CN116898935A

CN116898935A - Hawthorn-poria cocos zinc oral liquid and preparation method thereof

Info

Publication number: CN116898935A
Application number: CN202311051051.2A
Authority: CN
Inventors: 牟书才; 李伟; 陈永泓; 张秀春; 徐建宝
Original assignee: Heilongjiang Renhetang Biotechnology Co ltd
Current assignee: Heilongjiang Renhetang Biotechnology Co ltd
Priority date: 2023-08-21
Filing date: 2023-08-21
Publication date: 2023-10-20

Abstract

The invention provides a haw, poria cocos and zinc oral liquid and a preparation method thereof, and belongs to the technical field of medicines. The invention discloses a haw, poria cocos and zinc oral liquid which comprises the following raw materials in parts by weight: 0.5 to 5 parts of hawthorn, 0.5 to 5 parts of malt, 0.5 to 5 parts of tuckahoe, 0.5 to 5 parts of radish seed, 0.1 to 3 parts of chicken's gizzard-skin, 0.0008 to 0.002 parts of zinc gluconate, 0.1 to 5 parts of sugar, 0.1 to 5 parts of honey and 0.001 to 0.01 part of stevioside. According to the invention, the prepared haw poria cocos zinc oral liquid can effectively improve the symptoms of appetite reduction, food bias, malnutrition and the like caused by dyspepsia, improve the immunity of the organism and ensure the nutritional requirements of the organism according to the functions of strengthening spleen and stomach, soothing liver and regulating qi, promoting digestion and removing food retention, reasonable compatibility and scientific proportion.

Description

Hawthorn-poria cocos zinc oral liquid and preparation method thereof

Technical Field

The invention relates to the technical field of medicines, in particular to a hawthorn, poria cocos and zinc oral liquid and a preparation method thereof.

Background

Dyspepsia (Dysppsin) is a clinical syndrome that is a disease caused by gastric motility disorders, and also includes gastroparesis with poor gastric motility and esophageal reflux disease. In dyspepsia, food is accumulated in intestines and stomach, and the intestinal vermicular ability is poor, so that discomfort symptoms such as abdominal pain, vomiting and diarrhea can appear, and the health is seriously affected. Chronic gastroenteropathy, such as antral gastritis, may be caused by long-term dyspepsia, and inappetence and hypoimmunity may be caused by untimely treatment to increase difficulty in treatment, so that malnutrition and immunity may be gradually caused, and various diseases may occur. There are many current drugs and methods for treating dyspepsia, such as omeprazole, pantoprazole, morpholine, metoclopramide, etc., which have quick and good effects, but have great side effects and are easy to cause gastrointestinal irritation. The traditional Chinese medicine has little toxic and side effect, but usually has the problems of insufficient curative effect, unobvious active ingredients and the like.

Disclosure of Invention

The invention aims to provide a haw, poria cocos and zinc oral liquid and a preparation method thereof. According to the invention, the prepared haw poria cocos zinc oral liquid can effectively improve the symptoms of appetite reduction, food bias, malnutrition and the like caused by dyspepsia, improve the immunity of the organism and ensure the nutritional requirements of the organism according to the functions of strengthening spleen and stomach, soothing liver and regulating qi, promoting digestion and removing food retention, reasonable compatibility and scientific proportion.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a haw, poria cocos and zinc oral liquid which is prepared from the following raw materials in parts by weight: 0.5 to 5 parts of hawthorn, 0.5 to 5 parts of malt, 0.5 to 5 parts of tuckahoe, 0.5 to 5 parts of radish seed, 0.1 to 3 parts of chicken's gizzard-skin, 0.0008 to 0.002 parts of zinc gluconate, 0.1 to 5 parts of sugar, 0.1 to 5 parts of honey and 0.001 to 0.01 part of stevioside.

The invention also provides a preparation method of the haw, poria cocos and zinc oral liquid, which comprises the following steps:

(1) Decocting fructus crataegi, fructus Hordei Germinatus, raphani semen, poria, and endothelium corneum Gigeriae Galli in water, and concentrating to obtain concentrated solution;

(2) Dissolving zinc gluconate, stevioside and honey in water to obtain a solvent A;

(3) Dissolving sugar in water to obtain a solvent B;

(4) Mixing the concentrated solution, the solvent A and the solvent B, and carrying out solid-liquid separation, fluid infusion, canning and sterilization to obtain the hawthorn tuckahoe zinc oral liquid.

Preferably, the water in the step (1) is added in an amount which is 6 to 10 times of the total mass of the hawthorn, the malt, the radish seed, the tuckahoe and the chicken's gizzard-membrane.

Preferably, the times of the decoction in the step (1) are 2-3 times, and the time of each decoction is 1-3 hours.

Preferably, the density of the concentrated solution is 1.06-1.08.

Preferably, the mass ratio of the water to the honey in the step (2) is (1.5-3): 1.

preferably, the mass ratio of water to sugar in the step (3) is (0.5-2): 1.

preferably, the temperature of the solvent B is 80-100 ℃.

Preferably, the volume-mass ratio of the solution after the liquid supplementing to the hawthorn is 10L: (0.5-5) kg.

The invention provides a haw, poria cocos and zinc oral liquid and a preparation method thereof. The invention is based on the functions of strengthening the spleen and harmonizing the stomach, soothing the liver and regulating qi, promoting digestion and removing food stagnation, takes hawthorn, malt, radish seed, poria cocos and chicken gizzard-membrane as main raw materials, wherein the hawthorn is rich in rich nutritional ingredients such as protein, fat, carbohydrate, vitamins, mineral substances and organic acid, and is used for meat food stagnation and stomach stagnationDistention and fullness in the stomach, diarrhea, abdominal pain, diarrhea and dislike; malt is rich in zinc, antioxidants, B vitamins and other important vitamins and minerals, and can be used for treating dyspepsia, abdominal distention and pain, spleen deficiency and anorexia; radish seed is rich in vitamins and minerals, including vitamin A and vitamin B ₁ Vitamin B ₂ Vitamin C, vitamin E, calcium, ferrum, zinc, potassium, etc. for reducing qi and phlegm, resolving food stagnation, treating food stagnation and qi stagnation, diarrhea, cough with excessive phlegm, abdominal distention, diarrhea, and dyspnea; poria is rich in proteins and pachyman, pachymic acid, fatty acid, lecithin, adenine, protease, triterpenes, etc., and can be used for promoting diuresis, eliminating dampness, invigorating spleen, calming heart, relieving anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia. The chicken's gizzard-membrane is rich in gastric hormone, keratin, amino acid, amylase and other components, and can greatly stimulate gastric secretion and promote gastrointestinal motility. The invention adopts a scientific method to extract the effective components in hawthorn, malt, radish seed, tuckahoe and chicken's gizzard-membrane, and on the basis, zinc gluconate is added, so that the absorption and utilization of the effective components by the organism are further promoted, and the aims of effectively improving the symptoms of appetite reduction, food preference, malnutrition and the like caused by dyspepsia, improving the immunity of the organism, ensuring the nutrition requirement of the organism and promoting the growth and development of children are fulfilled.

Detailed Description

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Example 1

The embodiment provides a haw, poria cocos and zinc oral liquid, which is prepared by the following steps:

weighing 3kg of hawthorn, 3kg of malt, 2kg of poria cocos, 2kg of radish seeds and 2kg of chicken's gizzard-membrane, mixing the above medicinal materials, adding 120kg of water, decocting for 2 hours, and filtering to obtain a first decoction; adding 120kg of water, decocting for 2h, and filtering to obtain a second decoction. Combining the two decoctions, concentrating under reduced pressure to density of 1.08 (measured at 70deg.C) to obtain concentrated solution.

0.001kg of zinc gluconate, 3kg of honey, 0.01kg of stevioside and 4.5kg of water are mixed and stirred until all components are completely dissolved, so as to obtain a solvent A.

Mixing 0.5kg of white granulated sugar with 1kg of water, heating to 90 ℃, and uniformly stirring to obtain a solvent B.

Mixing the concentrated solution, the solvent A and the solvent B, filtering, supplementing purified water to volume of 10L, subpackaging into 1000 pieces, sterilizing 10ml each in a steam sterilizing cabinet at 115 ℃ for 30min to obtain the hawthorn and poria cocos zinc oral liquid.

Example 2

weighing 5kg of hawthorn, 5kg of malt, 0.5kg of poria cocos, 0.5kg of radish seed and 0.2kg of chicken's gizzard-membrane, mixing the above medicinal materials, adding 62kg of water, decocting for 2 hours, and filtering to obtain a first decoction; adding 62kg of water, decocting for 2h, and filtering to obtain a second decoction. Combining the two decoctions, concentrating under reduced pressure to density of 1.06 (measured at 70deg.C) to obtain concentrated solution.

0.002kg of zinc gluconate, 0.5kg of honey, 0.001kg of stevioside and 0.3kg of water are mixed and stirred until the components are completely dissolved, thus obtaining a solvent A.

1kg of white granulated sugar and 1kg of water are mixed, heated to 80 ℃ and stirred uniformly to obtain a solvent B.

Example 3

weighing 0.5kg of hawthorn, 0.5kg of malt, 3kg of poria cocos, 5kg of radish seeds and 3kg of chicken's gizzard-membrane, mixing the above medicinal materials, adding 50kg of water, decocting for 2 hours, and filtering to obtain a first decoction; adding 50kg of water, decocting for 2h, and filtering to obtain a second decoction. Combining the two decoctions, concentrating under reduced pressure to density of 1.07 (measured at 70deg.C) to obtain concentrated solution.

0.0008kg of zinc gluconate, 0.5kg of honey, 0.003kg of stevioside and 7.5kg of water are mixed and stirred until the components are completely dissolved, thus obtaining a solvent A.

Mixing 0.5kg of white granulated sugar with 0.5kg of water, heating to 100 ℃, and uniformly stirring to obtain a solvent B.

Comparative example 1

This comparative example differs from example 1 only in that yam and orange peel were also added, and the other operations were the same as example 1.

Comparative example 2

The comparative example is different from example 1 only in that coix seed, lotus seed, jujube and medicated leaven are also added, and other operations are the same as example 1.

Comparative example 3

This comparative example differs from example 1 only in that malt was omitted, and the other operations were the same as in example 1.

Comparative example 4

This comparative example differs from example 1 only in that radish seed was omitted, and the other operations were the same as in example 1.

Comparative example 5

The comparative example differs from example 1 only in that the endothelium corneum Gigeriae Galli was omitted, and the other operations were the same as in example 1.

Comparative example 6

This comparative example differs from example 1 only in that zinc gluconate was omitted and the other operations were the same as in example 1.

Test example 1

The test example carries out an accelerated stability test on the oral liquids of examples 1 to 3 and comparative examples 1 to 6, 10 oral liquids are taken from each group, the oral liquids are placed for 3 months at 37 ℃ and 75% humidity, 1 oral liquid is taken for detection at the time of 0 th month, 1 oral liquid is taken for 3 months, and the detection indexes and the detection results are shown in tables 1 to 5.

Table 1 stability test index and test results of oral liquid of each group

Table 2 stability test index and test results for each group of oral liquids

Table 3 stability test index and test results for each group of oral liquids

Table 4 stability test index and test results for each group of oral liquids

Table 5 stability test index and test results for each group of oral liquids

As can be seen from tables 1 to 5, each group of oral liquid is accelerated for 3 months at 38 ℃ and 75% humidity, and each index has no obvious change.

Test example 2

The test example adopts animal experiments, verifies the digestion promoting function of each group of oral liquid, and the specific process is as follows:

1. SPF-grade SD male rats (weight 120-140 g, available from Tianler Biotechnology Co., ltd., long sand) were selected 100 and equally divided into 10 groups of 10, respectively, control group, example 1 group, example 2 group, example 3 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group, comparative example 5 group, comparative example 6 group. Normal feeding was performed using normal feed (purchased from tasha taylor biotechnology limited). Wherein, the control group is filled with distilled water in a dosage of stomach (1 ml/100g body weight) every day, and the other groups are filled with oral liquid in corresponding groups in a dosage of stomach (1 ml/100g body weight) once a day, and the experiment is continued for 21 days. Average body weights of rats were recorded weekly and statistical results of body weight changes are shown in table 6.

Table 6 weight change (g) of mice in each group

Group of	Initial body weight	First week of	Second week	Third week	Weight change amount
						Control group	135.6	182.5	219.6	266.5	130.9
Example 1 group	137.2	181.9	223.8	271.7	134.5
						Example 2 group	129.6	185.0	221.6	266.1	136.5
Example 3 group	139.2	183.9	220.7	265.3	126.1
						Comparative example 1 group	145.3	178.2	219.3	284.9	139.6
Comparative example 2 group	138.0	180.4	222.2	270.3	132.3
						Comparative example 3 group	137.1	179.4	230.1	263.9	126.8
Comparative example 4 group	142.7	179.0	218.7	271.2	128.5
						Comparative example 5 group	138.5	183.2	225.4	277.8	139.3
Comparative example 6 group	137.6	178.5	231.0	268.7	131.1

As can be seen from table 6, the effect of each group of oral liquid on the weight and weight gain of rats was not significantly different from that of the control group. The results show that the oral liquids in each group can not affect the normal organism.

After the experiment, the rats of each group were fasted for 24 hours, the rats were anesthetized with diethyl ether, gastric juice discharged within 4 hours was collected by pylorus ligation, 1ml of gastric juice was taken and placed in a 50ml Erlenmeyer flask, 15ml of 0.05mol/L hydrochloric acid solution was added and shaken well, and two freshly prepared protein tubes were placed. The bottle mouth was closed, incubated in a 37℃incubator for 24 hours, the protein tube was taken out, the length (mm) of the transparent portions at both ends of the protein tube was measured with a ruler, the average value was calculated from the four-terminal values, and pepsin activity and pepsin discharge amount were calculated as follows:

pepsin activity (μ/ml) =four-end protein tube clear section length average ² *16

Pepsin output (μ/h) =pepsin activity amount of gastric fluid per hour

The results of pepsin activity and pepsin output calculations for each group of mice are shown in table 7.

TABLE 7 calculation of pepsin Activity and pepsin output of mice of each group

As can be seen from table 7, the remaining oral liquids of each group were able to increase gastric juice discharge, pepsin activity and pepsin discharge of rats compared to the control group, wherein the oral liquids of examples 1 to 3 had the most remarkable promotion effects on gastric juice discharge, pepsin activity increase and pepsin discharge of rats. Among the groups of comparative examples, the rat gastric juice discharge, the pepsin activity increase and the pepsin discharge amount of the group 6 of comparative example were minimized, which showed that the effect of promoting the rat gastric juice discharge, the pepsin activity increase and the pepsin discharge was not obvious only by using the Chinese medicinal components to prepare the oral liquid.

2. Male mice of Kunming species (weight 18-22 g, available from Tianler Biotechnology Co., ltd. In Changsha) were selected 110 and equally divided into 10 groups of 10, respectively, control group, model group, example 1 group, example 2 group, example 3 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group, comparative example 5 group, comparative example 6 group, each group being 10. Wherein, the control group and the model group are filled with normal saline in the dosage of stomach (0.2 ml/10g body weight) every day, and the other groups are filled with oral liquid in the corresponding group in the dosage of stomach (0.2 ml/10g body weight) once a day, and the experiment is continued for 21 days. Animals of each group were fasted for 16 hours before the end of the test, and on the day of the measurement, the control group and the model group were subjected to one-time gavage of distilled water, and after 30 minutes, each group was given 0.025% of compound diphenoxylate (dosage of 0.2ml/10g body weight) except the control group, and the control group was given an equal amount of distilled water. After 30 minutes, each group of ink is given again, after 25 minutes, the cervical vertebrae are removed to kill animals, the abdominal cavity is opened to separate the mesentery, the intestinal canal from the pylorus and the lower end to the ileocecum is cut off, the intestinal canal is placed on a tray, the small intestine is gently pulled into a straight line, the length of the intestinal canal is measured to be the total length of the small intestine, the front edge from the pylorus to the ink is the pushing length of the ink, the pushing rate of the ink is calculated, and the calculation formula is as follows:

ink advance (%) = ink advance length (cm)/small intestine full length x 100%

The results of the ink thrust rate calculations for each group of mice are shown in table 8.

Table 8 results of the ink Propulsion rate calculations for groups of mice

As can be seen from table 8, compared with the control group, the ink propulsion rate of the mice in the model group is obviously reduced, the ink propulsion rates of the mice in the examples 1 to 3 and the mice in the comparative examples 1 to 6 are obviously higher than that in the model group, and the ink propulsion rates of the mice in the examples 1 to 3 are higher than those in the mice in the comparative examples 1 to 6, so that the oral liquid of the invention can promote the movement of small intestine. In each group of the comparative examples, the mice in the group 6 have the smallest ink propelling rate, which shows that the oral liquid prepared by the traditional Chinese medicine components has no obvious effect of promoting the small intestine movement of the mice.

Test example 3

Clinical experiments are adopted in the test example, and the digestion promoting functions of the oral liquids of the examples 1 to 3 and the comparative examples 1 to 6 are verified, wherein the specific processes are as follows:

subject inclusion criteria:

the weight of the children 7-10 years old, which is caused by the poor diet alone, is within the average normal weight value of less than 1 standard deviation of the same age, and is accompanied by dyspepsia such as anorexia, food consumption reduction, food preference and the like.

Subject exclusion criteria:

acute and chronic diarrhea; the feces are routinely checked for worm egg positives; systemic disease patients who combine cardiovascular, hepatic, renal and hematopoietic systems; taking items related to the function under test for a short period of time affects the outcome judgement.

The test method comprises the following steps:

subjects were randomly divided into 4 groups, control, example 1, example 2, example 3, comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6, 20 persons each. The control group took placebo daily, and the other groups took oral liquid of the corresponding group 3 times daily, 1 each time, for 7 days continuously.

Detecting the index:

(1) Appetite:

the improvement of appetite is mainly described as good appetite (3 points), poor appetite (2 points) and poor appetite (1 point). After the oral liquid is taken, the appetite is changed from poor appetite to good appetite, and the poor appetite is changed into appetite or the appetite can be changed into good appetite; the appetite was not changed to be ineffective, and the effective rate was calculated. Appetite scores were calculated and statistically analyzed for all children tested. The statistical results are shown in Table 9.

(2) Feed intake:

the total feeding amount (including staple food, subsidiary food, vegetables and fruits) of each tested child 3 days before and after taking the medicine was recorded, and the average feeding amount for 1 day was calculated by continuous 3 days observation. The calculation results are shown in Table 10.

(3) Food preference:

the evaluation of food preference is divided into three stages, namely no food preference (no food preference at meal, 3 min), medium food preference (food preference at meal but food intake under persuasion, 2 min), and food preference (severe food preference at meal, no food intake under persuasion, 1 min). After the oral liquid is taken, the food preference evaluation is effective in that the food preference is changed into medium food preference, the food preference is changed into no food preference or the medium food preference is changed into no food preference; the evaluation of the food preference is not changed into invalid, and the effective rate is calculated. All children tested calculated the food preference score and were statistically analyzed. The statistical results are shown in Table 11.

(4) Body weight measurement and hemoglobin content measurement:

all children tested were measured for their body weight and hemoglobin content before and after dosing. The measurement results are shown in Table 12.

Table 9 results of appetite statistics for groups of children tested

As can be seen from table 9, the oral liquids of examples 1 to 3 and comparative examples 1 to 6 each significantly improved appetite of the test children compared to the control group, wherein the oral liquids of examples 1 to 3 had the most significant effect of improving appetite of the test children.

Table 10 statistical results of the variation of the intake of tested children

As can be seen from Table 10, the oral liquids of examples 1 to 3 and comparative examples 1 to 6 each significantly improved the feeding rate of the tested children compared with the control group, wherein the oral liquids of examples 1 to 3 had the most significant effect of improving the feeding rate of the tested children.

Table 11 statistics of the evaluation of the food preference of the tested children

As can be seen from Table 11, the oral liquids of examples 1 to 3 and comparative examples 1 to 6 each significantly improved the feeding bias of the children tested, compared with the control group, wherein the oral liquids of examples 1 to 3 had the most significant effect of improving the feeding bias of the children tested.

Table 12 changes in weight and hemoglobin content of groups of children

As can be seen from Table 12, the oral liquids of examples 1 to 3 and comparative examples 1 to 6 each significantly improved the weight of children compared to the control group, wherein the oral liquids of examples 1 to 3 showed the most significant improvement effect on the weight of the children. After taking each group of oral liquid, the hemoglobin content of the tested children is increased, but the change is not obvious, which indicates that the oral liquid can improve the weight of the children, but can not cause damage to the health of the children.

The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims

1. The haw and poria cocos zinc oral liquid is characterized by being prepared from the following raw materials in parts by weight: 0.5 to 5 parts of hawthorn, 0.5 to 5 parts of malt, 0.5 to 5 parts of tuckahoe, 0.5 to 5 parts of radish seed, 0.1 to 3 parts of chicken's gizzard-skin, 0.0008 to 0.002 parts of zinc gluconate, 0.1 to 5 parts of sugar, 0.1 to 5 parts of honey and 0.001 to 0.01 part of stevioside.

2. A method for preparing the hawthorn tuckahoe zinc oral liquid according to claim 1, which is characterized by comprising the following steps:

(3) Dissolving sugar in water to obtain a solvent B;

3. The preparation method according to claim 2, wherein the water in the step (1) is added in an amount of 6-10 times the total mass of the hawthorn, the malt, the radish seed, the tuckahoe and the chicken's gizzard-membrane.

4. The method according to claim 3, wherein the number of times of the decoction in the step (1) is 2 to 3, and the time of each decoction is 1 to 3 hours.

5. The method according to claim 4, wherein the density of the concentrated solution is 1.06 to 1.08.

6. The method according to claim 5, wherein the mass ratio of water to honey in step (2) is (1.5-3): 1.

7. the method according to claim 6, wherein the mass ratio of water to sugar in step (3) is (0.5 to 2): 1.

8. the process according to claim 7, wherein the temperature of the solvent B is 80 to 100 ℃.

9. The preparation method according to claim 8, wherein the volume-mass ratio of the solution after the fluid infusion to the hawthorn is 10L: (0.5-5) kg.