CN102067783A - Miniature lotus root seed breeding technique for lotus root - Google Patents
Miniature lotus root seed breeding technique for lotus root Download PDFInfo
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Abstract
The invention discloses a miniature lotus root seed breeding technique, and belongs to the technical field of vegetable seedling production. The method comprises the following steps that: tissue culture seedlings of lotus root, test-tube lotus root, terminal buds and axillary buds of lotus root, lotus seeds and the like are cultured in containers with the diameter of 15 to 50cm and the height of 8 to 60cm, and grow and develop into miniature lotus root seeds. The miniature lotus root seed breeding technique comprises the following steps of: preparing soil, arranging containers, and filling soil (mud or other substrates) until the soil is 2/3 of volumes of the containers; after the mean daily temperature is stably higher than 13 DEG C, culturing lotus root brood bodies in the prepared containers; adding water or nutrient solution into the containers; performing management such as water depth regulation, top application, weed removal, pest control and the like in the growth and development process of the miniature lotus root seeds; and breeding to obtain the miniature lotus root seeds with at least 3-5 knots, the length of 12 to 80cm and the mass of 20 to 1,500g. The technique can make the lotus root brood bodies miniaturized.
Description
Technical field
The present invention relates to a kind of miniature kind of lotus root propagation technique of lotus rhizome, belong to the vegetable seedling production technical field.
Technical background
Lotus rhizome is a kind of important aquatic economic crops, and both tool ate, views and admires and medical value, and the gardening of also having beautifies with environmental ecology such as purifying water body and is worth.Simultaneously, lotus rhizome is one of most characteristic in China aquatic vegetable crop, that area under cultivation is maximum aquatic vegetable crop, about 5,000,000 mu of the annual area under cultivation of lotus rhizome, therefore being in great demand to the lotus root kind.In recent years, along with the adjustment of agricultural structure, the economic worth of lotus rhizome and social value seem more and more outstanding, and a lot of paddy fields, fish pond etc. also are used to plant lotus rhizome by the peasant.And in the old producing region of lotus rhizome, owing to plant same kind every year, do not upgrade, make that lotus root varieties is aging, degradation phenomena is serious, cause output to reduce, it is bad that quality (comprising edible quality and marketing quality) becomes.At this critical conditions, the lotus rhizome new varieties are all actively being changed in all parts of the country.But conventional lotus root varieties is based on vegetative propagation, and it is low to show reproduction coefficient, and easily in spite of illness, volume is big, is unfavorable for the problem such as long-distance transport, popularization of seedling, has limited the popularization of lotus rhizome new varieties.Replace the lotus rhizome tradition with kind of a mode with miniature kind of lotus root,, satisfy the production demand greatly accelerating the speed of applying of national lotus rhizome new varieties.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of miniature kind of lotus root propagation technique of lotus rhizome, breeds the miniature seedling of high-quality lotus rhizome fast.It is slow that it has overcome conventional lotus root reproduction speed, and volume is big, be not easy to shortcoming such as transportation, makes the microminiaturization of lotus rhizome seedling, when being convenient to transport, also is convenient to the production of peasant's field planting.
The technical solution adopted for the present invention to solve the technical problems is: a kind of miniature kind of lotus root propagation technique, comprise: get the terminal bud of tissue cultivating seedling, test tube lotus root, lotus rhizome of lotus rhizome and axillalry bud, lotus seed etc. as brood body, cultivate in diameter 15～50cm, in the container of high 8～60cm, through planting on mud or other matrix, grow, breeding forms miniature kind of lotus root.Concrete steps are:
The 1st step: prepare container and matrix.The cleaning weeds in field, container is put in the leveling field, container diameter 15～50cm, high 8～60cm bankets, mud or other matrix is to 2/3 of container height.
The 2nd step: field planting.After daily mean temperature is stable at more than 13 ℃, brood bodies such as the terminal bud of the tissue cultivating seedling of lotus rhizome, test tube lotus root, lotus rhizome and axillalry bud, lotus seed are cultivated in the off-the-shelf container that is added with mud or other matrix.
The 3rd step: add entry or nutrient solution.In container, add entry or nutrient solution, make the plant normal growth.
The 4th step: precision management.In miniature kind of lotus root growth and development process, carry out management such as the depth of water is regulated, topdresses, weeded a garden, the extermination of disease and insect pest.
The 5th step: breeding forms miniature kind of lotus root.Breeding forms has minimum 3～8 joints, long 12～80cm, the miniature kind of lotus root of quality 20g～1500g.
Optimized technical scheme of the present invention is to adopt the brood body of test tube lotus root as lotus rhizome, and the cultivation of wherein said test tube lotus root comprises the steps:
(1), stem apex starts: terminal bud or the lateral bud of choosing anosis lotus rhizome are explant, peel off outer leaf sheath, cutting stem apex is inoculated in the lotus rhizome stem apex and starts on the medium and cultivate, until growing into simple bud or clump bud, wherein stem apex startup medium is 1/2MS+6-BA0.5～4.0mg/L+NAA0.1～0.5mg/L+3.0% sucrose+agar 0.5～0.6%;
(2), shoot proliferation: simple bud or clump bud be transferred to carry out shoot proliferation on the subculture medium and cultivate, until producing new clump bud, wherein subculture medium is 1/2MS+6-BA0.5～2.0mg/L+NAA0.1～1.0mg/L+3.0% sucrose+agar 0.5～0.6%;
(3), take root: the clump bud is divided into simple bud, be transferred to root induction on the root media, until the formation test-tube plantlet of taking root, wherein root media is 1/2MS+6-BA0.5～1.0mg/L+IBA0.1～1.5mg/L+AC0.5～1.0g/L+5.0% sucrose+agar 0.5～0.6%;
(4), induce the test tube lotus root: behind the culture of rootage 30d, liquid nutrient medium 1/2MS+6-BA0.1～1.0mg/L+NAA0.1～0.5mg/L+3.0～12.0% sucrose is poured in the culture vessel, induced to form the test tube lotus root.
The pH value of each stage medium of described test tube lotus root is 5.8～6.0, and cultivation temperature is 24～26 ℃, and intensity of illumination is 2000～2500lx, and every day, light application time was 10h.
Medium full name of the present invention is:
MS:Murashige﹠amp; Skoog (1962) medium; The 6-BA:6-benayl aminopurine;
NAA: methyl; IBA: indolebutyric acid; AC: active carbon.
The present invention compared with prior art has following advantage:
1, the present invention has proposed a kind of miniature kind of lotus root propagation technique first, do kind to compare with the root-like stock (conventional lotus root) that expands with maturation of present employing and have significant advantage and economic results in society: every mu of sowing quantity of general conventional lotus root is 200～300kg, the conventional lotus root kind that 1 mu of ground is produced only can be planted for 8～10 mu of ground, proportion of goods damageds height in the long-distance transport process particularly, the freight height.The present invention utilizes the terminal bud of tissue cultivating seedling, test tube lotus root, lotus rhizome of lotus rhizome and axillalry bud, lotus seed etc. as brood body, and breeding forms miniature kind of lotus root, has the reproduction coefficient height, and volume is little, is convenient to advantages such as transportation.And every mu of sowing quantity of miniature kind of lotus root only is 20～40kg (120～150), every 667m
2The miniature kind of lotus root of producing can supply 20～40 mu of field plantings, and the proportion of goods damageds are little in the transportation of way far away, and freight is low.
2, miniature kind of lotus root has the advantage of test tube lotus root and conventional lotus root concurrently, overcome the deficiency of the two, has the cost of saving, saving of labor, the conventional incomparable advantage of lotus root of planting such as efficient; Simultaneously, culture technique is simple and easy to do, is beneficial to the peasant and grasps, and replaces the lotus rhizome tradition with kind of a mode with miniature kind of lotus root, will accelerate the speed of applying of national lotus rhizome new varieties greatly.
3, miniature kind of lotus root propagation technique of lotus rhizome provided by the present invention is applicable to all kinds lotus rhizome (lotus root lotus, Zi Lian and Hua Lian).
Embodiment
Embodiment 1
Get No. five test tube lotus roots of E Lian and cultivate in the bulge of diameter 30cm, high 12cm, through growing, breeding forms miniature kind of lotus root.Concrete steps are:
The 1st step: put container, filling substrate: the cleaning weeds in field, container is put in the leveling field, and (mud) bankets in container.Container diameter 30cm, high 12cm, banket (mud) is to 2/3 of container depth.
The 2nd step: field planting: daily mean temperature is stable at more than 13 ℃, with the test tube lotus root of lotus rhizome Hubei Province lotus No. five, transplants in off-the-shelf container.
The 3rd step: add entry or nutrient solution: in container, add 1cm water, make the plant normal growth.
The 4th step: precision management: in miniature kind of lotus root growth and development process, carry out management such as the depth of water is regulated, topdresses, weeded a garden, the extermination of disease and insect pest.Before upright leaf grew, the depth of water was flooded the suitable 3cm of vessel surface; After upright leaf grew, water level can be deepened gradually, but should not surpass 10cm; The suitable 10cm of the overwintering period depth of water.Should be when 2～3 upright leaves every 667m
2Sealing fertilizer 50kg; During 5～6 upright leaves, every 667m
2Execute urea 15kg and potassium sulphate 10kg.Insect pests such as attention control aphid, twill leaf moth.
The 5th step: breeding forms has minimum 3～5 joints, long 12～80cm, the miniature kind of lotus root of quality 50g～1500g.
Embodiment 2
Get the terminal bud of No. five lotus rhizomes of E Lian, cultivating in diameter is in the container of circle of 35cm, high 12cm, and through growing, breeding forms miniature kind of lotus root.Concrete steps are:
The 1st step: put container, filling substrate: the cleaning weeds in field, container is put in the leveling field, and (mud) bankets in container.Container diameter 30cm, high 12cm, banket (mud) is to 2/3 of container depth.
The 2nd step: field planting: daily mean temperature is stable at more than 13 ℃, with the terminal bud of No. five miniature kind of lotus roots of Hubei Province lotus, cultivates in off-the-shelf container.
The 3rd step: add entry or nutrient solution: in container, add 2cm deep water, make the plant normal growth.
The 4th step: precision management: in miniature kind of lotus root growth and development process, carry out management such as the depth of water is regulated, topdresses, weeded a garden, the extermination of disease and insect pest.
The 5th step: breeding forms has minimum 3～5 joints, long 12～80cm, the miniature kind of lotus root of quality 50g～1500g.
The propagation method of test tube lotus root of the present invention is as follows:
Embodiment 3
1. stem apex starts: get the terminal bud of No. five kinds of anosis Hubei Province lotus that clean up, through 75% after alcohol-pickled 30 seconds, use 0.1%HgCl
2Solution soaking 3～12 minutes is used aseptic water washing 3 times again, each 3～5 minutes.After finishing above-mentioned surface sterilization handling procedure, peel off outer leaf sheath with scalpel, cut the long stem apex of 0.3～0.6cm, be inoculated on the lotus rhizome stem apex differential medium 1/2MS+6-BA2.0mg/L+NAA0.5mg/L+3.0% sugar, in temperature is 24～26 ℃, under intensity of illumination 2000～2500lx, illumination every day 10h produces the bud or the bud of growing thickly behind 30～45d.The pH value of lotus rhizome stem apex differential medium is 5.8～6.0.
2. shoot proliferation: bud or clump bud are transferred to subculture medium 1/2MS+6-BA1.0mg/L+NAA0.5mg/L+3.0% sugar shoot proliferation, in temperature is 24～26 ℃, under intensity of illumination 2000～2500lx, illumination every day 10h, 30～40d produce new bud or clump bud.Subculture medium pH value is 5.8～6.0.
3. take root: the clump bud is divided into simple bud, being transferred to the root induction of root media 1/2MS+6-BA0.5mg/L+IBA1.0mg/L+AC0.5g/L+5.0% sugar, is 24～26 ℃ in temperature, under illuminance 2000～2500lx, illumination every day 10h, the 25d formation test-tube plantlet of taking root.Root media pH value is 5.8～6.0.
4. induce the test tube lotus root: behind the culture of rootage 30d, (1/2MS+6-BA0.1～1.0mg/L+NAA0.1～0.5mg/L+3.0～12.0% sucrose) pours in the culture vessel with liquid nutrient medium, under being 24～26 ℃, intensity of illumination 2000～2500lx, temperature cultivates, illumination every day 10h induces to form the test tube lotus root.30d induces later on and forms the test tube lotus root.Inducing test tube lotus root medium pH value is 5.8～6.0.
Embodiment 4
Terminal bud with No. 3, sub-lotus kind space is an explant, through stem apex startup, shoot proliferation, take root, induce and form the test tube lotus root.Comprise the steps:
1. stem apex starts: with space is test material No. 3, gets No. 3 terminal buds of anosis space that clean up, and through 75% after alcohol-pickled 30 seconds, uses 0.1%HgCl
2Solution soaking 3～12 minutes is used aseptic water washing 3 times again, each 3～5 minutes.After finishing above-mentioned surface sterilization handling procedure, peel off outer leaf sheath with scalpel, cut the long stem apex of 0.3～0.6cm, be inoculated on the lotus rhizome stem apex differential medium 1/2MS+6-BA2.0mg/L+NAA0.5mg/L+3.0% sugar, in temperature is 24～26 ℃, under intensity of illumination 2000～2500lx, illumination every day 10h produces the bud or the bud of growing thickly behind 30～45d.The pH value of lotus rhizome stem apex differential medium is 5.8～6.0.
2. shoot proliferation: bud or clump bud are transferred to subculture medium 1/2MS+6-BA1.0mg/L+NAA0.5mg/L+3.0% sugar shoot proliferation, in temperature is 24～26 ℃, under intensity of illumination 2000～2500lx, illumination every day 10h, 30～40d produce new bud or clump bud.Subculture medium pH value is 5.8～6.0.3. take root: the clump bud is divided into simple bud, being transferred to the root induction of root media 1/2MS+6-BA0.5mg/L+IBA1.0mg/L+AC0.5g/L+5.0% sugar, is 24～26 ℃ in temperature, under illuminance 2000～2500lx, illumination every day 10h, the 25d formation test-tube plantlet of taking root.Root media pH value is 5.8～6.0,4. induce the test tube lotus root: behind the culture of rootage 30d, (1/2MS+6-BA0.1～1.0mg/L+NAA0.1～0.5mg/L+3.0～12.0% sucrose) pours in the culture vessel with liquid nutrient medium, under being 24～26 ℃, intensity of illumination 2000～2500lx, temperature cultivates, illumination every day 10h induces to form the test tube lotus root.30d induces later on and forms the test tube lotus root.Inducing test tube lotus root medium pH value is 5.8～6.0.
Claims (4)
1. miniature kind of lotus root propagation technique of a lotus rhizome, it comprises the steps:
(1), with container be placed on the leveling field in, in container, fill out the plantation matrix to 2/3 of container height;
(2), after daily mean temperature is stable at more than 13 ℃, the brood body of lotus rhizome is cultivated in container;
(3), in container, add entry or nutrient solution, make the plant normal growth;
(4), in miniature kind of lotus root growth and development process, carry out that the depth of water is regulated, topdresses, weeded a garden, extermination of disease and insect pest management, breeding forms has minimum 3～8 joints, long 12～80cm, the miniature kind of lotus root of quality 20g～1500g.
2. miniature kind of lotus root propagation technique of a kind of lotus rhizome according to claim 1, the brood body of wherein said lotus rhizome are tissue cultivating seedling, the test tube lotus root of lotus rhizome, terminal bud, axillalry bud or the lotus seed of lotus rhizome.
3. miniature kind of lotus root propagation technique of a kind of lotus rhizome according to claim 2, the cultivation of wherein said test tube lotus root comprises the steps:
(1), stem apex starts: terminal bud or the lateral bud of choosing anosis lotus rhizome are explant, peel off outer leaf sheath, cutting stem apex is inoculated in the lotus rhizome stem apex and starts on the medium and cultivate, until growing into simple bud or clump bud, wherein stem apex startup medium is 1/2MS+6-BA0.5～4.0mg/L+NAA0.1～0.5mg/L+3.0% sucrose+agar 0.5～0.6%;
(2), shoot proliferation: simple bud or clump bud be transferred to carry out shoot proliferation on the subculture medium and cultivate, until producing new clump bud, wherein subculture medium is 1/2MS+6-BA0.5～2.0mg/L+NAA0.1～1.0mg/L+3.0% sucrose+agar 0.5～0.6%;
(3), take root: the clump bud is divided into simple bud, be transferred to root induction on the root media, until the formation test-tube plantlet of taking root, wherein root media is 1/2MS+6-BA0.5～1.0mg/L+IBA0.1～1.5mg/L+AC0.5～1.0g/L+5.0% sucrose+agar 0.5～0.6%;
(4), induce the test tube lotus root: behind the culture of rootage 30d, liquid nutrient medium 1/2MS+6-BA0.1～1.0mg/L+NAA0.1～0.5mg/L+3.0～12.0% sucrose is poured in the culture vessel, induced to form the test tube lotus root.
4. miniature kind of lotus root breeding of a kind of lotus rhizome according to claim 3, the pH value of each stage medium of wherein said test tube lotus root is 5.8～6.0, and cultivation temperature is 24～26 ℃, and intensity of illumination is 2000～2500lx, and every day, light application time was 10h.
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| RU2479984C1 (en) * | 2011-11-25 | 2013-04-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Астраханский государственный университет" | Method of cultivation of hindu lotus in containers |
| CN104542297A (en) * | 2015-01-23 | 2015-04-29 | 杨学 | Cultivation method of nine-grade nymphaea hybrid |
| CN105379623A (en) * | 2015-11-27 | 2016-03-09 | 苏州市农业科学院 | Breeding and cultivating method of nelumbo nucifera by using rhizome of lotus in test tube as material |
| CN105475137A (en) * | 2015-12-16 | 2016-04-13 | 杭州市园林文物局灵隐管理处(杭州花圃) | Method for tissue culture taking lotus reproduction and rooting into account |
| CN106576818A (en) * | 2016-12-12 | 2017-04-26 | 梁华 | Lotus root planting method |
| CN109329024A (en) * | 2018-11-17 | 2019-02-15 | 安徽阜阳金丰生态农业有限公司 | A kind of lotus soilless culture method |
| CN112314437A (en) * | 2020-11-05 | 2021-02-05 | 苏州市农业科学院 | Primary culture method for lotus tissue culture |
| CN112544378A (en) * | 2020-12-01 | 2021-03-26 | 简红忠 | Early propagation and efficiency-increasing method for early-maturing lotus roots |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2479984C1 (en) * | 2011-11-25 | 2013-04-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Астраханский государственный университет" | Method of cultivation of hindu lotus in containers |
| CN104542297A (en) * | 2015-01-23 | 2015-04-29 | 杨学 | Cultivation method of nine-grade nymphaea hybrid |
| CN105379623A (en) * | 2015-11-27 | 2016-03-09 | 苏州市农业科学院 | Breeding and cultivating method of nelumbo nucifera by using rhizome of lotus in test tube as material |
| CN105475137A (en) * | 2015-12-16 | 2016-04-13 | 杭州市园林文物局灵隐管理处(杭州花圃) | Method for tissue culture taking lotus reproduction and rooting into account |
| CN106576818A (en) * | 2016-12-12 | 2017-04-26 | 梁华 | Lotus root planting method |
| CN109329024A (en) * | 2018-11-17 | 2019-02-15 | 安徽阜阳金丰生态农业有限公司 | A kind of lotus soilless culture method |
| CN112314437A (en) * | 2020-11-05 | 2021-02-05 | 苏州市农业科学院 | Primary culture method for lotus tissue culture |
| CN112314437B (en) * | 2020-11-05 | 2022-06-07 | 苏州市农业科学院 | Primary culture method for lotus tissue culture |
| CN112544378A (en) * | 2020-12-01 | 2021-03-26 | 简红忠 | Early propagation and efficiency-increasing method for early-maturing lotus roots |
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