CN1262668C - 转移核酸通过核被膜的细胞转运系统的用途 - Google Patents

转移核酸通过核被膜的细胞转运系统的用途 Download PDF

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Publication number
CN1262668C
CN1262668C CNB008034478A CN00803447A CN1262668C CN 1262668 C CN1262668 C CN 1262668C CN B008034478 A CNB008034478 A CN B008034478A CN 00803447 A CN00803447 A CN 00803447A CN 1262668 C CN1262668 C CN 1262668C
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dna
nuclear
localization signal
sequence
peptide
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CNB008034478A
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Chinese (zh)
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CN1339068A (zh
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G·希本科顿
R·克里斯汀
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Amaxa AG
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Amaxa AG
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Priority claimed from DE19933939A external-priority patent/DE19933939A1/de
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C07K14/003—Peptide-nucleic acids (PNAs)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Transplantation (AREA)
  • Surgery (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
CNB008034478A 1999-01-08 2000-01-03 转移核酸通过核被膜的细胞转运系统的用途 Expired - Fee Related CN1262668C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19900513 1999-01-08
DE19900513.3 1999-01-08
DE19933939.2 1999-07-20
DE19933939A DE19933939A1 (de) 1999-01-08 1999-07-20 Ausnutzung zelleigener Transportsysteme zum Transfer von Nukleinsäuren durch die Kernhülle

Publications (2)

Publication Number Publication Date
CN1339068A CN1339068A (zh) 2002-03-06
CN1262668C true CN1262668C (zh) 2006-07-05

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Family Applications (1)

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CNB008034478A Expired - Fee Related CN1262668C (zh) 1999-01-08 2000-01-03 转移核酸通过核被膜的细胞转运系统的用途

Country Status (8)

Country Link
US (1) US6521456B1 (enExample)
EP (1) EP1141364A1 (enExample)
JP (2) JP3997056B2 (enExample)
CN (1) CN1262668C (enExample)
AU (1) AU764660B2 (enExample)
CA (1) CA2358040C (enExample)
IL (1) IL143983A0 (enExample)
WO (1) WO2000040742A1 (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030069173A1 (en) * 1998-03-16 2003-04-10 Life Technologies, Inc. Peptide-enhanced transfections
AU764660B2 (en) * 1999-01-08 2003-08-28 Lonza Cologne Ag Use of the cell's own transport system for transferring nucleic acids across the nuclear membrane
JP2003514564A (ja) * 1999-11-24 2003-04-22 エムシーエス マイクロ キャリア システムズ ゲーエムベーハー 核局在化シグナルまたはタンパク質導入領域の多量体を含むポリペプチド、および分子を細胞内へ移入するためのその使用法
EP1335933A2 (en) * 2000-11-24 2003-08-20 Pantheco A/S Pna analogues
AU2002216946A1 (en) * 2001-01-05 2002-07-16 Pantheco A/S Modified pna molecules
KR100912741B1 (ko) 2001-01-10 2009-08-18 아막사 아게 모듈식 트랜스펙션 시스템
WO2003092735A2 (en) * 2002-05-01 2003-11-13 Pantheco A/S Peptide nucleic acid conjugates with beta-amino acid polymers or reptoids
AU2003219576A1 (en) * 2003-04-03 2004-10-25 Korea Advanced Institute Of Science And Technology Conjugate for gene transfer comprising oligonucleotide and hydrophilic polymer, polyelectrolyte complex micelles formed from the conjugate, and methods for preparation thereof
US8969543B2 (en) * 2003-04-03 2015-03-03 Bioneer Corporation SiRNA-hydrophilic polymer conjugates for intracellular delivery of siRNA and method thereof
US20070149496A1 (en) * 2003-10-31 2007-06-28 Jack Tuszynski Water-soluble compound
CN101287835A (zh) * 2005-08-17 2008-10-15 株式会社百奥尼 用于siRNA细胞内输送的siRNA-亲水性聚合物结合物及其方法
EP1900820A1 (en) * 2006-09-13 2008-03-19 Amaxa AG Nuclear transport agent and method for producing said agent
WO2019219721A1 (en) 2018-05-18 2019-11-21 F. Hoffmann-La Roche Ag Targeted intracellular delivery of large nucleic acids

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
AU682308C (en) 1992-04-03 2006-08-17 Regents Of The University Of California, The Self-assembling polynucleotide delivery system
US5574142A (en) 1992-12-15 1996-11-12 Microprobe Corporation Peptide linkers for improved oligonucleotide delivery
US5631237A (en) 1992-12-22 1997-05-20 Dzau; Victor J. Method for producing in vivo delivery of therapeutic agents via liposomes
JPH10501681A (ja) * 1994-02-22 1998-02-17 ダナ−ファーバー キャンサー インスティチュート 核酸送達システムならびにその合成および使用方法
JPH10501963A (ja) * 1994-04-15 1998-02-24 ターゲテッド ジェネティクス コーポレイション 遺伝子送達融合タンパク質
US5670347A (en) * 1994-05-11 1997-09-23 Amba Biosciences Llc Peptide-mediated gene transfer
WO1996040961A1 (en) * 1995-06-07 1996-12-19 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections
DE19541679A1 (de) 1995-11-08 1997-05-15 Gerhard Dr Friedrich Nicht virale Gentransfersysteme mit NLS (nuclear localization sequences) und/oder magnetisierbaren Mikropartikeln und das damit verbundene Herstellungsverfahren
AUPN741696A0 (en) * 1996-01-05 1996-01-25 Commonwealth Scientific And Industrial Research Organisation Delivery of nucleic acids ii
WO1998028417A1 (en) * 1996-12-23 1998-07-02 Gene-Cell Nucleic acid constructs and uses thereof for direct nucleic acid incorporation into cells
AU7127598A (en) * 1997-04-18 1998-11-13 Millennium Pharmaceuticals, Inc. Novel molecules of the fthma-070-related protein family and the t85-related pro tein family and uses thereof
AU764660B2 (en) * 1999-01-08 2003-08-28 Lonza Cologne Ag Use of the cell's own transport system for transferring nucleic acids across the nuclear membrane
US6312956B1 (en) * 1999-10-01 2001-11-06 Vanderbilt University Nuclear targeted peptide nucleic acid oligomer

Also Published As

Publication number Publication date
JP2002534437A (ja) 2002-10-15
JP3997056B2 (ja) 2007-10-24
CN1339068A (zh) 2002-03-06
US6521456B1 (en) 2003-02-18
EP1141364A1 (de) 2001-10-10
WO2000040742A1 (de) 2000-07-13
AU764660B2 (en) 2003-08-28
CA2358040C (en) 2008-06-10
CA2358040A1 (en) 2000-07-13
IL143983A0 (en) 2002-04-21
JP2006345867A (ja) 2006-12-28
AU2533700A (en) 2000-07-24

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