CN117999482A - 用于测定信号扩增的方法、组合物和试剂盒 - Google Patents
用于测定信号扩增的方法、组合物和试剂盒 Download PDFInfo
- Publication number
- CN117999482A CN117999482A CN202280051025.7A CN202280051025A CN117999482A CN 117999482 A CN117999482 A CN 117999482A CN 202280051025 A CN202280051025 A CN 202280051025A CN 117999482 A CN117999482 A CN 117999482A
- Authority
- CN
- China
- Prior art keywords
- detectable label
- reagent
- detectable
- label
- signal amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
- C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
- C07F15/0046—Ruthenium compounds
- C07F15/0053—Ruthenium compounds without a metal-carbon linkage
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163215660P | 2021-06-28 | 2021-06-28 | |
| US63/215,660 | 2021-06-28 | ||
| PCT/US2022/035068 WO2023278293A1 (en) | 2021-06-28 | 2022-06-27 | Methods, compositions, and kits for assay signal amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117999482A true CN117999482A (zh) | 2024-05-07 |
Family
ID=82703171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280051025.7A Pending CN117999482A (zh) | 2021-06-28 | 2022-06-27 | 用于测定信号扩增的方法、组合物和试剂盒 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230020070A1 (https=) |
| EP (1) | EP4363857A1 (https=) |
| JP (1) | JP2024526217A (https=) |
| KR (1) | KR20240051109A (https=) |
| CN (1) | CN117999482A (https=) |
| AU (1) | AU2022304570A1 (https=) |
| CA (1) | CA3225576A1 (https=) |
| IL (1) | IL309517A (https=) |
| TW (1) | TW202317992A (https=) |
| WO (1) | WO2023278293A1 (https=) |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4235601A (en) | 1979-01-12 | 1980-11-25 | Thyroid Diagnostics, Inc. | Test device and method for its use |
| US4442204A (en) | 1981-04-10 | 1984-04-10 | Miles Laboratories, Inc. | Homogeneous specific binding assay device and preformed complex method |
| US5310687A (en) | 1984-10-31 | 1994-05-10 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5591581A (en) | 1986-04-30 | 1997-01-07 | Igen, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| US6316607B1 (en) | 1986-04-30 | 2001-11-13 | Igen International, Inc. | Electrochemiluminescent assays |
| US5028535A (en) | 1989-01-10 | 1991-07-02 | Biosite Diagnostics, Inc. | Threshold ligand-receptor assay |
| US5786141A (en) | 1994-08-26 | 1998-07-28 | Bard; Allen J. | Electrogenerated chemiluminescence labels for analysis and/or referencing |
| US5679519A (en) * | 1995-05-09 | 1997-10-21 | Oprandy; John J. | Multi-label complex for enhanced sensitivity in electrochemiluminescence assay |
| US6136268A (en) | 1999-08-17 | 2000-10-24 | Orion Diagnostica | Method for luminescence measurements |
| US6291187B1 (en) * | 2000-05-12 | 2001-09-18 | Molecular Staging, Inc. | Poly-primed amplification of nucleic acid sequences |
| US6808939B2 (en) | 2001-06-29 | 2004-10-26 | Igen International, Inc. | ECL labels having improved non-specific binding properties, methods of using and kits containing the same |
| DK1412725T3 (en) | 2001-06-29 | 2019-03-25 | Meso Scale Technologies Llc | Multi-well plates for LUMINESCENSE TEST MEASUREMENTS |
| AU2002341621A1 (en) * | 2001-09-10 | 2003-03-24 | Meso Scale Technologies, Llc | Methods, reagents, kits and apparatus for protein function analysis |
| CA2772050C (en) | 2002-12-26 | 2016-09-06 | Meso Scale Technologies, Llc. | Assay cartridges and methods of using the same |
| US7981362B2 (en) | 2003-11-04 | 2011-07-19 | Meso Scale Technologies, Llc | Modular assay plates, reader systems and methods for test measurements |
| US7776583B2 (en) | 2004-06-03 | 2010-08-17 | Meso Scale Technologies, Llc | Methods and apparatuses for conducting assays |
| CA3179247A1 (en) | 2005-12-21 | 2007-07-05 | Meso Scale Technologies, Llc | Assay modules having assay reagents and methods of making and using same |
| WO2011017082A2 (en) | 2009-07-27 | 2011-02-10 | Clinton Charles M | Assay information management methods and devices |
| EP4060325B1 (en) | 2009-12-07 | 2024-08-21 | Meso Scale Technologies, LLC. | Assay cartridge reader |
| JP6343448B2 (ja) | 2010-07-27 | 2018-06-13 | メソ スケール テクノロジーズ エルエルシー | 消耗品データ管理 |
| CA2824404C (en) | 2011-01-06 | 2023-01-03 | Meso Scale Technologies, Llc | Assay cartridges for pcr analysis and methods of use thereof |
| JP6630566B2 (ja) | 2012-07-05 | 2020-01-15 | メソ スケール テクノロジーズ エルエルシー | 分析評価カートリッジのバルブシステム |
| JP2015526427A (ja) | 2012-08-02 | 2015-09-10 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｅｃｌ用の新規イリジウムベース錯体 |
| CA3235305A1 (en) | 2013-01-04 | 2014-07-10 | Meso Scale Technologies, Llc. | Assay apparatuses, methods and reagents |
| DK2972353T3 (da) | 2013-03-11 | 2023-03-27 | Meso Scale Technologies Llc | Forbedrede fremgangsmåder til udførelse af multipleksanalyser |
| US10114015B2 (en) * | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| CA2904181A1 (en) * | 2013-03-13 | 2014-10-09 | Anahit Aghvanyan | Sandwich immunoassay comprising anchoring reagent |
| EP3143401A4 (en) * | 2014-05-15 | 2017-10-11 | Meso Scale Technologies, LLC | Improved assay methods |
| US10725059B2 (en) | 2015-04-06 | 2020-07-28 | Meso Scale Technologies, Llc. | High throughput system for performing assays using electrochemiluminescence including a consumable shaking apparatus |
| US11300579B2 (en) | 2015-07-23 | 2022-04-12 | Meso Scale Technologies, Llc. | Integrated consumable data management system and platform |
| EP3488031B1 (en) | 2016-07-22 | 2024-07-31 | Meso Scale Technologies, LLC | Integrated consumable data management system & platform |
| AU2019418498A1 (en) | 2019-01-03 | 2021-07-15 | Meso Scale Technologies, Llc | Compositions and methods for carrying out assay measurements |
-
2022
- 2022-06-27 AU AU2022304570A patent/AU2022304570A1/en active Pending
- 2022-06-27 IL IL309517A patent/IL309517A/en unknown
- 2022-06-27 CN CN202280051025.7A patent/CN117999482A/zh active Pending
- 2022-06-27 WO PCT/US2022/035068 patent/WO2023278293A1/en not_active Ceased
- 2022-06-27 EP EP22747837.7A patent/EP4363857A1/en active Pending
- 2022-06-27 JP JP2023579630A patent/JP2024526217A/ja active Pending
- 2022-06-27 US US17/849,917 patent/US20230020070A1/en active Pending
- 2022-06-27 KR KR1020247002999A patent/KR20240051109A/ko active Pending
- 2022-06-27 CA CA3225576A patent/CA3225576A1/en active Pending
- 2022-06-28 TW TW111124084A patent/TW202317992A/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP4363857A1 (en) | 2024-05-08 |
| WO2023278293A1 (en) | 2023-01-05 |
| AU2022304570A1 (en) | 2024-01-18 |
| KR20240051109A (ko) | 2024-04-19 |
| CA3225576A1 (en) | 2023-01-05 |
| JP2024526217A (ja) | 2024-07-17 |
| IL309517A (en) | 2024-02-01 |
| US20230020070A1 (en) | 2023-01-19 |
| TW202317992A (zh) | 2023-05-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |