CN1179211A - 室内灰尘的检测方法 - Google Patents

室内灰尘的检测方法 Download PDF

Info

Publication number
CN1179211A
CN1179211A CN96192747A CN96192747A CN1179211A CN 1179211 A CN1179211 A CN 1179211A CN 96192747 A CN96192747 A CN 96192747A CN 96192747 A CN96192747 A CN 96192747A CN 1179211 A CN1179211 A CN 1179211A
Authority
CN
China
Prior art keywords
aforementioned
dyestuff
house dust
detection agent
component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN96192747A
Other languages
English (en)
Other versions
CN1107872C (zh
Inventor
诺曼·辛克莱
拉尔夫·索尔
海克·波奇
沃尔夫冈·沃尔克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vorwerk and Co Interholding GmbH
Original Assignee
Vorwerk and Co Interholding GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19518287A external-priority patent/DE19518287A1/de
Application filed by Vorwerk and Co Interholding GmbH filed Critical Vorwerk and Co Interholding GmbH
Publication of CN1179211A publication Critical patent/CN1179211A/zh
Application granted granted Critical
Publication of CN1107872C publication Critical patent/CN1107872C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/683—Total protein determination, e.g. albumin in urine involving metal ions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

本发明涉及一种用于室内灰尘的检测方法,这些灰尘具有引起过敏性反应的潜在可能性,其特征是,在室内灰尘中掺入一种用于确定室内灰尘中所含的含蛋白质组分的数量级的检测剂;另外还涉及使用一种能够分辨蛋白质组分的检测组分以确定室内灰尘中引起过敏反应的潜在可能性的检测剂。

Description

室内灰尘的检测方法
本发明首先涉及一种室内灰尘的检测方法，这些灰尘具有引起过敏性反应的潜在可能性。
通常可以确定，在很大范围内越来越多地出现了过敏或变态性反应。特别是在住宅和家庭用品中存在的污染如螨排泄物、霉菌、花粉、草和桦树特定的植物部分以及皮肤鳞屑应对此负责。人们试图用已知的清洁技术来清除这些污染，然后将这些污染积聚作为室内灰尘组份而除掉。因此存在一种需求，即可以对室内灰尘的积聚和去除进行有效地检查。另外还存在一种需求，即确定室内灰尘中上述污染掺入的程度，作为过敏反应潜在可能性的尺度。还可以例如得到一种提示，寻找特殊污染来源。
由此可以看出，本发明要解决的问题是，提出一种检测室内灰尘的方法，以便可以推断室内灰尘引起过敏反应的潜在可能性。另一个要解决的技术问题是，提出一种对此适用的手段或设备。
所述的该问题首先基本上由权利要求1的主题所解决，其中为了检测室内灰尘而使用一种蛋白质检测来确定室内灰尘中含有的含蛋白质组份的数量级。本发明发现，实际上室内灰尘中的上述所有过敏组份都是由蛋白质构成的，或者说具有蛋白质组份。另外还发现，表征特别是这种室内灰尘中的含蛋白质组份的比例表征同时也是所涉及的室内灰尘引起过敏反应的潜在可能性的尺度。另一个要素是，蛋白质检测是一种优选与室内灰尘中的含蛋白质组份发生反应特别是发生变色反应沉淀的染料。该方法的这种方案可以简单的方式借助于颜色的强度确定室内灰尘中可能引起过敏反应的组份的数量级。为此要考虑按照蛋白质检测剂对室内灰尘的作用发生的颜色强度。在该方法的另一个优选的方案中，使用焦倍酚作为染料。具体为焦倍酚-红-Mo-络合物，其中另外在“焦倍酚”上加入。使用染料和特别是使用焦倍酚染料一般来检测室内灰尘也是本发明的另一个独立的主题。在焦倍酚中，蛋白质或含蛋白质的颗粒呈现紫色或蓝色色调的颜色，而染料本身起初是红色的。考虑到检测剂，建议该染料溶解在一种液体中。该液体表明可以作为一种成份特别含有一种缓冲剂和优选稳定剂。缓冲剂的作用是防止液体已经发生的PH值的变化引起染料变色或者颜色强度的变化。在另一个优选的方案中，考虑到应当用于检测最小量的室内灰尘，建议液体含有一种溶剂。优选的溶剂具体的是变性醇。例如50％的变性醇或者0.2mol HCL-甘油缓冲剂与96％的变性醇以1∶1的比例的混合物。该醇同时还具有有利的结合作用，使得有关的组份分解。偶尔存在的脂肪涂层或者脂肪组份可以溶解。
本发明的另一个主题是用于测定室内灰尘中引起过敏反应的可能性的检测剂。该检测剂的特征在于具有一种检测组份，它可以识别室内灰尘中蛋白质成份。该检测剂优选为一种染料，它优选与室内灰尘中的含蛋白质组份发生变色反应或沉淀，其中为了确定该组份的数量级，可以参考自我发生的颜色强度。该染料尤其可以是一种焦倍酚-红-Mo-络合物。该检测剂可以是一种染料溶解在其中的液体。该液体可以含有缓冲剂和优选稳定剂作为组份。另外，该液体还可以含有一种溶剂。也可以参考上述的解释。
上述检测液体或者检测剂的一个实施例的组成如下：
1.甘氨酸-HCl，作为缓冲组份，浓度为0.2mo/l，PH值为1.9，以1∶1的比例与96％的甲基乙基酮-变性醇相混合。
2.组份0.2g/l焦倍酚红
        -0.3g/l钼酸铵四水合物
        -1.0g/l草酸纳
        -1.2g/l(L+)-酒石酸
3.以1∶10的比例周0.2mol的与96％的变性醇以1∶1的比例相混合的HCl-甘氨酸稀释。
组份3应当是在液体作为室内灰尘变色试剂在悬浮液时使用。即使不添加组份3，该液体也可以作为浓缩的基本溶液用于例如吸尘器的过滤器的直接变色。
在使用时，例如将2-4滴浓缩的焦倍酚-红-钼-基本溶液滴到约1cm2大的加工成槽形的由聚丙烯组成的过滤件上。该过滤件应当浸透，但不要覆盖有过量的的溶液。根据环境温度和有关的细灰尘的数量和类型，过滤器中的灰尘物料最多5分钟即可根据蛋白质负荷而变色成蓝-紫色。
另一个使用的实例是以1∶10用50％的变性醇稀释的液体，(例如可对比上述实施例的组份3)。将1ml该焦倍酚-红-钼-络合物溶液与一种“刀尖(Messerspitze)”室内灰尘相混合。通过在一个热手中摇动一个含有液体的玻璃试管，加速向蓝-紫色的变色。在通常的室内灰尘中1-2分钟即可检出紫色。
这样表征的室内灰尘颗粒还可以进行显微镜分析。液体或染料对此后进行的分析的影响不大。这既适用于在过滤器中变色的颗粒，也适用于稀释变色的室内灰尘悬浮液。
作为唯一的附图是一个这里得到的显微镜照片的复印件。用X表示的颗粒彩色复印时显示出明显的蓝-紫色，而用Y表示的颗粒具有其它颜色或者基本上没有颜色。没有颜色的颗粒是例如沙粒或者糖粒。
在上述说明书、附图和权利要求书中公开的特征在既可以单独也可以随意组合来实现本发明时是重要的。所有公开的特征都是本发明的必要特征。在公开本发明时也可以完全包括所属/所附的优先权文件所公开的内容。

Claims (12)

1.一种室内灰尘的检测方法，这些灰尘具有引起过敏性反应的潜在可能性，其特征是，在室内灰尘中掺入一种用于确定室内灰尘中所含的含蛋白质组份的数量级的检测剂。
2.按照权利要求1所述的方法，其特征是，蛋白质检测剂是一种染料，它优选与含蛋白质组份沉淀发生变色反应，借助于自我发生的颜色强度来确定数量级。
3.按照前述权利要求中之一或多项所述的方法，其特征是，该染料是焦倍酚。
4.按照前述权利要求中之一或多项所述的方法，其特征是，染料溶解在一种液体中，它含有缓冲剂和优选稳定剂作为组份。
5.按照前述权利要求中之一或多项所述的方法，其特征是，液体含有一种溶剂。
6.按照前述权利要求中之一或多项所述的方法，其特征是，溶剂是约50％的变性醇。
7.用于确定室内灰尘引起过敏反应的潜在可能性的检测剂，含有一种能够分辨蛋白质组分的检测组份。
8.按照权利要求7所述的检测剂，其特征是，检测组份是一种染料。
9.按照前述权利要求7或8中之一所述的检测剂，其特征是，染料是焦倍酚。
10.按照前述权利要求7-9中之一所述的检测剂，其特征是，染料溶解在一种液体中，它含有一种缓冲剂和优选稳定剂作为组份。
11.按照前述权利要求7-10中之一或多项所述的检测剂，其特征是，液体含有一种溶剂。
12.按照前述权利要求7-11中之一或多项所述的检测剂，其特征是，溶剂是约50％的变性醇。
CN96192747A 1995-03-24 1996-03-15 确定室内灰尘引起过敏性反应的潜在可能性的方法 Expired - Lifetime CN1107872C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19510810.8 1995-03-24
DE19510810 1995-03-24
DE19518287.1 1995-05-18
DE19518287A DE19518287A1 (de) 1995-03-24 1995-05-18 Verfahren zur Untersuchung von Hausstaub

Publications (2)

Publication Number Publication Date
CN1179211A true CN1179211A (zh) 1998-04-15
CN1107872C CN1107872C (zh) 2003-05-07

Family

ID=26013692

Family Applications (1)

Application Number Title Priority Date Filing Date
CN96192747A Expired - Lifetime CN1107872C (zh) 1995-03-24 1996-03-15 确定室内灰尘引起过敏性反应的潜在可能性的方法

Country Status (12)

Country Link
US (1) US5981287A (zh)
EP (1) EP0815451B1 (zh)
JP (1) JPH11502621A (zh)
CN (1) CN1107872C (zh)
AT (1) ATE207617T1 (zh)
AU (1) AU5144696A (zh)
CZ (1) CZ292316B6 (zh)
DK (1) DK0815451T3 (zh)
ES (1) ES2163009T3 (zh)
PL (1) PL322449A1 (zh)
PT (1) PT815451E (zh)
WO (1) WO1996030764A1 (zh)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9913487D0 (en) * 1999-06-11 1999-08-11 Pirzad Ramin Dust mite detection
US8354647B2 (en) * 2000-11-08 2013-01-15 University Of North Florida Photoelectric chemical sensor and sensing method utilizing interfacial photo-voltages
US7892495B2 (en) * 2000-11-08 2011-02-22 Huebner Jay S Sensing device and method for rapidly determining concentrations of microbial organisms using interfacial photo-voltages
US7354770B2 (en) 2000-11-08 2008-04-08 University Of North Florida Sensing device and method using photo-induced charge movements
US20050238535A1 (en) * 2001-10-03 2005-10-27 20/20 Genesystems, Inc. Rapid assay, method and system for detecting biowarfare agents
AU2002367471A1 (en) * 2001-10-03 2003-11-03 20/20 Genesystems, Inc. Rapid assay, method and system for detecting biowarfare agents
US7033777B2 (en) * 2002-03-22 2006-04-25 Temple University Of The Commonwealth System Of Higher Education Method for detecting cockroach allergens and determining total allergen level
US20060182654A1 (en) * 2005-02-16 2006-08-17 Cumberland Brandi M Sampling device and method for the rapid detection of proteins in mold, allergens of other protein-containing substances
DE102005013623A1 (de) 2005-03-10 2006-09-14 Wolfgang Schallmey Verfahren zur Untersuchung und Bestimmung von proteinhaltigen Strukturen
GB2426334A (en) * 2005-05-20 2006-11-22 Orion Diagnostica Oy Application of a reagent to a matrix material
US7976639B2 (en) * 2007-08-17 2011-07-12 S.C. Johnson & Son, Inc. Method for determining the percentage of allergens picked up from a surface
US10260999B2 (en) * 2012-09-19 2019-04-16 Amichai Glattstein Sampling device
US10835897B2 (en) 2015-03-16 2020-11-17 Dots Technology Corp. Portable allergen detection system

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61155757A (ja) * 1984-12-27 1986-07-15 Wako Pure Chem Ind Ltd 微量蛋白定量法
JPS626170A (ja) * 1985-07-03 1987-01-13 Wako Pure Chem Ind Ltd 蛋白測定用試験片
US4960710A (en) * 1988-06-06 1990-10-02 Miles Inc. Device and method of assaying for trace mounts of proteins
US5087575A (en) * 1988-06-06 1992-02-11 Miles Inc. Composition for determining trace amount of protein
US5433948A (en) * 1990-02-13 1995-07-18 Thomas; Wayne R. Cloning and sequencing of allergens of dermatophagoides (house dust mite)
FR2687319B1 (fr) * 1992-02-14 1994-05-20 Michel Blanc Procede de decontamination et de detoxification applique au genie sanitaire de l'habitat.
NZ249579A (en) * 1992-03-05 1997-01-29 Edmund Bruce Mitchell Apparatus for collecting and detecting antigens such as microorganisms and allergenic disease causing agents from an environment such as a household environment

Also Published As

Publication number Publication date
JPH11502621A (ja) 1999-03-02
PL322449A1 (en) 1998-02-02
WO1996030764A1 (de) 1996-10-03
CZ292316B6 (cs) 2003-09-17
EP0815451A1 (de) 1998-01-07
CZ272797A3 (cs) 1998-02-18
EP0815451B1 (de) 2001-10-24
DK0815451T3 (da) 2002-03-04
CN1107872C (zh) 2003-05-07
ATE207617T1 (de) 2001-11-15
ES2163009T3 (es) 2002-01-16
PT815451E (pt) 2002-04-29
AU5144696A (en) 1996-10-16
US5981287A (en) 1999-11-09

Similar Documents

Publication Publication Date Title
CN1107872C (zh) 确定室内灰尘引起过敏性反应的潜在可能性的方法
Otero-Romaní et al. Evaluation of commercial C18 cartridges for trace elements solid phase extraction from seawater followed by inductively coupled plasma-optical emission spectrometry determination
Wei et al. Platinum analysis and speciation in urban gullypots
Wang et al. Dispersion-aggregation-dispersion colorimetric detection for mercury ions based on an assembly of gold nanoparticles and carbon nanodots
Jones Some observations on direct counts of freshwater bacteria obtained with a fluorescence microscope
Morquecho et al. Pollutant associations with particulates in stormwater
JPH09196923A (ja) タンパク質試薬としてのガラス／セルロース
CN115212729A (zh) 一种高稳定性水处理用超滤膜及其制备方法
US4912035A (en) Method for minimizing interference by reductants when detecting cells in biological fluids
Queiroz et al. Flow system with in-line separation/preconcentration coupled to graphite furnace atomic absorption spectrometry with W–Rh permanent modifier for copper determination in seawater
Yuan et al. Flow-injection column preconcentration directly coupled with electrothermal atomization atomic absorption spectrometry for the determination of aluminium. Comparison of column packing materials
JP4489590B2 (ja) 蛋白質測定用試験片およびその製造方法
Yoshimura et al. Automated high-performance liquid chromatographic determination of hydroxylysylpyridinoline and lysylpyridinoline in urine using a column-switching method
JPH09203735A (ja) 非特異反応の抑制方法
JP3204030B2 (ja) セレン汚染土壌の不溶化処理方法
JP3538912B2 (ja) 飛灰の性状測定方法及び薬剤処理方法
JP2000301162A (ja) フロアーワックス剥離廃液の処理剤およびその処理方法
CN109126290A (zh) 一种高强度阻燃空气过滤材料及其制备方法
JP2004257920A (ja) 鋼中析出物中炭素の定量分析方法
Hsieh et al. Robotic sample preparation and high-performance liquid chromatographic analysis of verlukast in human plasma
US4474885A (en) Method of analyzing fuel oil for catalyst component
KR19980703142A (ko) 집 먼지의 조사 방법
Robinson The automated analysis of catecholamines: An improved procedure for the simultaneous differential analysis of epinephrine and norepinephrine in tissues, blood and gland perfusates
JP3479655B2 (ja) 高速液体クロマトグラフィによるビリルビンの測定方法
Baysal et al. Slurry analysis after lead collection on a sorbent and its determination by electrothermal atomic absorption spectrometry

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1036656

Country of ref document: HK

CX01 Expiry of patent term

Granted publication date: 20030507

EXPY Termination of patent right or utility model