CN118121664A - Antibacterial mite-killing composition, preparation method thereof and eye mask - Google Patents
Antibacterial mite-killing composition, preparation method thereof and eye mask Download PDFInfo
- Publication number
- CN118121664A CN118121664A CN202410151662.2A CN202410151662A CN118121664A CN 118121664 A CN118121664 A CN 118121664A CN 202410151662 A CN202410151662 A CN 202410151662A CN 118121664 A CN118121664 A CN 118121664A
- Authority
- CN
- China
- Prior art keywords
- parts
- mite
- antibacterial
- composition
- killing composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 69
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 53
- 238000002360 preparation method Methods 0.000 title abstract description 13
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 24
- 240000000011 Artemisia annua Species 0.000 claims abstract description 19
- 235000001405 Artemisia annua Nutrition 0.000 claims abstract description 19
- 244000179886 Moringa oleifera Species 0.000 claims abstract description 18
- 235000011347 Moringa oleifera Nutrition 0.000 claims abstract description 18
- 244000284012 Vetiveria zizanioides Species 0.000 claims abstract description 18
- 235000007769 Vetiveria zizanioides Nutrition 0.000 claims abstract description 18
- 240000001899 Murraya exotica Species 0.000 claims abstract description 17
- 235000008766 Murraya exotica Nutrition 0.000 claims abstract description 17
- 235000009696 Murraya paniculata Nutrition 0.000 claims abstract description 17
- 241000244938 Penthorum chinense Species 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 241000233779 Cyclocarya paliurus Species 0.000 claims abstract description 5
- 241000218652 Larix Species 0.000 claims abstract description 3
- 235000005590 Larix decidua Nutrition 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 29
- 239000000243 solution Substances 0.000 claims description 20
- 230000000895 acaricidal effect Effects 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 238000002137 ultrasound extraction Methods 0.000 claims description 7
- 241000632227 Antenoron Species 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 5
- 230000002829 reductive effect Effects 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- 238000004042 decolorization Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 13
- 241000238876 Acari Species 0.000 abstract description 10
- 241000894006 Bacteria Species 0.000 abstract description 7
- 230000002195 synergetic effect Effects 0.000 abstract description 5
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 208000003464 asthenopia Diseases 0.000 abstract description 4
- 208000030533 eye disease Diseases 0.000 abstract description 4
- 230000007794 irritation Effects 0.000 abstract description 4
- 230000002740 effect on eyes Effects 0.000 abstract description 3
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 40
- 241001128004 Demodex Species 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 208000024891 symptom Diseases 0.000 description 7
- 208000003556 Dry Eye Syndromes Diseases 0.000 description 5
- 206010013774 Dry eye Diseases 0.000 description 5
- 230000006872 improvement Effects 0.000 description 5
- 230000002147 killing effect Effects 0.000 description 5
- 241000222122 Candida albicans Species 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 229940095731 candida albicans Drugs 0.000 description 4
- 210000003780 hair follicle Anatomy 0.000 description 4
- 230000003110 anti-inflammatory effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 244000000010 microbial pathogen Species 0.000 description 3
- 230000003071 parasitic effect Effects 0.000 description 3
- 210000001732 sebaceous gland Anatomy 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 239000000341 volatile oil Substances 0.000 description 3
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 2
- 208000003322 Coinfection Diseases 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 240000003553 Leptospermum scoparium Species 0.000 description 2
- 235000015459 Lycium barbarum Nutrition 0.000 description 2
- 206010034960 Photophobia Diseases 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 206010047513 Vision blurred Diseases 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- JMFRWRFFLBVWSI-NSCUHMNNSA-N coniferol Chemical compound COC1=CC(\C=C\CO)=CC=C1O JMFRWRFFLBVWSI-NSCUHMNNSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 210000000720 eyelash Anatomy 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 210000004175 meibomian gland Anatomy 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000035807 sensation Effects 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 description 1
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 1
- 240000006891 Artemisia vulgaris Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 235000008734 Bergera koenigii Nutrition 0.000 description 1
- 244000166675 Cymbopogon nardus Species 0.000 description 1
- 235000018791 Cymbopogon nardus Nutrition 0.000 description 1
- 241000193880 Demodex folliculorum Species 0.000 description 1
- 241000522190 Desmodium Species 0.000 description 1
- 206010013082 Discomfort Diseases 0.000 description 1
- 206010015958 Eye pain Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000334154 Isatis tinctoria Species 0.000 description 1
- 206010023644 Lacrimation increased Diseases 0.000 description 1
- 241001571764 Lysimachia christinae Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 240000002393 Murraya koenigii Species 0.000 description 1
- NEAPKZHDYMQZCB-UHFFFAOYSA-N N-[2-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]ethyl]-2-oxo-3H-1,3-benzoxazole-6-carboxamide Chemical compound C1CN(CCN1CCNC(=O)C2=CC3=C(C=C2)NC(=O)O3)C4=CN=C(N=C4)NC5CC6=CC=CC=C6C5 NEAPKZHDYMQZCB-UHFFFAOYSA-N 0.000 description 1
- 235000011615 Pinus koraiensis Nutrition 0.000 description 1
- 240000007263 Pinus koraiensis Species 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 240000000513 Santalum album Species 0.000 description 1
- 235000008632 Santalum album Nutrition 0.000 description 1
- 241000967218 Selaginella tamariscina Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940119526 coniferyl alcohol Drugs 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 229930004069 diterpene Natural products 0.000 description 1
- 125000000567 diterpene group Chemical group 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000000744 eyelid Anatomy 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 230000004317 lacrimation Effects 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- 229930007744 linalool Natural products 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- SFMJNHNUOVADRW-UHFFFAOYSA-N n-[5-[9-[4-(methanesulfonamido)phenyl]-2-oxobenzo[h][1,6]naphthyridin-1-yl]-2-methylphenyl]prop-2-enamide Chemical compound C1=C(NC(=O)C=C)C(C)=CC=C1N1C(=O)C=CC2=C1C1=CC(C=3C=CC(NS(C)(=O)=O)=CC=3)=CC=C1N=C2 SFMJNHNUOVADRW-UHFFFAOYSA-N 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- -1 polyphenol compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229930004725 sesquiterpene Natural products 0.000 description 1
- 150000004354 sesquiterpene derivatives Chemical class 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004488 tear evaporation Effects 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 229940126673 western medicines Drugs 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of daily necessities, in particular to a bacteriostatic mite-killing composition, a preparation method thereof and eye patches. The antibacterial and mite-killing composition is prepared from the following raw materials in parts by weight: 10-30 parts of artemisia annua, 10-30 parts of murraya paniculata, 10-20 parts of vetiver, 10-20 parts of cyclocarya paliurus, 5-15 parts of moringa oleifera leaves and 5-15 parts of penthorum chinense pursh. The antibacterial and mite-killing composition has the advantages that the proportion among the artemisia annua, the murraya paniculata, the vetiver, the larch, the moringa oleifera leaf and the penthorum chinense pursh is limited within a reasonable range, so that a better synergistic effect is exerted among the components, the antibacterial and mite-killing composition has a better antibacterial and mite-killing function, and after the antibacterial and mite-killing composition is applied to eye patches, the eye fatigue can be relieved, and eye diseases caused by mites and bacteria can be prevented. In addition, the raw materials of the antibacterial and mite-killing composition disclosed by the invention are plant components, so that the antibacterial and mite-killing composition has no toxic or side effect and can not cause irritation or side effect on eyes.
Description
Technical Field
The invention relates to the technical field of daily necessities, in particular to a bacteriostatic mite-killing composition, a preparation method thereof and eye patches.
Background
Demodex is a permanent small parasitic mite which mainly hosts in hair follicles and sebaceous glands of human and mammals, and currently known species are hundreds of species, and only two species are found which can parasitic on the body surface of a human body, namely the hair follicle Demodex and the sebaceous Demodex. Demodex belongs to a conditionally pathogenic parasite, a small amount of parasitic body surfaces of Demodex does not usually cause diseases, and when the number of Demodex population is increased or the immunity of a human body is reduced, a disease state can occur. In a study involving 335 patients with ocular surface discomfort, it was found that 84% of patients had demodex mites in their eyes and that the number of demodex mites was significantly positively correlated with subjective ocular surface discomfort.
In the eye, demodex folliculorum mainly lodges in the hair follicles of the eyelashes, while Demodex sebaceous mites submerge deep in sebaceous and meibomian glands. Demodex mites can be used directly as carriers of pathogenic microorganisms, and when the insects enter hair follicles and sebaceous glands, the insects carry certain pathogenic microorganisms into meibomian glands to cause secondary infection, and the secondary infection comprises staphylococcus and streptococcus. These pathogenic microorganisms enter the eye and alter the lipid tear film, causing excessive tear evaporation and reduced tear film stability, ultimately leading to the onset of dry eye. In 2018, the chinese minute of asian dry eye association reached expert consensus that ocular demodex infestations caused dry eye, and clinical symptoms of the infection were reddish on eyelid, itching eye, dry eye, burning eye, foreign body sensation, photophobia and increased secretion, and serious patients were accompanied by repeated eyelash drop, blurred vision or vision drop.
In the prior art, most plant components are tea tree essential oil, but the essential oil components have certain irritation, anaphylactic reaction occurs to a small number of people, and a safe and effective component for removing mites and inhibiting bacteria of eyes is necessary.
Disclosure of Invention
In order to solve the technical problems, the invention provides the antibacterial mite-removing composition, the preparation method thereof and the eye mask, and the antibacterial mite-removing composition is applied to the eye mask, so that after the application, the eye fatigue can be relieved, and eye diseases caused by mites and bacteria can be prevented.
According to a first aspect of the invention, the invention provides a bacteriostatic and anti-mite composition, which is prepared from the following raw materials in parts by weight: 10-30 parts of artemisia annua, 10-30 parts of murraya paniculata, 10-20 parts of vetiver, 10-20 parts of cyclocarya paliurus, 5-15 parts of moringa oleifera leaves and 5-15 parts of penthorum chinense pursh.
In the scheme, the antibacterial and mite-removing composition comprises artemisia annua, murraya paniculata, vetiver, lysimachia christinae, moringa oleifera leaves and penthorum chinense pursh, wherein the artemisia annua is rich in polyphenol compounds, coniferyl alcohol and quercetin, and the artemisia annua has antibacterial and anti-inflammatory effects. The main components of the Murraya paniculata include tea tree alcohol, linalool, etc., and have strong antibacterial effect. The main components of the vetiver are saponin, flavone, polysaccharide and the like, and the components have strong antibacterial and anti-inflammatory effects. The herba Desmodii Styracifolii contains various chemical components such as diterpenes, triterpenes, sesquiterpenes, lignans and flavonoids, and has antibacterial effect. The moringa leaves contain various active ingredients such as flavonoids, phenolic acids and the like, can inhibit inflammatory reaction and bacterial infection, and has certain effects on preventing and treating inflammatory diseases. The penthorum chinense pursh contains abundant volatile oil and various active ingredients, has remarkable antibacterial and anti-inflammatory effects, and can inhibit the growth of bacteria and fungi. According to the antibacterial and mite-killing composition, the proportion of the artemisia annua, the murraya paniculata, the vetiver, the longhairy antenoron herb, the moringa oleifera leaf and the penthorum chinense pursh is limited within a reasonable range, so that a better synergistic effect is exerted among the components, the antibacterial and mite-killing composition has a better antibacterial and mite-killing function, and after the antibacterial and mite-killing composition is applied to eye patches, the eye fatigue can be relieved, and eye diseases caused by mites and bacteria can be prevented. In addition, the raw materials of the antibacterial and mite-killing composition disclosed by the invention are plant components, so that the antibacterial and mite-killing composition has no toxic or side effect and can not cause irritation or side effect on eyes.
Further, in order to improve the interaction effect among the components, the antibacterial and mite-killing composition is prepared from the following raw materials in parts by weight: 15-25 parts of artemisia annua, 15-25 parts of murraya paniculata, 12-18 parts of vetiver, 12-18 parts of cyclocarya paliurus, 8-12 parts of moringa oleifera leaves and 8-12 parts of penthorum chinense pursh.
Further, the antibacterial and mite-killing composition is prepared from the following raw materials in parts by weight: 20 parts of artemisia annua, 20 parts of murraya paniculata, 15 parts of vetiver, 15 parts of larch, 10 parts of moringa oleifera leaves and 10 parts of penthorum chinense pursh.
According to a second aspect of the present invention, the present invention also provides a method for preparing the above bacteriostatic and anti-mite composition, comprising the steps of:
weighing artemisia annua, murraya paniculata, vetiver grass, longhairy antenoron herb, moringa leaf and penthorum chinense pursh, mixing, adding into ethanol solution for ultrasonic extraction, and filtering; concentrating the filtered filtrate, purifying, decolorizing, and drying to obtain antibacterial and mite-killing composition. Preferably, spray drying is used for drying.
In the scheme, the preparation method of the antibacterial and mite-killing composition comprises the steps of firstly carrying out ultrasonic extraction on the artemisia annua, the murraya paniculata, the vetiver, the longhairy antenoron herb, the moringa oleifera leaf and the penthorum chinense pursh by adopting ethanol solution to effectively extract active ingredients in all raw materials, then filtering, concentrating the filtered filtrate, purifying, further enriching the active ingredients, and decoloring to ensure that the color of the obtained final antibacterial and mite-killing composition is light, so that the color of the eye mask is not influenced, and the using impression comfort is improved.
Further, the ultrasonic extraction is to add 10-20 times of ethanol solution, and extract for 0.5-1.5h under the power of 20-40 KHz; preferably, the volume concentration of the ethanol solution is 40-60%.
In the method, the purpose of efficiently extracting the functional components in the raw materials is achieved by reasonably limiting the use amount of the extracting solution, the ultrasonic power and the time in the ultrasonic extraction process.
Further, the purification is to concentrate the filtered filtrate and then put the filtrate on an AB-8 macroporous resin column, firstly, the filtrate is eluted by distilled water with the volume of 2 to 4 times of the column volume, then, the filtrate is eluted by 70 percent ethanol with the volume of 2 to 4 times of the column volume, and the ethanol eluent is collected and concentrated under reduced pressure.
In the scheme, the purification efficiency can be improved, more effective components can be enriched, and the functions of the antibacterial and mite-killing composition can be improved by reasonably designing the purification process.
Further, the decolorization is to add the purified concentrated solution into active carbon, keep the temperature at 50-70 ℃ for 20-40min for decolorization, and filter, and take the filtrate.
In the scheme, the decoloring technology is reasonably designed, so that the color of the obtained antibacterial and mite-killing composition is lighter, and the use comfort level is improved.
Further, the addition amount of the activated carbon is 0.5 to 1.5% by mass, preferably 1% by mass of the purified concentrate.
In the scheme, the decoloring effect can be further improved by reasonably limiting the addition amount of the activated carbon in the decoloring process.
According to a third aspect of the present invention there is also provided an eye patch comprising a nonwoven and an aqueous solution comprising the bacteriostatic and anti-mite composition as described above.
Further, in the aqueous solution, the weight percentage of the antibacterial and acaricidal composition is 0.5-2%, preferably 1%.
The eye mask can be prepared by the following method: cutting non-woven fabric into proper shape, adding water solution containing the antibacterial and mite-killing composition, packaging, and performing radiation sterilization. The antibacterial and mite-killing composition has good mite-killing effect, can have the effect of killing mites at very low concentration,
The technical scheme provided by the invention has the following beneficial effects:
According to the antibacterial and mite-killing composition, the proportion of the artemisia annua, the murraya paniculata, the vetiver, the longhairy antenoron herb, the moringa oleifera leaf and the penthorum chinense pursh is limited within a reasonable range, so that a better synergistic effect is exerted among the components, the antibacterial and mite-killing composition has a better antibacterial and mite-killing function, and after the antibacterial and mite-killing composition is applied to eye patches, the eye fatigue can be relieved, and eye diseases caused by mites and bacteria can be prevented. In addition, the raw materials of the antibacterial and mite-killing composition disclosed by the invention are plant components, so that the antibacterial and mite-killing composition has no toxic or side effect and can not cause irritation or side effect on eyes.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples 1 to 5, comparative examples 1 to 2
Examples 1-5 and comparative examples 1-2 each independently provide an antibacterial and acaricidal composition, which comprises the following raw materials in parts by weight as shown in table 1, and the preparation method of the antibacterial and acarus-killing composition is as follows: weighing artemisia annua, murraya paniculata, vetiver grass, longhairy antenoron herb, moringa leaf and penthorum chinense pursh, mixing, adding into a multifunctional ultrasonic extraction tank, adding an ethanol solution with the concentration of 15 times of 50 percent, extracting for 1h at the power of 30KHz, filtering after the extraction, concentrating the filtrate, loading on an AB-8 macroporous resin column, eluting with distilled water with the volume of 3 times of the column, eluting with 70 percent ethanol with the volume of 3 times of the column, collecting ethanol eluent, concentrating under reduced pressure, adding 1 percent of active carbon into the concentrated solution, decolorizing at the temperature of 60 ℃, filtering, and spray-drying the filtrate to obtain antibacterial mite-removing composition powder.
TABLE 1
| Artemisia annua L | Murraya paniculata (L.) Gaertn | Vetiver grass | Herba Desmodii Styracifolii | Moringa oleifera leaf | Herba Penthori chinensis | |
| Example 1 | 20 | 20 | 15 | 15 | 10 | 10 |
| Example 2 | 10 | 10 | 10 | 10 | 5 | 5 |
| Example 3 | 30 | 30 | 20 | 20 | 15 | 15 |
| Example 4 | 15 | 15 | 12 | 12 | 8 | 8 |
| Example 5 | 25 | 25 | 18 | 18 | 12 | 12 |
| Comparative example 1 | 5 | 35 | 25 | 5 | 20 | 5 |
| Comparative example 2 | 35 | 5 | 5 | 25 | 5 | 20 |
Comparative example 3
This comparative example provides a bacteriostatic and acaricidal composition, which is different from example 1 in that the same amount of mugwort leaf is used instead of artemisia annua.
The preparation method of the bacteriostatic and acaricidal composition of the comparative example is the same as that of example 1.
Comparative example 4
This comparative example provides a bacteriostatic and acaricidal composition which differs from example 1 in that the same amount of citronella is used instead of Murraya koenigii.
The preparation method of the bacteriostatic and acaricidal composition of the comparative example is the same as that of example 1.
Comparative example 5
This comparative example provides a bacteriostatic and acaricidal composition, which differs from example 1 in that the same amount of sandalwood is used instead of vetiver.
The preparation method of the bacteriostatic and acaricidal composition of the comparative example is the same as that of example 1.
Comparative example 6
This comparative example provides a bacteriostatic and acaricidal composition which differs from example 1 in that the same amount of selaginella tamariscina is used instead of the pinus koraiensis.
The preparation method of the bacteriostatic and acaricidal composition of the comparative example is the same as that of example 1.
Comparative example 7
This comparative example provides a bacteriostatic and anti-mite composition, which differs from example 1 in that the same amount of dyers woad She Tidai moringa leaves are used.
The preparation method of the bacteriostatic and acaricidal composition of the comparative example is the same as that of example 1.
Comparative example 8
This comparative example provides a bacteriostatic mite-killing composition, which is different from example 1 in that the same amount of desmodium is used instead of penthorum chinense pursh.
The preparation method of the bacteriostatic and acaricidal composition of the comparative example is the same as that of example 1.
Experimental example
The anti-mite and anti-mite compositions prepared in the above examples and comparative examples were subjected to an anti-mite test and an anti-mite effect test, and specific test results are shown in tables 2 and 3 below:
1. Mite killing test: test method according to NY/T1151.2-2006, method for testing indoor efficacy of pesticides for pesticide registration sanitary purposes, and evaluation part 2: the mite killing test method in mite killing and dispelling agent. 1) Preparing the antibacterial and mite-killing composition into a solution with the content of 1%, taking 4 culture dishes, wherein 3 of the 4 culture dishes are sprayed with the antibacterial and mite-killing composition solution respectively by a spraying method, and the other culture dish is sprayed with distilled water as a blank control. Simultaneously, the upper edge of the inner wall of each culture dish is uniformly smeared with white oil and vaseline mixture. 2) About 200 experimental mites were placed in the center of each culture dish, 0.05g of mite feed was placed in the center of the culture dish after 30min, and the culture dish was placed in a water-proof incubator. 3) After 48 hours the number of dead mites was checked and recorded.
TABLE 2
| Test mite count (Only) | Number of dead mites (Only) | Mite killing rate% | |
| Example 1 | 603 | 603 | 100 |
| Example 2 | 598 | 582 | 97.3 |
| Example 3 | 601 | 580 | 96.5 |
| Example 4 | 587 | 580 | 98.8 |
| Example 5 | 594 | 583 | 98.1 |
| Comparative example 1 | 591 | 501 | 84.8 |
| Comparative example 2 | 610 | 499 | 81.8 |
| Comparative example 3 | 589 | 310 | 52.6 |
| Comparative example 4 | 605 | 297 | 49.1 |
| Comparative example 5 | 599 | 268 | 44.7 |
| Comparative example 6 | 595 | 275 | 46.2 |
| Comparative example 7 | 602 | 266 | 44.2 |
| Comparative example 8 | 601 | 210 | 34.9 |
| Blank group | 200 | 16 | 8 |
As shown in Table 2, the bacteriostatic and acaricidal composition provided by the invention has excellent acarid-removing effect, and the components in the bacteriostatic and acarid-removing composition have synergistic effect, and the lack of any component can greatly reduce the acarid-removing effect.
2. Antibacterial test
The test method is as follows:
Testing strains: staphylococcus aureus (ATCC 6538), escherichia coli (8099), candida albicans (ATCC 10231).
Preparing a bacterial suspension: the strain is taken for 24 hours, fresh slant culture is washed by 5ml of sterilized PBS, and the strain is diluted and prepared into bacterial suspension with the bacterial content of 10 4-105 cfu/ml for standby.
Bacteriostasis experiment: 4.5ml of 1% antibacterial and acaricidal composition solution and 0.5ml of bacterial suspension are added into a sterile test tube, and the mixture is uniformly mixed and acted for 10min. 1ml of the sample solution in the test tube is respectively inoculated into 2 sterile plates, poured into a nutrient agar medium or a Sahnikovia agar medium (candida albicans) cooled to 45 ℃ after melting, evenly mixed, and cultured for 48 hours (bacteria) or 72 hours (candida albicans) in a 37 ℃ incubator after cooling, and counted. The same treatment was performed using sterile distilled water instead of the liquid medicine as a positive control. All experiments were repeated three times, and the average bacteriostasis rate was calculated as: antibacterial ratio (%) = (average colony count of control-average colony count of test liquid)/average colony count of control x 100%.
TABLE 3 Table 3
As shown in Table 3, the antibacterial and mite-killing composition has excellent inhibition effects on staphylococcus aureus, escherichia coli and candida albicans, and the components in the antibacterial and mite-killing composition have synergistic effects, and the lack of any one of the components can greatly weaken the antibacterial effect.
3. Clinical study experiment
260 Persons using computers, mobile phones and other electronic products for a long time are clinically selected as study objects, and the study objects are divided into eye patches prepared by using the antibacterial and mite-removing compositions of examples 1-5 and comparative examples 1-8, wherein each group of 20 persons adopts a front-back comparison method, each person uses the eye patch for 3 times every day, applies the eye patch to eyes for 10 minutes every time, and continuously uses the eye patch for 30 days, and other health care products, traditional Chinese medicines and western medicines which influence the effect of observing and researching the eye patches are forbidden to use during the study.
Symptom improvement effectiveness: the 8 symptoms of eye ache, eye distension, photophobia, blurred vision, dry eyes, foreign body sensation, lacrimation and general discomfort are improved by 3 kinds, and the improvement of the symptoms is judged without deterioration of other symptoms. The symptom improvement effectiveness (%) was calculated as improvement cases/total cases×100. The symptom improvement effectiveness is shown in table 4.
TABLE 4 Table 4
| The effective number of people | Invalid number of people | Effective rate is% | |
| Example 1 | 19 | 1 | 95 |
| Example 2 | 18 | 2 | 90 |
| Example 3 | 17 | 3 | 85 |
| Example 4 | 18 | 2 | 90 |
| Example 5 | 17 | 3 | 85 |
| Comparative example 1 | 14 | 6 | 70 |
| Comparative example 2 | 10 | 10 | 50 |
| Comparative example 3 | 11 | 9 | 55 |
| Comparative example 4 | 8 | 12 | 40 |
| Comparative example 5 | 7 | 13 | 35 |
| Comparative example 6 | 8 | 12 | 40 |
| Comparative example 7 | 7 | 13 | 35 |
| Comparative example 8 | 7 | 13 | 35 |
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The antibacterial mite-killing composition is characterized by being prepared from the following raw materials in parts by weight: 10-30 parts of artemisia annua, 10-30 parts of murraya paniculata, 10-20 parts of vetiver, 10-20 parts of cyclocarya paliurus, 5-15 parts of moringa oleifera leaves and 5-15 parts of penthorum chinense pursh.
2. The bacteriostatic and anti-mite composition according to claim 1, which is prepared from the following raw materials in parts by weight: 15-25 parts of artemisia annua, 15-25 parts of murraya paniculata, 12-18 parts of vetiver, 12-18 parts of cyclocarya paliurus, 8-12 parts of moringa oleifera leaves and 8-12 parts of penthorum chinense pursh.
3. The bacteriostatic and acaricidal composition according to claim 2, which is characterized by being prepared from the following raw materials in parts by weight: 20 parts of artemisia annua, 20 parts of murraya paniculata, 15 parts of vetiver, 15 parts of larch, 10 parts of moringa oleifera leaves and 10 parts of penthorum chinense pursh.
4. A method of preparing a bacteriostatic and anti-mite composition according to any one of claims 1-3, comprising the steps of:
Weighing artemisia annua, murraya paniculata, vetiver grass, longhairy antenoron herb, moringa leaf and penthorum chinense pursh, mixing, adding into ethanol solution for ultrasonic extraction, and filtering; concentrating the filtered filtrate, purifying, decolorizing, and drying to obtain antibacterial and mite-killing composition.
5. The method according to claim 4, wherein the ultrasonic extraction is performed by adding 10-20 times of ethanol solution, and extracting for 0.5-1.5h at a power of 20-40 KHz; preferably, the volume concentration of the ethanol solution is 40-60%.
6. The method according to claim 4, wherein the purification is carried out by concentrating the filtered filtrate, loading on AB-8 macroporous resin column, eluting with 2-4 times of distilled water, eluting with 2-4 times of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure.
7. The method according to claim 4, wherein the decolorization is carried out by adding the purified concentrated solution into activated carbon, maintaining at 50-70deg.C for 20-40min, decolorizing, and filtering to obtain filtrate.
8. The method according to claim 7, wherein the activated carbon is added in an amount of 0.5-1.5%, preferably 1% of the mass of the purified concentrate.
9. An eye patch comprising a nonwoven and an aqueous solution comprising the bacteriostatic and anti-mite composition of any one of claims 1-3.
10. An eye patch according to claim 9, wherein the weight percentage of the bacteriostatic and anti-mite composition in the aqueous solution is 0.5-2%, preferably 1%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410151662.2A CN118121664A (en) | 2024-02-02 | 2024-02-02 | Antibacterial mite-killing composition, preparation method thereof and eye mask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410151662.2A CN118121664A (en) | 2024-02-02 | 2024-02-02 | Antibacterial mite-killing composition, preparation method thereof and eye mask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118121664A true CN118121664A (en) | 2024-06-04 |
Family
ID=91236811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410151662.2A Pending CN118121664A (en) | 2024-02-02 | 2024-02-02 | Antibacterial mite-killing composition, preparation method thereof and eye mask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118121664A (en) |
-
2024
- 2024-02-02 CN CN202410151662.2A patent/CN118121664A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101532005B1 (en) | Natural preservative comprising citrus junos seed extract and manufacturing method thereof | |
| WO2006109898A1 (en) | Antimicrobial composition containing natural extract, nano silver and natural essential oil | |
| CN110732014B (en) | Traditional Chinese medicine composition for removing mites as well as preparation method and application thereof | |
| CN113081928A (en) | Plant bacteriostatic gel and preparation method and application thereof | |
| CN102552504A (en) | Externally applied disinfectant for treating vaginitis and preparation method thereof | |
| CN110179693B (en) | Mosquito-repelling itching-relieving compound essential oil, preparation method and application thereof, and mosquito-repelling itching-relieving article | |
| CN110327259B (en) | Wet tissue for children | |
| CN112999128B (en) | Natural galenical composition with mosquito repelling and itching relieving functions | |
| CN114532368A (en) | Aromatic essential oil air disinfectant and preparation method thereof | |
| CN104490679B (en) | A kind of composite bactericidal hand cleanser and preparation method thereof | |
| CN108079165A (en) | A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt and preparation method thereof | |
| CN105012581A (en) | Fresh aloe antibacterial composite, application and preparing method of fresh aloe antibacterial composite | |
| CN105708763B (en) | A kind of floral water containing Folium Artemisiae Argyi extract and preparation method thereof | |
| CN110251580B (en) | Composite antibacterial agent and preparation method and application thereof | |
| CN106390182A (en) | Sanitary napkin with antibacterial natural fiber surface layer and manufacturing method of sanitary napkin with antibacterial natural fiber surface layer | |
| CN118121664A (en) | Antibacterial mite-killing composition, preparation method thereof and eye mask | |
| CN111481567B (en) | Compound ozone oil and preparation method and application thereof | |
| US4584198A (en) | Therapeutic compositions having antibacteric activity comprising a fraction extracted from camomile flowers and process for the preparation of said fraction | |
| CN1202233C (en) | Compound lotion detergent for cleaning and sterilizing and its preparing method | |
| CN111035684A (en) | Ozone oil compound rhinitis gel and preparation method thereof | |
| KR20220052702A (en) | Manufacturing method of antimicrobial composition containing natural plant extract and antimicrobial composition thereof | |
| CN113456549B (en) | Mosquito repellent composition and toilet water containing same | |
| KR101432996B1 (en) | CLEANSING COMPOSITION COMPRISING NATURAL COMPOUNDS EXTRACT OF Oenanthe javanica DC, Artemisia capillaris and Artemisiae capillaris Flos COMPLEX FOR LADIES, ITS METHOD TO USE AND PROCESS THEREOF | |
| CN111419905A (en) | Traditional Chinese medicine insect expelling composition and preparation method thereof | |
| CN111296508A (en) | Disinfectant for indoor air disinfection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |