CN1363681A - 源于脐带血的活化淋巴细胞、含有所述淋巴细胞作为主要成分的制剂和制备所述制剂的方法与成套器具 - Google Patents
源于脐带血的活化淋巴细胞、含有所述淋巴细胞作为主要成分的制剂和制备所述制剂的方法与成套器具 Download PDFInfo
- Publication number
- CN1363681A CN1363681A CN01140404A CN01140404A CN1363681A CN 1363681 A CN1363681 A CN 1363681A CN 01140404 A CN01140404 A CN 01140404A CN 01140404 A CN01140404 A CN 01140404A CN 1363681 A CN1363681 A CN 1363681A
- Authority
- CN
- China
- Prior art keywords
- cord blood
- lymphocytes
- derived
- umbilical cord
- activated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004698 lymphocyte Anatomy 0.000 title claims abstract description 118
- 210000004700 fetal blood Anatomy 0.000 title claims abstract description 106
- 238000002360 preparation method Methods 0.000 title claims description 44
- 238000000034 method Methods 0.000 title claims description 26
- 210000004027 cell Anatomy 0.000 claims abstract description 40
- 102000000588 Interleukin-2 Human genes 0.000 claims abstract description 25
- 108010002350 Interleukin-2 Proteins 0.000 claims abstract description 25
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 25
- 230000002062 proliferating effect Effects 0.000 claims abstract description 23
- 210000001616 monocyte Anatomy 0.000 claims abstract description 16
- 208000015181 infectious disease Diseases 0.000 claims abstract description 13
- 238000000338 in vitro Methods 0.000 claims abstract description 11
- 210000005087 mononuclear cell Anatomy 0.000 claims abstract description 8
- 238000010521 absorption reaction Methods 0.000 claims abstract description 6
- 210000000130 stem cell Anatomy 0.000 claims abstract description 6
- 210000000056 organ Anatomy 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims description 19
- 230000009385 viral infection Effects 0.000 claims description 15
- 238000009472 formulation Methods 0.000 claims description 12
- 208000036142 Viral infection Diseases 0.000 claims description 11
- 230000003213 activating effect Effects 0.000 claims description 11
- 208000035473 Communicable disease Diseases 0.000 claims description 10
- 230000001737 promoting effect Effects 0.000 claims description 4
- 238000011282 treatment Methods 0.000 abstract description 11
- 230000002265 prevention Effects 0.000 abstract description 10
- 206010061819 Disease recurrence Diseases 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 19
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 14
- 201000010099 disease Diseases 0.000 description 13
- 239000002609 medium Substances 0.000 description 11
- 230000035755 proliferation Effects 0.000 description 10
- 239000000427 antigen Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 208000032839 leukemia Diseases 0.000 description 7
- 239000000825 pharmaceutical preparation Substances 0.000 description 7
- 239000007790 solid phase Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000002054 transplantation Methods 0.000 description 6
- 241000725303 Human immunodeficiency virus Species 0.000 description 5
- 239000012980 RPMI-1640 medium Substances 0.000 description 5
- 230000000259 anti-tumor effect Effects 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000002504 physiological saline solution Substances 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 4
- 102000006354 HLA-DR Antigens Human genes 0.000 description 4
- 108010058597 HLA-DR Antigens Proteins 0.000 description 4
- 238000010322 bone marrow transplantation Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 208000024908 graft versus host disease Diseases 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 210000003954 umbilical cord Anatomy 0.000 description 4
- 206010061598 Immunodeficiency Diseases 0.000 description 3
- 206010021450 Immunodeficiency congenital Diseases 0.000 description 3
- 208000026935 allergic disease Diseases 0.000 description 3
- 210000001185 bone marrow Anatomy 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 3
- 239000003018 immunosuppressive agent Substances 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 238000001990 intravenous administration Methods 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 208000011580 syndromic disease Diseases 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 2
- 208000029483 Acquired immunodeficiency Diseases 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 206010010099 Combined immunodeficiency Diseases 0.000 description 2
- 241000701022 Cytomegalovirus Species 0.000 description 2
- 206010011831 Cytomegalovirus infection Diseases 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 2
- 208000029462 Immunodeficiency disease Diseases 0.000 description 2
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007813 immunodeficiency Effects 0.000 description 2
- 229960003444 immunosuppressant agent Drugs 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 208000030507 AIDS Diseases 0.000 description 1
- -1 AIM-V Substances 0.000 description 1
- 208000035285 Allergic Seasonal Rhinitis Diseases 0.000 description 1
- APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 1
- 229920001342 Bakelite® Polymers 0.000 description 1
- 208000003174 Brain Neoplasms Diseases 0.000 description 1
- 206010061041 Chlamydial infection Diseases 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 240000005109 Cryptomeria japonica Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010015108 Epstein-Barr virus infection Diseases 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 208000022072 Gallbladder Neoplasms Diseases 0.000 description 1
- 208000001204 Hashimoto Disease Diseases 0.000 description 1
- 208000030836 Hashimoto thyroiditis Diseases 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 241000701085 Human alphaherpesvirus 3 Species 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 206010021460 Immunodeficiency syndromes Diseases 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 1
- 206010028470 Mycoplasma infections Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 206010029719 Nonspecific reaction Diseases 0.000 description 1
- 206010033128 Ovarian cancer Diseases 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 208000031845 Pernicious anaemia Diseases 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 108700017801 Purine Nucleoside Phosphorylase Deficiency Proteins 0.000 description 1
- 208000015634 Rectal Neoplasms Diseases 0.000 description 1
- 108010076622 SS-A antigen Proteins 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- 206010048908 Seasonal allergy Diseases 0.000 description 1
- 206010054979 Secondary immunodeficiency Diseases 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000024313 Testicular Neoplasms Diseases 0.000 description 1
- 206010057644 Testis cancer Diseases 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 208000024780 Urticaria Diseases 0.000 description 1
- 208000002495 Uterine Neoplasms Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 201000009628 adenosine deaminase deficiency Diseases 0.000 description 1
- 230000000735 allogeneic effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 1
- 229960003942 amphotericin b Drugs 0.000 description 1
- 230000000118 anti-neoplastic effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 238000002617 apheresis Methods 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 208000036556 autosomal recessive T cell-negative B cell-negative NK cell-negative due to adenosine deaminase deficiency severe combined immunodeficiency Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000004637 bakelite Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000003327 cancerostatic effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 201000010175 gallbladder cancer Diseases 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 229940125721 immunosuppressive agent Drugs 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 208000030603 inherited susceptibility to asthma Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000003071 memory t lymphocyte Anatomy 0.000 description 1
- 229960003085 meticillin Drugs 0.000 description 1
- 239000003226 mitogen Substances 0.000 description 1
- 206010028417 myasthenia gravis Diseases 0.000 description 1
- 210000004296 naive t lymphocyte Anatomy 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 201000002528 pancreatic cancer Diseases 0.000 description 1
- 208000008443 pancreatic carcinoma Diseases 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 208000001917 purine nucleoside phosphorylase deficiency Diseases 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 206010038038 rectal cancer Diseases 0.000 description 1
- 201000001275 rectum cancer Diseases 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 201000005404 rubella Diseases 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000011476 stem cell transplantation Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 201000000596 systemic lupus erythematosus Diseases 0.000 description 1
- 201000003120 testicular cancer Diseases 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 206010046766 uterine cancer Diseases 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
- A61K40/41—Vertebrate antigens
- A61K40/42—Cancer antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/515—CD3, T-cell receptor complex
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP368287/2000 | 2000-12-04 | ||
| JP2000368287A JP4917201B2 (ja) | 2000-12-04 | 2000-12-04 | 臍帯血移植後の腫瘍および各種感染症の予防・治療用製剤、臍帯血移植後の移植臍帯血幹細胞の生着促進用製剤、臍帯血移植後の腫瘍および各種感染症の予防・治療用製剤ならびに臍帯血移植後の移植臍帯血幹細胞の生着促進用製剤の製造方法。 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1363681A true CN1363681A (zh) | 2002-08-14 |
Family
ID=18838567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN01140404A Pending CN1363681A (zh) | 2000-12-04 | 2001-12-04 | 源于脐带血的活化淋巴细胞、含有所述淋巴细胞作为主要成分的制剂和制备所述制剂的方法与成套器具 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US6692958B2 (enExample) |
| EP (1) | EP1211311A3 (enExample) |
| JP (1) | JP4917201B2 (enExample) |
| CN (1) | CN1363681A (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1798834B (zh) * | 2003-06-03 | 2013-06-19 | 株式会社淋巴技术 | 使造血干细胞移植后生长稳定的组合物及获取该组合物的试剂盒和多克隆抗体及其制备方法 |
| CN104204195A (zh) * | 2012-04-10 | 2014-12-10 | 株式会社淋巴技术 | 以记忆t细胞为主要成分的淋巴细胞群的制造方法 |
| CN114438027A (zh) * | 2022-02-28 | 2022-05-06 | 浙江卫未生物医药科技有限公司 | 一种脐带血淋巴细胞制剂的制备方法 |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7572631B2 (en) | 2000-02-24 | 2009-08-11 | Invitrogen Corporation | Activation and expansion of T cells |
| JP4562353B2 (ja) * | 2002-04-08 | 2010-10-13 | 株式会社リンフォテック | Ｈｌａ一致他人由来活性化リンパ球を含有してなる腫瘍・感染症および自己免疫疾患の予防・治療用製剤 |
| WO2005090977A1 (en) | 2004-03-12 | 2005-09-29 | Exelixis, Inc | C20orf23 as modifier of the igfr pathway and methods of use |
| US20080267972A1 (en) * | 2005-02-10 | 2008-10-30 | Invitrogen Corporation | Donor Lymphocyte Infusion of T Cells For the Treatment of Cancer |
| JP2007055906A (ja) * | 2005-08-22 | 2007-03-08 | Lymphotec:Kk | 腫瘍犬の治療法、その治療用製剤、腫瘍犬治療用細胞の凍結保存方法 |
| RU2462511C2 (ru) * | 2006-08-01 | 2012-09-27 | Ариджен Фармасьютикалз, Инк. | Способ пролиферации клеток lak |
| JP5596007B2 (ja) * | 2011-12-09 | 2014-09-24 | 保幸 黒岩 | 細胞培養方法および培養装置 |
| CN113952475B (zh) * | 2021-09-27 | 2025-03-11 | 中国人民解放军陆军军医大学 | 淋巴细胞和介导crispr系统的纳米复合物联合在制备治疗宫颈癌药物中的应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GR1001034B (el) * | 1989-07-21 | 1993-03-31 | Ortho Pharma Corp | Μεθοδος διεγερσης του πολλαπλασιασμου λεμφοκυτταρων περιφερειακου αιματος. |
| IL112969A (en) * | 1994-03-17 | 2001-05-20 | Baxter Int | Pharmaceutical compositions for the treatment of cancer comprising allogenic lymphocytes or their combination with a t-cell activator |
| US5773224A (en) * | 1996-02-12 | 1998-06-30 | Grandics; Peter | Immunoselection system for cell elution |
| DE69738997D1 (de) * | 1996-07-15 | 2008-10-30 | Lymphotec Inc | Mittel zur heilung bzw. vorbeugung viraler infektionen, verfahren zu deren herstellung sowie verfahren zur vorbeugung bzw. heilung viraler infektionen |
| JP4389039B2 (ja) * | 1998-04-09 | 2009-12-24 | 株式会社リンフォテック | アロジェニックな活性化ｃｄ４陽性細胞を主成分とする医薬組成物、およびその製造方法、ならびに該医薬組成物調製用キット |
-
2000
- 2000-12-04 JP JP2000368287A patent/JP4917201B2/ja not_active Expired - Fee Related
-
2001
- 2001-12-03 EP EP01310091A patent/EP1211311A3/en not_active Ceased
- 2001-12-04 US US10/003,539 patent/US6692958B2/en not_active Expired - Lifetime
- 2001-12-04 CN CN01140404A patent/CN1363681A/zh active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1798834B (zh) * | 2003-06-03 | 2013-06-19 | 株式会社淋巴技术 | 使造血干细胞移植后生长稳定的组合物及获取该组合物的试剂盒和多克隆抗体及其制备方法 |
| CN104204195A (zh) * | 2012-04-10 | 2014-12-10 | 株式会社淋巴技术 | 以记忆t细胞为主要成分的淋巴细胞群的制造方法 |
| CN104204195B (zh) * | 2012-04-10 | 2017-07-18 | 株式会社淋巴技术 | 以记忆t细胞为主要成分的淋巴细胞群的制造方法 |
| CN114438027A (zh) * | 2022-02-28 | 2022-05-06 | 浙江卫未生物医药科技有限公司 | 一种脐带血淋巴细胞制剂的制备方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002171966A (ja) | 2002-06-18 |
| JP4917201B2 (ja) | 2012-04-18 |
| EP1211311A3 (en) | 2003-11-05 |
| EP1211311A2 (en) | 2002-06-05 |
| US20020086063A1 (en) | 2002-07-04 |
| US6692958B2 (en) | 2004-02-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102268405B (zh) | 自体nk细胞体外活化扩增培养的方法及其专用培养基 | |
| CN108251365B (zh) | 免疫细胞培养基体系 | |
| HK1215860A1 (zh) | Nk细胞强化型血液制剂的制造方法 | |
| US20080014174A1 (en) | Method for treating tumors, infectious diseases, and autoimmune diseases with an activated HLA matching donor-originating lymphocyte | |
| Després et al. | CD34+ cell enrichment for autologous peripheral blood stem cell transplantation by use of the CliniMACs device | |
| CN111454903A (zh) | 免疫细胞体外培养、诱导、激活、冻存方法及其细胞库建立 | |
| CN1363681A (zh) | 源于脐带血的活化淋巴细胞、含有所述淋巴细胞作为主要成分的制剂和制备所述制剂的方法与成套器具 | |
| JP4389039B2 (ja) | アロジェニックな活性化ｃｄ４陽性細胞を主成分とする医薬組成物、およびその製造方法、ならびに該医薬組成物調製用キット | |
| CN106754704B (zh) | 免疫细胞体外诱导扩增的方法 | |
| Yannelli et al. | Enhancement of human lymphokine-activated killer cell cytolysis and a method for increasing lymphokine-activated killer cell yields to cancer patients | |
| Pörtner et al. | Landscape of Manufacturing Process of ATMP Cell | |
| CN108192865B (zh) | Nk细胞体外扩增方法及用于该方法的试剂盒 | |
| CN111718901B (zh) | 一种高活性t细胞体外培养试剂盒及培养方法 | |
| WO1991002050A1 (en) | Enhanced lak cell activation by treatment of human peripheral blood mononuclear cells with amino acid amides | |
| Beaujean | Methods of CD34+ cell separation: comparative analysis | |
| EP0227809A1 (en) | METHOD AND COMPOSITION FOR TREATING CANCER AND NON-MALIGNAL TUMORS. | |
| CN112662631A (zh) | 一种car-t细胞灌流培养方法 | |
| EP0348290B1 (en) | Method of producing activated killer monocytes and method of monitoring their tumoricidal acivity | |
| CN111849897B (zh) | 一种细胞因子诱导杀伤细胞的体外活化方法 | |
| CN111690607B (zh) | 一种高效杀伤细胞体外培养试剂盒及培养方法 | |
| CN115340981A (zh) | 用于脐带血cd34阳性造血干细胞体外扩增的培养基 | |
| CN110684731A (zh) | 一种nk细胞的体外扩增培养方法 | |
| RU2835686C2 (ru) | Способ получения клеточного препарата на основе активированных лимфоцитов из лейкоцитарных фильтров | |
| CN114958740B (zh) | 体外培养富集人nk细胞的方法 | |
| Wijaya et al. | Comparison of Maturation Stages of Natural Killer Cell Differentiation Culture from Cultured and Freshly Isolated Umbilical Cord Blood Hematopoietic Stem Cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |