CN116508911A - Biological fertilizer water preparation for aquaculture and preparation method and application thereof - Google Patents
Biological fertilizer water preparation for aquaculture and preparation method and application thereof Download PDFInfo
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- CN116508911A CN116508911A CN202310419554.4A CN202310419554A CN116508911A CN 116508911 A CN116508911 A CN 116508911A CN 202310419554 A CN202310419554 A CN 202310419554A CN 116508911 A CN116508911 A CN 116508911A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 96
- 239000003337 fertilizer Substances 0.000 title claims abstract description 84
- 238000002360 preparation method Methods 0.000 title claims abstract description 55
- 238000009360 aquaculture Methods 0.000 title claims abstract description 24
- 244000144974 aquaculture Species 0.000 title claims abstract description 23
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 38
- 239000011591 potassium Substances 0.000 claims abstract description 38
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 34
- 235000019764 Soybean Meal Nutrition 0.000 claims abstract description 32
- 235000012054 meals Nutrition 0.000 claims abstract description 32
- 239000004455 soybean meal Substances 0.000 claims abstract description 32
- 240000008042 Zea mays Species 0.000 claims abstract description 31
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 31
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 31
- 235000005822 corn Nutrition 0.000 claims abstract description 31
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 28
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 28
- 235000018102 proteins Nutrition 0.000 claims abstract description 27
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 27
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 27
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 25
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 25
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 25
- 241000195628 Chlorophyta Species 0.000 claims abstract description 12
- 108010068370 Glutens Proteins 0.000 claims abstract description 7
- 235000019779 Rapeseed Meal Nutrition 0.000 claims abstract description 7
- 235000021312 gluten Nutrition 0.000 claims abstract description 7
- 239000004456 rapeseed meal Substances 0.000 claims abstract description 7
- 241001247197 Cephalocarida Species 0.000 claims abstract description 5
- 241000239250 Copepoda Species 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims description 47
- 101710089042 Demethyl-4-deoxygadusol synthase Proteins 0.000 claims description 30
- 235000021121 fermented vegetables Nutrition 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 22
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 5
- 235000019750 Crude protein Nutrition 0.000 claims description 5
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 5
- 239000002509 fulvic acid Substances 0.000 claims description 5
- 229940095100 fulvic acid Drugs 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 239000003674 animal food additive Substances 0.000 claims description 2
- 241000206761 Bacillariophyta Species 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 6
- 239000002028 Biomass Substances 0.000 abstract description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 3
- 230000002180 anti-stress Effects 0.000 abstract description 3
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 3
- 239000011574 phosphorus Substances 0.000 abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 239000006041 probiotic Substances 0.000 abstract 1
- 235000018291 probiotics Nutrition 0.000 abstract 1
- 239000013589 supplement Substances 0.000 abstract 1
- 241000195493 Cryptophyta Species 0.000 description 17
- 239000000203 mixture Substances 0.000 description 15
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 13
- 230000000694 effects Effects 0.000 description 13
- 238000012360 testing method Methods 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000009629 microbiological culture Methods 0.000 description 6
- 241000238553 Litopenaeus vannamei Species 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 241000238017 Astacoidea Species 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002686 phosphate fertilizer Substances 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
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Abstract
The invention relates to the technical field of aquaculture, in particular to a biological fertilizer water preparation for aquaculture and a preparation method and application thereof. The invention discloses a biological fertilizer agent for aquaculture, which is mainly prepared from potassium fulvate, fermented soybean meal, distillers dried grains with soluble substances (DDGS), fermented rapeseed meal, corn gluten meal, bacillus subtilis and lactobacillus plantarum. The biological fertilizer water agent can provide protein and carbon sources required by zooplankton, supplement elements such as phosphorus and potassium required by beneficial zooplankton such as green algae, diatom and the like, and the probiotics can reduce the concentration of harmful substances in water and enhance the organism immunity and anti-stress capability of seedlings. The biological fertilizer aqueous agent can obviously improve the density of phytoplankton and zooplankton in the culture water body, and especially can increase the biomass of green algae, diatom, copepod and cladocera.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a biological fertilizer water preparation for aquaculture and a preparation method and application thereof.
Background
Aquaculture occupies an important position in the agricultural economy of China, and pond culture is a main culture mode in aquaculture. The use of the fertilizer-water preparation in pond culture has important effect and directly affects the success and yield of culture due to the limitation of a plurality of factors. The fertilizer-water preparation can not only increase the variety and quantity of phytoplankton in the water body, improve the dissolved oxygen in the water body, adjust the transparency of the water body, avoid direct sunlight and control the growth and propagation of moss; the bait can also provide high-quality bait for the aquatic animals, strengthen the physique of the aquatic animals in the seedling stage and improve the anti-stress capability; the fertilizer-water preparation can also reduce the concentration of harmful substances in the water body and promote the circulation of the nutrient substances in the water body, thereby keeping the water quality stable.
At present, aquaculture fertilizer aqueous solutions are various, and commonly used inorganic fertilizers and organic fertilizers are used. The inorganic fertilizer mainly comprises nitrogen phosphate fertilizer, potash fertilizer, calcium fertilizer and the like, and has the advantages of quick effect of fertilizer water, short maintenance time, single nutrition, easy absorption by algae, mass propagation of certain algae caused by the easy absorption of algae, quick change of nutrient components of water body and easy algae conversion or algae pouring. The organic fertilizer mainly comprises manure, green manure, mixed compost and the like, has rich organic matter content and lasting fertilizer efficiency, but has slow fertilizer water speed, consumes a large amount of oxygen, has low effective nitrogen content, lacks phosphorus and potassium and other elements, is easy to produce various toxic and harmful substances, and is not beneficial to the growth of aquatic organisms. Therefore, the development of a multifunctional novel fertilizer-water preparation which can meet the requirements of high yield, high efficiency, green and safety of the current aquaculture is a problem to be solved urgently in the current aquaculture industry.
Disclosure of Invention
In order to solve the technical problems, the invention provides a biological fertilizer agent applied to aquaculture, which comprises the following specific scheme:
a biological fertilizer agent for aquaculture is prepared from (by mass) potassium fulvate 65-75%, fermented soybean meal 10-14%, DDGS 5-15%, fermented rapeseed meal 3-5%, corn protein powder 3-6%, bacillus subtilis CGMCCNo.10055 bacterial powder 0.1% and lactobacillus plantarum CGMCCNo.9358 bacterial powder 0.01%.
Further, the biological fertilizer water agent is prepared from 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 0.1% of bacillus subtilis CGMCCNo.10055 bacterial powder and 0.01% of lactobacillus plantarum CGMCCNo.9358 bacterial powder.
Further, the viable count of the bacillus subtilis CGMCC No.10055 bacterial powder is not less than 1 multiplied by 10 10 CFU/g，The viable count of the lactobacillus plantarum CGMCC No.9358 bacterial powder is not less than 1 multiplied by 10 10 CFU/g。
Further, the potassium fulvate is biochemical potassium fulvate, contains 60% of organic matters, 45% of fulvic acid and 24% of crude protein.
Further, the protein content of the fermented soybean meal is 50%, the protein content of the DDGS is 28%, the protein content of the fermented vegetable meal is 40%, and the protein content of the corn protein powder is 60%.
The invention also provides a preparation method of the biological fertilizer aqueous agent, which comprises the following steps:
(1) Pulverizing fermented soybean meal, DDGS, fermented vegetable meal and corn protein powder, sieving with 60 mesh sieve.
(2) Weighing potassium fulvate, fermented soybean meal, DDGS, fermented vegetable meal, corn protein powder, bacillus subtilis CGMCCNo.10055 bacterial powder and lactobacillus plantarum CGMCCNo.9358 bacterial powder according to mass percentage, and uniformly mixing to obtain the feed additive.
The invention also provides application of the biological fertilizer water agent in improving the phytoplankton density and zooplankton density in the aquiculture water body. The phytoplankton is green algae and/or diatom. The zooplankton is copepoda and/or cladocera.
Compared with the prior art, the invention has the beneficial effects that:
the biological fertilizer agent for aquaculture provided by the invention is used for selecting a biological organic fertilizer as a main nutrition source according to the growth environment requirements of cultured animals and providing protein and carbon sources required by zooplankton; and supplementing elements such as phosphorus and potassium required by beneficial phytoplankton such as green algae and diatom, and strengthening water quality; the bacillus subtilis and lactobacillus plantarum contained in the water body can decompose organic matters in the water body, reduce the concentration of harmful substances in the water body, provide nutrition for plankton, and enhance the organism immunity and anti-stress capability of seedlings.
Experiments prove that the biological fertilizer aqueous agent can obviously improve the density of phytoplankton and zooplankton in the culture water body, and especially can increase the biomass of green algae, diatom, copepod and cladocera.
The biological fertilizer water agent has simple preparation process, wide application range and obvious proliferation effect on plankton, can reduce the concentration of harmful substances in water, has good low-temperature fertilizer water effect and lasting fertilizer effect.
Detailed Description
To further embody the features and advantages of the present invention, the following detailed description is provided in connection with the results of specific embodiments. The invention is not limited to the following embodiments.
Example 1
The biological fertilizer water agent for aquaculture consists of the following components in percentage by mass: 65% of potassium fulvate, 12% of fermented soybean meal, 15% of DDGS, 4% of fermented vegetable meal, 3.89% of corn protein powder, 141010.1% of bacillus subtilis DBN and 20.01% of lactobacillus plantarum RS.
Wherein, the potassium fulvate is biochemical potassium fulvate, contains 60% of organic matters, 45% of fulvic acid and 24% of crude protein; the protein content of the fermented soybean meal is 50%, the protein content of DDGS is 28%, the protein content of the fermented vegetable meal is 40%, and the protein content of the corn protein powder is 60%;
the collection number of the bacillus subtilis DBN14101 is CGMCC No.10055, and the bacillus subtilis is collected in the China general microbiological culture Collection center (China general microbiological culture Collection center) with the collection address of 11 months and 25 days in 2014: the source of the microbiological institute of China academy of sciences of national academy of sciences of China No. 3, north Chen West Lu 1, chaoyang area of Beijing is farm wastewater, the preparation is bacterial powder, and the number of viable bacteria is required to reach 1 multiplied by 10 10 CFU/g。
The lactobacillus plantarum (Lactobacillus plantarum) RS-2 has a preservation number of CGMCC No.9358 and is preserved in the China general microbiological culture Collection center (China general microbiological culture Collection center) for 6 months and 18 days in 2014, with a preservation address: the source of the microbiological institute of China academy of sciences of national academy of sciences of China No. 3, north Chen Silu 1, chaoyang area of Beijing city is intestinal tracts of cultured animals, the preparation is bacterial powder, and the number of viable bacteria is required to reach 1 multiplied by 10 10 CFU/g。
The preparation method comprises the following steps:
(1) Pulverizing fermented soybean meal, DDGS, fermented vegetable meal and corn protein powder, sieving with 60 mesh sieve.
(2) Weighing the following components in percentage by mass of the potassium fulvate and the raw materials in the step (1): 65% of potassium fulvate, 12% of fermented soybean meal, 15% of DDGS, 4% of fermented vegetable meal, 3.89% of corn protein powder, 141010.1% of bacillus subtilis DBN and 20.01% of lactobacillus plantarum RS.
(3) And (3) fully mixing and uniformly stirring the components weighed in the step (2) in a mixer to finally obtain a mixture.
(4) And (3) drying, sealing and preserving the mixture obtained in the step (3) to obtain the biological fertilizer water agent for aquaculture.
Example 2
The biological fertilizer water agent for aquaculture consists of the following components in percentage by mass: 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 141010.1% of bacillus subtilis DBN and 20.01% of lactobacillus plantarum RS.
Wherein, the potassium fulvate is biochemical potassium fulvate, contains 60% of organic matters, 45% of fulvic acid and 24% of crude protein; the protein content of the fermented soybean meal is 50%, the protein content of DDGS is 28%, the protein content of the fermented vegetable meal is 40%, and the protein content of the corn protein powder is 60%; the collection number of the bacillus subtilis DBN14101 is CGMCC No.10055, and the bacillus subtilis is collected in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms in 11 and 25 of 2014, the source is wastewater of a culturing farm, the preparation is bacterial powder, and the number of the viable bacteria is required to reach 1 multiplied by 10 10 CFU/g. The lactobacillus plantarum RS-2 has a preservation number of CGMCC No.9358, is preserved in the common microorganism center of China Committee for culture Collection of microorganisms in the 6 th and 18 th of 2014, and is derived from intestinal tracts of cultured animals, and the preparation is bacterial powder, wherein the number of viable bacteria is required to reach 1 multiplied by 10 10 CFU/g。
The preparation method comprises the following steps:
(1) Pulverizing fermented soybean meal, DDGS, fermented vegetable meal and corn protein powder, sieving with 60 mesh sieve.
(2) Weighing the following components in percentage by mass of the potassium fulvate and the raw materials in the step (1): 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 141010.1% of bacillus subtilis DBN and 20.01% of lactobacillus plantarum RS.
(3) And (3) fully mixing and uniformly stirring the components weighed in the step (2) in a mixer to finally obtain a mixture.
(4) And (3) drying, sealing and preserving the mixture obtained in the step (3) to obtain the biological fertilizer water agent for aquaculture.
Example 3
The biological fertilizer water agent for aquaculture consists of the following components in percentage by mass: 75% of potassium fulvate, 10% of fermented soybean meal, 5% of DDGS, 5% of fermented vegetable meal, 4.89% of corn gluten meal, 141010.1% of bacillus subtilis DBN and 20.01% of lactobacillus plantarum RS.
Wherein, the potassium fulvate is biochemical potassium fulvate, contains 60% of organic matters, 45% of fulvic acid and 24% of crude protein; the protein content of the fermented soybean meal is 50%, the protein content of DDGS is 28%, the protein content of the fermented vegetable meal is 40%, and the protein content of the corn protein powder is 60%; the collection number of the bacillus subtilis DBN14101 is CGMCC No.10055, and the bacillus subtilis is collected in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms in 11 and 25 of 2014, the source is wastewater of a culturing farm, the preparation is bacterial powder, and the number of the viable bacteria is required to reach 1 multiplied by 10 10 CFU/g. The lactobacillus plantarum RS-2 has a preservation number of CGMCC No.9358, is preserved in the common microorganism center of China Committee for culture Collection of microorganisms in the 6 th and 18 th of 2014, and is derived from intestinal tracts of cultured animals, and the preparation is bacterial powder, wherein the number of viable bacteria is required to reach 1 multiplied by 10 10 CFU/g。
The preparation method comprises the following steps:
(1) Pulverizing fermented soybean meal, DDGS, fermented vegetable meal and corn protein powder, sieving with 60 mesh sieve.
(2) Weighing the following components in percentage by mass of the potassium fulvate and the raw materials in the step (1): 75% of potassium fulvate, 10% of fermented soybean meal, 5% of DDGS, 5% of fermented vegetable meal, 4.89% of corn gluten meal, 141010.1% of bacillus subtilis DBN and 20.01% of lactobacillus plantarum RS.
(3) And (3) fully mixing and uniformly stirring the components weighed in the step (2) in a mixer to finally obtain a mixture.
(4) And (3) drying, sealing and preserving the mixture obtained in the step (3) to obtain the biological fertilizer water agent for aquaculture.
Test example 1
Test grouping:
test 1 to 3 groups: the biological fertilizer agent prepared in the embodiment 1 to 3 of the invention;
comparative example 1: the biological fertilizer water agent is prepared from 70% of potassium fulvate, 16% of fermented soybean meal, 10% of fermented rapeseed meal and 4% of corn gluten meal according to parts by weight, and the preparation method is the same as that of example 1;
comparative example 2: the biological fertilizer water agent is prepared from 70 weight parts of potassium fulvate, 16 weight parts of fermented soybean meal, 10 weight parts of DDGS and 4 weight parts of corn gluten meal by the same method as in example 1;
comparative example 3: the biological fertilizer water agent is prepared from 70% of potassium fulvate, 16% of fermented soybean meal, 5% of fermented rapeseed meal, 5% of DDGS and 4% of corn protein powder according to parts by weight, and the preparation method is the same as that of example 1;
comparative example 4: the biological fertilizer water agent is prepared from 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 141010.1% of bacillus subtilis DBN and 0.01% of saccharomyces cerevisiae LMY in parts by weight, and the preparation method is the same as that of example 1;
comparative example 5: the biological fertilizer water agent is prepared from 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 20.01% of lactobacillus plantarum RS and 0.1% of saccharomyces cerevisiae LMY in parts by weight, and the preparation method is the same as that of example 1;
comparative example 6: the biological fertilizer water agent is prepared from 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 0.1% of bacillus subtilis 5#and 20.01% of lactobacillus plantarum RS according to parts by weight, and the preparation method is the same as that of example 1;
comparative example 7: the biological fertilizer water agent is prepared from 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 141010.1% of bacillus subtilis DBN and 0.01% of lactobacillus plantarum FJR in parts by weight, and the preparation method is the same as that of example 1;
comparative example 8: the biological fertilizer water agent is prepared from 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.79% of corn protein powder, 141010.1% of bacillus subtilis DBN, 20.01% of lactobacillus plantarum RS and 0.1% of saccharomyces cerevisiae LMY in parts by weight, and the preparation method is the same as that of example 1;
the test method comprises the following steps: the biological fertilizer water preparation prepared in the examples 1-3 and the biological fertilizer water preparation prepared in the comparative examples 1-8 are added into 100ml of water, and are fully and uniformly shaken to prepare a product mother solution (the product concentration is 5 g/L) for the cultivation experiment of chlorella. The experiments were carried out in transparent cultivation boxes (18X 11 cm), 1L of aerated water was added to each box, and 1ml of supplementary nutrient solution and 10ml of chlorella liquid (counted at the time of experiment) were added. 1ml of mother liquor of the experimental product is added into the experimental group, uniformly mixed and marked. The concentration of the supplementary nutrient solution in the final experimental culture box is 1.5g/m 3 Water, product concentration 5g/m 3 Water and natural illumination. The number of chlorella was counted under a microscope with a hemocytometer every day, and the observation was continued for 7 days, and d0 was recorded on the first day of the start of the experiment, followed by d1, d2, d3, d4, d5 and d6 in this order. Each experimental group was set with 4 replicates. In the test process, the strain preparations are all powder, and the number of effective viable bacteria reaches 1X 10 10 CFU/g。
Test results:
TABLE 1 Chlorella quantity variation (10 5 Personal/ml)
As can be seen from the experimental results in Table 1, the amount of the chlorella in examples 1-3 is obviously increased, and the amount of the chlorella can be maintained at a higher level, which indicates that the biological fertilizer water preparation in the examples can promote the growth and the reproduction of the chlorella. Wherein the chlorella population in example 2 increased at the fastest rate and d2 increasedAs long as 20.8 x 10 5 The number of chlorella per ml can be kept at a high level from d2 to d6. After the application of the biological fertilizer aqueous agent in comparative example 3, the increase in the number of chlorella was smoother than that of two groups of comparative examples 1 and 2. The biological fertilizer aqueous solution prepared by the composite addition of the fermented vegetable meal and the DDGS has better growth effect on chlorella than the biological fertilizer aqueous solution prepared by the single-component addition when other components are unchanged and the addition content is the same. From the experimental results of example 2 and comparative examples 4 to 8, it can be seen that the number of chlorella was similar in trend. After the application of the bio-fertilizer agent in example 2, the increase effect of the number of chlorella was better than that of comparative examples 4, 5, 6, 7 and 8. The biological fertilizer aqueous solution prepared by adding the bacillus subtilis DBN14101 and the lactobacillus plantarum RS-2 compound microecological preparation is superior to the biological fertilizer aqueous solution prepared by adding other compound microecological preparations in the growth effect of chlorella.
Test example 2
The effect comparison is carried out by using the biological fertilizer water preparation in the example 2 and a biological fertilizer water preparation product A which is commonly used in the market, and the influence of the biological fertilizer water preparation and the product A on the density of phytoplankton in the culture water body in practical application is compared. Wherein, the components of the product A mainly comprise: potassium fulvate, fermented soybean meal, fermented rapeseed meal, amino acids, bacillus subtilis 0.1% (1×10) 8 CFU/g), enterococcus faecalis, mineral elements, and the like.
The method is characterized in that a water fertilization effect test of a high-temperature cultivation season is carried out in a Penaeus vannamei Boone cultivation pond in Jiangsu province, east county, the biological fertilizer water preparation is applied to an experimental group, the biological fertilizer water preparation is applied to a control group, the application amount of the biological fertilizer water preparation is 2 kg/mu, and the number change of phytoplankton in the water body is monitored continuously for 5 days after the biological fertilizer water preparation is applied to the control group. The phytoplankton species composition and variation are shown in tables 2 and 3.
TABLE 2 Phytoplankton Density variation after application of control group Water-based fertiliser formulation
As can be seen from table 2, after the control group of bio-fertilizer water preparation was used, dominant algae in the penaeus vannamei boone pond was changed from blue algae to green algae. The density increase rate of the green algae is 689.99%, the biological density increase rate of the diatom is 538.49%, the biological density increase rate of the blue algae is 121.48%, and the total phytoplankton density is 337.99% during the experiment, which indicates that the use of the biological fertilizer agent of the control group can improve the phytoplankton density in the water body, and the fertilizer water effect is obvious.
TABLE 3 Phytoplankton Density variation after application of the Experimental group of Water-based fertiliser formulations
As can be seen from Table 3, the amount of blue algae in the Penaeus vannamei Boone pond is dominant before the biological fertilizer water preparation of the present invention is used. After the biological fertilizer water agent product is used, green algae is dominant, the ratio of the green algae is about 55%, the density increase rate is 1607.79%, the total phytoplankton density is increased by 829.23%, and the biological fertilizer water agent product is higher than that of a control group. The biological fertilizer water preparation provided by the invention can obviously improve the phytoplankton density in the water body, has more obvious advantage on the growth of green algae, effectively improves the quantity of beneficial algae in the penaeus vannamei boone culture water body, improves the algae composition, stabilizes the algae phase, greatly shortens the water-fertilizing time, and provides high-quality biological baits for the penaeus vannamei boone.
Test example 3
The biological fertilizer water preparation of the embodiment 2 of the invention and the related biological fertilizer water preparation product A in the market are selected and used for testing the fertilizer water effect in the crayfish winter culture pond. The biological fertilizer water preparation of the invention is applied as an experimental group, and the product A is applied as a control group. The crayfish culture pond in the Datong lake area in Yiyang city of Hunan province is selected, the area is about 40 mu, the average water depth is 1m, and the use amount of the biological fertilizer water preparation is 40kg. The number of plankton changes in the water body were monitored for 5 consecutive days. The plankton species composition and biomass growth rate in the pond before and after application are shown in tables 4 and 5.
TABLE 4 plankton Density Change after application of control group Water-fertilizer formulation
As can be seen from Table 4, after the control group of the water aqua, the phytoplankton green algae in the pond accounts for about 15.73%, the diatom accounts for about 71.91%, and the blue algae accounts for about 12.36%. The biological density of all three algae was reduced and the total phytoplankton density was reduced by 31.72%, indicating that the control bio-fertilizer water formulation in this example failed to increase the phytoplankton density in the pond. Most zooplankton in the pond are radial feet and branch angles, the increase rate of the branch angles is 386.88%, the number of other zooplankton is reduced by more than 50%, and the total zooplankton density is reduced by 15.25%. The comparison group biological fertilizer water preparation cannot improve the density of zooplankton in the water body in the embodiment.
TABLE 5 plankton Density Change after application of Experimental group of Water formulations
As can be seen from Table 5, the total phytoplankton density in the water body increased by 97.74% after the use of the bio-fertilizer water preparation of the invention, wherein the diatom ratio having an important role in the low temperature cultivation of crayfish is increased from the initial 32.41% to 77.87%, and the biological density is increased by 375%. The invention can effectively increase the quantity of beneficial algae in the water body, improve the algae composition and stabilize the algae phase. The zooplankton in the pond is mostly copepods and cladocera, the proportion is increased to 44.5% and 46%, and the total zooplankton density is increased by 42.86% obviously. The biological fertilizer water preparation provided by the invention can improve the natural biological bait density in the water body, and provides more high-quality protein sources for the crayfish in the seedling stage.
Compared with the control group, the biological fertilizer water preparation has better proliferation effect on phytoplankton and zooplankton.
The foregoing is a part of the examples of the present invention and is not intended to limit the invention, so that the technical matter according to the invention can be equally changed or improved without departing from the technical content of the invention, and the invention is covered by the patent coverage.
Claims (9)
1. The biological fertilizer agent for aquaculture is characterized by being prepared from, by mass, 65-75% of potassium fulvate, 10-14% of fermented soybean meal, 5-15% of DDGS, 3-5% of fermented rapeseed meal, 2.89-5.89% of corn gluten meal, 0.1% of bacillus subtilis CGMCC No.10055 bacterial powder and 0.01% of lactobacillus plantarum CGMCC No.9358 bacterial powder.
2. The biological fertilizer water aqua of claim 1, which is characterized by being prepared from 70% of potassium fulvate, 14% of fermented soybean meal, 10% of DDGS, 3% of fermented vegetable meal, 2.89% of corn protein powder, 0.1% of bacillus subtilis CGMCCNo.10055 bacterial powder and 0.01% of lactobacillus plantarum CGMCCNo.9358 bacterial powder.
3. The biological fertilizer water agent according to claim 1 or 2, characterized in that the viable count of the bacillus subtilis CGMCC No.10055 bacterial powder is not less than 1 x 10 10 CFU/g, the viable count of the lactobacillus plantarum CGMCC No.9358 bacterial powder is not less than 1 multiplied by 10 10 CFU/g。
4. The biological fertilizer water aqua according to claim 1 or 2, wherein the potassium fulvate is a biochemical potassium fulvate, and comprises 60% of organic matters, 45% of fulvic acid and 24% of crude proteins.
5. The aqueous bio-fertilizer according to claim 1 or 2, wherein the protein content of the fermented soybean meal is 50%, the protein content of the DDGS is 28%, the protein content of the fermented rapeseed meal is 40%, and the protein content of the corn gluten meal is 60%.
6. The preparation method of the biological fertilizer water agent as claimed in claim 1 or 2, which is characterized by comprising the following steps:
(1) Pulverizing fermented soybean meal, DDGS, fermented vegetable meal and corn protein powder, sieving with 60 mesh sieve.
(2) Weighing potassium fulvate, fermented soybean meal, DDGS, fermented vegetable meal, corn protein powder, bacillus subtilis CGMCCNo.10055 bacterial powder and lactobacillus plantarum CGMCCNo.9358 bacterial powder according to mass percentage, and uniformly mixing to obtain the feed additive.
7. Use of the aqueous bio-fertilizer according to claim 1 or 2 for increasing phytoplankton density and zooplankton density in an aquaculture water body.
8. The use according to claim 7, wherein the phytoplankton is green algae and/or diatoms.
9. The use according to claim 7, wherein the zooplankton is a copepoda and/or a cladocera.
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