CN116917462A - 用于在筛选方法中使用的人脂肪肝模型细胞 - Google Patents

用于在筛选方法中使用的人脂肪肝模型细胞 Download PDF

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Publication number
CN116917462A
CN116917462A CN202280017368.1A CN202280017368A CN116917462A CN 116917462 A CN116917462 A CN 116917462A CN 202280017368 A CN202280017368 A CN 202280017368A CN 116917462 A CN116917462 A CN 116917462A
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CN
China
Prior art keywords
cells
vldl
human
medium
fatty liver
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Pending
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CN202280017368.1A
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English (en)
Chinese (zh)
Inventor
高桥真生
加国雅和
吉川奈美
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Phoenixbio Co Ltd
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Phoenixbio Co Ltd
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Publication of CN116917462A publication Critical patent/CN116917462A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202280017368.1A 2021-02-26 2022-02-24 用于在筛选方法中使用的人脂肪肝模型细胞 Pending CN116917462A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-030911 2021-02-26
JP2021030911 2021-02-26
PCT/JP2022/007494 WO2022181660A1 (ja) 2021-02-26 2022-02-24 スクリーニング方法に使用するためのヒト脂肪肝モデル細胞

Publications (1)

Publication Number Publication Date
CN116917462A true CN116917462A (zh) 2023-10-20

Family

ID=83049072

Family Applications (1)

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CN202280017368.1A Pending CN116917462A (zh) 2021-02-26 2022-02-24 用于在筛选方法中使用的人脂肪肝模型细胞

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Country Link
US (1) US20250263667A1 (https=)
EP (1) EP4299720A4 (https=)
JP (1) JP7449626B2 (https=)
CN (1) CN116917462A (https=)
CA (1) CA3211975A1 (https=)
WO (1) WO2022181660A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023058657A1 (ja) * 2021-10-04 2023-04-13 株式会社フェニックスバイオ コレステロール不含の高比重リポタンパク質粒子

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3157984B2 (ja) 1994-04-11 2001-04-23 科学技術振興事業団 初代培養肝細胞の継代培養系
CA2146735C (en) 1994-04-11 2007-02-20 Chise Tateno Liver parenchymal cells having clonal growth ability, method for obtaining same, method for subculturing same, and subculturing system of primary hepatocytes
JP3266766B2 (ja) 1994-08-23 2002-03-18 科学技術振興事業団 ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法
JPH0915225A (ja) 1995-04-28 1997-01-17 Tosoh Corp 血清リポタンパク質の分析方法
JP3014322B2 (ja) 1996-05-28 2000-02-28 科学技術振興事業団 小型肝細胞の培養方法
JP3211941B2 (ja) 1996-12-26 2001-09-25 科学技術振興事業団 ヒト小型肝細胞の取得方法と、この細胞の初代培養および継代培養方法
JP2002045087A (ja) 2000-05-19 2002-02-12 Japan Science & Technology Corp キメラ動物
JPWO2007052789A1 (ja) 2005-11-04 2009-04-30 株式会社スカイライト・バイオテック 大血管障害を判別するためのリポタンパク質の分析方法
JP2007228962A (ja) 2006-01-31 2007-09-13 Hiroshima Industrial Promotion Organization ヒト肝細胞キメララットの作製方法
WO2008001614A1 (fr) 2006-06-29 2008-01-03 Hiroshima Industrial Promotion Organization Modèle animalier de stéatohépatite non alcoolique et modèle animalier de stéatose hépatique
AU2012374750B2 (en) 2012-03-27 2017-02-02 National University Corporation Kumamoto University Humanized mouse
KR102089477B1 (ko) * 2012-08-31 2020-03-17 더 가버너스 오브 더 유니버시티 오브 알버타 1차 인간 간세포의 표현형을 갖는 세포를 생성하기 위한 방법 및 조성물
JP2014204713A (ja) 2013-03-21 2014-10-30 秋田県 肝細胞分化ステージを評価する新規測定法
WO2016210163A1 (en) * 2015-06-23 2016-12-29 Colorado State University Research Foundation Engineered model of fibrotic diseases
JP7039045B2 (ja) * 2019-08-26 2022-03-22 株式会社フェニックスバイオ ヒト脂肪肝モデル細胞
JP7379934B2 (ja) 2019-08-26 2023-11-15 マツダ株式会社 車両の後部構造

Also Published As

Publication number Publication date
JP7449626B2 (ja) 2024-03-14
US20250263667A1 (en) 2025-08-21
CA3211975A1 (en) 2022-09-01
EP4299720A4 (en) 2025-03-05
JPWO2022181660A1 (https=) 2022-09-01
EP4299720A1 (en) 2024-01-03
WO2022181660A1 (ja) 2022-09-01

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