KR102418383B1 - Compositions for the prevention or treatment of obesity comprising natural complexes - Google Patents

Abstract

The present invention relates to a composition for preventing, improving or treating obesity, comprising a mixture of barley sprout, rice germ, goji berry, beet, brewer's yeast, ginger and cinnamon. The composition of the present invention is rich in zinc and iron, so it can prevent trophic anemia that can occur during diet, and when administered to an animal model fed a high-fat diet, it has an effect of inhibiting the increase in body fat in a concentration-dependent manner Therefore, it can be usefully used for the prevention or management of obesity.

Description

Compositions for the prevention or treatment of obesity comprising natural complexes

The present invention relates to a composition for preventing or treating obesity comprising a natural complex.

Obesity or obesity refers to the state of having excess body fat in the body, and is a generic term for the phenomenon of excessive weight. Pathologically, the so-called standard body mass index (BMI) set by the World Health Organization (WHO) Obesity is defined as a case where the body fat is more than 30.1, but usually when the body fat is 25% or more of body weight in men and 30% or more of body weight in women, obesity is diagnosed. Also, in most cases, it means that you weigh more than normal, but even if you do not weigh much, if the proportion of fat in the body composition is high, it is called obesity.

The causes of obesity include genetic factors, environmental factors, and abnormal energy metabolism, and the types of obesity can be classified into simple obesity and symptomatic obesity depending on the cause. Simple obesity is caused by overeating and lack of exercise. Symptomatic obesity is caused by endocrine, hypothalamus, heredity, frontal lobe and metabolism. Obesity may not be a problem in itself, but the secondary complications caused by social disability and excessive fat are serious. For example, obesity can significantly increase the incidence rate of hyperlipidemia, hypertension, arteriosclerosis, diabetes, fatty liver, and joint abnormalities.

As such, obesity is the cause of various adult diseases, and if you gain weight, your ability to process blood sugar decreases, which makes it easy to cause diabetes and high blood pressure. If the body weight is increased by 20% compared to the normal weight, the risk of developing diabetes increases 10 times that of a normal person. It can also lead to weakened heart and lung function, shortening lifespan. Complex factors such as high blood pressure, diabetes, hyperlipidemia, and arteriosclerosis put pressure on heart function, leading to heart failure and sudden death. Furthermore, in the case of women, the incidence of endometrial cancer increases, and the possibility of developing cholelithiasis increases, and in the case of men, the incidence of colorectal and prostate cancer increases. In addition to the onset of the disease, as we gain weight, we become overly conscious of how others look at us, and when the obsessive-compulsive obsession grows, we avoid people or try to be alone. In addition, the feeling of inferiority coming from the body affects the self by losing confidence in life.

In order to prevent such obesity and maintain a healthy weight, it is necessary to maintain a balanced diet and regular exercise throughout life. In particular, fat cells do not disappear once they are made, and it is impossible to remove them naturally, so they remain permanently in the human body.

Therefore, there are cases in which drugs such as reducing fat absorption are taken along with diet intake. However, these diet pills may cause side effects such as cardiovascular diseases such as high blood pressure and stroke, and may be accompanied by a yo-yo phenomenon in which weight increases again when taking the drug is stopped. In the case of severe obesity, not only drug therapy but also surgical therapy is performed. Surgery to remove fat or satellite surgery or gastric band insertion to limit the amount of food the body can digest is being performed, but there are also risk factors exists Accordingly, there is a demand for the development of a safe anti-obesity and diet composition without the side effects as described above.

The present inventors have completed the present invention by confirming the excellent body fat lowering effect of a natural complex prepared by combining various natural products.

Accordingly, an object of the present invention is to provide a pharmaceutical composition for preventing or treating obesity comprising a natural complex.

Another object of the present invention is to provide a method for preparing a pharmaceutical composition for preventing or treating obesity comprising a natural complex.

Another object of the present invention is to provide a food composition for preventing or improving obesity comprising a natural complex.

Another object of the present invention is to provide a composition for adding a feed for preventing or improving obesity comprising a natural complex.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating obesity comprising a mixture of barley sprouts, rice germ, goji berry, beets, brewer's yeast, ginger and cinnamon.

In addition, in order to achieve the above another object, the present invention is a method of mixing barley sprout, rice germ, goji berry, beetroot, brewer's yeast, ginger and cinnamon in a dry weight ratio of 1:1:1:1:1:1:1. step; grinding the mixed mixture; Separating only particles having an average particle size of 14 mesh or less in the pulverized product; and removing the metal of the separated particles; provides a method for producing a pharmaceutical composition for preventing or treating obesity, characterized in that it comprises a.

In addition, in order to achieve the above another object, the present invention provides a food composition for preventing or improving obesity comprising a mixture of barley sprouts, rice germ, Goji berry, beets, brewer's yeast, ginger and cinnamon.

In addition, in order to achieve the above another object, the present invention provides a composition for preventing or improving obesity comprising a mixture of barley sprouts, rice germ, Goji berry, beets, brewer's yeast, ginger and cinnamon.

The natural complex containing barley sprout, rice germ, goji berry, beetroot, brewer's yeast, ginger and cinnamon according to the present invention is rich in zinc, iron, etc. When administered to an animal model fed with a diet, it has the effect of inhibiting the increase in body fat in a concentration-dependent manner, so it can be usefully used as a pharmaceutical composition for preventing, improving or treating obesity, a food composition, or a composition for adding feed.

1 is a view showing the manufacturing process of a natural complex according to the present invention.
Figure 2 is the result of confirming the dietary intake of the animal model administered with the natural complex according to the present invention.
3 is a result confirming the change of body fat percentage (body fat percentage (%)) over time in an animal model administered with a natural compound according to the present invention.
4 is a result of confirming the body fat density of the animal model administered with the natural complex according to the present invention.
5 is a result of confirming the weight of the animal model abdominal fat (epididymal + retroperitoneal) administered with the natural complex according to the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for preventing or treating obesity, comprising a mixture of barley sprout, rice germ, goji berry, beet, brewer's yeast, ginger and cinnamon.

In the present invention, the barley sprout means a sprout of barley, and may be called sprouted barley. The barley sprout is a young leaf before barley bears fruit, and it is a treasure trove of nutrients and strong vitality that can absorb nutrients from the soil. In particular, it is reported that barley sprouts have the greatest nutrient when they are 20 cm in length, and that barley sprouts promote the growth of new cells and have therapeutic effects on skin diseases and ulcers. Barley sprouts are rich in many nutrients such as natural antioxidants, beta-carotene, biological enzymes, calcium, iron, and chlorophyll.

The rice germ (embryo) is known to contain as much as 66% of the nutrients of rice, and the nutrients contained in the rice germ are GABA (gamma amino butyric acid), gamma oryzanol, beta cysterol, alpha-tocopherol, octacosanol, dietary fiber, and linoleic acid. It contains a large amount of very important nutrients such as vitamins and minerals.

The gugija refers to the fruit of the wolfberry tree (Lycii fructus), the bark of the root is called phalangeal skin, the leaf is called gugiyeop, and the young shoot is called chungchoongcho. According to Donguibogam, goji berries are effective for nourishment, tonicity, blood, and jigal. It is recorded that it is good for diseases that paralyze the nerves and improves the eyesight by promoting the functions of the lungs and kidneys.

The beet is a root vegetable and contains a pigment called betaine, which inhibits cell damage and has an antioxidant effect that is 8 times greater than that of tomatoes, thereby preventing cancer, such as lung cancer and pneumonia, and relieving inflammation. 8% of beets are composed of chlorine, and this chlorine component is effective in purifying the liver and in the formation of bones and development of infants. It also contains a large amount of iron and vitamins, which helps to produce red blood cells and cleans the blood, which is known to be good for menstrual disorders or menopausal women. In addition, it prevents gastric damage and protects the gastric mucosa.

The brewer's yeast is a yeast isolated after filtration of beer after fermentation of wort is completed during the beer manufacturing process, and is composed of 50% protein, 10 natural vitamins, 30% essential minerals, plant fiber, nucleic acid, etc. have. It contains biotin, a vitamin B group essential for protein metabolism for hair growth, and cystine and methionine that make up hair protein. It also contains trace elements such as zinc and chromium that increase insulin activity. Rich in beta-glucan, which inhibits abnormal cell proliferation by activating immune cells of normal cells, is known to help immunity This increases, prevents or treats constipation or diarrhea, and improves intestinal absorption. In addition, it contains amino acids and nucleic acids necessary for repairing damaged gastrointestinal mucosa, as well as other nutrients, so it is effective for gastrointestinal diseases.

The ginger (Zingiber officinale) is the root of ginger (Zingiber officinale Roscoe), a perennial vegetable of the ginger family (Zingiberaceae) native to tropical Asia, widely distributed in Southeast Asia and the like, and has been used as a medicinal agent according to folk remedies. Starch accounts for 40 to 60% of the total amount of ginger, and there are aromatic ingredients, resin protein, fiber, pentosan, and minerals. The pungent taste of ginger is zingeron, gingerol, About 40 species are known, such as shogaol, dihydrogingerol, and citral and camphene as aromatic ingredients. As a study on the activity of ginger, antioxidant, anti-inflammatory, insecticidal, and uterine relaxing effects have been reported.

The cinnamon (cinnamon) is a medicinal agent made from the bark of the evergreen tree belonging to the camphor family, Saengdal tree (天竹桂, Cinnamomum japonicum SIEB.). The dried bark is curved or semi-curled and forms irregular pieces. The thickness is usually 3 mm, and the color of the outer part is black-brown or black-red, and there are spots like gray-white flowers. The inside is dark red to dark red. The outer layer of the cut surface is grayish-brown. It is mainly grown in southern China, southern Japan, Vietnam, Cambodia and Thailand. The active ingredient is about 1% of the volatile essential oil component, and among them, Phellandrene, Eugenol, Methyleugenol, and the like are contained.

The natural complex according to the present invention is characterized by mixing the barley sprout, rice germ, goji berry, beetroot, brewer's yeast, ginger and cinnamon in a dry weight ratio of 1:1:1:1:1:1:1.

The barley sprout, rice germ, goji berry, beet, ginger and cinnamon may be used in a state containing moisture, that is, as herbal medicines, or dried. Alternatively, in order to increase the content of the retained physiologically active substances, it can be used by steaming by a method known in the art. The steaming is a process of steaming and drying herbal medicines by steam or other methods, and is one of heat treatment methods for the purpose of long-term storage and distribution. In oriental medicine, it has been used as one of the methods to reduce toxicity and minimize side effects through heat treatment of herbal medicines to increase the therapeutic effect, and to conveniently use it for processing or sectioning.

In particular, the natural complex according to the invention is characterized in that it contains folic acid. The natural complex according to the present invention contains folic acid that is significantly higher than the standard value (100 μg) of known weight control products based on the same weight, that is, 1000 μg or more, preferably 4000 μg or more. It is possible to prevent nutritional anemia, which may occur due to a decrease in city intake.

In addition, the natural composite according to the present invention is characterized in that the average particle size is 14 mesh or less.

When the average particle size of the natural composite according to the present invention exceeds the 14 mesh, the user may feel that the particles are rough when ingested, which may affect the intake.

The pharmaceutical composition of the present invention may contain the natural complex alone, or may additionally contain one or more pharmaceutically acceptable carriers, excipients or diluents.

The pharmaceutically acceptable carrier may further include, for example, a carrier for oral administration or a carrier for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like.

In addition, the carrier for parenteral administration may include water, a suitable oil, saline, aqueous glucose and glycol, and the like, and may further include a stabilizer and a preservative. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives are benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. As other pharmaceutically acceptable carriers, reference may be made to those described in the following literature (Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995).

The pharmaceutical composition of the present invention may be administered to mammals, including humans, by any method, preferably orally, and may be administered parenterally, including enteral or rectal administration.

The pharmaceutical composition of the present invention may be formulated as a formulation for oral administration or parenteral administration according to the administration route as described above.

In the case of a formulation for oral administration, the composition of the present invention may be formulated as a powder, granule, tablet, pill, dragee, capsule, liquid, gel, syrup, slurry, suspension, etc. using methods known in the art. can For example, oral preparations can be obtained by mixing the active ingredient with a solid excipient, grinding it, adding suitable adjuvants, and processing it into a granule mixture to obtain tablets or dragees. Examples of suitable excipients include sugars, including lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol and maltitol, and starches, including corn starch, wheat starch, rice starch and potato starch, cellulose, Cellulose, including methyl cellulose, sodium carboxymethylcellulose and hydroxypropylmethyl-cellulose, and the like, fillers such as gelatin, polyvinylpyrrolidone, and the like may be included. In addition, cross-linked polyvinylpyrrolidone, agar, alginic acid or sodium alginate may be added as a disintegrant if necessary. Furthermore, the pharmaceutical composition of the present invention may further include an anti-aggregating agent, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent, and an antiseptic agent.

Formulations for parenteral administration may be formulated in the form of injections, creams, lotions, external ointments, oils, moisturizers, gels, aerosols and nasal inhalants by methods known in the art. These formulations are described in Remington's Pharmaceutical Science, 15th Edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a recipe commonly known to all pharmaceutical chemistry.

The total effective amount of the pharmaceutical composition of the present invention may be administered to a patient as a single dose, and may be administered by a fractionated treatment protocol in which multiple doses are administered for a long period of time. . The pharmaceutical composition of the present invention may vary the content of the active ingredient depending on the severity of the disease. When administered parenterally, it is preferably administered in an amount of 0.01 to 50 mg, more preferably 0.1 to 30 mg per kg of body weight per day based on the natural complex, and when administered orally, based on the natural complex per day It can be administered in 1 to several divided doses so that it is preferably administered in an amount of 0.01 to 100 mg, more preferably 0.1 to 50 mg per 1 kg of body weight. However, the dose of the natural complex is determined by considering various factors such as the age, weight, health status, sex, severity of disease, diet and excretion rate of the patient, as well as the administration route and number of treatments of the pharmaceutical composition, and the effective dosage for the patient is determined. Therefore, in consideration of this point, those of ordinary skill in the art will be able to determine an appropriate effective dosage of the composition of the present invention. The pharmaceutical composition according to the present invention is not particularly limited in its formulation, administration route and administration method as long as the effect of the present invention is exhibited.

In addition, the present invention comprises the steps of mixing barley sprout, rice germ, goji berry, beetroot, brewer's yeast, ginger and cinnamon in a dry weight ratio of 1:1:1:1:1:1:1; grinding the mixed mixture; Separating only particles having an average particle size of 14 mesh or less in the pulverized product; and removing the metal of the separated particles. It provides a method for preparing a pharmaceutical composition for preventing or treating obesity, comprising:

The removal of the metal may be performed using a magnetic force of 10,000 Gauss or more, but is not limited thereto.

In addition, after the step of removing the metal of the separated particles, it may further include a sterilization step. The sterilization may be performed at a temperature of 100 to 180° C. and a pressure of 0.1 to 0.4 Mpa, more preferably at a temperature of 150 to 160° C., at a pressure of 0.2 to 0.3 Mpa, but is not limited thereto.

In addition, the present invention provides a food composition for preventing or improving obesity, comprising a mixture of barley sprouts, rice germ, goji berries, beets, brewer's yeast, ginger and cinnamon.

The food composition according to the present invention includes all types of functional food, nutritional supplement, health food, and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

More preferably, it can be prepared in the form of a meal replacement for suppressing the increase in body weight and body fat.

For example, as a health food, the natural complex itself of the present invention may be ingested by granulating, encapsulating and powdering, or it may be prepared and consumed in the form of tea, juice or drink. In addition, the natural complex of the present invention can be prepared in the form of a composition by mixing it with known substances or active ingredients known to have an effect on improving or treating obesity.

In addition, functional foods include beverages (including alcoholic beverages), fruits and their processed foods (eg, canned fruit, bottled, jam, marmalade, etc.), fish, meat, and their processed foods (eg, ham, sausage corn beef, etc.) , Breads and noodles (eg udon noodles, noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, syrup, dairy products (eg butter, cheese, etc.), edible vegetable oils and fats, margarine, vegetable protein, retort It can be prepared by adding the natural complex of the present invention to food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.).

The preferred content of the natural complex of the present invention in the food composition of the present invention is not limited thereto, but may be, for example, 0.01 to 80% by weight of the finally prepared food, preferably 0.01 to 80% by weight of the finally prepared food 50% by weight.

In addition, in order to use the natural complex of the present invention in the form of a food additive, it may be prepared and used in the form of a powder or a concentrate.

In addition, the present invention provides a composition for adding a feed for preventing or improving obesity, comprising a mixture of barley sprouts, rice germ, goji berry, beets, brewer's yeast, ginger and cinnamon.

The term "feed" of the present invention means any natural or artificial Chinese New Year meal, one meal meal, etc. eaten by animals, or a component of the one meal meal, and a feed comprising the composition for preventing or improving obesity according to the present invention as an active ingredient can be prepared in various types of feed known in the art, and preferably may include a concentrated feed, roughage and / or special feed, but is not limited thereto.

In the present invention, the term "composition for feed addition" may mean "feed additive", which in animals relieves disease symptoms, supplements nutrients and prevents weight loss, enhances digestibility of fiber in feed, improves oil quality, breeding It includes substances added to feed for the purpose of various effects, such as preventing disorders or improving fertility, and preventing high temperature stress in summer.

The composition for feed addition of the present invention corresponds to an auxiliary feed under the Feed Management Act, and mineral preparations such as sodium bicarbonate, bentonite, magnesium oxide, and complex minerals, and trace minerals such as zinc, copper, cobalt, and selenium. , kerotene, vitamins A, D, E, nicotinic acid, vitamin B complex, etc., protective amino acids such as methionine and lysine, protective fatty acids such as fatty acid calcium salts, probiotics (lactic acid bacteria), yeast cultures, mold fermented products, etc. Live bacteria, yeast agents, etc. may be further included.

Among the feeds, the enriched feed includes seed fruits containing grains such as wheat, oats, and corn, bran containing rice bran, bran, barley bran, etc. Sesame cakes, which are by-products obtained from oil extraction of palms, etc., residual starches, which are the main components of starch residues, which are the remainder after removing starch from sweet potatoes and potatoes, fish meal, fish residues, and fresh liquids obtained from fish. Animal substances such as dried whey, which is the residue from the production of concentrated fish soluble, meat meal, blood meal, down feather, skim milk powder, milk, and casein from skim milk Feed, yeast, chlorella, seaweed, but not limited thereto.

Among the feeds, forage includes raw grass feed such as wild grass, grass, green cut crops, turnips for feed, beets for feed, root vegetables such as Luther Bearger, which is a type of turnip, raw herbs, green crops, and grains. Examples include, but are not limited to, silage, which is stored feed fermented with lactic acid after filling a silo with a back, wild grass, hay obtained by cutting and drying grass, straw for breeding crops, and leaves of legumes. Special feed includes mineral feed such as oyster shells and rock salt, urea feed such as urea or its derivative diureide isobutane, supplementing ingredients that are easily lacking when only natural feed raw materials are mixed, or added to formulated feed to increase feed storage. There are feed additives and dietary supplements, which are substances added in trace amounts, but are not limited thereto.

The composition for feed addition according to the present invention can be prepared by adding a natural compound in an appropriate effective concentration range according to various feed preparation methods known in the art.

The feed additive according to the present invention can be applied without limitation as long as it is an object for the purpose of preventing or improving obesity. For example, non-human animals such as monkeys, dogs, cats, rabbits, guinea pigs, rats, mice, cattle, sheep, pigs, goats, etc., birds and fish can be applied to any object.

The above-described contents of the present invention are equally applied to each other as long as they do not contradict each other, and those skilled in the art to implement with appropriate changes are also included in the scope of the present invention.

Hereinafter, the present invention will be described in detail through Examples, but the scope of the present invention is not limited only to the Examples below.

Example 1. Preparation of natural complexes

The natural complex according to the present invention was prepared according to the steps shown in FIG. 1 . First, a mixture was prepared by mixing barley sprout, rice germ, goji berry, red beet, brewer's yeast, ginger and cinnamon in a dry weight ratio of 1:1:1:1:1:1:1.

After the mixed mixture was pulverized, foreign substances were removed using a filtration network of 14 mesh (mesh, 1.5 mm), and only particles having a particle size of 14 mesh or less were separated. Then, the iron powder in the mixture was removed with a magnetic force of 10,000 Gauss or more. After completing the above process, the mixture was sterilized at a temperature of 150 to 160° C. and a pressure of 0.24 to 0.28 Mpa, and stored refrigerated. After dissolving the prepared natural complex in distilled water (DW), different concentrations were prepared (high concentration group: 5,000 mg/kg, medium concentration group: 2,500 mg/kg, low concentration group: 1,250 mg/kg). In addition, as a positive control, orlistat was completely dissolved in dimethyl sulfoxide (DMSO) and an excipient, and then diluted with DW to prepare a concentration of 30 mg/kg.

Example 2. Identification of nutritional components of natural complexes

The nutritional components of the natural complex according to the present invention were confirmed as follows (one time (50 g) intake standard).

2.1 Caloric value of natural complexes

Multiply the content of crude protein, crude fat and carbohydrate or carbohydrate in 100 g of natural complex by the coefficients of protein 4, fat 9, and carbohydrate 4 using Atwater factor to calculate each energy in kilocalorie (Kcal) unit, expressed as the total. The units are kilocalories or kilojoules (KJ), and conversion from kilocalorie units to kilojoules was calculated to be 4.184KJ for 1Kcal.

2.2 Folic acid content in natural complexes

Folic acid contained in the natural complex according to the present invention was extracted with 10 mM phosphate buffered saline (PBS, pH 8.0), separated from liquid chromatography using a reversed-phase partitioning column, and measured at UV 280 nm.

In addition, the content of folic acid was measured according to the following equation (Y' = concentration of the test solution obtained by substituting it into the calibration curve (ug/mL), V = volume of the solution used for sample extraction (mL), S = extraction sample weight (g)).

[Equation 1]

2.3 Protein content of natural complexes

About 1 g of the natural complex according to the present invention was put in a decomposition tube, and 2 tablets of a decomposition accelerator were added. Then, 12 mL of concentrated sulfuric acid was added to the decomposition tube and decomposed for 45 to 60 minutes in a decomposition device at 420 °C. After adding 80 mL of distilled water to the decomposed solution, 25 mL of the collection solution mixed with the mixing indicator was placed in an Erlenmeyer flask, placed on the distillation apparatus, and lifted up the Erlenmeyer flask stand to allow the distillate to enter the collection solution during distillation. Then, 50 mL of 40% NaOH (an amount equivalent to 4 times the amount of sulfuric acid used for decomposition) was put into the decomposition tube, and then distilled in a distillation apparatus for 3 to 4 minutes. At this time, the collection solution in the Erlenmeyer flask of the distillation device turns green while collecting the alkali (ammonia) contained in the distillate. The distillate was titrated with hydrochloric acid solution (0.1 N or 0.2 N) until the end point reached a pale pink color. At this time, by recording the amount of acid used for titration, the total nitrogen (%) was calculated through Equation 2 below, and then the protein content was calculated through Equation 3 based on this (atomic weight of nitrogen: 14.01, M: HCl Molar concentration of, F: nitrogen coefficient (Nitrogen coefficient of 1.1.3.1 A. 5)).

[Equation 2]

[Equation 3]

2.4 Calcium, iron and zinc content in natural complexes

The natural composite according to the present invention was taken in an incineration container and carbonized, then heated at a temperature of 550 to 600° C. and incinerated until white to off-white ash was obtained. After standing to cool this ash, wet it with water, add about 10 mL of hydrochloric acid solution (1→2), and completely evaporate to dryness on a water bath. About 8-10 mL of hydrochloric acid solution (1→4) was added to this dried product, heated to water, and filtered through a 100 mL volumetric flask. The insoluble matter was transferred to a painting container used together with filter paper to dry and then incinerated again. The ash was wetted with water, about 2 mL of hydrochloric acid solution (1→4) was added, diluted with about 5 mL of water, and then heated in a water bath. Thereafter, the filtered solution was filled in the 100 mL volumetric flask, and water was added to make 100 mL, which was used as a test solution.

Calcium content was measured based on the test solution. The test solution was adjusted to have a calcium concentration of 1 to 5 μg/mL by using 1 N hydrochloric acid instead of water, and measured according to the atomic absorption spectrophotometry. Lanthanum chloride (LaCl ₃ ) was added to the 1 N hydrochloric acid solution used at this time so as to be 1,000 ppm as lanthanum (La), or strontium chloride (SrCl ₂ ) was added to be 5,000 ppm as strontium (Sr) was used. .

In addition, the iron content was measured based on the test solution. The test solution was adjusted to have an iron concentration of 1-4 μg/mL by using 1 N hydrochloric acid instead of water, and measured according to the atomic absorptiometry.

In addition, the zinc content was measured based on the test solution. The test solution was adjusted to have a zinc concentration of 1 to 10 μg/mL and measured according to atomic absorption spectroscopy or inductively coupled plasma method.

The nutritional components of the natural complex of the present invention, measured as in 2.1 to 2.3, are compared with the standard values of known weight control products (Ministry of Food and Drug Safety Food Standards and Provided, refer to the 2019 Food Standards Manual) and are shown in Table 1 below. it was

nutrient weight control products
standard number natural complex levels calorie 200 ~ 400Kcal 203Kcal folic acid 100 μg or more 5169μg protein 5.5g or more 6.53g calcium 70mg or more 150mg steel 1.2mg or more 4.29mg zinc 0.85mg or more 2.15mg

According to Table 1, the natural complex of the present invention was suitable for the standard values of known weight control products, and in particular, folic acid showed significantly higher values than the standard values. As nutritional anemia may occur due to a decrease in intake during dietary adjustment, the natural complex of the present invention is rich in zinc, iron, and the like, so it is determined that the occurrence of nutritional anemia can be prevented when ingested.

Example 3. Preparation of animal models

In order to confirm the effect of the natural complex of the present invention in an animal model, the following experimental groups were prepared.

5-week-old SD rats (Rat) were fed a regular diet and a high-fat (60% fat-containing) diet. Feed and water were freely ingested during the experiment, and the temperature of the breeding room was 23±2℃, the humidity was 50±10%, and the light and dark cycle was maintained for 12 hours.

In addition, the experimental group consisted of six groups as follows: 10 normal group (Normal) administered with distilled water on a regular diet, 10 obese control animals administered with distilled water on a high-fat diet (Control), and a group administered with high concentration of natural complex on a high-fat diet 10 animals (NK booster 5000 mg/kg), 10 animals in the natural complex medium dose group on a high fat diet (NK booster 2500 mg/kg), 10 animals in the natural complex low dose group on a high fat diet (NK booster 1250 mg/kg), high fat diet 10 positive control rats administered with 30 mg/kg of orlistat (Positive control). According to the conditions of each experimental group, oral administration, twice a day, was performed for a total of 8 weeks.

Example 4. Analysis of dietary intake

By confirming the dietary intake of the experimental group, the change in dietary intake according to the intake of the natural complex of the present invention was confirmed. The dietary intake was calculated by subtracting the remaining amount the next day from the feed amount provided for one week during the total 8-week experimental period.

As a result, as shown in FIG. 2 , it was confirmed that the dietary intake decreased when the natural complex according to the present invention was ingested compared to the Control experimental group. In particular, it was confirmed that the dietary intake of the medium and high concentration administration group was significantly reduced.

Example 5. Body fat percentage analysis

The body fat percentage of each experimental group of Example 2 was checked and compared. To this end, the body fat mass of each experimental group was confirmed using a nuclear magnetic resonance device (EchoMRITM-700, USA). Based on the confirmed body fat mass, the body fat percentage was calculated as follows.

[Equation 4]

Body fat percentage (%) = (body fat mass / body weight × 100)

The percentage of body fat is expressed as a percentage (%) of the weight of body fat in the total body weight, and is a major indicator for evaluating the efficacy of body fat reduction.

As a result, as shown in FIG. 3 , in all of the experimental groups administered with the natural complex according to the present invention, the increase in body fat percentage by the high-fat diet over time was smaller than that of the Control group. In particular, it was confirmed that, in the case of the high concentration of natural complex administration group, the increase in body fat percentage due to the high fat diet was significantly smaller compared to the results of the Control group.

Example 6. Body fat density analysis

In addition, the body fat density of each experimental group was analyzed. After respiratory anesthesia of the rats of each experimental group at week 8 using Isoflurane, the body fat density was confirmed as an image using Dual energy X-ray absorptiometry (DXA). In the results, high-lipids can be visually confirmed in red.

As a result, as shown in FIG. 4 , it was confirmed that all of the experimental groups administered with the natural complex according to the present invention had significantly lower body fat density compared to the control group. In particular, in the case of the high concentration administration group of the natural complex, it was confirmed that the high-lipid content in the body was locally distributed very little. In particular, in the case of the medium concentration of natural complex administration group, the distribution of high lipids was similar to that of the positive control group, and in the case of the high concentration administration group, it was confirmed that the distribution of high lipids was smaller than that of the positive control group. Therefore, similarly to the results of Example 5, it was confirmed that the natural complex according to the present invention had an effect of reducing body fat.

Example 7. Confirmation of changes in abdominal fat weight

In addition, the weight was measured by extracting the epigastric and retroperitoneal fat from the rats of each experimental group at week 8. As a result, as shown in FIG. 5 , it was confirmed that all of the experimental groups administered the natural complex according to the present invention had a lighter fat weight than the control group, and lighter fat weight compared to the positive control group. In particular, in the case of the high concentration of natural complex administration group, compared to other experimental groups, the weight of fat was significantly lighter and it was confirmed that it was similar to the normal experimental group.

Overall, the present invention confirms the anti-obesity effect of the natural complex, and the natural complex according to the present invention contains a mixture of barley sprout, rice germ, goji berry, beet, brewer's yeast, ginger and cinnamon, and is rich in zinc, iron, etc. Therefore, it is possible to prevent nutritional anemia that can occur during diet, and when administered to an animal model fed with a high-fat diet, it showed an effect of inhibiting the increase in body fat in a concentration-dependent manner. It can be usefully used as a food composition or a composition for adding feed.

Claims (9)

A pharmaceutical composition for preventing or treating obesity, comprising a mixture of barley sprouts, rice germ, goji berry, beets, brewer's yeast, ginger and cinnamon.
The method of claim 1,
The mixture is a pharmaceutical composition, characterized in that the mixture of barley sprout, rice germ, goji berry, beet, brewer's yeast, ginger and cinnamon in a dry weight ratio of 1:1:1:1:1:1:1.
The method of claim 1,
The pharmaceutical composition, characterized in that the mixture comprises folic acid.
The method of claim 1,
The mixture is characterized in that the average particle size is 14 mesh or less, the pharmaceutical composition.
mixing barley sprout, rice germ, goji berry, beetroot, brewer's yeast, ginger and cinnamon in a dry weight ratio of 1:1:1:1:1:1:1;
grinding the mixed mixture;
Separating only particles having an average particle size of 14 mesh or less in the pulverized product; and
Removing the metal of the separated particles; Method for producing a pharmaceutical composition for preventing or treating obesity, comprising a.
6. The method of claim 5,
The removal of the metal is characterized in that performed by using a magnetic force of 10000 Gauss or more, the manufacturing method.
Barley sprout, rice bran, goji berry, beet, brewer's yeast, ginger, and a food composition for preventing or improving obesity comprising a mixture of cinnamon.
8. The method of claim 7,
The composition is a food composition, characterized in that it is a meal replacement for suppressing the increase in body weight and body fat.
Barley sprout, rice bran, goji berry, beet, brewer's yeast, ginger and cinnamon mixture for preventing or improving obesity, a composition for feed addition.

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