CN1305522C - Rat acarpous medicine and its preparation method - Google Patents
Rat acarpous medicine and its preparation method Download PDFInfo
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- CN1305522C CN1305522C CNB2004100813978A CN200410081397A CN1305522C CN 1305522 C CN1305522 C CN 1305522C CN B2004100813978 A CNB2004100813978 A CN B2004100813978A CN 200410081397 A CN200410081397 A CN 200410081397A CN 1305522 C CN1305522 C CN 1305522C
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Abstract
The present invention provides a medicine for making mice sterile and a preparation method of the medicine, which is characterized in that natural components (castor oil and castor protein) which can make mice sterile are respectively separated and extracted from castor beans; after the castor oil and the castor protein are respectively microencapsulated by conventional materials, the microcapsules of castor seed oil and castor protein are used as raw medicine to be matched with anti-corrosive medicine and bait, and medicine of the present invention is formed. The medicine for making mice sterile comprises microcapsules of castor oil, microcapsules of castor protein, anti-corrosive medicine and bait. The preparation, processing, storage and performance of the medicine can be easily controlled. The present invention has the value and the significance of practical application.
Description
One. technical field
The present invention relates to a kind of rat acarpous medicine and preparation method thereof, belong to the plant source medication field of killing mouse.
Two. background technology
The plague of rats is a kind of global serious biological epidemics, and the control plague of rats remains the great technical barrier of scientific and technological circle.Passed through the war of very long people Mus, the major technique of the control plague of rats has physics method, chemical method, microbial method and ecological method etc. both at home and abroad at present.Controlling bandicoot with chemicals, is the most general method of current use.The characteristics of chemical deratization medicine are to use conveniently, and instant effect can the timely control action plague of rats when the big generation of the plague of rats.But because the recovery capacity of muroid population is strong, chemistry is killed shortcomings such as having " population bounce-back ", contaminated environment, natural enemy secondary poisoning, and sterile control has become one of focus of current muroid population quantity Control Study.Aspect muroid control, it is more potential than traditional poisoning method that mouse sterile has been generally acknowledged.
At present, rat acarpous medicine has chemical sterilant medicine, sterile vaccine and plant acarpous medicine etc.Give mammal, the particularly human a lot of present situation of bringing of toxic and side effects at life-time service chemistry contraceptive or acarpous medicine, seek easy to use, easy row, cost lower, be a difficult task to the more friendly novel plant acarpous medicine of environment, be again necessary and necessity.The natural plants acarpous medicine is the novel and environment amenable BIOLOGICAL CONTROL medicine of a class of the sterile control plague of rats, and its research and development is subjected to people's attention always.
Semen Ricini (Ricinus communis Linn) originates from African east, is one of the world ten big oil crop.China has long plantation history.Consult pertinent literature and analysis more as can be known, at present, though utilize castor bean as the existing report of the research of antifertility drug, mainly is that it is carried out simple application.As, how to use as the batching of antifertility drug or certain antifertility drug with the form of castor bean crude extract or direct ground product.Three the most approaching prior arts of technology contents are 1. Chinese patents 01114461.0 disclosed " infertile cream ", its principal character is to comprise medicine concentrated extracts such as Semen Persicae, Flos Carthami, Fructus Aurantii, Radix Achyranthis Bidentatae, Radix Angelicae Sinensis, the circle Radix Angelicae Dahuricae, Flos Magnoliae, Radix Gossypii, Radix Arnebiae (Radix Lithospermi), Radix Wikstroemae, the Rhizoma Anemarrhenae, Cortex Phellodendri, Semen Ziziphi Spinosae, Semen Ricini, muscone, leopard cat perfume (or spice) in existing medicinal rubber cream, with medical ointment plaster at umbilical part, can practise contraception (external application), castor bean only is a kind of composition wherein; 2. Chinese patent 03144033.9 disclosed " a kind of plaster for the treatment of hemiplegia hypertension and contraception ", its principal character can be made lady's external applied contraceptive for being that primary raw material adds activating blood removing stasis class medicines such as cyperus iria L. rhizoma scirpi, Rhizoma Curcumae with the Semen Ricini.These two patents are the human body external skin and paste the medical contraception medicine, and a kind of composition of castor bean in this medicine, with the castor bean crude extract or directly pulverize the back and be formed by directly mixing with other composition.Though these two the technology preparation method is cheap, technology is simple, the former medicine of this technology preparation is because of contained composition complexity, and effective ingredient is difficult to detect, and drug effect is not easy to stablize.Of paramount importancely be, Oleum Ricini, albumen are not produced respectively, and the two mixes mutually and is not easy micro encapsulation, thereby in the easy putrid and deteriorated inefficacy of nature; Simultaneously, the Semen Ricini crude extract of micro encapsulation or directly the ground product abnormal flavour is not strong causes the strong refusing to eat of field Mus easily and does not reach the effect purpose.3. the article " toxicity of castor bean antifertility action and clinical research " that publishes of foreign medical science Chinese medicine fascicle (calendar year 2001 the 23rd volume the 5th phase 286-287): its principal character is for extracting the female contraceptive agent line correlation research of going forward side by side from castor bean with petroleum ether.Castor bean ligroin extraction main component is Oleum Ricini (a no ricin), the people is had comparatively secular contraceptive efficacy, can be during the physiology of special selection in (4-5d after the menstruation determines no pregnancy and oral this ligroin extraction of menstrual disturbance patients 2.3-2.5g) make female contraceptive agent.But the effect that its antiearly pregnancy to conceived person, anti-implantation and midtrimester of pregnancy stop is significantly smaller than ricin (we have carried out related experiment relatively to this).Physiological situation between the female Mus individuality in field is widely different, can not artificially select to give during the specified physiology the female Mus in the field contraceptive of feeding, so the castor bean ligroin extraction is very limited to the antifertility action of field Mus.Simultaneously, this research is not carried out micro encapsulation to the Semen Ricini crude extract.
From castor bean, separate respectively, extract and all have Oleum Ricini, the ricin that makes Mus sterile, and respectively the two is carried out behind the micro encapsulation preparation again and become the research of rat acarpous medicine is not appeared in the newspapers.
Three. the content of invention
The objective of the invention is provides a kind of rat acarpous medicine and preparation method thereof at the deficiencies in the prior art, be characterized in from castor bean, separating respectively, extracting all have the natural component that makes Mus sterile: Oleum Ricini, ricin, after carrying out micro encapsulation respectively, be processed into bait, antiseptic organic assembling.
Purpose of the present invention realizes that by following proposal wherein said raw material umber is parts by weight except that specified otherwise.Its prescription is:
I. ricin microcapsule 10-50 part antiseptic 0.5-1.5 part
Bait 48-88 part
II. ricin microcapsule 10-25 part antiseptic 0.5-1.5 part
Oleum Ricini microcapsule 15-40 part bait 33-73 part
Wherein affiliated bait is a common prescription: sugar 5%, vegetable oil 3.4%, Sal 0.5%, monosodium glutamate 0.1%, Semen Maydis powder 38%, flour 17%, water 35%, Prussian blue 1%; Antiseptic is a sodium benzoate.
Wherein affiliated formula I is remarkable to the effect of antiearly pregnancy, anti-implantation and the midtrimester of pregnancy termination of female Mus.Formula I I adds micro encapsulation on the basis of formula I Oleum Ricini both significantly strengthened the effect that antiearly pregnancy, anti-implantation and the midtrimester of pregnancy of female Mus stops, simultaneously the female Mus that is in during the special physiological there is remarkable and secular contraceptive efficacy, so formula I I is better than formula I (we have carried out related experiment relatively to this) to Mus group's antifertility action.
Oleum Ricini and ricin carry out being not easy putrid and deteriorated inefficacy at nature behind the micro encapsulation among formula I and the formula I I, help that this medicine is resisted undesirable element in environment and for a long time, play a role efficiently.Simultaneously, the micro encapsulation of the two has shielded the abnormal flavour of Semen Ricini, is easy to be accepted by Mus, has improved the feed intake and the utilization rate of medicine.
The preparation method of this rat acarpous medicine is as follows:
Formula I: ricin microcapsule 10-50 part, antiseptic 0.5-1.5 part and bait 48-88 part are mixed fully, add an amount of water again and stir, send into particle pressing machine, be cut into required graininess, drying and packaging gets final product.
Being prepared as follows of wherein said ricin and microcapsule:
The residue that the extraction of ricin: 500g extracts behind the Oleum Ricini mixes homogenate in phosphate buffer.Homogenate was placed 24 hours for 4 ℃.Preparation supernatant centrifugal 30 minutes in 17000g is abandoned precipitation.In supernatant, add solid ammonium sulfate and make into 60% saturation.Placed centrifugal 40 minutes of 17000g 4 hours for 4 ℃.Precipitation dialysis 12 hours, centrifugal 15 minutes of 4 ℃ of 17000g.Get the supernatant cold drying, get solid ricin 110-122.5g, in 4 ℃ of refrigerators, preserve standby.Adopt mechanical agitation emulsion process (endogenous method) preparation sodium alginate ricin microcapsule: Semen Ricini extract and an amount of calcium carbonate of 6-60g are added in the sodium alginate soln of 1-2.5% concentration, evenly mixed.This mixture is joined in the liquid paraffin that contains 1%Span85, and stirring and emulsifying is even, adds the liquid paraffin that contains glacial acetic acid then, continues to stir 5min, leaves standstill 40min, filters and obtains calcium alginate gel beads.The gel beads that obtains is put in the liquid paraffin again, places 30 ℃ water-bath, add 100-600mlEudragit S
100Alcoholic solution stirred 2 hours.Filtering-depositing obtains Eudragit S
100The sodium alginate albumen microcapsule that coats is with the Tween80 aqueous solution cleaning of 1% volume fraction, dry getting final product.
Formula I I: ricin microcapsule 10-25 part, Oleum Ricini microcapsule 15-40 part, antiseptic 0.5-1.5 part and bait 33-73 part are mixed fully, add an amount of water again and stir, send into particle pressing machine, be cut into required graininess, drying and packaging gets final product.
Being prepared as follows of wherein said Oleum Ricini and microcapsule, ricin and microcapsule
A. the extraction of the preparation Oleum Ricini of Oleum Ricini and microcapsule: take by weighing castor bean 300 grams and fragmentation after shelling.Add 95% ethanol 450ml in the broken castor bean, at 50 ℃ of stirred in water bath 75h.The centrifugal 30min of 0 ℃ centrifuge.Preparation supernatant 380ml.Add the equivalent ether in the supernatant,, concentrate and remove soluble drug and get Oleum Ricini 120-150g at 60 ℃ stirred in water bath 24h.The preparation of Oleum Ricini microcapsule: the 50-80g Oleum Ricini is stirred under 45 ℃ of temperature, be distributed in the 500ml aqueous solution that contains the 55.5g gelatin.Under agitation add the 500ml aqueous solution that contains the 55.5g Radix Acaciae senegalis then, the warm water 4000ml that adds 45 ℃ is again diluted.Keeping PH=9, temperature is the aqueous solution 32g that adds the polyvinyl methyl ethermaleic anhydride-maleic anhydride multipolymer of 5% concentration under 45 ℃ of conditions in mixed liquor.Add 20% acetic acid in above-mentioned mixture system, when the solution pH value is transferred to PH=4.2, under agitation be cooled to below 10 ℃, it is 8-9 that reuse 20%NaOH transfers pH, stirs 15min, and it is an amount of to add 25% formaldehyde at last, stirs 1h under this temperature.Leave standstill, the supernatant that inclines washes microcapsule with water to nearly neutral getting final product.
B. the preparation of ricin and microcapsule: as above described in the formula I.
Used antiseptic, bait all is existing known technology among the present invention, can be used, and to be these auxiliary agents do not have a negative impact to the realization and good the obtaining of effect of the present invention of purpose of the present invention its precondition.
The present invention has following advantage:
1. have high-efficient and lasting:
We are to the important composition material of castor bean Mus sterillization medicine---and the two the single or mixed combination of Oleum Ricini and ricin and microcapsule product thereof is all tested, and its result shows that all Mus is had certain antifertility action.But the combined effect of Oleum Ricini and ricin is the most remarkable, and action time is the most lasting, and the compositions of the two organically combines the long-term lasting antibiosis fertility of efficient antibiosis fertility of the short-term of ricin and Oleum Ricini and reached best anti-Mus fertility effect.After the two is produced respectively and distinguishes micro encapsulation, be not easy putrid and deteriorated inefficacy, help that this medicine is resisted undesirable element in environment and for a long time, play a role efficiently at nature.Simultaneously, the micro encapsulation of the two has shielded the abnormal flavour of Semen Ricini, is easy to be accepted by Mus, has improved the feed intake and the utilization rate of medicine.
2. drug quality is easy to control, easy to use, easy row:
Medicine constituent of the present invention is comparatively simple, and important component is clear and definite, and detection method is simple, and drug quality is easy to control.Field row easy to use, easy.
3. environmentally friendly:
(1) noresidue
Another drawback of general chemical pesticide is exactly the toxic residue problem, and residual periodicity is long, and damaging range is wide, some raticide even can precipitate in organisms such as people and animals and accumulate.And castor bean Mus sterillization medicine is to utilize a kind of Mus sterillization material of natural plants through the scientific technology preparation, use amount is less, bandicoot is got food and all can reach sterile 1-2 time, environment is belonged to the low toxicity product, the nature biotechnology degraded is fast, come from nature, can there be the toxic residue problem in back to nature.
(2) produce the no three wastes
Generally, chemistry Mus medicine factory is existing and produce the pollution problem of the three wastes to surrounding more or less all, expended great amount of manpower, financial resources and material resources also is difficult to fundamentally address this problem for this reason, do not have the three exhausted problems of generation and produce castor bean Mus sterillization medicine: 1. producing remaining waste residue can be used for fermenting, the biogas that fermentation is produced is as utilization of energy, the fermentation medicinal residues are after carrying out the processing of organic proportioning and science with other fertilizer, can make first-class composite organic fertilizer, good development prospect and vast market are arranged; 2. the soluble drug after using can be recycled again; 3. there is not waste gas.
4. raw material sources are extensive, and are cheap
Semen Ricini originates from African east, is one of the world ten big oil crop.China has long plantation history.Semen Ricini wide accommodation, strong stress resistance, cultivation management condition are very not strict, and 80% above area, the whole nation can both be planted, thereby raw material sources are extensive, and is cheap.
5. extraction and pharmaceutical technology are easy, are easy to form large-scale production
The production technology of producing castor bean Mus sterillization medicine is also uncomplicated, and required equipment mainly contains extraction pot, concentrator, recovery tower, triple effect machine etc., and its production procedure is simple and clear, clear.Its technological process is: raw material-cleaning-pulverizing-immersion-backflow lixiviate-extraction-concentrate and micro encapsulation-mix-make medicine grain-drying and packaging-warehouse-in.The simple feasibility that has increased large-scale production and exploitation greatly of production process.
Four. specific implementation method
Below by embodiment the present invention is specifically described; be necessary to be pointed out that at this present embodiment only is used for the present invention is further specified; can not be interpreted as limiting the scope of the invention, the person skilled in the art in this field can make some nonessential improvement and adjustment to the present invention according to the content of the invention described above.
Embodiment 1:
With 25 parts of ricin microcapsules, antiseptic drug: 73.5 portions of (sugar 5% of 1.5 parts (sodium benzoate) and bait; vegetable oil 3.5%, Sal 0.5%, monosodium glutamate 0.1%; Semen Maydis powder 38%; flour 17%, water 35%, Prussian blue 1%) mix homogeneously; adding an amount of water again stirs; send into particle pressing machine, be cut into required graininess, drying and packaging both can.
Being prepared as follows of wherein said ricin and microcapsule:
The residue that the extraction of ricin: 500g extracts behind the Oleum Ricini mixes homogenate in phosphate buffer.Homogenate was placed 24 hours for 4 ℃.Preparation supernatant centrifugal 30 minutes in 17000g is abandoned precipitation.In supernatant, add solid ammonium sulfate and make into 60% saturation.Placed centrifugal 40 minutes of 17000g 4 hours for 4 ℃.Precipitation dialysis 12 hours, centrifugal 15 minutes of 4 ℃ of 17000g.Get the supernatant cold drying, get solid ricin 120.5g, in 4 ℃ of refrigerators, preserve standby.Adopt mechanical agitation emulsion process (endogenous method) preparation sodium alginate ricin microcapsule: Semen Ricini extract and an amount of calcium carbonate of 30g are added in the sodium alginate soln of 2% concentration 60ml, evenly mixed.This mixture is joined in the liquid paraffin that contains 1%Span85, and stirring and emulsifying is even, adds the liquid paraffin 60ml that contains the 300ul glacial acetic acid then, continues to stir 5min, leaves standstill 40min, filters and obtains calcium alginate gel beads.The gel beads that obtains is put in the liquid paraffin again, places 30 ℃ water-bath, add 300mlEudragit S
100Alcoholic solution stirred 2 hours.Filtering-depositing obtains Eudragit S
100The sodium alginate albumen microcapsule that coats is with the Tween80 aqueous solution cleaning of 1% volume fraction, dry getting final product.
Embodiment 2:
With 10 parts of ricin microcapsules, 35 parts of Oleum Ricini microcapsules, antiseptic drug 1.0 parts of (sodium benzoate) and 54.0 parts of (composition is with embodiment 1) mix homogeneously of bait; adding an amount of water again stirs; send into particle pressing machine, be cut into required graininess, drying and packaging gets final product.
Being prepared as follows of wherein said Oleum Ricini and microcapsule thereof:
The extraction of Oleum Ricini: take by weighing castor bean 300 grams and fragmentation after shelling.Add 95% ethanol 450ml in the broken castor bean, at 50 ℃ of stirred in water bath 75h.The centrifugal 30min of 0 ℃ centrifuge.Preparation supernatant 380ml.Add the equivalent ether in the supernatant,, concentrate and remove soluble drug and get Oleum Ricini 130g at 60 ℃ stirred in water bath 24h.The preparation of Oleum Ricini microcapsule: the 65g Oleum Ricini is stirred under 45 ℃ of temperature, be distributed in the 500ml aqueous solution that contains the 55.5g gelatin.Under agitation add the 500ml aqueous solution that contains the 55.5g Radix Acaciae senegalis then, the warm water 4000ml that adds 45 ℃ is again diluted.Keeping PH=9, temperature is the aqueous solution 32g that adds the polyvinyl methyl ethermaleic anhydride-maleic anhydride multipolymer of 5% concentration under 45 ℃ of conditions in mixed liquor.Add 20% acetic acid in above-mentioned mixture system, when the solution pH value is transferred to PH=4.2, under agitation be cooled to below 10 ℃, it is 8-9 that reuse 20%NaOH transfers pH, stirs 15min, and it is an amount of to add 25% formaldehyde at last, stirs 1h under this temperature.Leave standstill, the supernatant that inclines washes microcapsule with water to nearly neutral getting final product.
The preparation of wherein said ricin and microcapsule such as embodiment 1.
Embodiment 3: with 20 parts of ricin microcapsules, 20 parts of Oleum Ricini microcapsules, antiseptic drug 1.0 parts of (sodium benzoate) and 59.0 parts of (composition is with embodiment 1) mix homogeneously of bait; adding an amount of water again stirs; send into particle pressing machine, be cut into required graininess, drying and packaging gets final product.
The preparation of wherein said ricin and microcapsule such as embodiment 1.
The preparation of wherein said Oleum Ricini and microcapsule thereof such as embodiment 2.
Implement checking: Semen Ricini Mus apholate is to the antifertility action of Mus
One. method: experimental mouse is the house mouse of 25 ± 2g.
1. antiearly pregnancy, anti-midtrimester of pregnancy:
The male and female mice mated copulation by 2: 1, and check cloudy bolt morning every day, found that cloudy bolt day is pregnant the 1st day.Take out mice, raise respectively by the pregnancy period.Medicine feed mode: give sufficient pharmaceutical chemistry during every group of experimental mouse medicine feed and search for food naturally by it.Contrast the no pharmaceutical chemistry material of feeding.
Antiearly pregnancy: from pregnant plan 7 days, medicine feed bait 3 days (triplicate) continuously
Anti-midtrimester of pregnancy: from pregnant plan 11 days, medicine feed bait 3 days (triplicate) continuously.
Each experimental mice was all dissected after 1 administration in the end at interval in 1 day, observed the tire son, with downright bad free or absorb and leave the vestige person serves as effective with Uterus wall.The gestation of adding up every group respectively suppresses percentage rate.See Table 1 by probit method result of calculation.
2. anti-implantation: the male and female mice mated copulation by 2: 1, and check cloudy bolt morning every day, and the same day of finding cloudy bolt was as the 1st day of gestation.Mice is in the 1st, 2 and 3 day difference of gestation medicine feed bait.Respectively organize pregnant mouse number and average embryo number in the 12nd day statistics of gestation.Statistics gestation suppresses percentage rate (triplicate).Medicine feed mode: give sufficient pharmaceutical chemistry during every group of experimental mouse medicine feed and search for food naturally by it.Contrast the no pharmaceutical chemistry material of feeding.
3. long-term antifertility: mice is mated with male and female at 2: 1, do vaginal smear at m seq, see that sperm is d1 days, from the 2nd day continuous medicine feed bait 2 days, it is searched for food naturally and no longer farrows (mice all mates with male and female at 2: 1 therebetween) to matched group with relief, observes, adds up litter size (triplicate) at any time.Medicine feed mode: give sufficient pharmaceutical chemistry during every group of experimental mouse medicine feed and search for food naturally by it.Contrast the no pharmaceutical chemistry material of feeding.
Two. experimental result such as table 1: formula I (embodiment 1.) is remarkable to the effect of antiearly pregnancy, anti-implantation and the midtrimester of pregnancy termination of female Mus wherein.Formula I I (embodiment 2.3.) adds micro encapsulation on the basis of formula I Oleum Ricini both significantly strengthened the effect that antiearly pregnancy, anti-implantation and the midtrimester of pregnancy of female Mus stops, and simultaneously female Mus was had remarkable and secular contraceptive efficacy.
Observe to find simultaneously in the experiment that experimental mouse does not have significant difference to the feed feed intake of no pharmaceutical chemistry material of the feed intake of pharmaceutical chemistry and contrast Mus, show that the ricin of the Oleum Ricini of micro encapsulation and micro encapsulation has shielded the abnormal flavour of Semen Ricini, is easy to be accepted by Mus.
Table one Semen Ricini Mus apholate is to the antifertility action of mice
| Experiment content | Embodiment | ||
| 1 | 2. | 3 | |
| Antiearly pregnancy rate %# | 90.58 | 100 | 100 |
| Anti-implantation rate % | 79.41 | 91.56 | 89.23 |
| Anti-midtrimester of pregnancy rate % | 60.89 | 88.79 | 100 |
| Long-term antifertility rate % | 8.52 | 78.58 | 65.58 |
Annotate: the result who draws after # antiearly pregnancy rate, anti-implantation rate etc. are and compare.
Claims (8)
1. rat acarpous medicine is characterized in that containing from castor bean and separates, extracts and have the natural component that makes Mus sterile: ricin; Form joining behind the ricin micro encapsulation with antiseptic drug, bait organic assembling, the ricin actual content is 60-80% by weight percentage in the described ricin microcapsule, and capsule membrane material content is 20-40%; The main component of described capsule membrane material is sodium alginate and Eudragit S
100The separation and Extraction of ricin: the residue behind a certain amount of extraction Oleum Ricini mixes homogenate in buffer; Homogenate left standstill 24 hours for 4 ℃; The preparation supernatant is centrifugal, abandons precipitation; In supernatant, add solid ammonium sulfate and make into 60% saturation; Placed 4 hours for 4 ℃, centrifugal, precipitation dialysis 12 hours, centrifugal; Get the supernatant drying, get the solid ricin.
2. according to the described rat acarpous medicine of claim 1, it is characterized in that the prescription of this rat acarpous medicine is by weight:
Ricin microcapsule 10-50 part
Antiseptic 0.5-1.5 part
Bait 48-88 part
Wherein said bait is a common prescription: sugar 5%, vegetable oil 3.4%, Sal 0.5%, monosodium glutamate 0.1%, Semen Maydis powder 38%, flour 17%, water 35%, Prussian blue 1%; Antiseptic is a sodium benzoate.
3. according to claim 1 or 2 described rat acarpous medicines, it is characterized in that in this rat acarpous medicine, also containing the Oleum Ricini microcapsule; The separation and Extraction of described Oleum Ricini: it is a certain amount of and broken to take by weighing castor bean, add 95% ethanol in the broken castor bean, at 50 ℃ of stirred in water bath 75h, 0 ℃ centrifuge is centrifugal, the preparation supernatant, add the equivalent ether in the supernatant,, concentrate and remove soluble drug and get Oleum Ricini at 60 ℃ stirred in water bath 24h; Oleum Ricini, capsule film material actual amount are in the described Oleum Ricini microcapsule preparation process: the 65g Oleum Ricini is stirred under 45 ℃ of temperature, be distributed in the 500ml aqueous solution that contains the 55.5g gelatin, under agitation add the 500ml aqueous solution that contains the 55.5g Radix Acaciae senegalis then, the warm water 4000ml that adds 45 ℃ is again diluted, and is the aqueous solution 32g that adds polyvinyl methyl ethermaleic anhydride one maleic anhydride multipolymer of 5% concentration under 45 ℃ of conditions in mixed liquor keeping PH=9, temperature; The main component of capsule membrane material is gelatin, Radix Acaciae senegalis and polyvinyl methyl ethermaleic anhydride one maleic anhydride multipolymer.
4. according to the described rat acarpous medicine of claim 3, it is characterized in that the prescription of this rat acarpous medicine is by weight:
Ricin microcapsule 10-25 part antiseptic 0.5-1.5 part
Oleum Ricini microcapsule 15-40 part bait 33-73 part
Wherein said bait is a common prescription: sugar 5%, vegetable oil 3.4%, Sal 0.5%, monosodium glutamate 0.1%, Semen Maydis powder 38%, flour 17%, water 35%, Prussian blue 1%; Antiseptic is a sodium benzoate.
5. prepare the method for rat acarpous medicine as claimed in claim 2, it is characterized in that:
1. the extraction of the preparation ricin of ricin and microcapsule: centrifugal the abandoning of castor bean residue homogenate → water extract behind the extraction Oleum Ricini adds solid ammonium sulfate in precipitation → supernatant, and be centrifugal → the precipitation dialysis, centrifugal → supernatant drying → ricin; Adopt the mechanical agitation emulsion process to prepare sodium alginate ricin microcapsule: ricin and calcium carbonate add in the sodium alginate soln, mixed evenly → join in the liquid paraffin that contains Span85 → add the liquid paraffin that contains glacial acetic acid, filtrations → calcium alginate gel beads → be put in is in the liquid paraffin → adding Eudragit S
100Alcoholic solution, filtering-depositing → Eudragit S
100The sodium alginate albumen microcapsule that coats;
2. ricin microcapsule 10-50 part, antiseptic 0.5-1.5 part and bait 48-88 part are fully mixed, add an amount of water again and stir, send into particle pressing machine, be cut into required graininess, drying and packaging gets final product.
6. prepare the method for rat acarpous medicine as claimed in claim 4, it is characterized in that:
1. the preparation of Oleum Ricini and microcapsule, ricin and microcapsule
A. the extraction of the preparation Oleum Ricini of Oleum Ricini and microcapsule: castor bean fragmentation → ethanol extraction, the centrifugal → supernatant extraction → Oleum Ricini that adds diethyl ether; The preparation of Oleum Ricini microcapsule: Oleum Ricini and the aqueous solution that contains gelatin fully mix → join in the aqueous solution that contains Radix Acaciae senegalis → add an amount of acetic acid of the aqueous solution of polyvinyl methyl ethermaleic anhydride one maleic anhydride multipolymer → add respectively, NaOH adjust pH → add an amount of formaldehyde → Oleum Ricini microcapsule;
B. as above claim 5 is described in the preparation of ricin and microcapsule;
2. the preparation of this rat acarpous medicine: Oleum Ricini microcapsule 15-40 part, ricin microcapsule 10-25 part, antiseptic 0.5-1.5 part and bait 33-73 part are fully mixed; adding an amount of water again stirs; send into particle pressing machine, be cut into required graininess, drying and packaging gets final product.
7. according to the purposes of claim 1 or 3 described rat acarpous medicines: it is characterized in that the rat acarpous medicine that will contain ricin or Oleum Ricini is used to be developed as the medicine of control Mus.
8. according to the purposes of the described rat acarpous medicine of claim 7, wherein said medicine comprises tablet, slow releasing tablet, capsule, slow releasing capsule, soft capsule and pellet.
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| CN105994315B (en) * | 2016-05-26 | 2018-06-19 | 陕西省动物研究所 | A kind of pharmaceutical chemistry that can inhibit muroid breeding and preparation method thereof |
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2004
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5149528A (en) * | 1987-04-10 | 1992-09-22 | Zymogenetics, Inc. | Cytotoxic protein from Trichosanthes kirilowii |
| CN1195536A (en) * | 1997-04-07 | 1998-10-14 | 林丽平 | Plaster for curing hemiplegia, hypertension and contraception |
| CN1522740A (en) * | 1997-04-07 | 2004-08-25 | 林丽平 | Hemiplegia hypertension and contraceptive treating plaster |
| CN1322559A (en) * | 2001-05-15 | 2001-11-21 | 周霞轩 | Externally applied sterility plaster |
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