AU2022293386A1 - Methods for enzymatic nucleic acid synthesis - Google Patents

Methods for enzymatic nucleic acid synthesis Download PDF

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Publication number
AU2022293386A1
AU2022293386A1 AU2022293386A AU2022293386A AU2022293386A1 AU 2022293386 A1 AU2022293386 A1 AU 2022293386A1 AU 2022293386 A AU2022293386 A AU 2022293386A AU 2022293386 A AU2022293386 A AU 2022293386A AU 2022293386 A1 AU2022293386 A1 AU 2022293386A1
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AU
Australia
Prior art keywords
nucleic acid
polymerase
dna
substrate
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2022293386A
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English (en)
Inventor
Sabrina BAFFERT
Daniel Olson
Dongxin Karen XU
Helge Zieler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Primrose Bio Inc
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Primrose Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Primrose Bio Inc filed Critical Primrose Bio Inc
Publication of AU2022293386A1 publication Critical patent/AU2022293386A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AU2022293386A 2021-06-14 2022-06-13 Methods for enzymatic nucleic acid synthesis Pending AU2022293386A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163210429P 2021-06-14 2021-06-14
US63/210,429 2021-06-14
PCT/US2022/033312 WO2022266019A2 (en) 2021-06-14 2022-06-13 Compositions and methods for enzymatic nucleic acid synthesis

Publications (1)

Publication Number Publication Date
AU2022293386A1 true AU2022293386A1 (en) 2023-12-21

Family

ID=82403619

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2022293386A Pending AU2022293386A1 (en) 2021-06-14 2022-06-13 Methods for enzymatic nucleic acid synthesis

Country Status (9)

Country Link
US (2) US20240301457A1 (https=)
EP (2) EP4355894A2 (https=)
JP (2) JP2024522222A (https=)
KR (2) KR20240021866A (https=)
CN (2) CN118103519A (https=)
AU (1) AU2022293386A1 (https=)
IL (1) IL309044A (https=)
MX (2) MX2023014873A (https=)
WO (2) WO2022266019A2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2026008711A2 (en) * 2024-07-02 2026-01-08 Dna Script Filtering device and method for polynucleotide enzymatic synthesis

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
DE10215035A1 (de) 2002-04-05 2003-10-23 Roche Diagnostics Gmbh Rekombinante terminale Deoxynukleotidyltransferase mit verbesserter Funktionalität
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
JP6920275B2 (ja) * 2015-07-13 2021-08-18 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸を用いた回収可能な情報記憶のための方法
US12227775B2 (en) * 2017-05-22 2025-02-18 The Charles Stark Draper Laboratory, Inc. Modified template-independent DNA polymerase
GB201901501D0 (en) * 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
JP2020150801A (ja) * 2019-03-18 2020-09-24 国立大学法人東京工業大学 Ｄｎａの酵素合成法

Also Published As

Publication number Publication date
KR20240022552A (ko) 2024-02-20
WO2022266019A2 (en) 2022-12-22
WO2022266020A3 (en) 2023-03-09
JP2024522217A (ja) 2024-06-11
EP4355894A2 (en) 2024-04-24
EP4355895A2 (en) 2024-04-24
MX2023014874A (es) 2024-04-29
MX2023014873A (es) 2024-04-29
JP2024522222A (ja) 2024-06-11
WO2022266020A2 (en) 2022-12-22
WO2022266019A3 (en) 2023-02-02
CN117881790A (zh) 2024-04-12
IL309044A (en) 2024-02-01
US20240301457A1 (en) 2024-09-12
US20240384318A1 (en) 2024-11-21
KR20240021866A (ko) 2024-02-19
CN118103519A (zh) 2024-05-28

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