CN110772454A - Compound essential oil for skin brightening, moisturizing, soothing and anti-aging, and preparation method and application thereof - Google Patents

Compound essential oil for skin brightening, moisturizing, soothing and anti-aging, and preparation method and application thereof Download PDF

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Publication number
CN110772454A
CN110772454A CN201911289109.0A CN201911289109A CN110772454A CN 110772454 A CN110772454 A CN 110772454A CN 201911289109 A CN201911289109 A CN 201911289109A CN 110772454 A CN110772454 A CN 110772454A
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essential oil
oil
skin
moisturizing
lavender
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CN110772454B (en
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符春丽
田伟兰
汪垠明
汪羿典
祝荣梅
卢正美
汪翔
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Hubei Chuangjie Biotechnology Co Ltd
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Hubei Chuangjie Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/524—Preservatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

Abstract

The invention provides compound essential oil, which combines the theory of traditional Chinese medicine, the selected raw materials are sweet and flat in taste, and the good effects of removing black and brightening skin, resisting allergy, relieving, moisturizing and resisting aging are achieved through compounding, mutual blending, mutual matching and synergistic action of rosa damascena flower oil, lavender essential oil, jojoba seed oil, sweet almond oil, rosa canina fruit oil and wheat germ oil. The compound essential oil provided by the invention takes plant essential oil as a raw material, and solves the problems of low solubility, poor color, difficult absorption by skin and safety in the application of the compound essential oil in cosmetics; the invention improves the stability of the effective components and obviously improves the skin care effects of anti-allergy relieving, anti-aging whitening, moisturizing and moisturizing. The compound essential oil provided by the invention has multiple effects of skin brightening, soothing and repairing, skin moistening, aging resistance and the like, so that the compound essential oil has a good market prospect.

Description

Compound essential oil for skin brightening, moisturizing, soothing and anti-aging, and preparation method and application thereof
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to compound essential oil with skin brightening, moisturizing, soothing and anti-aging effects, and a preparation method and application thereof.
Background
Due to the influence of adverse factors such as air pollution, wide use of electronic products, radiation, staying up all night and the like, various problems can occur to endocrine of people, and the problems of darkness, acne, sensitivity, roughness and the like can occur to the skin. Skin care products in the prior art have a plurality of effects of whitening, spot lightening, anti-allergy, moisturizing and the like, but are mostly obtained by compounding effective substances and other auxiliary agents, wherein the effective substances are low in content on one hand, and most of the effective substances are large in molecular weight and difficult to absorb by skin on the other hand.
The essential oil is extracted from leaves, flowers, seeds, fruits, roots, barks, resins, hearts and other parts of plants by steam distillation, cold pressing, fat absorption and solvent extraction, and has high-concentration fragrance and volatility. Essential oils are composed of a few very small molecules, usually short in their molecular chains, which make them very permeable to the skin and enter the body via the abundant capillaries under the subcutaneous fat. The single-component essential oil has single effect, and the compound essential oils can supplement each other through mutual coordination and enhance the curative effect. However, since the research on the complex essential oil is not deep at home and abroad, the single essential oil with different efficacies is generally simply combined in the prior art. However, the compound essential oil has the problems of unobvious efficacy, low solubility, poor product color, difficulty in skin absorption, low safety performance due to the need of additionally adding preservatives and the like.
Disclosure of Invention
In order to solve the technical problems, the invention provides the compound essential oil comprising a plurality of components such as base essential oil, functional essential oil and the like, wherein the compound essential oil is combined with the cold and heat properties of the essential oil, is used for harmonizing the balance of yin and yang of a human body and is adjusted from inside to outside, so that the effect of improving the skin state from inside to outside is achieved, and the compound essential oil has the outstanding effects of whitening and brightening the skin, relieving and sensitizing, and replenishing water and moisturizing.
The invention aims to provide the compound essential oil for skin brightening, moisturizing, soothing and anti-aging.
The invention also aims to provide a preparation method of the compound essential oil for skin brightening, moisturizing, soothing and anti-aging.
The invention further aims to provide application of the compound essential oil for brightening skin, moisturizing, relieving and resisting aging.
According to the purpose of the invention, the invention provides a skin-brightening, moisturizing, soothing and anti-aging compound essential oil which comprises the following components in parts by weight:
0.1-15 parts of rosa damascena flower oil;
0.1-40 parts of lavender essential oil;
1-50 parts of jojoba seed oil;
1-50 parts of sweet almond oil;
0.1-50 parts of rosa canina fruit oil;
1-40 parts of wheat germ oil.
The compound essential oil provided by the invention is prepared by compounding the rosa damascena flower oil, the lavender essential oil, the jojoba seed oil, the sweet almond oil, the rosa canina fruit oil and the wheat germ oil, and combining the theory of traditional Chinese medicine, the selected raw materials are sweet and flat in taste, wherein the rosa damascena flower oil and the lavender essential oil are mainly used, the jojoba oil, the sweet almond oil and the rosa canina fruit oil are used as base oil, and the wheat germ oil serving as a plant preservative is added, so that the compound essential oil is matched with each other and has a synergistic effect, and the good effects of black dispelling, skin brightening, allergy resistance, relaxation, water replenishing and aging resistance are achieved.
The compound essential oil provided by the invention contains lavender essential oil and wheat germ oil with a certain antibacterial effect, and the lavender essential oil and the wheat germ oil interact with each other, and also has the effects of improving the stability and the corrosion resistance of the compound essential oil. The compound essential oil provided by the invention is safe and reliable in components, and can be kept for 3 years without deterioration under the condition of no addition of other preservatives.
In order to better explain the present invention, the efficacy of each component is provided below.
The Rosa damascena flower oil contains vitamin C, triterpenes, fructose, organic acid, citronellol, geraniol, etc., and also contains various amino acids and trace elements. Has effects of relieving pressure, removing scar, resisting allergy, whitening skin, regulating heart, stomach, liver and uterus, promoting blood circulation for removing blood stasis, relieving swelling and pain, and caring skin. Rosa damascena flower is oily and sweet, and flat and enters stomach and large intestine meridians.
The lavender essential oil contains linalool oxide, trans-linalool oxide, linalool acetate, lavender ester acetate, lavender alcohol, borneol and the like, and has the effects of calming, hypnotizing, resisting depression, resisting bacteria, clearing away heat and toxic materials, cleaning skin, controlling oil content, removing freckles, whitening, removing wrinkles, tendering skin, removing black eye circles in under-eye bags, promoting regeneration of damaged tissues and the like.
Jojoba seed oil, also known as jojoba essential oil, is extracted from jojoba beans; jojoba seed oil is rich in vitamin D and protein, is good moistening and moisturizing oil, can maintain skin moisture, prevent wrinkle and soften skin, is suitable for mature and aged skin, and is used for face and body massage and hair care.
Sweet almond is sweet in flavor and neutral in nature, and enters lung and large intestine meridians. Sweet almond oil, which contains abundant vitamins and is easy to absorb, has the effects of nourishing, moisturizing, relieving and anti-allergy, and can promote cell growth; the sweet almond oil also contains mineral substances and protein, and can relieve skin itching, and eliminate red swelling, dryness and inflammation.
The rosa canina fruit has the efficacies of resisting weakness, resisting fatigue and promoting digestion, and the rosa canina fruit oil contains rich vitamin C, has the effect of revivification, is rich in unsaturated fatty acid, linoleic acid, vitamin A and other components, can prevent the loss of epidermal water, moisten the skin, help the regeneration of stratum corneum, and has the effects of relieving the skin, resisting oxidation, preventing wrinkles, whitening and lightening spots and the like.
The wheat germ oil is cereal germ oil prepared by using wheat malt as a raw material, integrates the nutrition essence of wheat, is rich in vitamin E, linoleic acid, linolenic acid, glycerol octanediol and various physiological active components, and has the effects of regulating endocrine, reducing weight, and preventing color spots, black spots and pigmentation; has effects in resisting oxidation, reducing lipid peroxide generation, moistening skin, and delaying aging; promoting metabolism and skin regeneration, removing wrinkle, preventing skin aging, and eliminating scar; regulating blood lipid, softening blood vessel, and preventing arteriosclerosis, hypertension, and apoplexy.
The skin-brightening, moisturizing, soothing and anti-aging compound essential oil preferably comprises the following components in parts by weight:
3 parts of rosa damascena flower oil;
30 parts of lavender essential oil;
17 parts of jojoba seed oil;
20 parts of sweet almond oil;
20 parts of Rosa canina fruit oil;
10 parts of wheat germ oil.
The research of the inventor finds that the skin brightening, soothing, repairing, moisturizing and anti-aging effects of the compound essential oil provided by the invention are not only related to the components of the components, but also have an important relationship with the weight ratio of the components.
The compound essential oil for skin brightening, moisturizing, soothing and anti-aging is preferably prepared by the following steps: mixing fresh lavender and dry lavender according to a weight ratio of 1-3: 1, standing at-15-10 ℃ for 10-12 hours, adding water which is 3-5 times of the total weight of the lavender, adding lysozyme which is 0.1-0.2% of the total weight of the lavender, performing enzymolysis at 25-35 ℃ for 30-60 minutes, inactivating enzyme at 100-105 ℃ for 10-20 minutes to obtain a lavender aqueous solution, performing supercritical carbon dioxide extraction on the lavender aqueous solution, separating, concentrating and drying to obtain the lavender essential oil.
Further preferably, the supercritical carbon dioxide extraction conditions are: the flow rate is 25-30L/h, the pressure is 5-10 MPa, and the extraction time is 60-150 min.
According to the preparation method of the lavender essential oil, provided by the invention, the fresh and dried lavender are blended, subjected to a freezing treatment method, then subjected to enzymolysis to destroy the cell structure of the lavender, so that the content in lavender cells is effectively dissociated, and then subjected to supercritical carbon dioxide extraction, so that the yield of the lavender essential oil is improved.
The skin-brightening, moisturizing, soothing and anti-aging compound essential oil is preferably prepared by taking sweet almond, soaking, peeling, drying, crushing, Soxhlet extraction and distilling to obtain the sweet almond oil; further preferably, the extraction method comprises adding pulverized semen Pruni Armeniacae to 75% ethanol water solution, and distilling at 85-95 deg.C.
The compound essential oil for skin brightening, moisturizing, soothing and anti-aging is prepared by the following steps: taking dried rosa canina fruit, crushing, sieving, adding into water with the weight being 3-5 times of that of the dried rosa canina fruit, adding 1.5-3% of complex enzyme, adjusting the pH to 4-5, carrying out enzymolysis at 35-55 ℃ for 3-5 h, then inactivating the enzyme at 100-105 ℃ for 10-20 min, carrying out reflux extraction, separating, concentrating and drying to obtain the rosa canina fruit oil.
The Rosa canina fruit oil provided by the invention is prepared by crushing Rosa canina fruits, performing enzymolysis under acidic conditions, and then performing reflux extraction, so that the obtained Rosa canina fruit oil has high yield, and the effective components are retained.
Further preferably, the compound enzyme is cellulase and pectinase, and the weight ratio of the cellulase to the pectinase is 3-5: 1.
Further preferably, the reflux extraction is carried out at the temperature of 75-85 ℃ for 60-120 min.
The invention provides a preparation method of the skin-brightening, moisturizing, soothing and anti-aging compound essential oil, which comprises the following steps: firstly, taking lavender essential oil, then dropwise adding damascope rose flower oil into the lavender essential oil, and uniformly mixing; and then sequentially dropwise adding Rosa canina fruit oil, jojoba seed oil and sweet almond oil, uniformly mixing, adding wheat germ oil, and uniformly mixing to obtain the compound essential oil.
Firstly, taking lavender essential oil with a slow volatilization speed from main essential oil, then dropwise adding rosa damascena flower oil with a fast volatilization speed so as to slow down the volatilization speed of the essential oil, then adding base oil, adding the base oil according to the sequence of rosa canina fruit oil, jojoba seed oil and sweet almond oil, and finally adding wheat germ oil with a preservative effect; the preparation method provided by the invention can slow down the volatilization speed of the essential oil, improve the stability of the essential oil and improve the aroma level of the essential oil.
The skin-brightening, moisturizing, soothing and anti-aging composite essential oil provided by the invention can be applied to cosmetics, preferably, the amount of the composite essential oil in the cosmetics is 0.0025-20 wt%, and more preferably, the amount of the composite essential oil in the cosmetics is 0.0025-0.01 wt%, namely 25-100 mg/L.
The invention has the beneficial effects that:
1. the compound essential oil provided by the invention is prepared by compounding the damascope rosea oil, the lavender essential oil, the jojoba seed oil, the sweet almond oil, the rosa canina fruit oil and the wheat germ oil, and combining the theory of traditional Chinese medicine, the selected raw materials are sweet and flat in taste, wherein the damascope rosea oil and the lavender essential oil are mainly used, the jojoba oil, the sweet almond oil and the rosa canina fruit oil are used as base oil, and the wheat germ oil serving as a plant preservative is added, so that the compound essential oil is matched with each other and has a synergistic effect, and the good effects of black dispelling, skin brightening, allergy resistance, soothing, water replenishing and moisture keeping are achieved.
2. The compound essential oil provided by the invention contains lavender essential oil and wheat germ oil with a certain antibacterial effect, and the lavender essential oil and the wheat germ oil interact with each other, and also has the effects of improving the stability and the corrosion resistance of the compound essential oil. The compound essential oil provided by the invention is safe and reliable in component, and can be kept for 3 years without deterioration under the condition of no addition of other preservatives.
3. The compound essential oil provided by the invention takes plant essential oil as a raw material, solves the problems of low solubility, influence on product color, difficulty in absorption by skin, safety and the like in the application of the compound essential oil in cosmetics, and obviously improves the skin care effect while improving the stability of effective components. The compound essential oil provided by the invention has multiple effects of skin brightening, soothing and repairing, skin moistening, aging resistance and the like, so that the compound essential oil has a good development prospect.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
The present invention provides examples 1 to 10, wherein the compositions of the components are shown in table 1, wherein 1 part by weight is 1 g.
TABLE 1 compositions of the components of examples 1 to 10
Figure BDA0002316421790000061
Figure BDA0002316421790000071
In embodiments 1 to 10 of the present invention, the preparation method of the lavender essential oil comprises: mixing fresh lavender and dry lavender according to a weight ratio of 1-3: 1, standing at-15-10 ℃ for 10-12 hours, adding water which is 3-5 times of the total weight of the lavender, adding lysozyme which is 0.1-0.2% of the total weight of the lavender, performing enzymolysis at 25-35 ℃ for 30-60 min, deactivating enzyme at 100-105 ℃ for 10-20 min to obtain a lavender aqueous solution, and performing supercritical carbon dioxide extraction on the lavender aqueous solution, wherein the supercritical carbon dioxide extraction conditions are as follows: the flow rate is 25-30L/h, the pressure is 5-10 MPa, the extraction time is 60-150 min, and the lavender essential oil is obtained through separation, concentration and drying;
the preparation method of the sweet almond oil comprises the steps of taking sweet almonds, soaking, peeling, drying, crushing and Soxhlet extraction, wherein the extraction method comprises the steps of adding the crushed sweet almonds into 75% ethanol water solution by volume fraction, and distilling and extracting at 85-95 ℃ to obtain the sweet almond oil;
the preparation method of the rosa canina fruit oil comprises the following steps: taking dried rosa canina fruit, crushing, sieving, adding into 3-5 times of water by weight, adding 1.5-3% of complex enzyme, wherein the complex enzyme is cellulase and pectinase, the weight ratio of the cellulase to the pectinase is 3-5: 1, adjusting the pH value to 4-5, carrying out enzymolysis for 3-5 h at 35-55 ℃, inactivating the enzyme for 10-20 min at 100-105 ℃, carrying out reflux extraction under the condition that the temperature is 75-85 ℃, carrying out reflux extraction for 60-120 min, separating, concentrating and drying to obtain the rosa canina fruit oil.
The preparation method of the skin-brightening, moisturizing, soothing, and anti-aging compound essential oil of embodiments 1-10, the preparation method comprising the steps of: firstly, taking lavender essential oil, then dropwise adding damascope rose flower oil into the lavender essential oil, and uniformly mixing; and then sequentially dropwise adding Rosa canina fruit oil, jojoba seed oil and sweet almond oil, uniformly mixing, adding wheat germ oil, and uniformly mixing to obtain the compound essential oil.
Comparative example
The present invention provides comparative examples 1 to 6, the compositions of the components of comparative examples 1 to 6 are shown in Table 2, wherein 1g is given per 1 part by weight.
TABLE 2 compositions of the components of comparative examples 1 to 6
Figure BDA0002316421790000081
Test examples
1. Experimental part
1.1 materials and instruments
1.1.1 materials
The rosebush oil, the jojoba seed oil and the wheat germ oil in the plant essential oil for the test are provided by Beijing interconnected New era biotechnology limited, and other components are prepared according to the scheme provided by the invention;
mouse melanoma cells (B16), shanghai cell bank of chinese academy of sciences;
sheep red blood cell suspension with mass fraction of 2%, Guangzhou Hongquan Biotechnology Co., Ltd;
dimethyl sulfoxide (DMSO), tetramethyl azodicarbonyl blue (MTT), tyrosinase, Sigma;
l-tyrosine, sodium deoxycholate, sodium hydroxide, Sodium Dodecyl Sulfate (SDS), hydrochloric acid, absolute ethyl alcohol, chemical reagents of national drug group, Inc.;
M-RPMI-1640 medium, Fetal Bovine Serum (FBS), 100 XPcillin-streptomycin solution, trypsin-EDTA solution with a mass fraction of 0.25%, Phosphate Buffered Saline (PBS), Thermo company, USA.
1.1.2 instruments
CorneometerCM825 skin moisture content tester, TewameterTM300 moisture loss through the skin tester, MPA9-GL200 skin gloss tester, MPA9-CL400 skin color difference tester, MexametrMX 18 skin melanin and heme tester, MPA580 skin elasticity tester, Germany CK company;
VISIA-CR facial image analysis System, Canfield, USA; HL-2B digital display constant flow pump, Shanghai Jiapeng science and technology Limited;
2695 analytical high performance liquid chromatograph, Waters corporation, usa;
RE-52AA rotary evaporator, Shanghai Yanglong Biochemical apparatus factory;
a freeze dryer, Christ, germany;
LunaC18 column (250 mm. times.4.60 mm. times.5 μm), Phenomenex, USA;
eon microplate reader, BioTek corporation, usa;
SmartCell type carbon dioxide incubator, Likang biomedical science and technology Consortium GmbH;
SW-CJ-ZF clean bench, Shanghai Boxun industries, Ltd;
TDZS-WS type centrifuge, hunan instrument centrifuge instruments ltd;
LDZX-50KBS vertical autoclave, Shanghai Shenan medical instrument factory.
1.2 Experimental methods
1.2.1 in vitro experiments on skin lightening and soothing repair efficacy
The following groups used the complex essential oils of examples 1 to 10, wherein the first group used the complex essential oil obtained in example 1, the second group used the complex essential oil obtained in example 2, and so on, the comparative group used the essential oils of comparative examples 1 to 6, the first group used the essential oil obtained in comparative example 1, the second group used the essential oil obtained in comparative example 2, and so on, and the control group used β -arbutin.
1.2.1.1 culture of B16 cells
The positive control (β -arbutin), the essential oils obtained in examples 1-10 and comparative examples 1-6 were dissolved in RPMI-1640 culture medium at mass concentrations of 12.5, 25, 50 and 100mg/L, respectively, and filtered and sterilized with 0.22 μm water-phase filter membrane for use.
Using RPMI-1640 culture solution containing 10% volume fraction fetal calf serum at 37 deg.C and 5% volume fraction CO 2B16 cells were cultured under conditions. When the cells grow to be close to the fusion state, the cells are digested by trypsin with the mass fraction of 0.25 percent, and the cell density is collected and adjusted to be 5 multiplied by 10 4one/mL, 100. mu.L per well was inoculated into a 96-well plate and supplemented with 200. mu.L of culture medium at 37 ℃ with a volume fraction of 5% CO 2Culturing overnight in the environment, adding 200 μ L of test solution with different concentrations into each well after adherence, setting 6 multiple wells for each concentration, adding fresh culture solution into blank control group, and continuing culturing for 48 h.
1.2.1.2 determination of cell proliferation Rate of B16
After the test object is treated for 48 hours, 20 mu L of MTT solution with the concentration of 5mg/mL is added into each hole, the incubation is carried out in an incubator for 4 hours, the supernatant is discarded, 150 mu L of DMSO is added into each hole, the shaking is carried out for 10min, and the absorbance OD of each hole under the wavelength of 570nm is measured by a microplate reader after the uniform mixing. The cell proliferation rate was calculated according to the following formula:
cell proliferation rate (%) ═ (OD) Experiment hole-OD Blank hole)/(OD Blank control well-OD Blank hole)×100％
1.2.1.3 determination of the relative inhibition of tyrosinase Activity in B16 cells
After the test object is treated for 48 hours, the supernatant is absorbed and removed, 50 mu L of TritonX-100 aqueous solution with the mass fraction of 1 percent is added into each hole, the test object is quickly placed into a refrigerator with the temperature of 80 ℃ for freezing and storing for 30min, the test object is taken out and then melted for 20min at the temperature of 37 ℃ to completely break the cells, 10 mu L of L-DOPA solution with the mass fraction of 1 percent is added into each hole, the test object is placed into a constant temperature water bath with the temperature of 37 ℃ for reaction for 30min, and the light absorption value OD at the position of 475nm is measured by an. The relative inhibition rate of tyrosinase activity was calculated according to the following formula:
relative inhibition (%) of tyrosinase activity (1-OD) Experiment hole/OD Blank control well)×100％
1.2.1.4 determination of the amount of melanin in B16 cells
After the test object is treated for 48 hours, the supernatant is sucked away, the precipitated cells are blown into suspension by PBS buffer solution, 1mL of cell suspension is sucked into a centrifuge tube, the centrifugation is carried out for 10min at 1500r/min, then the supernatant is discarded, 200 mu LPBS buffer solution is firstly added to resuspend the cells, 1mL of ethanol-ether solution (the volume ratio is 1: 1) is added, the mixture is kept still for 20min at room temperature, the centrifugation is carried out for 5min at 3000r/min, the supernatant is discarded, 1mL of 1mol/LNaOH solution containing 10% of DMSO by mass fraction is added, the mixture is subjected to water bath at 80 ℃ for 45min, and the light absorption value OD is measured by an enzyme-labeling instrument at the wavelength of 405 nm. The intracellular melanin content was calculated according to the following formula:
intracellular relative melanin content (%) ═ OD Experiment hole/OD Blank control well×100％
1.2.1.5 human skin fibroblast proliferation Activity assay
Dissolving in dimethyl sulfoxide, filtering with 0.2mm sterile filter head for sterilization, and preparing into culture medium containing 10% sample with DMEM culture solution. Obtaining human skin fibroblast by tissue block culture method, adding 0.25% trypsin 1mL for digestion, centrifuging, adding DMEM culture solution for resuspension, counting and diluting to cell concentration of 1 × 10 5Perml, 100mL per well, seeded in 96-well plates. Culturing for 48h, observing the adherence of the cells under a mirror, and sucking away the culture medium in the holes. 5% CO at 37 ℃ 2Standing in incubator for 24h, 48h, and 72h, taking 4 holes for each group, adding 20 mM MTT solution into each hole, and adding CO 2And (3) continuously incubating for 4h in the incubator, adding 150mL of dimethyl sulfoxide into each hole, placing on a constant-temperature shaking table, oscillating at a low speed for 10min, and then measuring the light absorption value of each hole at 490nm on the microplate reader.
1.2.2 human experiments on skin protection efficacy
The following groups used the complex essential oils of examples 1 to 10, wherein the first group used the complex essential oil obtained in example 1, the second group used the complex essential oil obtained in example 2, and so on, the comparative group used the essential oils of comparative examples 1 to 6, the first group used the essential oil obtained in comparative example 1, the second group used the essential oil obtained in comparative example 2, and so on, and the control group used β -arbutin.
1.2.2.1 evaluation of skin moisturizing efficacy
51 qualified subjects (age 22-42 years) are selected to be tested, the testing temperature is (22 +/-2) DEG C, and the relative humidity is (50 +/-5)%. Before testing, 3 test areas (each 4 cm. times.4 cm) are marked on the inner side of the crank arm of the subject, the test areas are washed uniformly with clear water, and the subject is randomly divided into 17 groups with equal number, each group comprises 3 persons, and the control group is divided into 2ml/cm 2Coating β -arbutin, and making experimental group at 2ml/cm 2Applying the compound essential oil of examples 1-10 to a comparison group at a ratio of 2ml/cm 2Applying the essential oil of comparative examples 1-6. After the test subjects used the samples 1, 2 and 4 hours, the moisture content (MMV) and the transdermal moisture loss (TEWL) of the skin of the test subjects in 3 test areas were measured by a skin moisture content tester and a moisture loss tester, respectively, and the average values were obtained.
1.2.2.2 assessment of anti-aging efficacy
And selecting 48 qualified subjects (the age is 22-42 years) to take part in the test, wherein the test temperature is (22 +/-2) DEG C, and the relative humidity is (50 +/-5)%. Before the test, 1 test area was marked on each cheek and outside of the corners of both eyes of the subject (i.e., 4 test areas were marked in total, each area being 4 cm. times.4 cm), and the test areas were washed with clean water in a unified manner. The experimental or control samples were applied to one side of the subject and the control samples to the other side, eachApplying the sample once every morning and evening, wherein the application amount is 2ml/cm 2After 30 consecutive days of application and 15 and 30 days of return visit, the moisture content MMV of the skin of 4 test areas of the subject was measured, and the skin elasticity R2 and Q2 values were measured with a skin elasticity tester and averaged.
1.2.2.3 evaluation of skin lightening efficacy
The screening, grouping and experimental methods of the subjects were the same as 1.2.2.2, and the subjects were revisited at 15 d and 30d, and the melanin content (MI), the lightness (ITA degree) and the glossiness of the skin in each test area of the subjects were measured and averaged.
1.2.3 data analysis
The experimental data were analyzed for significance using the Duncan test in SPSS20 software one-way variance (ANOVA), and the results were calculated as mean ± standard deviation
Figure BDA0002316421790000121
Is represented by p<0.05 was statistically different.
2 results and discussion
2.1 in vitro experiments and results of skin lightening and soothing repair efficacy
2.1.1 Effect on B16 cell proliferation
The present invention provides the effect of each group on the proliferation of B16 cells, and the proliferation rates of B16 cells after treatment with samples of different mass concentrations are shown in Table 3.
TABLE 3 Effect of groups on B16 cell proliferation Rate (%)
Note: smaller values indicate lower cell proliferation rates, indicating better cell inhibition.
As can be seen from table 3, in 4 groups of sample masses of 12.5, 25, 50 and 100mg/L, comparison shows that the B16 cell proliferation rate of the experimental group is generally lower than that of the B16 cell proliferation rate of the comparative group and that the B16 cell proliferation rate of the comparative group is lower, which indicates that the effect of inhibiting the essential oils by combination is better than that of the single essential oils, and the compound essential oils have the effects of brightening and whitening the skin, and have the synergistic effect. Meanwhile, the comparison shows that the proliferation rate of the B16 cells in the second experimental group, namely the compound essential oil group provided in example 2, is the lowest, which indicates that the two experimental groups have a very significant effect of inhibiting the proliferation of the B16 cells (p < 0.05).
2.1.2 Effect on B16 intracellular tyrosinase Activity
The present invention provides the relative inhibition of tyrosinase activity in B16 cells by treatment of samples of different mass concentrations, the results of which are shown in table 4.
TABLE 4 relative inhibition ratio (%) -of tyrosinase activity in B16 cells by different samples
Figure BDA0002316421790000141
Note: the inhibition of tyrosinase activity gradually increased with increasing concentration
Tyrosinase is an important influencing factor in melanin synthesis, and the influence of the compound essential oil on the activity of tyrosinase is researched and provided by the invention. As can be seen from Table 4, the essential oils provided in the examples and comparative examples have inhibitory effects on the tyrosinase activity in B16 cells, and show a dose-dependent relationship, wherein the inhibitory effects on the tyrosinase activity of the compound essential oils provided in the examples are more obvious. In 4 groups of sample masses of 12.5, 25, 50 and 100mg/L, the relative inhibition rate of tyrosinase activity in B16 cells of an experimental group is generally higher than that of B16 cells of a comparison group, and the relative inhibition rate of tyrosinase activity in B16 cells of the comparison group is higher, which shows that the effect of the relative inhibition rate of the formulated essential oil on the tyrosinase activity is better than that of single essential oil, and the compound essential oil has the effects of skin brightening and whitening. The comparison shows that the relative inhibition rate of the tyrosinase activity of the second experimental group is the highest, which indicates that the inhibition effect of the tyrosinase activity in the B16 cells of the second experimental group is very obvious (p is less than 0.05).
2.1.3 Effect on the intracellular melanin content of B16
The effects of examples 1 to 10, comparative examples 1 to 6 and the positive control group on melanin production in B16 cells at different concentrations were investigated, and the results are shown in table 5.
TABLE 5 Effect of different samples on the amount of melanin in B16 cells
Figure BDA0002316421790000151
Figure BDA0002316421790000161
As can be seen from table 5, the compound essential oil can significantly inhibit the synthesis of melanin in cells, and as the concentration of the compound essential oil increases, the melanin synthesis inhibition rate significantly increases and is concentration-dependent. As can be seen from table 5, in 4 groups of sample masses of 12.5, 25, 50 and 100mg/L, the inhibition rate of melanin synthesis in B16 cells in the experimental group is generally higher than that of B16 cells in the comparative group, and the inhibition rate of melanin synthesis in B16 cells in the comparative group is higher, which indicates that the effect of the compounded essential oils on the inhibition rate of melanin synthesis is better than that of the single essential oils, and the compounded essential oils have the effects of skin lightening and whitening; the compound essential oil has a synergistic effect in whitening and brightening the skin. The comparison shows that the two pairs of the experimental groups have the highest melanin synthesis inhibition rate, which indicates that the two pairs of the experimental groups have very significant melanin synthesis inhibition effect in B16 cells (p is less than 0.05).
2.1.4 measurement of human skin fibroblast proliferation Activity
The invention researches the effect of the non-sample group on the regeneration of the human skin fibroblasts by investigating the light absorption values of the human skin fibroblasts in different time periods in the examples 1-10, the comparative examples 1-6 and the positive control group, and the result is shown in the table 6.
TABLE 6 light absorption values of human skin fibroblasts at different times for different groups
Figure BDA0002316421790000162
Figure BDA0002316421790000171
Note: the higher the absorbance, the better the effect of promoting cell regeneration and the better the effect of promoting the repair of injured tissue
From the results in table 6, it can be seen that the essential oil has the effect of promoting cell regeneration on human skin fibroblasts, promotes the repair of injured tissues, and the light absorption value is increased and is concentration-dependent with increasing time. In the time values of 24h, 48h and 72h, comparison shows that the light absorption value of the experimental group is generally higher than that of the comparison group and the light absorption value of the comparison group is higher than that of the comparison group, which shows that the essential oil after compounding has better effects of promoting regeneration and repairing of human skin fibroblasts than that of single essential oil, and the compound essential oil can play a role in synergistically repairing regeneration and repair of human skin fibroblasts. The results in table 6 show that the compound essential oil provided by the invention has a relatively obvious cell regeneration promoting effect, can promote the repair of injured tissues, and achieves the purposes of skin tendering and soothing repair. Meanwhile, the comparison shows that the light absorption value of the second experimental group is the highest compared with that of other groups, which indicates that the two pairs of human skin fibroblasts in the experimental group have very obvious effects of promoting regeneration and repair (p is less than 0.05).
In summary, the results in tables 3 to 6 all show that the composite essential oil provided in embodiments 1 to 10 of the present invention has significantly significant effects of promoting fibroblast repair, inhibiting melanin production, whitening skin, lightening spots, and soothing repair compared with the single essential oil provided in comparative examples 1 to 6 and β -arbutin provided in the positive control group, which fully demonstrates that the components in the composite essential oil provided in the present invention have synergistic effects in terms of inhibiting tyrosinase activity, inhibiting melanin production, and promoting fibroblast repair.
2.2 results of human body experiments on skin moisturizing efficacy
2.2.1 Effect on skin moisture content MMV
The influence of the positive control groups in examples 1-10 and comparative examples 1-6 on the water content of human skin within 4h is examined, and the results are shown in Table 7.
Change in skin moisture content within Table 74 h
Figure BDA0002316421790000181
Figure BDA0002316421790000191
From the results in table 7, it can be seen that, within 4 hours of the test period, the MMV value of the skin moisture content of the experimental group is generally higher than the light absorption value of the comparison group, and the MMV value of the skin moisture content of the comparison group is higher than that of the comparison group, which shows that the effect of moisturizing the skin of the compounded essential oil is better than that of the single essential oil, and the components in the compound essential oil provided by the invention play a role in synergy. The MMV value of the skin moisture content of the second experimental group is higher than that of the other groups, namely 22.67 +/-3.32 at 0h, 48.99 +/-5.14 at 1h, 42.59 +/-5.68 at 2h and 38.68+6.45 at 4h, which shows that the essential oil is not completely absorbed by the human body at the beginning of use, the moisture content of the skin is gradually increased and well maintained after the absorption of the skin, and the moisture content of the skin can still be maintained at a higher level after 4 h. The compound essential oil provided by the invention has good effects of moisturizing, wherein the effect of moisturizing the skin is very obvious (p is less than 0.05) by using an experimental group.
2.2.2 Effect on transdermal Water Dispersion (TEWL value)
The TEWL value is an index for measuring the moisture retention performance of the skin, and the smaller the value is, the less the water is lost through the skin, namely the stronger the water locking and moisture retention capacity is. The invention studies the TEWL values of the transdermal water dispersion loss in each test area group, and the results are shown in table 8.
TABLE 8 variation of the transdermal Water Dispersion loss TEWL value
From the results in table 8, it can be seen that, within 4 hours of the test period, the TEWL values of the experimental group are generally lower than those of the comparative group and the TEWL values of the comparative group are lower than those of the comparative group, which indicates that the effects of reducing skin moisture loss, moisturizing and locking water, moistening skin and moisturizing the skin of the compounded essential oil are better than those of the single essential oil, and the compound essential oil plays a role in synergy. The comparison shows that the transdermal water loss (TEWL value) of the experimental group II is the lowest compared with that of other groups, which indicates that the experimental group II has the effects of reducing the skin water loss, preserving moisture and locking water, and has very obvious effect on skin moistening (p is less than 0.05).
In summary, the results in tables 7 to 8 all show that the composite essential oil provided in examples 1 to 10 of the present invention has significantly reduced skin moisture loss and improved skin moisture content compared to the single essential oil provided in comparative examples 1 to 6 and β -arbutin provided in the positive control group, and the moisturizing effect of the composite essential oil provided in the present invention is much higher than that of the single essential oil, which indicates that the components in the composite essential oil provided in the present invention interact with each other in the moisturizing aspect to achieve the synergistic effect.
2.3 results of human body experiment for anti-aging efficacy
2.3.1 Effect on skin moisture content MMV
The influence of the positive control groups in examples 1 to 10 and comparative examples 1 to 6 on the water content of human skin within 30 days was examined, and the results are shown in Table 9.
TABLE 930 MMV changes in skin moisture content within days
Figure BDA0002316421790000221
From the results in table 9, in 30d of the test, comparison shows that the MMV value of the skin moisture content of the experimental group is generally higher than that of the comparison group, and the MMV value of the skin moisture content of the comparison group is higher than that of the comparison group, which indicates that the compounded essential oil has better effects of moisturizing the skin and delaying aging than single essential oil, and the components of the compound essential oil provided by the invention play roles of synergistically increasing moisturizing and resisting fatigue. Meanwhile, the comparison shows that the MMV value of the skin moisture content of the experimental group II is the highest compared with that of other groups, which indicates that the compound essential oil used by the experimental group II has a good moisturizing effect on human skin for a long time, and the anti-aging capability of the skin can be improved due to the increase of the skin moisture content, and this indicates that the compound essential oil provided by the invention has good moisturizing skin and anti-aging effects, wherein the moisturizing and anti-aging effects of the compound essential oil provided by the example 2 used by the experimental group II are very obvious (p is less than 0.05).
2.3.2 Effect on the R2, Q2 values of skin elasticity
The effects of examples 1-10, comparative examples 1-6, and the positive control on skin elasticity were investigated, and the results are shown in tables 10 and 11.
TABLE elastic skin R2 values over day 1030
Figure BDA0002316421790000222
Figure BDA0002316421790000231
TABLE 1130 days skin elasticity Q2 values
Figure BDA0002316421790000241
Note: skin elasticity closer values of R2 to Q2 indicate better skin elasticity.
As can be seen from the results in tables 10 and 11, in the test of 30d, through comparison, it is found that the skin elasticity R2 and Q2 values of the experimental group are generally higher than the elasticity R2 and Q2 values of the comparative group, and the elasticity R2 and Q2 values of the comparative group are higher, which indicates that the effect of improving skin elasticity of the compounded essential oil is better than that of the single essential oil, and the synergistic effect of improving skin elasticity is achieved by the mutual matching of the components in the compound essential oil. Meanwhile, the comparison shows that the skin elasticity R2 and Q2 values of the second experimental group are the highest compared with those of other groups, which indicates that the effect of improving the skin elasticity of the second experimental group is very obvious (p is less than 0.05).
In summary, the results in tables 9 to 11 all show that the composite essential oil provided in embodiments 1 to 10 of the present invention has significantly improved and maintained skin moisture and improved skin elasticity compared to the single essential oil provided in comparative examples 1 to 6 and β -arbutin provided in the positive control group, which indicates that the composite essential oil provided in the present invention has significantly improved and maintained skin moisture and improved skin elasticity compared to the single essential oil, and the components in the composite essential oil provided in the present invention can interact with each other to synergistically improve and moisturize skin elasticity, and has a good anti-aging effect.
2.4 human body test results for skin lightening efficacy
2.4.1 Effect on the MI value of the melanin content of the skin
The effects of examples 1-10, comparative examples 1-6, and the positive control on skin melanin content were tested, and the results are shown in table 12.
TABLE 1230 change in skin melanin content values within days
Figure BDA0002316421790000251
Note: the lower the MI, the better the whitening and smoothing of the skin and lightening of melanin.
From the results in table 12, it can be seen that, within 30d of the test, the skin melanin content MI value of the experimental group is generally lower than that of the comparative group, and the skin melanin content MI value of the comparative group is lower than that of the comparative group, which indicates that the effects of skin lightening and melanin lightening of the compounded essential oil are better than those of the single essential oil. Meanwhile, the comparison shows that the MI value of the skin melanin content of the experimental group II is the lowest compared with that of other groups, which indicates that the experimental group II has very obvious effects on whitening and smoothing skin and lightening melanin (p is less than 0.05).
2.4.2 Effect on skin Brightness ITA DEG value
The effects of examples 1-10, comparative examples 1-6, and the positive control on skin brightness were tested, and the results are shown in Table 13.
Changes in skin brightness values within Table 1330 days
Figure BDA0002316421790000261
Figure BDA0002316421790000271
Note: the ITA value is a value that characterizes skin brightness in relation to L and b, and the greater the ITA value, the brighter the skin, whereas the darker the skin.
As can be seen from the results of table 13, in 30d of the test, it was found by comparison that the skin brightness ITA ° value of the experimental group was generally higher than the skin brightness ITA ° value of the comparative group, and the skin brightness ITA ° value of the comparative group was higher than that of the comparative group. The compound essential oil provided by the invention has more remarkable skin brightening effect compared with single essential oil, and the interaction of the components in the compound essential oil synergistically promotes the skin brightening effect. The skin brightness ITA ° value of the second experimental group was the highest compared to the other groups, indicating that the effect of brightness enhancement was very significant in the second experimental group (p < 0.05).
2.4.3 Effect on skin gloss
The effects of examples 1-10, comparative examples 1-6, and the positive control on skin gloss were tested, and the results are shown in table 14.
TABLE 1430 days change in skin gloss
Figure BDA0002316421790000272
Figure BDA0002316421790000281
Skin glossiness is a numerical value that characterizes the shiny feel of the skin surface. As can be seen from the results in table 14, in the tested 30d, the skin gloss value of the experimental group is generally higher than that of the comparative group, and the skin gloss value of the comparative group is higher than that of the comparative group, which indicates that the composite essential oil provided by the present invention has a more significant effect in enhancing skin gloss than the single essential oil, and fully indicates that each component in the composite essential oil provided by the present invention is interactive, so as to achieve the effect of enhancing skin gloss together. The skin gloss value of the second experimental group is the highest compared with that of the other groups, which shows that the effect of the second experimental group on the glossy skin is very obvious (p is less than 0.05).
In summary, the results in tables 12 to 14 all show that the composite essential oil provided in embodiments 1 to 10 of the present invention has significant effects of reducing melanin content, increasing skin brightness, and increasing skin glossiness compared to the single essential oil provided in comparative examples 1 to 6 and β -arbutin provided in the positive control group, which indicates that the composite essential oil provided in the present invention has significant skin brightening effects compared to the single essential oil, and the components in the composite essential oil provided in the present invention can interact with each other to synergistically improve the melanin content, increase skin brightness, and increase skin glossiness, thereby having good skin brightening effects.
The invention researches the skin brightening, soothing, repairing, moisturizing and anti-aging effects of the single essential oil, the compound essential oil provided by the invention and the β -arbutin of the control group, and experimental results show that the compound essential oil provided by the invention has obvious effects of inhibiting tyrosinase activity, inhibiting formation of melanin, improving skin brightness and glossiness, promoting regeneration and repair of skin fibroblasts, rejuvenating skin, resisting aging and soothing compared with the single essential oil and β -arbutin.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (10)

1. The compound essential oil for skin brightening, moisturizing, soothing and anti-aging is characterized by comprising the following components in parts by weight:
0.1-15 parts of rosa damascena flower oil;
0.1-40 parts of lavender essential oil;
1-50 parts of jojoba seed oil;
1-50 parts of sweet almond oil;
0.1-50 parts of rosa canina fruit oil;
1-40 parts of wheat germ oil.
2. The skin-brightening, moisturizing, soothing and anti-aging compound essential oil according to claim 1, characterized by comprising the following components in parts by weight:
3 parts of rosa damascena flower oil;
30 parts of lavender essential oil;
17 parts of jojoba seed oil;
20 parts of sweet almond oil;
20 parts of Rosa canina fruit oil;
10 parts of wheat germ oil.
3. The skin-brightening, moisturizing, soothing and anti-aging composite essential oil according to claim 1 or 2, characterized in that the lavender essential oil is prepared by a method comprising: mixing fresh lavender and dry lavender according to a weight ratio of 1-3: 1, standing at-15-10 ℃ for 10-12 hours, adding water which is 3-5 times of the total weight of the lavender, adding lysozyme which is 0.1-0.2% of the total weight of the lavender, performing enzymolysis at 25-35 ℃ for 30-60 minutes, inactivating enzyme at 100-105 ℃ for 10-20 minutes to obtain a lavender aqueous solution, performing supercritical carbon dioxide extraction on the lavender aqueous solution, separating, concentrating and drying to obtain the lavender essential oil.
4. The skin-lightening, moisturizing, soothing and anti-aging compound essential oil according to claim 3, wherein the supercritical carbon dioxide extraction conditions are as follows: the flow rate is 25-30L/h, the pressure is 5-10 MPa, and the extraction time is 60-150 min.
5. The compound essential oil for skin lightening and moisturizing, soothing and anti-aging according to claim 1 or 2, wherein the Rosa canina fruit oil is prepared by the following steps: taking dried rosa canina fruit, crushing, sieving, adding into water with the weight being 3-5 times of that of the dried rosa canina fruit, adding 1.5-3% of complex enzyme, adjusting the pH to 4-5, carrying out enzymolysis at 35-55 ℃ for 3-5 h, then inactivating the enzyme at 100-105 ℃ for 10-20 min, carrying out reflux extraction, separating, concentrating and drying to obtain the rosa canina fruit oil.
6. The skin-brightening, moisturizing, soothing and anti-aging composite essential oil according to claim 5, wherein the composite enzyme is cellulase and pectinase, and the weight ratio of the cellulase to the pectinase is 3-5: 1.
7. The skin-brightening, moisturizing, soothing and anti-aging composite essential oil according to claim 5, wherein the reflux extraction is performed at a temperature of 75-85 ℃ for 60-120 min.
8. The preparation method of the skin-brightening, moisturizing, soothing and anti-aging compound essential oil according to any one of claims 1 to 7, characterized by comprising the following steps: firstly, taking lavender essential oil, then dropwise adding damascope rose flower oil into the lavender essential oil, and uniformly mixing; and then sequentially dropwise adding Rosa canina fruit oil, jojoba seed oil and sweet almond oil, uniformly mixing, adding wheat germ oil, and uniformly mixing to obtain the compound essential oil.
9. Use of the skin-lightening, moisturizing, soothing and anti-aging compound essential oil according to any one of claims 1 to 7 in cosmetics.
10. The use according to claim 9, wherein the complex essential oil is used in the cosmetic in an amount of 0.0025 to 20 wt%.
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