CN1238807A - 稳定的瞬时基因表达 - Google Patents
稳定的瞬时基因表达 Download PDFInfo
- Publication number
- CN1238807A CN1238807A CN97180186A CN97180186A CN1238807A CN 1238807 A CN1238807 A CN 1238807A CN 97180186 A CN97180186 A CN 97180186A CN 97180186 A CN97180186 A CN 97180186A CN 1238807 A CN1238807 A CN 1238807A
- Authority
- CN
- China
- Prior art keywords
- cell
- reagent
- acid
- dna
- transient expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7135—Compounds containing heavy metals
- A61K31/714—Cobalamins, e.g. cyanocobalamin, i.e. vitamin B12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/131—Amines acyclic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
- A61K31/136—Amines having aromatic rings, e.g. ketamine, nortriptyline having the amino group directly attached to the aromatic ring, e.g. benzeneamine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
- A61K31/137—Arylalkylamines, e.g. amphetamine, epinephrine, salbutamol, ephedrine or methadone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/192—Carboxylic acids, e.g. valproic acid having aromatic groups, e.g. sulindac, 2-aryl-propionic acids, ethacrynic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
- A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/205—Amine addition salts of organic acids; Inner quaternary ammonium salts, e.g. betaine, carnitine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4164—1,3-Diazoles
- A61K31/4172—Imidazole-alkanecarboxylic acids, e.g. histidine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Emergency Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3010996P | 1996-11-01 | 1996-11-01 | |
US60/030,109 | 1996-11-01 | ||
US83374797A | 1997-04-11 | 1997-04-11 | |
US08/833,747 | 1997-04-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN1238807A true CN1238807A (zh) | 1999-12-15 |
Family
ID=26705677
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN97180186A Pending CN1238807A (zh) | 1996-11-01 | 1997-10-31 | 稳定的瞬时基因表达 |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0941356A2 (fr) |
JP (1) | JP2000509998A (fr) |
CN (1) | CN1238807A (fr) |
AU (1) | AU732445B2 (fr) |
CA (1) | CA2270137A1 (fr) |
WO (1) | WO1998020146A2 (fr) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533722A (zh) * | 2010-12-24 | 2012-07-04 | 北京义翘神州生物技术有限公司 | 一种通过温度突变提高哺乳动物细胞重组蛋白瞬时表达的方法 |
CN106811483A (zh) * | 2017-02-16 | 2017-06-09 | 甘肃农业大学 | 一种高效转染真核细胞的方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4036784A1 (de) * | 1990-11-17 | 1992-05-21 | Behringwerke Ag | Antivirale aktivitaet des vom adeno-assoziierten virus typ 2 codierten rep-gens |
ATE302854T1 (de) * | 1993-01-26 | 2005-09-15 | Univ Pennsylvania | Zusammensetzungen und verfahren zur verabreichung von genetischem material |
US5739118A (en) * | 1994-04-01 | 1998-04-14 | Apollon, Inc. | Compositions and methods for delivery of genetic material |
DK0815257T3 (da) * | 1995-01-31 | 2002-04-22 | Bioimage As | Fremgangsmåde til karakterisering af biologisk aktive stoffer |
-
1997
- 1997-10-31 JP JP10521611A patent/JP2000509998A/ja active Pending
- 1997-10-31 EP EP97946432A patent/EP0941356A2/fr not_active Withdrawn
- 1997-10-31 CN CN97180186A patent/CN1238807A/zh active Pending
- 1997-10-31 AU AU51601/98A patent/AU732445B2/en not_active Ceased
- 1997-10-31 CA CA002270137A patent/CA2270137A1/fr not_active Abandoned
- 1997-10-31 WO PCT/US1997/019860 patent/WO1998020146A2/fr not_active Application Discontinuation
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533722A (zh) * | 2010-12-24 | 2012-07-04 | 北京义翘神州生物技术有限公司 | 一种通过温度突变提高哺乳动物细胞重组蛋白瞬时表达的方法 |
CN102533722B (zh) * | 2010-12-24 | 2014-03-12 | 北京义翘神州生物技术有限公司 | 一种通过温度突变提高哺乳动物细胞重组蛋白瞬时表达的方法 |
CN106811483A (zh) * | 2017-02-16 | 2017-06-09 | 甘肃农业大学 | 一种高效转染真核细胞的方法 |
CN106811483B (zh) * | 2017-02-16 | 2020-04-07 | 甘肃农业大学 | 一种高效转染真核细胞的方法 |
Also Published As
Publication number | Publication date |
---|---|
AU732445B2 (en) | 2001-04-26 |
WO1998020146A2 (fr) | 1998-05-14 |
EP0941356A2 (fr) | 1999-09-15 |
JP2000509998A (ja) | 2000-08-08 |
WO1998020146A3 (fr) | 1998-08-20 |
CA2270137A1 (fr) | 1998-05-14 |
AU5160198A (en) | 1998-05-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20230272346A1 (en) | Compositions and methods for increasing the culture density of a cellular biomass within a cultivation infrastructure | |
CN1271992C (zh) | 作为遗传物质转移系统的阳离子病毒体 | |
CN103930542A (zh) | 棕色脂肪细胞组合物及方法 | |
CN1728946A (zh) | 获得活的人肝细胞,包括肝干/祖细胞的方法 | |
CN1071333A (zh) | 钙受体活性分子 | |
CN1174512A (zh) | 蛋氨酸酶在抗蛋氨酸和抗高半胱氨酸的化学治疗中的应用 | |
CN1270630A (zh) | 处理白细胞的方法、白细胞组合物及其应用方法 | |
CN1084564A (zh) | 反义多核苷酸 | |
CN102864171B (zh) | 特异促进肝细胞cyp3a4基因高表达的慢病毒表达载体及其构建方法与应用 | |
CN1306569A (zh) | 稳定化的瞬时基因表达 | |
CN102766654A (zh) | 特异促进肝细胞cyp1a2基因高表达的慢病毒表达载体及其构建方法与应用 | |
Salzman | Animal Cell Cultures: Tissue culture is a powerful tool in the study of nutrition, physiology, virology, and genetics. | |
CN102876716B (zh) | 特异促进肝细胞cyp2e1基因高表达的慢病毒表达载体及其构建方法与应用 | |
CN105039251A (zh) | 一种pH值稳定的淋巴细胞无血清培养基及其制备方法和应用 | |
CN1238807A (zh) | 稳定的瞬时基因表达 | |
Marzullo | Regulation of cartilage enzymes in cultured chondrocytes and the effect of 5-bromodeoxyuridine | |
CN105695400A (zh) | 一种纯化干细胞来源的心肌细胞的无血清培养基 | |
CN104164404A (zh) | 一种高效的体外培养人脐带间质干细胞的无血清培养体系的用途 | |
CN103243094B (zh) | 布莱凯特黑牛pdss1基因单核苷酸多态性及其检测方法 | |
CN1686566A (zh) | Cark基因在制备治疗心肌肥厚药物中的应用及一种抑制心肌肥厚的药物 | |
CN109321574A (zh) | 抑制ILT5表达的短发卡shRNA、慢病毒及其应用 | |
CN1668339A (zh) | 类固醇缀合物、其制剂及其用途 | |
Bachelet et al. | Co-culture of mast cells with fibroblasts: a tool to study their crosstalk | |
CN1242749C (zh) | CoCl2在制备治疗白血病的药物中的应用 | |
CN1949979A (zh) | 用于改变生物细胞内多聚不饱和脂肪酸含量的组合物及方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |