CN1278601C - Micro algae culturing liquid and its prepn process - Google Patents
Micro algae culturing liquid and its prepn process Download PDFInfo
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- CN1278601C CN1278601C CNB011341475A CN01134147A CN1278601C CN 1278601 C CN1278601 C CN 1278601C CN B011341475 A CNB011341475 A CN B011341475A CN 01134147 A CN01134147 A CN 01134147A CN 1278601 C CN1278601 C CN 1278601C
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- marine alga
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The present invention relates to a culture medium of a microbe, particularly to a microalgae cultivating solution and a preparation method thereof. The microalgae cultivating solution comprises extracting solutions containing macroscopic algae. The preparation method comprises the steps that (1), macroscopic algae are selected and are washed; (2), the macroscopic algae are crushed after thrown in a leaching solution; (3), algae powder or algae slurry is filtered and degummed, and clear liquid is obtained; (4), the clear liquid is concentrated; (5), the cultivating solution is obtained after the concentrated solution is processed by high temperature and high pressure. Compared with the prior art, the present invention has the advantaged of promotion of the growth of microalgae, rich component, and improved nutritional value and the physiological function of algea; simultaneously, the present invention is an all-natural cultivating solution without a chemical agent and has the advantages of no environmental pollution, simple preparation process, convenient implementation and low cost; the present invention is suitable for large-water cultivation of microalgae.
Description
Technical field
The present invention relates to the medium of a kind of microorganism, particularly a kind of micro algae culturing liquid and preparation method thereof.
Background technology
Existing micro algae culturing liquid all is chemical culture fluid, promptly by the chemical agent dissolves that contains various nutrient components, the culture fluid that is mixed and made into.When being applied to commercial production, this chemical culture fluid has the following disadvantages: (one) complicated component, the solution that has need instant preparation, waste time and energy, and operating process is loaded down with trivial details.When (two) being used to cultivate the little algae product of food-grade, some of them main component (as fertilizer nitrogen, chemical fertilizer phosphorus) does not meet the making food standard, has increased the wash-out operation in the product processing.Simultaneously, also can cause environmental pollution to a certain degree after this class culture fluid discharges.(3) lack the organic composite parts that contains in the natural water body, and these compositions have special dietary value, they play an important role in the process of the synthetic related substances of little algae.
Summary of the invention
The objective of the invention is to overcome weak point of the prior art, and complete micro algae culturing liquid of a kind of nutrition and preparation method thereof is provided.
Purpose of the present invention can realize through following scheme.
Micro algae culturing liquid comprises the extract of marine alga in its component.
Algae comprises marine alga and fresh water algae.The nutrition of algae is abundant, grows necessary various element (comprising trace element) except containing micro algae growth, also contains small-molecular peptides, sugar and somatotropin that little algae can absorb.Little algae of occurring in nature and marine alga are enjoyed common living space, and the product that marine alga is rotted or death is decomposed just becomes the nutrient source of little algae.Simultaneously, various nutritive elements have different structure proportions in different marine alga bodies, and this variation is embodied in little algae numerous in variety equally.So consider to utilize the marine alga preparation to be applicable to the micro algae culturing liquid of commercially producing little algae.
China marine site is vast, and mariculture is fast-developing, need get caught up in ocean bait---little algae of this development speed in a large number, and simultaneously, little algae still is human important function food source.
The marine algae resource of China is extremely abundant,, also contain the mineral matter (comprising trace element) of abundant ratio relative equilibrium and can improve the auxin of micro algae growth speed except contain the essential nitrogen of organism growth, phosphorus, carbon three big elements (oxygen obtains) from air with the micro algae culturing liquid of marine alga preparation.Because this culture fluid is the product of complete pure natural, can not produce in the use the unnecessary harm of water body, frond and human body, this is advocating environmental protection now and is safeguarding that meaning is very great aspect the human health.Experimental result proves that this micro algae culturing liquid with the marine alga preparation has than conventional chemical culture fluid better educates the algae effect.It can prolong its growth cycle when improving micro algae growth speed, thereby increases specific yield, reduces production costs.Also higher with its cultured microalgae unsaturated fatty acid content, therefore more eutrophy is worth.
Utilize the offcuts production in the marine alga process promptly to realize twice laid, enlarge the development of resources rate again, also can solve simultaneously and add the building site owing to stack environment and the hygienic issues that production rubbish causes for the culture fluid of micro algae growth.
Marine alga of the present invention can be single algae, also can be the compound algae of two or more algae.
Marine alga of the present invention still mixes to use all can obtain required micro algae culturing liquid no matter be independent use.How to arrange in pairs or groups and depend on the nutritional need of Object of Development, because its nutrition of being had of different algae is different.
The present invention is also that further this marine alga can be a sea-tangle, also can be laver, red hair algae.
Marine alga of the present invention mainly is the kind that body is big, output is high, and as sea-tangle, other algae mixes use with it as auxiliary element as laver, Lingula and red Mao Zao etc.
Sea-tangle is bigger a kind of of body in marine alga, and its fertility is strong, output is high, and price is low, and the source is wide, and therefore for the present invention, sea-tangle all is a good raw material.Raw material can adopt the remaining offcuts of processing sea-tangle product, also can adopt sea-tangle completely clean, that dry.At present, in composite material, red hair algae is best the replenishing of sea-tangle.
Also include extract of soybean in the component of the present invention, or Ginger P.E, or aloe extract, or garlic P.E.
Contain abundant nitrogen in the soybean, can provide micro algae growth breeding required nitrogenous source, thereby regulate N/P ratio in the culture fluid with it.Different little algaes have different requirements to the N/P ratio in the medium.Ginger, aloe etc. have special-effect to control breed variety damage by disease and insect, and its extract is added in the micro algae culturing liquid, again by little algae enrichment, can increase the disease resistance performance of algae food.
The preparation method of micro algae culturing liquid of the present invention is as follows:
1. select and clean marine alga;
2. marine alga is dropped into after the leaching liquor broken;
3. after algae end or algae slurry being filtered, removes photoresist clear liquid;
4. concentrate clear liquid;
5. culture fluid will promptly be got after the concentrate high temperature high pressure process.
This preparation method can also be specially following steps:
1. select and clean first marine alga and second marine alga;
2. with broken after first marine alga input leaching liquor, the algae of winning is starched;
3. with broken after second marine alga input leaching liquor, get second algae slurry;
4. mix first algae slurry and second algae slurry, get the mixed algae slurry;
5. mixed algae slurry is filtered, removes photoresist clear liquid;
6. concentrate clear liquid;
7. culture fluid will promptly be got after the concentrate high temperature high pressure process.
Be also that further first marine alga is a sea-tangle, second marine alga is a laver.
The fragmentation of marine alga is the physics fragmentation among this preparation technology, only need make the desired nutritional composition separate out just can, the necessary free from admixture (suspension) of the clear liquid of filtering can pass through fast qualitative filter paper.Impurity is unfavorable for the photosynthesis of algae, and it can make photosynthetic efficiency reduce greatly, is unfavorable for the normal growth of little algae.
In sum, the present invention compares to prior art and has following advantage; Can promote micro algae growth, component is abundant, helps to improve the nutritive value and the physiological function of frond, and it is the culture fluid of pure natural simultaneously, do not contain chemical reagent, free from environmental pollution, and preparation technology is simple, be convenient to implement, cost is low, is suitable for big water body and cultivates little algae.
Embodiment
Describe the present invention below in conjunction with embodiment.
Embodiment 1:
Adopt the raw material of the offcuts of sea-tangle processing as micro algae culturing liquid, its preparation method is as follows:
1. select the offcuts of sea-tangle processing, standby with the clean back of clear water rinsing;
2. sea-tangle is dropped into leaching liquor, with cracker it is carried out the physics fragmentation then, its nutrition is fully separated out;
3. broken gained sea-tangle homogenate is filtered, is removed photoresist clear liquid;
4. clear liquid being pressed desired concn concentrates;
5. concentrate is carried out promptly getting micro algae culturing liquid after the high temperature high pressure process.
Each main component content of just testing below in the gained micro algae culturing liquid compares with each the main component content in the seawater micro-algae culture medium commonly used in the world:
Each main component content compares (every liter of seawater) in table one, the micro algae culturing liquid
| Composition | Inorganic compounding * | The sea-tangle dilution ** |
| N P Cl Ca Co Cu Fe Hg K Mg Mn Mo Na Si Zn | 1.24×10 -3％ 1.12×10 -4％ 0.13×10 -3％ - 2.48×10 -3mg/L 2.5×10 -3mg/L 0.65mg/L - - - 5.0×10 -2mg/L 2.5×10 -3mg/L 2.1×10 -3％ - 5.0×10 -3mg/L | 0.5×10 -3％ 1.2×10 -4％ 2.8×10 -3％ 1.60mg/kg 4.3×10 -3mg/kg ＜0.5×10 -3mg/kg 0.1mg/kg ＜5×10 -4mg/kg 2.5×10 -3％ 2.07mg/kg 0.26×10 -2mg/kg 7.0×10 -3mg/kg 1.3×10 -3％ 6.0×10 -3mg/kg 2.8×10 -3mg/kg |
*: the f/2 medium sees Table two.
*: dilute 100 times, the stoste testing result is seen Fujian Province Institute of Analysis examining report.
Table two, seawater micro-algae culture medium (the Guillard ﹠amp that uses always in the world; Ryther 1962, Guillard1975), and every liter of seawater content:
| Quantity | Compound | Stoste |
| 1.0ml 1.0ml 1.0ml 0.5ml | NaNO 3 NaH 2PO 4.H 2O f/2 trace element f/2 vitamin | 75.0mg/L d H 2O 5.0mg/L d H 2O sees Table three (summaries) |
Table three, f/2 trace element (Guillard ﹠amp; Ryther 1962, and Guillard 1975), every liter of distilled water content:
| Quantity | Compound | Stoste |
| 3.15g 4.36g 1.0ml 1.0ml 1.0ml 1.0ml 1.0ml | FeCl 3.6H 2O Na 2EDTA.2H 2O CuSO 4.5H 2O Na 2MoO 4.2H 2O ZnSO 4.7H 2O CoCl 2.6H 2O MnCl 2.4H 2O | - - 9.8g/L d H 2O 6.3g/L d H 2O 22.0g/L d H 2O 10.0g/L d H 2O 180.0g/L d H 2O |
Shown in table one, table two and table three, with the micro algae culturing liquid of marine alga preparation except containing the essential nitrogen of organism growth, phosphorus, carbon three big elements, the trace element that also contains abundant ratio relative equilibrium, some is to the content of the favourable main component of micro algae growth even than also enriching in the seawater micro-algae culture medium commonly used in the world.
Most preferred embodiment:
Adopt offcuts, laver and the Chinese aloe leaf of the sea-tangle processing raw material as micro algae culturing liquid, its preparation method is as follows:
1. select the offcuts and the laver of sea-tangle processing, standby with the clean back of clear water rinsing;
2. sea-tangle is dropped into leaching liquor, with cracker it is carried out the physics fragmentation then, its nutrition is fully separated out, get kelp paste;
3. laver is dropped into leaching liquor, with cracker it is carried out the physics fragmentation then, its nutrition is fully separated out, get the laver slurry;
4. mix kelp paste and laver slurry, get the mixed algae slurry;
5. mixed algae slurry is filtered, removes photoresist clear liquid;
6. clear liquid being pressed desired concn concentrates;
7. concentrate is carried out getting sea-tangle-laver micro algae culturing liquid after the high temperature high pressure process;
8. squeeze its juice after Chinese aloe leaf is sterilized at normal temperatures, filter;
9. fresh asparagus juice clear liquid is put 60 ℃ of water-baths, make wherein most of enzyme deactivation;
10. the asparagus juice that will handle promptly gets sea-tangle-laver-aloe micro algae culturing liquid in 8～20% the ratio adding sea-tangle laver micro algae culturing liquid.
The purpose that adds asparagus juice is in order to strengthen the performance of the prevention and elimination of disease and pests of little algae in the micro algae culturing liquid.
Claims (8)
1. micro algae culturing liquid is characterized in that, comprises the extract of marine alga in its component.
2. micro algae culturing liquid according to claim 1 is characterized in that, marine alga can be single algae, also can be the compound algae of two or more algae.
3. micro algae culturing liquid according to claim 1 is characterized in that, marine alga can be a sea-tangle, also can be laver, red hair algae.
4. micro algae culturing liquid according to claim 1 is characterized in that, also includes extract of soybean in the component.
5. micro algae culturing liquid according to claim 1 is characterized in that, can also include Ginger P.E in the component, or aloe extract, or garlic P.E.
6. the preparation method of a micro algae culturing liquid is characterized in that, may further comprise the steps,
1) selects and cleans marine alga;
2) marine alga is dropped into after the leaching liquor broken;
3) algae slurry is filtered, removes photoresist clear liquid;
4) concentrate clear liquid;
5) culture fluid will promptly be got after the concentrate high temperature high pressure process.
7. the preparation method of micro algae culturing liquid according to claim 6 is characterized in that, can also be following steps,
1) selects and cleans first marine alga and second marine alga;
2) with broken after first marine alga input leaching liquor, the algae of winning slurry;
3) with broken after second marine alga input leaching liquor, get second algae slurry;
4) mix first algae slurry and second algae slurry, get the mixed algae slurry;
5) mixed algae slurry is filtered, removes photoresist clear liquid;
6) concentrate clear liquid;
7) culture fluid will promptly be got after the concentrate high temperature high pressure process.
8. the preparation method of micro algae culturing liquid according to claim 7 is characterized in that, first algae slurry is a sea-tangle, and second algae slurry is a laver.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011341475A CN1278601C (en) | 2001-11-03 | 2001-11-03 | Micro algae culturing liquid and its prepn process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011341475A CN1278601C (en) | 2001-11-03 | 2001-11-03 | Micro algae culturing liquid and its prepn process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1416691A CN1416691A (en) | 2003-05-14 |
| CN1278601C true CN1278601C (en) | 2006-10-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB011341475A Expired - Fee Related CN1278601C (en) | 2001-11-03 | 2001-11-03 | Micro algae culturing liquid and its prepn process |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416992B (en) * | 2016-09-28 | 2019-05-28 | 国家海洋局北海环境监测中心 | A kind of Enteromorpha special nutrient fluid and preparation method thereof |
| CN113046246A (en) * | 2019-12-26 | 2021-06-29 | 云南爱尔发生物技术股份有限公司 | Culture solution preparation method based on microalgae culture |
| CN113046247A (en) * | 2019-12-26 | 2021-06-29 | 云南爱尔发生物技术股份有限公司 | Microalgae culture method adopting continuous feeding mode |
| CN111548971A (en) * | 2020-06-02 | 2020-08-18 | 江苏海洋大学 | Preparation method of economic microalgae high-efficiency culture medium based on laver processing wastewater |
| CN112608850A (en) * | 2020-12-18 | 2021-04-06 | 天津现代晨辉科技集团有限公司 | Preparation method of microalgae culture solution |
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