CN118103519A - 用于酶促核酸合成的方法 - Google Patents

用于酶促核酸合成的方法 Download PDF

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Publication number
CN118103519A
CN118103519A CN202280047556.9A CN202280047556A CN118103519A CN 118103519 A CN118103519 A CN 118103519A CN 202280047556 A CN202280047556 A CN 202280047556A CN 118103519 A CN118103519 A CN 118103519A
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CN
China
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lys
glu
ser
val
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Pending
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CN202280047556.9A
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English (en)
Chinese (zh)
Inventor
丹尼尔·奥尔森
东昕·卡伦·徐
萨布里纳·巴弗特
赫尔格·齐勒
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Primrose Biotech
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Primrose Biotech
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Publication date
Application filed by Primrose Biotech filed Critical Primrose Biotech
Publication of CN118103519A publication Critical patent/CN118103519A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN202280047556.9A 2021-06-14 2022-06-13 用于酶促核酸合成的方法 Pending CN118103519A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163210429P 2021-06-14 2021-06-14
US63/210,429 2021-06-14
PCT/US2022/033312 WO2022266019A2 (en) 2021-06-14 2022-06-13 Compositions and methods for enzymatic nucleic acid synthesis

Publications (1)

Publication Number Publication Date
CN118103519A true CN118103519A (zh) 2024-05-28

Family

ID=82403619

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202280047556.9A Pending CN118103519A (zh) 2021-06-14 2022-06-13 用于酶促核酸合成的方法
CN202280047599.7A Pending CN117881790A (zh) 2021-06-14 2022-06-13 用于酶促核酸合成的用途和方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN202280047599.7A Pending CN117881790A (zh) 2021-06-14 2022-06-13 用于酶促核酸合成的用途和方法

Country Status (9)

Country Link
US (2) US20240301457A1 (https=)
EP (2) EP4355894A2 (https=)
JP (2) JP2024522222A (https=)
KR (2) KR20240021866A (https=)
CN (2) CN118103519A (https=)
AU (1) AU2022293386A1 (https=)
IL (1) IL309044A (https=)
MX (2) MX2023014873A (https=)
WO (2) WO2022266019A2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026008711A2 (en) * 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
DE10215035A1 (de) 2002-04-05 2003-10-23 Roche Diagnostics Gmbh Rekombinante terminale Deoxynukleotidyltransferase mit verbesserter Funktionalität
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
JP6920275B2 (ja) * 2015-07-13 2021-08-18 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸を用いた回収可能な情報記憶のための方法
US12227775B2 (en) * 2017-05-22 2025-02-18 The Charles Stark Draper Laboratory, Inc. Modified template-independent DNA polymerase
GB201901501D0 (en) * 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
JP2020150801A (ja) * 2019-03-18 2020-09-24 国立大学法人東京工業大学 Ｄｎａの酵素合成法

Also Published As

Publication number Publication date
KR20240022552A (ko) 2024-02-20
WO2022266019A2 (en) 2022-12-22
WO2022266020A3 (en) 2023-03-09
JP2024522217A (ja) 2024-06-11
EP4355894A2 (en) 2024-04-24
EP4355895A2 (en) 2024-04-24
MX2023014874A (es) 2024-04-29
MX2023014873A (es) 2024-04-29
JP2024522222A (ja) 2024-06-11
WO2022266020A2 (en) 2022-12-22
WO2022266019A3 (en) 2023-02-02
CN117881790A (zh) 2024-04-12
IL309044A (en) 2024-02-01
US20240301457A1 (en) 2024-09-12
US20240384318A1 (en) 2024-11-21
KR20240021866A (ko) 2024-02-19
AU2022293386A1 (en) 2023-12-21

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