ATE395424T1 - Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen - Google Patents
Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzenInfo
- Publication number
- ATE395424T1 ATE395424T1 AT99925717T AT99925717T ATE395424T1 AT E395424 T1 ATE395424 T1 AT E395424T1 AT 99925717 T AT99925717 T AT 99925717T AT 99925717 T AT99925717 T AT 99925717T AT E395424 T1 ATE395424 T1 AT E395424T1
- Authority
- AT
- Austria
- Prior art keywords
- mutant
- nucleic acid
- relates
- trichothecene
- cells
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- LZAJKCZTKKKZNT-PMNGPLLRSA-N trichothecene Chemical compound C12([C@@]3(CC[C@H]2OC2C=C(CCC23C)C)C)CO1 LZAJKCZTKKKZNT-PMNGPLLRSA-N 0.000 title abstract 3
- 229930013292 trichothecene Natural products 0.000 title abstract 3
- 241000233866 Fungi Species 0.000 title 1
- 230000007812 deficiency Effects 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 5
- 238000000034 method Methods 0.000 abstract 4
- 108700023225 Trichodiene synthases Proteins 0.000 abstract 3
- 230000002538 fungal effect Effects 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000012986 modification Methods 0.000 abstract 2
- 230000004048 modification Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
- C12Y402/03006—Trichodiene synthase (4.2.3.6)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8221798A | 1998-05-20 | 1998-05-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE395424T1 true ATE395424T1 (de) | 2008-05-15 |
Family
ID=22169802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99925717T ATE395424T1 (de) | 1998-05-20 | 1999-05-20 | Verfahren zur herstellung von heterologen polypeptiden in trichothecene mangelmutanten filamentlösen pilzen |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US6180366B1 (enExample) |
| EP (1) | EP1078081B1 (enExample) |
| JP (2) | JP2002515253A (enExample) |
| CN (2) | CN1190495C (enExample) |
| AT (1) | ATE395424T1 (enExample) |
| AU (1) | AU4195099A (enExample) |
| DE (1) | DE69938722D1 (enExample) |
| DK (1) | DK1078081T3 (enExample) |
| WO (1) | WO1999060137A2 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6060305A (en) * | 1994-06-30 | 2000-05-09 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic Fusarium expression system |
| ATE466603T1 (de) * | 1998-04-27 | 2010-05-15 | Surmodics Inc | Bioaktive wirkstoffe freisetzende beschichtungen |
| AU4195099A (en) * | 1998-05-20 | 1999-12-06 | Novo Nordisk Biotech, Inc. | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells |
| FR2826978B1 (fr) * | 2001-07-06 | 2004-09-17 | Agronomique Inst Nat Rech | Procede de determination de la capacite de fusarium a produire des toxines trichotecenes |
| US8148155B2 (en) * | 2002-04-22 | 2012-04-03 | Novozymes, Inc. | Methods for increasing homologous recombination of a nucleic acid sequence |
| DK1546302T3 (da) * | 2002-08-30 | 2009-12-14 | Novozymes Inc | Fremgangsmåder til fremstilling af pattedyrs-trypsiner |
| AU2003258395A1 (en) * | 2002-08-30 | 2004-03-19 | Puratos Naamloze Vennootschap | Myrothecium sp. transformation and expression system |
| JP4490284B2 (ja) | 2002-11-18 | 2010-06-23 | ノボザイムス，インコーポレイティド | 菌類細胞において遺伝子を発現するためのプロモーター変異体 |
| US20090233287A1 (en) * | 2006-04-25 | 2009-09-17 | Roelof Ary Lans Bovenberg | Production of compounds in a recombinant host |
| RU2008149928A (ru) * | 2006-05-18 | 2010-06-27 | Конинклейке Филипс Электроникс Н.В. (Nl) | Горизонтальное тостерное устройство с относительно малыми верхними нагревательными средствами |
| CN102227502B (zh) | 2008-09-30 | 2016-01-13 | 诺维信股份有限公司 | 在镶片镰孢的酶缺陷突变体中产生多肽的方法 |
| US20100316764A1 (en) * | 2009-06-10 | 2010-12-16 | Engrain, LLC | Flour supplement compositions and methods for preparing wheat flour |
| US20120184008A1 (en) * | 2009-08-05 | 2012-07-19 | Novozymes A/S | Filamentous fungi and methods for producing trichodiene from lignocellulosic feedstocks |
| BR112012013205B1 (pt) | 2009-12-18 | 2020-11-03 | Novozymes, Inc. | mutante de uma cepa parental de trichoderma reesei, e, métodos para produzir um polipeptídeo de interesse e para obter um mutante de uma cepa parental de trichoderma |
| CN108588060B (zh) * | 2017-03-07 | 2020-12-01 | 武汉康复得生物科技股份有限公司 | 一种用丝状真菌宿主细胞表达的重组草酸脱羧酶 |
| JP2021078354A (ja) | 2018-03-08 | 2021-05-27 | 有限会社アルティザイム・インターナショナル | フラビンアデニンジヌクレオチドグルコース脱水素酵素とシトクロム分子との融合タンパク質 |
| CN112522231B (zh) * | 2020-12-14 | 2023-03-14 | 广东省微生物研究所（广东省微生物分析检测中心） | 一种酰基转移酶及其编码基因和应用 |
| WO2023088791A1 (en) | 2021-11-22 | 2023-05-25 | Basf Se | Paenibacillus strains producing low amounts of dab comprising compounds. |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8904762D0 (en) * | 1989-03-02 | 1989-04-12 | Glaxo Group Ltd | Biological process |
| WO1996000787A1 (en) * | 1994-06-30 | 1996-01-11 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| US5594119A (en) * | 1994-09-20 | 1997-01-14 | Novo Nordisk A/S | Gene encoding carboxypeptidase of aspergillus niger |
| EP1975237B1 (en) * | 1996-03-11 | 2012-12-26 | Meiji Co., Ltd. | Beta-fructofuranosidase variants and uses thereof |
| AU4195099A (en) * | 1998-05-20 | 1999-12-06 | Novo Nordisk Biotech, Inc. | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells |
| US6383781B1 (en) * | 1998-12-23 | 2002-05-07 | Novozymes A/S | Methods for producing polypeptides in aspergillus mutant cells |
-
1999
- 1999-05-20 AU AU41950/99A patent/AU4195099A/en not_active Abandoned
- 1999-05-20 DK DK99925717T patent/DK1078081T3/da active
- 1999-05-20 WO PCT/US1999/011223 patent/WO1999060137A2/en not_active Ceased
- 1999-05-20 US US09/316,080 patent/US6180366B1/en not_active Expired - Lifetime
- 1999-05-20 AT AT99925717T patent/ATE395424T1/de not_active IP Right Cessation
- 1999-05-20 CN CNB998081124A patent/CN1190495C/zh not_active Expired - Fee Related
- 1999-05-20 DE DE69938722T patent/DE69938722D1/de not_active Expired - Lifetime
- 1999-05-20 EP EP99925717A patent/EP1078081B1/en not_active Expired - Lifetime
- 1999-05-20 JP JP2000549743A patent/JP2002515253A/ja active Pending
- 1999-05-20 CN CNB031588026A patent/CN1231579C/zh not_active Expired - Fee Related
-
2005
- 2005-03-31 US US11/095,491 patent/US7332341B2/en not_active Expired - Fee Related
-
2008
- 2008-01-31 US US12/023,417 patent/US20080213901A1/en not_active Abandoned
-
2009
- 2009-08-19 JP JP2009190191A patent/JP2009291207A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN1190495C (zh) | 2005-02-23 |
| CN1231579C (zh) | 2005-12-14 |
| WO1999060137A2 (en) | 1999-11-25 |
| DK1078081T3 (da) | 2008-08-04 |
| WO1999060137A3 (en) | 2000-05-18 |
| US20050181480A1 (en) | 2005-08-18 |
| EP1078081B1 (en) | 2008-05-14 |
| US20080213901A1 (en) | 2008-09-04 |
| DE69938722D1 (de) | 2008-06-26 |
| JP2002515253A (ja) | 2002-05-28 |
| JP2009291207A (ja) | 2009-12-17 |
| CN1309711A (zh) | 2001-08-22 |
| US7332341B2 (en) | 2008-02-19 |
| CN1500882A (zh) | 2004-06-02 |
| US6180366B1 (en) | 2001-01-30 |
| AU4195099A (en) | 1999-12-06 |
| EP1078081A2 (en) | 2001-02-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |