CN1275315A - Large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball - Google Patents
Large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball Download PDFInfo
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- CN1275315A CN1275315A CN 99107899 CN99107899A CN1275315A CN 1275315 A CN1275315 A CN 1275315A CN 99107899 CN99107899 CN 99107899 CN 99107899 A CN99107899 A CN 99107899A CN 1275315 A CN1275315 A CN 1275315A
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Abstract
A quick propagation method for industrialized large-scale production of miniature seed ball of colour common callalily includes the following steps: firstly, making quick propagation of test tube seedling of colour common callalily, then inducing seed ball so as to make it overstep the transplanting link, so that said invention can quickly propagate the miniature seed ball of colour common callalily, and said seed ball is easy to store and transport, and its cost is low.
Description
The present invention relates to the propagation method of miniature kind of ball of calla lily, particularly adopt the method for quickly breeding of batch production large-scale production.
In recent years, test tube balling-up technical research more and more comes into one's own, but has only potato test tube balling-up technology to use more successful in large-scale production.At present existing 8 units of China are engaged in the research work of potato seed ball.States such as the U.S., the Soviet Union, Holland, Belgium, France all utilize this balling-up technology to produce a large amount of high-quality test tube kind balls, form the micro potato industry, have obtained high economic benefit.The patent relevant with micro potato just has more or less a hundred both at home and abroad, but the flowering bulb aspect, the patent that relevant kind ball is induced is quite few.And the patent of common calla test tube balling-up aspect yet there are no report.
Calla lily is the new cutting flower variety that just rose in recent years, and in states such as New Zealand, Holland, the common calla industry is very flourishing.Traditional common calla propagation method is that branch is planted bottom set, and this method reproduction speed is quite slow, and the main mode of common calla breeding at present is the tissue-culturing rapid propagation test-tube plantlet, but this method expense ground, take a lot of work, time-consuming, remote transportation is very inconvenience also.
The objective of the invention is: a kind of method is provided, can carries out the production of miniature kind of ball of batch production calla lily by extensive fast and low-cost.
The present invention is achieved in that at first that calla lily is carried out test-tube plantlet is numerous soon, then test-tube plantlet is carried out miniature kind of ball and induces.
Test-tube plantlet numerous process of determining comprises:
A. the calla lily explant is gathered: select robust growth not have the plant of damage by disease and insect, get its kind ball, scrape and depollute or damage location, be cut into 0.1-1.5cm
2The size sprout tuber, flowing water flushing then.
B. explant sterilization, inoculation: the secondary sterilization method is taked in sterilization.For the first time use 75% alcohol successively, 0.1% bromogeramine, aseptic water washing is used in the sterilization of 0.1% mercuric chloride at last; For the second time sterilisation step is constant, but the time shorten slightly, with inoculating behind the aseptic water washing, medium is MS+BA0.1-10.0ppm+NAA0.01-5.0ppm at last.
C. the grow thickly generation and the test-tube plantlet subculture of bud: after 2-4 month, produce the bud of growing thickly in inoculation on the MS medium.The bud of will growing thickly divides simple bud switching, the bud clump can every month subculture 1 time.
The miniature kind of ball process of inducing comprises:
A. test tube is induced balling-up: select more than the high 2cm, the raw material that the above healthy and strong test-tube plantlet of the thick 0.2cm of stem is produced as miniature kind of ball, the test-tube plantlet access is contained in the liquid nutrient medium of MS-BA0.1-10.0ppm+ paclobutrazol 0.0-10.0ppm, leave standstill and cultivate the 2-3 month, can form miniature kind of ball of 5-15mm diameter.
B. envirment factor is coerced balling-up: with the above test-tube plantlet of the thick 0.2cm of stem, direct transplanting is delayed seedling 20-30 days in nutritive cube.According to selecting high temperature induction or low temperature induction residing season.Handling the 2-3 month under the 5-10 ℃ of cryogenic conditions or under 26-32 ℃ of hot conditions.The acrial part of seedling all or part of withered after, the kind ball in the nutritive cube can be dug out and preserve.
C. hormone is coerced balling-up: with the above test-tube plantlet of the thick 0.2cm of stem, direct transplanting is delayed seedling 20 days-30 days in nutritive cube.Under 25 ± 2 ℃ of environmental conditions, ABA0.5-10.0ppm is foliage-spray 2 times weekly, and it is all or part of withered until the seedling acrial part to continue the 2-3 month, the kind ball in the native alms bowl can be dug out and preserve.
In the fast numerous process of above-mentioned test-tube plantlet, explant best sample time is April or December, and the medium optimum formula is: MS+BA0.5ppm+NAA0.2ppm, required envirment factor is: 25 ± 2 ℃ of temperature, illumination every day 8-14 hour, 12 hours the bests wherein, intensity of illumination is more than the 4000LX.
Above-mentioned miniature kind of ball induced in the process, and it is MS+BA0.5ppm+ paclobutrazol 6.0ppm that test tube is induced the required culture medium prescription of balling-up, PH5.8.Required envirment factor is: 28 ± 2 ℃ of temperature, and suitably dark the cultivation, intensity of illumination 100-600LX, the best is 200-400LX.
Above-mentioned miniature kind of ball induced in the process, and the temperature of coercing that the environment-stress balling-up is required is: the required optimum temperature of low temperature stress is 5 ℃; The required optimum temperature of high temperature stress is 29 ℃.
Above-mentioned miniature kind of ball induced in the process, and hormone is coerced the required optimum condition of balling-up and is: ABA2.0ppm foliage-spray, 2 times weekly.
It is numerous soon that the present invention at first carries out test-tube plantlet to calla lily, carries out kind of a ball then and induce, thereby gone beyond the transplanting link, both saved the soil, saves again and transplant and the field management of balling-up, thereby reduce cost; The 2-3 month in balling-up cycle of the present invention, if carrying out the anniversary produces, can carry out 4-6 time every year, every bottle is once changeed the 8-12 young plant, calculates by 90% balling rate, then can produce 40-60 ball per year for every bottle, five layers of culturing rack that take up an area of about 0.5 square meter can be produced ten thousand of kind of ball 2-3 per year, so reproduction speed of the present invention is fast, high efficiency can be carried out large-scale industrialized production; Of the present invention kind of ball is convenient to storage and transportation, reduces the selling cost in the sales process, and the kind ball is transplanted simple, is convenient to popularizing planting; It is to carry out in the liquid medium within that the present invention plants that ball induces, liquid culture does not need agar, so saved spending, liquid culture is also Zao than solid culture balling, it is many that same area holds the plant number, liquid nutrient medium also have preparation easily, add characteristics such as nutrient solution is convenient; The present invention just can carry out under simple and easy conditions of tissue culture, and is simple to operate, only needs the general technology workman just can operate.
The invention will be further described below in conjunction with embodiment:
The first step: the foundation of calla lily test-tube plantlet tissue-culturing rapid propagation system:
At the beginning of 4 months, on healthy seedling, gather the kind ball of band bud, scrape with blade and depollute or damage location, be cut into the band sprout tuber of 0.1-1.5cm size, stem apex stays 0.2-0.5cm, flowing water flushing 3 hours.The secondary sterilization method is taked in sterilization: for the first time with 75% alcohol 3 minutes, and 0.1% bromogeramine 7 minutes, 0.1% mercuric chloride 3 minutes, aseptic water washing 3 times.Disinfectant is constant for the second time, but time length has adjustment slightly.At last with inoculation behind the aseptic water washing 5 times.
Explant after the sterilization is seeded on the MS medium, and nutrient media components is:
1)MS+BA?0.1ppm+NAA0.05ppm，
2)MS+BA?0.5ppm+NAA0.2ppm，
3)MS+BA?2.0ppm+NAA1.0ppm，
In these three kinds of medium, agar 6.5g/l, sucrose 3%, PH5.8.Every 100ml triangular flask medium of falling 40ml, 5 explants of every bottle graft kind, the external environment factor is 25 ± 2 ℃, 12 hours every days of illumination (6000LX).The result: the bud of growing thickly occurs after No. 1 and No. 3 medium explants inoculation June, No. 2 medium the bud of growing thickly occurs after April.(No. 2 the most suitable calla lily explant differentiation of medium are described).The budlet that will grow thickly is divided the simple bud switching, and subculture was 1 time in every month.Must there be substantial light to shine (4000LX) during this to avoid test-tube plantlet excessive growth.In addition, add the 2.0ppm paclobutrazol in medium, strong sprout, effect was very good, also can make 1 times of the thick growth of test-tube plantlet under the low light level.
Second step: the inducing of miniature kind of ball:
1. test tube is induced balling-up:
Select more than the high 2cm, the above healthy and strong test-tube plantlet of the thick 0.2cm of stem is seeded in the liquid nutrient medium, and every bottle of 8-12 young plant guarantees that every strain test-tube plantlet all stands upright in the liquid nutrient medium.Nutrient media components is: MS+BA0.5ppm+ paclobutrazol 6.0ppm, sucrose are 6%, PH5.8.But all balling-up of medium liquid and solid, but the liquid nutrient medium cost is low, and balling ratio and balling-up size all obviously are better than solid culture medium.Postvaccinal triangular flask is moved into 28 ± 2 ℃ of temperature, under the suitably dark condition of culture, i.e. intensity of illumination 400LX, leave standstill cultivate 2 week the back just can observe the test-tube plantlet stem foot phenomenon of expanding is arranged.2-3 just can form the miniature bead of 5-15mm diameter after the month, balling ratio reaches more than 90%.Stem top is then slowly withered because nutrient refluxes.
Above-mentioned other conditions are constant, and BA concentration changes: when BA is 2.0ppm, and balling ratio 86.5%; BA is 3.0 o'clock, and balling ratio is 90.6%ppm; When BA was 4.0ppm, balling ratio was 88.3%; When BA was 5.0ppm, balling ratio was 86.5%.
Suppose that every blake bottle puts 10 seedlings, by 90% survival rate, one-period can be produced 9 balls, one five layers culturing rack (every layer of 125 * 45cm
2, can put 133 bottles) and can put 665 bottles, then 1 cycle of culturing rack can be produced about 6000 balls, produce ten thousand balls of 2-3 per year, and a culturing rack only takes up an area of 1.25 * 0.45=0.56cm
2
2. environment-stress balling-up:
Select high 2cm, the above healthy and strong test-tube plantlet of the thick 0.2cm of stem is disinfected the back direct transplanting in the nutritive cube of bore 10cm, and slow seedling is 20 days under 25 ± 2 ℃ of conditions.Seedling-slowing stage notes keeping humidity.According to selecting high temperature induction or low temperature induction residing season.Handling 2-3 month under the 5-10 ℃ of cryogenic conditions or under 28-32 ℃ of hot conditions.After the acrial part of seedling is withered fully, the kind ball in the nutritive cube is dug out preserve.
3. hormone is coerced balling-up:
Select more than the height of seedling 2cm, the above healthy and strong test-tube plantlet of the thick 0.2cm of stem is disinfected the back direct transplanting in the nutritive cube of bore 10cm, slow seedling 20 days.Under 25 ± 2 ℃ of environmental conditions, ABA2.0ppm is foliage-spray 2 times weekly, continues 2-3 month to seedling acrial part nutrient total reflux, the kind ball in the nutritive cube is dug out preserve.
The bead storage: generally preserve with refrigerator, miniature kind of ball after being about to gather in the crops dries at ambient temperature, is loaded on then in the cloth bag, stores under 4 ℃ of conditions, can plant after January.
Claims (9)
1. large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball, it is numerous soon to it is characterized in that at first calla lily is carried out test-tube plantlet, then test-tube plantlet is carried out miniature kind of ball and induces.
2. large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball according to claim 1 is characterized in that the fast numerous process of described test-tube plantlet comprises:
A. the calla lily explant is gathered: select robust growth not have the plant of damage by disease and insect, get its kind ball, scrape and depollute or damage location, be cut into 0.1-1.5cm
2The size sprout tuber, flowing water flushing then.
B. explant sterilization, inoculation: the secondary sterilization method is taked in sterilization.For the first time use 75% alcohol successively, 0.1% bromogeramine, aseptic water washing is used in the sterilization of 0.1% mercuric chloride at last; For the second time sterilisation step is constant, but the time shorten slightly, with inoculating behind the aseptic water washing, medium is MS+BA0.1-10.0ppm+NAA0.01-5.0ppm at last.
C. the grow thickly generation and the test-tube plantlet subculture of bud: after 2-4 month, produce the bud of growing thickly in inoculation on the MS medium.The bud of will growing thickly divides simple bud switching, the bud clump can every month subculture 1 time.
3. large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball according to claim 1, it is characterized in that the described miniature kind of ball process of inducing is that test tube is induced the balling-up process: select more than the high 2cm, the raw material that the above healthy and strong test-tube plantlet of the thick 0.2cm of stem is produced as miniature kind of ball, the test-tube plantlet access is contained in the liquid nutrient medium of MS-BA0.1-10.0ppm+ paclobutrazol 0.0-10.0ppm, leave standstill and cultivate the 2-3 month, can form miniature kind of ball of 5-15mm diameter.
4. large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball according to claim 1, it is characterized in that the described miniature kind of ball process of inducing is that envirment factor is coerced the balling-up process: with the above test-tube plantlet of the thick 0.2cm of stem, direct transplanting is delayed seedling 20-30 days in nutritive cube.According to selecting high temperature induction or low temperature induction residing season.Handling the 2-3 month under the 5-10 ℃ of cryogenic conditions or under 26-32 ℃ of hot conditions.The acrial part of seedling all or part of withered after, the kind ball in the nutritive cube can be dug out and preserve.
5. large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball according to claim 1, it is characterized in that the described miniature kind of ball process of inducing is that hormone is coerced the balling-up process: with the above test-tube plantlet of the thick 0.2cm of stem, direct transplanting is delayed seedling 20 days-30 days in nutritive cube.Under 25 ± 2 ℃ of environmental conditions, ABA0.5-10ppm is foliage-spray 2 times weekly, and it is withered wholly or in part until the seedling acrial part to continue the 2-3 month, the kind ball in the nutritive cube can be dug out and preserve.
6. large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball according to claim 1 and 2, it is characterized in that in the fast numerous process of described test-tube plantlet, explant sampling Best Times is April or December, the medium optimum formula is Ms+BA0.5ppm+NAA0.2ppm, required envirment factor is: 25 ± 2 ℃ of temperature, illumination every day 8-14 hour, 12 hours the bests wherein, intensity of illumination is more than the 4000LX.
7. according to claim 1 or 3 described large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball, it is characterized in that described miniature kind of ball induce in the process, it is Ms+BA0.5ppm+ paclobutrazol 6.0ppm that test tube is induced the required medium optimum formula of balling-up, PH5.8.Required envirment factor is: 28 ± 2 ℃ of temperature, and suitably dark the cultivation, intensity of illumination is 100-600LX, the best is 200-400LX.
8. according to claim 1 or 4 described large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball, it is characterized in that described miniature kind of ball induce in the process, the temperature of coercing that the environment-stress balling-up is required is: the required optimum temperature of low temperature stress is 5 ℃; The required optimum temperature of high temperature stress is 29 ℃.
9. large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball according to claim 1 and 2 is characterized in that described miniature kind of ball induce in the process, and hormone is coerced the required optimum condition of balling-up and is: ABA2.0ppm foliage-spray, 2 times weekly.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB991078993A CN1139316C (en) | 1999-06-01 | 1999-06-01 | Large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball |
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|---|---|---|---|
| CNB991078993A CN1139316C (en) | 1999-06-01 | 1999-06-01 | Large scale quick breeding method for color Zantedeschia aethiopica miniature seed ball |
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| CN1275315A true CN1275315A (en) | 2000-12-06 |
| CN1139316C CN1139316C (en) | 2004-02-25 |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331383C (en) * | 2004-02-04 | 2007-08-15 | 贵州科学院 | Calla high yield cultivation method |
| CN100386016C (en) * | 2005-12-30 | 2008-05-07 | 云南省农业科学院 | Off-season cultivation method for cutting flower of colorful common callality |
| CN100574614C (en) * | 2007-12-10 | 2009-12-30 | 浙江省农业科学院 | A kind of calla lily germplasm resources in vitro conservation method |
| CN101228826B (en) * | 2007-12-10 | 2010-06-02 | 浙江省农业科学院 | Method of relieving seedball dormancy and promoting efflorescence for zantedeschia hybrida |
| CN102067818A (en) * | 2010-11-19 | 2011-05-25 | 武汉市蔬菜科学研究所 | Inducing technology of test tube lotus root |
| CN102067783A (en) * | 2010-11-19 | 2011-05-25 | 武汉市蔬菜科学研究所 | Miniature lotus root seed breeding technique for lotus root |
| CN101627729B (en) * | 2008-07-17 | 2011-11-23 | 北京市农林科学院 | High-quality corm of colorful calla and method for cultivating same |
| CN102379239A (en) * | 2011-09-09 | 2012-03-21 | 云南省农业科学院花卉研究所 | Method of breeding novel cultivar of zantedeschia hybrida by artificial supplementary pollination |
| CN102428825A (en) * | 2011-10-25 | 2012-05-02 | 云南省农业科学院花卉研究所 | Breeding method of Anemone coronaria balls |
| CN109526749A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of common calla stem tuber method for tissue culture |
| CN110972951A (en) * | 2019-12-24 | 2020-04-10 | 云南农业大学 | Method for rejuvenating and breaking dormancy of calla tissue culture seedlings |
| CN112042532A (en) * | 2019-10-18 | 2020-12-08 | 江苏省中国科学院植物研究所 | Culture medium for inducing calluses to generate and application of culture medium in establishment of calluses regeneration system |
-
1999
- 1999-06-01 CN CNB991078993A patent/CN1139316C/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1331383C (en) * | 2004-02-04 | 2007-08-15 | 贵州科学院 | Calla high yield cultivation method |
| CN100386016C (en) * | 2005-12-30 | 2008-05-07 | 云南省农业科学院 | Off-season cultivation method for cutting flower of colorful common callality |
| CN100574614C (en) * | 2007-12-10 | 2009-12-30 | 浙江省农业科学院 | A kind of calla lily germplasm resources in vitro conservation method |
| CN101228826B (en) * | 2007-12-10 | 2010-06-02 | 浙江省农业科学院 | Method of relieving seedball dormancy and promoting efflorescence for zantedeschia hybrida |
| CN101627729B (en) * | 2008-07-17 | 2011-11-23 | 北京市农林科学院 | High-quality corm of colorful calla and method for cultivating same |
| CN102067783A (en) * | 2010-11-19 | 2011-05-25 | 武汉市蔬菜科学研究所 | Miniature lotus root seed breeding technique for lotus root |
| CN102067818A (en) * | 2010-11-19 | 2011-05-25 | 武汉市蔬菜科学研究所 | Inducing technology of test tube lotus root |
| CN102067783B (en) * | 2010-11-19 | 2012-06-20 | 武汉市蔬菜科学研究所 | Miniature lotus root seed breeding technique for lotus root |
| CN102379239A (en) * | 2011-09-09 | 2012-03-21 | 云南省农业科学院花卉研究所 | Method of breeding novel cultivar of zantedeschia hybrida by artificial supplementary pollination |
| CN102428825A (en) * | 2011-10-25 | 2012-05-02 | 云南省农业科学院花卉研究所 | Breeding method of Anemone coronaria balls |
| CN102428825B (en) * | 2011-10-25 | 2013-10-16 | 云南省农业科学院花卉研究所 | Breeding method of Anemone coronaria balls |
| CN109526749A (en) * | 2018-12-29 | 2019-03-29 | 贵州大学 | A kind of common calla stem tuber method for tissue culture |
| CN112042532A (en) * | 2019-10-18 | 2020-12-08 | 江苏省中国科学院植物研究所 | Culture medium for inducing calluses to generate and application of culture medium in establishment of calluses regeneration system |
| CN112042532B (en) * | 2019-10-18 | 2022-01-11 | 江苏省中国科学院植物研究所 | Culture medium for inducing calluses to generate and application of culture medium in establishment of calluses regeneration system |
| CN110972951A (en) * | 2019-12-24 | 2020-04-10 | 云南农业大学 | Method for rejuvenating and breaking dormancy of calla tissue culture seedlings |
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|---|---|
| CN1139316C (en) | 2004-02-25 |
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