CN116649340A - Bactericide composition for preventing and treating damping-off of white gourd - Google Patents
Bactericide composition for preventing and treating damping-off of white gourd Download PDFInfo
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- CN116649340A CN116649340A CN202310663069.1A CN202310663069A CN116649340A CN 116649340 A CN116649340 A CN 116649340A CN 202310663069 A CN202310663069 A CN 202310663069A CN 116649340 A CN116649340 A CN 116649340A
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- damping
- white gourd
- preventing
- bactericide composition
- cinnamaldehyde
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- 235000011274 Benincasa cerifera Nutrition 0.000 title claims abstract description 24
- 244000036905 Benincasa cerifera Species 0.000 title claims abstract description 24
- 241000918585 Pythium aphanidermatum Species 0.000 title claims abstract description 20
- 239000000203 mixture Substances 0.000 title claims abstract description 17
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 12
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 12
- DXYYSGDWQCSKKO-UHFFFAOYSA-N 2-methylbenzothiazole Chemical compound C1=CC=C2SC(C)=NC2=C1 DXYYSGDWQCSKKO-UHFFFAOYSA-N 0.000 claims abstract description 24
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 claims abstract description 17
- 229940117916 cinnamic aldehyde Drugs 0.000 claims abstract description 17
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000005794 Hymexazol Substances 0.000 claims abstract description 14
- KGVPNLBXJKTABS-UHFFFAOYSA-N hymexazol Chemical compound CC1=CC(O)=NO1 KGVPNLBXJKTABS-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000013329 compounding Methods 0.000 claims abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 239000000575 pesticide Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 239000000447 pesticide residue Substances 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 description 8
- 241000813090 Rhizoctonia solani Species 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 229940079593 drug Drugs 0.000 description 6
- 239000013043 chemical agent Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 231100000419 toxicity Toxicity 0.000 description 5
- 230000001988 toxicity Effects 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 206010059866 Drug resistance Diseases 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 241000816814 Rhizoctonia solani AG-4 Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 241000239290 Araneae Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 239000005843 Thiram Substances 0.000 description 1
- 229930195482 Validamycin Natural products 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000009916 joint effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- KUAZQDVKQLNFPE-UHFFFAOYSA-N thiram Chemical compound CN(C)C(=S)SSC(=S)N(C)C KUAZQDVKQLNFPE-UHFFFAOYSA-N 0.000 description 1
- 229960002447 thiram Drugs 0.000 description 1
- JARYYMUOCXVXNK-IMTORBKUSA-N validamycin Chemical compound N([C@H]1C[C@@H]([C@H]([C@H](O)[C@H]1O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)CO)[C@H]1C=C(CO)[C@H](O)[C@H](O)[C@H]1O JARYYMUOCXVXNK-IMTORBKUSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating damping-off of white gourd. A bactericide composition for preventing and treating white gourd damping-off is prepared by binary compounding of cinnamaldehyde and hymexazol or 2-methylbenzothiazole. When the cinnamaldehyde and hymexazol or 2-methylbenzothiazole are compounded in the bactericide composition, the co-toxicity coefficients are all larger than 120, and the synergistic effect is realized, so that the control effect on the damping-off of the white gourd can be improved, the pesticide application dosage is reduced, and the pesticide residue and the environmental pollution are reduced.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating damping-off of white gourd.
Background
The white gourd damping off is a white gourd disease caused by a rhizoctonia solani AG-4 hypha fusion group (Rhizoctonia solani K uhn AG-4). The seedling blight of the white gourd mainly occurs at the later stage of seedling cultivation, the seedling raising tray is in a higher temperature condition or in a direct seeding field, the seedling blight is mainly caused by damaging the underground root or the seedling stem base, irregular or nearly elliptic dark spots appear at the affected part at first, the affected part is slightly sunken, the stem is caused to wilt and dry after the affected part is expanded around the stem for one week, and the death of the melon seedlings is caused. The early stage is similar to damping-off, but the damping-off is rapid, and the disease course of the white gourd damping-off is slower, and the disease part has insignificant or significant round lines and light brown spider filamentous mold, namely mycelium or sclerotium of germ, which is different from the damping-off.
The chemical agent is a main means for preventing and treating the damping off of the white gourd, and common chemical agents include validamycin, hymexazol, thiram and the like. However, long-term administration of a single chemical agent tends to cause development and development of drug resistance, and the efficacy of the chemical agent is reduced, so that screening of a compound agent having a synergistic effect is necessary. The inventor finds that when cinnamaldehyde is compounded with hymexazol or 2-methylbenzothiazole through an indoor biological activity test, the co-toxicity coefficient of the cinnamaldehyde and the hymexazol to the damping-off bacteria of the white gourd is greater than 120, and the synergistic effect is shown. No relevant report is currently seen in this regard.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a bactericide composition for preventing and treating white gourd damping-off, which aims to solve the problem of single chemical agent application.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a bactericide composition for preventing and treating white gourd damping-off is prepared by binary compounding of cinnamaldehyde and hymexazol or 2-methylbenzothiazole.
Preferably, the mass ratio of the cinnamaldehyde to the hymexazol is 1-5:30-1.
Preferably, the mass ratio of the cinnamaldehyde to the 2-methylbenzothiazole is 1-20:9-1.
Compared with the prior art, the invention has the following beneficial effects:
(1) When the cinnamaldehyde and hymexazol or 2-methylbenzothiazole are compounded, the co-toxicity coefficients are all larger than 120, and the synergistic effect is shown, so that the control effect on the damping-off of the white gourd can be improved, the pesticide application dosage is reduced, and the pesticide residue and the environmental pollution are reduced.
(2) The bactericide composition can delay the development of drug resistance of pathogenic bacteria and reduce the risk of drug resistance.
Detailed Description
The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: screening of control agents
1. Test strain: the strain DGLK of the white gourd rhizoctonia solani is obtained from a white gourd plant infected with the white gourd rhizoctonia solani, is separated, purified and tiebased in a laboratory, identified as a fusion group (Rhizoctonia solani K ulhn AG-4) of the white gourd rhizoctonia solani AG-4, and is stored on a PDA culture medium.
2. Test agent
98.0% of cinnamaldehyde, 98.0% of hymexazol and 98.0% of 2-methylbenzothiazole are all commercially available.
3. The test method comprises the following steps: reference to NY/T1156.2-2006 section 2 of pesticide indoor bioassay test criteria section 2: test plate method for inhibiting growth of pathogenic fungi hyphae.
4. Test method
(1) Preparing a strain: taking out strain DGLK of Rhizoctonia solani from refrigerator at 4deg.C, inoculating on PDA culture medium, culturing in constant temperature incubator at 28deg.C for 3 days;
(2) Dissolving the raw materials with dimethyl sulfoxide, and diluting to obtain single-dose mother solution; and setting a plurality of groups of proportions, wherein each single-dose mother solution and the proportion mixture are set according to a certain proportion, and each proportion is provided with 5 mass concentration gradients. (3) Mixing the medicines with different concentrations with PDA culture medium, pouring the mixture into a plate to prepare culture medium plates with different concentrations, taking and cutting the cultured DGLK strain by a puncher with the diameter of 7mm, and inoculating the DGLK strain to the PDA plates with medicines with serial concentrations; with the corresponding dimethyl sulfoxide as a control, and a blank control was set, each treatment was repeated 3 times, and all treatments were cultured in a constant temperature incubator at 28 ℃.
5. Recording the result and calculating: when the diameter of the blank control colony grows to about 2/3 of the diameter of the culture dish, measuring the colony diameter by a crisscross method, and calculating the inhibition rate of different treatments on hypha growth. Conversion of hypha growth inhibition rate into several values (y), conversion of the concentration (mg/L) of the drug into a logarithmic value based on 10, and calculation of virulence regression equation of each test drug by DPS software, effective inhibition of medium concentration (EC) 50 ) And correlation coefficients, and co-toxicity coefficients (CTC values) of the blends were calculated according to Sun Yunpei method.
Hypha growth inhibition ratio = [ (control colony diameter-cake diameter) - (treated colony diameter-cake diameter) ]/(control colony diameter-cake diameter) ×100%;
measured virulence index (ATI) = (standard agent EC 50 Reagent EC 50 )×100；
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture;
co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/(theoretical drug susceptibility index (TTI) ] x 100.
Dividing the standard according to the joint action: the co-toxicity coefficient (CTC) is more than or equal to 120, and the synergistic effect is shown; the cotoxicity coefficient (CTC) is less than or equal to 80 and shows antagonism; 80< co-toxicity coefficient (CTC) <120 shows additive effect. The experimental results are shown in tables 1-2.
TABLE 1 indoor biological Activity of cinnamaldehyde and hymexazol on Benincase rhizoctonia solani
As can be seen from Table 1, cinnamaldehyde and hymexazol are mixed according to the ratio of 1-5: when the mass ratio of 30-1 is compounded, the co-toxicity coefficient is more than 120, the synergistic effect is shown, and the control effect on the damping-off of the white gourd can be improved.
TABLE 2 indoor biological Activity of cinnamaldehyde and 2-methylbenzothiazole on Benincasa hispida
As can be seen from Table 2, cinnamaldehyde and 2-methylbenzothiazole were mixed in an amount of 1 to 30: when the mass ratio of 9-1 is compounded, the co-toxicity coefficient is more than 120, the synergistic effect is shown, and the control effect on the damping-off of the white gourd can be improved.
In conclusion, when the cinnamaldehyde and hymexazol or 2-methylbenzothiazole are compounded, the co-toxicity coefficient is more than 120, and the synergistic effect is shown, so that the control effect on the damping-off of the white gourd can be improved, the pesticide application dosage is reduced, and the pesticide residue and the environmental pollution are reduced.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (3)
1. The bactericide composition for preventing and treating the white gourd damping-off is characterized by being prepared by binary compounding of cinnamaldehyde and hymexazol or 2-methylbenzothiazole.
2. The bactericide composition according to claim 1, wherein the mass ratio of cinnamaldehyde to hymexazol is 1 to 5:30-1.
3. The bactericide composition according to claim 1, characterized in that the mass ratio of cinnamaldehyde to 2-methylbenzothiazole is 1-20:9-1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310663069.1A CN116649340A (en) | 2023-06-06 | 2023-06-06 | Bactericide composition for preventing and treating damping-off of white gourd |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310663069.1A CN116649340A (en) | 2023-06-06 | 2023-06-06 | Bactericide composition for preventing and treating damping-off of white gourd |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116649340A true CN116649340A (en) | 2023-08-29 |
Family
ID=87725848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310663069.1A Pending CN116649340A (en) | 2023-06-06 | 2023-06-06 | Bactericide composition for preventing and treating damping-off of white gourd |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116649340A (en) |
-
2023
- 2023-06-06 CN CN202310663069.1A patent/CN116649340A/en active Pending
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