JPH07138176A - Anti-hyperlipemic agent from rice - Google Patents

Abstract

PURPOSE:To obtain a safe, inexpensive and readily processable anti-hyperlipemic agent, stably supplied with raw materials and entirely safe even by common use thereof for a long period. CONSTITUTION:This anti-hyperlipemic agent from rice is intactly each of the following substances or a material containing each thereof: (1) a pulverized substance of germinated rice, (2) an extract from rice or the germinated rice, (3) a substance prepared by the action of enzymolysis or KOJI (yeast) on the rice or germinated rice, (4) a substance obtained by the action of the enzymolysis or KOJI on the rice or germinated rice before, simultaneously with or after extraction thereof in extracting the rice or germinated rice and (5) a substance prepared by carrying out the alcohol fermentation or organic acid fermentation of the extract from the rice or germinated rice or the substance obtained by the action of the enzymolysis or KOJI on the extract.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antihyperlipidemic agent which can be obtained from rice or sprouted rice as a raw material and can be used in the fields of medicine, food and the like.

[0002]

2. Description of the Related Art Causes of death in Japanese are cancer, heart disease and cerebrovascular disease in this order, but the total of heart disease and cerebrovascular disease is overwhelmingly greater than that of cancer. Therefore, prevention of arteriosclerosis has become the most important issue for a healthy life. Hypertension, smoking, and hyperlipidemia are considered to be the three major factors that cause arteriosclerosis, particularly angina and myocardial infarction. With regard to hypertension, antihypertensive drugs with few side effects have appeared and it has become easy to control them, but for hyperlipidemia, it is the current situation that dietary management is of utmost importance. For example, the relationship between dietary fat (particularly cholesterol) and ischemic heart disease has already been clarified. In the United States, about 20 years ago, we began to review our dietary habits, and improved it to improve blood cholesterol levels. The campaign to lower the price was developed.

[0003]

[Problems to be Solved by the Invention] If there is a food that has been proved to be safe and that suppresses excessive accumulation of lipid components in blood (ie, hyperlipidemia), it will prevent arteriosclerosis, Its development is awaited. An object of the present invention is to provide an antihyperlipidemic agent which is safe and inexpensive, has a stable supply of raw materials, is easy to process, and is completely safe even when used for a long period of time. Is.

[0004]

[Means for Solving the Problems] From the viewpoint of animal and plant harmony, the present inventors have conducted research on various plant components centering on rice, which is a staple food. In the process, it has become clear that rice has a number of potential and benefits that were previously unpredictable. Therefore, we picked up rice, which is used as a staple food and proved to have the highest safety, as the theme,
I have conducted comprehensive utilization research on rice. As one of the themes, we have conducted intensive research on antihyperlipidemic agents from rice, and in the process, rice and germinated rice have an effect as antihyperlipidemic agents. It was found that the components were contained, and the present invention was completed.

In the present invention, the components contained in rice and germinated rice and having an effect as an antihyperlipidemic agent have not yet been elucidated, but rice and germinated rice are described below. Those treated in this way were found to exhibit effects as antihyperlipidemic agents. Sprouted crushed rice as it is or containing it. Rice or germinated rice extract as it is or containing it. A product obtained by enzymatically decomposing or hydrolyzing malted rice and hydrolyzed rice as it is, or containing it. When extracting rice or sprouted rice, the one that has been subjected to enzymatic decomposition or koji before or at the same time as or after the extraction is used as it is or containing it. An extract of rice or germinated rice or a product of enzymatic decomposition or koji which has been subjected to alcohol fermentation or organic acid fermentation as it is or containing it.

Rice used in the present invention means japonica,
Regardless of indica rice, it refers to non-glutinous rice, brown rice such as sticky rice, and white rice, regardless of variety and type. Further, 92% or more of red rice bran or 92% or less of white rice bran, which appears during whitening, may be used, which is inexpensive and economical. Also,
Germinated rice is used. In addition, since the active ingredient is stable to heat and light, the above raw materials are dipping, steaming, roasting (all sand roasting, net roasting, hot air roasting, etc.),
The raw material treatment such as steam roasting, surface modification such as freeze-drying, photo-modification such as UV irradiation, pressure roasting such as Patrice, and frying may be performed, and the effect was not changed. Although rice and germinated rice are effective as they are, they are preferably crushed and used from the viewpoint of practical use. In order to pulverize the rice and the sprouted rice into powder, a pulverizer or a rice mill may be used by a general method.

When germinating rice, germinated rice is soaked in water or sprayed with water to germinate. The temperature for germination is 5 to 70 ° C. However, as long as it germinates,
Temperature and time do not matter. Further, when there is a risk of water spoiling during germination, it is preferable to replace the water so that it does not spoil, or to perform some kind of preservative. Here, germination refers to everything from just before germination to germinated ones.
The germinated rice is washed well before use. At this time, it may be dried before use. When extracting rice or sprouted rice, or subjecting it to enzymatic decomposition or koji, the raw material rice is pulverized into granules or powder to increase the surface area, resulting in higher efficiency. Although it is not necessary to grind, in this case, it takes a long time to decompose and extract the rice tissue.

When water or sprouted rice is extracted with water,
High extraction temperature is efficient, but extraction can be sufficiently performed even at low temperature. However, at a low temperature of 40 ° C. or lower, it is desirable to make the pH acidic or alkaline, or add a preservative or alcohol to treat the rice so that it does not spoil. The extraction time may be long or short as long as the active ingredient can be extracted, and may be determined depending on the extraction temperature and the extraction time. The extraction may be carried out under pressure, at normal pressure, or under reduced pressure. For water extraction,
The most problem is the gelatinization phenomenon. If it becomes pasty, not only the extraction efficiency will deteriorate, but it will be extremely difficult in actual work. In order to prevent this, it suffices to add amylase to react, or acidify with starch such as hydrochloric acid and cut the starch. By using this method, it can be sufficiently solved,
There is no problem in practice.

Since the active ingredient in the extract is stable to acid and alkali, it is also effective to perform acid decomposition extraction or alkali decomposition extraction. In this case, neutralize if necessary,
Desalt. Also when extracting with an organic solvent, it is desirable to pulverize or pulverize rice as much as possible before extracting. The organic solvent may be a general organic solvent such as alcohol, acetone, n-hexane, methanol, etc., but if it is harmful to the human body, it is necessary to completely remove the solvent after extraction, so a safe one is preferable. In addition, rice or germinated rice may be enzymatically decomposed or koji may be allowed to act. Enzymatic degradation here means starch degrading enzyme, proteolytic enzyme, lipolytic enzyme,
It refers to the action of one or more enzymes that act on rice, such as fiber degrading enzymes, lignin degrading enzymes, and pectin degrading enzymes. Moreover, the type of koji mold and the variety and type of rice as koji do not matter.

Further, in carrying out the above-mentioned extraction, the above-mentioned enzymatic decomposition and koji may be applied before the extraction, at the same time as the extraction or after the extraction. In the present invention, at the same time as or after the above treatment, alcohol fermentation or lactic acid fermentation, and organic acid fermentation such as acetic acid fermentation,
It is effective in the following points. First, if alcohol fermentation is carried out, it is easy to concentrate, and the active ingredient is also easily concentrated. Further, when lactic acid fermentation is used for applications such as beverages, the product of the present invention can be used as a seasoning liquid application of acetic acid fermentation to improve flavor, and acetic acid fermentation can be used as a wide range of applications by organic acid fermentation. You can Also, 92%
When the above red bran part was examined, it was found to be effective but weak.

The product of the present invention obtained as described above is used as it is without separating the residue, or after being pressed and filtered. When used as it is, it is sterilized or sterilized before use. You may use it after making it a powder, a granule, a tablet, etc. after drying. Furthermore, it can be used by being mixed with various foods. Hereinafter, the results of specifically examining the antihyperlipidemic effect of the product of the present invention will be described. Four-week-old male ddY mice were placed in an animal room kept at room temperature of 25 ° C and humidity of 60%.
After feeding for a week and water ad libitum, the animals were subjected to experiments. The experiment was performed with 10 animals per group. 20 ml per group of the test solution was placed once a day at 10 am in a water bottle and freely ingested. Four weeks after the administration, whole blood was collected from the carotid artery under ether anesthesia, and after processing necessary for quantitative operation, blood components were analyzed. The results are shown in Table 1.

[0012]

[Table 1]

As shown in Table 1, the blood VLDL concentrations were significantly decreased in the groups administered with the product of the present invention. HD
The L-cholesterol level was increased in the group administered with the product of the present invention. HDL-cholesterol has a function of transferring cholesterol from peripheral tissues to the liver and is responsible for removing intracellular cholesterol. Blood triglycerides and total lipids showed a decrease in the group administered with the present invention. The above results clearly show that the product of the present invention has an action of reducing lipid components in blood. Therefore, it was revealed that the product of the present invention is extremely effective in preventing the development of hyperlipidemia.

[0014]

【Example】

(Example 1) 1 kg of rice without germ was soaked in water at 25 ° C for 3 days to germinate rice. After thoroughly washing the germinated rice, it is dried at 50 ° C. for 24 hours, and then,
The product was finely pulverized to obtain 990 g of the product of the present invention. (Example 2) Brown rice was crushed to obtain a crushed brown rice product 500
g was obtained. 1500 ml of water was added to this pulverized product, the pH was lowered with hydrochloric acid, and the mixture was left for 10 days. Then, it was squeezed with a squeezing machine to neutralize the resulting clear liquid to obtain 1200 ml of the product of the present invention and 760 g of a residue. (Example 3) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 3 was carried out to obtain another product of the present invention 11
90 ml was obtained.

(Example 4) Brown rice was crushed to obtain 500 g of crushed brown rice. Liquefaction enzyme 10g and water 1500ml were added to this ground product. Then, the temperature was gradually raised, and the mixture was boiled and extracted for 5 minutes and then cooled. Then, it was squeezed with a squeezing machine to obtain 1420 ml of the product of the present invention and 560 g of a residue. (Example 5) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 4 was carried out to obtain another product of the present invention 14
00 ml was obtained. (Example 6) Brown rice is crushed by a crusher to obtain crushed brown rice 500
g was obtained. 1500 ml of 2N-NaOH was added to this pulverized product and the mixture was left for 5 days. Then, it was squeezed with a squeezing machine to obtain 1350 ml of the clear liquid and 650 g of the residue. 1 of this clarified liquid
Neutralization with 0N-HCl gave 1480 ml of the product of the present invention.

Example 7 Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 6 was carried out to obtain another 1490 ml of the product of the present invention. (Embodiment 8) Brown rice is crushed to obtain a crushed brown rice product 500
g was obtained. 1500 ml of 95% ethanol is added to this pulverized product.
Was added and left for 5 days. After that, squeeze with a wringer,
1300 ml of clear liquid and 650 g of residue were obtained. 2000 ml of water was added to this clarified liquid and concentrated by a rotary evaporator to obtain 1500 ml of the product of the present invention. (Example 9) Using 500 g of the present invention product obtained in Example 1, the same operation as in Example 8 was carried out to obtain another invention product 15
00 ml was obtained.

Example 10 Brown rice was crushed to obtain 500 g of crushed brown rice. 300 g of koji and 1500 ml of water were added to this pulverized product, and the mixture was left at 55 ° C. for 20 hours. Then, squeezing with a squeezing machine, 1230 ml of the present invention product and 100 residues
0 g was obtained. (Example 11) Using 500 g of the present invention product obtained in Example 1, the same operation as in Example 10 was carried out to obtain another 1210 ml of the present invention product. (Example 12) Brown rice is crushed to obtain a crushed brown rice product 50
0 g was obtained. 2 g of protease and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, the product was squeezed with a squeezing machine to obtain 1310 ml of the product of the present invention and 670 g of a residue.

(Example 13) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 12 was carried out,
Another 1380 ml of the product of the present invention was obtained. (Example 14) Brown rice is crushed to obtain 50 crushed brown rice.
0 g was obtained. 2 g of lipolytic enzyme and 150 water
0 ml was added and the mixture was left at 50 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1290 ml of the product of the present invention and 680 g of a residue. (Example 15) The same operation as in Example 14 was carried out using 500 g of the product of the present invention obtained in Example 1 to obtain 1360 ml of another product of the present invention.

Example 16 Brown rice was crushed to obtain 500 g of crushed brown rice. Fiber-degrading enzyme 2 in this crushed product
g and 1500 ml of water were added, and the mixture was left at 50 ° C. for 20 hours. Then, the product was squeezed with a squeezing machine to obtain 1330 ml of the product of the present invention and 650 g of a residue. (Example 17) The same operation as in Example 16 was carried out using 500 g of the product of the present invention obtained in Example 1 to obtain 1370 ml of another product of the present invention. (Example 18) Brown rice was crushed to obtain a crushed brown rice product 50
0 g was obtained. 2g starch degrading enzyme and 150g water
0 ml was added and the mixture was left at 55 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1380 ml of the product of the present invention and 600 g of a residue.

(Example 19) The same operation as in Example 18 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1400 ml of the product of the present invention was obtained. (Example 20) Brown rice is crushed to obtain a crushed brown rice product 50
0 g was obtained. Add 2 g of pectin-degrading enzyme and 1 part of water to this ground product.
500 ml was added and left at 50 ° C. for 20 hours. After that, squeezing with a squeezing machine, 1320 ml of the present invention product and residue 660
g was obtained. (Example 21) Using 500 g of the present invention product obtained in Example 1, the same operation as in Example 20 was carried out to obtain another 1300 ml of the present invention product.

Example 22 Brown rice was crushed to obtain 500 g of crushed brown rice. Proteolytic enzyme 2 in this crushed product
g, lipolytic enzyme 2 g, fiber degrading enzyme 2 g, starch degrading enzyme 2 g, pectin degrading enzyme 2 g and 1500 ml of water were added, and the mixture was allowed to stand at 50 ° C. for 20 hours. Then, it was squeezed with a squeezing machine to obtain 1420 ml of the product of the present invention and 560 g of a residue. (Example 23) Using 500 g of the product of the present invention obtained in Example 1, the same operation as in Example 22 was carried out to obtain another 1440 ml of the product of the present invention. (Example 24) The same operation as in Example 22 was carried out to obtain 2000 g of an enzymatic decomposition product of rice. Then, the temperature was gradually raised, and the mixture was boiled and extracted for 5 minutes and then cooled. Then, it was squeezed with a squeezing machine to obtain 1400 ml of the product of the present invention and 550 g of a residue.

(Example 25) The same operation as in Example 24 was carried out using 500 g of the product of the present invention obtained in Example 1,
1420 ml of another product of the present invention was obtained. (Example 26) Brown rice was crushed to obtain a crushed brown rice product 50
0 g was obtained. To this crushed product, 300 g of koji and 1500 ml of 40% ethanol were added, and the mixture was left at 55 ° C. for 48 hours. After that, squeeze with a squeezing machine and clarified liquid 1300 ml and residue 850
g was obtained. Then, 1000 ml of water was added to the clarified solution and concentrated with a rotary evaporator to obtain the product of the present invention 13
00 ml was obtained. (Example 27) The same operation as in Example 26 was carried out using 500 g of the present invention product obtained in Example 1 to obtain another 1300 ml of the present invention product.

(Example 28) In the same manner as in Example 4, 2000 g of a rice extract was obtained. To this extract, 2 g of proteolytic enzyme, 2 g of lipolytic enzyme, 2 g of fiber degrading enzyme, 2 g of starch degrading enzyme, 2 g of pectin degrading enzyme were added, and the mixture was heated at 50 ° C for 2
It was left for 4 hours. After that, the product of the present invention 14
00 ml and 580 g of residue were obtained. (Example 29) The same operation as in Example 28 was carried out using 500 g of the present invention product obtained in Example 1 to obtain another 1390 ml of the present invention product. (Example 30) In the same manner as in Example 22, 2000 g of an enzyme-decomposed extract of rice was obtained. Yeast was added to this enzyme-decomposed extract, and alcoholic fermentation was carried out for 16 days. Then, it was squeezed with a squeezing machine to obtain 1880 ml of the product of the present invention and 80 g of a residue.

(Example 31) The same operation as in Example 30 was carried out using 500 g of the product of the present invention obtained in Example 1,
Another 1800 ml of the product of the present invention was obtained. (Example 32) In the same manner as in Example 22, 2000 g of an enzyme-decomposed extract of rice was obtained. The enzyme-decomposed extract was sterilized by boiling, cooled to 37 ° C., 200 ml of a starter in which lactic acid bacteria had been cultured in advance was added, and the mixture was well stirred and sealed, and
Lactic acid fermentation was performed at 7 ° C for 2 days. Then, it was squeezed with a squeezing machine to obtain 1380 ml of the product of the present invention and 590 g of a residue. (Example 33) Using 500 g of the present invention product obtained in Example 1, the same operation as in Example 32 was carried out to obtain another 1400 ml of the present invention product.

(Example 34) To 1000 ml of the product of the present invention obtained in Example 22 was added 80 ml of 95% ethanol, and acetic acid fermentation was carried out for 20 days. Then, filtration was performed to obtain 990 ml of the product of the present invention. (Example 35) Using 500 g of the present invention product obtained in Example 1, the same operation as in Example 34 was carried out to obtain another 1000 ml of the present invention product. Examples of blending the product of the present invention into a tablet and a soft drink will be described below. The formulation examples are not limited to the examples below.

(Example 36) Tablets 100 g of the product of the present invention obtained in Example 24 was dried by freeze drying to obtain 20 g of a dried product. This dried product 1
Tablets were obtained from 0 g as described below. Product of the present invention 10 g Polyethylene glycol 6000 10 g Sodium lauryl sulfate 1.5 g Corn starch 3 g Lactose 25 g Magnesium stearate 0.5 g Polyethylene glycol 600 after weighing the above components
0 is heated to 70 to 80 ° C., the product of the present invention, sodium lauryl sulfate, corn starch and lactose are added thereto, mixed and then cooled as it is. The solidified mixture is crushed by a grinder. The granules are mixed with magnesium stearate and compressed into tablets to give tablets having a weight of 250 mg.

(Example 37) Soft drink The product of the present invention obtained in Example 22 15% by weight Licorice extract 0.01% by weight Sugar 4% by weight Lemon juice 2.5% by weight Purified water 78.49% by weight Conventional method Was mixed and stirred to obtain soft drink.

[0028]

INDUSTRIAL APPLICABILITY The product of the present invention exhibits a remarkable effect in preventing the onset of hyperlipidemia by continuous use. It is epoch-making that such a remarkable effect was obtained from rice whose safety has been proven. The prevalence of ischemic heart disease caused by hyperlipidemia has been constantly increasing in recent years after the age of 45. Therefore, it brings great gospel to those who suffer from adult diseases. Also, for Japanese agriculture, which is shaken by the issue of rice liberalization, expanding its consumption is a life and death problem. This is also great news, and its ripple effect is great.

Claims (5)

[Claims] 1. An antihyperlipidemic agent comprising a crushed product of germinated rice as it is or containing it. 2. An antihyperlipidemic agent comprising rice or an extract of germinated rice as it is or containing the extract. 3. An antihyperlipidemic agent, which is obtained by enzymatically decomposing or hydrolyzing rice hydrolyzed with hydrolyzed rice, or containing it. 4. When extracting rice or sprouted rice, enzyme-decomposed or koji-acted ones before or at the same time as or after extraction, as they are, or containing antihyperlipidemia. Drug. 5. An antihyperlipidemic product comprising the extract of rice or germinated rice or the product of enzymatic decomposition or koji which has been subjected to alcohol fermentation or organic acid fermentation as it is or containing it. Drug.