CN110432262B - Preparation and preservation method of plant cured leaf specimen - Google Patents
Preparation and preservation method of plant cured leaf specimen Download PDFInfo
- Publication number
- CN110432262B CN110432262B CN201910619961.3A CN201910619961A CN110432262B CN 110432262 B CN110432262 B CN 110432262B CN 201910619961 A CN201910619961 A CN 201910619961A CN 110432262 B CN110432262 B CN 110432262B
- Authority
- CN
- China
- Prior art keywords
- plant
- specimen
- color
- extract
- leaf specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 238000004321 preservation Methods 0.000 title abstract description 21
- 241000196324 Embryophyta Species 0.000 claims abstract description 122
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 claims abstract description 27
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000834 fixative Substances 0.000 claims abstract description 26
- 235000002906 tartaric acid Nutrition 0.000 claims abstract description 26
- 239000011975 tartaric acid Substances 0.000 claims abstract description 26
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 claims abstract description 21
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 claims abstract description 21
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 claims abstract description 21
- 229940074393 chlorogenic acid Drugs 0.000 claims abstract description 21
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 claims abstract description 21
- 235000001368 chlorogenic acid Nutrition 0.000 claims abstract description 21
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 claims abstract description 21
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 16
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 16
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 14
- 229920000615 alginic acid Polymers 0.000 claims abstract description 14
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229940072056 alginate Drugs 0.000 claims abstract description 12
- 239000012153 distilled water Substances 0.000 claims abstract description 7
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims abstract description 6
- 238000004108 freeze drying Methods 0.000 claims abstract description 5
- 239000004033 plastic Substances 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims description 31
- 241000628997 Flos Species 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- QBYIENPQHBMVBV-HFEGYEGKSA-N (2R)-2-hydroxy-2-phenylacetic acid Chemical compound O[C@@H](C(O)=O)c1ccccc1.O[C@@H](C(O)=O)c1ccccc1 QBYIENPQHBMVBV-HFEGYEGKSA-N 0.000 claims description 12
- IWYDHOAUDWTVEP-UHFFFAOYSA-N R-2-phenyl-2-hydroxyacetic acid Natural products OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 12
- 229960002510 mandelic acid Drugs 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 7
- 238000009966 trimming Methods 0.000 claims description 6
- CWVRJTMFETXNAD-BMNNCGMMSA-N (1s,3r,4s,5r)-3-[(e)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxy-1,4,5-trihydroxycyclohexane-1-carboxylic acid Chemical compound O[C@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-BMNNCGMMSA-N 0.000 claims description 5
- 239000002390 adhesive tape Substances 0.000 claims description 5
- KRZBCHWVBQOTNZ-DLDRDHNVSA-N isochlorogenic acid Natural products O[C@@H]1[C@H](C[C@@](O)(C[C@H]1OC(=O)C=Cc2ccc(O)c(O)c2)C(=O)O)OC(=O)C=Cc3ccc(O)c(O)c3 KRZBCHWVBQOTNZ-DLDRDHNVSA-N 0.000 claims description 5
- 238000009210 therapy by ultrasound Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 1
- 238000010992 reflux Methods 0.000 claims 1
- KRZBCHWVBQOTNZ-UHFFFAOYSA-N (-) 3,5-dicaffeoyl-muco-quinic acid Natural products OC1C(OC(=O)C=CC=2C=C(O)C(O)=CC=2)CC(O)(C(O)=O)CC1OC(=O)C=CC1=CC=C(O)C(O)=C1 KRZBCHWVBQOTNZ-UHFFFAOYSA-N 0.000 abstract description 15
- KRZBCHWVBQOTNZ-RDJMKVHDSA-M (-)-3,5-Dicaffeoyl quinic acid Natural products O([C@@H]1CC(O)(C[C@H](C1O)OC(=O)\C=C\C=1C=C(O)C(O)=CC=1)C([O-])=O)C(=O)\C=C\C1=CC=C(O)C(O)=C1 KRZBCHWVBQOTNZ-RDJMKVHDSA-M 0.000 abstract description 15
- KRZBCHWVBQOTNZ-PSEXTPKNSA-N 3,5-di-O-caffeoyl quinic acid Chemical compound O([C@@H]1C[C@](O)(C[C@H]([C@@H]1O)OC(=O)\C=C\C=1C=C(O)C(O)=CC=1)C(O)=O)C(=O)\C=C\C1=CC=C(O)C(O)=C1 KRZBCHWVBQOTNZ-PSEXTPKNSA-N 0.000 abstract description 15
- 230000002265 prevention Effects 0.000 abstract description 10
- 238000010415 tidying Methods 0.000 abstract description 5
- 235000015110 jellies Nutrition 0.000 abstract description 3
- 239000008274 jelly Substances 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 18
- 230000000694 effects Effects 0.000 description 16
- 229930014669 anthocyanidin Natural products 0.000 description 12
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 12
- 235000008758 anthocyanidins Nutrition 0.000 description 12
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- 238000003860 storage Methods 0.000 description 6
- XOFYZVNMUHMLCC-ZPOLXVRWSA-N prednisone Chemical compound O=C1C=C[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 XOFYZVNMUHMLCC-ZPOLXVRWSA-N 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- UFCLZKMFXSILNL-RVXRWRFUSA-N 4,5-di-O-caffeoylquinic acid Chemical compound O([C@@H]1C[C@](O)(C[C@H]([C@@H]1OC(=O)\C=C\C=1C=C(O)C(O)=CC=1)O)C(O)=O)C(=O)\C=C\C1=CC=C(O)C(O)=C1 UFCLZKMFXSILNL-RVXRWRFUSA-N 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 230000014759 maintenance of location Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical group CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 241001474374 Blennius Species 0.000 description 3
- 241000201320 Ligustrum japonicum Species 0.000 description 3
- 241000208125 Nicotiana Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 244000234609 Portulaca oleracea Species 0.000 description 3
- 235000001855 Portulaca oleracea Nutrition 0.000 description 3
- 241000219793 Trifolium Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- UFCLZKMFXSILNL-AALYGJCLSA-N 3,4-Dicaffeoylquinic acid Natural products O=C(O[C@@H]1[C@H](OC(=O)/C=C/c2cc(O)c(O)cc2)C[C@](O)(C(=O)O)C[C@@H]1O)/C=C/c1cc(O)c(O)cc1 UFCLZKMFXSILNL-AALYGJCLSA-N 0.000 description 2
- 241000736299 Adiantum Species 0.000 description 2
- 241000037740 Coptis chinensis Species 0.000 description 2
- 241001508399 Elaeagnus Species 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000013475 authorization Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000002274 desiccant Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000411851 herbal medicine Species 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000010409 ironing Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000006911 nucleation Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 238000000643 oven drying Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000013441 quality evaluation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000000859 sublimation Methods 0.000 description 2
- 230000008022 sublimation Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 108091064702 1 family Proteins 0.000 description 1
- GYFFKZTYYAFCTR-JUHZACGLSA-N 4-O-trans-caffeoylquinic acid Chemical compound O[C@@H]1C[C@](O)(C(O)=O)C[C@@H](O)[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 GYFFKZTYYAFCTR-JUHZACGLSA-N 0.000 description 1
- GYFFKZTYYAFCTR-UHFFFAOYSA-N 5-O-(6'-O-galloyl)-beta-D-glucopyranosylgentisic acid Natural products OC1CC(O)(C(O)=O)CC(O)C1OC(=O)C=CC1=CC=C(O)C(O)=C1 GYFFKZTYYAFCTR-UHFFFAOYSA-N 0.000 description 1
- 244000005852 Adiantum capillus veneris Species 0.000 description 1
- 235000013211 Adiantum capillus veneris Nutrition 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 241000218202 Coptis Species 0.000 description 1
- 235000002991 Coptis groenlandica Nutrition 0.000 description 1
- WJHSRFQBVYHKKL-UHFFFAOYSA-N Oroboside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC(O)=C2C(=O)C(C=3C=C(O)C(O)=CC=3)=COC2=C1 WJHSRFQBVYHKKL-UHFFFAOYSA-N 0.000 description 1
- 241000193241 Solanum dulcamara Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- GYFFKZTYYAFCTR-LMRQPLJMSA-N cryptochlorogenic acid Natural products O[C@H]1C[C@@](O)(C[C@H](O)[C@H]1OC(=O)C=Cc2ccc(O)c(O)c2)C(=O)O GYFFKZTYYAFCTR-LMRQPLJMSA-N 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- PEFNSGRTCBGNAN-QNDFHXLGSA-N luteolin 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=C2C(=O)C=C(C=3C=C(O)C(O)=CC=3)OC2=C1 PEFNSGRTCBGNAN-QNDFHXLGSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- GWTUHAXUUFROTF-UHFFFAOYSA-N pseudochlorogenic acid Natural products C1C(O)C(O)C(O)CC1(C(O)=O)OC(=O)C=CC1=CC=C(O)C(O)=C1 GWTUHAXUUFROTF-UHFFFAOYSA-N 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- CWVRJTMFETXNAD-NXLLHMKUSA-N trans-5-O-caffeoyl-D-quinic acid Chemical compound O[C@H]1[C@H](O)C[C@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-NXLLHMKUSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a preparation and preservation method of a plant cured leaf specimen, which belongs to the technical field of plant specimen preparation, and is characterized in that a plant to be prepared into a specimen is trimmed and then is washed clean by distilled water; then adding the extract of honeysuckle, tartaric acid and sea waterAlgin oligose and Al3+The color fixative is used for color fixation treatment, the plant cured leaf specimen is obtained by freeze drying treatment, and finally the cured leaf specimen is sealed in a plastic loose leaf of a file tidying folder and stored according to classification steps. The preparation method uses the content of chlorogenic acid and isochlorogenic acid A in the honeysuckle extract, tartaric acid, alginate jelly oligosaccharide and Al3+The method has the advantages that the method can play a gain role, can keep the original color of the cured leaf specimen, avoids the phenomena of rotting, browning and the like of the cured leaf specimen, perfectly keeps the shape of the cured leaf specimen, and prolongs the time for keeping the original color and shape of the cured leaf specimen; the preservation method can solve the problems of moisture resistance, moth prevention, specimen damage prevention, convenience for classified preservation and the like.
Description
Technical Field
The invention belongs to the technical field of plant specimen preparation, and particularly relates to a preparation and preservation method of a plant cured leaf specimen.
Background
The whole plant or a part thereof is collected and stored for observation at any time, and is called a plant specimen. It is a sample of the finished plant material. If the plant is a medicinal plant, the plant is called a medicinal plant specimen; if the product is preserved after being pressed and dried, the product is called a medicinal plant leaf wax specimen. At present, the method for preparing the plant specimen is the most commonly adopted method for preparing the cured leaf specimen, and the method has the advantages of convenient operation, low cost, good preparation effect and convenient taking in subsequent teaching and research. The most important technology for making the cured leaf specimen is to preserve the fresh plant for a long time after drying the plant on the basis of keeping the original color of the plant as much as possible, and the current drying methods mainly comprise a natural drying method, a drying agent drying method, a microwave drying method, an oven drying method and an ironing drying method. The methods have the advantages and disadvantages that although the natural drying method and the drying agent drying method have the defect of long specimen preparation time, the original color of the specimen is kept better due to simple and convenient operation without additionally carrying multiple tools, and the method is still the most frequently selected method for preparing the existing cured leaf specimen; the microwave drying method and the oven drying method can shorten the preparation time of the specimen, but the operation procedure is more complicated than the former two methods, the microwave oven and the oven are inconvenient to carry, and the specimen preparation power is not higher than the former two methods; although the ironing and drying method can be used for quickly preparing specimens and is suitable for mass preparation of the specimens, the original colors of some plants are not ideal because the temperature in the preparation process is high.
The invention with the authorization bulletin number of CN 103493801B relates to a method for preparing a cured leaf specimen, wherein a fresh plant specimen is directly scalded by Chinese herbal medicine, and the Chinese herbal medicine liquid has strong sterilization, disinsection and mildew prevention effects, can kill worm eggs and spores attached to the surface of a plant, and has a brighter plant color. In addition, the low-temperature air drying treatment can also keep the original color of the plant to prevent the plant from mildewing, and the plant can be stored for a longer time. The invention with the authorization bulletin number of CN 102410948B provides a method for manufacturing a tobacco leaf primary color cured tobacco leaf specimen, which comprises the steps of shaping, fixing color, drying, dampening and the like, the specimen is manufactured more quickly, the time of 6-8 days is shortened compared with that of a conventional pressing method, the color retention effect is good, and the color is kept unchanged in the specimen storage life; the water content is controlled within the range of 8-10%, so that the brittle fracture of the specimen is prevented, and the integrity of the specimen is maintained.
Disclosure of Invention
An object of the present invention is to provide a method for extracting chlorogenic acid and isochlorogenic acid A from flos Lonicerae, and mixing them with tartaric acid, algin oligosaccharide and Al3+The method can play a gain role, can keep the original color of the cured leaf specimen, avoids the phenomena of rotting, browning and the like of the cured leaf specimen, perfectly keeps the shape of the cured leaf specimen, and can keep the original color and shape of the cured leaf specimen for a longer time.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a preparation method of a plant leaf specimen comprises the following steps:
s1: trimming the plant to be made into a specimen and then washing the plant to be made into the specimen by distilled water;
s2: washing the plants with a solution containing flos Lonicerae extract, tartaric acid, alginate-derived oligosaccharide and Al3+Carrying out color protection treatment on the color protection agent;
s3: and (5) carrying out freeze drying treatment on the color-protected plant to obtain the plant cured leaf specimen.
The original color of the cured leaf specimen is difficult to maintain in the preparation process, and the cured leaf specimen is easy to deteriorate, such as blackening, yellowing, whitening and the like. Therefore, how to maintain the wax leaf specimenThe primary color of the product improves the sense of reality of the finished product, and becomes a key technology for making the cured leaf specimen. The anthocyanidin is relatively stable under an acidic condition, the tartaric acid in the color fixative can increase the stability of anthocyanidin, but the solubility of the tartaric acid is reduced at a low temperature, insoluble salt is easy to produce, and the tartaric acid can be separated out in a salt form and cover the surface of a plant in a freeze drying process of mass transfer and heat transfer at a low temperature and a low pressure to form a white powdery solid matter, so that the color fixative cannot play a role. However, the honeysuckle extract in the color fixative used in the invention contains a large amount of chlorogenic acid, isochlorogenic acid, luteoloside and the like, not only has strong sterilization, disinsection and mildew prevention effects, but also can kill ova and spores attached to the surface of the plant, so that the color of the plant is brighter; the anthocyanidin has oxidation resistance, can reduce the sensitivity of the anthocyanidin to oxygen, and can be protected from oxidation, so that the purpose of color protection is achieved. The seaweed gel oligosaccharide in the color fixative can form a layer of protective film on the surface of the plant to block the contact of oxygen and plant tissues, thereby avoiding the oxidation reaction of the anthocyanidin in the plant and the oxygen and playing a role in color protection. Al in color fixative for use in the invention3+Can be combined with anthocyanidin in plant to form chelate with relatively stable chemical and physical properties, thereby protecting color. The invention uses the honeysuckle extract, tartaric acid, algin oligose and Al as the color fixative3+The method has the advantages that the method can play a gain role, reduce the interaction among tartaric acid molecules, improve the solubility of tartaric acid at low temperature, improve the interfacial energy value of the tartaric acid in an aqueous solution, avoid the occurrence of heterogeneous nucleation of the tartaric acid by taking other substances as crystal nuclei, reduce the nucleation rate, avoid the tartaric acid from being separated out in a salt form and then covering the surface of a plant, and further enable the tartaric acid to play a color retention role; in addition, it can also improve the effect of plant cell on flos Lonicerae extract, tartaric acid, alginate-derived oligosaccharide and Al3+The absorption rate of the seaweed gel oligosaccharide and the tartaric acid reduces the chance of cross-linking reaction of the seaweed gel oligosaccharide and the tartaric acid, and improves the color protection effect of the color fixative; finally, the color fixative can destroy the binding force of water and other components in the plant after entering the plant, so that the bound water is dehydratedAnd the drying is favorably and smoothly carried out after the drying is carried out. In conclusion, the color fixative for the invention has excellent effects of sterilization, disinsection, mildew prevention and color protection, can keep the original color of the cured leaf specimen, avoids the phenomena of rotting, browning and the like of the cured leaf specimen, and has longer durability.
The preparation method of the invention also adopts a freeze drying method, so that the water in the plant can form an ice state and be directly sublimated into gaseous water to be volatilized, the structural integrity of the basic skeleton of the plant is ensured, and the shape of the cured leaf specimen is perfectly maintained; the plant is dehydrated completely, and the water content is as low as 2-5%; the anthocyanidin in the plant can avoid the thermal response and ensure the stability of the anthocyanidin, thereby keeping the original color of the cured leaf specimen. The preparation method of the invention not only keeps the original color of the cured leaf specimen and avoids the phenomena of rotting, browning and the like of the cured leaf specimen, but also perfectly keeps the shape of the cured leaf specimen and prolongs the time for keeping the original color and shape of the cured leaf specimen.
Preferably, the color fixative for S2 contains flos Lonicerae extract 5.0-8.0%, tartaric acid 4.8-6.5%, alginate gel oligosaccharide 5.6-8.0%, and Al 0.05-0.1mol/L3+. The color fixative has better color protection, sterilization, disinsection and mildew-proof effects.
Preferably, the content of chlorogenic acid in the honeysuckle extract is at least 180.00mg/g, and the content of isochlorogenic acid in the honeysuckle extract is at least 90.00 mg/g.
Preferably, the S2 is prepared from the honeysuckle extract by the following method:
adding flos Lonicerae powder into alcoholic solution containing mandelic acid, ultrasonic extracting, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain flos Lonicerae extract. The existence of the mandelic acid in the ethanol solution can prevent the chlorogenic acid from being isomerized into new chlorogenic acid and cryptochlorogenic acid, prevent the isochlorogenic acid A from being isomerized into isochlorogenic acid B and isochlorogenic acid C, and improve the content of the chlorogenic acid and the isochlorogenic acid A in the honeysuckle extract. More preferably, the ethanol solution contains 0.5-2% of mandelic acid. Further preferably, the preparation method of the honeysuckle extract comprises the following steps: adding flos Lonicerae powder into 50-70% ethanol solution containing 0.5-2% mandelic acid at a ratio of 1:2-5(g/mL), performing ultrasonic treatment at 40-50 deg.C and power of 100W and 300W for 10-20min, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain flos Lonicerae extract.
Preferably, the pH of the color fixative for S2 is 2.0-3.0. The oxygen atom of one heterocyclic ring on the structure of the anthocyanidin is tetravalent, so that the anthocyanidin shows a basic property, the molecular structure of the anthocyanidin can be changed to a certain degree along with the change of the pH environment, and different color changes are shown, so that the pigment has strong sensitivity to the pH, and the range of the pH between 2.0 and 3.0 can ensure that the anthocyanidin is stable without influencing the original color of plants.
Preferably, the temperature of the color protection treatment in S2 is 40-50 ℃ and the time is 10-60 min. The temperature rise is beneficial to the effective components in the color retention agent to permeate into the interior of the plant, so that a stable color development structure is formed in the interior of the plant, and a good color retention effect is achieved.
Preferably, the specific steps of S3 are:
s31: placing the color-protected plant into a specimen holder, flattening, pre-cooling at-10 deg.C to-5 deg.C for 10-30min, and pre-cooling at-15 deg.C to-10 deg.C for 30-60 min;
s32: drying the pre-cooled plant at-15-10 deg.C under vacuum degree of 5-15Pa for 20-30 hr.
The invention adopts the staged precooling and the processing process of the color fixative, so that water in the plant forms smaller ice crystals, the sublimation drying is easy, the drying process does not influence the skeleton structure of the plant, the shape of the cured leaf specimen is perfectly maintained, and the shape of the cured leaf specimen before and after the plant is dried is maintained.
The invention also discloses a plant leaf specimen prepared by the preparation method.
The invention further discloses a preservation method of the plant leaf specimen, which is characterized in that the prepared leaf specimen is sealed in a plastic loose leaf of a file tidying folder and is stored according to classification steps. The specimen storage device is stored according to classification orders, namely 1 data folder stores 1 classification order, namely 1 plant specimen belonging to 1 genus or 1 family as much as possible, so that the specimen storage device is convenient for searching and taxonomy research, and is convenient to carry and compare the specimens in the field practice process. It can also be classified according to the application, such as flowers, fruit trees, fibers, honey sources, etc. After the specimen is classified, the classified name is pasted on the file folder, preferably on the front and the side simultaneously, so that the storage and the search are convenient, and particularly, some species which are difficult to distinguish are put in a file folder as much as possible, and the difference of the characteristics can be rapidly distinguished in the comparison process. The preservation method can solve the problems of moisture resistance, moth prevention, specimen damage prevention, convenience for classified preservation and the like.
Preferably, the double-sided adhesive tape is used for sealing, and the specimen can be taken out for careful identification when needed.
Compared with the prior art, the invention has the beneficial effects that:
the preparation method uses the content of chlorogenic acid and isochlorogenic acid A in the honeysuckle extract, tartaric acid, alginate jelly oligosaccharide and Al3+The color fixative has the advantages of excellent sterilization, disinsection, mildew resistance and color protection effects, capability of keeping the original color of the cured leaf specimen, prevention of the phenomena of rotting, browning and the like of the cured leaf specimen and longer durability; according to the invention, by adopting stage precooling and matching with the treatment process of the color fixative, water in the plant forms smaller ice crystals, so that sublimation drying is easy, the drying process does not influence the skeleton structure of the plant, the shape of the cured leaf specimen is perfectly maintained, and the shape of the plant before and after drying is maintained; the preparation method of the invention not only keeps the original color of the cured leaf specimen and avoids the cured leaf specimen from rotting, browning and the like, but also perfectly keeps the shape of the cured leaf specimen and prolongs the time for keeping the original color and shape of the cured leaf specimen. The preservation method can solve the problems of moisture resistance, moth prevention, specimen damage prevention, convenience for classified preservation and the like.
The preparation and preservation method for the plant cured leaf specimen provided by the invention overcomes the defects of the prior art, and is reasonable in design and convenient to operate.
Drawings
FIG. 1 is a standard curve of chlorogenic acid and isochlorogenic acid A in test example 1 of the present invention;
FIG. 2 shows the results of measuring the content of chlorogenic acid and isochlorogenic acid A in the flos Lonicerae extract of test example 1;
FIG. 3 shows the measurement results of color difference Δ E of the plant leaf specimen in test example 1 of the present invention.
Detailed Description
The following further describes embodiments of the present invention with reference to specific examples.
Example 1:
a preparation and preservation method of a plant leaf specimen comprises the following steps:
s1: adding flos Lonicerae powder into 50% ethanol solution containing 0.5% mandelic acid at a material-to-liquid ratio of 1:2(g/mL), performing ultrasonic treatment at 40 deg.C with power of 100W for 10min, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain flos Lonicerae extract;
s2: trimming the plant to be made into a specimen and then washing the plant to be made into the specimen by distilled water;
s3: washing the plants with a solution containing 5.0% of S1-derived flos Lonicerae extract, 4.8% of tartaric acid, 5.6% of alginate-derived oligosaccharides and 0.05mol/L of Al3+The color fixative has a pH of 2.0, and is subjected to color fixation treatment at a temperature of 40 ℃ for 10 min;
s4: placing the color-protected plant into a specimen holder, flattening, pre-cooling at-10 deg.C for 10min, and pre-cooling at-15 deg.C for 30 min; and then drying the precooled plant for 20 hours at the vacuum degree of 5Pa and the temperature of-15 ℃ to obtain the plant cured leaf standard.
The preservation method of the plant leaf specimen is characterized in that the prepared leaf specimen is sealed in plastic loose leaves of a file tidying folder by double-faced adhesive tape and is stored according to classification steps.
Example 2:
a preparation and preservation method of a plant leaf specimen comprises the following steps:
s1: adding flos Lonicerae powder into 70% ethanol solution containing 2% mandelic acid at a ratio of 1:5(g/mL), performing ultrasonic treatment at 50 deg.C for 20min at power of 300W, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain flos Lonicerae extract;
s2: trimming the plant to be made into a specimen and then washing the plant to be made into the specimen by distilled water;
s3: washing the plants with a solution containing 8.0% of S1-derived flos Lonicerae extract, 6.5% of tartaric acid, 8.0% of alginate-derived oligosaccharides, and 0.1mol/L Al3+The color fixative has a pH of 3.0, and is subjected to color fixation treatment at a temperature of 50 ℃ for 60 min;
s4: placing the color-protected plant into a specimen holder, flattening, pre-cooling at-5 deg.C for 30min, and pre-cooling at-10 deg.C for 60 min; and then drying the precooled plant for 30 hours at the vacuum degree of 15Pa and the temperature of-10 ℃ to obtain the plant cured leaf standard.
The preservation method of the plant leaf specimen is characterized in that the prepared leaf specimen is sealed in plastic loose leaves of a file tidying folder by double-faced adhesive tape and is stored according to classification steps.
Example 3:
a preparation and preservation method of a plant leaf specimen comprises the following steps:
s1: adding flos Lonicerae powder into 60% ethanol solution containing 1.2% mandelic acid at a material-to-liquid ratio of 1:4(g/mL), performing ultrasonic treatment at 45 deg.C with 200W power for 16min, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain flos Lonicerae extract;
s2: trimming the plant to be made into a specimen and then washing the plant to be made into the specimen by distilled water;
s3: washing the plants with a solution containing 6.0% of S1-derived flos Lonicerae extract, 5.6% of tartaric acid, 7.0% of alginate-derived oligosaccharides, and 0.07mol/L Al3+The color fixative has a pH of 2.5, and is subjected to color fixation treatment at a temperature of 45 ℃ for 30 min;
s4: placing the color-protected plant into a specimen holder, flattening, pre-cooling at-8 deg.C for 20min, and pre-cooling at-12 deg.C for 45 min; and then drying the precooled plants for 24 hours at the vacuum degree of 10Pa and the temperature of-12 ℃ to obtain the plant cured leaf standard.
The preservation method of the plant leaf specimen is characterized in that the prepared leaf specimen is sealed in a file tidying folder by double-faced adhesive tape
Comparative example 1:
a preparation and preservation method of a plant leaf specimen comprises the following steps:
s1: trimming the plant to be made into a specimen and then washing the plant to be made into the specimen by distilled water;
s2: washing the plants with a solution containing 5.6% tartaric acid, 7.0% alginate-derived oligosaccharides and 0.07mol/L Al3+The pH value of the color fixative is 2.5, and color fixation treatment is carried out for 30min at the temperature of 45 ℃;
s4: placing the color-protected plant into a specimen holder, flattening, pre-cooling at-8 deg.C for 20min, and pre-cooling at-12 deg.C for 45 min; and then drying the precooled plants for 24 hours at the vacuum degree of 10Pa and the temperature of-12 ℃ to obtain the plant cured leaf standard.
Comparative example 2:
this comparative example differs from example 3 in that: s1 the ethanol solution does not contain mandelic acid.
Test example 1:
determination of chlorogenic acid and isochlorogenic acid A content in honeysuckle extract
Measuring the content of chlorogenic acid and isochlorogenic acid A in the honeysuckle extract by adopting HPLC;
detection conditions are as follows: eclipse plus C18 column (4.6 mm. times.150 mm, 5 μm); the mobile phase A is 0.1% phosphoric acid buffer solution, and the mobile phase B is acetonitrile; the gradient elution conditions were: 0-20min, 15-30% B; 20-30 min, 30-50% B; the flow rate is 1.0 mL/min; detection wavelength 327 nm; the amount of the sample was 10. mu.L.
Drawing a standard curve of chlorogenic acid: accurately weighing 3.5mg of chlorogenic acid standard and isochlorogenic acid A standard, and diluting to 50mL with 30% methanol solution. Accurately sucking 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.2mL, 1.4 mL, 1.6mL and 1.8mL respectively, diluting to 5mL with 30% methanol solution, mixing well, detecting by HPLC, recording the peak area of wave, taking the mass concentration (C, mu g/mL) as the abscissa X and the peak area (S) as the ordinate Y, plotting, and fitting to obtain the standard curve of chlorogenic acid and isochlorogenic acid A, wherein the result is shown in FIG. 1.
Taking 0.1g of honeysuckle extract, diluting 100 times by adopting 30% methanol solution, detecting the content of chlorogenic acid and isochlorogenic acid A by adopting the same chromatographic condition, carrying out chromatographic analysis, and calculating a standard curve to obtain the content of chlorogenic acid and isochlorogenic acid A, wherein the result is shown in figure 2. It can be seen that the chlorogenic acid content in the honeysuckle extract of examples 1-3 is at least 180.00mg/g, and the isochlorogenic acid content is at least 90.00mg/g, which is much higher than that in comparative example 2, which indicates that the presence of mandelic acid in the ethanol solution can protect chlorogenic acid from being isomerized to form neochlorogenic acid and isochlorogenic acid, protect isochlorogenic acid a from being isomerized to form isochlorogenic acid B and isochlorogenic acid C, and increase the chlorogenic acid and isochlorogenic acid a content in the honeysuckle extract.
Test example 2:
color protection effect of plant leaf specimen
1. Selecting 6 plants of adiantum, clover, ligustrum japonicum, Elaeagnus dulcis, purslane and coptis chinensis, preparing plant leaf specimens according to the methods of examples 1-3 and comparative examples 1-2, measuring the L, a and b values of the prepared leaf specimens by using a color difference meter, comparing the L, a and b values with the L, a and b values of the African chrysanthemum petals to obtain delta L, delta a and delta b, calculating color difference according to the following formula,
the smaller the color difference Δ E, the better the color protection effect is proved. The measurement results of the color difference delta E are shown in FIG. 3, and it can be seen that the color difference delta E of the 6-plant cured tobacco specimens of Adiantum capillus-veneris, Trifolium Pratentis, Ligustrum japonicum, Elaeagnus dulcis, purslane and Coptis chinensis prepared by the examples 1-3 is far smaller than that of the comparative examples 1-2, which shows that the color difference delta E of the honeysuckle flower extract, tartaric acid, alginate jelly oligosaccharide and Al in the color fixative used in the preparation method of the invention3+Can play a role in gaining and enables the color fixative to have excellent color protection effect.
2. Selecting 6 plants of adiantum, clover, ligustrum japonicum, dulcamara, purslane and coptis, preparing plant cured leaf specimens according to the methods of examples 1-3 and comparative examples 1-2, observing the plant state in 1 month, 6 months, 12 months, 18 months and 24 months of storage, and performing quality evaluation, wherein the quality evaluation is divided into 3 grades: preferably (+): the flowers, branches and leaves of the plants still keep the original color and luster after long-term storage, and the shapes of the flowers, branches and leaves are more vivid; good (.: the color of the flowers, branches and leaves of the plants is preserved after a period of time, but the color fading is more obvious and the shape is not very vivid; a difference (-): the original color of the flowers, branches and leaves of the plants is basically lost after the flowers, branches and leaves of the plants are preserved for a period of time, and although the shapes exist, the reality is not realized. The evaluation results are shown in table 1, and it can be seen that,
the preservation effect of the plant leaf specimen in the example 1-2 is better than that in the comparative example 1-2, which shows that the color fixative used in the invention has excellent color fixative effect, can keep the original color of the leaf specimen, and has longer durability.
TABLE 1 preservation Effect of plant leaf specimens
Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.
The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (9)
1. A preparation method of a plant leaf specimen is characterized by comprising the following steps: the method comprises the following steps:
s1: trimming the plant to be made into a specimen and then washing the plant to be made into the specimen by distilled water;
s2: washing the plants with a solution containing flos Lonicerae extract, tartaric acid, alginate-derived oligosaccharide and Al3+The color fixative is subjected to color fixation treatment, and the color fixativeThe composition comprises 5.0-8.0% flos Lonicerae extract, 4.8-6.5% tartaric acid, 5.6-8.0% alginate-derived oligosaccharide, and 0.05-0.1mol/L Al3+The pH value of the color fixative is 2.0-3.0, the content of chlorogenic acid in the honeysuckle extract is at least 180.00mg/g, and the content of isochlorogenic acid is at least 90.00 mg/g;
s3: and (4) carrying out freeze drying treatment on the color-protected plant to obtain the plant cured leaf specimen.
2. The method for preparing a plant leaf specimen according to claim 1, wherein the method comprises the following steps: the S2 is prepared from honeysuckle extract by the following method:
adding flos Lonicerae powder into ethanol solution containing mandelic acid, ultrasonic extracting under reflux, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain flos Lonicerae extract.
3. The method for preparing a plant leaf specimen according to claim 2, wherein the method comprises the following steps: the ethanol solution contains 0.5-2% of mandelic acid.
4. The method for preparing a plant leaf specimen according to claim 1, wherein the method comprises the following steps: the preparation method of the honeysuckle extract comprises the following steps: adding flos Lonicerae powder into 50-70% ethanol solution containing 0.5-2% mandelic acid at a ratio of 1:2-5(g/mL), performing ultrasonic treatment at 40-50 deg.C and power of 100W and 300W for 10-20min, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain flos Lonicerae extract.
5. The method for preparing a plant leaf specimen according to claim 1, wherein the method comprises the following steps: the temperature of the color protection treatment in the S2 is 40-50 ℃, and the time is 10-60 min.
6. The method for preparing a plant leaf specimen according to claim 1, wherein the method comprises the following steps: the specific steps of S3 are as follows:
s31: putting the color-protected plant into a specimen holder, flattening, pre-cooling for 10-30min at the temperature of-10 to-5 ℃, and then pre-cooling for 30-60min at the temperature of-15 to-10 ℃;
s32: and drying the pre-cooled plant for 20-30h at the vacuum degree of 5-15Pa and the temperature of-15 to-10 ℃.
7. A plant leaf specimen prepared by the method of any one of claims 1 to 6.
8. The plant leaf specimen of claim 7, wherein: and sealing the prepared wax leaf specimen in a plastic loose leaf of a file sorting folder, and storing according to classification steps.
9. The plant leaf specimen according to claim 8, wherein: the sealing and storing adopts double-sided adhesive tape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910619961.3A CN110432262B (en) | 2019-07-10 | 2019-07-10 | Preparation and preservation method of plant cured leaf specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910619961.3A CN110432262B (en) | 2019-07-10 | 2019-07-10 | Preparation and preservation method of plant cured leaf specimen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110432262A CN110432262A (en) | 2019-11-12 |
| CN110432262B true CN110432262B (en) | 2021-06-08 |
Family
ID=68430068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910619961.3A Active CN110432262B (en) | 2019-07-10 | 2019-07-10 | Preparation and preservation method of plant cured leaf specimen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110432262B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115530158A (en) * | 2022-10-27 | 2022-12-30 | 濮阳市食品药品检验检测中心 | Method for making plant specimen with high-efficiency color retention |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885030B (en) * | 2012-10-29 | 2014-04-09 | 广州栋方日化有限公司 | Method for maintaining stability of rose petals in faintly-acid or neutral cosmetic system |
| CN103493801B (en) * | 2013-09-18 | 2015-03-11 | 安徽师范大学 | Method for preparing herbarium specimens |
| CN107736344A (en) * | 2017-11-14 | 2018-02-27 | 四川农业大学 | A kind of cotton rose protects color drying process |
| CN108102003A (en) * | 2017-12-26 | 2018-06-01 | 浦江县美泽生物科技有限公司 | The preparation method of matrimony vine refined polysaccharide with antioxidation activity |
| CN108514123A (en) * | 2018-02-28 | 2018-09-11 | 磐安县派普特生物科技有限公司 | The active peptides extracted from blood clam prepare compound method |
-
2019
- 2019-07-10 CN CN201910619961.3A patent/CN110432262B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110432262A (en) | 2019-11-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gaff | Desiccation tolerant vascular plants of Southern Africa | |
| Hosokawa et al. | On the osmotic pressure and resistance to desiccation of epiphytic mosses from a beech forest, south-west Japan | |
| Schonbeck et al. | Responses of the moss Tortula ruralis to desiccation treatments. II. Variations in desiccation tolerance | |
| Villiers et al. | Dormancy in Fruits of Fraxinus excelsior L. | |
| Sobin et al. | The isolation and absorption spectrum maxima of bacterial carotenoid pigments | |
| CN110432262B (en) | Preparation and preservation method of plant cured leaf specimen | |
| CN103609439B (en) | Different strain is on the impact of ginseng Hairy root and application method | |
| Thirumalachar et al. | Notes on some mycological methods | |
| Kumar et al. | Drying of flowers: A money-spinning aspect of floriculture industry | |
| Newton | The nature and practical measurement of frost resistance in winter wheat | |
| CN112602705A (en) | Processing method for restoring and color-preserving plant specimen | |
| Bala et al. | Practicals in plant physiology and biochemistry | |
| Darwin et al. | Practical physiology of plants | |
| Di Nola et al. | Respiration, photosynthesis and drought tolerance in mosses from various habitats in Israel | |
| CN111035668A (en) | Preparation method and application of instant isatis tinctoria leaf extract | |
| Jain et al. | Storage of pollen grains in organic solvents: effects of solvents on pollen viability and membrane integrity | |
| US3440317A (en) | Cell coloring process and composition for cytological examination | |
| Uphaus et al. | Effects of heavy water on Atropa belladonna | |
| Parry | Acclimatisation in the green spruce aphid, Elatobium abietinum | |
| NL2030772B1 (en) | Method for promoting germination of seed of gentianaceae after storage | |
| Liu et al. | The measurement of net photosynthesis of three species of Plagiomnium mosses and its relation to the light and temperature | |
| Sekhara Varma et al. | Biosynthesis of citric acid in citrus fruits | |
| Cejas et al. | Impact of liquid nitrogen exposure on selected biochemical and structural parameters of hydrated Phaseolus vulgaris L. seeds | |
| CN112931492A (en) | Crude extract of lonicera japonica fruit plant material ionized by strong acid H + ions and application of crude extract | |
| JPS6321472B2 (en) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |