CN117357431A - Seaweed polysaccharide composition and preparation method and application thereof - Google Patents
Seaweed polysaccharide composition and preparation method and application thereof Download PDFInfo
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- CN117357431A CN117357431A CN202311570988.0A CN202311570988A CN117357431A CN 117357431 A CN117357431 A CN 117357431A CN 202311570988 A CN202311570988 A CN 202311570988A CN 117357431 A CN117357431 A CN 117357431A
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- polysaccharide
- mixture
- enteromorpha
- solution
- cream
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- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 134
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 134
- 239000000203 mixture Substances 0.000 title claims abstract description 94
- 241001474374 Blennius Species 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 150000004676 glycans Chemical class 0.000 title claims abstract 28
- 241000196252 Ulva Species 0.000 claims abstract description 39
- DBTMGCOVALSLOR-DEVYUCJPSA-N (2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](CO)O[C@H](O)[C@@H]2O)O)O[C@H](CO)[C@H]1O DBTMGCOVALSLOR-DEVYUCJPSA-N 0.000 claims abstract description 23
- 229920001543 Laminarin Polymers 0.000 claims abstract description 23
- 239000005717 Laminarin Substances 0.000 claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 22
- 239000002537 cosmetic Substances 0.000 claims abstract description 20
- 241000195474 Sargassum Species 0.000 claims abstract description 16
- 229940023569 palmate Drugs 0.000 claims abstract description 8
- 239000006071 cream Substances 0.000 claims description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 23
- 239000000843 powder Substances 0.000 claims description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 18
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 claims description 16
- -1 polydimethylsiloxane Polymers 0.000 claims description 15
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims description 10
- 229960005323 phenoxyethanol Drugs 0.000 claims description 10
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 9
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 9
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 9
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 9
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 9
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims description 8
- 229920002498 Beta-glucan Polymers 0.000 claims description 8
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 8
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 8
- 239000010495 camellia oil Substances 0.000 claims description 8
- 238000009472 formulation Methods 0.000 claims description 8
- XUGNVMKQXJXZCD-UHFFFAOYSA-N isopropyl palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC(C)C XUGNVMKQXJXZCD-UHFFFAOYSA-N 0.000 claims description 8
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 claims description 8
- 229940032094 squalane Drugs 0.000 claims description 8
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- LADGBHLMCUINGV-UHFFFAOYSA-N tricaprin Chemical compound CCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCC)COC(=O)CCCCCCCCC LADGBHLMCUINGV-UHFFFAOYSA-N 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
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- 229940075507 glyceryl monostearate Drugs 0.000 claims description 6
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- 238000000034 method Methods 0.000 claims description 5
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 claims description 5
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- VLPFTAMPNXLGLX-UHFFFAOYSA-N trioctanoin Chemical compound CCCCCCCC(=O)OCC(OC(=O)CCCCCCC)COC(=O)CCCCCCC VLPFTAMPNXLGLX-UHFFFAOYSA-N 0.000 claims description 3
- 150000001298 alcohols Chemical class 0.000 claims description 2
- YQEMORVAKMFKLG-UHFFFAOYSA-N glycerine monostearate Natural products CCCCCCCCCCCCCCCCCC(=O)OC(CO)CO YQEMORVAKMFKLG-UHFFFAOYSA-N 0.000 claims description 2
- SVUQHVRAGMNPLW-UHFFFAOYSA-N glycerol monostearate Natural products CCCCCCCCCCCCCCCCC(=O)OCC(O)CO SVUQHVRAGMNPLW-UHFFFAOYSA-N 0.000 claims description 2
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- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 241000196253 Ulva prolifera Species 0.000 description 2
- 241001148683 Zostera marina Species 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
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- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 2
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
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- 241001466453 Laminaria Species 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
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- 241001248610 Ophiocordyceps sinensis Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 241000220690 Sargassum pallidum Species 0.000 description 1
- 240000006023 Trichosanthes kirilowii Species 0.000 description 1
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- 239000000783 alginic acid Substances 0.000 description 1
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- 238000004113 cell culture Methods 0.000 description 1
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- 230000019522 cellular metabolic process Effects 0.000 description 1
- 229940106189 ceramide Drugs 0.000 description 1
- 150000001783 ceramides Chemical class 0.000 description 1
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- 229920001436 collagen Polymers 0.000 description 1
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- 230000008588 hemolysis Effects 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
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- 150000002632 lipids Chemical class 0.000 description 1
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- 229960002446 octanoic acid Drugs 0.000 description 1
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- 150000002989 phenols Chemical class 0.000 description 1
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- 239000000419 plant extract Substances 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
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- 239000003223 protective agent Substances 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Sustainable Development (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Epidemiology (AREA)
- Molecular Biology (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to a seaweed polysaccharide composition, a preparation method and application thereof. The seaweed polysaccharide composition comprises sargassum polysaccharide, enteromorpha polysaccharide and laminarin palmata; respectively preparing a gulfweed polysaccharide, an enteromorpha polysaccharide and laminarin palmate into solutions, and then mixing. The seaweed polysaccharide composition has stable physical properties and no irritation when applied to skin care products; the preparation method is simple, has obvious efficacy and has good application prospect.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a seaweed polysaccharide composition, a preparation method and application thereof.
Background
Skin is a natural barrier of the human body, and moisture absorption and retention are key factors for maintaining the normal function of the barrier. Healthy skin is smooth, soft and elastic, and the moisture content in the stratum corneum is the most important factor in maintaining skin softness and elasticity. Only when the moisture is sufficient, the cells in the skin tissue can perform normal metabolism, maintain normal tissue structure and physiological function, resist all adverse factors such as sunlight irradiation, chemical substance stimulation and the like, and can keep the nutrition and the moisture in the cells sufficient, thereby ensuring the stability of the environment in the organism and ensuring the moist and glossy skin. Most of the cosmetics sold in the market are supplemented with important oily components and hydrophilic moisturizing components, and are taken as carriers to bring other active components into the cosmetics to achieve the purpose of moisturizing, so that the skin is moisturized and healthy. The moisturizing materials commonly used in cosmetics at present mainly include polyols (propylene glycol and the like), polysaccharides (hyaluronic acid and the like) and ceramides.
The seaweed polysaccharide is one of seaweed bioactive substances, and has various biological activities such as immunoregulation, antivirus, antioxidation, etc.
Gulfweed (Sargassum pallidum), known as "kelp", is considered one of the classic edible and medicinal brown algae. The parllidum has been shown to contain a variety of chemicals such as phenols, triterpenes and polysaccharides. Polysaccharides as the main active ingredient have been attracting increasing research interest due to their various beneficial effects on the human body.
Enteromorpha prolifera is a large-scale economic green alga and is rich in various physiologically active components such as polysaccharide, protein, vitamin, mineral and the like. Modern researches have shown that enteromorpha polysaccharide has good biological activities of reducing blood lipid, resisting virus, improving immunity and the like.
The kelp is brown algae, contains alginic acid, polysaccharide, vitamins, minerals and other components, promotes repair of damaged tissues, improves cell metabolism, stimulates collagen synthesis, enhances skin water-locking capacity and permeability, improves skin color darkness, reduces wrinkles, consolidates hair bulbs and prevents hair from falling off, and can be used for repairing, anti-wrinkle, hair care and other products.
Chinese patent application 201410056366.0 discloses Enteromorpha polysaccharide as human skin fibroblast protectant in cosmetics; when the enteromorpha polysaccharide is used as a human skin fibroblast protective agent, the mass concentration of the added amount of the enteromorpha polysaccharide is 0.2-0.8%. Experimental researches show that enteromorpha polysaccharide has good moisture absorption and moisture retention activities, has a good protection effect on skin cells, and particularly has obviously improved cell survival rate under a dry condition, so that the enteromorpha polysaccharide can be used as a moisture retention and moisture absorption agent in cosmetics. But the invention does not achieve the effect of simultaneously moisturizing, soothing and resisting oxidation.
The Chinese patent application 202210968368.1 discloses a moisturizing, antioxidant and soothing composition which comprises the following components in parts by weight: 0.1-2 parts of Cordyceps sinensis extract; 0.05-1 part of wisteria extract; 0.03-1.2 parts of radix peucedani extract; 0.06-1.5 parts of trichosanthes kirilowii Maxim extract; 0.08-1.3 parts of willow coral extract; 0.05-0.5 parts of hexyl glucoside. The facial mask obtained by the composition has good effects of keeping moisture, resisting oxidation and wrinkle and relieving inflammation, but has complex components and higher cost.
Cosmetics have become an indispensable product, and cosmetics containing natural plant extracts have been receiving increasing attention. However, some cosmetics are very unfavorable to the health of people after long-term use, and can cause skin relaxation, edema, excessive thin horny layer, easy allergy and the like, and especially, the existing products are commonly added with an activating component to promote the skin to absorb nutrition components, so that the skin problem can be temporarily relieved, but the skin becomes more fragile and easy to age after long-term use. Most of the skin care products used at present have the problems of undefined effect, adverse reaction and the like, so people need to find a product with various effects, safety, no adverse reaction and definite effect and having the effects of moisturizing, relieving and resisting oxidation. Meanwhile, the skin care product with moisturizing, relieving and antioxidant functions and various dosage forms such as water aqua, emulsion, cream and essence are lacking in the market.
Disclosure of Invention
In order to solve the problems, the invention provides a seaweed polysaccharide composition, a preparation method and application thereof. The natural plant source compound composition obtained by the invention has stable properties, is non-irritating when applied to skin care products, and has the effects of relieving, moisturizing and resisting oxidation.
In order to achieve the above purpose of the present invention, the present invention adopts the following specific technical scheme:
a algal polysaccharide composition comprising sargassum polysaccharide, enteromorpha polysaccharide, and laminarin palmata.
Preferably, the mass ratio of the gulfweed polysaccharide, the enteromorpha polysaccharide and the laminarin palmata is 2-3:1-2:2-3.
Preferably, the preparation method of gulfweed polysaccharide, enteromorpha polysaccharide and laminarin palmata comprises the following steps: respectively collecting Sargassum powder, enteromorpha powder and herba Zosterae Marinae powder, extracting with water by distillation, concentrating, washing, and lyophilizing.
Preferably, the water distillation extraction comprises: adding 30-50 times of water into Sargassum powder, enteromorpha powder and herba Zosterae Marinae powder, respectively, reflux extracting in water bath at 85-95deg.C for 2-4 hr for 2-3 times.
Further preferably, the lyophilization temperature is 0-4 ℃ and the lyophilization time is 24-48 hours.
The invention also relates to a preparation method of the seaweed polysaccharide composition, which comprises the following steps: the gulfweed polysaccharide, the enteromorpha polysaccharide and the palmate laminarin are respectively prepared into a gulfweed polysaccharide solution, an enteromorpha polysaccharide solution and a palmate laminarin solution, and then the gulfweed polysaccharide solution, the enteromorpha polysaccharide solution and the palmate laminarin solution are mixed.
Preferably, the concentration of the gulfweed polysaccharide solution and the enteromorpha polysaccharide solution laminarin solution is 1.5-1.7mg/mL, and the volume ratio of the mixture is 2-3:1-2:2-3.
The invention also relates to application of the seaweed polysaccharide composition in preparing cosmetics.
Preferably, the seaweed polysaccharide composition has a mass of 0.1% -5% of the total mass of the cosmetic formulation.
Further preferably, the algal polysaccharide composition has a mass of 4% -5% of the total mass of the cosmetic formulation.
Preferably, the cosmetic further comprises an auxiliary material, wherein the auxiliary material comprises one or more of water, a humectant, a conditioner, a thickener, a preservative and essence.
Preferably, the cosmetic is formulated into cream, emulsion, essence, or facial mask.
Further preferably, the cosmetic is a cream, and the raw materials of the cream include 16 alcohol or 18 alcohol, tea seed oil, squalane, glyceryl monostearate, PEG-100 stearate, polyglycerol-3-methylglucdistearate, caprylic or capric triglyceride, isopropyl palmitate, polydimethylsiloxane, EDTA-2Na, carbopol 940, glycerol, trehalose, triethanolamine, beta-glucan, phenoxyethanol, algal polysaccharide composition, and water.
Further preferably, the cream comprises, in mass percent, 2.5% -3.5% of 16 or 18 alcohols, 0.8% -1.2% of tea seed oil, 2.5% -3.5% of squalane, 0.8% -1.2% of glycerol monostearate, 0.8% -1.2% of PEG-100 stearate, 1.0% -1.5% of polyglycerol-3-methylglucdistearate, 2.5% -3.5% of caprylic acid or capric acid triglyceride, 2.5% -3.5% of isopropyl palmitate, 2.5% -3.5% of polydimethylsiloxane, 0.04% -0.06% of EDTA-2Na, 0.05% -0.15% of carbopol 940, 4% -6% of glycerol, 0.04% -0.06% of trehalose, 0.08% -0.12% of triethanolamine, 1.2% -1.8% of beta-glucan, 0.4% -0.6% of phenoxyethanol, and the balance of water.
Further preferably, the preparation method of the cream comprises the following steps:
(1) Mixing 16 alcohol or 18 alcohol, tea seed oil, squalane, glyceryl monostearate, PEG-100 stearate, polyglycerol-3-methyl glucose distearate, caprylic acid triglyceride or capric acid triglyceride, isopropyl palmitate, and polydimethylsiloxane to obtain mixture A;
(2) Mixing EDTA-2Na, carbopol 940, glycerol and water to obtain a mixture B;
(3) Mixing trehalose, triethanolamine and water to obtain a mixture C;
(4) Mixing the seaweed polysaccharide composition with beta-glucan to obtain a mixture D;
(5) Mixing the mixture A and the mixture B, homogenizing, cooling, and adding the mixture C, the mixture D and phenoxyethanol.
Preferably, the temperature of the mixture in the step (5) is 80-85 ℃, the temperature of the mixture C and the mixture D is 50-55 ℃, and the temperature of the phenoxyethanol is 40-45 ℃.
Compared with the prior art, the invention has the beneficial effects that:
(1) The seaweed polysaccharide composition has stable properties, is non-irritating when applied to skin care products, and has the effects of relieving, moisturizing and resisting oxidation;
(2) The preparation method is simple, has obvious efficacy and has good application prospect.
Drawings
FIG. 1 is a graph showing the effect of the cream prepared in example 1 of the present invention on RAW264.7 cytotoxicity;
FIG. 2 is a graph showing the effect of the cream of example 1 of the present invention on the amount of NO released from RAW264.7 cells;
FIG. 3 is a graph showing the effect of the cream of example 1 of the present invention on HaCaT cytotoxicity;
FIG. 4 is a graph showing the experimental results of the protection of HaCaT cells from oxidative damage by the cream prepared in example 1 of the present invention;
FIG. 5 is a graph showing the effect of the cream of example 1 of the present invention on the amount of ROS released in HaCaT cells;
note that: * P＜0.05、 ** P＜0.01、 *** P＜0.001、 **** P＜0.0001； * indicating that the composition was significantly different from the model group at this concentration.
Detailed Description
The technical solutions of the embodiments of the present invention are further clearly described, and the described embodiments are only a part of the present invention, which are used to explain the present invention, but not to limit the present invention, so that other embodiments obtained by other persons skilled in the art without creative efforts fall within the protection scope of the present invention.
Polydimethylsiloxane, product number D849784-100g; carbopol 940, cat No. C832684-500g; trehalose, product number D964252-500g; other raw materials in the cream formula are purchased from Shanghai Meilin Biochemical technology Co., ltd;
HaCaT cells, RAW264.7 cells, DMEM high-sugar medium were purchased from North Nanophyte.
The reagents not specifically described are all commonly used reagents and can be purchased from conventional reagent manufacturing and selling companies.
Example 1
1. A seaweed polysaccharide composition comprises Sargassum polysaccharide, enteromorpha polysaccharide and laminarin at a mass ratio of 2:1:2.
The preparation method of the seaweed polysaccharide composition comprises the following steps: collecting Sargassum powder, enteromorpha powder and herba Zosterae Marinae powder, adding 40 times of distilled water, reflux extracting in water bath at 90deg.C for 3 hr for 2 times, centrifuging the extractive solution (2000 r/min,10 min), and collecting supernatant. Concentrating the supernatant by a rotary evaporator until the solution is thick, adding 4 times of ethanol with the mass concentration of 90%, freezing and precipitating for 24 hours at the temperature of 4 ℃, washing the precipitate, and drying the precipitate by a freeze dryer for 24 hours to obtain crude polysaccharide of gulfweed, crude polysaccharide of enteromorpha and crude polysaccharide of kelp.
Preparing three kinds of algal crude polysaccharide into algal polysaccharide solutions with mass concentration of 1.6mg/mL respectively, and preparing gulfweed polysaccharide solutions: enteromorpha polysaccharide solution: the laminarin solution is prepared into polysaccharide compound solution according to the volume ratio of 2:1:2.
2. The raw material formula and the dosage of the cream containing the seaweed polysaccharide composition are shown in Table 1:
table 1 cream formulation
3. A preparation method of cream containing the algal polysaccharide composition comprises the following steps:
(1) Mixing 18 alcohol, tea seed oil, squalane, glyceryl monostearate, glyceryl stearate, polyglycerol-3-methyl glucose distearate, capric acid triglyceride, isopropyl palmitate and polydimethylsiloxane according to the formula amount to obtain a mixture A;
(2) Mixing EDTA-2Na, carbopol 940, glycerol and pure water according to the formula amount to obtain a mixture B;
(3) Mixing trehalose, triethanolamine and pure water according to the formula amount to obtain a mixture C;
(4) Mixing the beta-glucan and the seaweed polysaccharide composition in a formula amount to obtain a mixture D;
(5) Heating the mixture A and the mixture B to 85 ℃, mixing, homogenizing, cooling to 55 ℃, and adding the mixture C and the mixture D while stirring; cooling to 45deg.C, adding phenoxyethanol under stirring, and stirring at room temperature.
Example 2
1. A seaweed polysaccharide composition comprises Sargassum polysaccharide, enteromorpha polysaccharide and laminarin at a mass ratio of 2.5:1.5:2.5.
The preparation method of the seaweed polysaccharide composition comprises the following steps: collecting Sargassum powder, enteromorpha powder and herba Zosterae Marinae powder, adding 30 times of distilled water, reflux extracting in water bath at 85deg.C for 4 hr for 2 times, centrifuging the extractive solution (2000 r/min,10 min), and collecting supernatant. The supernatant was concentrated to a solution viscosity by rotary evaporator. Adding 4 times of 90% ethanol, freeze precipitating at 4deg.C for 24 hr, washing, and lyophilizing for 48 hr to obtain Sargassum crude polysaccharide, enteromorpha crude polysaccharide, and Zostera Marina crude polysaccharide.
Preparing three kinds of algal crude polysaccharide into algal polysaccharide solutions with mass concentration of 1.5mg/mL respectively, and preparing gulfweed polysaccharide solutions: enteromorpha polysaccharide solution: the laminarin solution is prepared into polysaccharide compound solution according to the volume ratio of 2.5:1.5:2.5.
2. The raw material formula and the dosage of the cream containing the seaweed polysaccharide composition are shown in Table 2:
table 2 cream formulation
3. A preparation method of cream containing the algal polysaccharide composition comprises the following steps:
(1) Mixing 16 alcohol, tea seed oil, squalane, glyceryl monostearate, glyceryl stearate, polyglycerol-3-methyl glucose distearate, caprylic acid triglyceride, isopropyl palmitate and polydimethylsiloxane according to the formula amount to obtain a mixture A;
(2) Mixing EDTA-2Na, carbopol 940, glycerol and pure water according to the formula amount to obtain a mixture B;
(3) Mixing trehalose, triethanolamine and pure water according to the formula amount to obtain a mixture C;
(4) Mixing the beta-glucan and the seaweed polysaccharide composition in a formula amount to obtain a mixture D;
(5) Heating the mixture A and the mixture B to 80 ℃, mixing, homogenizing, cooling to 50 ℃, and adding the mixture C and the mixture D while stirring; cooling to 40deg.C, adding phenoxyethanol under stirring, and stirring at room temperature.
Example 3
1. A seaweed polysaccharide composition comprises Sargassum polysaccharide, enteromorpha polysaccharide and laminarin at a mass ratio of 3:2:3.
The preparation method of the seaweed polysaccharide composition comprises the following steps: collecting Sargassum powder, enteromorpha powder and herba Zosterae Marinae powder, adding 50 times of distilled water, reflux extracting in 95deg.C water bath for 2 hr for 3 times, centrifuging the extractive solution (2000 r/min,10 min), and collecting supernatant. The supernatant was concentrated to a solution viscosity by rotary evaporator. Adding 4 times of 90% ethanol, freeze precipitating at 4deg.C for 24 hr, washing, and lyophilizing for 30 hr to obtain Sargassum crude polysaccharide, enteromorpha crude polysaccharide, and Zostera Marina crude polysaccharide.
Preparing three kinds of algal crude polysaccharide into algal polysaccharide solutions with mass concentration of 1.7mg/mL respectively, and preparing gulfweed polysaccharide solutions: enteromorpha polysaccharide solution: the laminarin solution is prepared into polysaccharide compound solution according to the volume ratio of 3:2:3.
2. A cream containing the algal polysaccharide composition has the raw material formula and the raw material amount shown in Table 3.
Table 3 cream formulation
3. A method for preparing a cream containing the algal polysaccharide composition is consistent with example 1.
Comparative example 1
1. A seaweed polysaccharide composition comprises Sargassum polysaccharide and laminarin at a mass ratio of 3:2.
The preparation method of the seaweed polysaccharide composition comprises the following steps: collecting Sargassum powder and Sargassum palmatum powder, adding 40 times of distilled water, reflux-extracting in water bath at 90deg.C for 3 hr for 2 times, centrifuging the extractive solution (2000 r/min,10 min), and collecting supernatant. Concentrating the supernatant by a rotary evaporator until the solution is viscous, adding 4 times of ethanol with the mass concentration of 90%, freezing and precipitating at the temperature of 24 hours and 4 ℃, washing the precipitate, and drying the precipitate by a freeze dryer for 24 hours to obtain crude polysaccharide of gulfweed and crude polysaccharide of kelp.
Preparing algae polysaccharide solution with mass concentration of 1.6mg/mL from crude gulfweed polysaccharide and crude kelp polysaccharide respectively, and preparing polysaccharide compound solution from crude gulfweed polysaccharide and crude kelp polysaccharide according to volume ratio of 3:2.
2. A cream containing the algal polysaccharide composition, the formulation, the amount and the preparation method are the same as those of example 1.
Comparative example 2
1. A seaweed polysaccharide composition comprises enteromorpha polysaccharide and laminarin in a mass ratio of 3:2.
The preparation method of the seaweed polysaccharide composition comprises the following steps: respectively collecting Enteromorpha prolifera powder and herba Zosterae Marinae powder, adding 40 times of distilled water, reflux extracting in water bath at 90deg.C for 3 hr for 2 times, centrifuging the extractive solution (2000 r/min,10 min), and collecting supernatant. Concentrating the supernatant by a rotary evaporator until the solution is viscous, adding 4 times of ethanol with the mass concentration of 90%, freezing and precipitating for 24 hours at the temperature of 4 ℃, washing the precipitate, and drying the precipitate by a freeze dryer for 24 hours to obtain the enteromorpha crude polysaccharide and the palmate laminaria crude polysaccharide.
Preparing an algae polysaccharide solution with the mass concentration of 1.6mg/mL from the enteromorpha crude polysaccharide and the kelp crude polysaccharide respectively, and preparing a polysaccharide compound solution from the gulfweed crude polysaccharide solution and the kelp crude polysaccharide solution according to the volume ratio of 3:2.
2. A cream containing the algal polysaccharide composition, the formulation, the amount and the preparation method are the same as those of example 1.
Test example 1 mouse phagocyte RAW264.7 Activity assay
1. The experimental method comprises the following steps: RAW264.7 cells grown in log phase were grown at 2X 10 4 Inoculating 100 mu L of each hole into a 96-well plate, culturing for 24 hours in an incubator, adding a cream blank group with different mass concentrations into a DMEM high-sugar culture medium, continuing for 24 hours in the incubator, then adding 10 mu L of MTT solution with the concentration of 5mg/mL, incubating for 4 hours, sucking out the culture medium, adding 100 mu L of DMSO, measuring absorbance at 490nm by using an enzyme-labeled instrument, and calculating the cell survival rate according to a formula.
2. The formula:
3. experimental results
As shown in FIG. 1, the cream prepared in example 1 has NO obvious inhibition effect on the activity of RAW264.7 cells at a concentration of less than 10mg/mL, and the soothing cream is considered to be nontoxic or extremely low in toxicity and can be used for testing the influence on the release amount of NO in RAW264.7 cells.
Test example 2 determination of NO Release amount in mouse phagocytes RAW264.7
1. The experimental method comprises the following steps: log phase RAW264.7 cells were taken at 1×10 5 Each well was inoculated in a 96-well plate at 100. Mu.L/well, cultured in an incubator for 24 hours, and the supernatant was aspirated after the cells were completely adhered. Adding 100 mu L of algal polysaccharide solutions with different concentrations into the sample group; the positive control group was added with 100. Mu.L of Dexamethasone (DEX) solution at 0.2mg/mL, 100. Mu.L of LPS solution (1 mg/L) was added to each well except the blank group, 100. Mu.L of LPS solution was added to the LPS model group, the mixture was supplemented to 200. Mu.L with DMEM high-sugar medium, and the blank group was cultured for 24 hours with DMEM high-sugar medium alone, and then cultured in the cell incubator for 24 hours. Cell supernatants were taken and subjected to subsequent manipulations according to the NO kit (branded bi yun, cat No. s0021 s).
2. Experimental results
As shown in fig. 2, the cream prepared in example 1 has a significant inhibitory effect on release of NO in BRAW264.7 cells at a concentration of less than 5mg/mL and has a certain concentration dependence.
Test example 3 determination of human epidermal immortalized HaCaT cell Activity
1. The experimental method comprises the following steps: cells in the logarithmic growth phase were taken and tested. The digested HaCaT cells were then subjected to a 1X 10 protocol 5 Inoculating 100 μL of each well into 96-well plate, culturing in incubator for 24 hr, treating cells with compound cream containing different concentrations, incubating for 24 hr, adding 5mg/mL MTT100 μL, culturing in incubator for 4 hr, sucking off culture medium, adding 100 μL DMSO, and measuring absorbance at 490nm
2. The formula:
3. experimental results: as shown in FIG. 3, the soothing cream prepared in example 1 has no obvious inhibition effect on HaCaT cell activity at a concentration of less than 10mg/mL, and can be considered to be nontoxic or extremely low in toxicity, and can be used for HaCaT cell oxidative damage protection measurement and test of influence of intracellular ROS release amount.
Test example 4 human epidermis immortalized HaCaT cell oxidative damage protection assay
1. The experimental method comprises the following steps: haCaT cells were grown at a cell density of 1X 10 5 Each of the cells was inoculated into a 96-well plate at a concentration of 100. Mu.L per well, and cultured in a cell culture incubator for 24 hours. Dividing the experiment into a compound cream protection group H 2 O 2 Model group, blank group. Adding 100 mu L of compound cream with different mass concentrations into the cream protection group, and incubating for 1H, wherein the cream protection group and H 2 O 2 The damage model group is added with H 2 O 2 (final concentration 0.25 mmol/L) 100. Mu.L, the blank group was added with an equal volume of DMEM high-sugar medium. After 24h incubation, cell viability was determined by MTT method (cf. Test example 3).
2. The formula:
3. experimental results: as shown in FIG. 4, the soothing cream prepared in example 1 was effective against H at a concentration of less than 5mg/mL 2 O 2 The induced HaCaT cell oxidative damage has a certain protection effect and has a certain concentration dependence.
Test example 5 determination of ROS content in human epidermal immortalized HaCaT cells
1. The experimental method comprises the following steps: haCaT cells were grown at 1X 10 5 Cell density of each mL was inoculated in 96-well plates at 100. Mu.L per well, and blank groups, H, were established 2 O 2 Injury model group and compound cream group. After 24H incubation, the cream protection group is added with 100 mu L of compound cream with different mass concentrations, H 2 O 2 The model group and the cream protection group are added with H 2 O 2 100. Mu.L of DMEM high-glucose medium (final volume concentration of 0.25 mmol/L) was added to the blank group. After the treatment, DCFH-DA was added to each well at a final volume concentration of 10. Mu.g/mL, and the cells were culturedIncubate for 30min in the case, shake 1 every 10min for probe and cell contact fully, and pay attention to the whole light-proof operation. After the incubation, the cells were washed 3 times with a preheated blank medium and the fluorescence intensity in the cells was measured by a multifunctional microplate reader at an excitation wavelength of 488nm and an emission wavelength of 525 nm. The fluorescence intensity of the blank group was set to 100%, and the relative fluorescence intensities in the cells of the other groups were calculated as compared with the fluorescence intensity of the blank group.
2. Experimental results: as shown in FIG. 5, the cream prepared in example 1 was effective against H at a concentration of less than 5mg/mL 2 O 2 The induced release of ROS in HaCaT cells has a certain inhibition effect and a certain concentration dependence.
Test example 6 measurement of moisturizing Property of human body
And (3) moisture preservation effect test: 80 volunteers (40 men and 40 women) were selected and randomized into 8 groups of 10 persons each (5 men and 5 women). After the subjects cleaned the skin of the arms, they were wiped dry and then the creams of examples 1-3 and comparative examples 1-4 were applied separately. Skin moisture content test method: the moisture content of the arm was measured in an environment having a temperature of 25℃and a relative humidity of 50%, and the moisture content of the arm before and after the use of the cream was measured by using a model CM825 tester supplied by CK, germany, respectively, and the percutaneous water loss was measured by the TEWL method, the results were recorded, and an average value was obtained. The results are shown in Table 4.
TABLE 4 determination of skin moisture content and percutaneous Water loss
Test example 7 erythrocyte hemolysis assay
1. The experimental method comprises the following steps: taking 500 mu L of 2% rabbit red blood cell suspension, respectively adding samples with different concentrations (PBS for dissolution and dilution), setting a negative group mixed with physiological saline and 2% rabbit red blood cell suspension in equal volume, and setting a positive group mixed with 1% Sodium Dodecyl Sulfate (SDS) solution and 2% rabbit red blood cell suspension in equal volume. Three groups of the enzyme labeling device are arranged in parallel, the three groups of the enzyme labeling device are placed in a 37 ℃ incubator for static culture for 3 hours, and then are centrifuged (2000 r/min,5 min), 100 mu L of supernatant is taken and placed in a 96-well plate, and absorbance values of the enzyme labeling device at 540, 560 and 575nm are measured.
2. The formula:
3. experimental results: the DI value of the cream prepared in example 1 is greater than 100, and the cream belongs to the non-irritation grade and has good safety.
Test example 9 human body Patch test measurement
1. The experimental method comprises the following steps: the skin of the part to be measured was cleaned, and the formulated cream (0.025 mg) was added to the plaque tester. The control wells were left without any substance and the plaque tester with the sample was applied to the arm of the subject and held for 24h. Observations were made 30min after removal of the plaque tester (after disappearance of the indentation), 24h and 48h, respectively.
2. Experimental results: as can be seen from Table 5, the soothing creams prepared in examples 1 and 2 have no obvious allergic reaction to human body, low irritation and good safety. The evaluation criteria are shown in Table 6.
TABLE 5 test results for Compound cream Enclosed Patch for skin of examples 1-2
TABLE 6 skin Performance scoring criteria
Test example 10 stability test
The sensory and physicochemical properties of the formulated soothing creams prepared in examples 1-3 and comparative examples 1-2 were evaluated, and the evaluation criteria and results are shown in Table 7.
Table 7 evaluation of sensory and physicochemical Properties of the formulated soothing cream
The foregoing detailed description is directed to one of the possible embodiments of the present invention, which is not intended to limit the scope of the invention, but is to be accorded the full scope of all such equivalents and modifications so as not to depart from the scope of the invention.
Claims (10)
1. The seaweed polysaccharide composition is characterized by comprising, by weight, 2-3 parts of gulfweed polysaccharide, 1-2 parts of enteromorpha polysaccharide and 2-3 parts of laminarin palmata.
2. The algal polysaccharide composition according to claim 1, wherein the gulfweed polysaccharide, the enteromorpha polysaccharide and the laminarin palmata are obtained by water distillation extraction, concentration, washing and freeze-drying; the water distillation extraction comprises: adding 30-50 times of water into Sargassum powder, enteromorpha powder and herba Zosterae Marinae powder, respectively, reflux extracting in water bath at 85-95deg.C for 2-4 hr for 2-3 times; the freeze-drying temperature is 0-4 ℃, and the freeze-drying time is 24-48h.
3. A process for the preparation of the algal polysaccharide composition according to any one of claims 1-2, comprising the steps of: the gulfweed polysaccharide, the enteromorpha polysaccharide and the palmate laminarin are respectively prepared into a gulfweed polysaccharide solution, an enteromorpha polysaccharide solution and a palmate laminarin solution, and then the gulfweed polysaccharide solution, the enteromorpha polysaccharide solution and the palmate laminarin solution are mixed.
4. The preparation method according to claim 3, wherein the concentration of the gulfweed polysaccharide solution and the enteromorpha polysaccharide solution laminarin solution is 1.5-1.7mg/mL, and the volume ratio of the mixture is 2-3:1-2:2-3.
5. Use of the algal polysaccharide composition of any one of claims 1-4 in the preparation of cosmetics.
6. The use according to claim 5, wherein the algal polysaccharide composition is added in an amount of 0.1% -5% of the total mass of the cosmetic formulation.
7. The use according to claim 5, wherein the cosmetic is formulated as a cream, emulsion, serum or mask, the cosmetic is a cream, and the raw materials of the cream include 16 alcohol or 18 alcohol, tea seed oil, squalane, glyceryl monostearate, PEG-100 stearate, polyglyceryl-3-methylglucdistearate, caprylic or capric triglyceride, isopropyl palmitate, polydimethylsiloxane, EDTA-2Na, carbo 940, glycerin, trehalose, triethanolamine, β -glucan, phenoxyethanol, algal polysaccharide composition and water.
8. Use according to claim 7, characterized in that the cream comprises, in mass%, from 2.5% to 3.5% of 16 or 18 alcohols, from 0.8% to 1.2% of tea seed oil, from 2.5% to 3.5% of squalane, from 0.8% to 1.2% of glycerol monostearate, from 0.8% to 1.2% of PEG-100, from 1.0% to 1.5% of polyglycerol-3-methylglucdistearate, from 2.5% to 3.5% of caprylic or capric triglyceride, from 2.5% to 3.5% of isopropyl palmitate, from 2.5% to 3.5% of polydimethylsiloxane, from 0.04% to 0.06% of EDTA-2Na, from 0.05% to 0.15% of carbo940, from 4% to 6% of glycerol, from 0.04% to 0.06% of trehalose, from 0.08% to 0.12% of triethanolamine, from 1.2% to 1.8% of β -glucan, from 0.6% of phenoxyethanol, and the balance of a combination of polysaccharides.
9. The use according to claim 8, characterized in that the method for preparing the cream comprises the following steps:
(1) Mixing 16 alcohol or 18 alcohol, tea seed oil, squalane, glyceryl monostearate, PEG-100 stearate, polyglycerol-3-methyl glucose distearate, caprylic acid triglyceride or capric acid triglyceride, isopropyl palmitate, and polydimethylsiloxane to obtain mixture A;
(2) Mixing EDTA-2Na, carbopol 940, glycerol and water to obtain a mixture B;
(3) Mixing trehalose, triethanolamine and water to obtain a mixture C;
(4) Mixing the seaweed polysaccharide composition with beta-glucan to obtain a mixture D;
(5) Mixing the mixture A and the mixture B, homogenizing, cooling, and adding the mixture C, the mixture D and phenoxyethanol.
10. The use according to claim 9, wherein the temperature of the mixing in step (5) is 80-85 ℃, the temperature of the addition of mixture C, mixture D is 50-55 ℃, and the temperature of the addition of phenoxyethanol is 40-45 ℃.
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