CN1190495C - 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法 - Google Patents

在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法 Download PDF

Info

Publication number
CN1190495C
CN1190495C CNB998081124A CN99808112A CN1190495C CN 1190495 C CN1190495 C CN 1190495C CN B998081124 A CNB998081124 A CN B998081124A CN 99808112 A CN99808112 A CN 99808112A CN 1190495 C CN1190495 C CN 1190495C
Authority
CN
China
Prior art keywords
gene
cell
mutant
nucleic acid
fusarium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB998081124A
Other languages
English (en)
Chinese (zh)
Other versions
CN1309711A (zh
Inventor
J·C·罗耶
L·M·克里斯琴森
G·A·甘贝塔
H·布鲁迪
S·M·奥塔尼
W·T·约德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk Biotech Inc
Novozymes Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc, Novozymes Biotech Inc filed Critical Novo Nordisk Biotech Inc
Publication of CN1309711A publication Critical patent/CN1309711A/zh
Application granted granted Critical
Publication of CN1190495C publication Critical patent/CN1190495C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
CNB998081124A 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法 Expired - Fee Related CN1190495C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8221798A 1998-05-20 1998-05-20
US09/082,217 1998-05-20

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CNB031588026A Division CN1231579C (zh) 1998-05-20 1999-05-20 trichodiene合成酶及其制备方法

Publications (2)

Publication Number Publication Date
CN1309711A CN1309711A (zh) 2001-08-22
CN1190495C true CN1190495C (zh) 2005-02-23

Family

ID=22169802

Family Applications (2)

Application Number Title Priority Date Filing Date
CNB998081124A Expired - Fee Related CN1190495C (zh) 1998-05-20 1999-05-20 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法
CNB031588026A Expired - Fee Related CN1231579C (zh) 1998-05-20 1999-05-20 trichodiene合成酶及其制备方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CNB031588026A Expired - Fee Related CN1231579C (zh) 1998-05-20 1999-05-20 trichodiene合成酶及其制备方法

Country Status (9)

Country Link
US (3) US6180366B1 (enExample)
EP (1) EP1078081B1 (enExample)
JP (2) JP2002515253A (enExample)
CN (2) CN1190495C (enExample)
AT (1) ATE395424T1 (enExample)
AU (1) AU4195099A (enExample)
DE (1) DE69938722D1 (enExample)
DK (1) DK1078081T3 (enExample)
WO (1) WO1999060137A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102695787A (zh) * 2009-08-05 2012-09-26 诺维信公司 用于从木素纤维素原料产生trichodiene的丝状真菌和方法

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6060305A (en) * 1994-06-30 2000-05-09 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system
ATE466603T1 (de) * 1998-04-27 2010-05-15 Surmodics Inc Bioaktive wirkstoffe freisetzende beschichtungen
AU4195099A (en) * 1998-05-20 1999-12-06 Novo Nordisk Biotech, Inc. Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
FR2826978B1 (fr) * 2001-07-06 2004-09-17 Agronomique Inst Nat Rech Procede de determination de la capacite de fusarium a produire des toxines trichotecenes
US8148155B2 (en) * 2002-04-22 2012-04-03 Novozymes, Inc. Methods for increasing homologous recombination of a nucleic acid sequence
DK1546302T3 (da) * 2002-08-30 2009-12-14 Novozymes Inc Fremgangsmåder til fremstilling af pattedyrs-trypsiner
AU2003258395A1 (en) * 2002-08-30 2004-03-19 Puratos Naamloze Vennootschap Myrothecium sp. transformation and expression system
JP4490284B2 (ja) 2002-11-18 2010-06-23 ノボザイムス，インコーポレイティド 菌類細胞において遺伝子を発現するためのプロモーター変異体
US20090233287A1 (en) * 2006-04-25 2009-09-17 Roelof Ary Lans Bovenberg Production of compounds in a recombinant host
RU2008149928A (ru) * 2006-05-18 2010-06-27 Конинклейке Филипс Электроникс Н.В. (Nl) Горизонтальное тостерное устройство с относительно малыми верхними нагревательными средствами
CN102227502B (zh) 2008-09-30 2016-01-13 诺维信股份有限公司 在镶片镰孢的酶缺陷突变体中产生多肽的方法
US20100316764A1 (en) * 2009-06-10 2010-12-16 Engrain, LLC Flour supplement compositions and methods for preparing wheat flour
BR112012013205B1 (pt) 2009-12-18 2020-11-03 Novozymes, Inc. mutante de uma cepa parental de trichoderma reesei, e, métodos para produzir um polipeptídeo de interesse e para obter um mutante de uma cepa parental de trichoderma
CN108588060B (zh) * 2017-03-07 2020-12-01 武汉康复得生物科技股份有限公司 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶
JP2021078354A (ja) 2018-03-08 2021-05-27 有限会社アルティザイム・インターナショナル フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質
CN112522231B (zh) * 2020-12-14 2023-03-14 广东省微生物研究所（广东省微生物分析检测中心） 一种酰基转移酶及其编码基因和应用
WO2023088791A1 (en) 2021-11-22 2023-05-25 Basf Se Paenibacillus strains producing low amounts of dab comprising compounds.

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
WO1996000787A1 (en) * 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
EP1975237B1 (en) * 1996-03-11 2012-12-26 Meiji Co., Ltd. Beta-fructofuranosidase variants and uses thereof
AU4195099A (en) * 1998-05-20 1999-12-06 Novo Nordisk Biotech, Inc. Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
US6383781B1 (en) * 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102695787A (zh) * 2009-08-05 2012-09-26 诺维信公司 用于从木素纤维素原料产生trichodiene的丝状真菌和方法

Also Published As

Publication number Publication date
CN1231579C (zh) 2005-12-14
WO1999060137A2 (en) 1999-11-25
DK1078081T3 (da) 2008-08-04
WO1999060137A3 (en) 2000-05-18
US20050181480A1 (en) 2005-08-18
EP1078081B1 (en) 2008-05-14
US20080213901A1 (en) 2008-09-04
DE69938722D1 (de) 2008-06-26
JP2002515253A (ja) 2002-05-28
JP2009291207A (ja) 2009-12-17
CN1309711A (zh) 2001-08-22
US7332341B2 (en) 2008-02-19
CN1500882A (zh) 2004-06-02
ATE395424T1 (de) 2008-05-15
US6180366B1 (en) 2001-01-30
AU4195099A (en) 1999-12-06
EP1078081A2 (en) 2001-02-28

Similar Documents

Publication Publication Date Title
CN1190495C (zh) 在单端孢菌毒素缺陷的丝状真菌突变细胞中生产异源多肽的方法
CN1262639C (zh) 新的宿主细胞和生产蛋白质的方法
CN1165614C (zh) 含有木聚糖酶的动物饲料添加剂
CN1230546C (zh) 丝状真菌宿主领域的转化系统
CN1536089A (zh) 用于在真菌细胞中表达基因的启动子
CN1253574C (zh) 来自米曲霉的areA基因和其中areA基因已被修饰的真菌
CN1426470A (zh) Talaromyces emersonii的β－葡聚糖酶
CN1192106C (zh) 分离突变体及克隆互补基因的新方法
CN100344765C (zh) 用于调节转录的dna序列
CN1509330A (zh) 具有纤维二糖酶活性的多肽和编码其的多核苷酸
CN1344315A (zh) 改进的植酸酶
CN1273601A (zh) 酶混合物
CN1454259A (zh) 篮霉菌木聚糖酶
CN1051058A (zh) 微生物植酸酶的克隆及表达
CN1798848A (zh) 在缺乏酶的黑曲霉突变体中生产生物物质的方法
CN1118131A (zh) 一种酶饲料添加剂和包含它的动物饲料
CN1816627A (zh) 生产葡糖淀粉酶的方法及其用途
CN1156570C (zh) 微生物木糖葡聚糖内切转糖基酶、其生产方法及用途
CN1274820C (zh) 一种具有半乳聚糖酶活性的酶
CN1195058C (zh) 草酰乙酸水解酶缺陷型真菌宿主细胞
CN1160465C (zh) 其中areA、pepC和/或pepE基因已被灭活的真菌
CN1198939C (zh) 来自绿色木霉的纤维素酶cbh1基因的调控序列以及利用该序列的蛋白质或多肽的大量生产体系
CN1611601A (zh) 三肽基氨肽酶
CN1769423A (zh) 表达降低水平的金属蛋白酶的宿主细胞和在蛋白质产生中使用该宿主细胞的方法
CN1780917A (zh) 用于在真菌细胞中表达基因的启动子变体

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee

Owner name: NUOWOQIMEIZI CO.,LTD.

Free format text: FORMER NAME OR ADDRESS: NOVO NORDISK BIOTECH INC.

CP01 Change in the name or title of a patent holder

Address after: American California

Patentee after: Novo Jymes A/S

Address before: American California

Patentee before: Novozymes Biotech Inc.

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20050223

Termination date: 20170520

CF01 Termination of patent right due to non-payment of annual fee