CN1261563C - 人参愈伤组织细胞冻干及超低温保存方法 - Google Patents
人参愈伤组织细胞冻干及超低温保存方法 Download PDFInfo
- Publication number
- CN1261563C CN1261563C CN03120982.3A CN03120982A CN1261563C CN 1261563 C CN1261563 C CN 1261563C CN 03120982 A CN03120982 A CN 03120982A CN 1261563 C CN1261563 C CN 1261563C
- Authority
- CN
- China
- Prior art keywords
- cell
- protective material
- freeze
- ginseng callus
- drying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 31
- 206010020649 Hyperkeratosis Diseases 0.000 title claims abstract description 27
- 241000208340 Araliaceae Species 0.000 title claims abstract description 24
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 24
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 24
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 24
- 238000004108 freeze drying Methods 0.000 title claims abstract description 16
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 17
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 17
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 17
- 210000004027 cell Anatomy 0.000 claims description 52
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 39
- 239000000463 material Substances 0.000 claims description 36
- 230000001681 protective effect Effects 0.000 claims description 33
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 22
- 235000011187 glycerol Nutrition 0.000 claims description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 238000004321 preservation Methods 0.000 claims description 8
- 238000002560 therapeutic procedure Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 210000004748 cultured cell Anatomy 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 2
- 239000003223 protective agent Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 9
- 230000012010 growth Effects 0.000 description 7
- 238000005201 scrubbing Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000005138 cryopreservation Methods 0.000 description 5
- 235000020183 skimmed milk Nutrition 0.000 description 5
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004992 fission Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- JGSARLDLIJGVTE-UHFFFAOYSA-N 3,3-dimethyl-7-oxo-6-[(2-phenylacetyl)amino]-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Chemical compound O=C1N2C(C(O)=O)C(C)(C)SC2C1NC(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000022472 cold acclimation Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Toxicology (AREA)
- Agronomy & Crop Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03120982.3A CN1261563C (zh) | 2003-03-26 | 2003-03-26 | 人参愈伤组织细胞冻干及超低温保存方法 |
| PCT/CN2004/000264 WO2004084629A1 (fr) | 2003-03-26 | 2004-03-26 | Procede de lyophilisation et de cryoconservation de cals et cryoprotecteur utilise |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03120982.3A CN1261563C (zh) | 2003-03-26 | 2003-03-26 | 人参愈伤组织细胞冻干及超低温保存方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1532279A CN1532279A (zh) | 2004-09-29 |
| CN1261563C true CN1261563C (zh) | 2006-06-28 |
Family
ID=33035141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN03120982.3A Expired - Fee Related CN1261563C (zh) | 2003-03-26 | 2003-03-26 | 人参愈伤组织细胞冻干及超低温保存方法 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN1261563C (fr) |
| WO (1) | WO2004084629A1 (fr) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100424176C (zh) * | 2005-06-03 | 2008-10-08 | 中国科学院上海生命科学研究院 | 人参液泡膜水孔蛋白基因、其编码的氨基酸序列和应用 |
| CN1962852B (zh) * | 2006-11-27 | 2011-11-09 | 西北农林科技大学 | 红景天多糖在制备抗冻剂中的新用途及其产品和制备方法 |
| CN101037666B (zh) * | 2007-02-09 | 2010-04-21 | 浙江大学 | 一种动物细胞的低温保存溶液及保存方法 |
| CN102258008B (zh) * | 2011-05-31 | 2013-08-21 | 成都大学 | 一种从有效保存苦荞麦愈伤组织的玻璃化超低温保存方法 |
| CN102258010B (zh) * | 2011-09-02 | 2013-12-18 | 成都大学 | 一种华重楼根茎的慢速冷冻保存方法 |
| CN102578077B (zh) * | 2012-01-13 | 2013-07-24 | 成都美进生物科技有限公司 | 一种无血清低温保护剂 |
| CN102763591B (zh) * | 2012-07-05 | 2013-11-13 | 中国农业科学院作物科学研究所 | 一种白术的保存方法以及再培养方法 |
| CN103202225B (zh) * | 2013-03-11 | 2014-07-30 | 上海市农业生物基因中心 | 一种植物微茎尖超低温保存和解冻、恢复培养的方法 |
| CN103283716B (zh) * | 2013-06-08 | 2014-11-26 | 中南民族大学 | 一种简便的植物by-2细胞冷冻保存方法 |
| CN105766894B (zh) * | 2016-05-18 | 2018-10-19 | 中国农业科学院特产研究所 | 人参丛生芽超低温保存及植株再生培养方法 |
| CN115176708B (zh) * | 2022-08-12 | 2023-07-04 | 江苏省中国科学院植物研究所 | 一种落羽杉胚性愈伤组织的超低温冷冻保存方法 |
-
2003
- 2003-03-26 CN CN03120982.3A patent/CN1261563C/zh not_active Expired - Fee Related
-
2004
- 2004-03-26 WO PCT/CN2004/000264 patent/WO2004084629A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| CN1532279A (zh) | 2004-09-29 |
| WO2004084629A1 (fr) | 2004-10-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Changchun Keygen Biological Products Co., Ltd. Assignor: Changchun Research Institute of Biological Products Contract record no.: 2011220000024 Denomination of invention: Method for freeze-drying and ultra low temperature storing ginseng callus cell Granted publication date: 20060628 License type: Exclusive License Open date: 20040929 Record date: 20110801 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060628 Termination date: 20150326 |
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| EXPY | Termination of patent right or utility model |