CN118000192A - Manufacturing method for efficient color retention of plant specimens - Google Patents

Manufacturing method for efficient color retention of plant specimens Download PDF

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Publication number
CN118000192A
CN118000192A CN202410133980.6A CN202410133980A CN118000192A CN 118000192 A CN118000192 A CN 118000192A CN 202410133980 A CN202410133980 A CN 202410133980A CN 118000192 A CN118000192 A CN 118000192A
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plants
plant
color
specimen
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范洋
逯小萌
王丽霞
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Puyang Food And Drug Inspection And Testing Center
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Puyang Food And Drug Inspection And Testing Center
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A61L2/0029Radiation
    • A61L2/0047Ultraviolet radiation
    • GPHYSICS
    • G09EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09BEDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/38Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for botany

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
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  • Mathematical Analysis (AREA)
  • Pure & Applied Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Toxicology (AREA)
  • Algebra (AREA)
  • Computational Mathematics (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Mathematical Optimization (AREA)
  • Mathematical Physics (AREA)
  • Dentistry (AREA)
  • Business, Economics & Management (AREA)
  • Educational Administration (AREA)
  • Educational Technology (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
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  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a method for manufacturing a plant specimen with high efficiency and color retention, and relates to the technical field of preparation of plant specimens. The invention comprises the following steps: s1: and (3) material collection: collecting fresh plants or branches without diseases and with complete branches and leaves; s2: pretreatment of materials: trimming and washing the collected branches and plants; s3: and (3) sterilization: sterilizing the washed plants and branches; s4: preparing a color retention stock solution; s5: and (3) plant color preservation treatment: diluting the color-preserving mother solution, heating to boiling, putting the sterilized plants and branches into a reaction vessel, continuously heating, and taking out the plants and branches; s6: and (3) freeze drying: placing the plants and branches after color preservation in a specimen holder, flattening and freeze-drying; s7: and (3) laminating a specimen: flattening the frozen and dried plants and branches, and placing the plants and branches into a plastic packaging machine for plastic packaging by using a card protection film. The plant specimen can keep leaves green for a long time, and the ornamental value and research value of the plant specimen are improved.

Description

Manufacturing method for efficient color retention of plant specimens
Technical Field
The invention relates to the technical field of plant specimen preparation, in particular to a method for manufacturing a plant specimen with high efficiency and color retention.
Background
The whole plant or a part of the whole plant is collected and then stored for observation at any time, and the whole plant is called a plant specimen, wherein the plant specimen is a teaching material capable of visually representing the shape and characteristics of the plant, is a material basis for plant classification work, is also an important basis for protecting the quality of the plant and identifying the plant species, and is relatively stable in color and difficult to fade in the long-term storage process of blue, purple, yellow and red flower plant specimens, but green leaves are browned, so that a plurality of precious specimens lack scientific research value and ornamental value.
The plant is green because chlorophyll is contained in chloroplasts of the plant, the chlorophyll is a complex organic compound, the center of the molecular structure of the chlorophyll is provided with a metal magnesium atom, the chlorophyll is green because the chlorophyll in plant leaves is easy to fade due to the core structure containing the magnesium atom, the green color of the plant specimen is faded, the green color of the leaves is required to be kept for a long time, and therefore, a preparation method for efficiently keeping the color of the plant specimen is needed to solve the problems.
Disclosure of Invention
The invention aims to solve the technical problems and provide the manufacturing method for efficiently preserving the color of the plant specimen, so that the plant specimen can keep green leaves for a long time, and the ornamental value and research value of the plant specimen are improved.
In order to solve the technical problems, the technical scheme provided by the invention is as follows: a manufacturing method for efficiently preserving color of a plant specimen comprises the following steps:
S1: and (3) material collection: collecting fresh plants or branches without diseases and with complete branches and leaves;
s2: pretreatment of materials: trimming or reducing thick stems and thorn-bearing stem whiskers in the picked branches, partially removing green leaves of the picked plants according to the growth characteristics of the leaves, and flushing the trimmed plants and branches with distilled water;
s3: and (3) sterilization: placing the washed plants and branches under ultraviolet lamps for sterilization treatment for 20-50 minutes;
S4: preparing a color retention stock solution: adding copper acetate crystals into a 50% glacial acetic acid aqueous solution, heating until the copper acetate crystals and the glacial acetic acid aqueous solution are not dissolved any more, and then cooling the color retention stock solution to obtain a color retention mother solution for later use;
S5: and (3) plant color preservation treatment: pouring 1 part of color retention mother liquor into a reaction vessel, adding 3-4 parts of water into the reaction vessel, diluting the color retention mother liquor, heating the diluted color retention mother liquor to boiling, then putting sterilized plants and branches into the reaction vessel, continuing heating, gradually turning green parts of the plants and branches into brown, continuing heating, turning the plants and branches into green again, taking out the plants and branches, putting the plants and branches into cold water for cooling for 5-10 minutes, fully washing the plants and branches with clear water, and sucking water with water absorption paper;
S6: and (3) freeze drying: placing the plants and branches after color preservation in a specimen holder, flattening, pre-cooling for 10-30 minutes at the temperature of minus 10 ℃ to minus 5 ℃, pre-cooling for 30-60 minutes at the temperature of minus 15 ℃ to minus 10 ℃, and drying the pre-cooled plants in vacuum for 15-25 hours;
S7: and (3) laminating a specimen: placing the freeze-dried plants and branches on card paper, flattening, placing into a plastic packaging machine, and plastic packaging with a card protection film at 150-180 ℃ to obtain the color-protected plant specimen.
Preferably, the plant specimen is a specimen of a grass leaf type green leaf plant, a membranous leaf type green leaf plant, a revolutionary leaf type green leaf plant, and a fleshy leaf type green leaf plant.
Preferably, the reaction vessel in S5 includes a glass vessel, a stainless steel vessel, and a ceramic vessel.
Preferably, in the step S6, when the plants and the branches are placed in the specimen holder, the plants and the branches are placed on the grass paper, the morphology of the plants and the branches is finished, and the leaves are unfolded.
Preferably, the thickness of the card paper in the step S7 is 0.2-0.4 mm, and the thickness of the card protection film is 0.15-0.20 mm.
After adopting the structure, the invention has the following advantages:
1. the invention adopts ultraviolet lamp to sterilize for a specific time before the specimen is manufactured, and utilizes the strong bactericidal power of ultraviolet rays to kill various bacterial propagules (including tubercle bacillus), fungi and viruses and bacterial spores with strong resistance to physical and chemical factors, thereby prolonging the preservation time of the specimen;
2. the method has the advantages of simple and convenient technical operation, easily obtained required materials, low cost and short treatment process, and provides theoretical and practical basis for green leaf color preservation of plant specimens;
3. According to the invention, copper acetate crystals and 50% glacial acetic acid aqueous solution are adopted to prepare the color retention mother solution according to a specific mass ratio, and the copper acetate crystals are added to replace magnesium ions in chlorophyll by copper ions, so that new chlorophyll molecules which are stable to store and take the copper ions as the center can be formed, and the green color can be stored for a long time;
4. The invention has wide application range, can be applied to the color preservation of green leaves of grass leaf plants, green leaves of membranous leaf plants, green leaves of revolutionary leaf plants and green leaves of fleshy leaf plants, has little change of browning rate of leaves after long-time preservation, can realize long-term preservation of the color of green leaves of plant specimens, and improves scientific research value, artistic value and ornamental value of the plant specimens.
The foregoing summary is for the purpose of the specification only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present invention will become apparent by reference to the drawings and the following detailed description.
Drawings
FIG. 1 is a process flow diagram of a method for efficiently preserving color of a plant specimen.
In order to more clearly illustrate the embodiments of the application or the technical solutions in the prior art, the drawings that are necessary for the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the application and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
Detailed Description
Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those of skill in the pertinent art, the described embodiments may be modified in various different ways without departing from the spirit or scope of the present invention. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive.
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
Collecting fresh plants or branches without diseases and with complete branches and leaves;
Trimming or reducing thick stems and thorn-bearing stem whiskers in the picked branches, partially removing green leaves of the picked plants according to the growth characteristics of the leaves, and flushing the trimmed plants and branches with distilled water;
placing the washed plants and branches under ultraviolet lamps for sterilization treatment for 20 minutes;
adding copper acetate crystals into a 50% glacial acetic acid aqueous solution, heating until the copper acetate crystals and the glacial acetic acid aqueous solution are not dissolved any more, and then cooling the color retention stock solution to obtain a color retention mother solution for later use;
Pouring 1 part of color retention mother liquor into a reaction vessel, wherein the reaction vessel is a glass container, adding 3 parts of water into the reaction vessel, diluting the color retention mother liquor, heating the diluted color retention mother liquor to boiling, then putting sterilized plants and branches into the reaction vessel, continuing heating, gradually turning green parts of the plants and branches into brown, continuing heating, turning the plants and branches into green again, taking out the plants and branches, putting the plants and branches into cold water for cooling for 5 minutes, fully washing the plants and branches with clear water, and drying the water by using absorbent paper;
Placing the plants and branches after color preservation in a specimen holder for flattening, firstly placing the plants and branches on grass paper when the plants and branches are placed in the specimen holder, finishing the forms of the plants and branches, expanding leaves, pre-cooling for 10 minutes at the temperature of minus 10 ℃, pre-cooling for 30 minutes at the temperature of minus 15 ℃, and drying the pre-cooled plants in vacuum for 15 hours;
And placing the freeze-dried plants and branches on card paper, wherein the thickness of the card paper is 0.2 mm, flattening, placing into a plastic packaging machine, and using a card protection film for plastic packaging, wherein the thickness of the card protection film is 0.15 mm, and the temperature of the plastic packaging card protection film is 150 ℃, so that the color-protected plant specimen can be obtained.
Example two
Collecting fresh plants or branches without diseases and with complete branches and leaves;
Trimming or reducing thick stems and thorn-bearing stem whiskers in the picked branches, partially removing green leaves of the picked plants according to the growth characteristics of the leaves, and flushing the trimmed plants and branches with distilled water;
placing the washed plants and branches under ultraviolet lamps for sterilization treatment for 35 minutes;
adding copper acetate crystals into a 50% glacial acetic acid aqueous solution, heating until the copper acetate crystals and the glacial acetic acid aqueous solution are not dissolved any more, and then cooling the color retention stock solution to obtain a color retention mother solution for later use;
Pouring 1 part of color retention mother liquor into a reaction vessel, wherein the reaction vessel is a stainless steel container, adding 4 parts of water into the reaction vessel, diluting the color retention mother liquor, heating the diluted color retention mother liquor to boiling, then putting sterilized plants and branches into the reaction vessel, continuously heating, gradually turning green parts of the plants and branches into brown, continuously heating, turning the plants and branches into green again, taking out the plants and branches, putting the plants and branches into cold water for cooling, fully flushing the plants and branches with clear water, and drying the water by using water absorption paper;
Placing the plants and branches after color preservation in a specimen holder for flattening, firstly placing the plants and branches on grass paper when the plants and branches are placed in the specimen holder, finishing the forms of the plants and branches, expanding leaves, pre-cooling for 20 minutes at the temperature of minus 17 ℃, pre-cooling for 45 minutes at the temperature of minus 13 ℃, and drying the pre-cooled plants in vacuum for 20 hours;
And placing the freeze-dried plants and branches on card paper, wherein the thickness of the card paper is 0.3 millimeter, flattening, placing into a plastic packaging machine, and using a card protection film for plastic packaging, wherein the thickness of the card protection film is 0.17 millimeter, and the temperature of the plastic packaging card protection film is 170 ℃, so that the plant specimen after color protection can be obtained.
Example III
Collecting fresh plants or branches without diseases and with complete branches and leaves;
Trimming or reducing thick stems and thorn-bearing stem whiskers in the picked branches, partially removing green leaves of the picked plants according to the growth characteristics of the leaves, and flushing the trimmed plants and branches with distilled water;
placing the washed plants and branches under ultraviolet lamps for sterilization treatment, wherein the sterilization time is 50 minutes;
adding copper acetate crystals into a 50% glacial acetic acid aqueous solution, heating until the copper acetate crystals and the glacial acetic acid aqueous solution are not dissolved any more, and then cooling the color retention stock solution to obtain a color retention mother solution for later use;
Pouring 1 part of color retention mother liquor into a reaction vessel, wherein the reaction vessel is a ceramic container, adding 4 parts of water into the reaction vessel, diluting the color retention mother liquor, heating the diluted color retention mother liquor to boiling, then putting sterilized plants and branches into the reaction vessel, continuing heating, gradually turning green parts of the plants and branches into brown, continuing heating, turning the plants and branches into green again, taking out the plants and branches, putting the plants and branches into cold water for cooling for 10 minutes, fully washing the plants and branches with clear water, and drying the water by using absorbent paper;
Placing the plants and branches after color preservation in a specimen holder for flattening, firstly placing the plants and branches on grass paper when the plants and branches are placed in the specimen holder, finishing the forms of the plants and branches, expanding leaves, pre-cooling for 30 minutes at the temperature of minus 5 ℃, pre-cooling for 60 minutes at the temperature of minus 10 ℃, and drying the pre-cooled plants in vacuum for 25 hours;
And placing the freeze-dried plants and branches on card paper, wherein the thickness of the card paper is 0.4 mm, flattening, placing into a plastic packaging machine, and using a card protection film for plastic packaging, wherein the thickness of the card protection film is 0.20 mm, and the temperature of the plastic packaging card protection film is 180 ℃, so that the color-protected plant specimen can be obtained.
The invention and its embodiments have been described above with no limitation, and the actual examples are not limited thereto, as are shown in the figures as being only one of the embodiments of the invention. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present invention.

Claims (5)

1. A method for manufacturing a plant specimen with high efficiency and color retention is characterized in that: the method comprises the following steps:
S1: and (3) material collection: collecting fresh plants or branches without diseases and with complete branches and leaves;
s2: pretreatment of materials: trimming or reducing thick stems and thorn-bearing stem whiskers in the picked branches, partially removing green leaves of the picked plants according to the growth characteristics of the leaves, and flushing the trimmed plants and branches with distilled water;
s3: and (3) sterilization: placing the washed plants and branches under ultraviolet lamps for sterilization treatment for 20-50 minutes;
S4: preparing a color retention stock solution: adding copper acetate crystals into a 50% glacial acetic acid aqueous solution, heating until the copper acetate crystals and the glacial acetic acid aqueous solution are not dissolved any more, and then cooling the color retention stock solution to obtain a color retention mother solution for later use;
S5: and (3) plant color preservation treatment: pouring 1 part of color retention mother liquor into a reaction vessel, adding 3-4 parts of water into the reaction vessel, diluting the color retention mother liquor, heating the diluted color retention mother liquor to boiling, then putting sterilized plants and branches into the reaction vessel, continuing heating, gradually turning green parts of the plants and branches into brown, continuing heating, turning the plants and branches into green again, taking out the plants and branches, putting the plants and branches into cold water for cooling for 5-10 minutes, fully washing the plants and branches with clear water, and sucking water with water absorption paper;
S6: and (3) freeze drying: placing the plants and branches after color preservation in a specimen holder, flattening, pre-cooling for 10-30 minutes at the temperature of minus 10 ℃ to minus 5 ℃, pre-cooling for 30-60 minutes at the temperature of minus 15 ℃ to minus 10 ℃, and drying the pre-cooled plants in vacuum for 15-25 hours;
S7: and (3) laminating a specimen: placing the freeze-dried plants and branches on card paper, flattening, placing into a plastic packaging machine, and plastic packaging with a card protection film at 150-180 ℃ to obtain the color-protected plant specimen.
2. The method for producing a plant specimen with high efficiency and color retention according to claim 1, wherein the plant specimen is a specimen of a grass leaf type green leaf plant, a membranous leaf type green leaf plant, a revolutionary leaf type green leaf plant and a fleshy leaf type green leaf plant.
3. The method for efficiently preserving the color of a plant specimen according to claim 1, which is characterized in that: the reaction vessel in S5 comprises a glass vessel, a stainless steel vessel and a ceramic vessel.
4. The method for efficiently preserving the color of a plant specimen according to claim 1, which is characterized in that: in the step S6, when the plants and the branches are placed in the specimen holder, the plants and the branches are placed on the grass paper, the forms of the plants and the branches are tidied, and the leaves are unfolded.
5. The method for efficiently preserving the color of a plant specimen according to claim 1, which is characterized in that: the thickness of the card paper in the S7 is 0.2-0.4 mm, and the thickness of the card protection film is 0.15-0.20 mm.
CN202410133980.6A 2022-10-27 2022-10-27 Manufacturing method for efficient color retention of plant specimens Pending CN118000192A (en)

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CN202211322394.3A CN115530158A (en) 2022-10-27 2022-10-27 Method for making plant specimen with high-efficiency color retention

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Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
CN1214202A (en) * 1997-10-14 1999-04-21 陈军 Making method of plant specimen with covered color-protecting film
JP5355061B2 (en) * 2008-04-01 2013-11-27 井室 隆 Method for producing dried florets that do not change color from easily discolored florets
CN104472475B (en) * 2014-11-25 2016-08-17 贵州大自然科技股份有限公司 A kind of vacuum refrigeration technique of palm petal
CN107027745A (en) * 2017-04-24 2017-08-11 浙江海洋大学 A kind of preparation method of plant colouring sample
CN110432262B (en) * 2019-07-10 2021-06-08 浙江海洋大学 Preparation and preservation method of plant cured leaf specimen
CN111721073B (en) * 2020-06-22 2022-03-25 银谷芳香科技有限公司 Vacuum freeze-drying rose flower process
CN112640893B (en) * 2020-12-16 2022-08-19 赣南树木园 Manufacturing method of sightseeing wood color-preserving specimen

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