CN105613937A - Preparation method of momordica charantia p-polypeptide freeze-dried powder - Google Patents
Preparation method of momordica charantia p-polypeptide freeze-dried powder Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 239000000843 powder Substances 0.000 title claims abstract description 22
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- 239000002994 raw material Substances 0.000 claims abstract description 25
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- 235000019634 flavors Nutrition 0.000 claims abstract description 18
- 108091005804 Peptidases Proteins 0.000 claims abstract description 16
- 239000004365 Protease Substances 0.000 claims abstract description 16
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 16
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- 238000005119 centrifugation Methods 0.000 claims abstract description 6
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- 238000003756 stirring Methods 0.000 claims description 23
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- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 16
- 238000011282 treatment Methods 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 12
- 238000000227 grinding Methods 0.000 claims description 11
- 238000001556 precipitation Methods 0.000 claims description 11
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 6
- 238000001035 drying Methods 0.000 abstract description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 2
- 240000006432 Carica papaya Species 0.000 abstract 1
- 235000009467 Carica papaya Nutrition 0.000 abstract 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract 1
- 229930003268 Vitamin C Natural products 0.000 abstract 1
- 238000003916 acid precipitation Methods 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 238000004090 dissolution Methods 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 235000019659 mouth feeling Nutrition 0.000 abstract 1
- 239000008280 blood Substances 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 12
- 206010012601 diabetes mellitus Diseases 0.000 description 9
- 102000004877 Insulin Human genes 0.000 description 6
- 108090001061 Insulin Proteins 0.000 description 6
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
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- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 229940125396 insulin Drugs 0.000 description 3
- 235000009812 Momordica cochinchinensis Nutrition 0.000 description 2
- 235000018365 Momordica dioica Nutrition 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 229940127017 oral antidiabetic Drugs 0.000 description 2
- 239000003538 oral antidiabetic agent Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 208000013016 Hypoglycemia Diseases 0.000 description 1
- 102000003746 Insulin Receptor Human genes 0.000 description 1
- 108010001127 Insulin Receptor Proteins 0.000 description 1
- 208000022329 Protein metabolism disease Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
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- 239000004615 ingredient Substances 0.000 description 1
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- 230000002045 lasting effect Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Seasonings (AREA)
Abstract
The invention discloses a preparation method of momordica charantia p-polypeptide freeze-dried powder and belongs to the technical field of biological medicines. The method comprises the following steps: raw material pretreatment, degreasing, alkali dissolution, enzymolysis, acid precipitation, centrifugation, washing, freezing and drying. An enzyme used for the enzymolysis contains a papaya protease and a flavor protease with a mass ratio of 1.5 to 3:1; vitamin C which is 1-6 percent of the total amount of the enzyme is also added in the enzymolysis. The preparation method disclosed by the invention has the advantages that the momordica charantia p-polypeptide purity is improved, and the momordica charantia p-polypeptide mouth feeling is improved.
Description
Technical field
The present invention relates to the preparation method of bitter gourd polypeptide lyophilized powder, belong to biomedicine technical field.
Background technology
Diabetes (diabetesmellitus, DM) it is one group of syndrome caused by environment and inherited genetic factors acting in conjunction, its basic pathology feature be insulin secretion definitely or relative deficiency, or peripheral tissues is to insulin insensitivity, cause based on carbohydrate metabolism disturbance, comprise a kind of systemic disease of fat, protein metabolism disorder. The multinomial Study of evidence based medicine such as the clinical trial (DCCT) of blood glucose control and complication, the perspective diabetes study of Britain (UKPDS) have made people the meaning of strengthening glycemic control be reached common understanding, blood glucose fluctuation is possible not only to the generation preventing or delaying chronic complicating diseases of diabetes, improve the quality of life of patient, and can greatly reduce the relevant medical expense of diabetes. But the blood glucose fluctuation rate of diabetic subject is lower in reality. Insulin injection is one of method for the treatment of diabetes, but finds in clinical treatment, and also there are the following problems for injection of insulin itself: (1) injection can only be reduced during blood sugar, change of blood sugar curve controlled blood sugar can not be pressed completely; (2) hypoglycemia is easily produced; (3) Vapor recovery unit of Regular Insulin is poor, and absorption difference reaches 52%; (4) complication can not effectively be controlled. Oral antidiabetic drug treatment diabetes are a kind of long-term treatment and maintenance process, but the simple target relying on orally-taken blood sugar reducing cannot reach intensive cure, mainly due to the raw resistance of long-term oral antidiabetic drug produce and what side reaction caused. Therefore, it is urgent for seeking more efficiently ofhypoglycemic medicine.
Bitter gourd polypeptide is a kind of pure natural plant ingredients, by activating Regular Insulin, activate insulin receptor, it is to increase Regular Insulin effective rate of utilization regulates blood sugar, thus achieves the object that blood sugar is expressed according to the human body normal physiological natural law, and is free from side effects. Bitter gourd polypeptide is a kind of safety for diabetic subject and high blood glucose crowd provide, the solution of lasting blood glucose regulation, alleviates people when using medicament adjusting blood sugar, a series of body and mind injury difficult problem brought to self.
Preparation method's prior art of bitter gourd polypeptide has been reported, as State Intellectual Property Office in publication number disclosed in 16 days March in 2011 be CN101985644A, name is called the patent of invention of " a kind of bitter gourd polypeptide and its preparation method and application ", this patent is by adopting the direct enzymolysis Fructus Momordicae charantiae powder of papoid, then through sedimentation, filter, collect the object that filtrate realizes extracting, the raw material that the method uses is Fructus Momordicae charantiae powder, namely it is comprise bitter melon seed and balsam pear meat, but bitter gourd polypeptide is present in bitter melon seed, the extraction of polypeptide is carried out in the lump after balsam pear meat and bitter melon seed being pulverized, can greatly improve cost, and product impurity content height, the purity of bitter gourd polypeptide is low, corresponding result of use is also poor.
Summary of the invention
The present invention proposes the preparation method of a kind of bitter gourd polypeptide lyophilized powder, after selecting papoid and flavor protease to mix with specified proportion, raw material is hydrolyzed, while macro-molecular protein being hydrolyzed into small protein, improve the mouthfeel of bitter gourd polypeptide so that it is be more suitable in food, medicine; Additionally by steps such as degreasing, alkali-soluble, acid are heavy, improve the purity of bitter gourd polypeptide further, thus improve product quality.
In order to realize foregoing invention object, the technical scheme of the present invention is as follows:
The preparation method of bitter gourd polypeptide lyophilized powder, it is characterized in that: comprise that raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid are heavy, centrifugal, washing, lyophilize step, the enzyme of described enzymolysis use for mass ratio be papoid and the flavor protease of 1.5��3:1; Described enzymolysis step also adds the vitamins C of enzyme total amount 1��6%.
Further, described raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 20��40min in baking oven, grinding, crosses 40��60 order sieves, collect screen underflow and carry out sterilising treatment.
Described degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 10��15 times, stirs after evenly, leaves standstill 30��60min, refilter.
Described alkali-soluble is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 5��8h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 12��15%.
The temperature of described enzymolysis is 38��45 DEG C, and pH value is 5.0��6.0.
Described acid sink be point to the solution after enzymolysis adds and liquor capacity than the vitriol oil for 1:100, leave standstill 10��20min after stirring; The volumetric concentration of the described vitriol oil is more than 98%.
Described centrifugal rotating speed is 10000��11000 revs/min, and centrifugation time is 10��20min.
Described washing acid sinks the precipitation obtained add acetone and carry out washing 2��4 times.
Described lyophilize refers to that the temperature that is deposited in after by washing is-20��-10 DEG C, and pressure is dry 1��2h under 5��8Pa.
The useful effect of the present invention is as follows:
(1) raw material is hydrolyzed by the present invention after selecting papoid and flavor protease to mix with specified proportion, macro-molecular protein is hydrolyzed into small protein, bitter gourd polypeptide is obtained after being further purified, Flavor Prccursors can be hydrolyzed by flavor protease wherein, thus discharge flavour substances, strengthen and improve the local flavor of finished product, and the bitter taste of peptide can be controlled, its mouthfeel is made to be more suitable for food, medicine, easily accepted by human consumer, the present invention also adds the vitamins C of specified proportion, enzyme is had activation by vitamins C, thus reduce the actual usage quantity of enzyme, reduce production cost, additionally by the design of the steps such as degreasing, alkali-soluble, acid is heavy, and the selection of parameter, improve the purity of bitter gourd polypeptide further, thus improve product quality and ensure pharmaceutical activity.
(2) the present invention first carries out drying and processing at bitter melon seed, until bitter melon seed hardening crisp after, then grind, grinding effect and production efficiency can be improved.
(3) the centrifugal precipitation obtained of the present invention, washs further through adding acetone, the plurality of impurities in precipitation is separated, and improves the purity of bitter gourd polypeptide further.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 1.5:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 1%.
Embodiment 2
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 3:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 6%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 20min in baking oven, grinding, crosses 40 order sieves, collect screen underflow and carry out sterilising treatment.
Embodiment 3
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 2:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 3%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 40min in baking oven, grinding, crosses 60 order sieves, collect screen underflow and carry out sterilising treatment; Degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 10 times, stirs after evenly, leaves standstill 30min, refilter.
Embodiment 4
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 3:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 3%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 30min in baking oven, grinding, crosses 50 order sieves, collect screen underflow and carry out sterilising treatment; Degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 15 times, stirs after evenly, leaves standstill 60min, refilter.
Described alkali-soluble is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 5h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 12%.
Embodiment 5
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 1.5:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 5%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 25min in baking oven, grinding, crosses 50 order sieves, collect screen underflow and carry out sterilising treatment; Degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 12 times, stirs after evenly, leaves standstill 40min, refilter.
The alkali-soluble of the present embodiment is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 8h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 15%.
The total amount of the enzyme that the present embodiment uses for obtain after alkali-soluble the 0.5% of the weight of supernatant liquor; The temperature of enzymolysis is 38 DEG C, and pH value is 5.0.
Embodiment 6
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 2:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 3%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 35min in baking oven, grinding, crosses 50 order sieves, collect screen underflow and carry out sterilising treatment; Degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 13 times, stirs after evenly, leaves standstill 45min, refilter.
The alkali-soluble of the present embodiment is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 6h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 12%.
The total amount of the enzyme that the present embodiment uses for obtain after alkali-soluble the 2% of the weight of supernatant liquor; The temperature of enzymolysis is 45 DEG C, and pH value is 6.0; Acid is heavy is add with liquor capacity than the vitriol oil for 1:100 in the solution after pointing to enzymolysis, leaves standstill 10min after stirring; The volumetric concentration of the described vitriol oil is 98%.
Embodiment 7
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 3:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 4%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 28min in baking oven, grinding, crosses 60 order sieves, collect screen underflow and carry out sterilising treatment; Degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 14 times, stirs after evenly, leaves standstill 45min, refilter.
The alkali-soluble of the present embodiment is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 6h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 12%.
The total amount of the enzyme that the present embodiment uses for obtain after alkali-soluble the 3% of the weight of supernatant liquor; The temperature of enzymolysis is 42 DEG C, and pH value is 6.0; Acid is heavy is add with liquor capacity than the vitriol oil for 1:100 in the solution after pointing to enzymolysis, leaves standstill 20min after stirring; The volumetric concentration of the described vitriol oil is 98.5%; Centrifugal rotating speed is 10000 revs/min, and centrifugation time is 10min, gets precipitation.
Embodiment 8
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 3:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 6%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 40min in baking oven, grinding, crosses 60 order sieves, collect screen underflow and carry out sterilising treatment; Degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 14 times, stirs after evenly, leaves standstill 60min, refilter.
The alkali-soluble of the present embodiment is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 6h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 15%.
The temperature of the enzymolysis of the present embodiment is 40 DEG C, and pH value is 5.0; Acid is heavy is add with liquor capacity than the vitriol oil for 1:100 in the solution after pointing to enzymolysis, leaves standstill 20min after stirring; The volumetric concentration of the described vitriol oil is more than 98%; Centrifugal rotating speed is 11000 revs/min, and centrifugation time is 20min, gets precipitation.
The washing of the present embodiment be acid is sunk the precipitation obtained add acetone carry out washing 4 times.
Embodiment 9
The preparation method of bitter gourd polypeptide lyophilized powder, comprise raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid heavy, centrifugal, washing, lyophilize step, described enzymolysis use enzyme for mass ratio be 3:1 papoid and flavor protease; Described enzymolysis step also adds the vitamins C of enzyme total amount 6%.
In the present embodiment, raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, and after seed benevolence is dried 40min in baking oven, grinding, crosses 60 order sieves, collect screen underflow and carry out sterilising treatment, and sterilization method is pasteurization; Degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 14 times, stirs after evenly, leaves standstill 40min, refilter.
The alkali-soluble of the present embodiment is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 6h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 15%.
The total amount of the enzyme that the present embodiment uses for obtain after alkali-soluble the 2.5% of the weight of supernatant liquor; The temperature of enzymolysis is 40 DEG C, and pH value is 5.0; Acid is heavy is add with liquor capacity than the vitriol oil for 1:100 in the solution after pointing to enzymolysis, leaves standstill 15min after stirring; The volumetric concentration of the described vitriol oil is more than 98%; Centrifugal rotating speed is 10000 revs/min, and centrifugation time is 12min, gets precipitation.
The washing of the present embodiment be acid is sunk the precipitation obtained add acetone carry out washing 2 times.
Embodiment 10
The difference of the present embodiment and embodiment 9 is: acid is sunk the precipitation obtained and added acetone and carry out washing 3 times by washing.
The lyophilize condition of the present embodiment is that temperature is-20 DEG C, and pressure is dry 1h under 5Pa.
Embodiment 11
The lyophilize condition of the present embodiment is that temperature is-10 DEG C, and pressure is dry 2h under 8Pa.
The above is only the better embodiment of the present invention, and the present invention not does any restriction in form, every above embodiment is done according to the technical spirit of the present invention any simple modification, equivalent variations, all fall within protection scope of the present invention.
Claims (10)
1. the preparation method of bitter gourd polypeptide lyophilized powder, it is characterized in that: comprise that raw materials pretreatment, degreasing, alkali-soluble, enzymolysis, acid are heavy, centrifugal, washing, lyophilize step, the enzyme of described enzymolysis use for mass ratio be papoid and the flavor protease of 1.5��3:1; Described enzymolysis step also adds the vitamins C of enzyme total amount 1��6%.
2. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 1, it is characterised in that: described raw materials pretreatment refers to after being shelled by bitter melon seed, selects without the seed benevolence rotted, after seed benevolence is dried 20��40min in baking oven, grinding, crosses 40��60 order sieves, collects screen underflow and carry out sterilising treatment.
3. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 1, it is characterised in that: described degreasing adds, in the bitter melon seed powder after pointing to sterilizing, the acetone that weight is Fructus Momordicae charantiae powder weight 10��15 times, stirs after evenly, leaves standstill 30��60min, refilter.
4. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 1, it is characterised in that: described alkali-soluble is the ammoniacal liquor adding bitter melon seed weight 10 times in the bitter melon seed after pointing to degreasing, stirs after evenly, leaves standstill 5��8h, get supernatant liquor for subsequent use; The volumetric concentration of described ammoniacal liquor is 12��15%.
5. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 1, it is characterised in that: the temperature of described enzymolysis is 38��45 DEG C, and pH value is 5.0��6.0.
6. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 5, it is characterised in that: described acid sink be solution after pointing to enzymolysis adds and liquor capacity than the vitriol oil for 1:100, leave standstill 10��20min after stirring; The volumetric concentration of the described vitriol oil is more than 98%.
7. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 1, it is characterised in that: described centrifugal rotating speed is 10000��11000 revs/min, and centrifugation time is 10��20min, gets precipitation.
8. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 6, it is characterised in that: described washing acid sinks the precipitation obtained add acetone and carry out washing 2��4 times.
9. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 1, it is characterised in that: described lyophilize refers to that the temperature that is deposited in after by washing is-20��-10 DEG C, and pressure is dry 1��2h under 5��8Pa.
10. the preparation method of bitter gourd polypeptide lyophilized powder as claimed in claim 2, it is characterised in that: described sterilization method is pasteurization.
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Cited By (5)
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| CN109700024A (en) * | 2018-12-13 | 2019-05-03 | 深圳市淳睿贸易有限公司 | Balsam pear peptide combinations |
| CN109757642A (en) * | 2019-03-21 | 2019-05-17 | 安徽农业大学 | A kind of preparation method of balsam pear solid beverage |
| CN111171112A (en) * | 2020-03-17 | 2020-05-19 | 广西壮族自治区农业科学院 | Enzymatic extraction method of bitter melon seed protein |
| CN111850074A (en) * | 2020-07-10 | 2020-10-30 | 陕西慧科植物开发有限公司 | Preparation method and application of bitter gourd polypeptide |
| CN112618406A (en) * | 2020-12-30 | 2021-04-09 | 杭州娃哈哈科技有限公司 | Bitter gourd seed peptide powder with free radical scavenging effect, and preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109700024A (en) * | 2018-12-13 | 2019-05-03 | 深圳市淳睿贸易有限公司 | Balsam pear peptide combinations |
| CN109757642A (en) * | 2019-03-21 | 2019-05-17 | 安徽农业大学 | A kind of preparation method of balsam pear solid beverage |
| CN111171112A (en) * | 2020-03-17 | 2020-05-19 | 广西壮族自治区农业科学院 | Enzymatic extraction method of bitter melon seed protein |
| CN111850074A (en) * | 2020-07-10 | 2020-10-30 | 陕西慧科植物开发有限公司 | Preparation method and application of bitter gourd polypeptide |
| CN112618406A (en) * | 2020-12-30 | 2021-04-09 | 杭州娃哈哈科技有限公司 | Bitter gourd seed peptide powder with free radical scavenging effect, and preparation method and application thereof |
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