CN112999128B

CN112999128B - Natural galenical composition with mosquito repelling and itching relieving functions

Info

Publication number: CN112999128B
Application number: CN202110293499.XA
Authority: CN
Inventors: 不公告发明人
Original assignee: Shaanxi Beikangjian Biotechnology Co ltd
Current assignee: Shaanxi Beikangjian Biotechnology Co ltd
Priority date: 2021-03-19
Filing date: 2021-03-19
Publication date: 2022-07-08
Anticipated expiration: 2041-03-19
Also published as: CN112999128A

Abstract

The invention relates to the technical field of daily chemical industry, and in particular relates to a mosquito repellent composition, a preparation method and application thereof. The mosquito repellent composition comprises the following raw materials in parts by weight: 0.01-1.0 part of magnolia bark extract, 0.01-1.0 part of blumea balsamifera extract, 0.01-2.0 parts of common cnidium fruit extract, 0.02-2.0 parts of mint extract, 0.1-2.0 parts of orange peel extract, 0.5-1.5 parts of pepper extract and 0.2-1.0 part of ethanol. The preparation method comprises the following steps: respectively adding water into cortex Magnolia officinalis, herba Blumeae Balsamiferae, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, distilling with water vapor to extract volatile oil, filtering, concentrating to obtain concentrated solution, and mixing the volatile oil and concentrated solution to obtain extracts; then adding 10-25% acetone water solution into fructus Cnidii, extracting, filtering, and concentrating to obtain fructus Cnidii extract; and finally, uniformly mixing the extracts and ethanol to obtain the composition. The composition has effects of repelling mosquito for a long time, relieving itching, diminishing inflammation, and killing bacteria.

Description

Natural galenical composition with mosquito repelling and itching relieving functions

Technical Field

The invention relates to the technical field of daily chemical industry, in particular to a natural galenical composition with mosquito repelling and itching relieving functions, a preparation method and application thereof.

Background

The female mosquito can survive, lay eggs and propagate offspring only by sucking blood, the smell sense of the female mosquito is extremely sensitive, and the female mosquito can directly wash a blood sucking object from 30m away. Mosquitoes often carry pathogenic bacteria such as staphylococcus aureus, bacillus subtilis, escherichia coli, pseudomonas aeruginosa and the like, skin is stabbed and itchy after being bitten by the mosquitoes, skin is easy to chap and infect after being repeatedly scratched, a long-term scar can be caused due to improper treatment, diseases are easily spread, and learning, working and life of people are influenced.

Mosquito-repellent and itching-relieving products in the market are full of Lin and Lang, such as toilet water, lithospermum paste, essential oil and the like, most of the mosquito-repellent toilet water contains a chemosynthetic repellent-DEET (DEET) or mosquito-repellent ester, and traditional mosquito-repellent incense and electrothermal mosquito-repellent incense sheets or electrothermal mosquito-repellent incense liquid which are popular in recent years contain pyrethroid chemical insecticides.

The chemosynthesis repellent has certain irritation and toxicity, and relatively safe botanical mosquito-repellent itching-relieving products have wide market prospect along with the improvement of the safety consciousness of people and the increasingly intense market competition. Compared with chemical insecticides, the plant essential oil has the characteristics of good advantages in the aspect of safety, such as fresh smell, no burning sensation, skin irritation, low toxicity or no toxicity, no drug effect residue after use, easy degradation, no environmental pollution and the like, but the natural repellent has low efficiency and durability in repelling mosquitoes.

Therefore, the development of a natural plant preparation composition with lasting excellent mosquito-repelling, itching-relieving, sterilizing and disinfecting effects is urgently needed.

Chinese patent application CN110638700A discloses a mosquito repellent liquid extracted from pure plants and a preparation method thereof. The mosquito repellent liquid extracted from pure plants is prepared from the following raw materials, by volume, 2-4 parts of a taxus chinensis leaching solution, 3-4 parts of a artemisia apiacea leaching solution, 1-2 parts of a sophora flavescens leaching solution, 1-2 parts of a fructus cnidii leaching solution and 87 parts of deionized water. The preparation method comprises the steps of primary extraction, purification and mixing. The mosquito repellent is prepared by mixing and processing pure plant extract, is suitable for infants, pregnant women and allergy-prone people, and needs to further improve the mosquito repellent rate.

Chinese invention patent application CN110755346A discloses an anophelifuge itching-relieving Coptis chinensis Franch toilet water, which comprises the following raw materials in parts by weight: 20-35 parts of coptis deltoidea, 10-20 parts of honeysuckle, 10-15 parts of lemon essential oil, 1-5 parts of fructus cnidii extract essential oil, 1-5 parts of rhizoma zedoariae, 1-5 parts of rhizoma corydalis, 1-5 parts of camphor, 1-5 parts of radix stemonae, 5-10 parts of salvia miltiorrhiza, 5-10 parts of astragalus membranaceus and 1-5 parts of edible alcohol. However, the floral water has more medicinal taste and more complex extraction process.

The Chinese patent application CN106726912A discloses a preparation method of an itching-relieving mosquito repellent liquid, which is prepared by the following steps: adding 5-10 parts by weight of hydroxyethyl piperidine carboxylic isobutyl ester into 25 parts by weight of ethanol aqueous solution to obtain a first solution, adding 10-15 parts by weight of fructus cnidii extract, 10-15 parts by weight of fructus kochiae and 10-20 parts by weight of folium artemisiae argyi into 120 parts by weight of ethanol aqueous solution, and carrying out reflux extraction at constant temperature of 30-40 ℃ and 0.15MPa for 4 hours to obtain a second solution; concentrating the second solution to 50-60 parts by weight and mixing with the first solution; adding pH regulator to adjust to 6.5. The invention not only contains plant raw materials, but also adds chemical mosquito repellent, and is not good for health after long-term use.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to solve the problem of providing a natural galenical composition which has simple raw materials, wide sources, long mosquito repelling time, itching relieving, inflammation diminishing and sterilization.

The invention aims to provide a natural galenical composition with mosquito repelling and itching relieving functions, which comprises the following raw materials: magnolia bark extract, blumea balsamifera extract, fructus cnidii extract, mint extract, citrus peel extract, pepper extract and ethanol.

Preferably, the raw materials of the composition comprise the following components in parts by weight: 0.01-1.0 part of magnolia bark extract, 0.01-1.0 part of blumea balsamifera extract, 0.01-2.0 parts of common cnidium fruit extract, 0.02-2.0 parts of mint extract, 0.1-2.0 parts of orange peel extract, 0.5-1.5 parts of pepper extract and 0.2-1.0 part of ethanol.

Preferably, the raw materials of the composition comprise the following components in parts by weight: 0.1-1.0 part of magnolia officinalis extract, 0.1-1.0 part of blumea balsamifera extract, 1-2 parts of fructus cnidii extract, 0.5-1 part of mint extract, 1-2 parts of citrus peel extract, 1.0-1.5 parts of pepper extract and 0.5-1.0 part of ethanol.

Preferably, the ethanol is 95% ethanol;

preferably, the preparation method of the torilidis fructus extract comprises the following steps: extracting fructus Cnidii with 10-25% acetone water solution, filtering, and concentrating to obtain fructus Cnidii extract.

Preferably, the extraction temperature in the preparation method of the common cnidium fruit is 40-50 ℃;

preferably, the extraction time is 2-4h, and the mass ratio of the 10-25% acetone aqueous solution to the fructus cnidii is 6-10: 1.

Another object of the present invention is to provide a method for preparing the above composition, comprising the following steps

(1) Pulverizing cortex Magnolia officinalis, herba Blumeae Balsamiferae, fructus Cnidii, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, and sieving;

(2) respectively adding water into cortex Magnolia officinalis, herba Blumeae Balsamiferae, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, distilling with water vapor to extract volatile oil, filtering, concentrating the filtrate to obtain concentrated solution, and mixing the volatile oil and concentrated solution to obtain cortex Magnolia officinalis extract, herba Blumeae Balsamiferae extract, fructus Cnidii extract, herba Menthae extract, pericarpium Citri Tangerinae extract and fructus Zanthoxyli extract;

(3) extracting fructus Cnidii with 10-25% acetone water solution, filtering, concentrating, and drying to obtain fructus Cnidii extract;

(4) mixing the above extracts with ethanol to obtain the composition.

Preferably, in the step (1), the crushed mesh number is 10-65 meshes.

Preferably, in the step (2), the extraction time is 5-7h, and the mass ratio of the water to the medicinal materials is 6-8: 1.

Preferably, in step (3), the extraction temperature is 40-50 ℃.

Preferably, in the step (3), the leaching time is 2-4h, and the mass ratio of the 10-25% acetone aqueous solution to the fructus cnidii is 6-10: 1.

Preferably, in the step (2), the concentration is carried out at 50-60 ℃ under reduced pressure until the relative density of the concentrated solution at 50-60 ℃ is 1.05-1.20.

The invention also aims to provide the application of the natural plant preparation composition in preparing mosquito repellent products.

The invention has the beneficial effects that:

(1) the mosquito repellent composition has simple formula raw materials, can repel mosquitoes, diminish inflammation, relieve itching and sterilize, and experimental researches show that the extract composition has long mosquito repelling time, can protect people from being invaded by mosquitoes for a long time in a damp and hot environment; the composition is a pure natural plant formula, and is safe and non-toxic compared with chemical mosquito repelling;

(2) according to the invention, the blumea balsamifera extract, the fructus cnidii extract and the magnolia officinalis extract are combined, so that the synergistic effect is good, and the mosquito repelling and itching relieving effects of the mosquito repelling composition are obviously improved.

(3) The invention also researches the preparation method of the fructus cnidii extract, and unexpectedly discovers that the fructus cnidii extract is prepared by extracting with a certain amount of acetone aqueous solution as a solvent, and the mosquito repellent effect of the composition is better than that of the extract prepared by water or ethanol with different concentrations.

Detailed Description

The technical solution of the present invention is further illustrated by the following examples.

Example 1

The natural galenical composition for repelling mosquitoes and relieving itching comprises the following raw materials:

0.1 part of magnolia officinalis extract, 1 part of blumea balsamifera extract, 2.0 parts of fructus cnidii extract, 0.5 part of mint extract, 1.0 part of citrus peel extract, 1.0 part of pepper extract, 0.5 part of 95% ethanol and a proper amount of water;

the preparation method of the mosquito repellent composition comprises the following steps:

(1) pulverizing cortex Magnolia officinalis, herba Blumeae Balsamiferae, fructus Cnidii, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, and sieving with 10 mesh sieve;

(2) respectively adding 6 times of water into cortex Magnolia officinalis, herba Blumeae Balsamiferae, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, steam distilling to extract volatile oil, extracting for 5 hr, filtering, concentrating the filtrate at 50 deg.C under reduced pressure to obtain concentrated solution with relative density of 1.08(50 deg.C), mixing volatile oil and concentrated solution to obtain cortex Magnolia officinalis extract, herba Blumeae Balsamiferae extract, fructus Cnidii extract, herba Menthae extract, pericarpium Citri Tangerinae extract and fructus Zanthoxyli extract;

(3) extracting fructus Cnidii with 8 times of 10% acetone water solution at 40 deg.C for 3 hr, filtering, concentrating, and drying to obtain fructus Cnidii extract

(4) Mixing the above extracts with 95% ethanol to obtain composition;

(5) and adding water to 100 parts of the composition to obtain the mosquito repellent liquid.

Example 2

1.0 part of magnolia officinalis extract, 0.1 part of blumea balsamifera extract, 1.0 part of fructus cnidii extract, 1.0 part of mint extract, 2 parts of citrus peel extract, 1.5 parts of pepper extract, 1.0 part of 95% ethanol and a proper amount of water;

(1) pulverizing cortex Magnolia officinalis, herba Blumeae Balsamiferae, fructus Cnidii, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, and sieving with 65 mesh sieve;

(2) respectively adding 8 times of water into medicinal materials such as mangnolia officinalis, blumea balsamifera, mint, orange peel and pepper, extracting volatile oil by steam distillation, extracting for 7h, filtering, concentrating the filtrate at 60 ℃ under reduced pressure to obtain concentrated solution with the relative density of 1.20(50 ℃), and mixing the volatile oil and the concentrated solution to obtain a mangnolia officinalis extract, a blumea balsamifera extract, a mint extract, an orange peel extract and a pepper extract;

(3) adding 6 times of 25% acetone water solution into fructus Cnidii, extracting at 50 deg.C for 4 hr, filtering, concentrating, and drying to obtain fructus Cnidii extract;

(4) mixing the above extracts with 95% ethanol to obtain composition;

Example 3

1.0 part of magnolia officinalis extract, 1.0 part of blumea balsamifera extract, 1.0 part of fructus cnidii extract, 1.0 part of mint extract, 1.0 part of citrus peel extract, 1.5 parts of pepper extract, 0.5 part of 95% ethanol and a proper amount of water.

(1) pulverizing cortex Magnolia officinalis, herba Blumeae Balsamiferae, fructus Cnidii, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, and sieving with 24 mesh sieve;

(2) respectively adding 8 times of water into cortex Magnolia officinalis, herba Blumeae Balsamiferae, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, steam distilling to extract volatile oil, extracting for 6 hr, filtering, concentrating filtrate at 60 deg.C under reduced pressure to obtain concentrated solution with relative density of 1.20(50 deg.C), mixing volatile oil and concentrated solution to obtain cortex Magnolia officinalis extract, herba Blumeae Balsamiferae extract, herba Menthae extract, pericarpium Citri Tangerinae extract and fructus Zanthoxyli extract;

(3) adding 10 times of 10% acetone water solution into fructus Cnidii, extracting at 55 deg.C for 2 hr, filtering, concentrating, and drying to obtain fructus Cnidii extract;

(4) mixing the above extracts with 95% ethanol to obtain composition;

Comparative example 1

The difference between the comparative example and example 3 is that 1 part of blumea balsamifera extract is removed, and the magnolia bark extract is adjusted to 2 parts, and the rest is the same as example 3.

Comparative example 2

The difference between this comparative example and example 3 is that 1 part of the Magnolia bark extract was removed and 2 parts of the Blumeae Balsamiferae extract were adjusted, and the rest was the same as example 3.

Comparative example 3

The difference between this comparative example and example 3 is that the blumea balsamifera extract was adjusted to a blumea balsamifera extract, and the rest was the same as example 3.

Comparative example 4

The difference between this comparative example and example 3 is that the aqueous acetone solution in step (3) was adjusted to remove acetone and the solvent was adjusted to ethanol water, and the rest was the same as example 3.

Comparative example 5

The difference between this comparative example and example 3 is that the aqueous acetone solution in step (3) was adjusted to an aqueous ethanol solution, the extraction temperature was adjusted to 100 ℃, that is, the extraction method was heating reflux extraction, and the rest was the same as example 3.

Effect example 1 mosquito repellent effect test

Reference is made to GB/T17322.10-1998. The subjects were 64 persons, and 8 persons were divided into 8 groups, and the mosquito repellent compositions prepared in examples 1 to 3 and comparative examples 1 to 5 were tested, respectively. One hand of each person is blank control, and the other hand is drawn to show skin area of 5cm × 5cm at 1.5uL/cm²The dosage of the composition is uniform, exposing 4cm × 4cm part, tightly shielding the rest part, spreading for 2 hr, putting hand into cage containing mosquito for 2min, and observing whether there is mosquito coming or notBlood is sucked, and the test is carried out every 1h until 1 mosquito starts to attack, and the effective mosquito repelling time is recorded. The test results are given in table 1 below.

TABLE 1 mosquito repelling time chart for each group of products

Product group	Effective mosquito repelling (protecting) time
		Example 1	15.0h
Example 2	16.0h
		Example 3	15.0h
Comparative example 1	12.0h
		Comparative example 2	13.0h
Comparative example 3	10.0h
		Comparative example 4	11.0h
Comparative example 5	12.0h

Effect example 2 anti-inflammatory and antipruritic test

The test method comprises the following steps: 80 volunteers were selected and divided into 8 groups of 10 persons each according to a random number table, namely, examples 1 to 3 groups and comparative examples 1 to 5 groups. After the mosquito bites the skin, each group of products is respectively and uniformly smeared on the red and swollen part of the skin, and the average onset time of the itching relieving effect and the anti-inflammation and itching relieving effect of each group of mosquito repellent composition products are recorded. The results are shown in Table 2.

The curative effect grade is as follows:

the effect is shown: the itching symptom is completely removed, and the red swelling block is removed;

the method has the following advantages: the itching symptom is basically eliminated or weakened, and the skin red swelling is reduced;

and (4) invalidation: the skin pruritus symptom and the detumescence effect are not obvious or aggravated.

TABLE 2 anti-inflammatory and antipruritic test results

Effect example 3 Sterilization Effect test

The product is obtained by mixing (1-5). times.10⁴cfu/g of the bacterial solution was uniformly spread on a plate, and then the mosquito repellent compositions prepared in examples 1 to 3 and comparative examples 1 to 5 were uniformly spread on the surface of the plate and cultured for 8min, and the viable bacteria amount (cfu/g) on the plate was measured, and the bacterial survival amount was compared with the bacterial amount in the original bacterial solution to calculate the sterilization rate (%), and the results are shown in table 3.

TABLE 3 Sterilization results Table

The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention should be included in the technical scope of the present invention.

Claims

1. A natural galenical preparation composition with mosquito repelling and itching relieving functions is characterized by comprising the following raw materials: magnolia bark extract, blumea balsamifera extract, fructus cnidii extract, mint extract, citrus peel extract, pepper extract and ethanol;

the preparation method of the fructus cnidii extract comprises the following steps: extracting fructus Cnidii with 10-25% acetone water solution, filtering, concentrating, and drying to obtain fructus Cnidii extract;

the preparation method of the composition is characterized by comprising the following steps:

(2) respectively adding water into cortex Magnolia officinalis, herba Blumeae Balsamiferae, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, distilling with water vapor to extract volatile oil,

filtering, concentrating the filtrate to obtain concentrated solution, and mixing the volatile oil and concentrated solution to obtain extract of each medicinal material;

(3) mixing cortex Magnolia officinalis extract, herba Blumeae Balsamiferae extract, fructus Cnidii extract, herba Menthae extract, pericarpium Citri Tangerinae extract and fructus Zanthoxyli extract with ethanol.

2. The composition as claimed in claim 1, wherein the raw materials of the composition comprise the following components in parts by weight: 0.01-1.0 part of magnolia bark extract, 0.01-1.0 part of blumea balsamifera extract, 0.01-2.0 parts of common cnidium fruit extract, 0.02-2.0 parts of mint extract, 0.1-2.0 parts of orange peel extract, 0.5-1.5 parts of pepper extract and 0.2-1.0 part of ethanol.

3. The composition as claimed in claim 1, wherein the raw materials of the composition comprise the following components in parts by weight: 0.1-1.0 part of magnolia officinalis extract, 0.1-1.0 part of blumea balsamifera extract, 1-2 parts of fructus cnidii extract, 0.5-1 part of mint extract, 1-2 parts of citrus peel extract, 1.0-1.5 parts of pepper extract and 0.5-1.0 part of ethanol.

4. The composition of claim 3, wherein the ethanol is 95% ethanol.

5. The composition of claim 1, wherein the fructus Cnidii extract is extracted at a temperature of 40-50 deg.C.

6. The composition according to claim 1, wherein the leaching time is 2-4h, and the mass ratio of the 10-25% acetone aqueous solution to the fructus cnidii is 6-10: 1.

7. A process for preparing a composition according to any one of claims 1 to 6, characterized in that it comprises the following steps:

(2) respectively adding water into cortex Magnolia officinalis, herba Blumeae Balsamiferae, herba Menthae, pericarpium Citri Tangerinae and fructus Zanthoxyli, distilling with steam to extract volatile oil, filtering, concentrating the filtrate to obtain concentrated solution, and mixing the volatile oil and concentrated solution to obtain extracts;

8. The method according to claim 7, wherein in the step (1), the crushed mesh number is 10 to 65 mesh.

9. The preparation method according to claim 7, wherein in the step (2), the extraction time is 5-7h, and the mass ratio of the water to the medicinal material is 6-8: 1.

10. The method according to claim 7, wherein in the step (2), the concentration is carried out under reduced pressure at 50 to 60 ℃ until the relative density of the concentrated solution at 50 to 60 ℃ is 1.05 to 1.20.

11. Use of a natural galenical composition according to any one of claims 1 to 6 or a composition prepared by a preparation method according to any one of claims 7 to 10 in the preparation of a mosquito repellent product.