CN1252258C - 具有改进的卵污渍洗涤性能的新枯草杆菌酶 - Google Patents
具有改进的卵污渍洗涤性能的新枯草杆菌酶 Download PDFInfo
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- 239000007787 solid Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 150000003457 sulfones Chemical class 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000005494 tarnishing Methods 0.000 description 1
- 108010075550 termamyl Proteins 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- RYCLIXPGLDDLTM-UHFFFAOYSA-J tetrapotassium;phosphonato phosphate Chemical compound [K+].[K+].[K+].[K+].[O-]P([O-])(=O)OP([O-])([O-])=O RYCLIXPGLDDLTM-UHFFFAOYSA-J 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- 230000009974 thixotropic effect Effects 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 101150052264 xylA gene Proteins 0.000 description 1
- 101150110790 xylB gene Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21062—Subtilisin (3.4.21.62)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
BLSAVI | BLAP | BASBPN | BLSCAR | SEQ ID NO:2 | |
BLSAVIBLAP1)BASBPNBLSCARSEQ ID NO:2 | 10098.160.460.986.6 | 10060.761.387.4 | 10069.559.3 | 10055.8 | 100 |
直链C12烷基苯磺酸钠 | 6.5 |
硫酸钠沸石A次氮基三乙酸钠酶PVPTAED硼酸过硼酸盐苯酚磺酸盐次要组分 | 15.026.05.00.10.53.04.018.00.1补充至100% |
45AS25E3S25E525E3TFAA沸石ANaSKS-6柠檬酸碳酸盐MA/AACMC酶TAED过碳酸盐EDDS | 8.02.03.03.02.517.012.03.07.05.00.40.16.022.00.3 |
粒状抑泡剂水/次要组分 | 3.5补充至100% |
LASTASTFAA45AS45E725E3S68E1125E5柠檬酸盐碳酸盐柠檬酸沸石ANa-SKS-6MA/AADETPMP酶硅酸盐硫酸盐PVP聚(4-乙烯基吡啶)-N-氧化物/乙烯基咪唑和乙烯基吡咯烷酮的共聚物过硼酸盐 | 10.72.4-3.14.0-1.8-15.0-2.532.1-5.00.20.102.55.20.5-1.0 | --4.010.0-3.0-8.07.010.03.025.09.05.00.80.05-3.0-0.2- |
苯酚磺酸盐 | 0.2 | - |
水/次要组分 | 补充至100% |
45ASLAS68AS45E725E3椰油-烷基-二甲基羟基乙基氯化铵柠檬酸盐Na-SKS-6沸石AMA/AADETPMP过硼酸盐过碳酸盐TAED绿土黏土HMWPEO酶硅酸盐碳酸盐粒状抑泡剂CMC | -7.61.34.0-1.45.0-15.04.00.415.0-5.010.0-0.103.010.01.00.2 | 10.0---5.01.03.011.015.04.00.4-15.05.010.00.10.055.010.04.00.1 |
水/次要组分 | 补充至100% |
酸式LAS柠檬酸酸式25AS酸式25AE2S25AE7CFAADETPMP脂肪酸油酸乙醇丙二醇酶椰油-烷基二甲基羟基乙基氯化铵绿土黏土PVP | -5.08.03.08.051.08-4.02.00.10--2.0 | 25.02.0----1.0-1.06.06.00.053.05.0- |
水/次要组分 | 补充至100% |
非离子表面活性剂 | 0.4-2.5% |
硅酸钠 | 0-20% |
二硅酸钠 | 3-20% |
三磷酸钠 | 20-40% |
碳酸钠 | 0-20% |
过硼酸钠 | 2-9% |
四乙酰基乙二胺(TAED) | 1-4% |
硫酸钠 | 5-33% |
酶 | 0.0001-0.1% |
非离子表面活性剂(如脂肪醇乙氧基化物) | 1-2% |
二硅酸钠 | 2-30% |
碳酸钠 | 10-50% |
磷酸钠 | 0-5% |
柠檬酸三钠二水合物 | 9-30% |
乙酸次氮基三钠(NTA) | 0-20% |
过硼酸钠一水合物 | 5-10% |
四乙酰基乙二胺(TAED) | 1-2% |
聚丙烯酸盐聚合物(例如马来酸/丙烯酸共聚物) | 6-25% |
酶 | 0.0001-0.1% |
香料 | 0.1-0.5% |
水 | 5-10 |
非离子表面活性剂 | 0.5-2.0% |
二硅酸钠 | 25-40% |
柠檬酸钠 | 30-55% |
碳酸钠 | 0-29% |
碳酸氢钠 | 0-20% |
一水合过硼酸钠 | 0-15% |
四乙酰基乙二胺(TAED) | 0-6% |
马来酸/丙烯酸共聚物 | 0-5% |
黏土 | 1-3% |
聚氨基酸 | 0-20% |
聚丙烯酸钠 | 0-8% |
酶 | 0.0001-0.1% |
非离子表面活性剂 | 1-2% |
沸石MAP | 15-42% |
二硅酸钠 | 30-34% |
柠檬酸钠 | 0-12% |
碳酸钠 | 0-20% |
过硼酸钠一水合物 | 7-15% |
四乙酰基乙二胺(TAED) | 0-3% |
多聚物 | 0-4% |
马来酸/丙烯酸共聚物 | 0-5% |
有机磷酸脂 | 0-4% |
黏土 | 1-2% |
酶 | 0.0001-0.1% |
硫酸钠 | 平衡 |
非离子表面活性剂 | 1-7% |
二硅酸钠 | 18-30% |
柠檬酸三钠 | 10-24% |
碳酸钠 | 12-20% |
单过硫酸盐(2KHSO5·KHSO4·K2SO4) | 15-21% |
漂白稳定剂 | 0.1-2% |
马来酸/丙烯酸共聚物 | 0-6% |
二亚乙基三胺五乙酸五钠盐 | 0-2.5% |
酶 | 0.0001-0.1% |
硫酸钠,水 | 平衡 |
非离子表面活性剂 | 0-1.5% |
十八烷基二甲基胺N-氧化物二水合物 | 0-5% |
十八烷基二甲基胺N-氧化物二水合物和十六烷基二甲基胺N-氧化物二水合物的80∶20wt.C18/C16掺混物 | 0-4% |
无水十八烷基双(羟乙基)N-氧化物和无水十六烷基双(羟乙基)胺N-氧化物的70∶30wt.C18/C16掺混物 | 0-5% |
平均乙氧基化程度为3的C13-C15烷基乙氧基硫酸酯 | 0-10% |
平均乙氧基化程度为3的C12-C15烷基乙氧基硫酸酯 | 0-5% |
平均乙氧基化程度为12的C13-C15乙氧基化的醇 | 0-5% |
平均乙氧基化程度为9的C12-C15乙氧基化醇混合物 | 0-6.5% |
平均乙氧基化程度为30的C13-C15乙氧基化醇 | 0-4% |
混合物 | |
二硅酸钠 | 0-33% |
三磷酸钠 | 0-46% |
柠檬酸钠 | 0-28% |
柠檬酸 | 0-29% |
碳酸钠 | 0-20% |
过硼酸钠一水合物 | 0-11.5% |
四乙酰基乙二胺(TAED) | 0-4% |
马来酸/丙烯酸共聚物 | 0-7.5% |
硫酸钠 | 0-12.5% |
酶 | 0.0001-0.1% |
液体非离子表面活性剂(例如脂肪醇乙氧基化物) | 2.0-10.0% |
碱金属硅酸盐 | 3.0-15.0% |
碱金属磷酸盐 | 20.0-40.0% |
选自高级二元醇、聚乙二醇、多氧化物、乙二醇醚的液体载体 | 25.0-45.0% |
稳定剂(例如磷酸和C16-C18链烷醇的不完全酯) | 0.5-7.0% |
泡沫抑制剂(例如硅氧烷) | 0-1.5% |
酶 | 0.0001-0.1% |
液体非离子表面活性剂(例如脂肪醇乙氧基化物) | 2.0-10.0% |
硅酸钠 | 3.0-15.0% |
碱金属碳酸盐 | 7.0-20.0% |
柠檬酸钠 | 0.0-1.5% |
稳定系统(例如细粒硅氧烷和低分子量二烷基聚乙二醇醚的混合物) | 0.5-7.0% |
低分子量聚丙烯酸酯聚合物 | 5.0-15.0% |
黏土凝胶增稠剂(例如斑脱土) | 0.0-10.0% |
羟丙基纤维素聚合物 | 0.0-0.6% |
酶 | 0.0001-0.1% |
选自高级二元醇、聚乙二醇、多氧化物和乙二醇醚的液体载体 | 平衡量 |
C12-C14脂肪酸 | 0-0.5% |
嵌段共聚物表面活性剂 | 1.5-15.0% |
柠檬酸钠 | 0-12% |
三磷酸钠 | 0-15% |
碳酸钠 | 0-8% |
三硬脂酸铝 | 0-0.1% |
枯烯磺酸钠 | 0-1.7% |
聚丙烯酸盐增稠剂 | 1.32-2.5% |
聚丙烯酸钠 | 2.4-6.0% |
硼酸 | 0-4.0% |
甲酸钠 | 0-0.45% |
甲酸钙 | 0-0.2% |
正癸基二苯基氧化物二磺酸钠 | 0-4.0% |
一乙醇胺(MEA) | 0-1.86% |
氢氧化钠(50%) | 1.9-9.3% |
1,2-丙二醇 | 0-9.4% |
酶 | 0.0001-0.1% |
抑泡剂、染料、香料、水 | 平衡量 |
脂肪醇乙氧基化物 | 0-20% |
脂肪酸酯磺酸酯 | 0-30% |
十二烷基硫酸钠 | 0-20% |
烷基多苷 | 0-21% |
油酸 | 0-10% |
二硅酸钠一水合物 | 18-33% |
柠檬酸钠二水合物 | 18-33% |
硬脂酸钠 | 0-2.5% |
过硼酸钠一水合物 | 0-13% |
四乙酰基乙二胺(TAED) | 0-8% |
马来酸/丙烯酸共聚物 | 4-8% |
酶 | 0.0001-0.1% |
硅酸钠 | 5-10% |
焦磷酸四钾 | 15-25% |
三磷酸钠 | 0-2% |
碳酸钾 | 4-8% |
被保护的漂白颗粒,如氯 | 5-10% |
聚合增稠剂 | 0.7-1.5% |
氢氧化钾 | 0-2% |
酶 | 0.0001-0.1% |
水 | 平衡 |
酶 | R(460nm) | P |
空白(没有酶) | 40.5 | - |
Savinase | 40.7 | - |
SEQ ID NO:2 | 46.5 | 5.8 |
酶 | %RPF(20nM) | %RPF(40nm) |
Savinase | 3.9±1.6 | 3.0±1.0 |
SEQ ID NO:2 | 42.4±16.5 | 78.7±2.1 |
保藏物 | 保藏号 | 保藏日期 |
大肠杆菌 MT173 | DSM 13306 | 2000年2月10日 |
Claims (34)
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DKPA200000405 | 2000-03-14 | ||
DKPA200000405 | 2000-03-14 |
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US (1) | US6777218B1 (zh) |
EP (1) | EP1272623B1 (zh) |
JP (1) | JP4927286B2 (zh) |
CN (1) | CN1252258C (zh) |
AT (1) | ATE315640T1 (zh) |
AU (2) | AU2001239214B8 (zh) |
CA (1) | CA2402866C (zh) |
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DK6488D0 (da) * | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
US6682924B1 (en) * | 1995-05-05 | 2004-01-27 | Novozymes A/S | Protease variants and compositions |
US20120165241A1 (en) * | 1995-05-05 | 2012-06-28 | Unilever Plc | Subtilase Variants |
WO2001075087A2 (en) * | 2000-04-03 | 2001-10-11 | Maxygen, Inc. | Subtilisin variants |
AU2001254623A1 (en) * | 2000-04-28 | 2001-11-12 | Novozymes A/S | Production and use of protein variants having modified immunogenicity |
DE10162728A1 (de) | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
DE10162727A1 (de) * | 2001-12-20 | 2003-07-10 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease |
JP4703113B2 (ja) * | 2002-01-16 | 2011-06-15 | ジェネンコー・インターナショナル・インク | 複数置換プロテアーゼ変異体 |
ATE407202T1 (de) * | 2003-05-07 | 2008-09-15 | Novozymes As | Enzymvarianten von subtilisin (subtilasen) |
EP2013339B1 (en) | 2006-04-20 | 2013-09-11 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
KR20090031906A (ko) * | 2006-07-18 | 2009-03-30 | 다니스코 유에스 인크. | 광범위한 온도에 걸쳐 활성인 프로테아제 변이형 |
US8034766B2 (en) * | 2006-10-27 | 2011-10-11 | E I Du Pont De Nemours And Company | Compositions and methods for prion decontamination |
FI121851B (fi) * | 2009-07-08 | 2011-05-13 | Ab Enzymes Oy | Sieniperäinen proteaasi ja sen käyttö |
DE102011005354A1 (de) | 2011-03-10 | 2012-09-13 | Henkel Ag & Co. Kgaa | Leistungsverbesserte Proteasevarianten |
US10683491B2 (en) * | 2014-12-04 | 2020-06-16 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2016096714A1 (en) * | 2014-12-15 | 2016-06-23 | Henkel Ag & Co. Kgaa | Detergent composition comprising subtilase variants |
KR20200023455A (ko) | 2017-06-30 | 2020-03-04 | 코덱시스, 인코포레이티드 | T7 rna 폴리머라제 변이체 |
US10738286B2 (en) | 2017-06-30 | 2020-08-11 | Codexis, Inc. | T7 RNA polymerase variants |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
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---|---|---|---|---|
GB1519148A (en) * | 1974-11-19 | 1978-07-26 | Gist Brocades Nv | Compositions of matter |
NZ208612A (en) | 1983-06-24 | 1991-09-25 | Genentech Inc | Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations |
US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
DE3527913A1 (de) | 1985-08-03 | 1987-02-12 | Henkel Kgaa | Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen |
WO1987004461A1 (en) | 1986-01-15 | 1987-07-30 | Amgen | THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF |
JPS63502959A (ja) | 1986-02-12 | 1988-11-02 | ジェネックス、コ−ポレ−ション | 突然変異誘発及びスクリ−ニング方法並びに生成物 |
IE65767B1 (en) | 1986-04-30 | 1995-11-15 | Genencor Int | Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors |
NZ221627A (en) | 1986-09-09 | 1993-04-28 | Genencor Inc | Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios |
JP3026567B2 (ja) * | 1987-02-27 | 2000-03-27 | ジェネンコー インターナショナル インコーポレイテッド | 分子のクローニング及びタンパク分解酵素をコードする遺伝子の発現 |
ATE184316T1 (de) | 1987-04-06 | 1999-09-15 | Novo Nordisk As | Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen |
US4914031A (en) | 1987-04-10 | 1990-04-03 | Amgen, Inc. | Subtilisin analogs |
DK6488D0 (da) | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
CN1056187C (zh) * | 1988-02-11 | 2000-09-06 | 金克克国际有限公司 | 新的蛋白水解酶及其在洗涤剂中的应用 |
US6287841B1 (en) * | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
US5324653A (en) * | 1988-02-11 | 1994-06-28 | Gist-Brocades N.V. | Recombinant genetic means for the production of serine protease muteins |
US5543302A (en) | 1988-05-27 | 1996-08-06 | Solvay Enzymes, Inc. | Proteases of altered stability to autolytic degradation |
EP0405901B1 (en) * | 1989-06-26 | 2004-09-01 | Unilever Plc | Enzymatic detergent compositions |
DK316989D0 (da) | 1989-06-26 | 1989-06-26 | Novo Nordisk As | Enzymer |
US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
JP3220137B2 (ja) * | 1989-08-25 | 2001-10-22 | ヘンケル・リサーチ・コーポレイション | アルカリ性タンパク質分解酵素およびその製造方法 |
US5352603A (en) * | 1989-08-31 | 1994-10-04 | Kali-Chemie Ag | Highly alkaline proteases |
US6271012B1 (en) * | 1989-10-11 | 2001-08-07 | Genencor International, Inc. | Protease muteins and their use in detergents |
DK97190D0 (da) * | 1990-04-19 | 1990-04-19 | Novo Nordisk As | Oxidationsstabile detergentenzymer |
DK271490D0 (da) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | Detergentkomposition |
US5482849A (en) * | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
EP0583339B1 (en) * | 1991-05-01 | 1998-07-08 | Novo Nordisk A/S | Stabilized enzymes and detergent compositions |
US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
EP0995801A1 (de) | 1991-07-27 | 2000-04-26 | Genencor International GmbH | Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme |
PL178045B1 (pl) | 1992-07-17 | 2000-02-29 | Genencor Int | Mutant proteazy do stosowania w detergentach, sekwencja DNA kodująca mutanta proteazy, sposób otrzymywania mutanta proteazy oraz kompozycja detergentowa |
US6436690B1 (en) | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
DE69432954T2 (de) * | 1993-10-14 | 2004-04-22 | The Procter & Gamble Company, Cincinnati | Proteasen enthaltende bleichmittelzusammensetzungen |
MA23346A1 (fr) | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
AU8079794A (en) * | 1993-10-14 | 1995-05-04 | Procter & Gamble Company, The | Protease-containing cleaning compositions |
ES2364774T3 (es) * | 1994-02-24 | 2011-09-14 | HENKEL AG & CO. KGAA | Enzimas mejoradas y detergentes que las contienen. |
US5691295A (en) | 1995-01-17 | 1997-11-25 | Cognis Gesellschaft Fuer Biotechnologie Mbh | Detergent compositions |
DE4411223A1 (de) | 1994-03-31 | 1995-10-05 | Solvay Enzymes Gmbh & Co Kg | Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren |
US6599730B1 (en) | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
ZA952220B (en) | 1994-05-02 | 1995-12-14 | Procter & Gamble | Bpn' variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
US5837517A (en) * | 1995-05-05 | 1998-11-17 | Novo Nordisk A/S | Protease variants and compositions |
CA2270180C (en) * | 1996-11-04 | 2011-01-11 | Novo Nordisk A/S | Subtilase variants and compositions |
EP0985042B1 (en) | 1997-06-04 | 2006-09-06 | Genecor International, Inc. | Protease enzymes for tough cleaning and/or spot and film reduction and compositions incorporating same |
MA25044A1 (fr) | 1997-10-23 | 2000-10-01 | Procter & Gamble | Compositions de lavage contenant des variants de proteases multisubstituees. |
EP1032655B1 (en) | 1997-11-21 | 2005-06-29 | Novozymes A/S | Protease variants and compositions |
JP4768128B2 (ja) * | 1998-12-18 | 2011-09-07 | ノボザイムス アクティーゼルスカブ | 活性部位ループ領域中に追加のアミノ酸残基を有するサブチラーゼ酵素サブグループi−s1及びi−s2 |
BRPI9916351B1 (pt) * | 1998-12-18 | 2016-04-12 | Novozymes As | enzima de subtilase isolada, composição, e, uso de uma enzima subtilase isolada ou de uma composição de enzima |
JP2003516751A (ja) * | 1999-12-15 | 2003-05-20 | ノボザイムス アクティーゼルスカブ | 卵の染みに及ぼす洗浄性能改良を示すズブチラーゼ変異体 |
-
2000
- 2000-03-23 US US09/533,480 patent/US6777218B1/en not_active Expired - Fee Related
-
2001
- 2001-03-12 EP EP01913739A patent/EP1272623B1/en not_active Expired - Lifetime
- 2001-03-12 CA CA2402866A patent/CA2402866C/en not_active Expired - Fee Related
- 2001-03-12 CN CNB018065317A patent/CN1252258C/zh not_active Expired - Fee Related
- 2001-03-12 AU AU2001239214A patent/AU2001239214B8/en not_active Ceased
- 2001-03-12 WO PCT/DK2001/000163 patent/WO2001068821A2/en active IP Right Grant
- 2001-03-12 AU AU3921401A patent/AU3921401A/xx active Pending
- 2001-03-12 AT AT01913739T patent/ATE315640T1/de not_active IP Right Cessation
- 2001-03-12 JP JP2001567305A patent/JP4927286B2/ja not_active Expired - Fee Related
- 2001-03-12 DE DE60116605T patent/DE60116605T2/de not_active Expired - Lifetime
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ATE315640T1 (de) | 2006-02-15 |
CA2402866C (en) | 2016-02-09 |
WO2001068821A3 (en) | 2002-04-04 |
EP1272623A2 (en) | 2003-01-08 |
CN1418249A (zh) | 2003-05-14 |
AU2001239214B2 (en) | 2006-06-08 |
AU3921401A (en) | 2001-09-24 |
DE60116605T2 (de) | 2006-09-14 |
JP4927286B2 (ja) | 2012-05-09 |
WO2001068821A2 (en) | 2001-09-20 |
JP2003526363A (ja) | 2003-09-09 |
AU2001239214B8 (en) | 2006-07-20 |
DE60116605D1 (de) | 2006-04-06 |
US6777218B1 (en) | 2004-08-17 |
CA2402866A1 (en) | 2001-09-20 |
EP1272623B1 (en) | 2006-01-11 |
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