CN110432262A - A kind of preparation of waxed plant leaves sample and store method - Google Patents
A kind of preparation of waxed plant leaves sample and store method Download PDFInfo
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- CN110432262A CN110432262A CN201910619961.3A CN201910619961A CN110432262A CN 110432262 A CN110432262 A CN 110432262A CN 201910619961 A CN201910619961 A CN 201910619961A CN 110432262 A CN110432262 A CN 110432262A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The present invention provides preparation and the store method of a kind of waxed plant leaves sample, belongs to plant specimen manufacture technology field, will be clean with distilled water flushing after the vegetation pruning of sample to be produced；It uses again and contains Honegsukle flower P.E, tartaric acid, seaweed glue oligosaccharide and Al3+Color stabilizer carry out color protection treatment, freeze-drying process obtains waxed plant leaves sample, is finally sealed in exsiccata in the plastics loose-leaf of file consolidation folder, is stored by taxonomic category.The content of preparation method of the present invention Honegsukle flower P.E Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid, with tartaric acid, seaweed glue oligosaccharide and Al3+Phenomena such as gain effect can be played, exsiccata can be made to keep original color, avoid rotten exsiccata generation, brown stain, and the reservation for making the shape of exsiccata improve, and the time for making exsiccata keep original color and shape is longer；Store method of the present invention can solve it is moisture-proof, mothproof, prevent that sample is damaged, the problems such as saving convenient for classification.
Description
Technical field
The invention belongs to plant specimen manufacture technology fields, and in particular to a kind of preparation of waxed plant leaves sample and preservation side
Method.
Background technique
Whole plant or in which a part are saved after acquisition, in case observe at any time, referred to as plant specimen.
It is the plant sample by arrangement.If plant is medicinal plant, it is called medicinal plant sample；If through over-voltage
It is saved after system is dry, is then called medicinal plant exsiccata.Currently, plant specimen it is on the make commonly used be cured
Leaves specimen method, the method is easy to operate, at low cost, and production effect is good and takes in Following course and research conveniently.Cured leaf
Preparation of specimen's technology will most importantly protect for a long time on the basis of keeping plant original color as far as possible after fresh plant drying
It deposits, current drying method mainly has natural seasoning, desiccant dryness method, micro-wave drying method, oven method, ironing drying
Method.These methods cut both ways, although natural seasoning, desiccant dryness method have preparation of specimen's time longer deficiency, but
It due to easy to operate, is not required to additionally carry excessive tool, it is still current exsiccata production that sample original color, which is kept preferably,
The method most often selected；Micro-wave drying method, oven method can shorten the Production Time of sample, but operation sequence is earlier above
Two methods are cumbersome, and micro-wave oven and baking oven is inconvenient to carry and preparation of specimen's success rate does not have first two method high；Ironing is dry
Though sample can be quickly made in dry method, certain because manufacturing process temperature is higher suitable for a large amount of production of sample
Plant original color keeps undesirable.
Authorization Notice No. is that the invention of 103493801 B of CN is related to a kind of exsiccata production method, directly utilizes medium-height grass
Medicine scalding fresh plant sample, the Chinese herbal medicine fluid have very strong sterilization and disinsection and mildew-proof function, can kill and be attached to plant
The worm's ovum and spore on surface, vegetation color are also vivider.In addition low-temperature air-drying processing can also make plant that primary colors be kept to prevent mould
Become, it is more permanent to save.Authorization Notice No. provides a kind of tobacco leaf primary color exsiccata for the invention of 102410948 B of CN
Production method, including sizing, fixation, drying, resurgence, production sample more rapidly, than conventional pressing shorten 6~8
It time, guarantor's color effect is good, and within the Saving specimen phase, color keep is constant；Water content controls in 8%~10% range,
Sample embrittlement is prevented, the integrality of sample is maintained.
Summary of the invention
It is an object of the present invention to provide containing for a kind of Honegsukle flower P.E Content of Chlorogenic Acid used and 3,5-Dicaffeoylquinic acid
Amount, with tartaric acid, seaweed glue oligosaccharide and Al3+Gain effect can be played, exsiccata can be made to keep original color, avoided
Phenomena such as exsiccata occurs to rot, brown stain, and the reservation for making the shape of exsiccata improve, and make exsiccata
Keep the preparation method of the time of original color and shape longer waxed plant leaves sample.
The technical solution that the present invention is taken to achieve the above object are as follows:
A kind of preparation method of waxed plant leaves sample, comprising the following steps:
S1: will be clean with distilled water flushing after the vegetation pruning of sample to be produced；
S2: plant after flushing is used and contains Honegsukle flower P.E, tartaric acid, seaweed glue oligosaccharide and Al3+Color stabilizer into
Row color protection treatment；
S3: plant after color protection is subjected to freeze-drying process to get waxed plant leaves sample.
Exsiccata is produced on primary colors in manufacturing process and is difficult to be kept, and easily blacks, turns to be yellow, the iso-metamorphism that whitens shows
As.Therefore, the primary colors for how keeping exsiccata improves the sense of reality of finished product, it has also become the key of people's production exsiccata
Technology.Anthocyanidin is more stable in acid condition, and the tartaric acid in present invention color stabilizer can increase the stabilization of anthocyanidin
Property, but tartaric acid solubility in low temperature can reduce, and be readily produced insoluble salt, in the mass-and heat-transfer of low-temp low-pressure
In freezing dry process, tartaric acid can be precipitated in a salt form, be covered on plant surface, form white powdery solid substance, no
It can play the role of color protection.But contain a large amount of chlorogenic acid and different green in the Honegsukle flower P.E in present invention color stabilizer
Ortho acid and galuteolin etc. not only have very strong sterilization and disinsection and mildew-proof function, can kill and be attached to plant surface
Worm's ovum and spore so that vegetation color is also vivider；But also there is inoxidizability, it can reduce anthocyanidin to oxygen
Sensitivity, protection anthocyanidin it is not oxidized, to achieve the purpose that color protection.Seaweed glue in present invention color stabilizer is low
Glycan can form layer protecting film in plant surface, contact of the oxygen with plant tissue be hindered, to avoid the pattern in plant
Contact of the element with oxygen and oxidation reaction occurs, play color-protecting function.Al in present invention color stabilizer3+Can in plant
Anthocyanidin combines, and chemical property and the metastable chelate of physical property is formed, to play the role of color protection.This hair
Bright Honegsukle flower P.E, tartaric acid, seaweed glue oligosaccharide and Al in color stabilizer3+Gain effect can be played, tartaric acid point is reduced
Interaction between son improves tartaric acid solubility in low temperature, and tartaric acid also can be improved, and interface can be worth in aqueous solution, keep away
Exempt from tartaric acid and nonhomogen-ous nucleation occurs using other substances as nucleus, reduces nucleation rate, avoid tartaric acid can be in a salt form
It is covered on plant surface after precipitation, protects color effect so as to make tartaric acid play it；Moreover it is possible to improve plant cell to gold
Honeysuckle flower extract, tartaric acid, seaweed glue oligosaccharide and Al3+Absorption rate, reduce seaweed glue oligosaccharide and tartaric acid and hand over
The chance for joining reaction, improves the effect of color protection of color stabilizer；Finally, color stabilizer can destroy water and plant after entering inside plants
The binding force of interior other compositions is gone on smoothly so that detaching in conjunction with water conducive to dry.To sum up, present invention color stabilizer
With excellent sterilization and disinsection and mould proof and color-protecting function, exsiccata can be made to keep original color, avoid exsiccata
Occur to rot, brown stain phenomena such as, and persistence is longer.
Preparation method of the present invention also takes freeze-drying method, and endophytic moisture can be made to form ice body state and directly rise
China becomes vaporous water and volatilizees away, guarantees the basic skeleton structure integrality of plant, the guarantor that the shape of exsiccata improves
It stays；More thoroughly, moisture content is down to 2%-5% for plant dehydration ratio；Anthocyanidin in plant can be avoided thermal sensitivity reaction, can
Guarantee the stability of anthocyanidin, so that exsiccata be made to keep original color.Preparation method of the present invention not only makes exsiccata
Phenomena such as keeping original color, avoiding rotten exsiccata generation, brown stain, and the shape of exsiccata is made to improve
Reservation, and the time for making exsiccata keep original color and shape is longer.
Preferably, 5.0-8.0% Honegsukle flower P.E, 4.8-6.5% tartaric acid, 5.6- are contained in S2 color stabilizer
8.0% seaweed glue oligosaccharide and 0.05-0.1mol/L Al3+.The color protection and sterilization and disinsection and anti-mold effect of the color stabilizer are more
It is good.
Preferably, the content of Honegsukle flower P.E Content of Chlorogenic Acid is at least 180.00mg/g, and the content of isochlorogenic acid is at least
For 90.00mg/g.
Preferably, S2 is made by the following method with Honegsukle flower P.E:
Honeysuckle Flower is added in the ethanol solution containing mandelic acid, ultrasonic extraction, filters, be concentrated under reduced pressure into medicinal extract,
Drying is to get Honegsukle flower P.E.The presence of mandelic acid can protect chlorogenic acid not to be isomerized to be formed newly in ethanol solution
Chlorogenic acid and Cryptochlorogenic acid protect 3,5-Dicaffeoylquinic acid not to be isomerized to form 3,4-Dicaffeoylquinic acid and 4,5-Dicaffeoylquinic acid, improve honeysuckle
The content of extract Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid.More preferably, containing the mandelic acid of 0.5-2% in ethanol solution.Into one
Preferably, honeysuckle flower extract contains 0.5-2% the preparation method comprises the following steps: Honeysuckle Flower is added for 1:2-5 (g/mL) by solid-liquid ratio to step
Mandelic acid 50-70% ethanol solution in, through power be 100-300W, temperature is 40-50 DEG C of ultrasonic treatment 10-
20min is filtered, and is concentrated under reduced pressure into medicinal extract, dry to get Honegsukle flower P.E.
Preferably, the pH of S2 color stabilizer is 2.0-3.0.The oxygen atom of one of heterocycle is four on structural anthocyanin
Valence makes anthocyanidin itself show a kind of alkalinity, so the molecular structure of anthocyanidin can also occur with the variation of pH environment
Certain variation, and different color changes is showed, so pigment has stronger sensibility to pH, and pH is 2.0-3.0's
Range can make anthocyanidin more stable, not influence the color of plant script.
Preferably, the temperature of color protection treatment is 40-50 DEG C in S2, time 10-60min.The raising of temperature is conducive to protect
Each effective component penetrates into inside plants in toner, and inside plants is made to form stable colour developing structure, reaches good guarantor's color
Effect.
Preferably, the specific steps of S3 are as follows:
S31: plant after color protection being put into specimen holder and is flattened, and 10-30min is pre-chilled at being -10 to -5 DEG C in temperature, then
30-60min is pre-chilled at being -15 to -10 DEG C in temperature；
S32: plant after pre-cooling is dried into 20-30h in the case where vacuum degree is 5-15Pa, temperature is -15 to -10 DEG C.
The present invention takes to be pre-chilled stage by stage, cooperates the treatment process of color stabilizer of the invention, make in plant moisture formation compared with
Small ice crystal is easy to lyophilization, and dry process does not influence the skeleton structure of plant, obtains the shape of exsiccata
To perfect reservation, shape before and after plant drying is maintained.
Invention additionally discloses waxed plant leaves samples made from a kind of above-mentioned preparation method.
A kind of store method of above-mentioned waxed plant leaves sample is further disclosed in the present invention, and the exsiccata made is sealed
It is stored in the plastics loose-leaf of file consolidation folder, is stored by taxonomic category.It is stored by taxonomic category, that is, 1 money
Feed collet stores the plant specimen of 1 taxonomic category, i.e., 1 category or 1 section as far as possible, in this way convenient for lookup and means of taxonomic research, and
And comparison that is easy to carry during field trip and carrying out sample.It can also classify by application, such as flowers class, fruit
Set class, fiber-like, nectar source class etc..The title of classification is posted after specimen classification on information kit, preferably in front and side
It posts, convenient for storage and searches, especially some nondescript species are placed in a file as far as possible, are comparing simultaneously
In the process can fast resolution go out the difference of its feature.Store method of the present invention can solve it is moisture-proof, mothproof, prevent sample damaged, just
In the problems such as saving of classifying.
Preferably, it seals up for safekeeping using double-sided adhesive, sample can also be taken out when needing and carefully be identified.
Compared with prior art, the invention has the benefit that
The content of preparation method of the present invention Honegsukle flower P.E Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid, with tartaric acid, seaweed glue
Oligosaccharide and Al3+Gain effect can be played, so that color stabilizer has excellent sterilization and disinsection and mould proof and color-protecting function, it can
Phenomena such as making exsiccata keep original color, avoiding rotten exsiccata generation, brown stain, and persistence is longer；The present invention
It takes and is pre-chilled stage by stage, cooperate the treatment process of color stabilizer of the invention, so that moisture in plant is formed lesser ice crystal, easily
In lyophilization, and dry process does not influence the skeleton structure of plant, the reservation for making the shape of exsiccata improve,
Maintain plant drying front and back shape；Preparation method of the present invention not only makes exsiccata keep original color, avoids exsiccata
Occur to rot, brown stain phenomena such as, and the reservation for making the shape of exsiccata improve, and exsiccata is made to keep original
Color and shape time it is longer.Store method of the present invention can solve it is moisture-proof, mothproof, prevent that sample is damaged, protects convenient for classification
The problems such as depositing.
Present invention employs above-mentioned technical proposals to provide preparation and the store method of a kind of waxed plant leaves sample, compensates for existing
There are the deficiency of technology, reasonable design, easy operation.
Detailed description of the invention
Fig. 1 is the standard curve of test example 1 Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid of the present invention；
Fig. 2 is Honegsukle flower P.E Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid assay result in test example 1 of the present invention；
Fig. 3 is the color difference △ E measurement result of waxed plant leaves sample in test example 1 of the present invention.
Specific embodiment
In the following, being described further in conjunction with specific embodiments to embodiment of the present invention.
Embodiment 1:
A kind of preparation of waxed plant leaves sample and store method, comprising the following steps:
S1: Honeysuckle Flower is added to 50% ethanol solution of the mandelic acid containing 0.5% by solid-liquid ratio for 1:2 (g/mL)
In, through power be 100W, temperature is 40 DEG C of ultrasonic treatment 10min, is filtered, and medicinal extract is concentrated under reduced pressure into, dry to get honeysuckle
Extract；
S2: will be clean with distilled water flushing after the vegetation pruning of sample to be produced；
S3: plant after flushing is low with Honegsukle flower P.E, 4.8% tartaric acid, 5.6% seaweed glue is obtained containing 5.0%S1
Glycan and 0.05mol/L Al3+Color stabilizer, the pH of color stabilizer is 2.0, carries out color protection treatment 10min at being 40 DEG C in temperature；
S4: plant after color protection being put into specimen holder and is flattened, and 10min is pre-chilled at being -10 DEG C in temperature, then in temperature
It is that 30min is pre-chilled at -15 DEG C；Then plant after pre-cooling is dried into 20h in the case where vacuum degree is 5Pa, temperature is -15 DEG C to get plant
Object exsiccata.
The exsiccata made is sealed in file using double-sided adhesive by a kind of store method of above-mentioned waxed plant leaves sample
In the plastics loose-leaf of sorting clip, stored by taxonomic category.
Embodiment 2:
A kind of preparation of waxed plant leaves sample and store method, comprising the following steps:
S1: Honeysuckle Flower is added to 70% ethanol solution of the mandelic acid containing 2% by solid-liquid ratio for 1:5 (g/mL)
In, through power be 300W, temperature is 50 DEG C of ultrasonic treatment 20min, is filtered, and medicinal extract is concentrated under reduced pressure into, dry to get honeysuckle
Extract；
S2: will be clean with distilled water flushing after the vegetation pruning of sample to be produced；
S3: plant after flushing is low with Honegsukle flower P.E, 6.5% tartaric acid, 8.0% seaweed glue is obtained containing 8.0%S1
Glycan and 0.1mol/L Al3+Color stabilizer, the pH of color stabilizer is 3.0, carries out color protection treatment 60min at being 50 DEG C in temperature；
S4: plant after color protection being put into specimen holder and is flattened, temperature be -5 DEG C at 30min is pre-chilled, then temperature be -
60min is pre-chilled at 10 DEG C；Then plant after pre-cooling is dried into 30h in the case where vacuum degree is 15Pa, temperature is -10 DEG C to get plant
Exsiccata.
The exsiccata made is sealed in file using double-sided adhesive by a kind of store method of above-mentioned waxed plant leaves sample
In the plastics loose-leaf of sorting clip, stored by taxonomic category.
Embodiment 3:
A kind of preparation of waxed plant leaves sample and store method, comprising the following steps:
S1: Honeysuckle Flower is added to 60% ethanol solution of the mandelic acid containing 1.2% by solid-liquid ratio for 1:4 (g/mL)
In, through power be 200W, temperature is 45 DEG C of ultrasonic treatment 16min, is filtered, and medicinal extract is concentrated under reduced pressure into, dry to get honeysuckle
Extract；
S2: will be clean with distilled water flushing after the vegetation pruning of sample to be produced；
S3: plant after flushing is low with Honegsukle flower P.E, 5.6% tartaric acid, 7.0% seaweed glue is obtained containing 6.0%S1
Glycan and 0.07mol/L Al3+Color stabilizer, the pH of color stabilizer is 2.5, carries out color protection treatment 30min at being 45 DEG C in temperature；
S4: plant after color protection being put into specimen holder and is flattened, temperature be -8 DEG C at 20min is pre-chilled, then temperature be -
45min is pre-chilled at 12 DEG C；Then plant after pre-cooling is dried in the case where vacuum degree is 10Pa, temperature is -12 DEG C for 24 hours to get plant
Exsiccata.
The exsiccata made is sealed in file using double-sided adhesive by a kind of store method of above-mentioned waxed plant leaves sample
Sorting clip
Comparative example 1:
A kind of preparation of waxed plant leaves sample and store method, comprising the following steps:
S1: will be clean with distilled water flushing after the vegetation pruning of sample to be produced；
S2: plant after flushing is used and contains 5.6% tartaric acid, 7.0% seaweed glue oligosaccharide and 0.07mol/L Al3+Shield
Toner, the pH of color stabilizer are 2.5, carry out color protection treatment 30min at being 45 DEG C in temperature；
S4: plant after color protection being put into specimen holder and is flattened, temperature be -8 DEG C at 20min is pre-chilled, then temperature be -
45min is pre-chilled at 12 DEG C；Then plant after pre-cooling is dried in the case where vacuum degree is 10Pa, temperature is -12 DEG C for 24 hours to get plant
Exsiccata.
Comparative example 2:
Compared with Example 3, this comparative example the difference is that: in S1 ethanol solution be free of mandelic acid.
Test example 1:
The measurement of Honegsukle flower P.E Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid content
Honegsukle flower P.E Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid content are measured using HPLC；
Testing conditions: Eclipse plus C18 chromatographic column (4.6mm × 150mm, 5 μm)；Mobile phase A is 0.1% phosphoric acid
Buffer, Mobile phase B are acetonitrile；Condition of gradient elution are as follows: 0-20min, 15-30%B；20-30min, 30-50%B；Stream
Fast 1.0mL/min；Detection wavelength 327nm；10 μ L of sample volume.
The drafting of chlorogenic acid standard curve: accurately weighing chlorogenic acid standard items, 3,5-Dicaffeoylquinic acid standard items 3.5mg respectively, uses
30% methanol solution is settled to 50mL.It is accurate respectively to draw 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL, 1.2mL, 1.4
ML, 1.6mL, 1.8mL are settled to 5mL with 30% methanol solution, mix well, and HPLC detection records PeakArea, with quality
Concentration (C, μ g/mL) is abscissa X, with peak area (S) for ordinate Y, is drawn, and fitting obtains chlorogenic acid and 3,5-Dicaffeoylquinic acid
Standard curve, as a result as shown in Figure 1.
0.1g Honegsukle flower P.E is taken, after diluting 100 times using 30% methanol solution, is detected using same chromatographic condition
Chlorogenic acid and 3,5-Dicaffeoylquinic acid content carry out chromatography, chlorogenic acid and 3,5-Dicaffeoylquinic acid content are calculated in standard curve,
As a result as shown in Figure 2.As can be seen that the content of embodiment 1-3 Honegsukle flower P.E Content of Chlorogenic Acid is at least 180.00mg/g,
The content of isochlorogenic acid is at least 90.00mg/g, significantly larger than comparative example 2, this illustrates the presence of mandelic acid in ethanol solution
Chlorogenic acid can be protected not to be isomerized to form neochlorogenic acid and isochlorogenic acid, protect 3,5-Dicaffeoylquinic acid be not isomerized to be formed it is different
Chlorogenic acid B and 4,5-Dicaffeoylquinic acid improve the content of Honegsukle flower P.E Content of Chlorogenic Acid and 3,5-Dicaffeoylquinic acid.
Test example 2:
The effect of color protection of waxed plant leaves sample
1. venushair fern, clover, ligustrum, Elaeocarpus decipiens, 6 kinds of purslane, coptis plants are selected, respectively according to embodiment 1-
3, comparative example 1-2 method prepares waxed plant leaves sample, L, a, b value of made exsiccata is then measured with color difference meter, with Africa
L, a, b value of daisy_petal part compare, and obtain △ L, △ a, △ b, calculate color difference according to the following formula,Color difference △ E is smaller, it was demonstrated that effect of color protection is better.Measurement result such as Fig. 3 institute of color difference △ E
Show, it can be seen that the 6 kinds of plants of venushair fern, clover, ligustrum, Elaeocarpus decipiens, purslane, the coptis prepared through embodiment 1-3 are cured
The color difference △ E of leaves specimen is far smaller than comparative example 1-2, this illustrate Honegsukle flower P.E in preparation method color stabilizer of the present invention,
Tartaric acid, seaweed glue oligosaccharide and Al3+Gain effect can be played, makes color stabilizer that there is excellent effect of color protection.
2. venushair fern, clover, ligustrum, Elaeocarpus decipiens, 6 kinds of purslane, coptis plants are selected, respectively according to embodiment 1-
3, comparative example 1-2 method prepared waxed plant leaves sample, respectively at 1 month, 6 months, 12 months, 18 months, 24 of storage
Month, Plant state is observed, quality evaluation is carried out, quality evaluation is divided into 3 grades: excellent (+): the flower or branches and leaves of plant are through too long
The preservation of phase still keeps original color, and form is also more true to nature；Good (*): the flower or branches and leaves of plant are through guarantor after a period of time
Color is deposited to remain, but it is more obvious to fade, form is less true to nature；Poor (-): the flower or branches and leaves of plant are through guarantor after a period of time
It deposits original color to lose substantially, although form is in the presence of without the sense of reality.Evaluation result such as table 1, it can be seen that
The preservation effect of the preservation effect of embodiment 1-2 waxed plant leaves sample is better than comparative example 1-2, this illustrates use of the present invention
Color stabilizer has excellent color-protecting function, exsiccata can be made to keep original color, and persistence is longer.
The preservation effect of 1 waxed plant leaves sample of table
The prior art of routine techniques dawn known to those skilled in the art in above-described embodiment, therefore herein no longer in detail
It repeats.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field
Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent
Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.
Claims (10)
1. a kind of preparation method of waxed plant leaves sample, it is characterised in that: the following steps are included:
S1: will be clean with distilled water flushing after the vegetation pruning of sample to be produced；
S2: plant after the flushing is used and contains Honegsukle flower P.E, tartaric acid, seaweed glue oligosaccharide and Al3+Color stabilizer into
Row color protection treatment；
S3: plant after the color protection is subjected to freeze-drying process to get waxed plant leaves sample.
2. a kind of preparation method of waxed plant leaves sample according to claim 1, it is characterised in that: the S2 color stabilizer
In contain 5.0-8.0% Honegsukle flower P.E, 4.8-6.5% tartaric acid, 5.6-8.0% seaweed glue oligosaccharide and 0.05-
0.1mol/L Al3+。
3. a kind of preparation method of waxed plant leaves sample according to claim 1 or 2, it is characterised in that: the honeysuckle
The content of extract Content of Chlorogenic Acid is at least 180.00mg/g, and the content of isochlorogenic acid is at least 90.00mg/g.
4. a kind of preparation method of waxed plant leaves sample according to claim 1 or 2, it is characterised in that: the S2 gold
Honeysuckle flower extract is made by the following method:
Honeysuckle Flower is added in the ethanol solution containing mandelic acid, ultrasound, refluxing extraction filter, are concentrated under reduced pressure into medicinal extract,
Drying is to get Honegsukle flower P.E.
5. a kind of preparation method of waxed plant leaves sample according to claim 1 or 2, it is characterised in that: the S2 shield
The pH of toner is 2.0-3.0.
6. a kind of preparation method of waxed plant leaves sample according to claim 1 or 2, it is characterised in that: protected in the S2
The temperature of color processing is 40-50 DEG C, time 10-60min.
7. a kind of preparation method of waxed plant leaves sample according to claim 1, it is characterised in that: the specific step of the S3
Suddenly are as follows:
S31: plant after the color protection being put into specimen holder and is flattened, and 10-30min is pre-chilled at being -10 to -5 DEG C in temperature, then
30-60min is pre-chilled at being -15 to -10 DEG C in temperature；
S32: plant after the pre-cooling is dried into 20-30h in the case where vacuum degree is 5-15Pa, temperature is -15 to -10 DEG C.
8. waxed plant leaves sample made from a kind of described in any item preparation methods of claim 1-7.
9. a kind of store method of waxed plant leaves sample according to any one of claims 8, it is characterised in that: the exsiccata that will be made
It is sealed in the plastics loose-leaf of file consolidation folder, is stored by taxonomic category.
10. a kind of store method of waxed plant leaves sample according to claim 1, it is characterised in that: described seal up for safekeeping is adopted
Use double-sided adhesive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910619961.3A CN110432262B (en) | 2019-07-10 | 2019-07-10 | Preparation and preservation method of plant cured leaf specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910619961.3A CN110432262B (en) | 2019-07-10 | 2019-07-10 | Preparation and preservation method of plant cured leaf specimen |
Publications (2)
| Publication Number | Publication Date |
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| CN110432262A true CN110432262A (en) | 2019-11-12 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115530158A (en) * | 2022-10-27 | 2022-12-30 | 濮阳市食品药品检验检测中心 | Method for making plant specimen with high-efficiency color retention |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885030A (en) * | 2012-10-29 | 2013-01-23 | 广州栋方日化有限公司 | Method for maintaining stability of rose petals in faintly-acid or neutral cosmetic system |
| CN103493801A (en) * | 2013-09-18 | 2014-01-08 | 安徽师范大学 | Method for preparing herbarium specimens |
| CN107736344A (en) * | 2017-11-14 | 2018-02-27 | 四川农业大学 | A kind of cotton rose protects color drying process |
| CN108102003A (en) * | 2017-12-26 | 2018-06-01 | 浦江县美泽生物科技有限公司 | The preparation method of matrimony vine refined polysaccharide with antioxidation activity |
| CN108514123A (en) * | 2018-02-28 | 2018-09-11 | 磐安县派普特生物科技有限公司 | The active peptides extracted from blood clam prepare compound method |
-
2019
- 2019-07-10 CN CN201910619961.3A patent/CN110432262B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102885030A (en) * | 2012-10-29 | 2013-01-23 | 广州栋方日化有限公司 | Method for maintaining stability of rose petals in faintly-acid or neutral cosmetic system |
| CN103493801A (en) * | 2013-09-18 | 2014-01-08 | 安徽师范大学 | Method for preparing herbarium specimens |
| CN107736344A (en) * | 2017-11-14 | 2018-02-27 | 四川农业大学 | A kind of cotton rose protects color drying process |
| CN108102003A (en) * | 2017-12-26 | 2018-06-01 | 浦江县美泽生物科技有限公司 | The preparation method of matrimony vine refined polysaccharide with antioxidation activity |
| CN108514123A (en) * | 2018-02-28 | 2018-09-11 | 磐安县派普特生物科技有限公司 | The active peptides extracted from blood clam prepare compound method |
Non-Patent Citations (1)
| Title |
|---|
| 马玉心 等: "一种简单实用的植物腊叶标本保存技术", 《生物学通报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115530158A (en) * | 2022-10-27 | 2022-12-30 | 濮阳市食品药品检验检测中心 | Method for making plant specimen with high-efficiency color retention |
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