CN104542297A - Cultivation method of nine-grade nymphaea hybrid - Google Patents
Cultivation method of nine-grade nymphaea hybrid Download PDFInfo
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- CN104542297A CN104542297A CN201510033745.2A CN201510033745A CN104542297A CN 104542297 A CN104542297 A CN 104542297A CN 201510033745 A CN201510033745 A CN 201510033745A CN 104542297 A CN104542297 A CN 104542297A
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Abstract
The invention relates to a cultivation method of nine-grade nymphaea hybrid. The cultivation method concretely comprises the following steps: S1, preparing the tuber of the nine-grade nymphaea hybrid, and carrying out sterilization on the tuber; S2, pretreating the tuber of the nine-grade nymphaea hybrid, namely, soaking the tuber of the nine-grade nymphaea hybrid in a formalin solution subjected to 1000-time dilution for 30 minutes, and then soaking in a zineb solution subjected to 200-time dilution for 10 minutes; S3, drying the pretreated tuber of the nine-grade nymphaea hybrid; and S4, culturing in a biochemical incubator at the temperature of 32-33 DEG C. According to the method, the tuber of the nine-grade nymphaea hybrid is firstly taken out from soil and is cleaned up; after that, the tuber of the nine-grade nymphaea hybrid is soaked into the zineb solution subjected to 200-time dilution for 4 hours so as to be sterilized. In the step 1, the nine-grade nymphaea hybrid is sterilized, so that bacteria, impurities and the like on the surface of the nine-grade nymphaea hybrid can be removed, and the possibility that the nine-grade nymphaea hybrid is polluted by the bacteria at the later period can be reduced.
Description
Technical field
The present invention relates to nine grades of rank in the feudal regimes perfume lotus flower cultural technique field, more particularly, particularly a kind of cultivation method of nine grades of rank in the feudal regimes perfume lotus flower.
Background technology
In the prior art, the implantation methods of nine grades of rank in the feudal regimes perfume lotus flower is:
Because lotus seeds shell is hard closely knit, need to carry out artificial cut, one of lotus seeds has little prominent point, there is little concave point other end, gall on coarse concrete floor there being that one end of little concave point or with the broken osculum of vice sub-folder, note not crushing embryo, also do not shell.After cut, under the environment of water temperature 20 DEG C-30 DEG C, soak seed, to soak seed for degree, be then placed in indoor, encase with wet towel, temperature arranges 28 DEG C-30 DEG C:, can germinate in two weeks.Be placed on area without shade after sprouting, add intense light irradiation, can not lack of water.Grow radicula and the tender little lotus leaf of 2 ~ 3 childrens after three weeks, treat that leaf is as money shape, root system 3-5 root bar is formed just can load sharing.
Said method is very easy to cause nine grades of rank in the feudal regimes perfume lotus flower to be just subject to the infection of bacterium etc. in the budding period, and causing to germinate just is soaked the situation of rotting.
Summary of the invention
In view of this, the invention provides a kind of cultivation method of nine grades of rank in the feudal regimes perfume lotus flower, to solve the problem.
For achieving the above object, the invention provides following technical scheme:
A cultivation method for nine grades of rank in the feudal regimes perfume lotus flower, comprises step:
S1, stem tuber sterilizing, produce nine grades of rank in the feudal regimes perfume lotus flower stem tuber, and carry out two-stage sterilization to it;
S2, stem tuber pretreatment, carry out pretreatment to nine grades of rank in the feudal regimes perfume lotus flower stem tuber, is soaked in the formalin solution of 1000 times of dilutions and soaks 30 minutes, then dilutes in 200 times of solution in Dai Senxin and soak 10 minutes;
S3, stem tuber are dried, and are dried by pretreated nine grades of rank in the feudal regimes perfume lotus flower stem tuber;
S4, biochemical case are cultivated, and cultivate in biochemical cultivation case at the temperature of 32-33 degree Celsius.
Preferably, in step sl, adopt Dai Senxin to dilute in 200 times of solution and soak 3-4 hour sterilizing.
Preferably, in step s 2, after solution immersion is carried out to nine grades of rank in the feudal regimes perfume lotus flower, the nine grades of rank in the feudal regimes perfume lotus flower after soaking is dried surface moisture in 30 minutes at the temperature of 37-38 degree Celsius in biochemical cultivation case, and then ultraviolet sterilization is carried out to it.
Preferably, in step s3, biochemical cultivation case temperature is transferred to 35 degree, cultivation is carried out 2 days to nine grades of rank in the feudal regimes perfume lotus flower.
Preferably, in step s 4 which, it is specifically cultivated flow process and is:
Biochemical cultivation case temperature, under 32 degree of environment, is cultivated 2 days in the culture fluid of 6-BA1ML+NAA2ML+IBA2ML;
Biochemical cultivation case temperature is under 32 degree of environment, and nine grades of rank in the feudal regimes perfume lotus flower starts to sprout for 5 days, and the 15-20 centimetre that sprouts for 30 days is high, is taken off by the bud that nine grades of rank in the feudal regimes perfume lotus flower sends to be transplanted in pond to support and plants;
The nine grades of rank in the feudal regimes perfume lotus flower of planting in pond is cultivated and is bloomed for 150 days, and blooming 1-2 days can Mining flower.
In the above-mentioned methods, first the stem tuber of nine grades of rank in the feudal regimes perfume lotus flower is taken out in soil, clean up, then put into Dai Senxin and dilute 200 times of solution immersions sterilizing in 4 hours.In step one, sterilizing is carried out to nine grades of rank in the feudal regimes perfume lotus flower, the bacterium on its surface, impurity etc. have been removed, reduces the possibility of later stage nine grades of rank in the feudal regimes perfume lotus flower germ contamination.
In step 2, pretreatment is carried out to nine grades of rank in the feudal regimes perfume lotus flower stem tuber, be soaked in the formalin solution of 1000 times of dilutions and soaked 30 minutes, then soak 10 minutes in the Dai Senxin solution of 200 times of dilutions, by the oven dry of step 3, nine grades of rank in the feudal regimes perfume lotus flower can be made to carry out double absorption to formalin and zineb, thus improve the antibiotic property of nine grades of rank in the feudal regimes perfume lotus flower further, reduce the probability of nine grades of rank in the feudal regimes perfume lotus flower germ contamination dramatically.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the flow chart of the cultivation method of an embodiment of the present invention nine grades of rank in the feudal regimes perfume lotus flower.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Please refer to Fig. 1, Fig. 1 is the flow chart of the cultivation method of an embodiment of the present invention nine grades of rank in the feudal regimes perfume lotus flower.
The invention provides a kind of cultivation method of nine grades of rank in the feudal regimes perfume lotus flower, it specifically comprises step:
S1, produce nine grades of rank in the feudal regimes perfume lotus flower stem tuber, and sterilizing is carried out to it; S2, pretreatment is carried out to nine grades of rank in the feudal regimes perfume lotus flower stem tuber, be soaked in the formalin solutions of 1000 times of dilutions and soak 30 minutes, then dilute in 200 times of solution in Dai Senxin and soak 10 minutes; S3, pretreated nine grades of rank in the feudal regimes perfume lotus flower stem tuber to be dried; S4, to cultivate at the temperature of 32-33 degree Celsius in biochemical cultivation case.
In the above-mentioned methods, first the stem tuber of nine grades of rank in the feudal regimes perfume lotus flower is taken out in soil, clean up, then put into Dai Senxin and dilute 200 times of solution immersions sterilizing in 4 hours.In step one, sterilizing is carried out to nine grades of rank in the feudal regimes perfume lotus flower, the bacterium on its surface, impurity etc. have been removed, reduces the possibility of later stage nine grades of rank in the feudal regimes perfume lotus flower germ contamination.
In step 2, pretreatment is carried out to nine grades of rank in the feudal regimes perfume lotus flower stem tuber, be soaked in the formalin solution of 1000 times of dilutions and soaked 30 minutes, then dilute in 200 times of solution in zineb and soak 10 minutes, by the oven dry of step 3, nine grades of rank in the feudal regimes perfume lotus flower can be made to carry out two-stage sterilization to formalin and zineb, thus improve the antibiotic property of nine grades of rank in the feudal regimes perfume lotus flower further, reduce the probability of nine grades of rank in the feudal regimes perfume lotus flower germ contamination dramatically.
Particularly, adopt zineb to dilute in 200 times of solution and soak 3-4 hour sterilizing.
Particularly, after solution immersion is carried out to nine grades of rank in the feudal regimes perfume lotus flower, the nine grades of rank in the feudal regimes perfume lotus flower after soaking is dried surface moisture in 30 minutes at the temperature of 37-38 degree Celsius in biochemical cultivation case, and then ultraviolet sterilization is carried out to it.
Particularly, biochemical cultivation case temperature is transferred to 35 degree, cultivation is carried out to nine grades of rank in the feudal regimes perfume lotus flower and within one week, makes it carry out two-stage sterilization to bactericidal agent;
Particularly, the concrete cultivation flow process of the nine grades of rank in the feudal regimes perfume lotus flower after process is: biochemical cultivation case temperature, under 32 degree of environment, is cultivated one week in the culture fluid of 6-BA1ML+ENN2ML+IBA2ML; Biochemical cultivation case temperature is under 32 degree of environment, and nine grades of rank in the feudal regimes perfume lotus flower starts to sprout for 5-7 days, and the 15-20 centimetre that sprouts for 30 days is high, root system 5-10 bar root, leaf 5-8 sheet leaf.The bud that nine grades of rank in the feudal regimes perfume lotus flower sends is taken off be transplanted in pond to support and plant; The nine grades of rank in the feudal regimes perfume lotus flower of planting in pond is cultivated and is bloomed for 150 days, and blooming 1-2 days can Mining defloration.
Use said method to concrete operations flow process prepared by nine grades of rank in the feudal regimes perfume lotus flower is:
Nine grades of rank in the feudal regimes perfume lotus flower stem tuber takes out in soil, cleans up, and puts into zineb and dilutes 200 times of solution immersions sterilizing in 4 hours; In formalin container, 1000 times are soaked 30 minutes.Dai Senxin 200 times soaks 10 minutes; Biochemical cultivation case high temperature 38 degree of oven dry 30 minutes, the moisture on the fast stem of nine grades of rank in the feudal regimes perfume lotus flower is by the temperature in biochemical cultivation case, wind, ultraviolet light sterilizing flash baking; Biochemical cultivation case temperature is transferred to 35 degree, carries out cultivation and within 2 days, can make the fast stem two-stage sterilization of nine grades of rank in the feudal regimes perfume lotus flower; Biochemical cultivation case temperature is transferred in the culture fluid of 32 degree: 6-BA1ML+ENN2ML+IBA2ML and is cultivated one week; Biochemical cultivation case temperature is transferred to 32 degree: 5-7 days and is started to sprout, and the 15-20 centimetre that sprouts for 30 days is high.Root system 5-10 bar root, leaf 5-8 sheet leaf, under bud is taken out, enters in pond to support and plants; Support in pond, within 150 days, bloom.Bloom, Mining defloration in 1-2 days.Implantation time can plant 8-10 at replacing seedling; The flower that Mining is plucked, remove out Bao Ye, wash clean, enter low temperature drying case, drying box temperature 19-50 degree 12 hours and can drying, spend anhydrous point to retain original anthocyan, vegetable protein, ultraviolet sterilization, adopt vacuum cracker box masking foil sealing packaging and edible.
The range of application of nine grades of rank in the feudal regimes perfume lotus flower: nine grades of rank in the feudal regimes perfume lotus flower is made tea, nine grades of rank in the feudal regimes perfume lotus flower cake, nine grades of rank in the feudal regimes perfume lotus flower facial mask, nine grades of rank in the feudal regimes perfume lotus flower noodles, nine grades of rank in the feudal regimes perfume lotus flower cake, nine grades of rank in the feudal regimes perfume lotus flower steamed stuffed bun, steamed bun, nine grades of rank in the feudal regimes perfume lotus flower chafing dish, nine grades of rank in the feudal regimes perfume lotus flower dish, nine grades of rank in the feudal regimes perfume lotus flower fresh flower etc.
Adopt the nine grades of rank in the feudal regimes perfume lotus flower that said method is cultivated, in a kind of embodiment, its concrete steps are: spending of having plucked is clean, closely enter drying box 19-50 degree and dry 12 hours, flower is without moisture, retain original anthocyan, vegetable protein, ultraviolet sterilization, adopt vacuum cracker box masking foil sealing packaging and edible.
Explanation
6-BA1mL+NAA2mL+IBA2.5mL implication be: 6-BA is phytocytomine, NAA be plant establishment element, IBA be plant cell growth element.Above-mentioned three kinds of materials to be added up mixing, just can promote that root system of plant is flourishing, improve survival rate, extend the growth year of seedling, improve output and resistance against diseases, reach: root system of plant is flourishing, survival rate is high, extend the vegetative period of seedling, high yield, resistance against diseases effect, culture fluid in cultivate 2 days.
Use cultivation method provided by the invention, its experiment comparison result is as following table:
Result is see such as following table 1 and table 2
Watch 1 is nine grades of rank in the feudal regimes perfume lotus flower stem tuber (10 blocks, every fast Bud Differentiation 10 strains are standard) root-growing agent process contrast reference table:
Result shows: 6-BA1mL+NAA2mL+IBA2.5mL sprouts at most, well developed root system, survival rate are the highest.
Table 2 is that nine grades of rank in the feudal regimes perfume lotus flower is transplanted and Field planting Experimental comparison reference table.
The seedling flowering stage adopting the formula of 6-BA1mL+NAA2mL+IBA2.5mL embodiment three to germinate is early: the average every day moon flower.
Above-mentioned explanation, enables professional and technical personnel in the field realize or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (5)
1. a cultivation method for nine grades of rank in the feudal regimes perfume lotus flower, is characterized in that, comprising:
Step one, produce nine grades of rank in the feudal regimes perfume lotus flower stem tuber, and sterilizing is carried out to it;
Step 2, pretreatment is carried out to nine grades of rank in the feudal regimes perfume lotus flower stem tuber, be soaked in the formalin solutions of 1000 times of dilutions and soak 30 minutes, then dilute in 200 times of solution in Dai Senxin and soak 10 minutes;
Step 3, pretreated nine grades of rank in the feudal regimes perfume lotus flower stem tuber to be dried;
Step 4, to cultivate at the temperature of 32-33 degree Celsius in biochemical cultivation case.
2. the cultivation method of nine grades of rank in the feudal regimes perfume lotus flower according to claim 1, is characterized in that,
In described step one, adopt Dai Senxin to dilute in 200 times of solution and soak 3-4 hour sterilizing.
3. the cultivation method of nine grades of rank in the feudal regimes perfume lotus flower according to claim 1, is characterized in that,
In described step 2, after solution immersion is carried out to nine grades of rank in the feudal regimes perfume lotus flower, the nine grades of rank in the feudal regimes perfume lotus flower after soaking is dried surface moisture in 30 minutes at the temperature of 37-38 degree Celsius in biochemical cultivation case, and then ultraviolet sterilization is carried out to it.
4. the cultivation method of nine grades of rank in the feudal regimes perfume lotus flower according to claim 1, is characterized in that,
In described step 3, biochemical cultivation case temperature is transferred to 35 degree, to nine grades of rank in the feudal regimes perfume lotus flower carry out its Dai Senxin of cultivation 2 angel dilute in 200 times of solution soak carry out double absorption sterilizing.
5. the cultivation method of nine grades of rank in the feudal regimes perfume lotus flower according to claim 1, is characterized in that,
In described step 4, it is specifically cultivated flow process and is:
Biochemical cultivation case temperature, under 32 degree of environment, is cultivated 2 days in the culture fluid of 6-BA1ML+ENN2ML+IBA2ML;
Biochemical cultivation case temperature is under 32 degree of environment, and nine grades of rank in the feudal regimes lotus flower starts to sprout for 5 days, and it is high that 30 days buds grow to 15-20 centimetre, is taken off by the bud that nine grades of rank in the feudal regimes lotus flower sends to be transplanted in pond to support and plants;
The nine grades of rank in the feudal regimes lotus flower of planting in pond is cultivated and is bloomed for 150 days, blooms and gets final product the third place in imperial examination in 2-3 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104813939A (en) * | 2015-02-05 | 2015-08-05 | 南京农业大学 | Method for constructing lotus regeneration system |
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| CN1631075A (en) * | 2004-11-26 | 2005-06-29 | 扬州大学 | Domestication method for lotus root group cultivating de-poisoning breeding |
| CN102067783A (en) * | 2010-11-19 | 2011-05-25 | 武汉市蔬菜科学研究所 | Miniature lotus root seed breeding technique for lotus root |
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| CN1631075A (en) * | 2004-11-26 | 2005-06-29 | 扬州大学 | Domestication method for lotus root group cultivating de-poisoning breeding |
| CN102067783A (en) * | 2010-11-19 | 2011-05-25 | 武汉市蔬菜科学研究所 | Miniature lotus root seed breeding technique for lotus root |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104813939A (en) * | 2015-02-05 | 2015-08-05 | 南京农业大学 | Method for constructing lotus regeneration system |
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Application publication date: 20150429 |