CN117427017B - 一种含牡丹提取物的润肤抗皱面霜及其制备方法 - Google Patents
一种含牡丹提取物的润肤抗皱面霜及其制备方法 Download PDFInfo
- Publication number
- CN117427017B CN117427017B CN202311767655.7A CN202311767655A CN117427017B CN 117427017 B CN117427017 B CN 117427017B CN 202311767655 A CN202311767655 A CN 202311767655A CN 117427017 B CN117427017 B CN 117427017B
- Authority
- CN
- China
- Prior art keywords
- parts
- polysaccharide
- carrying
- peony
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000736199 Paeonia Species 0.000 title claims abstract description 49
- 235000006484 Paeonia officinalis Nutrition 0.000 title claims abstract description 49
- 239000006071 cream Substances 0.000 title claims abstract description 33
- 230000001153 anti-wrinkle effect Effects 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 230000001815 facial effect Effects 0.000 title claims abstract description 13
- 230000003020 moisturizing effect Effects 0.000 title claims description 15
- 238000000605 extraction Methods 0.000 title description 3
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 67
- 239000005017 polysaccharide Substances 0.000 claims abstract description 67
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 62
- 239000000843 powder Substances 0.000 claims abstract description 58
- 150000004676 glycans Chemical class 0.000 claims abstract description 51
- 241000206572 Rhodophyta Species 0.000 claims abstract description 35
- 150000001875 compounds Chemical class 0.000 claims abstract description 35
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 28
- 108060006184 phycobiliprotein Proteins 0.000 claims abstract description 28
- 230000004048 modification Effects 0.000 claims abstract description 17
- 238000012986 modification Methods 0.000 claims abstract description 17
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 11
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 claims abstract description 11
- SNPLKNRPJHDVJA-ZETCQYMHSA-N D-panthenol Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCCO SNPLKNRPJHDVJA-ZETCQYMHSA-N 0.000 claims abstract description 11
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 claims abstract description 11
- ZAKOWWREFLAJOT-UHFFFAOYSA-N d-alpha-Tocopheryl acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-UHFFFAOYSA-N 0.000 claims abstract description 11
- FOYKKGHVWRFIBD-UHFFFAOYSA-N gamma-tocopherol acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 FOYKKGHVWRFIBD-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000011187 glycerol Nutrition 0.000 claims abstract description 11
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 11
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 11
- XUGNVMKQXJXZCD-UHFFFAOYSA-N isopropyl palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC(C)C XUGNVMKQXJXZCD-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229940101267 panthenol Drugs 0.000 claims abstract description 11
- 235000020957 pantothenol Nutrition 0.000 claims abstract description 11
- 239000011619 pantothenol Substances 0.000 claims abstract description 11
- 238000013329 compounding Methods 0.000 claims abstract description 7
- 239000002131 composite material Substances 0.000 claims description 27
- 238000002156 mixing Methods 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 20
- 239000008367 deionised water Substances 0.000 claims description 19
- 229910021641 deionized water Inorganic materials 0.000 claims description 19
- 238000004108 freeze drying Methods 0.000 claims description 19
- -1 compound polysaccharide Chemical class 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 8
- 229960001471 sodium selenite Drugs 0.000 claims description 8
- 239000011781 sodium selenite Substances 0.000 claims description 8
- 235000015921 sodium selenite Nutrition 0.000 claims description 8
- 108010059892 Cellulase Proteins 0.000 claims description 7
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 7
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 7
- 229940106157 cellulase Drugs 0.000 claims description 7
- 229910017604 nitric acid Inorganic materials 0.000 claims description 7
- 238000000967 suction filtration Methods 0.000 claims description 7
- WSGCRSMLXFHGRM-DEVHWETNSA-N (2s)-2-[[(2s)-6-amino-2-[[(2s,3r)-2-[[(2s,3r)-2-[[(2s)-6-amino-2-(hexadecanoylamino)hexanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxybutanoyl]amino]hexanoyl]amino]-3-hydroxypropanoic acid Chemical compound CCCCCCCCCCCCCCCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O WSGCRSMLXFHGRM-DEVHWETNSA-N 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 230000008961 swelling Effects 0.000 claims description 6
- 241000195474 Sargassum Species 0.000 claims description 5
- 229960005070 ascorbic acid Drugs 0.000 claims description 5
- 235000010323 ascorbic acid Nutrition 0.000 claims description 5
- 239000011668 ascorbic acid Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 229960005150 glycerol Drugs 0.000 claims description 5
- 229940075495 isopropyl palmitate Drugs 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 238000009210 therapy by ultrasound Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 13
- 125000001312 palmitoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 abstract description 4
- 239000000654 additive Substances 0.000 abstract description 3
- 239000005556 hormone Substances 0.000 abstract description 3
- 229940088597 hormone Drugs 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 31
- 238000012360 testing method Methods 0.000 description 15
- 150000003254 radicals Chemical class 0.000 description 10
- 230000002292 Radical scavenging effect Effects 0.000 description 9
- 239000000047 product Substances 0.000 description 8
- 241000251468 Actinopterygii Species 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 230000037303 wrinkles Effects 0.000 description 6
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 241000195493 Cryptophyta Species 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- OCZVHBZNPVABKX-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine;ethanol Chemical compound CCO.[O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 OCZVHBZNPVABKX-UHFFFAOYSA-N 0.000 description 2
- 241001474374 Blennius Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 125000003172 aldehyde group Chemical group 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- MCAHWIHFGHIESP-UHFFFAOYSA-L selenite(2-) Chemical group [O-][Se]([O-])=O MCAHWIHFGHIESP-UHFFFAOYSA-L 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
- ZTOJFFHGPLIVKC-CLFAGFIQSA-N abts Chemical compound S/1C2=CC(S(O)(=O)=O)=CC=C2N(CC)C\1=N\N=C1/SC2=CC(S(O)(=O)=O)=CC=C2N1CC ZTOJFFHGPLIVKC-CLFAGFIQSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 229960003082 galactose Drugs 0.000 description 1
- 108010025899 gelatin film Proteins 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000008832 photodamage Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229940091258 selenium supplement Drugs 0.000 description 1
- 230000036548 skin texture Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
- A61K8/675—Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9711—Phaeophycota or Phaeophyta [brown algae], e.g. Fucus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9717—Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Emergency Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
本发明公开了面霜技术领域的一种含牡丹提取物的润肤抗皱面霜及其制备方法,包括如下重量份的组分:多元改性复配凝胶30‑40份、甘油10‑12份、透明质酸3‑5份、棕榈酸异丙酯1.8‑2.2份、烟酰胺0.6‑0.8份、泛酞醇0.15‑0.3份、棕榈酰五肽‑40.06‑0.08份、生育酚乙酸酯0.8‑1份、水100‑120份;多元改性复配凝胶是以牡丹花蕾‑马尾藻粉末提取多糖并进行硒化改性后与红藻粉末提取的藻胆蛋白复配制备的。本发明提出通过提取牡丹多糖、马尾藻多糖、红藻藻胆蛋白作为有效抗皱成分的方式,实现了避免不良添加剂和激素的使用,多糖硒化改性与藻胆蛋白制备凝胶,进而实现见效快保质期长的技术效果。
Description
技术领域
本发明属于面霜技术领域,具体是指一种含牡丹提取物的润肤抗皱面霜及其制备方法。
背景技术
面霜是一种用于面部护理的化妆品,通常用于滋润、保湿和滋养皮肤,通常含有各种保湿成分、抗氧化剂、维生素和其他活性成分,以帮助保持皮肤的水分和健康状态,主要功能包括保湿、滋润滋养、修护、防护等;高分子多糖按其来源可分为植物多糖、动物多糖、微生物多糖和海藻多糖,其中植物多糖品种多、产品功能性复杂,马尾藻是一种常见的海藻,富含多种营养成分,其中包括多糖类物质,马尾藻多糖具有保湿滋润、抗氧化、抗炎、修复皮肤等功效;藻胆蛋白是一种来源于藻类的蛋白质,在藻类中起着类似于植物叶绿素的作用,可以帮助藻类进行光合作用,藻胆蛋白具有抗氧化、保护皮肤、保湿滋润、修复皮肤等多种作用,常常被用于面霜、乳液、面膜等化妆品或护肤品中,牡丹多糖是从牡丹花中提取得到的多糖类物质,具有抗氧化、保湿滋润、促进皮肤的修复和再生的作用,有助于改善皮肤质地和肤色。
目前现有技术主要存在以下问题:1.目前抗皱产品中的添加剂以及激素物质有安全隐患;2.天然植物来源的成分,存在见效慢,保质期短的问题。
发明内容
针对上述情况,为克服现有技术的缺陷,本发明提供了一种含牡丹提取物的润肤抗皱面霜及其制备方法,为了解决抗皱产品的安全问题问题,本发明提出通过提取牡丹多糖、马尾藻多糖、红藻藻胆蛋白作为有效抗皱成分的方式,实现了避免不良添加剂和激素的使用,多糖硒化改性,增强抗氧化能力,并与藻胆蛋白制备凝胶,进而实现见效快保质期长的技术效果。
为了实现上述目的,本发明采取的技术方案如下:本发明提出了一种含牡丹提取物的润肤抗皱面霜及其制备方法,所述含牡丹提取物的润肤抗皱面霜包括如下重量份的组分:多元改性复配凝胶30-40份、甘油10-12份、透明质酸3-5份、棕榈酸异丙酯1.8-2.2份、烟酰胺0.6-0.8份、泛酞醇0.15-0.3份、棕榈酰五肽-40.06-0.08份、生育酚乙酸酯0.8-1份、水100-120份;所述多元改性复配凝胶是以牡丹花蕾-马尾藻粉末提取多糖并进行硒化改性后与红藻粉末提取的藻胆蛋白复配制备的。
优选地,所述多元改性复配凝胶包括如下重量份的组分:牡丹花蕾35-40份、红藻粉末8-14份、马尾藻粉末23-28份、水10-15份。
优选地,所述多元改性复配凝胶的制备方法,具体包括如下步骤:
S1、选取新鲜的牡丹花蕾清洗,冻干粉碎,过120目筛,加入马尾藻粉末混合均匀,得到复合粉末;
S2、将S1所得复合粉末加入去离子水中,调节pH为4-5,加入纤维素酶,加入果胶酶,酶解2-3h,真空抽滤得到酶解液,加入酶解液3倍体积的无水乙醇溶液,4℃条件下冷藏10h,以3000-3500rpm离心5-10min,过滤,无水乙醇洗涤3次,冷冻干燥得到牡丹-马尾藻多糖;
S3、将S2所得牡丹-马尾藻多糖加入到质量浓度为0.035-0.045%的硝酸溶液中,混合均匀得到混合液,将亚硒酸钠加入到混合液中,70-80℃水浴60-80min,冷却至室温调节pH为5-6,4000rpm离心5min,抽滤后以抗坏血酸为显色剂,去离子水透析至无红色,进行减压浓缩,冷冻干燥得到硒化复合多糖;
S4、将红藻粉末加入到去离子水中,浸泡4h,充分溶胀,冰浴条件下进行60-80w超声处理5s停止15s,进行10次循环,在4℃下6500-7000rpm离心10min,取上清液,将硫酸铵以1-1.2mg/mL的添加量加入上清液中,30-60rpm搅拌1h,降温至4℃以9000-10000rpm离心8-10min,过滤冷冻干燥得到红藻藻胆蛋白;
S5、将S3所得硒化复合多糖与S4所得红藻藻胆蛋白混合均匀,得到多糖-蛋白混合物,将多糖-蛋白混合物加入水中,100-120rpm速度70-85℃水浴搅拌5-6min,得到多元改性复配凝胶。
优选地,在S2中,所述复合粉末在去离子水中的添加量为0.12-0.15g/mL;
优选地,在S2中,所述纤维素酶的添加量为复合粉末质量分数的2-3%;
优选地,在S2中,所述果胶酶的添加量为复合粉末质量分数的1-2%;
优选地,在S3中,所述牡丹-马尾藻多糖在硝酸溶液中的添加量为8-12mg/mL;
优选地,在S3中,所述亚硒酸钠在混合液中的添加量为10-15mg/mL;
优选地,在S4中,所述红藻粉末在去离子水中的添加量为60-80mg/mL;
优选地,在S4中,所述硫酸铵在上清液中的添加量为1-1.2mg/mL。
本发明还提供了一种含牡丹提取物的润肤抗皱面霜的制备方法,具体包括如下步骤:将甘油、透明质酸、棕榈酸异丙酯、烟酰胺、泛酞醇、棕榈酰五肽-4、生育酚乙酸酯、加入水中,搅拌均匀,然后加入多元改性复配凝胶,冰浴条件下,1000-1200rpm搅拌5-8min,混合均匀得到含牡丹提取物的润肤抗皱面霜。
本发明取得的有益效果如下:本发明通过对牡丹花蕾和马尾藻粉末进行酶解提取,通过醇沉离心的方式得到具有高活性的牡丹-马尾藻多糖,使用亚硒酸钠对复合多糖进行改性,纯化后得到硒化复合多糖,使用溶胀和超声的技术手段提取红藻粉末中的藻胆蛋白,将硒化后的复合多糖与红藻藻胆蛋白交联制备得到多元改性复配凝胶;牡丹多糖由β-构型半乳吡喃糖为主的酸性多糖组成具有极高的抗氧化活性,并能通过调节超氧化物歧化酶的活性、肿瘤坏死因子-α等细胞因子的水平达到抗光损伤的效果,马尾藻多糖富含硫酸盐多糖具有如抗氧化、抗菌等多种药理活性,硒化改性强化了牡丹-马尾藻多糖吸附重金属离子的能力并提高其抗氧化活性,亚硒酸酯基团活泼的活泼性质有利于与自由基的氮原子中心孤对电子配对,硒化修饰可以增加复合多糖对自由基的清除效果,过氧化物酶在硒的作用下,催化过氧化氢的还原和降解,可清除人体内的其他自由基,同时对减少皮肤水分和胶原蛋白的流失具有协同增效的作用;红藻藻胆蛋白具有良好的抗氧化性和抗炎活性,复合多糖与红藻藻胆蛋白的复配共混,以氨基醛基等基团相互反应交联,且硒化改性大大提高了两者结合的稳定性以及结合后蛋白二级、三级结构稳定性,增强凝胶成膜性能保水能力和吸收效果。
附图说明
图1为本发明实施例1-3、对比例1-2DPPH自由基清除能力测试的结果图;
图2为本发明实施例1-3、对比例1-2ABTS自由基清除能力测试的结果图;
图3为本发明实施例1-3、对比例1-2和对照组皮肤含水量测试的结果图;
图4为本发明实施例1-3、对比例1-2和对照组皮肤鱼尾纹面积测试的结果图。
附图用来提供对本发明的进一步理解,并且构成说明书的一部分,与本发明的实施例一起用于解释本发明,并不构成对本发明的限制。
具体实施方式
下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例;基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
除非另行定义,文中所使用的所有专业与科学用语与本领域技术人员所熟悉的意义相同。此外,任何与所记载内容相似或均等的方法及材料皆可应用于本发明中。文中所述的较佳实施方法与材料仅作示范之用,但不能限制本申请的内容。
下述实施例中的实验方法,如无特殊说明,均为常规方法;下述实施例中所用的试验材料及试验菌株,如无特殊说明,均为从商业渠道购买得到的。
实施例1
一种含牡丹提取物的润肤抗皱面霜及其制备方法,所述含牡丹提取物的润肤抗皱面霜包括如下重量份的组分:多元改性复配凝胶40份、甘油12份、透明质酸5份、棕榈酸异丙酯2.2份、烟酰胺0.8份、泛酞醇0.3份、棕榈酰五肽-40.08份、生育酚乙酸酯1份、水120份;所述多元改性复配凝胶是以牡丹花蕾-马尾藻粉末提取多糖并进行硒化改性后与红藻粉末提取的藻胆蛋白复配制备的。
优选地,所述多元改性复配凝胶包括如下重量份的组分:牡丹花蕾40份、红藻粉末14份、马尾藻粉末28份、水15份。
优选地,所述多元改性复配凝胶的制备方法,具体包括如下步骤:
S1、选取新鲜的牡丹花蕾清洗,冻干粉碎,过120目筛,加入马尾藻粉末混合均匀,得到复合粉末;
S2、将S1所得复合粉末以0.15g/mL的添加量加入去离子水中,调节pH为5,加入复合粉末质量分数3%的纤维素酶,加入复合粉末质量分数2%的果胶酶,酶解3h,真空抽滤得到酶解液,加入酶解液3倍体积的无水乙醇溶液,4℃条件下冷藏10h,以3500rpm离心10min,过滤,无水乙醇洗涤3次,冷冻干燥得到牡丹-马尾藻多糖;
S3、将S2所得牡丹-马尾藻多糖以12mg/mL的添加量加入到质量浓度为0.045%的硝酸溶液中,混合均匀得到混合液,将亚硒酸钠以15mg/mL的添加量加入到混合液中,80℃水浴80min,冷却至室温调节pH为6,4000rpm离心5min,抽滤后以抗坏血酸为显色剂,去离子水透析至无红色,进行减压浓缩,冷冻干燥得到硒化复合多糖;
S4、将红藻粉末以80mg/mL的添加量加入到去离子水中,浸泡4h,充分溶胀,冰浴条件下进行80w超声处理5s停止15s,进行10次循环,在4℃下7000rpm离心10min,取上清液,将硫酸铵以1.2mg/mL的添加量加入上清液中,60rpm搅拌1h,降温至4℃以10000rpm离心10min,过滤冷冻干燥得到红藻藻胆蛋白;
S5、将S3所得硒化复合多糖与S4所得红藻藻胆蛋白混合均匀,得到多糖-蛋白混合物,将多糖-蛋白混合物加入水中,120rpm速度85℃水浴搅拌6min,得到多元改性复配凝胶。
本发明还提供了一种含牡丹提取物的润肤抗皱面霜的制备方法,具体包括如下步骤:将甘油、透明质酸、棕榈酸异丙酯、烟酰胺、泛酞醇、棕榈酰五肽-4、生育酚乙酸酯、加入水中,搅拌均匀,然后加入多元改性复配凝胶,冰浴条件下,1200rpm搅拌8min,混合均匀得到含牡丹提取物的润肤抗皱面霜。
实施例2
一种含牡丹提取物的润肤抗皱面霜及其制备方法,所述含牡丹提取物的润肤抗皱面霜包括如下重量份的组分:多元改性复配凝胶30份、甘油10份、透明质酸3份、棕榈酸异丙酯1.8份、烟酰胺0.6份、泛酞醇0.15份、棕榈酰五肽-40.06份、生育酚乙酸酯0.8份、水100份;所述多元改性复配凝胶是以牡丹花蕾-马尾藻粉末提取多糖并进行硒化改性后与红藻粉末提取的藻胆蛋白复配制备的。
优选地,所述多元改性复配凝胶包括如下重量份的组分:牡丹花蕾35份、红藻粉末8份、马尾藻粉末23份、水10份。
优选地,所述多元改性复配凝胶的制备方法,具体包括如下步骤:
S1、选取新鲜的牡丹花蕾清洗,冻干粉碎,过120目筛,加入马尾藻粉末混合均匀,得到复合粉末;
S2、将S1所得复合粉末以0.12g/mL的添加量加入去离子水中,调节pH为4,加入复合粉末质量分数2%的纤维素酶,加入复合粉末质量分数1%的果胶酶,酶解2h,真空抽滤得到酶解液,加入酶解液3倍体积的无水乙醇溶液,4℃条件下冷藏10h,以3000rpm离心5min,过滤,无水乙醇洗涤3次,冷冻干燥得到牡丹-马尾藻多糖;
S3、将S2所得牡丹-马尾藻多糖以8mg/mL的添加量加入到质量浓度为0.035%的硝酸溶液中,混合均匀得到混合液,将亚硒酸钠以10mg/mL的添加量加入到混合液中,70℃水浴60min,冷却至室温调节pH为5,4000rpm离心5min,抽滤后以抗坏血酸为显色剂,去离子水透析至无红色,进行减压浓缩,冷冻干燥得到硒化复合多糖;
S4、将红藻粉末以60mg/mL的添加量加入到去离子水中,浸泡4h,充分溶胀,冰浴条件下进行60w超声处理5s停止15s,进行10次循环,在4℃下6500rpm离心10min,取上清液,将硫酸铵以1mg/mL的添加量加入上清液中,30rpm搅拌1h,降温至4℃以9000rpm离心8min,过滤冷冻干燥得到红藻藻胆蛋白;
S5、将S3所得硒化复合多糖与S4所得红藻藻胆蛋白混合均匀,得到多糖-蛋白混合物,将多糖-蛋白混合物加入水中,100rpm速度70℃水浴搅拌5min,得到多元改性复配凝胶。
本发明还提供了一种含牡丹提取物的润肤抗皱面霜的制备方法,具体包括如下步骤:将甘油、透明质酸、棕榈酸异丙酯、烟酰胺、泛酞醇、棕榈酰五肽-4、生育酚乙酸酯、加入水中,搅拌均匀,然后加入多元改性复配凝胶,冰浴条件下,1000rpm搅拌5min,混合均匀得到含牡丹提取物的润肤抗皱面霜。
实施例3
一种含牡丹提取物的润肤抗皱面霜及其制备方法,所述含牡丹提取物的润肤抗皱面霜包括如下重量份的组分:多元改性复配凝胶35份、甘油11份、透明质酸4份、棕榈酸异丙酯2份、烟酰胺0.7份、泛酞醇0.2份、棕榈酰五肽-40.07份、生育酚乙酸酯0.9份、水110份;所述多元改性复配凝胶是以牡丹花蕾-马尾藻粉末提取多糖并进行硒化改性后与红藻粉末提取的藻胆蛋白复配制备的。
优选地,所述多元改性复配凝胶包括如下重量份的组分:牡丹花蕾38份、红藻粉末10份、马尾藻粉末25份、水13份。
优选地,所述多元改性复配凝胶的制备方法,具体包括如下步骤:
S1、选取新鲜的牡丹花蕾清洗,冻干粉碎,过120目筛,加入马尾藻粉末混合均匀,得到复合粉末;
S2、将S1所得复合粉末以0.13g/mL的添加量加入去离子水中,调节pH为4.5,加入复合粉末质量分数2.5%的纤维素酶,加入复合粉末质量分数1.5%的果胶酶,酶解2.5h,真空抽滤得到酶解液,加入酶解液3倍体积的无水乙醇溶液,4℃条件下冷藏10h,以3200rpm离心7min,过滤,无水乙醇洗涤3次,冷冻干燥得到牡丹-马尾藻多糖;
S3、将S2所得牡丹-马尾藻多糖以10mg/mL的添加量加入到质量浓度为0.04%的硝酸溶液中,混合均匀得到混合液,将亚硒酸钠以13mg/mL的添加量加入到混合液中,75℃水浴70min,冷却至室温调节pH为5.5,4000rpm离心5min,抽滤后以抗坏血酸为显色剂,去离子水透析至无红色,进行减压浓缩,冷冻干燥得到硒化复合多糖;
S4、将红藻粉末以70mg/mL的添加量加入到去离子水中,浸泡4h,充分溶胀,冰浴条件下进行70w超声处理5s停止15s,进行10次循环,在4℃下6800rpm离心10min,取上清液,将硫酸铵以1.1mg/mL的添加量加入上清液中,50rpm搅拌1h,降温至4℃以9500rpm离心9min,过滤冷冻干燥得到红藻藻胆蛋白;
S5、将S3所得硒化复合多糖与S4所得红藻藻胆蛋白混合均匀,得到多糖-蛋白混合物,将多糖-蛋白混合物加入水中,110rpm速度80℃水浴搅拌5.5min,得到多元改性复配凝胶。
本发明还提供了一种含牡丹提取物的润肤抗皱面霜的制备方法,具体包括如下步骤:将甘油、透明质酸、棕榈酸异丙酯、烟酰胺、泛酞醇、棕榈酰五肽-4、生育酚乙酸酯、加入水中,搅拌均匀,然后加入多元改性复配凝胶,冰浴条件下,1100rpm搅拌6min,混合均匀得到含牡丹提取物的润肤抗皱面霜。
对比例1
本对比例提供一种面霜,其与实施例1的区别仅在于所有组分中牡丹-马尾藻多糖不进行改性,其余组分、组分含量与实施例1相同。
对比例2
本对比例提供一种面霜,其与实施例1的区别仅在于所有组分中硒化复合多糖不与蛋白反应复配,即无步骤S5中的水浴加热,其余组分、组分含量与实施例1相同。
实验例
1.DPPH自由基清除能力测试
实验组取实施例1-3、对比例1-2样品分别加入2mL的0.01%DPPH-乙醇溶液中,对照组取实施例1-3、对比例1-2样品分别加入2mL的无水乙醇中,空白组为2mL的0.01%DPPH-乙醇溶液中,摇匀在暗处反应30min,用紫外分光光度计测定517nm处的吸光值,以如下方法计算DPPH自由基清除率:
DPPH自由基清除率=[1-(实验组吸光值-对照组吸光值)/空白组吸光值]×100%。
图1为本发明实施例1-3、对比例1-2DPPH自由基清除能力测试的结果图;如图,实施例1-3、对比例1-2DPPH自由基清除率分别为83.2%、82.9%、85.1%、56.3%、78.5%;实施例1-3DPPH自由基清除率明显大于对比例1,说明牡丹-马尾藻多糖的改性提高了产品对DPPH自由基清除效果,实施例1-3DPPH自由基清除率明显大于对比例2,说明硒化复合多糖与蛋白反应复配提高了产品对DPPH自由基清除效果;亚硒酸酯基团活泼的活泼性质有利于与自由基的氮原子中心孤对电子配对,硒化修饰可以增加复合多糖对自由基的清除效果,复合多糖与红藻藻胆蛋白的复配共混,以氨基醛基等基团相互反应交联,且硒化改性大大提高了两者结合的稳定性保水能力和吸收效果得到提升。
2.ABTS自由基清除能力测试
将7mmol/LABTS母液与2.45mmol/L过硫酸钾混合,在室温下暗反应16h,用10mmol/L磷酸盐缓冲液(PH6.6)稀释,直至在734nm处的吸光度为0.70±0.02Abs,得到ABTS工作液,实验组取实施例1-3、对比例1-2样品分别加入2mLABTS工作液中,对照组取实施例1-3、对比例1-2样品分别加入2mL的水中,空白组为2mL的ABTS工作液,室温暗反应反应6min,用紫外分光光度仪在734nm处测定吸光度。ABTS自由基清除率计算方法如下:
ABTS自由基清除率=[1-(实验组吸光值-对照组吸光值)/空白组吸光值]×100%。
图2为本发明实施例1-3、对比例1-2ABTS自由基清除能力测试的结果图;如图,实施例1-3、对比例1-2ABTS自由基清除率分别为85.6%、83.8%、84.3%、59.4%、75.7%;实施例1-3ABTS自由基清除率明显大于对比例1,说明牡丹-马尾藻多糖的改性提高了产品对ABTS自由基清除效果,实施例1-3ABTS自由基清除率明显大于对比例2,说明硒化复合多糖与蛋白反应复配提高了产品对ABTS自由基清除效果。
3.皮肤含水量测试
在年龄40-45岁的女性志愿者中每组随机抽取10人,实验组分别使用实施例1-3和对比例1-2,对照组使用生理盐水,早晚各使用一次,六周后,使用皮肤水分测量仪对受试者测试部位进行皮肤含水量测定,取其平均值为皮肤含水量。
图3为本发明实施例1-3、对比例1-2和对照组皮肤含水量测试的结果图;如图,实施例1-3、对比例1-2和对照组皮肤含水量分别为82.8%、81.6%、82.3%、76.3%、78.1%、72.6%;实施例1-3皮肤含水量明显大于对比例1,且都大于对照组,说明牡丹-马尾藻多糖的改性提高了使用后皮肤含水量,实施例1-3皮肤含水量明显大于对比例2,且都大于对照组,说明硒化复合多糖与蛋白反应复配提高了使用后皮肤含水量。
4.皮肤鱼尾纹面积测试
使用面部图像分析仪采集面部皮肤图像并对鱼尾纹面积进行分析,以如下公式计算除皱率:除皱率=(0周鱼尾纹面积-6周鱼尾纹面积)/0周鱼尾纹面积×100%。
图4为本发明实施例1-3、对比例1-2和对照组皮肤鱼尾纹面积测试的结果图;如图,实施例1-3、对比例1-2除皱率分别为22.9%、24.1%、23.6%、10.2%、13.1%;实施例1-3除皱率明显大于对比例1,说明牡丹-马尾藻多糖的改性提高了除皱率,实施例1-3除皱率明显大于对比例2,且都大于对照组,说明硒化复合多糖与蛋白反应复配提高了除皱率。
尽管已经示出和描述了本发明的实施例,对于本领域的普通技术人员而言,可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型,本发明的范围由所附权利要求及其等同物限定。
以上对本发明及其实施方式进行了描述,这种描述没有限制性,附图中所示的也只是本发明的实施方式之一,实际的应用并不局限于此。总而言之如果本领域的普通技术人员受其启示,在不脱离本发明创造宗旨的情况下,不经创造性的设计出与该技术方案相似的方式及实施例,均应属于本发明的保护范围。
Claims (8)
1. 一种含牡丹提取物的润肤抗皱面霜,其特征在于:包括如下重量份的组分:多元改性复配凝胶30-40份、甘油10-12份、透明质酸3-5份、棕榈酸异丙酯1.8-2.2份、烟酰胺0.6-0.8份、泛酞醇0.15-0.3份、棕榈酰五肽-4 0.06-0.08份、生育酚乙酸酯0.8-1份和水100-120份;所述多元改性复配凝胶是以牡丹花蕾-马尾藻粉末提取多糖并进行硒化改性后与红藻粉末提取的藻胆蛋白复配制备的;
所述多元改性复配凝胶包括如下重量份的组分:牡丹花蕾35-40份、红藻粉末8-14份、马尾藻粉末23-28份和水10-15份;
所述多元改性复配凝胶的制备方法,具体包括如下步骤:
S1、选取新鲜的牡丹花蕾清洗,冻干粉碎,过120目筛,加入马尾藻粉末混合均匀,得到复合粉末;
S2、将S1所得复合粉末加入去离子水中,调节pH为4-5,加入纤维素酶,加入果胶酶,酶解2-3h,真空抽滤得到酶解液,加入酶解液3倍体积的无水乙醇溶液,4℃条件下冷藏10h,以3000-3500rpm离心5-10min,过滤,无水乙醇洗涤3次,冷冻干燥得到牡丹-马尾藻多糖;
S3、将S2所得牡丹-马尾藻多糖加入到质量浓度为0.035-0.045%的硝酸溶液中,混合均匀得到混合液,将亚硒酸钠加入到混合液中,70-80℃水浴60-80min,冷却至室温调节pH为5-6,4000rpm离心5min,抽滤后以抗坏血酸为显色剂,去离子水透析至无红色,进行减压浓缩,冷冻干燥得到硒化复合多糖;
S4、将红藻粉末加入到去离子水中,浸泡4h,充分溶胀,冰浴条件下进行60-80w超声处理5s停止15s,进行10次循环,在4℃下6500-7000rpm离心10min,取上清液,将硫酸铵以1-1.2mg/mL的添加量加入上清液中,30-60rpm搅拌1h,降温至4℃以9000-10000rpm离心8-10min,过滤冷冻干燥得到红藻藻胆蛋白;
S5、将S3所得硒化复合多糖与S4所得红藻藻胆蛋白混合均匀,得到多糖-蛋白混合物,将多糖-蛋白混合物加入水中,100-120rpm速度70-85℃水浴搅拌5-6min,得到多元改性复配凝胶。
2.根据权利要求1所述的含牡丹提取物的润肤抗皱面霜,其特征在于:在S2中,所述复合粉末在去离子水中的添加量为0.12-0.15g/mL。
3.根据权利要求2所述的含牡丹提取物的润肤抗皱面霜,其特征在于:在S2中,所述纤维素酶的添加量为复合粉末质量分数的2-3%;果胶酶的添加量为复合粉末质量分数的1-2%。
4.根据权利要求3所述的含牡丹提取物的润肤抗皱面霜,其特征在于:在S3中,所述牡丹-马尾藻多糖在硝酸溶液中的添加量为8-12mg/mL。
5.根据权利要求4所述的含牡丹提取物的润肤抗皱面霜,其特征在于:在S3中,所述亚硒酸钠在混合液中的添加量为10-15mg/mL。
6.根据权利要求5所述的含牡丹提取物的润肤抗皱面霜,其特征在于:在S4中,所述红藻粉末在去离子水中的添加量为60-80mg/mL。
7.根据权利要求6所述的含牡丹提取物的润肤抗皱面霜,其特征在于:在S4中,所述硫酸铵在上清液中的添加量为1-1.2mg/mL。
8.一种制备权利要求1所述的含牡丹提取物的润肤抗皱面霜的方法,其特征在于:具体包括如下步骤:将甘油、透明质酸、棕榈酸异丙酯、烟酰胺、泛酞醇、棕榈酰五肽-4和生育酚乙酸酯加入水中,搅拌均匀,然后加入多元改性复配凝胶,冰浴条件下,1000-1200rpm搅拌5-8min,混合均匀得到含牡丹提取物的润肤抗皱面霜。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202311767655.7A CN117427017B (zh) | 2023-12-21 | 2023-12-21 | 一种含牡丹提取物的润肤抗皱面霜及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202311767655.7A CN117427017B (zh) | 2023-12-21 | 2023-12-21 | 一种含牡丹提取物的润肤抗皱面霜及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN117427017A CN117427017A (zh) | 2024-01-23 |
CN117427017B true CN117427017B (zh) | 2024-03-05 |
Family
ID=89555776
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202311767655.7A Active CN117427017B (zh) | 2023-12-21 | 2023-12-21 | 一种含牡丹提取物的润肤抗皱面霜及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN117427017B (zh) |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008056643A (ja) * | 2006-09-04 | 2008-03-13 | Mie Univ | 抗酸化・細胞活性賦活剤及びこれを配合した化粧料 |
CN101917965A (zh) * | 2007-11-19 | 2010-12-15 | 施泰福实验室股份有限公司 | 局部化妆用皮肤增亮组合物及其使用方法 |
CN102300578A (zh) * | 2008-12-01 | 2011-12-28 | 延寿有限责任公司 | 用于改变健康、安康和寿命的方法和组合物 |
KR20120074498A (ko) * | 2010-12-28 | 2012-07-06 | 재단법인 경기과학기술진흥원 | 경단구슬모자반 추출물 또는 이로부터 분리한 유효물질을 이용한 알러지성 질환의 개선제 조성물 |
CN105819998A (zh) * | 2016-05-17 | 2016-08-03 | 阜阳大丰收农业种植专业合作社 | 一种增强油牡丹光合作用的液态培养基质及其制备方法 |
CN106491659A (zh) * | 2016-10-13 | 2017-03-15 | 暨南大学 | 一种抗肠炎螺旋藻硒化藻胆蛋白多肽复合体及其制备与应用 |
CN106714544A (zh) * | 2014-07-03 | 2017-05-24 | 埃特比公司 | 一定质地的可摄取产品 |
CN114213512A (zh) * | 2021-12-21 | 2022-03-22 | 佛山蓝强生物科技有限公司 | 增强藻胆蛋白光热稳定性的组合物及其制备方法与应用 |
CN117045564A (zh) * | 2023-10-11 | 2023-11-14 | 北京世纪伟信医药科技有限公司 | 一种含牡丹提取物的护肤品及其制备方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060018867A1 (en) * | 2004-05-12 | 2006-01-26 | Ichimaru Pharcos Co., Ltd | Cosmetic composition and production thereof |
-
2023
- 2023-12-21 CN CN202311767655.7A patent/CN117427017B/zh active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008056643A (ja) * | 2006-09-04 | 2008-03-13 | Mie Univ | 抗酸化・細胞活性賦活剤及びこれを配合した化粧料 |
CN101917965A (zh) * | 2007-11-19 | 2010-12-15 | 施泰福实验室股份有限公司 | 局部化妆用皮肤增亮组合物及其使用方法 |
CN102300578A (zh) * | 2008-12-01 | 2011-12-28 | 延寿有限责任公司 | 用于改变健康、安康和寿命的方法和组合物 |
KR20120074498A (ko) * | 2010-12-28 | 2012-07-06 | 재단법인 경기과학기술진흥원 | 경단구슬모자반 추출물 또는 이로부터 분리한 유효물질을 이용한 알러지성 질환의 개선제 조성물 |
CN106714544A (zh) * | 2014-07-03 | 2017-05-24 | 埃特比公司 | 一定质地的可摄取产品 |
CN105819998A (zh) * | 2016-05-17 | 2016-08-03 | 阜阳大丰收农业种植专业合作社 | 一种增强油牡丹光合作用的液态培养基质及其制备方法 |
CN106491659A (zh) * | 2016-10-13 | 2017-03-15 | 暨南大学 | 一种抗肠炎螺旋藻硒化藻胆蛋白多肽复合体及其制备与应用 |
CN114213512A (zh) * | 2021-12-21 | 2022-03-22 | 佛山蓝强生物科技有限公司 | 增强藻胆蛋白光热稳定性的组合物及其制备方法与应用 |
CN117045564A (zh) * | 2023-10-11 | 2023-11-14 | 北京世纪伟信医药科技有限公司 | 一种含牡丹提取物的护肤品及其制备方法 |
Non-Patent Citations (1)
Title |
---|
"化学修饰多糖的方法及生物活性研究进展";杨艺等;《食品工业科技》;20230630;第44卷(第11期);465-478 * |
Also Published As
Publication number | Publication date |
---|---|
CN117427017A (zh) | 2024-01-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109330911B (zh) | 一种含复合维生素和透明质酸盐的组合物及其应用 | |
CN103315931A (zh) | 黄秋葵提取液、黄秋葵提取物及其制备方法和应用 | |
CN108578266B (zh) | 一种抗氧化组合物及其在面膜液中的应用 | |
CN112807264B (zh) | 一种玻尿酸蓝铜胜肽组合物及其制备方法和应用 | |
CN116003577B (zh) | 一种用于皮肤舒缓修复的重组胶原蛋白及其应用 | |
CN105434319A (zh) | 一种基于红发夫酵母原料的化妆品及其制备方法 | |
CN109091422B (zh) | 含榆黄菇提取液的化妆品及其制备方法 | |
CN107411998B (zh) | 一种含带鱼活性肽的抗氧化面膜 | |
CN105687040B (zh) | 一种含海藻酶解物和胶原蛋白的多功能乳液及其制备方法 | |
CN108635275B (zh) | 一种抗氧化组合物及其在精华液中的应用 | |
CN117427017B (zh) | 一种含牡丹提取物的润肤抗皱面霜及其制备方法 | |
CN110833519A (zh) | 一种含工业大麻叶提取物的舒缓保湿面膜液及其制备方法 | |
CN101249059A (zh) | 一种保湿面膜及其制备方法 | |
CN108721192A (zh) | 一种天然植物提取精华面膜液及其提取工艺 | |
CN117257703A (zh) | 一种不含化学美白剂的美白组份 | |
CN111643392A (zh) | 一种具有多效美白抗敏作用的生物寡肽精华液及其制备方法 | |
CN110179728A (zh) | 一种可食用冻干粉制剂及其制备方法 | |
KR102381645B1 (ko) | 복령발효추출물을 포함하는 화장료 조성물 | |
CN115414289A (zh) | 虫草花化妆品及其制备方法 | |
CN116869856B (zh) | 一种含有超氧化物歧化酶的组合物及其应用 | |
RU2704490C1 (ru) | Композиция против выпадения и стимулирующая рост волос | |
CN108721186B (zh) | 一种抗衰面霜及其制备方法 | |
CN111840204A (zh) | 一种含有植物精华的抗衰老冻干粉及其制备方法 | |
CN115300443B (zh) | 一种羊胎素提取方法及在化妆品中的应用 | |
CN114796075B (zh) | 含铁皮石斛花的复合物及其在护肤产品中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |