TW202317992A - 用於分析信號放大之方法、組合物及套組 - Google Patents

用於分析信號放大之方法、組合物及套組 Download PDF

Info

Publication number
TW202317992A
TW202317992A TW111124084A TW111124084A TW202317992A TW 202317992 A TW202317992 A TW 202317992A TW 111124084 A TW111124084 A TW 111124084A TW 111124084 A TW111124084 A TW 111124084A TW 202317992 A TW202317992 A TW 202317992A
Authority
TW
Taiwan
Prior art keywords
reagent
detectable label
detectable
binding
signal amplification
Prior art date
Application number
TW111124084A
Other languages
English (en)
Chinese (zh)
Inventor
約翰 肯頓
喬治 西格爾
施瓦茨 亞歷山大 Ｋ 塔克
Original Assignee
美商梅梭刻度技術公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 美商梅梭刻度技術公司 filed Critical 美商梅梭刻度技術公司
Publication of TW202317992A publication Critical patent/TW202317992A/zh

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
    • C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
    • C07F15/0046—Ruthenium compounds
    • C07F15/0053—Ruthenium compounds without a metal-carbon linkage
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
TW111124084A 2021-06-28 2022-06-28 用於分析信號放大之方法、組合物及套組 TW202317992A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163215660P 2021-06-28 2021-06-28
US63/215,660 2021-06-28

Publications (1)

Publication Number Publication Date
TW202317992A true TW202317992A (zh) 2023-05-01

Family

ID=82703171

Family Applications (1)

Application Number Title Priority Date Filing Date
TW111124084A TW202317992A (zh) 2021-06-28 2022-06-28 用於分析信號放大之方法、組合物及套組

Country Status (10)

Country Link
US (1) US20230020070A1 (https=)
EP (1) EP4363857A1 (https=)
JP (1) JP2024526217A (https=)
KR (1) KR20240051109A (https=)
CN (1) CN117999482A (https=)
AU (1) AU2022304570A1 (https=)
CA (1) CA3225576A1 (https=)
IL (1) IL309517A (https=)
TW (1) TW202317992A (https=)
WO (1) WO2023278293A1 (https=)

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4235601A (en) 1979-01-12 1980-11-25 Thyroid Diagnostics, Inc. Test device and method for its use
US4442204A (en) 1981-04-10 1984-04-10 Miles Laboratories, Inc. Homogeneous specific binding assay device and preformed complex method
US5310687A (en) 1984-10-31 1994-05-10 Igen, Inc. Luminescent metal chelate labels and means for detection
US5591581A (en) 1986-04-30 1997-01-07 Igen, Inc. Electrochemiluminescent rhenium moieties and methods for their use
US6316607B1 (en) 1986-04-30 2001-11-13 Igen International, Inc. Electrochemiluminescent assays
US5028535A (en) 1989-01-10 1991-07-02 Biosite Diagnostics, Inc. Threshold ligand-receptor assay
US5786141A (en) 1994-08-26 1998-07-28 Bard; Allen J. Electrogenerated chemiluminescence labels for analysis and/or referencing
US5679519A (en) * 1995-05-09 1997-10-21 Oprandy; John J. Multi-label complex for enhanced sensitivity in electrochemiluminescence assay
US6136268A (en) 1999-08-17 2000-10-24 Orion Diagnostica Method for luminescence measurements
US6291187B1 (en) * 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
US6808939B2 (en) 2001-06-29 2004-10-26 Igen International, Inc. ECL labels having improved non-specific binding properties, methods of using and kits containing the same
DK1412725T3 (en) 2001-06-29 2019-03-25 Meso Scale Technologies Llc Multi-well plates for LUMINESCENSE TEST MEASUREMENTS
AU2002341621A1 (en) * 2001-09-10 2003-03-24 Meso Scale Technologies, Llc Methods, reagents, kits and apparatus for protein function analysis
CA2772050C (en) 2002-12-26 2016-09-06 Meso Scale Technologies, Llc. Assay cartridges and methods of using the same
US7981362B2 (en) 2003-11-04 2011-07-19 Meso Scale Technologies, Llc Modular assay plates, reader systems and methods for test measurements
US7776583B2 (en) 2004-06-03 2010-08-17 Meso Scale Technologies, Llc Methods and apparatuses for conducting assays
CA3179247A1 (en) 2005-12-21 2007-07-05 Meso Scale Technologies, Llc Assay modules having assay reagents and methods of making and using same
WO2011017082A2 (en) 2009-07-27 2011-02-10 Clinton Charles M Assay information management methods and devices
EP4060325B1 (en) 2009-12-07 2024-08-21 Meso Scale Technologies, LLC. Assay cartridge reader
JP6343448B2 (ja) 2010-07-27 2018-06-13 メソ スケール テクノロジーズ エルエルシー 消耗品データ管理
CA2824404C (en) 2011-01-06 2023-01-03 Meso Scale Technologies, Llc Assay cartridges for pcr analysis and methods of use thereof
JP6630566B2 (ja) 2012-07-05 2020-01-15 メソ スケール テクノロジーズ エルエルシー 分析評価カートリッジのバルブシステム
JP2015526427A (ja) 2012-08-02 2015-09-10 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｅｃｌ用の新規イリジウムベース錯体
CA3235305A1 (en) 2013-01-04 2014-07-10 Meso Scale Technologies, Llc. Assay apparatuses, methods and reagents
DK2972353T3 (da) 2013-03-11 2023-03-27 Meso Scale Technologies Llc Forbedrede fremgangsmåder til udførelse af multipleksanalyser
US10114015B2 (en) * 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CA2904181A1 (en) * 2013-03-13 2014-10-09 Anahit Aghvanyan Sandwich immunoassay comprising anchoring reagent
EP3143401A4 (en) * 2014-05-15 2017-10-11 Meso Scale Technologies, LLC Improved assay methods
US10725059B2 (en) 2015-04-06 2020-07-28 Meso Scale Technologies, Llc. High throughput system for performing assays using electrochemiluminescence including a consumable shaking apparatus
US11300579B2 (en) 2015-07-23 2022-04-12 Meso Scale Technologies, Llc. Integrated consumable data management system and platform
EP3488031B1 (en) 2016-07-22 2024-07-31 Meso Scale Technologies, LLC Integrated consumable data management system & platform
AU2019418498A1 (en) 2019-01-03 2021-07-15 Meso Scale Technologies, Llc Compositions and methods for carrying out assay measurements

Also Published As

Publication number Publication date
EP4363857A1 (en) 2024-05-08
WO2023278293A1 (en) 2023-01-05
AU2022304570A1 (en) 2024-01-18
KR20240051109A (ko) 2024-04-19
CA3225576A1 (en) 2023-01-05
CN117999482A (zh) 2024-05-07
JP2024526217A (ja) 2024-07-17
IL309517A (en) 2024-02-01
US20230020070A1 (en) 2023-01-19

Similar Documents

Publication Publication Date Title
JP7769743B2 (ja) シグナル発生型デジタルアッセイにおけるノイズを低減する方法
US20220091108A1 (en) Dry-down processes for dye-conjugated reagents
US12181466B2 (en) Controls for implementing multiplex analysis methods
EP2639584A1 (en) Real time diagnostic assays using an evanescence biosensor
JP2017510811A (ja) スポットに分析方法を実行するための対照マーカー
US20190204310A1 (en) Multiplex measure of isotype antigen response
US20130165335A1 (en) Multiplex measure of isotype antigen response
JP4018746B1 (ja) 免疫学的測定法およびチップ
US20220155311A1 (en) Multiplexed Imaging with Nanobody Probes
TW202317992A (zh) 用於分析信號放大之方法、組合物及套組
US8969256B2 (en) Solution microarrays and uses thereof
JPH10511460A (ja) 被験サンプル中の複数分析物の時差式検出方法
CN105467113A (zh) 一种基于荧光素和萤光素酶生物发光反应的免疫分析方法
CA3152467A1 (en) Multiplex measure of isotype antigen response
RU2519023C2 (ru) Способ детектирования концентрации аналита с широким динамическим диапазоном
KR102734247B1 (ko) 면역어세이에서 간섭의 감소
US20140274758A1 (en) Antibody profiling, methods and apparatus for identifying an individual or source of a biological material
HK1234144B (zh) 用於执行多重分析方法的对照