残渣６００ｇを得た。
【００２０】
（実施例１９）
実施例１で得られた本発明品５００ｇを用いて、実施例１８と同様の操作を行い、別の本発明品１４００ｍｌを得た。
（実施例２０）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物にペクチン分解酵素２ｇと水１５００ｍｌを加え、５０℃で２０時間放置した。その後、絞り機で絞り、本発明品１３２０ｍｌと残渣６６０ｇを得た。
（実施例２１）
実施例１で得られた本発明品５００ｇを用いて、実施例２０と同様の操作を行い、別の本発明品１３００ｍｌを得た。
【００２１】
（実施例２２）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に蛋白分解酵素２ｇ、脂肪分解酵素２ｇ、繊維分解酵素２ｇ、澱粉分解酵素２ｇ、ペクチン分解酵素２ｇと水１５００ｍｌを加え、５０℃で２０時間放置した。その後、絞り機で絞り、本発明品１４２０ｍｌと残渣５６０ｇを得た。
（実施例２３）
実施例１で得られた本発明品５００ｇを用いて、実施例２２と同様の操作を行い、別の本発明品１４４０ｍｌを得た。
（実施例２４）
実施例２２と同様の操作をして、米の酵素分解物２０００ｇを得た。その後、徐々に温度を上げていき、５分間煮沸抽出した後、冷却した。その後、絞り機で絞り、本発明品１４００ｍｌと残渣５５０ｇを得た。
【００２２】
（実施例２５）
実施例１で得られた本発明品５００ｇを用いて、実施例２４と同様の操作を行い、別の本発明品１４２０ｍｌを得た。
（実施例２６）
玄米を粉砕機にかけ、玄米の粉砕物５００ｇを得た。この粉砕物に麹３００ｇと４０％エタノール１５００ｍｌを加え、５５℃で４８時間放置した。その後、絞り機で絞り、清澄液１３００ｍｌと残渣８５０ｇを得た。その後、清澄液に１０００ｍｌの水を加水し、ロータリーエバプレーターで濃縮し、本発明品１３００ｍｌを得た。
（実施例２７）
実施例１で得られた本発明品５００ｇを用いて、実施例２６と同様の操作を行い、別の本発明品１３００ｍｌを得た。
【００２３】
（実施例２８）
実施例４と同様にして、米の抽出物２０００ｇを得た。この抽出物に蛋白分解酵素２ｇ、脂肪分解酵素２ｇ、繊維分解酵素２ｇ、澱粉分解酵素２ｇ、ペクチン分解酵素２ｇを添加し、５０℃で２４時間放置した。その後、絞り機で絞り、本発明品１４００ｍｌと残渣５８０ｇを得た。
（実施例２９）
実施例１で得られた本発明品５００ｇを用いて、実施例２８と同様の操作を行い、別の本発明品１３９０ｍｌを得た。
（実施例３０）
実施例２２と同様にして、米の酵素分解抽出物２０００ｇを得た。この酵素分解抽出物に酵母を添加し、１６日間アルコール発酵した。その後、絞り機で絞り、本発明品１８８０ｍｌと残渣８０ｇを得た。
【００２４】
（実施例３１）
実施例１で得られた本発明品５００ｇを用いて、実施例３０と同様の操作を行い、別の本発明品１８００ｍｌを得た。
（実施例３２）
実施例２２と同様にして、米の酵素分解抽出物２０００ｇを得た。この酵素分解抽出物を煮沸殺菌した後、３７℃まで冷却し、前もって乳酸菌を培養したスターター２００ｍｌを添加後、よく攪拌密封し、３７℃で２日間乳酸発酵を行った。その後、絞り機で絞り、本発明品１３８０ｍｌと残渣５９０ｇを得た。
（実施例３３）
実施例１で得られた本発明品５００ｇを用いて、実施例３２と同様の操作を行い、別の本発明品１４００ｍｌを得た。
【００２５】
（実施例３４）
実施例２２で得られた本発明品１０００ｍｌに９５％エタノール８０ｍｌを添加し、２０日間酢酸発酵を行った。その後、濾過をし、本発明品９９０ｍｌを得た。
（実施例３５）
実施例１で得られた本発明品５００ｇを用いて、実施例３４と同様の操作を行い、別の本発明品１０００ｍｌを得た。
本発明品を配合して錠剤とする場合、および清涼飲料とする場合の実施例について、次に記載する。なお、配合例は以下の実施例に限定されるものではない。
【００２６】
（実施例３６）錠剤
実施例２４で得られた本発明品１００ｇをフリーズドライにより乾燥し、２０ｇの乾燥品を得た。この乾燥品１０ｇを下記のようにして、錠剤を得た。
本発明品１０ｇ
ポリエチレングリコール６０００１０ｇ
ラウリル硫酸ナトリウム１．５ｇ
コーンスターチ３ｇ
乳糖２５ｇ
ステアリン酸マグネシウム０．５ｇ
上記成分を秤量した後、ポリエチレングリコール６０００を７０〜８０℃に加温し、これに本発明品、ラウリル硫酸ナトリウム、コーンスターチおよび乳糖を加え混合後、そのまま冷却する。固化した混合物を粉砕器にかけ造粒する。本顆粒をステアリン酸マグネシウムと混合後圧縮打錠して、重量２５０ｍｇの錠剤とする。
【００２７】
（実施例３７）清涼飲料
実施例２２で得られた本発明品１５重量％
甘草エキス０．０１重量％
砂糖４重量％
レモン果汁２．５重量％
精製水７８．４９重量％
常法により混合攪拌し、清涼飲料水を得た。
【００２８】
【発明の効果】
本発明品は、継続的使用により、高脂血症の発症予防に顕著な効果を示す。このように顕著な効果を示すものが、安全性が実証されている米から得られたことは画期的なことである。高脂血症によって起る虚血性心疾患の有病率は、４５才以上では、近年コンスタントに増え続けている。したがって、成人病に悩む人に大きな福音をもたらすものである。また、米の自由化の問題でゆれる日本の農業にとって、その消費拡大は死活問題である。その点においても大きな朗報であり、その波及効果は大きい。[0001]
[Industrial application fields]
The present invention relates to an antihyperlipidemic agent that can be used in the fields of pharmaceuticals, foods and the like obtained from rice or germinated rice as a raw material.
[0002]
[Prior art]
Japanese causes of death are cancer, heart disease, and cerebrovascular disease in that order, but the sum of heart disease and cerebrovascular disease is overwhelmingly higher than cancer. Therefore, prevention of arteriosclerosis is the most important issue for a healthy life.
As factors causing arteriosclerosis, particularly angina pectoris and myocardial infarction, three main factors are hypertension, smoking, and hyperlipidemia. Although antihypertensive drugs with few side effects have appeared in hypertension and their control has become easier, the management of dietary life is the most important for hyperlipidemia at present.
For example, the relationship between dietary fats (especially cholesterol) and ischemic heart disease has already been clarified. In the United States, the dietary habits have been reviewed about 20 years ago. A campaign to lower the price was developed.
[0003]
[Problems to be solved by the invention]
Any food that has been demonstrated to be safe and that suppresses the excessive accumulation of lipid components in the blood (ie, hyperlipidemia) can prevent arteriosclerosis and is expected to be developed.
An object of the present invention is to provide an antihyperlipidemic agent that is safe and inexpensive, has a stable supply of raw materials, is easy to process, and is completely safe even after regular use over a long period of time. It is.
[0004]
[Means for Solving the Problems]
The inventors of the present invention have been researching various plant components, mainly rice, which is a staple food, from the viewpoint of combining plants and animals. In the process, it has been found that rice has many possibilities and benefits that could not have been predicted before. Therefore, we have taken up the theme of rice, which is used as a staple food and has proven to be the safest, and has conducted comprehensive rice research. As one of the themes, we have conducted extensive research on antihyperlipidemic agents from rice. In the process, rice and germinated rice have an effect as antihyperlipidemic agents. The present inventors have found that the components are contained and have completed the present invention.
That is, the present invention (1) is an antihyperlipidemic agent containing pulverized white rice as an active ingredient.
The present invention (2) is an antihyperlipidemic agent containing a white rice extract as an active ingredient.
The present invention (3) is an antihyperlipidemic agent containing as an active ingredient a product obtained by enzymatically degrading or hydrolyzing white rice hydrolyzate.
The present invention (4) is an antihyperlipidemic agent containing, as an active ingredient, an enzyme-decomposed or koji-acted substance before, simultaneously with or after extraction of white rice.
The present invention (5) is an antihyperlipidemic agent containing, as an active ingredient, a product obtained by subjecting an extract of white rice or enzymatic degradation or koji to alcohol fermentation or organic acid fermentation.
The present invention (6) is the antihyperlipidemic agent according to any one of the inventions (1) to (5), wherein white rice is used in the form of white rice, brown rice or germinated rice.
The present invention (7) is the antihyperlipidemic agent of any one of the inventions (1) to (6) for anti-arteriosclerosis.
[0005]
In the present invention, ingredients having an effect as an antihyperlipidemic agent contained in rice and germinated rice have not yet been elucidated, but the rice and germinated rice are treated as follows. It has been found that the resulting product exhibits an effect as an antihyperlipidemic agent.
(1) A rice pulverized rice as it is or containing it.
(2) Rice or germinated rice extract as it is or containing it.
(3) Rice or germinated rice hydrolyzate that has been subjected to enzymatic degradation or koji is used as it is or contains it.
{Circle around (4)} Extracting rice or germinated rice as it is, or containing it, that has been subjected to enzymatic degradation or koji before, simultaneously with or after extraction.
(5) Rice or germinated rice extract or enzyme-decomposed or rice bran that is subjected to alcoholic fermentation or organic acid fermentation as it is or contains it.
[0006]
The rice used in the present invention refers to brown rice and white rice such as glutinous rice and glutinous rice, regardless of japonica and indica rice, regardless of the variety and type. Furthermore, it is possible to use 92% or more of red cocoon that appears during whitening, or 92% or less of white cocoon, which is inexpensive and economical. In addition, germinated rice is used. In addition, since the active ingredient is stable to heat and light, the above-mentioned raw materials are dipping, steaming, roasting (pointing to all of sand roasting, net roasting, hot air roasting, etc.), steaming roasting, freeze drying Material treatment such as surface modification such as UV irradiation, photo modification such as UV irradiation, pressure roasting such as Patrice, frying, etc., and the effect was not changed.
Rice and germinated rice are effective when used as they are, but are preferably pulverized for practical use. In order to pulverize rice and germinated rice into powder, a general method may be used using a pulverizer or a rice mill.
[0007]
When germinating rice, the germinated rice is immersed in water or sprayed with water. The temperature at the time of germination is 5-70 degreeC. However, the temperature and time are not limited as long as germination occurs. In addition, when there is a risk of water rot during germination, it is preferable to replace the water so that it does not rot or to perform some preservative. Here, germination refers to everything from just before germination to germination. The germinated rice is washed thoroughly before use. At this time, you may dry and use.
When rice or germinated rice is extracted or subjected to enzymatic degradation or koji, if the raw rice is pulverized into granules or powders, the surface area increases and efficiency increases. Although it is not necessary to grind, in this case, it takes a long time to decompose and extract the rice tissue.
[0008]
When rice or germinated rice is extracted with water, a high extraction temperature is efficient, but sufficient extraction can be performed even at a low temperature. However, in the case of a low temperature of 40 ° C. or lower, it is desirable that the pH is made acidic or alkaline, or a preservative or alcohol is added to prevent the rice from being spoiled. The extraction time may be long or short as long as the active ingredient can be extracted, and may be determined by the extraction temperature and the extraction time. The extraction may be performed under pressure, normal pressure, or reduced pressure.
In the case of water extraction, the most serious problem is the gelatinization phenomenon. If it becomes paste-like, not only extraction efficiency will worsen but it will be extremely difficult in actual work. In order to prevent this, the reaction may be performed by adding amylase or acidifying with hydrochloric acid or the like to cut the starch. By using this method, the problem can be solved sufficiently and there is no problem in practical use.
[0009]
It is also effective to perform acid decomposition extraction or alkali decomposition extraction because the active ingredient in the extract is stable to acid and alkali. In this case, neutralization and desalting are performed as necessary.
Also when extracting with an organic solvent, it is desirable to extract rice by pulverizing or pulverizing it as much as possible. The organic solvent may be a common organic solvent such as alcohol, acetone, n-hexane, methanol or the like, but those which are harmful to the human body are preferably safe because the solvent needs to be completely removed after extraction.
In addition, rice or germinated rice may be subjected to enzymatic degradation, or koji. The term “enzymatic degradation” as used herein means that one or more enzymes acting on rice such as starch degrading enzyme, proteolytic enzyme, lipolytic enzyme, fiber degrading enzyme, lignin degrading enzyme, and pectin degrading enzyme are allowed to act. In addition, the type of koji mold and the variety and type of rice are not questioned.
[0010]
Furthermore, in performing the said extraction, you may make said enzyme decomposition | disassembly and soot act before extraction, simultaneous with extraction, or after extraction.
In the present invention, when organic acid fermentation such as alcohol fermentation, lactic acid fermentation, or acetic acid fermentation is performed simultaneously with or after the above treatment, the following points are effective.
First, if alcoholic fermentation is performed, concentration is easy and concentration of active ingredients becomes easy. In addition, when lactic acid fermentation is used for beverages, etc., the flavor is improved, and acetic acid fermentation can be used as a seasoning liquid application of vinegar, and can be used as a wide range of applications by organic acid fermentation. Can do.
In addition, when the red cocoon portion of 92% or more was examined, it was found that although there was an effect, it was weak.
[0011]
The product of the present invention obtained as described above is used as it is, or after being squeezed and filtered without separating the residue. Moreover, when using as it is, it is used after sterilizing or sterilizing. You may dry and use it as a powder, a granule, a tablet, etc. Furthermore, it can also mix | blend and use for various foods.
Hereinafter, the results of specifically examining the antihyperlipidemic effect of the product of the present invention will be described.
Four-week-old male ddY mice were raised for one week in an animal room maintained at room temperature of 25 ° C. and humidity of 60%, and were allowed to freely ingest water, and then subjected to experiments. The experiment was conducted with 10 animals per group. The test liquid was placed in a water bottle at 20 am once a day at 10 am and allowed to ingest freely. Four weeks after administration, whole blood was collected from the carotid artery under ether anesthesia, and after processing necessary for quantitative operation, blood components were analyzed.
The results are shown in Table 1.
[0012]
[Table 1]

[0013]
As shown in Table 1, the blood VLDL concentration was significantly decreased in the group administered with the present invention. The HDL-cholesterol level increased in the product-administered group. HDL-cholesterol has a function of transferring cholesterol from peripheral tissues to the liver, and is responsible for removing intracellular cholesterol. The blood triglyceride and total lipid showed a decrease in the group administered with the present invention.
The above results indicate that the product of the present invention clearly has an action of reducing lipid components in blood. Therefore, it was found that the product of the present invention is extremely effective for preventing the onset of hyperlipidemia.
[0014]
【Example】
Example 1
1 kg of rice with germs was placed in water at 25 ° C. and immersed for 3 days to germinate the rice. After thoroughly washing the germinated rice, it was dried at 50 ° C. for 24 hours, and then finely pulverized to obtain 990 g of the product of the present invention.
(Example 2)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 1500 ml of water was added, and the pH was lowered with hydrochloric acid and left for 10 days. Thereafter, the clarified liquid obtained by squeezing with a squeezer was neutralized to obtain 1200 ml of the present product and 760 g of a residue.
Example 3
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 3 was performed to obtain 1190 ml of another product of the present invention.
[0015]
(Example 4)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. 10 g of liquefied enzyme and 1500 ml of water were added to this pulverized product. Thereafter, the temperature was gradually increased, followed by boiling extraction for 5 minutes and then cooling. Thereafter, the product was squeezed with a squeezer to obtain 1420 ml of the product of the present invention and 560 g of residue.
(Example 5)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 4 was performed to obtain 1400 ml of another product of the present invention.
(Example 6)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 1500 ml of 2N-NaOH was added and left for 5 days. Then, it squeezed with the squeezer and obtained 1350 ml of clarified liquids, and 650 g of residue. The clear solution was neutralized with 10N HCl to obtain 1480 ml of the product of the present invention.
[0016]
(Example 7)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 6 was performed to obtain another 1490 ml of the product of the present invention.
(Example 8)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 1500 ml of 95% ethanol was added and left for 5 days. Then, it squeezed with the squeezer and 1300 ml of clarified liquids and 650 g of residue were obtained. To this clarified liquid, 2000 ml of water was added and concentrated with a rotary evaporator to obtain 1500 ml of the product of the present invention.
Example 9
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 8 was performed to obtain 1500 ml of another product of the present invention.
[0017]
(Example 10)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 300 g of candy and 1500 ml of water were added, and the mixture was left at 55 ° C. for 20 hours. Then, it squeezed with the squeezer and obtained 1230 ml of this invention products and 1000 g of residue.
(Example 11)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 10 was performed to obtain 1210 ml of another product of the present invention.
(Example 12)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 2 g of proteolytic enzyme and 1500 ml of water were added and left at 50 ° C. for 20 hours. Then, it squeezed with the squeezer and obtained 1310 ml of this invention products and 670 g of residue.
[0018]
(Example 13)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 12 was performed to obtain 1380 ml of another product of the present invention.
(Example 14)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 2 g of lipolytic enzyme and 1500 ml of water were added and left at 50 ° C. for 20 hours. Thereafter, the product was squeezed with a squeezer to obtain 1290 ml of the product of the present invention and 680 g of residue.
(Example 15)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 14 was performed to obtain 1360 ml of another product of the present invention.
[0019]
(Example 16)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 2 g of a fiber-degrading enzyme and 1500 ml of water were added and left at 50 ° C. for 20 hours. Thereafter, the product was squeezed with a squeezer to obtain 1330 ml of the present product and 650 g of a residue.
(Example 17)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 16 was performed to obtain 1370 ml of another product of the present invention.
(Example 18)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 2 g of amylolytic enzyme and 1500 ml of water were added and left at 55 ° C. for 20 hours. Thereafter, the product was squeezed with a squeezer to obtain 1380 ml of the product of the present invention and 600 g of residue.
[0020]
(Example 19)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 18 was performed to obtain 1400 ml of another product of the present invention.
(Example 20)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 2 g of pectin-degrading enzyme and 1500 ml of water were added and left at 50 ° C. for 20 hours. Thereafter, the product was squeezed with a squeezer to obtain 1320 ml of the product of the present invention and 660 g of residue.
(Example 21)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 20 was performed to obtain 1300 ml of another product of the present invention.
[0021]
(Example 22)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme and 1500 ml of water were added and left at 50 ° C. for 20 hours. Thereafter, the product was squeezed with a squeezer to obtain 1420 ml of the product of the present invention and 560 g of residue.
(Example 23)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 22 was performed to obtain 1440 ml of another product of the present invention.
(Example 24)
The same operation as in Example 22 was performed to obtain 2000 g of an enzymatic degradation product of rice. Thereafter, the temperature was gradually increased, followed by boiling extraction for 5 minutes and then cooling. Then, it squeezed with the squeezer and obtained 1400 ml of this invention products and 550 g of residue.
[0022]
(Example 25)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 24 was performed to obtain 1420 ml of another product of the present invention.
(Example 26)
Brown rice was put into a pulverizer to obtain 500 g of pulverized brown rice. To this pulverized product, 300 g of koji and 1500 ml of 40% ethanol were added and left at 55 ° C. for 48 hours. Then, it squeezed with the squeezer and 1300 ml of clarified liquids and 850 g of residue were obtained. Thereafter, 1000 ml of water was added to the clarified liquid and concentrated with a rotary evaporator to obtain 1300 ml of the product of the present invention.
(Example 27)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 26 was performed to obtain 1300 ml of another product of the present invention.
[0023]
(Example 28)
In the same manner as in Example 4, 2000 g of rice extract was obtained. To this extract, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme and 2 g of pectin degrading enzyme were added and left at 50 ° C. for 24 hours. Thereafter, the product was squeezed with a squeezer to obtain 1400 ml of the present product and 580 g of a residue.
(Example 29)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 28 was performed to obtain another 1390 ml of the product of the present invention.
(Example 30)
In the same manner as in Example 22, 2000 g of enzymatic decomposition extract of rice was obtained. Yeast was added to this enzymatic degradation extract, and alcohol fermentation was performed for 16 days. Thereafter, the product was squeezed with a squeezer to obtain 1880 ml of the product of the present invention and 80 g of residue.
[0024]
(Example 31)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 30 was performed to obtain 1800 ml of another product of the present invention.
(Example 32)
In the same manner as in Example 22, 2000 g of enzymatic decomposition extract of rice was obtained. The enzyme-degraded extract was sterilized by boiling, cooled to 37 ° C., added with 200 ml of a starter in which lactic acid bacteria had been cultured in advance, sealed well, and subjected to lactic acid fermentation at 37 ° C. for 2 days. Thereafter, the product was squeezed with a squeezer to obtain 1380 ml of the present product and 590 g of a residue.
(Example 33)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 32 was performed to obtain 1400 ml of another product of the present invention.
[0025]
(Example 34)
To 1000 ml of the product of the present invention obtained in Example 22, 80 ml of 95% ethanol was added, and acetic acid fermentation was performed for 20 days. Thereafter, filtration was performed to obtain 990 ml of the present product.
(Example 35)
Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 34 was performed to obtain 1000 ml of another product of the present invention.
Examples of the case where the product of the present invention is blended to form tablets and the case of soft drinks are described below. In addition, a compounding example is not limited to a following example.
[0026]
(Example 36) 100 g of the product of the present invention obtained in Tablet Example 24 was dried by freeze drying to obtain 20 g of a dried product. 10 g of this dried product was obtained as follows to obtain a tablet.
Invention product 10g
Polyethylene glycol 6000 10g
Sodium lauryl sulfate 1.5g
Corn starch 3g
Lactose 25g
Magnesium stearate 0.5g
After weighing the above components, polyethylene glycol 6000 is heated to 70 to 80 ° C., the product of the present invention, sodium lauryl sulfate, corn starch and lactose are added and mixed, and then cooled as it is. The solidified mixture is granulated in a grinder. The granules are mixed with magnesium stearate and compressed into tablets with a weight of 250 mg.
[0027]
(Example 37) 15% by weight of the present invention product obtained in Soft Drink Example 22
Licorice extract 0.01% by weight
4% sugar
Lemon juice 2.5% by weight
Purified water 78.49% by weight
Mixing and stirring were conducted by a conventional method to obtain a soft drink.
[0028]
【The invention's effect】
The product of the present invention has a remarkable effect in preventing the onset of hyperlipidemia by continuous use. It is an epoch-making thing to obtain from such a rice which has demonstrated the remarkable effect that the safety has been proved. The prevalence of ischemic heart disease caused by hyperlipidemia has been constantly increasing at the age of 45 years and older. Therefore, it brings a great gospel to those who suffer from adult diseases. In addition, for Japanese agriculture, which is suffering from the issue of rice liberalization, the expansion of consumption is a matter of life and death. This is also great news, and the ripple effect is great.

Claims

Hypolipidemic agents containing white rice hydrolyzate as an active ingredient that is enzymatic degradation.

An antihyperlipidemic agent containing, as an active ingredient, an enzyme-decomposed product before or after the extraction of white rice.

Furthermore, the antihyperlipidemic agent according to claim 1 or 2, which has been subjected to alcoholic fermentation or organic acid fermentation .

The antihyperlipidemic agent according to any one of claims 1 to 3 , for anti-arteriosclerosis.