CN109757436B - Method for obtaining high-activity honey - Google Patents
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- CN109757436B CN109757436B CN201910019415.6A CN201910019415A CN109757436B CN 109757436 B CN109757436 B CN 109757436B CN 201910019415 A CN201910019415 A CN 201910019415A CN 109757436 B CN109757436 B CN 109757436B
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Abstract
The invention discloses a method for obtaining high-activity honey, which comprises the steps of selecting antibacterial and sterile carbonized poplar as a bee colony living place after strict environment and bee colony screening, controlling the activity cycle of bees through manual temperature control so as to achieve the technical purpose of equal honey composition in each cycle, simultaneously stirring and degassing in a vacuum sterile manner so as to greatly reduce the oxygen content in natural active honey, inhibiting the action of oxidase, and effectively preventing the active honey containing high water content from losing efficacy due to the fact that excessive polysaccharide is converted into hydrogen peroxide under the action of the oxidase. The invention has the characteristics of pure nature, high enzyme, high activity and uniform quality.
Description
Technical Field
The invention relates to the technical field of bee keeping, in particular to a method for obtaining high-activity honey.
Background
Active honey: the honey is prepared from mature honey with natural activity, and has higher enzyme value, undamaged active substances and higher nutritive value compared with concentrated honey. The active natural mature honey is sweet substance which is obtained by collecting nectar or secretion of plants by bees and stored in honeycomb after being fully brewed by the bees. This is the concept of active natural ripe honey, and also illustrates the source of active natural ripe honey: the active natural mature honey can not be brewed by bees, plants and bees, and can not be counted as the active natural mature honey after the conditions are left, so the active natural mature honey cannot be artificially made.
Concentrated honey with various meshes is sold in the market at present, and after the honey is heated and concentrated, the crystal nucleus of the honey is damaged, so that the concentrated honey is not crystallized or is rarely crystallized, and looks more like selling; the ultra-high concentration of the concentrated honey label attracts more than one person to buy, the concentrated honey is considered to be good honey, and the active substances of the honey are greatly unknown to be damaged, so that the effect is greatly reduced; in addition, the concentrated honey does not generate gas and is easy to store, and consumers can normally feel that the bottle does not swell after buying the concentrated honey. The active honey can generate gas, the bottle can expand at a higher temperature, and the bottle mouth still has foam, so that consumers are doubtful. After the concentrated honey is concentrated at high temperature, active substances are destroyed, even the most important effect of relaxing bowel is very poor, other health care effects are more discounted, and the health care value is almost the same.
Therefore, the honey with high activity and uniform quality and high activity of the pure natural honey enzyme and the obtaining method thereof are urgently needed in the market.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the pure natural high-activity high-melinase honey with high activity and uniform quality and the obtaining method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme: a method for obtaining high-activity honey comprises the following steps:
1) preparation before bee rearing
Matching environments: finding woody flower plants with the density of 0.1 plant/m in the area of 20-25 degrees of latitude in China22 strains/m2And the area with the annual average wind speed of 0.5-0.8 m/s is used as a bee-keeping site;
matching bee colonies: from 3 months and 1 days to 4 months and 30 days, taking the Chinese bee colonies corresponding to the regions as sample objects, and searching natural bee colonies which always keep strong colonies as target colonies;
matching the honeycomb: taking a carbonized poplar trunk with the diameter of 30-60 cm and the length of 70-100 cm in each section as a honeycomb root, removing barks, hollowing the middle part, keeping the wall thickness of 5-10 cm, and then cutting each section into two halves along the axis, wherein each section is used as a honeycomb root;
preparing a honeycomb; preparing a transparent glass honeycomb which is provided with push-open glass windows on the periphery, is internally provided with a temperature control device and is provided with vent holes on the side surface close to the bottom;
preparing equipment: preparing a vacuum mechanical stirring device with a glass stirring head and preparing a glass container;
2) bee breeding
Picking stage 1), selecting a natural honeycomb with queen cells in a target community;
arranging one honeycomb according to the square kilometer, wherein each natural honeycomb corresponds to one honeycomb, and the proportion of 3-5 honeycomb roots is arranged in each honeycomb, manually transplanting the natural honeycomb obtained in the step I into the honeycomb with windows opened on four sides, arranging all the honeycomb roots close to the natural honeycomb, then trapping the original honeycomb, performing distribution operation after the outer end of the original queen cell is brown, moving the original queen cell to the central position of a hollow area of the honeycomb root, then repeating the operation after the natural honeycomb continues to be manufactured until all the honeycomb roots are inoculated with new queen cells and the bee colony is naturally grouped, returning the natural honeycomb to the original position, arranging the honeycomb roots close to the window in the honeycomb and far away from each other, and waiting for the natural development of the bee colony;
thirdly, after all the bee colonies grow to 1/2-2/3 of the number of the original natural bee colony, 2/3 of all the artificial honeycombs are taken out for primary brewing of the honey and discarded, then a temperature control device of the honeycomb is opened, a window is closed, air vents are kept unblocked, the temperature in the honeycomb is controlled to be 18-22 ℃, the time lasts for 1 day, then the temperature control device is closed, the window is opened, and the first harvesting period is started;
opening every 5-7 days and closing for 1 day to form a cycle, taking honey in 2/3-3/4 in the next morning 7:00-8:00 after the closing day of each time in the high honey flow period, repeating the temperature control operation in the third step on each closed day in the high honey flow period, taking no honey in the honey lack period, and feeding artificially according to the conditions to obtain high-equal raw honey;
3) post-treatment
Placing the high-equality raw honey obtained in the step (2) in the step (1) in a vacuum mechanical stirring device prepared in the step (1), continuously stirring for 2-2.5 h at the stirring speed of 20-25 rpm/min and the vacuum degree of 0.5-2 Pa, and taking out to obtain the primary honey to be treated;
secondly, a 120-mesh bamboo carbon fiber net is adopted for primary filtration to obtain undoped honey;
thirdly, heating the undoped honey obtained in the second step to 55-60 ℃, keeping the temperature for 15-18 min, and then quickly cooling to 3-5 ℃ to obtain pure honey;
fourthly, the pure honey obtained in the third step is taken out and filled into the glass container prepared in the fifth step of the step 1) to be sealed, and then the required high-activity honey is obtained.
Compared with the prior art, the invention has the following advantages due to the adoption of the scheme: (1) the invention adopts bamboo carbon fiber for filtration, firstly, the bamboo carbon has super strong adsorption capacity to harmful substances such as sulfide, nitride, formaldehyde, benzene, phenol and the like, and the adsorption capacity is three times of that of activated carbon; the bamboo charcoal can also remove strong oxidation components and various harmful impurities in the honey, the purity of the honey is improved on the basis of not damaging the intrinsic activity of the honey, and the composite pasteurization method can more durably and effectively maintain the activity of the honey. (2) The reason for the specially selected area and the matched annual average wind speed is that the area environment and the wind speed are suitable for bee colony survival (the wind speed is not high, so that the flying is difficult, the wind speed is not low, so that the trees can be bred into a forest, and the environment is not possible by blowing and pollinating only by wind, so that a dense bee colony is inevitable, the proportion of strong colonies in the bee colony is large, and the bee colony is suitable for bee keeping, particularly natural bees) (3) the honeycomb is arranged in the carbonized poplar trunk, is not randomly arranged, the poplar trunk is usually straight, the trunk has insect expelling and anti-residing effects, and has the effects of humidity regulation and disinfection and warm keeping after carbonization, is more suitable for southern high-humidity climate, is longer in service life, and is the best choice for long-term bee keeping. (4) The core idea of the invention is temperature control quality, in the prior art, no matter what technology is used, the cost is increased, the honey collection amount is reduced, but in fact, in order to ensure the quality of honey and the realizability of post-processing, in order to ensure that the finished honey still has considerable activity for a long time after being treated, the invention strictly controls the activity period of bee colony, so that the honey obtained in each period has relatively consistent components (the first day of each period produces honey with the water content being the last after long-time brewing, and the last day is honey with the water content being about 50 percent, and after one day of pure brewing, the honey is uniformly stirred, the invention finally obtains cooked honey with the water content being about 18 percent, only after vacuum degassing, the honey is immediately encapsulated, and the hydrogen peroxide floating on the surface of the honey produced in the process of brewing is utilized for sterilization and natural quality guarantee, the honey with higher water content can keep sufficient activity, and the influence on the taste and the acceleration of fermentation and deterioration caused by overhigh water content can be avoided), which is the necessary quality control basis for mature industrialized food production. (5) The latitude selected by the invention corresponds to the corresponding areas of southern China, Guangxi Zhuang autonomous region, Guangdong province and Taiwan province, the temperature of the areas is relatively balanced all the year round (at 25-30 ℃ for a long time), and the plant varieties, particularly woody flower plants, are various, have long flowering phase and rich nutrition, thus being the environmental basis for realizing high-quality honey production. (6) The invention relates to a method for obtaining honey by selecting the next morning of a closed day as the only honey taking period of the invention, which is not the time selected randomly, and the principle of the invention lies in that the applicant finds out accidentally that in the high honey flow period, the honey taking in the morning does not affect the survival of bee colonies, but promotes the bee colonies to have more activity and is less prone to honey division, and the day of the closed day is not only used for dividing a period to obtain honey with more equal quality, but is different from the high baume degree obtained by high-temperature concentration counterfeiting in the prior art and is naturally obtained by the natural characteristics of bees under manual operation, so that the method has the following advantages that: in autumn, the bees still have a small amount of honey, although the yield is very low, the quality of the honey is very high, long-term observation shows that during the temperature of 18-22 ℃ adopted by the honey producing device, the bees can go out less, but the honey is not required to be agglomerated and heated when the temperature is too low, most of the bees are statically lodged in the honeycomb and produce the honey, although the consumption of the original honey is higher, the maturity of the honey is greatly increased, and the honey producing device can also obtain high-quality high-activity honey in a high honey flow period in such a way. (7) The method selects Chinese bees in an original production area instead of Italian bees (imported Italy bees and the current domestic artificial bee species), which have larger honey yield and stronger viability, and mainly discovers that the cold resistance and the hostility resistance of the Chinese bees are far higher than those of western bees according to research, the total honey yield is less, but the activity is higher, the health care effect is more obvious, and moreover, for some plants flowering in winter, if the Chinese bees are pollinated, the survival is inevitably influenced, many plants in China are bred, the Chinese bees have no work, the pollination rate of the Chinese bees for apples is 30% higher than that of the western bees, and the Chinese bees have low temperature resistance, early attendance and good collection of sporadic honey sources, so that the method is an essential basis for realizing long-term continuous production operation in one area.
Detailed Description
Example 1:
a method for obtaining high-activity honey comprises the following steps:
1) preparation before bee rearing
Matching environments: finding a natural forest in Lingshan county of autonomous region of Guangxi Zhuang nationality, wherein woody flowers and plants take litchi trees asMainly, the density of flower plants is 0.3 plants/m2The annual average wind speed is 0.5m/s, and the bee-keeping place is used;
matching bee colonies: from 3 months and 1 days to 4 months and 30 days, taking the Chinese bee colonies corresponding to the regions as sample objects, and searching natural bee colonies which always keep strong colonies as target colonies;
matching the honeycomb: taking a main stem of a carbonized poplar with the diameter of 60cm and the length of 100cm of each section as a honeycomb root, removing barks, hollowing the middle part of the poplar, keeping the wall thickness of 10cm, and then cutting each section into two halves along an axis to respectively serve as the honeycomb root;
preparing a honeycomb; preparing a transparent glass honeycomb which is provided with push-open glass windows on the periphery, is internally provided with a temperature control device and is provided with vent holes on the side surface close to the bottom;
preparing equipment: preparing a vacuum mechanical stirring device with a glass stirring head and preparing a glass container;
2) bee breeding
Picking stage 1), selecting a natural honeycomb with queen cells in a target community;
arranging one honeycomb according to the square kilometer, wherein each natural honeycomb corresponds to one honeycomb, and the proportion of 5 honeycomb roots is arranged in each honeycomb, manually transplanting the natural honeycomb obtained in the step I into the honeycomb with windows opened on four sides, arranging all the honeycomb roots close to the natural honeycomb, trapping the original honeycomb, then carrying out distribution operation after the outer end of the original queen cell is brown, moving the original queen cell to the central position of a hollow area of the honeycomb root, then repeating the operation after the natural honeycomb continues to produce the queen cell until all the honeycomb roots are provided with new queen cells for breeding the honeycomb and the honeycomb is naturally grouped, returning the natural honeycomb to the original position, then arranging the honeycomb roots close to the window in the honeycomb and far away from each other, and standing until the honeycomb naturally develops;
thirdly, after all the bee colonies grow to 2/3 of the number of the original natural bee colony, taking out 2/3 initial honey in all the artificial honeycombs and discarding the initial honey, then opening a temperature control device of the bee hive, closing a window, keeping ventilation holes unblocked, controlling the temperature in the bee hive to be 18-22 ℃, continuing for 1 day, then closing the temperature control device, opening the window, and entering a first harvesting period;
fourthly, the opening is carried out for 1 day every 7 days to form a cycle, honey is taken from 3/4 in the high honey flow period, the temperature control operation of the third step is repeated on each closed day in the high honey flow period, honey is not taken in the honey lack period, and the honey is fed manually according to the condition, so that high-equal raw honey is obtained;
3) post-treatment
Placing the high-equality raw honey obtained in the step (2) in the step (1) in the prepared vacuum mechanical stirring device, continuously stirring for 2.5 hours at the stirring speed of 25rpm/min and the vacuum degree of 1Pa-2Pa, and taking out to obtain primary honey to be treated;
secondly, a 120-mesh bamboo carbon fiber net is adopted for primary filtration to obtain undoped honey;
thirdly, heating the undoped honey obtained in the second step to 55-60 ℃, keeping the temperature for 15-18 min, and then quickly cooling to 3-5 ℃ to obtain pure honey;
fourthly, the pure honey obtained in the third step is taken out and filled into the glass container prepared in the fifth step of the step 1) to be sealed, and then the required high-activity honey is obtained.
The active honey produced according to the example has a water content of 17.5%, a Baume degree of 42.1-42.5, an HMF of 3mg/kg-5mg/kg, an amylase value of 19.5, no detectable sucrose content, a sugar content of 74.2% -77.1%, no detectable chloramphenicol, and no tetracyclines.
Example 2:
the whole is in accordance with example 1, with the difference that:
a method for obtaining high-activity honey comprises the following steps:
1) preparation before bee rearing
Matching environments: finding a Yunnan mountain tea garden with the plant density of 2 plants/m in Yunnan Qujing2The annual average wind speed is 0.8m/s, and the bee-keeping place is used;
matching the honeycomb: taking a main stem of a carbonized poplar, which has the diameter of 30cm and the length of 70cm of each section, as a honeycomb root, removing barks, hollowing the middle part of the poplar, keeping the wall thickness of 5cm, and then cutting each section into two halves along an axis, wherein each section is used as a honeycomb root;
2) bee breeding
Arranging one honeycomb according to kilometer per square, wherein each natural honeycomb corresponds to one honeycomb, and the proportion of 3 honeycomb roots in each honeycomb is … …;
thirdly, after all the bee colonies grow to 1/2 of the number of the original natural bee colony, taking out 2/3 initial honey in all the artificial honeycombs and discarding the initial honey, then opening a temperature control device of the bee hive, closing a window, keeping ventilation holes unblocked, controlling the temperature in the bee hive to be 18-22 ℃, continuing for 1 day, then closing the temperature control device, opening the window, and entering a first harvesting period;
fourthly, the opening is carried out for 1 day every 5 days to form a cycle, honey is taken from 2/3 in the high honey flow period, the temperature control operation of the third step is repeated on each closed day in the high honey flow period, honey is not taken in the honey lack period, and the honey is fed manually according to the condition, so that high-equal raw honey is obtained;
3) post-treatment
Placing the high-equality raw honey obtained in the step (2) in the step (1) in the prepared vacuum mechanical stirring device, continuously stirring for 2 hours at the stirring speed of 20rpm/min and the vacuum degree of 0.5Pa-1Pa, and taking out to obtain primary honey to be treated;
the active honey produced according to the embodiment has the water content of 18.6 percent, the Baume degree of 42.8-43.1, the HMF3mg/kg-5mg/kg, the amylase value of 17.3, the sucrose content of undetected, the sugar content of 68.8-70.3 percent, the chloramphenicol of undetected and the tetracycline of undetected.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (1)
1. A method for obtaining high-activity honey is characterized by comprising the following steps:
1) preparation before bee rearing
Matching environments: finding woody flower plants with the density of 0.1 plant/m in the area of 20-25 degrees of latitude in China22 strains/m2And the area with the annual average wind speed of 0.5-0.8 m/s is used as a bee-keeping site;
matching bee colonies: from 3 months and 1 days to 4 months and 30 days, taking the Chinese bee colonies corresponding to the regions as sample objects, and searching natural bee colonies which always keep strong colonies as target colonies;
matching the honeycomb: taking a carbonized poplar trunk with the diameter of 30-60 cm and the length of 70-100 cm in each section as a honeycomb root, removing barks, hollowing the middle part, keeping the wall thickness of 5-10 cm, and then cutting each section into two halves along the axis, wherein each section is used as a honeycomb root;
preparing a honeycomb; preparing a transparent glass honeycomb which is provided with push-open glass windows on the periphery, is internally provided with a temperature control device and is provided with vent holes on the side surface close to the bottom;
preparing equipment: preparing a vacuum mechanical stirring device with a glass stirring head and preparing a glass container;
2) bee breeding
Picking stage 1), selecting a natural honeycomb with queen cells in a target community;
arranging one honeycomb according to the square kilometer, wherein each natural honeycomb corresponds to one honeycomb, and the proportion of 3-5 honeycomb roots is arranged in each honeycomb, manually transplanting the natural honeycomb obtained in the step I into the honeycomb with windows opened on four sides, arranging all the honeycomb roots close to the natural honeycomb, then trapping the original honeycomb, performing distribution operation after the outer end of the original queen cell is brown, moving the original queen cell to the central position of a hollow area of the honeycomb root, then repeating the operation after the natural honeycomb continues to be manufactured until all the honeycomb roots are inoculated with new queen cells and the bee colony is naturally grouped, returning the natural honeycomb to the original position, arranging the honeycomb roots close to the window in the honeycomb and far away from each other, and waiting for the natural development of the bee colony;
thirdly, after all the bee colonies grow to 1/2-2/3 of the number of the original natural bee colony, 2/3 of all the artificial honeycombs are taken out for primary brewing of the honey and discarded, then a temperature control device of the honeycomb is opened, a window is closed, air vents are kept unblocked, the temperature in the honeycomb is controlled to be 18-22 ℃, the time lasts for 1 day, then the temperature control device is closed, the window is opened, and the first harvesting period is started;
opening every 5-7 days and closing for 1 day to form a cycle, taking honey in 2/3-3/4 in the next morning 7:00-8:00 after the closing day of each time in the high honey flow period, repeating the temperature control operation in the third step on each closed day in the high honey flow period, taking no honey in the honey lack period, and feeding artificially according to the conditions to obtain high-equal raw honey;
3) post-treatment
Placing the high-equality raw honey obtained in the step (2) in the step (1) in a vacuum mechanical stirring device prepared in the step (1), continuously stirring for 2-2.5 h at the stirring speed of 20-25 rpm/min and the vacuum degree of 0.5-2 Pa, and taking out to obtain the primary honey to be treated;
secondly, a 120-mesh bamboo carbon fiber net is adopted for primary filtration to obtain undoped honey;
thirdly, heating the undoped honey obtained in the second step to 55-60 ℃, keeping the temperature for 15-18 min, and then quickly cooling to 3-5 ℃ to obtain pure honey;
fourthly, the pure honey obtained in the third step is taken out and filled into the glass container prepared in the fifth step of the step 1) to be sealed, and then the required high-activity honey is obtained.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101464461B1 (en) * | 2014-07-31 | 2014-12-04 | 이지근 | Beehive honey picking for Bong Sik beehives Assembly and Western honey bees beekeeping method |
| CN104396892A (en) * | 2014-11-10 | 2015-03-11 | 刘双发 | Double-queen culture method of Chinese bees |
| CN104542490A (en) * | 2013-10-22 | 2015-04-29 | 许应强 | Beekeeping method using mangrove forest flower source |
| CN104756952A (en) * | 2014-01-08 | 2015-07-08 | 罗忠祖 | Longan flower source bee culture method |
| CN107373555A (en) * | 2017-06-22 | 2017-11-24 | 杨佳林 | A kind of natural honey Bee Pollen joint product and preparation method thereof |
| CN107980727A (en) * | 2017-11-27 | 2018-05-04 | 云南中烟工业有限责任公司 | A kind of production method of the special blueberry honey of high-end cigarette |
| CN108077186A (en) * | 2017-11-16 | 2018-05-29 | 宁夏碧蜂源蜂产品有限公司 | A kind of method that more casees efficiently cultivate honey |
-
2019
- 2019-01-09 CN CN201910019415.6A patent/CN109757436B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542490A (en) * | 2013-10-22 | 2015-04-29 | 许应强 | Beekeeping method using mangrove forest flower source |
| CN104756952A (en) * | 2014-01-08 | 2015-07-08 | 罗忠祖 | Longan flower source bee culture method |
| KR101464461B1 (en) * | 2014-07-31 | 2014-12-04 | 이지근 | Beehive honey picking for Bong Sik beehives Assembly and Western honey bees beekeeping method |
| CN104396892A (en) * | 2014-11-10 | 2015-03-11 | 刘双发 | Double-queen culture method of Chinese bees |
| CN107373555A (en) * | 2017-06-22 | 2017-11-24 | 杨佳林 | A kind of natural honey Bee Pollen joint product and preparation method thereof |
| CN108077186A (en) * | 2017-11-16 | 2018-05-29 | 宁夏碧蜂源蜂产品有限公司 | A kind of method that more casees efficiently cultivate honey |
| CN107980727A (en) * | 2017-11-27 | 2018-05-04 | 云南中烟工业有限责任公司 | A kind of production method of the special blueberry honey of high-end cigarette |
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