CN116687781A

CN116687781A - Composition for enhancing mitochondrial energy and application thereof

Info

Publication number: CN116687781A
Application number: CN202310830062.4A
Authority: CN
Inventors: 龚德明; 冯川; 席川; 李夏
Original assignee: Guangzhou Liujin Scientific Research Co ltd; Wuhan Twenty Four Bridge Biotechnology Co ltd
Current assignee: Guangzhou Liujin Scientific Research Co ltd; Wuhan Twenty Four Bridge Biotechnology Co ltd
Priority date: 2023-07-07
Filing date: 2023-07-07
Publication date: 2023-09-05

Abstract

The invention provides a composition for enhancing mitochondrial energy and application thereof. The composition for enhancing mitochondrial energy has reasonable compatibility of the components, has a synergistic effect, can provide substances and energy for biochemical processes, can improve the self-oxidation resistance of skin, maintain cell homeostasis, promote collagen synthesis and obviously improve skin aging.

Description

Composition for enhancing mitochondrial energy and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a composition for enhancing mitochondrial energy and application thereof.

Background

Aging is an inevitable natural law in the life process, and is generally considered to be the result of the combined action of various factors including exogenous environmental stimulus, endogenous gene attenuation and the like. The main expression of endogenous gene attenuation is that with the progressive increase of AGEs, collagen forms intermolecular crosslinking, so that not only the elasticity and permeability of skin are reduced, and the diffusion performance of nutrients and wastes is weakened, but also the solubility of collagen is reduced, so that collagen is difficult to hydrolyze by collagenase, the elasticity of skin is reduced, wrinkles are difficult to smooth and deepen continuously, and the aging process of skin is accelerated, so that skin care products with metabolism built on skin surface cells are increasingly favored by people.

CN108245450a discloses a complex containing extract of pomegranate peel and its application. The composite containing the pomegranate peel extract comprises the pomegranate peel extract and glacier water, wherein the ratio of the pomegranate peel extract to the glacier water is 2-100 g/L. The compound disclosed by the compound has the capability of scavenging free radicals and improving autophagy capability of cells, achieves the effects of enhancing the antioxidant capability of skin and scavenging skin metabolic wastes so as to delay aging and whiten skin, and can be used as an efficacy additive to be added into cosmetics.

CN113413398A discloses a fish egg extract and its application. The fresh water roe extract, the stock solution of the sea water roe extract and different components provided by the fresh water roe extract have DPPH free radical scavenging activity, which indicates that the roe extract has a certain degree of in vitro antioxidation. The roe extract can inhibit proliferation of breast cancer cells MCF7, induce apoptosis of MCF7 cells, such as morphological change of apoptosis, positive expression of Annexin V/PI, etc.; the production of reactive oxygen species and superoxide within MCF7 cells and mitochondrial depolarization were seen 24h after fish egg extract treatment of the cells.

However, the above products do not take into account barrier repair and immune enhancement of aging fragile skin, and do not achieve an ideal health state. Based on this, it is necessary to develop a skin care product with multiple functions to meet the requirements of modern people.

Disclosure of Invention

In view of the shortcomings of the prior art, it is an object of the present invention to provide a composition for enhancing mitochondrial energy and its use.

In order to achieve the aim of the invention, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a composition for enhancing mitochondrial energy comprising adenosine, sodium mannose phosphate, and mannose.

The adenosine in the composition provided by the invention is an important intermediate for synthesizing Adenosine Triphosphate (ATP), and ATP can improve the metabolic capacity of an organism and slow down aging; the sodium mannose phosphate is a key to initiate lysosomal autophagy, which can be used to promote differentiation of human fat-derived stem cells; the mannose and the adenosine and the mannose sodium phosphate are matched to promote the functional components to penetrate through skin barriers to enter deep skin tissues, increase the functional components to enter mitochondria, further enhance the energy of the mitochondria, improve the autophagy capacity of cells, promote the synthesis of collagen and elastin, and have synergistic interaction, so that substances and energy can be provided for biochemical processes, the self antioxidant capacity of skin can be improved, the cell homeostasis is maintained, the synthesis of collagen is promoted, and the skin aging condition is obviously improved.

Preferably, the mass ratio of the adenosine to the sodium mannose phosphate to the mannose is (3-5): 1-3): 1-2.

Preferably, the composition for enhancing mitochondrial energy further comprises creatine and/or yeast extract.

Preferably, the composition for enhancing mitochondrial energy comprises adenosine, sodium mannose phosphate, mannose, creatine and yeast extract.

As a preferable technical scheme of the invention, the creatine can store and transfer energy for skin cells, is an important source of skin energy, and can stimulate the biological activity of the skin cells in the transformation of DNA synthesis bodies of the skin cells and the repair of DNA, so as to continue skin aging; the yeast extract contains various small molecular amino acids and polysaccharides, has the advantages of rich nutrition, small molecular weight, easy absorption, comprehensive efficacy and the like, can specifically support the self protection of skin, has various effects of delaying skin aging, moisturizing, improving skin quality, tightening, removing wrinkles and the like, and can further improve the effects of enhancing cell energy and delaying skin aging by adding creatine and the yeast extract.

Preferably, the mass ratio of adenosine, sodium mannose phosphate, mannose, creatine and yeast extract is (3-5): 1-3): 1-2): 3-5): 0.1-1.

Wherein the first "3-5" may be 3.2, 3.4, 3.5, 3.7, 3.9, 4, 4.2, 4.5, 4.7, 4.9, etc.;

"1-3" may be 1.2, 1.4, 1.5, 1.6, 1.8, 2, 2.2, 2.4, 2.5, 2.6, 2.8, etc.;

"1-2" may be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, etc.;

the second "3-5" may be 3.2, 3.4, 3.5, 3.7, 3.9, 4, 4.2, 4.5, 4.7, 4.9, etc.;

"0.1-1" may be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, etc.

Specific point values in the numerical ranges are selectable, and will not be described in detail here.

In a second aspect, the present invention provides the use of a composition for enhancing mitochondrial energy according to the first aspect in cosmetics.

Preferably, the cosmetic comprises a cream, lotion, emulsion, face cream or mask.

Preferably, the composition for enhancing mitochondrial energy is 1-15% by mass in the cosmetic, for example, may be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, etc., and specific values in the above numerical ranges are selected, and will not be described in detail herein.

In a third aspect, the present invention provides a skin-lightening anti-wrinkle essence cream comprising a mitochondrial energy enhancing composition as described in the first aspect, a humectant, a thickener, a skin conditioner, an antioxidant, an emulsifier, a skin feel modifier and deionized water.

The gel Yan Kangzhou essence cream disclosed by the invention can provide more nutrition and energy for skin cells through the combination of the composition for enhancing mitochondrial energy and components such as a humectant, an antioxidant and a skin conditioner, so that the cream has a better effect of delaying skin aging and can also have a better effect of resisting saccharification, deeply moisturizing skin and tightening skin.

Preferably, the gel Yan Kangzhou essence cream comprises, by weight, 1-15 parts of the composition for enhancing mitochondrial energy according to the first aspect, 1-20 parts of a humectant, 0.1-1 part of a thickener, 5-10 parts of a skin conditioner, 0.1-1 part of an antioxidant, 1-10 parts of an emulsifier, 1-10 parts of a skin feel modifier and 1-90 parts of deionized water.

The composition for enhancing mitochondrial energy in the gel Yan Kangzhou essence cream of the invention can be added in an amount of 2 parts, 4 parts, 5 parts, 6 parts, 8 parts, 10 parts, 12 parts or 14 parts, etc.;

the humectant can be added in an amount of 1 part, 3 parts, 5 parts, 7 parts, 9 parts, 10 parts, 12 parts, 15 parts, 17 parts, 19 parts, or the like;

the thickener may be added in an amount of 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, or the like;

the skin conditioning agent may be added in an amount of 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, or the like;

the antioxidant may be added in an amount of 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, or 0.9 parts, etc.;

the addition amount of the emulsifier can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

the addition amount of the skin feel modifier can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

the deionized water may be added in an amount of 5 parts, 10 parts, 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, 90 parts, or the like.

Preferably, the humectant comprises any one or a combination of at least two of glycerin, butylene glycol, panthenol, hyaluronic acid, sodium hyaluronate, dipropylene glycol or tremella polysaccharide.

Preferably, the humectant is a combination of butylene glycol, dipropylene glycol, hyaluronic acid, sodium hyaluronate, and tremella polysaccharide.

In the invention, when the humectant is the combination of butanediol, dipropylene glycol, hyaluronic acid, sodium hyaluronate and tremella polysaccharide, the moisturizing effect of the essence cream can be further improved, and the butanediol, dipropylene glycol, hyaluronic acid, sodium hyaluronate and tremella polysaccharide also have a certain synergistic effect on the effects.

Preferably, the thickener comprises any one or a combination of at least two of acrylic acid (esters) and C10-30 alkanol acrylate cross-linked polymers, cetyl alcohol or stearyl alcohol.

Preferably, the antioxidant comprises any one or a combination of at least two of retinol palmitate, p-hydroxyacetophenone or tocopheryl acetate.

Preferably, the emulsifier comprises any one or a combination of at least two of hydrogenated lecithin, phytosterols, C14-22 alcohols/C12-20 alkyl glucosides or ammonium acryloyldimethyl taurate/VP copolymer.

Preferably, the skin feel modifier comprises any one or a combination of at least two of sodium polyacryl dimethyl taurate, polyisobutylene, PEG-7 trimethylol propyl coco ether, cetyl ethyl hexanoate, diisostearyl malate, polydimethylsiloxane, rice (ORYZA SATIVA) germ oil, aluminum starch octenyl succinate, squalane, or jojoba seed oil.

Preferably, the skin conditioning agent comprises any one or a combination of at least two of soluble collagen, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, dipotassium glycyrrhizinate, nicotinamide, beta-glucan, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, yeast lysate, citrus (CITRUS RETICULATA) pericarp extract, chlorella (CHLORELLA VULGARIS)/Bai Yushan bean (LUPINUS ALBUS) protein fermentation product, hydrolyzed royal jelly protein, tetradecylamino Ding Xianxie amino amidobutyric acid urea trifluoroacetate, soluble collagen, saffron extract or sweet orange extract.

Preferably, the skin conditioning agent is a combination of niacinamide, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, soluble collagen and tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate.

In the invention, the skin conditioner is the combination of nicotinamide, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, soluble collagen and tetradecylamino Ding Xianxie amino carbamide butyrate urea trifluoroacetate, so that the antioxidant and anti-saccharification effects of the essence cream can be further enhanced.

Preferably, the gel Yan Kangzhou essence cream further comprises any one or a combination of at least two of a pH regulator, a preservative or a fragrance.

In a fourth aspect, the present invention provides a method for preparing the essence cream of gel Yan Kangzhou according to the third aspect, which comprises the following steps:

(1) Adding deionized water, a humectant and a thickener into a water phase pot, stirring, heating and preserving heat; adding the emulsifier, the skin feel regulator and the antioxidant into an oil phase pot, stirring, heating and preserving heat;

(2) After the heat preservation is finished, pumping water and an oil pot into an emulsifying pot, homogenizing, stirring and cooling under vacuum, adding the composition for enhancing mitochondrial energy and the skin conditioning agent according to the first aspect, and uniformly mixing to obtain the product.

Preferably, the temperature of the incubation in step (1) is 80-85 ℃ (e.g. 81 ℃, 82 ℃, 83 ℃, 84 ℃ or 85 ℃ and the like) and the time of the incubation is 10-20min (11 min, 13min, 15min, 17min or 19min and the like) respectively.

Preferably, the temperature of the homogenization in the step (2) is 60-70 ℃ (for example, 61 ℃, 62 ℃, 63 ℃, 64 ℃, 65 ℃, 66 ℃, 67 ℃, 68 ℃ or 69 ℃ and the like), the time of the homogenization is 5-10min (for example, 6min, 7min, 8min, 9min or 10min and the like), and the rotation speed of the homogenization is 500-1000rpm (for example, 600rpm, 700rpm, 800rpm, 900rpm or 1000rpm and the like).

Preferably, the temperature of the step (2) is reduced to 30-40deg.C (such as 31deg.C, 32deg.C, 33deg.C, 34 deg.C, 35deg.C, 36deg.C, 37deg.C, 38deg.C or 39deg.C).

Preferably, step (2) further comprises adding any one or a combination of at least two of a pH adjuster, a fragrance, or a preservative.

Compared with the prior art, the invention has the following beneficial effects:

(1) The composition for enhancing mitochondrial energy has reasonable compatibility of the components, has a synergistic effect, can provide substances and energy for biochemical processes, can improve the self-oxidation resistance of skin, maintain cell homeostasis, promote collagen synthesis and obviously improve skin aging.

(2) The gel Yan Kangzhou essence cream disclosed by the invention can provide more nutrition and energy for skin cells through the combination of the composition for enhancing mitochondrial energy and components such as a humectant, an antioxidant and a skin conditioner, so that the cream has a better effect of delaying skin aging and can also have a better effect of resisting saccharification, deeply moisturizing skin and tightening skin.

Detailed Description

In order to further describe the technical means adopted by the present invention and the effects thereof, the following describes the technical scheme of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

The adenosine in the embodiment of the invention is purchased from Nanjing Seebeck biotechnology Co., ltd;

the mannose sodium phosphate is purchased from Beijing Yan Zhishan technology Co., ltd;

the mannose is purchased from Beijing yangshan science and technology Co;

the creatine was purchased from AlzChem Trostberg GmbH;

the yeast extract was purchased from Angel Yeast Co.

The remaining materials can be used as long as they are purchased from a regular dealer.

The adenosine, sodium mannose phosphate, mannose, creatine and yeast extract, which are described below, are all calculated by the actual content of the functional components contained in the commercial raw materials, and any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH regulators, antibacterial agents, antioxidants and impurities within the allowable range may be optionally contained in the commercial raw materials.

Example 1

This example provides a composition for enhancing mitochondrial energy comprising 2 parts adenosine, 1 part sodium mannose phosphate, 0.75 part mannose, 2 parts creatine and 0.25 part yeast extract.

Example 2

This example provides a composition for enhancing mitochondrial energy comprising 2.5 parts adenosine, 0.5 parts sodium mannose phosphate, 1 part mannose, 1.5 parts creatine and 0.5 parts yeast extract.

Example 3

This example provides a composition for enhancing mitochondrial energy comprising 1.5 parts adenosine, 1.5 parts sodium mannose phosphate, 0.5 parts mannose, 2.5 parts creatine and 0.2 parts yeast extract.

Example 4

This example provides a composition for enhancing mitochondrial energy that differs from example 1 only in that creatine is not included, its reduced amount is added to the yeast extract, and the remaining components and amounts added are unchanged.

Example 5

This example provides a composition for enhancing mitochondrial energy that differs from example 1 only in that the yeast extract is not included, its reduced amount is added to creatine, and the remaining components and amounts added are unchanged.

Example 6

This example provides a composition for enhancing mitochondrial energy comprising 3 parts adenosine, 0.3 parts sodium mannose phosphate, 1.1 parts mannose, 1 part creatine and 0.6 parts yeast extract.

Example 7

This example provides a composition for enhancing mitochondrial energy comprising 1 part adenosine, 1.6 parts sodium mannose phosphate, 0.4 part mannose, 3 parts creatine, and 0.04 parts yeast extract.

Comparative example 1

This comparative example provides a composition for enhancing mitochondrial energy which differs from example 1 only in that adenosine is not included, its reduced amount is divided into sodium mannose phosphate and mannose in a part ratio, and the remaining components and added amounts are unchanged.

Comparative example 2

This comparative example provides a mitochondrial energy enhancing composition that differs from example 1 only in that sodium mannose phosphate is not included, the reduced amount is apportioned in parts to adenosine and mannose, and the remaining components and amounts added are unchanged.

Comparative example 3

This comparative example provides a mitochondrial energy enhancing composition that differs from example 1 only in that mannose is not included, the reduced amount of which is apportioned in parts to adenosine and sodium mannose phosphate, and the remaining components and amounts of addition are unchanged.

Application example 1

The application example is used for preparing the face-coagulation anti-wrinkle essence cream, and the formula is as follows:

the preparation method comprises the following steps:

(1) Adding deionized water, humectant, skin conditioner and thickener into water phase pot, stirring, heating to 85deg.C, and maintaining for 45min; adding the emulsifier, skin feel regulator and antioxidant into the oil phase pot, stirring and heating to 80 ℃ and preserving heat for 45min;

(2) After the heat preservation is finished, pumping water and an oil pot into an emulsifying pot, homogenizing at 65 ℃ and 800rpm for 20min, cooling to 35 ℃, adding the composition for enhancing mitochondrial energy as described in the embodiment 1, and uniformly mixing to obtain the mitochondrial energy-enhancing composition.

Application examples 2 to 7

The application example provides six kinds of facial-setting anti-wrinkle essence cream, the formula of the cream is different from that of application example 1 only in that the mitochondrial energy-enhancing composition of example 1 is replaced by the mitochondrial energy-enhancing compositions of examples 2-7 with the same additive amount, and other conditions are kept unchanged, and the preparation method refers to application example 1.

Application example 8

The application example provides a facial-setting essence cream, the formula of which is different from that of application example 1 only in that the humectant does not contain butanediol, the decrement of the cream is distributed into dipropylene glycol, hyaluronic acid, sodium hyaluronate and tremella polysaccharide according to the part ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 9

The application example provides a facial-setting essence cream, and the formula of the facial-setting essence cream is different from that of application example 1 only in that the humectant does not contain dipropylene glycol, the reduced amount of the moisturizing agent is distributed into butanediol, hyaluronic acid, sodium hyaluronate and tremella polysaccharide according to the part ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 10

The application example provides a facial-setting essence cream, and the formula of the facial-setting essence cream is different from that of application example 1 only in that the humectant does not contain hyaluronic acid, the reduced amount of the moisturizing agent is distributed into butanediol, dipropylene glycol, acetylated sodium hyaluronate and tremella polysaccharide according to the part ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 11

The application example provides a facial-setting essence cream, and the formula of the facial-setting essence cream is different from that of application example 1 only in that the humectant does not contain sodium hyaluronate, the reduced amount of the moisturizing agent is distributed into butanediol, dipropylene glycol, hyaluronic acid and tremella polysaccharide according to the part ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 12

The application example provides a facial-setting essence cream, and the formula of the cream is different from that of application example 1 only in that the humectant does not contain tremella polysaccharide, the reduced amount of the cream is distributed into butanediol, dipropylene glycol, hyaluronic acid and acetylated sodium hyaluronate according to the part ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 13

The application example provides a skin-congealing anti-wrinkle essence cream, the formula of which is different from that of application example 1 only in that the skin conditioner does not contain nicotinamide, and the reduced amount of the skin conditioner is distributed into lactobacillus/rice fermentation products, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, soluble collagen and tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate in parts ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 14

The application example provides a skin-congealing anti-wrinkle essence cream, the formula of which is different from that of application example 1 only in that the skin conditioner does not contain lactobacillus/rice fermentation products, and the reduction amount of the skin conditioner is distributed into nicotinamide, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, soluble collagen and tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate in parts ratio, and the other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 15

The application example provides a skin-setting anti-wrinkle essence cream, the formula of which is different from that of application example 1 only in that the skin conditioner does not contain dipalmitoyl hydroxyproline, and the reduction amount of the skin conditioner is distributed into nicotinamide, lactobacillus/rice fermentation product, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, soluble collagen and tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate in a part ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 16

The application example provides a skin-setting anti-wrinkle essence cream, the formula of which is different from that of application example 1 only in that the skin conditioner does not contain hydroxypropyl tetrahydropyran triol, and the reduction amount of the skin conditioner is distributed into nicotinamide, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, palmitoyl tripeptide-5, soluble collagen and tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate in parts ratio, and the other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 17

The application example provides a skin-setting anti-wrinkle essence cream, the formula of which is different from that of application example 1 only in that the skin conditioner does not contain palmitoyl tripeptide-5, and the reduction amount of the skin conditioner is distributed into nicotinamide, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, soluble collagen and tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate in parts ratio, and the other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 18

The application example provides a skin-setting anti-wrinkle essence cream, the formula of which is different from that of application example 1 only in that the skin conditioner does not contain soluble collagen, and the reduced amount of the skin conditioner is distributed into nicotinamide, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5 and tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate in parts ratio, and other components and contents are kept unchanged. The preparation method is described in application example 1.

Application example 19

The application example provides a skin-congealing anti-wrinkle essence cream, the formula of which is different from that of application example 1 only in that the skin conditioner does not contain tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate, and the reduced amount of the skin conditioner is distributed into nicotinamide, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5 and soluble collagen in parts by weight, and the other components and contents are kept unchanged. The preparation method is described in application example 1.

Comparative application examples 1 to 3

Comparative application examples 1 to 3 provided three types of cream for keeping youthful looks and reducing wrinkles, the formulation of which is different from that of application example 1 only in that the composition for enhancing mitochondrial energy of example 1 was replaced with the same amount of the composition for enhancing mitochondrial energy of comparative examples 1 to 3, respectively, and the other ingredients and contents were kept unchanged. The preparation method is described in application example 1.

Comparative application example 4

The present comparative application provides a cream whose formulation differs from application 1 only in that it does not contain the mitochondrial energy enhancing composition of example 1, the other ingredients and amounts remaining unchanged. The preparation method is described in application example 1.

Test example 1

Promoting elastin production experiments

Experiments for promoting elastin formation were performed on the gel Yan Kangzhou essence cream provided in application examples 1 to 7 and the essence cream provided in comparative application examples 1 to 4, as follows:

(1) Human fibroblasts were inoculated on 96-well plates at a density of 20000 cells per well, added with a medium, cultured in an incubator at 37℃for 24 hours, after the cells were attached, replaced with a new medium, and each test sample was added (control sample final concentration was 50 mg/mL) separately, 20mmol/L of PBS solution was used as a blank, and then cultured in an incubator at 37℃for 2 days.

(2) After the completion of the culture, the cells were eluted by using a cell lysate and sonicated. The content of elastin is detected by using a Biocolor elastin detection kit: treating a sample with an elastin precipitant, centrifuging to remove supernatant, adding dye and reaction liquid into the precipitate to react to form elastin-dye complex, centrifuging and washing with PBS solution for three times to remove unbound dye, adding a dye release agent to release the dye in the elastin-dye complex, measuring absorbance of the sample at 513nm by using a spectrophotometer, and calculating the expression level of elastin (the expression level of a blank group is 1) according to an absorbance-dye concentration standard curve;

the specific test results are shown in table 1 below:

TABLE 1

Test sample	Expression level of elastin	Test sample	Expression level of elastin
				Application example 1	5.9	Application example 7	5.0
Application example 2	5.6	Comparative application example 1	3.5
				Application example 3	5.8	Comparative application example 2	2.7
Application example 4	4.5	Comparative application example 3	3.8
				Application example 5	4.3	Comparative application example 4	2.0
Application example 6	5.2

From the test data in table 1, it is shown that the expression level of elastin of the gel Yan Kangzhou essence cream provided in the application examples 1 to 3 of the present invention is 5.5 or more, while the expression level of elastin of the essence cream of the comparative application example 4 is only 2.0, which indicates that the essence cream of the present invention can stimulate cell growth and increase skin elasticity after adding the composition for enhancing mitochondrial energy, thereby improving skin condition. The expression level of elastin in the essence cream described in comparative application examples 1-3 is only 2.7-3.8, which fully demonstrates that adenosine, sodium mannose phosphate and mannose are matched with each other in the composition for enhancing mitochondrial energy, and the composition has a synergistic effect in promoting elastin expression. From application examples 4 and 5, it is understood that, when either creatine or yeast extract is absent from the composition for enhancing mitochondrial energy, it also has a negative effect on promoting elastin expression by essential creams.

Test example 2 antioxidant and anti-glycation test

(1) Taking 1g of essence cream of application examples 1-19 and comparative application examples 1-4, adding 2mL of absolute ethyl alcohol, adding 2mL of DPPH solution with 60 mu mol/L after complete dissolution, uniformly mixing, standing for 30min, zeroing by the absolute ethyl alcohol, and measuring absorbance at 517nm to be Ai; the same method is to take 2mL absolute ethanol solvent and 2mL DPPH solution to be mixed uniformly, and the absorbance is determined to be Ac; then, 1g of the composition matrix is dissolved, 2mL of absolute ethyl alcohol is added, and 2mL of absolute ethyl alcohol solvent is added to be mixed uniformly, and the absorbance is determined as Aj.

The radical scavenging rate was calculated as follows.

Clearance (%) = [1- (Ai-Aj)/Ac ] ×100%

Wherein Aj reflects the contribution of the sample itself to absorbance; absorbance values after action of Ai sample on DPPH; ac is the absorption of DPPH itself at the measurement wavelength.

The DPPH free radical scavenging rate reflects the antioxidant capacity to a certain extent, and the higher the scavenging rate is, the better the antioxidant degree is, and the better the anti-aging effect is, so the anti-aging effect can be judged through the research of the DPPH free radical scavenging capacity of the composition. The results are shown in table 2 (5 replicates per group of samples, data presented as average).

(2) Anti-glycation effect was tested according to fluorescence of AGEs: the inhibition of AGEs formation by the cream of application examples 1 to 13 and comparative examples 1 to 4 was evaluated using a glucose- β -Lg simulator system. According to fluorescence of AGEs, under the conditions of excitation wavelength of 325nm and emission wavelength of 440nm, using an F-4600 fluorescence spectrometer, measuring fluorescence intensity of fluorescent AGEs after a blank group and a composition test group are added for 7d reaction, and calculating inhibition rate of fluorescent AGEs (wherein each group of test samples is diluted to 5.0wt% by using phosphate buffer solution), wherein the calculation method is as follows:

inhibition (%) = [ (F0-F1)/F0 ] ×100%;

wherein, F0 is the fluorescence intensity of the blank control group; f1—fluorescence intensity of test group;

the specific test results are shown in table 2:

TABLE 2

As shown in Table 2, the gel Yan Kangzhou essence cream prepared by application examples 1-3 has a good DPPH removing effect, the removing rate is more than 86%, the inhibition rate of fluorescent AGEs is more than 91%, and the essence cream disclosed by the invention has the advantages of being matched with each other, synergistic in synergy, good in antioxidation effect, capable of helping cell saccharification reversion and reducing glycosylation reaction. From application examples 13 to 19, it is understood that the combination of the skin conditioning agents in the essence cream of the present invention has an influence on the antioxidant and anti-glycation effects of the essence cream. As is clear from comparative examples application examples 1 to 3, the combination of adenosine, sodium mannose phosphate and mannose in the composition for enhancing mitochondrial energy can significantly enhance the antioxidant and anti-glycation effects of the cream.

Test example 3

Anti-wrinkle effect experiment

Anti-wrinkle effect experiments were performed on the gel Yan Kangzhou essence cream provided in application examples 1 to 7 and the essence cream provided in comparative application examples 1 to 4; the specific test method is as follows:

(1) The experimental object: 110 female volunteers with healthy skin and age of 35-55 years are selected, wherein the proportion of male and female is half, and the women are randomly divided into 11 groups;

(2) Experimental instrument: skin wrinkle tester VL650 from CK company, germany;

(3) The testing method comprises the following steps: measuring the wrinkle depth by adopting a skin wrinkle tester VL650 of German CK company and using silica gel to sleeve the forehead, respectively testing the forehead wrinkles of a tested person, and taking the average value of three test data; the specific use method of the essence cream comprises the steps of cleaning the face at 8 points in the morning, and respectively using 0.5+/-0.05 g of each test sample essence cream on the face after cleaning the face; the face is cleaned at 8 pm, after the face is cleaned, 0.5+/-0.05 g of essence cream of each test sample is respectively used on the face, and the duration of the experimental period is 60 days; finally, the percentage of reduced wrinkle depth after 30 and 60 days of treatment was calculated;

the specific test results are shown in table 3 below:

TABLE 3 Table 3

From the test data in table 3, the reduced percentage of the wrinkle depth of the essence cream provided in application examples 1-3 of the invention reaches more than 23% after 60 days, which fully shows that the components in the essence cream are mutually matched and synergistically enhanced, can effectively resist skin wrinkles and slow down the skin aging process, reduce the generation of static wrinkles, and improve the dynamic wrinkles of the skin by promoting the expression of skin elastin and the proliferation and growth of cells. As is clear from comparative application examples 1 to 3, the composition for enhancing mitochondrial energy had reduced wrinkle-removing effect of essence cream in the absence of any one of adenosine, sodium mannose phosphate and mannose.

Test example 4

Moisture retention performance test:

the gel Yan Kangzhou cream prepared in application examples 1-3 and 8-12 was tested and compared to the skin moisture content of the test subjects before and after use (consistency in use operation), and the test subjects were 80 volunteers, of which women: 56 men, 24, aged 20-40 years, average age 28 years, were randomized into 8 groups of 7 women and 3 men.

The subject cleans the forearms of both hands, sits still for 30min under the set humidity environment, and selects 3X 3cm for each of the left and right forearms of the subject ² The water content of each experimental part was measured with Corneometer CM825 with the left arm as the test area of the test sample and the symmetric area of the right arm as the blank control, repeated 3 times, and an average value was obtained for recording.

The skin hydration rate was calculated as:

the average skin hydration results of the subjects after 2 hours of use of the samples are shown in table 4:

TABLE 4 Table 4

Group of	Skin hydration rate
		Application example 1	44.5％
Application example 2	43.7％
		Application example 3	42.9％
Application example 8	35.6％
		Application example 9	32.4％
Application example 10	36.6％
		Application example 11	38.3％
Application example 12	36.5％

As can be seen from the data in table 4: the gel Yan Kangzhou essence cream has a stronger moisturizing effect; the five components of butanediol, dipropylene glycol, hyaluronic acid, acetylated sodium hyaluronate and tremella polysaccharide in the humectant have a certain synergistic effect on the effects, and the five components are indispensable.

Test example 5: erythrocyte hemolysis experiment

The erythrocyte hemolysis test (Red blood cell haemolysis test, RBC) is one of the alternative methods for the rabbit eye-stimulating test, and the basic principle is to evaluate the damage of chemicals to eye tissue cells by measuring the amount of cellular hemoglobin and the degree of denaturation. RBC experiments can be used to evaluate eye irritation of cosmetics.

Erythrocyte hemolysis experiments were performed on the gel Yan Kangzhou essence cream prepared in application examples 1-19. RBC experiment test method and judgment standard of European alternative method verification center are shown in Table 5, wherein HD ₅₀ At a sample concentration of 50% when erythrocyte hemolysis occurs, DI is the protein denaturation index, and L/D is HD ₅₀ Ratio to DI.

TABLE 5

The results of the irritation of each of the samples to be tested prepared in application examples 1 to 19 are shown in Table 6.

TABLE 6

As can be seen from the sample irritation results in Table 6, the irritation of the gel Yan Kangzhou essence cream prepared in application examples 1-19 of the invention is classified as non-irritating, which indicates that each component in the essence cream of the invention has the characteristics of mildness and non-irritation.

The applicant states that the present invention is illustrated by the above examples as a composition for enhancing mitochondrial energy and its use, but the invention is not limited to, i.e. it is not meant that the invention must be practiced in dependence upon the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.

The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.

In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.

Claims

1. A composition for enhancing mitochondrial energy, wherein the composition for enhancing mitochondrial energy comprises adenosine, sodium mannose phosphate, and mannose.

2. The mitochondrial energy enhancing composition of claim 1 wherein the mass ratio of adenosine, sodium mannose phosphate, and mannose is (3-5): 1-3): 1-2.

3. The mitochondrial energy enhancing composition of claim 1 or 2, wherein the mitochondrial energy enhancing composition further comprises creatine and/or yeast extract;

preferably, the mitochondrial energy enhancing composition comprises adenosine, sodium mannose phosphate, mannose, creatine and yeast extract;

4. Use of a composition for enhancing mitochondrial energy according to any one of claims 1-3 in cosmetics.

5. The use according to claim 4, wherein the cosmetic comprises a cream, lotion, emulsion, face cream or mask;

preferably, the composition for enhancing mitochondrial energy comprises 1-15% by mass of the cosmetic.

6. A facial and anti-wrinkle essence cream comprising the composition for enhancing mitochondrial energy of any one of claims 1 to 3, a humectant, a thickener, a skin conditioner, an antioxidant, an emulsifier, a skin feel modifier, and deionized water.

7. The gel Yan Kangzhou essential cream of claim 6, wherein the gel Yan Kangzhou essential cream comprises, in parts by weight, 1-15 parts of the composition for enhancing mitochondrial energy of any one of claims 1-3, 1-20 parts of a humectant, 0.1-1 part of a thickener, 5-10 parts of a skin conditioner, 0.1-1 part of an antioxidant, 1-10 parts of an emulsifier, 1-10 parts of a skin feel modifier, and 1-90 parts of deionized water.

8. The gel Yan Kangzhou essence cream of claim 6 or 7, wherein the humectant comprises any one or a combination of at least two of glycerin, butylene glycol, panthenol, hyaluronic acid, sodium hyaluronate, dipropylene glycol, or tremella polysaccharide;

preferably, the humectant is a combination of butylene glycol, dipropylene glycol, hyaluronic acid, sodium hyaluronate and tremella polysaccharide;

preferably, the thickener comprises any one or a combination of at least two of acrylic acid (esters) and C10-30 alkanol acrylate cross-linked polymers, cetyl alcohol or stearyl alcohol;

preferably, the antioxidant comprises any one or a combination of at least two of retinol palmitate, p-hydroxyacetophenone or tocopheryl acetate;

preferably, the emulsifier comprises any one or a combination of at least two of hydrogenated lecithin, phytosterols, C14-22 alcohol/C12-20 alkyl glucoside or ammonium acryloyldimethyl taurate/VP copolymer;

preferably, the skin feel modifier comprises any one or a combination of at least two of sodium polyacryl dimethyl taurate, polyisobutylene, PEG-7 trimethylol propyl coco ether, cetyl ethyl hexanoate, diisostearyl malate, polydimethylsiloxane, rice germ oil, aluminum starch octenyl succinate, squalane, or jojoba seed oil;

preferably, the skin conditioning agent comprises any one or a combination of at least two of soluble collagen, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, dipotassium glycyrrhizinate, nicotinamide, beta-glucan, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, yeast lysate, citrus peel extract, chlorella/Bai Yushan soy protein fermentation product, hydrolyzed royal jelly protein, tetradecylamino Ding Xianxie amino amidobutyrate urea trifluoroacetate, soluble collagen, saffron extract or sweet orange extract;

preferably, the skin conditioning agent is a combination of niacinamide, lactobacillus/rice fermentation product, dipalmitoyl hydroxyproline, hydroxypropyl tetrahydropyran triol, palmitoyl tripeptide-5, soluble collagen, tetradecylamino Ding Xianxie amino butyric acid urea trifluoroacetate;

9. A method of preparing a gel Yan Kangzhou essence cream according to any one of claims 6 to 8, comprising:

(2) After the heat preservation is finished, pumping water and an oil pot into an emulsifying pot, homogenizing, stirring and cooling under vacuum, adding the composition for enhancing mitochondrial energy and the skin conditioning agent according to any one of claims 1-3, and uniformly mixing.

10. The method for preparing the essence cream of congee Yan Kangzhou according to claim 9, wherein the temperature of the heat preservation in the step (1) is respectively and independently 80-85 ℃, and the time of the heat preservation is respectively and independently 10-20min;

preferably, the homogenizing temperature in the step (2) is 78-85 ℃, the homogenizing time is 5-10min, the homogenizing rotating speed is 3000-5000rpm, and the stirring speed is 20-30rpm;

preferably, the temperature in the step (2) is reduced to 30-40 ℃;