CN109316598B - Traditional Chinese medicine, antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating pig diarrhea as well as preparation method and application - Google Patents
Traditional Chinese medicine, antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating pig diarrhea as well as preparation method and application Download PDFInfo
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- CN109316598B CN109316598B CN201811226899.3A CN201811226899A CN109316598B CN 109316598 B CN109316598 B CN 109316598B CN 201811226899 A CN201811226899 A CN 201811226899A CN 109316598 B CN109316598 B CN 109316598B
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Abstract
The invention provides a traditional Chinese medicine for preventing and treating pig diarrhea, an antibacterial peptide and Chinese herbal medicine composite preparation and a preparation method and application thereof, and the traditional Chinese medicine for preventing and treating pig diarrhea comprises the following raw materials, by weight, 50-60 parts of codonopsis pilosula, 50-60 parts of poria cocos, 50-60 parts of bran-fried bighead atractylodes rhizome, 50-60 parts of Chinese yam, 50-60 parts of fried white hyacinth bean, 10-20 parts of fructus evodiae, 10-20 parts of stewed myrobalan, 10-20 parts of rhizoma alismatis, 10-15 parts of halloysite, 25-35 parts of lotus seed, 25-35 parts of bran-fried semen coicis, 15-25 parts of semen euryales, 10-20 parts of fructus amomi, 25-35 parts of platycodon grandiflorum, 25-35 parts of schisandra chinensis, 25-35 parts of pericarpium citri reticulatae, 25-35 parts of netmeg, 25-35 parts of scorched hawthorn fruit, 25-35 parts of scorched medicated leaven, 25-35 parts of scorched malt, 10-20 parts of endothelium corneum gigeriae galli, 15-25 parts of galangal and 25-35 parts of liquorice. Good prevention and treatment effect, no toxic or side effect and no drug residue.
Description
Technical Field
The invention relates to a traditional Chinese veterinary medicine, in particular to a traditional Chinese medicine, antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating porcine diarrhea, a preparation method and application thereof.
Background
Nowadays, the livestock breeding industry has developed to a new height, the development of the modern breeding industry is a great trend of intensive, large-scale, high-quality, green and residue-free, the use amount of antibiotics is firstly controlled to ensure that animal products are high-quality and have no drug residues, and even the application of the antibiotics is completely eradicated, which is a big concern about the health of contemporary people and offspring. However, since the antibiotics were produced in large scale in the 40 th 19 th century, especially because of the increased risk of epidemic diseases caused by the enlargement of the scale of feeding, the use of antibiotics for preventing and treating livestock and poultry diseases has become a legal material in the habitual thinking and practical production of people, the drug resistance rate of animal-derived bacteria is increased, the treatment effect of the veterinary antibacterial drugs is reduced, the dosage in the breeding link is increased, the toxic and side effects and the residual standard-exceeding risk of the veterinary antibacterial drugs are increased, the quality safety and the public health safety of livestock products are seriously threatened, and hidden troubles are brought to the health of human beings and animals. Therefore, in order to ensure the safety of animal derived foods, development of new technologies and new products for replacing animal husbandry antibiotics is urgently needed.
In addition, because of the drug residues of the antibiotics, people pursue health and health care, and certain conflict emotion is presented to livestock and poultry meat products which excessively use the antibiotics, so that the sale of the livestock and poultry meat products is influenced.
Disclosure of Invention
In order to solve the problems, the invention provides a traditional Chinese medicine, antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating porcine diarrhea, a preparation method and application thereof, and the preparation has good prevention and treatment effect, no toxic or side effect and no medicine residue.
The object of the invention is achieved in the following way: a traditional Chinese medicine for preventing and treating pig diarrhea comprises, by weight, 50-60 parts of codonopsis pilosula, 50-60 parts of poria cocos, 50-60 parts of bran-fried bighead atractylodes rhizome, 50-60 parts of Chinese yam, 50-60 parts of fried white hyacinth bean, 10-20 parts of fructus evodiae, 10-20 parts of stewed myrobalan, 10-20 parts of rhizoma alismatis, 10-15 parts of red halloysite, 25-35 parts of lotus seed, 25-35 parts of bran-fried semen coicis, 15-25 parts of semen euryales, 10-20 parts of fructus amomi, 25-35 parts of platycodon grandiflorum, 25-35 parts of schisandra chinensis, 25-35 parts of pericarpium citri reticulatae, 25-35 parts of nutmeg, 25-35 parts of scorched hawthorn fruit, 25-35 parts of scorched medicated leaven, 25-35 parts of scorched malt, 10-20 parts of chicken, 15-25 parts of rhizoma zingiberis and 25-35 parts of liquorice.
60 parts of codonopsis pilosula, 60 parts of poria cocos, 60 parts of bran-fried bighead atractylodes rhizome, 60 parts of Chinese yam, 60 parts of fried white hyacinth bean, 15 parts of fructus evodiae, 15 parts of roasted myrobalan, 15 parts of rhizoma alismatis, 10 parts of red halloysite, 30 parts of lotus seed, 30 parts of bran-fried semen coicis, 20 parts of semen euryales, 15 parts of fructus amomi, 30 parts of platycodon grandiflorum, 30 parts of schisandra chinensis, 30 parts of pericarpium citri reticulatae, 30 parts of netmeg, 30 parts of scorched hawthorn fruit, 30 parts of scorched medicated leaven, 30 parts of scorched malt, 15 parts of endothelium corneum gigeriae galli, 20 parts of galangal and 30 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving by a 100-120-mesh sieve after crushing, enabling the amount of the medicine powder passing through the sieve to be more than 70%, and collecting the sieved medicine powder for later use; (3) putting the powder which is not sieved into water which is 8-12 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 30-45 minutes, and merging and standing the extracting solutions of the two times; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; after suction filtration, carrying out reduced pressure concentration to 0.03-0.06 MPa, keeping the temperature at 70-80 ℃, and concentrating to obtain an extract with the relative density of 1.20 at 60-70 ℃; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is (1) - (15-20).
The weight ratio of the enterobactin peptide to the traditional Chinese medicine is 1: 15.
The application method of the antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating the pig diarrhea comprises the step of adding 0.3-0.6% of the weight of the pig feed into the pig feed.
The pig feed is added with 0.5 percent of antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea by weight.
The invention has the beneficial technical effects that: the traditional Chinese medicine for preventing and treating pig diarrhea provided by the invention takes codonopsis pilosula and bighead atractylodes rhizome for tonifying qi and spleen as main medicines, takes tuckahoe, white hyacinth bean, coix seed and Chinese yam for treating bland taste and dampness, lotus seed for treating astringent taste, evodia rutaecarpa for warming spleen and tonifying kidney, and supporting yang to arrest diarrhea, gordon euryale seed for tonifying kidney and spleen, removing dampness and stopping diarrhea, schisandra chinensis and myrobalan for treating diarrhea with astringents, alisma for treating diarrhea and dysentery, red halloysite for treating intestine astringency and hemostasis, and dried orange peel for regulating qi, so that the main medicines are assisted for tonifying spleen, and the main medicines for eliminating dampness and stopping diarrhea are taken as auxiliary medicines; fructus amomi is used as an adjuvant drug for activating spleen and assisting transportation and transformation, activating qi movement up and down, stopping vomiting and diarrhea, the nutmeg is the kernel of nutmeg, stimulating appetite and helping digestion, the scorched hawthorn fruit, the scorched medicated leaven, the scorched malt and the chicken's gizzard-membrane help digestion and promote digestion, and the galangal is used for warming stomach and dispelling cold; the root of balloonflower introduces herbs into lung to tonify lung qi, and the root of licorice root, radix Glycyrrhizae coordinates the effects of the other drugs in the recipe. The medicines are combined to play the roles of benefiting spleen and stomach, removing food retention, resolving dampness, harmonizing stomach and stopping diarrhea, and the traditional Chinese medicine has no toxic or side effect, no residue and no increase of drug resistance of germs.
The invention combines the antibacterial peptide of modern biotechnology product with the traditional Chinese herbal medicines for veterinarians to replenish qi and invigorate the spleen, eliminate dampness, eliminate food retention and check diarrhea to prepare the antibacterial peptide and Chinese herbal medicine compound preparation for use, can effectively kill bacteria and viruses which cause the pig diarrhea, invigorate the spleen and replenish qi, eliminate dampness and check diarrhea, gradually recovers the functions of intestines and stomach, can enhance the immune function of organisms, improve the anti-stress and anti-disease capability, improve the production performance and improve the growth speed, is particularly suitable for the case with unobvious antibiotic treatment effect, can fully embody the advantages of the antibacterial peptide and the traditional Chinese medicine, leads the drug effect and the function to more comprehensively achieve the aim of treating both principal and secondary aspect of disease, is not easy to generate drug resistance, has no toxic and harmful substance residue, accords with the anti-reducing and anti-substituting policies advocated in recent years, can reduce the problems of antibiotic use, drug residue, abuse and the like, improves the pork quality, and meets the pure natural requirement of green food, is beneficial to the group prevention and control of animal epidemic diseases.
Detailed Description
Example 1:
a traditional Chinese medicine for preventing and treating pig diarrhea comprises, by weight, 50-60 parts of codonopsis pilosula, 50-60 parts of poria cocos, 50-60 parts of bran-fried bighead atractylodes rhizome, 50-60 parts of Chinese yam, 50-60 parts of fried white hyacinth bean, 10-20 parts of fructus evodiae, 10-20 parts of stewed myrobalan, 10-20 parts of rhizoma alismatis, 10-15 parts of red halloysite, 25-35 parts of lotus seed, 25-35 parts of bran-fried semen coicis, 15-25 parts of semen euryales, 10-20 parts of fructus amomi, 25-35 parts of platycodon grandiflorum, 25-35 parts of schisandra chinensis, 25-35 parts of pericarpium citri reticulatae, 25-35 parts of nutmeg, 25-35 parts of scorched hawthorn fruit, 25-35 parts of scorched medicated leaven, 25-35 parts of scorched malt, 10-20 parts of chicken, 15-25 parts of rhizoma zingiberis and 25-35 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving by a 100-120-mesh sieve after crushing, enabling the amount of the medicine powder passing through the sieve to be more than 70%, and collecting the sieved medicine powder for later use; (3) putting the powder which is not sieved into water which is 8-12 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 30-45 minutes, and merging and standing the extracting solutions of the two times; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; after suction filtration, carrying out reduced pressure concentration to 0.03-0.06 MPa, keeping the temperature at 70-80 ℃, and concentrating to obtain an extract with the relative density of 1.20 at 60-70 ℃; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is (1) - (15-20).
The application method of the antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating the pig diarrhea comprises the step of adding 0.3-0.6% of the weight of the pig feed into the pig feed.
Example 2:
a traditional Chinese medicine for preventing and treating pig diarrhea comprises the following raw materials, by weight, 60 parts of codonopsis pilosula, 60 parts of poria cocos, 60 parts of bran-fried bighead atractylodes rhizome, 60 parts of Chinese yam, 60 parts of fried white hyacinth bean, 15 parts of fructus evodiae, 15 parts of stewed myrobalan, 15 parts of rhizoma alismatis, 10 parts of red halloysite, 30 parts of lotus seed, 30 parts of bran-fried semen coicis, 20 parts of gordon euryale seed, 15 parts of fructus amomi, 30 parts of platycodon grandiflorum, 30 parts of schisandra chinensis, 30 parts of dried orange peel, 30 parts of nutmeg, 30 parts of scorched hawthorn fruit, 30 parts of scorched medicated leaven, 30 parts of scorched malt, 15 parts of endothelium corneum gigeriae galli, 20 parts of galangal and 30 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 100-mesh sieve after crushing, wherein the amount of the sieved medicinal powder reaches 70%, and collecting the sieved medicinal powder for later use; (3) putting the powder which is not sieved into water which is 10 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 45 minutes, combining the two extracting solutions and standing; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum-filtering, concentrating under reduced pressure to 0.03MPa, maintaining the temperature at 80 deg.C, and concentrating to obtain extract with relative density of 1.20 at 60 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 15.
Example 3
A traditional Chinese medicine for preventing and treating pig diarrhea comprises the following raw materials, by weight, 50 parts of codonopsis pilosula, 50 parts of poria cocos, 50 parts of bran-fried bighead atractylodes rhizome, 50 parts of Chinese yam, 50 parts of fried white hyacinth bean, 10 parts of fructus evodiae, 10 parts of stewed myrobalan, 10 parts of rhizoma alismatis, 10 parts of red halloysite, 25 parts of lotus seed, 25 parts of bran-fried semen coicis, 15 parts of gordon euryale seed, 10 parts of fructus amomi, 25 parts of platycodon grandiflorum, 25 parts of schisandra chinensis, 25 parts of dried orange peel, 25 parts of nutmeg, 25 parts of scorched hawthorn fruit, 25 parts of scorched medicated leaven, 25 parts of scorched malt, 10 parts of endothelium corneum gigeriae galli, 15 parts of galangal and 25 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 120-mesh sieve after crushing, wherein the amount of the sieved medicinal powder reaches 82%, and collecting the sieved medicinal powder for later use; (3) putting the powder which is not sieved into water which is 8 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 30 minutes, combining the two extracting solutions and standing; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum-filtering, concentrating under reduced pressure to 0.06MPa, maintaining the temperature at 70 deg.C, and concentrating to obtain extract with relative density of 1.20 at 70 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 16.
Example 4
A traditional Chinese medicine for preventing and treating pig diarrhea comprises the following raw materials, by weight, 60 parts of codonopsis pilosula, 60 parts of poria cocos, 60 parts of bran-fried bighead atractylodes rhizome, 60 parts of Chinese yam, 60 parts of fried white hyacinth bean, 20 parts of fructus evodiae, 20 parts of stewed myrobalan, 20 parts of rhizoma alismatis, 15 parts of red halloysite, 35 parts of lotus seed, 35 parts of bran-fried semen coicis, 25 parts of gorgon fruit, 20 parts of fructus amomi, 35 parts of platycodon grandiflorum, 35 parts of schisandra chinensis, 35 parts of pericarpium citri reticulatae, 35 parts of nutmeg, 35 parts of scorched hawthorn fruit, 35 parts of scorched medicated leaven, 35 parts of scorched malt, 20 parts of endothelium corneum gigeriae galli, 25 parts of galangal and 35 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 120-mesh sieve after crushing, wherein the amount of sieved medicinal powder reaches 75%, and collecting sieved medicinal powder for later use; (3) putting the powder which is not sieved into water which is 12 times the weight of the powder which is not sieved, heating and refluxing for extracting for two times, wherein each time is 35 minutes, and combining and standing the extracting solutions of the two times; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum-filtering, concentrating under reduced pressure to 0.04MPa at 75 deg.C to obtain extract with relative density of 1.20 at 65 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 20.
Example 5
A traditional Chinese medicine for preventing and treating pig diarrhea comprises the following raw materials, by weight, 55 parts of codonopsis pilosula, 55 parts of poria cocos, 55 parts of bran-fried bighead atractylodes rhizome, 55 parts of Chinese yam, 55 parts of fried white hyacinth bean, 15 parts of fructus evodiae, 15 parts of stewed myrobalan, 15 parts of rhizoma alismatis, 12 parts of red halloysite, 30 parts of lotus seed, 30 parts of bran-fried semen coicis, 20 parts of gordon euryale seed, 15 parts of fructus amomi, 30 parts of platycodon grandiflorum, 30 parts of schisandra chinensis, 30 parts of dried orange peel, 30 parts of nutmeg, 30 parts of scorched hawthorn fruit, 30 parts of scorched medicated leaven, 30 parts of scorched malt, 15 parts of endothelium corneum gigeriae galli, 20 parts of galangal and 30 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 100-mesh sieve after crushing, wherein the amount of sieved medicinal powder reaches 88%, and collecting sieved medicinal powder for later use; (3) putting the powder which is not sieved into water which is 9 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 40 minutes, combining the two extracting solutions and standing; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum-filtering, concentrating under reduced pressure to 0.05MPa, maintaining the temperature at 72 deg.C, and concentrating to obtain extract with relative density of 1.20 at 64 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 18.
Example 6
A traditional Chinese medicine for preventing and treating pig diarrhea comprises the following raw materials, by weight, 50 parts of codonopsis pilosula, 60 parts of poria cocos, 50 parts of bran-fried bighead atractylodes rhizome, 60 parts of Chinese yam, 50 parts of fried white hyacinth bean, 20 parts of fructus evodiae, 10 parts of stewed myrobalan, 20 parts of rhizoma alismatis, 10 parts of red halloysite, 35 parts of lotus seed, 25 parts of bran-fried semen coicis, 25 parts of gordon euryale seed, 10 parts of fructus amomi, 35 parts of platycodon grandiflorum, 25 parts of schisandra chinensis, 35 parts of dried orange peel, 25 parts of nutmeg, 35 parts of scorched hawthorn fruit, 25 parts of scorched medicated leaven, 35 parts of scorched malt, 10 parts of endothelium corneum gigeriae galli, 25 parts of galangal and 25 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 100-mesh sieve after crushing, wherein the amount of the sieved medicinal powder reaches 91%, and collecting the sieved medicinal powder for later use; (3) putting the powder which is not sieved into water which is 11 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 40 minutes, combining the two extracting solutions and standing; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum-filtering, concentrating under reduced pressure to 0.03MPa, maintaining the temperature at 70 deg.C, and concentrating to obtain extract with relative density of 1.20 at 68 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 17.
Example 7
A traditional Chinese medicine for preventing and treating pig diarrhea comprises, by weight, 58 parts of codonopsis pilosula, 52 parts of poria cocos, 59 parts of white atractylodes rhizome stir-fried with bran, 53 parts of Chinese yam, 57 parts of white hyacinth bean stir-fried, 11 parts of fructus evodiae, 19 parts of myrobalan simmered, 12 parts of rhizoma alismatis, 15 parts of red halloysite, 26 parts of lotus seed, 33 parts of semen coicis stir-fried with bran, 15 parts of gorgon fruit, 19 parts of fructus amomi, 26 parts of platycodon grandiflorum, 35 parts of schisandra chinensis, 27 parts of pericarpium citri reticulatae, 33 parts of netmeg, 25 parts of scorched hawthorn fruit, 34 parts of scorched medicated leaven, 26 parts of scorched malt, 10 parts of endothelium corneum gigeriae galli, 25 parts of galangal and 27 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 100-mesh sieve after crushing, wherein the amount of the sieved medicinal powder reaches 93%, and collecting the sieved medicinal powder for later use; (3) putting the powder which is not sieved into water which is 10 times of the weight of the powder which is not sieved, heating and refluxing for extracting for two times, wherein each time is 35 minutes, and combining and standing the extracting solutions of the two times; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum-filtering, concentrating under reduced pressure to 0.05MPa, maintaining the temperature at 80 deg.C, and concentrating to obtain extract with relative density of 1.20 at 62 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 19.
Example 8
A traditional Chinese medicine for preventing and treating pig diarrhea comprises, by weight, 52 parts of codonopsis pilosula, 58 parts of poria cocos, 51 parts of white atractylodes rhizome stir-fried with bran, 50 parts of Chinese yam, 59 parts of white hyacinth bean stir-fried, 10 parts of fructus evodiae, 11 parts of myrobalan stewed with meat, 20 parts of rhizoma alismatis, 11 parts of red halloysite, 26 parts of lotus seed, 35 parts of semen coicis stir-fried with bran, 15 parts of gorgon fruit, 11 parts of fructus amomi, 35 parts of platycodon grandiflorum, 26 parts of schisandra chinensis, 25 parts of dried orange peel, 34 parts of nutmeg, 26 parts of scorched hawthorn fruit, 26 parts of scorched medicated leaven, 34 parts of scorched malt, 11 parts of endothelium corneum gigeriae galli, 16 parts of galangal and 34 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 120-mesh sieve after crushing, wherein the amount of sieved medicinal powder reaches 76%, and collecting the sieved medicinal powder for later use; (3) putting the powder which is not sieved into water which is 11 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 40 minutes, combining the two extracting solutions and standing; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum concentrating after suction filtration to 0.06MPa at 75 deg.C to obtain extract with relative density of 1.20 at 67 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 16.
Example 9
A traditional Chinese medicine for preventing and treating pig diarrhea comprises, by weight, 56 parts of codonopsis pilosula, 52 parts of poria cocos, 59 parts of white atractylodes rhizome stir-fried with bran, 50 parts of Chinese yam, 50 parts of stir-fried white hyacinth bean, 13 parts of fructus evodiae, 16 parts of myrobalan, 18 parts of rhizoma alismatis, 12 parts of red halloysite, 33 parts of lotus seed, 28 parts of coix seed stir-fried with bran, 20 parts of gordon euryale seed, 11 parts of fructus amomi, 29 parts of platycodon grandiflorum, 33 parts of schisandra chinensis, 31 parts of dried orange peel, 29 parts of nutmeg, 27 parts of scorched hawthorn fruit, 26 parts of scorched medicated leaven, 28 parts of scorched malt, 16 parts of endothelium corneum gigeriae galli, 21 parts of galangal and 32 parts of liquorice.
The preparation method of the traditional Chinese medicine for preventing and treating porcine diarrhea comprises the following steps of (1) weighing raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving with a 120-mesh sieve after crushing, and collecting sieved medicinal powder for later use, wherein the amount of medicinal powder passing through the sieve reaches 79%; (3) putting the powder which is not sieved into water which is 9 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 45 minutes, combining the two extracting solutions and standing; (4) combining the two extracting solutions obtained in the step (3), and using suction filtration equipment, wherein the pressure during suction filtration is 0.1 MPa; vacuum-filtering, concentrating under reduced pressure to 0.04Pa, maintaining at 75 deg.C, and concentrating to relative density of 1.20 at 63 deg.C; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea comprises enterobacter peptide and the traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1: 18.
Experiment of
Pharmacological toxicity test
I. Acute toxicity test: acute toxicity experiments using mice show that: the antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea prepared in the embodiment 2 of the invention is orally taken by mice, under the dose of 488.8g crude drugs/kg, the slight activity of the mice is reduced after administration, the mice recover to normal after about 1 hour, no animal dies after continuous observation for 7 days after administration, the general condition, diet, water intake, urination and weight increase of the animals are all normal, and the experimental result shows that: the maximum dose of the compound preparation of the invention fed by oral administration to mice is 488.8g crude drug/kg/d (LD 50>488.8g crude drug/kg). The total daily clinical medication amount of the compound preparation is O.1g crude drugs/kg/d; the medicine has low acute toxicity and safe clinical medication.
The animals were observed for changes in appearance, general behavior, food intake, and body weight during the test period, and examined for indices of hematology (RBC, HB, reticulocyte, PLT, CT, WBC, and classification) and blood biochemistry (AST, ALT, ALP, Glu, BUN, Crea, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficients, histopathology, etc. 90 days after administration and 2 weeks after discontinuation.
The test result shows that: the compound preparation of the invention has good general state in high, medium and low dose animals, and has no abnormal change in appearance physical sign, behavior activity, food intake and weight growth; the three dose groups and the control group are in normal range of hematology examination, blood biochemistry examination and urine biochemistry examination, and have no obvious difference among the groups; no obvious abnormality is found in histopathological examination of main organs in each group, and the indexes are not changed after the medicine is stopped for 2 weeks. The test dosage is 180, 60 and 20 times of the clinical dosage, the continuous administration of the compound preparation for 90 days at high, medium and low dosages, namely 18.0g crude drug/kg, 6.0g crude drug/kg and 2.Og crude drug/kg, has no obvious influence on rats, has no specific toxic target organs and sensitive indexes, and has no delayed toxic reaction even in the recovery period, namely two weeks after stopping the administration, thereby prompting that the compound preparation has higher safety of clinical application dosage.
Second, the application tests the influence of the antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating porcine diarrhea on porcine diarrhea
Diagnostic criteria for porcine diarrhea
1. The clinical symptoms comprise diarrhea of sick pigs, body temperature rise, mental depression, cold ears and nose, thin nasal discharge, fear of cold and tired lying, appetite reduction or abominability, daily diarrhea for more than 10 times, stinking water-like feces excretion, vomiting, high dehydration of organisms, emaciation and death.
2. And (3) autopsy lesions, such as post-mortem abdominal tympany, intestinal wall relaxation, gastrointestinal mucosa congestion, full stomach content, thin and full gas intestinal content, mesenteric lymph node swelling and juicy cut surface.
Hereinafter, the antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating porcine diarrhea prepared by the application is referred to as an antibacterial peptide and Chinese herbal medicine composite preparation for short, and the traditional Chinese medicine for preventing and treating porcine diarrhea prepared by the application is referred to as a Chinese herbal medicine prescription for short.
Test of influence of compound preparation of enterobacter peptide and Chinese herbal medicine added into daily ration on production performance and diarrhea of weaned piglets
A single-factor completely random design is adopted, healthy 30-day-old white piglets are selected, 180 piglets are selected, 12 piglets in each group are randomly divided into 15 groups, and the test period is 15 days. The conditions were the same for each group except for the daily dose. The administration of each group is shown in table 1 below.
The results of the various groups are shown in Table 2 below.
By comparing table 1 and table 2, the average daily gains of the test 1 group, the test 3 group, the test 4 group, the test 5 group, the test 7 group, the test 9 group and the test 11 group which are fed with the enterobacter peptide and the test 13 group which is only added with the enterobacter peptide, and the test 2 group, the test 6 group, the test 8 group, the test 10 group and the test 12 group which are corresponding to the Chinese herbal medicine formula prepared by the same example, and the test 14 group and the control group of the colistin sulfate are respectively higher than that of the basic ration by 40g, 47g, 20g, 13g, 38g, 27g, 33g, 66g and 86g, and the feed-meat ratio is respectively reduced by 0.417, 0.383, 0.168, 0.111, 0.351, 0.236, 0.351, 0.316, 0.383 and 0.805. Therefore, the influence of the enterobactin peptide and Chinese herbal medicine composite preparation on the production performance and diarrhea of weaned piglets is obviously better than that of the enterobactin peptide, Chinese herbal medicine formula and 10% colistin sulfate which are fed separately, and the effect of the enterobactin peptide and Chinese herbal medicine composite preparation prepared in the embodiment 2 is optimal. Among the Chinese herbal medicine prescription sets, the Chinese herbal medicine prescription set prepared in example 2 is the most effective.
(II) test of influence of composite preparation of enterobacter peptide and Chinese herbal medicine added into daily ration on reproductive performance of sows
40 long white X approximately gram multiparous sows with close mating time, approximately same gestation times, approximately similar weight and good health condition are randomly selected and divided into two groups, each group has 4 repetitions, and each repetition has 5 repetitions. The test is divided into 2 groups, 1 group is a control group, and basic daily ration is fed; the treatment group 2 was a test group, and the pig farm was fed 5000 mg/kg of the enterobacter peptide and Chinese herbal medicine composite preparation prepared in ten examples 2 of the basic ration. Before the test, the sows are driven to a positioning fence for feeding, the pre-feeding period is 7d, and the test is completed from 90 d of sow pregnancy to the end of piglet weaning. The results of the effect of the enterobactin-Chinese herbal medicine compound preparation on the productivity of sows are shown in table 3 below.
Note: the data in the same row shows significant difference (P < 0.05) due to different shoulder-marked letters: the same indicates that the difference does not show the potato (P > 0.05)
(III) application case
160 piglets of the same pig farm, which are aged 6 days and have similar weight, are selected, 10 piglets in each group are randomly divided into 16 groups, and the test period is 5 days. The medicine is applied 1 time in the afternoon on day 5, 13 and twice a day from day 5, 14 until no diarrhea occurs in piglets. The conditions were the same for each group except for the daily dose. The administration and treatment effects of each group are shown in Table 4 below.
Through the experimental results of table 4, the enterobactin-Chinese herbal medicine composite preparation has a remarkable treatment effect on piglet dysentery, and the curative effect is obviously superior to that of the enterobactin-Chinese herbal medicine, norfloxacin and furazolidone which are singly used. The treatment effect of the enterobactin peptide and Chinese herbal medicine composite preparation prepared in the example 2 on piglet dysentery is optimal, and the Chinese herbal medicine formula prepared in the example 2 is optimal in the Chinese herbal medicine formula group.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various changes and modifications can be made without departing from the overall concept of the present invention, and these should also be considered as the protection scope of the present invention.
Claims (4)
1. An antibacterial peptide and Chinese herbal medicine compound preparation for preventing and treating pig diarrhea is characterized in that: comprises enterobacter peptide and traditional Chinese medicine for preventing and treating pig diarrhea, wherein the weight ratio of the enterobacter peptide to the traditional Chinese medicine is 1 (15-20); the traditional Chinese medicine for preventing and treating pig diarrhea comprises, by weight, 50-60 parts of codonopsis pilosula, 50-60 parts of poria cocos, 50-60 parts of bran-fried bighead atractylodes rhizome, 50-60 parts of Chinese yam, 50-60 parts of fried white hyacinth bean, 10-20 parts of fructus evodiae, 10-20 parts of stewed myrobalan, 10-20 parts of rhizoma alismatis, 10-15 parts of halloysite, 25-35 parts of lotus seed, 25-35 parts of bran-fried semen coicis, 15-25 parts of semen euryales, 10-20 parts of fructus amomi, 25-35 parts of platycodon grandiflorum, 25-35 parts of schisandra chinensis, 25-35 parts of pericarpium citri reticulatae, 25-35 parts of netmeg, 25-35 parts of scorched hawthorn fruit, 25-35 parts of scorched medicated leaven, 25-35 parts of scorched malt, 10-20 parts of chicken, 15-25 parts of rhizoma zingiberis and 25-35 parts of liquorice.
2. The antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating porcine diarrhea according to claim 1, characterized in that: the traditional Chinese medicine for preventing and treating pig diarrhea comprises, by weight, 60 parts of codonopsis pilosula, 60 parts of poria cocos, 60 parts of bran-fried bighead atractylodes rhizome, 60 parts of Chinese yam, 60 parts of fried white hyacinth bean, 15 parts of fructus evodiae, 15 parts of stewed myrobalan, 15 parts of rhizoma alismatis, 10 parts of red halloysite, 30 parts of lotus seed, 30 parts of bran-fried semen coicis, 20 parts of gordon euryale seed, 15 parts of fructus amomi, 30 parts of platycodon grandiflorum, 30 parts of schisandra chinensis, 30 parts of pericarpium citri reticulatae, 30 parts of nutmeg, 30 parts of scorched hawthorn fruit, 30 parts of scorched medicated leaven, 30 parts of scorched malt, 15 parts of endothelium corneum gigeriae galli, 20 parts of galangal and 30 parts of liquorice.
3. The antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating porcine diarrhea according to claim 1, characterized in that: the preparation method of the traditional Chinese medicine for preventing and treating the porcine diarrhea comprises the following steps of (1) weighing the raw materials according to the weight ratio; (2) mixing the weighed raw materials, crushing, sieving by a 100-120-mesh sieve after crushing, enabling the amount of the medicine powder passing through the sieve to be more than 70%, and collecting the sieved medicine powder for later use; (3) putting the powder which is not sieved into water which is 8-12 times of the weight of the powder which is not sieved, heating and refluxing for extraction twice, each time for 30-45 minutes, and merging and standing the extracting solutions of the two times; (4) combining the two extracting solutions obtained in the step (3), using suction filtration equipment, wherein the pressure during suction filtration is 0.1MPa, performing reduced pressure concentration after suction filtration, reducing the pressure to 0.03-0.06 MPa, keeping the temperature at 70-80 ℃, and concentrating to obtain an extract with the relative density of 1.20 at 60-70 ℃; (5) spray drying the extract with spray drying equipment at air inlet temperature of 160 deg.C and air outlet temperature of 90 deg.C, and collecting spray dried powder; and (2) mixing the sieved medicinal powder collected in the step (1) to obtain the prepared traditional Chinese medicine for preventing and treating porcine diarrhea.
4. The antibacterial peptide and Chinese herbal medicine composite preparation for preventing and treating porcine diarrhea according to claim 1, characterized in that: the weight ratio of the enterobactin peptide to the traditional Chinese medicine is 1: 15.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785396A (en) * | 2005-07-28 | 2006-06-14 | 江西汇仁药业有限公司 | Zisheng fast dispersion solid preparation and its preparation method |
| CN103394057A (en) * | 2013-07-24 | 2013-11-20 | 赵世明 | Traditional Chinese medicine composition for treating diarrhoea |
| CN104548015A (en) * | 2014-12-29 | 2015-04-29 | 平阴县人民医院 | Medicine for treating chronic colitis |
| CN104857501A (en) * | 2015-06-08 | 2015-08-26 | 信阳农林学院 | Chinese and western medicine composition for treating piglet diarrhea and preparation method thereof |
-
2018
- 2018-10-22 CN CN201811226899.3A patent/CN109316598B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785396A (en) * | 2005-07-28 | 2006-06-14 | 江西汇仁药业有限公司 | Zisheng fast dispersion solid preparation and its preparation method |
| CN103394057A (en) * | 2013-07-24 | 2013-11-20 | 赵世明 | Traditional Chinese medicine composition for treating diarrhoea |
| CN104548015A (en) * | 2014-12-29 | 2015-04-29 | 平阴县人民医院 | Medicine for treating chronic colitis |
| CN104857501A (en) * | 2015-06-08 | 2015-08-26 | 信阳农林学院 | Chinese and western medicine composition for treating piglet diarrhea and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| 刘国强.资生丸治疗肠道易激综合征80例.《国医论坛》.2003,第18卷(第02期),第22页左栏第2段. * |
| 益气健脾清肠固涩法治疗腹泻型肠易激综合征60例;邱智等;《中国中医药科技》;20060930;第13卷(第05期);第363-364页 * |
| 肠安汤治疗肠易激综合征56例;李景花;《浙江中医杂志》;20080716;第43卷(第07期);第401页 * |
| 资生丸治疗肠道易激综合征80例;刘国强;《国医论坛》;20030331;第18卷(第02期);第22页左栏第2段 * |
| 陈大权教授治疗泄泻验案举隅;杨爽;《中医药学报》;20131020;第41卷(第05期);第112-113页 * |
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