CN116270968A - Antibacterial liquid and preparation method thereof - Google Patents

Antibacterial liquid and preparation method thereof Download PDF

Info

Publication number
CN116270968A
CN116270968A CN202310278104.8A CN202310278104A CN116270968A CN 116270968 A CN116270968 A CN 116270968A CN 202310278104 A CN202310278104 A CN 202310278104A CN 116270968 A CN116270968 A CN 116270968A
Authority
CN
China
Prior art keywords
parts
essential oil
carboxymethyl chitosan
carboxymethyl
antibacterial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202310278104.8A
Other languages
Chinese (zh)
Inventor
刘美霞
胡振东
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Fengho Brand Management Co ltd
Original Assignee
Qingdao Fengho Brand Management Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Fengho Brand Management Co ltd filed Critical Qingdao Fengho Brand Management Co ltd
Priority to CN202310278104.8A priority Critical patent/CN116270968A/en
Publication of CN116270968A publication Critical patent/CN116270968A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/716—Glucans
    • A61K31/717—Celluloses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/716—Glucans
    • A61K31/722—Chitin, chitosan
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/728—Hyaluronic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/234—Cnidium (snowparsley)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/282—Artemisia, e.g. wormwood or sagebrush
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/286—Carthamus (distaff thistle)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/489—Sophora, e.g. necklacepod or mamani
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/533—Leonurus (motherwort)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/756—Phellodendron, e.g. corktree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/14—Peptides containing saccharide radicals; Derivatives thereof, e.g. bleomycin, phleomycin, muramylpeptides or vancomycin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Dermatology (AREA)
  • Reproductive Health (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The application relates to the field of gynecological gel, and in particular discloses a bacteriostatic liquid and a preparation method thereof, wherein the bacteriostatic liquid is prepared from the following raw materials in parts by weight: 0.1-0.3 part of bioactive peptide; 0.05-0.2 parts of marine glycoprotein; 2-4 parts of active polysaccharide; 15-20 parts of plant extract; 0.5-1.5 parts of sodium hyaluronate; 0.1-0.25 part of plant essential oil; 0.8-1.5 parts of lactic acid; 1-2 parts of carboxymethyl cellulose; 1-1.5 parts of glycerol; 1-2 parts of pH regulator; 0.5-1.5 parts of emulsifying agent; the antibacterial liquid is an aqueous solution containing carboxymethyl chitosan, the number average molecular weight of the carboxymethyl chitosan is 1000-20000, purified water is added to 100 parts, and the concentration of the carboxymethyl chitosan aqueous solution is not less than 0.1wt%; the carboxymethyl chitosan is O-carboxymethyl chitosan, and the nourishing antibacterial gel can be used for gynecological bacteriostasis and has the advantages of good antibacterial effect and skin moistening.

Description

Antibacterial liquid and preparation method thereof
Technical Field
The application relates to the field of gynecological gel, in particular to a bacteriostatic liquid and a preparation method thereof.
Background
With the development of economy, the improvement of the living standard and the acceleration of the living rhythm of people, the population with the female body in sub-health inflammation is increasing. At present, the conventional preparations commonly used for the local treatment of gynecological inflammation mainly comprise: water, tablet, suppository, ointment, gel, etc.
The Chinese patent with publication number of CN105997847A discloses a bacteriostatic gel, wherein each liter of bacteriostatic gel consists of the following raw materials: chlorhexidine acetate 0.1-1.0 g, mung bean fermentation liquor 0.5-1.5 ml, alcohol 10-20ml, polyquaternium-371.0-4.0 g, 1.3-butanediol 2.0-8.0 g, trehalose 0.2-1.8 g, chinese medicinal extract 0.2-0.8 g, and purified water.
Aiming at the related technology, the inventor considers that the antibacterial gel mainly adopts synthetic medicines to perform antibacterial, and is difficult to maintain the skin of the private part while performing antibacterial.
Disclosure of Invention
In order to improve gel antibacterial rate and maintain skin at the same time, the application provides antibacterial liquid and a preparation method thereof.
In a first aspect, the present application provides a bacteriostatic liquid, which adopts the following technical scheme:
the antibacterial liquid is prepared from the following raw materials in parts by weight: 0.1-0.3 part of bioactive peptide;
0.05-0.2 parts of marine glycoprotein;
2-4 parts of active polysaccharide;
15-20 parts of plant extract;
0.5-1.5 parts of sodium hyaluronate;
0.1-0.25 part of plant essential oil;
0.8-1.5 parts of lactic acid;
1-2 parts of carboxymethyl cellulose;
1-1.5 parts of glycerol;
1-2 parts of pH regulator;
0.5-1.5 parts of emulsifying agent;
purified water is added to 100 parts;
the plant extract is prepared from the following raw materials in parts by weight:
8-10 parts of kuh-seng;
6-8 parts of phellodendron;
2-4 parts of fructus cnidii;
4-6 parts of red sage root;
4-6 parts of safflower;
4-8 parts of motherwort;
1-2 parts of mugwort leaf;
the antibacterial liquid is an aqueous solution containing carboxymethyl chitosan, and the number average molecular weight of the carboxymethyl chitosan is 1000-20000.
By adopting the technical scheme, as the plant extract, the plant essential oil and the lactic acid are compounded, the plant extract contains active ingredients for inhibiting candida albicans, escherichia coli and staphylococcus aureus, the plant essential oil, also called volatile oil, essential oil and aromatic oil, is a volatile and simple compound with smaller molecular weight and certain activity which exists in an aromatic plant body, can be distilled along with water vapor, and can be mutually immiscible with water, besides being applied as a flavoring raw material, the plant essential oil has various functional effects such as antibacterial effect, insecticidal effect and antioxidation effect, promotes tissue cell regeneration effect, and the lactic acid has antimicrobial property, can inhibit the growth of microorganisms, and the applicant finds that the antibacterial rate can be obviously improved by synergy when the plant extract, the tea tree essential oil and the lactic acid are compounded for use.
The bioactive peptide is a multifunctional compound derived from protein, has different biological activities, is generally composed of several to tens of amino acids, has various amino acid sequences and various structures, has better effects on the aspect of shallow wrinkle depth, can enhance the elasticity and the protein activity of private cells, and can maintain the cell renewal capacity, so that the vagina is more viable, and the vagina is more compact; the marine glycoprotein is obtained from marine psychrotolerant pseudomonas antarctica NF3 strain by biotechnology, and has effects of improving skin adhesion, promoting skin reconstruction, and improving skin firmness and elasticity. The active polysaccharide can promote the synthesis of collagen by fibroblasts, promote wound healing, repair damaged skin, smooth wrinkles and improve elasticity. When the bioactive peptide, the marine glycoprotein and the active polysaccharide are compounded for use, the bioactive peptide, the marine glycoprotein and the active polysaccharide can be synergistic, and the elasticity of the skin can be obviously enhanced.
Optionally, the active polysaccharide is composed of schizophyllan and ginkgo polysaccharide;
the concentration of the carboxymethyl chitosan aqueous solution is not less than 0.1wt%;
the carboxymethyl chitosan is O-carboxymethyl chitosan;
the preparation method of the carboxymethyl chitosan comprises the steps of degrading carboxymethyl chitosan raw materials with higher number average molecular weight through hydrogen peroxide, wherein the number average molecular weight of the carboxymethyl chitosan in the carboxymethyl chitosan raw materials with higher number average molecular weight is 30-70;
the substitution degree of carboxymethyl in the carboxymethyl chitosan raw material with higher number average molecular weight is 0.8-1.3;
by adopting the technical scheme, the schizophyllum commune is a large fungus with higher nutritive value and medicinal value, and schizophyllum commune polysaccharide extracted from the schizophyllum commune is provided with physiological activities of inhibiting tumors, improving organism immunity, delaying aging, enhancing skin elasticity and the like; ginkgo polysaccharide can also enhance skin elasticity. The schizophyllan and ginkgo polysaccharide are compounded for use, so that the skin elasticity increasing rate can be improved.
Optionally, the weight part ratio of the schizophyllan to the ginkgo polysaccharide is 1: (2-4);
by adopting the technical scheme, schizophyllan and ginkgo polysaccharide can be synergistically enhanced under a specific proportion, and the elasticity increasing rate is obviously improved.
Optionally, the plant essential oil is composed of calendula essential oil and chamomile essential oil;
the degradation process of hydrogen peroxide is as follows: adding hydrogen peroxide into carboxymethyl chitosan with higher number average molecular weight under stirring to perform degradation reaction, adding alkali in the degradation reaction process to enable the pH of a reaction system to be 8.0-8.5, and adding sodium sulfite to stop the reaction until the viscosity of the reaction system is 80-120 cp;
the preparation method of the carboxymethyl chitosan comprises the steps of carrying out acid precipitation washing on the carboxymethyl chitosan after hydrogen peroxide degradation;
the acid precipitation washing is to adjust the pH of the carboxymethyl chitosan material degraded by hydrogen peroxide to 6-7, add ethanol until light yellow crystals are separated out, repeatedly wash with ethanol, spin-dry and dry to obtain carboxymethyl chitosan;
by adopting the technical scheme, the calendula essential oil is petal extract of calendula of the asteraceae, and has the effect of resisting aging. The calendula extract contains lutein and zeaxanthin, has anti-inflammatory and antibacterial effects on flowers and leaves, and particularly has good effects on staphylococcus and streptococcus. The chamomile essential oil can relieve inflammation and pain symptoms of patients. The cyclic ethers, flavones, total volatile oil and the like in the chamomile have different inhibition effects on fungal growth; flos Matricariae Chamomillae is rich in flavonoid active ingredient, and has antioxidant, anti-vascular proliferation, antiinflammatory, antiallergic and antiviral effects. When the calendula essential oil and the chamomile essential oil are used together, the antibacterial rate can be improved.
Optionally, the weight part ratio of the calendula essential oil to the chamomile essential oil is 1: (1-3);
by adopting the technical scheme, calendula essential oil and chamomile essential oil can be synergistically enhanced under a specific proportion, and the antibacterial rate is obviously improved.
Optionally, the antibacterial liquid is prepared from the following raw materials in parts by weight:
0.1 part of bioactive peptide;
0.2 parts of marine glycoprotein;
3 parts of active polysaccharide;
18 parts of a plant extract;
1 part of sodium hyaluronate;
0.2 parts of plant essential oil;
1 part of lactic acid;
1.5 parts of carboxymethyl cellulose;
glycerol 1.2 parts;
1.5 parts of pH regulator;
1 part of an emulsifying agent;
purified water is added to 100 parts;
the plant extract is prepared from the following raw materials in parts by weight:
9 parts of kuh-seng;
7 parts of phellodendron;
3 parts of fructus cnidii;
5 parts of red sage root;
4 parts of safflower;
6 parts of motherwort;
2 parts of mugwort leaf.
By adopting the technical scheme, the gel has better antibacterial effect and skin elasticity increasing effect when the gel is mixed.
In a second aspect, the present application provides a preparation method of a bacteriostatic liquid, which adopts the following technical scheme:
a preparation method of the antibacterial liquid comprises the following steps:
step one, adding a proper amount of purified water into lactic acid, and dissolving to obtain a lactic acid solution;
pulverizing radix Sophorae Flavescentis, cortex Phellodendri, fructus Cnidii, saviae Miltiorrhizae radix, carthami flos, herba Leonuri and folium Artemisiae Argyi into coarse powder, and percolating to obtain plant extract;
dissolving carboxymethyl cellulose, bioactive peptide, active polysaccharide and sodium hyaluronate in a proper amount of purified water, standing for 10-12 hours to fully swell the carboxymethyl cellulose, and uniformly stirring to obtain a gel matrix;
step four, diluting the plant essential oil and the glycerol with a proper amount of ethanol, adding an emulsifying agent, and uniformly stirring to obtain an essential oil mixture;
and fifthly, adding the mixture of the lactic acid solution, the plant extract and the essential oil into the gel matrix, uniformly stirring, adding purified water to a sufficient amount, slowly adding the pH regulator to the pH of 4-6 under the stirring state, and uniformly stirring to obtain the gel.
By adopting the technical scheme, the obtained gel has hydrophilicity, macromolecular active polysaccharide and bioactive peptide in the gel have water absorbability, swell after water absorbability to form a 3D framework, and active components such as small molecular active components and moisturizing components of traditional Chinese medicines are wrapped in the gel, so that the gel has the functions of resisting bacteria and trichomonas, relieving itching, activating cell activity, promoting tissue repair and the like of female private parts, and the private parts can be healthier, tighter and more moist.
Optionally, the second step specifically includes: pulverizing radix Sophorae Flavescentis, cortex Phellodendri, fructus Cnidii, saviae Miltiorrhizae radix, carthami flos, herba Leonuri, and folium Artemisiae Argyi into coarse powder, extracting by percolation method, using 55% ethanol as solvent, adding ethanol 2-3 times of the coarse powder in the first time, soaking for 5-7 days, slowly percolating, adding ethanol 3-4 times of the coarse powder in the second time, soaking for 3-5 days, slowly percolating, squeezing residues, mixing 2 percolates, mixing uniformly, decolorizing with active carbon, recovering ethanol, concentrating to relative density of 1.08-1.10 at 60deg.C, and collecting plant extract.
By adopting the technical scheme, the method can fully extract the effective components in the plants.
In summary, the present application has the following beneficial effects:
because the application adopts the plant extract, tea tree essential oil and lactic acid to compound and use, the synergistic effect can be achieved, and the antibacterial rate is obviously improved; when the bioactive peptide, the marine glycoprotein and the active polysaccharide are compounded for use, the bioactive peptide, the marine glycoprotein and the active polysaccharide can be synergistic, and the elasticity of the skin can be obviously enhanced.
2. In the application, schizophyllan and ginkgo polysaccharide are preferably compounded for use, so that the skin elasticity increasing rate can be improved, and the schizophyllan and ginkgo polysaccharide can be synergistically increased under a specific proportion, so that the elasticity increasing rate is remarkably improved.
3. In the application, calendula essential oil and chamomile essential oil are preferably matched for use, so that the antibacterial rate can be improved. And the calendula essential oil and the chamomile essential oil can synergistically increase the antibacterial rate under a specific proportion.
Detailed Description
The present application is described in further detail below with reference to examples.
Examples
Example 1
An antibacterial liquid is prepared from the raw materials shown in table 1, and the preparation method of the antibacterial liquid comprises the following steps: step one, adding 10g of purified water into lactic acid, and dissolving to obtain a lactic acid solution;
pulverizing radix Sophorae Flavescentis, cortex Phellodendri, fructus Cnidii, saviae Miltiorrhizae radix, carthami flos, herba Leonuri and folium Artemisiae Argyi into coarse powder, extracting by percolation method, using 55% ethanol as solvent, soaking for 5 days with ethanol 2 times of the coarse powder for the first time, slowly percolating, adding ethanol 3 times of the coarse powder for the second time, soaking for 3 days for the second time, slowly percolating, squeezing residues, mixing 2 times of percolate, mixing uniformly, decolorizing with active carbon, recovering and standing for more than 24 hr, recovering ethanol, and concentrating to relative density of 1.08-1.10 at 60deg.C to obtain plant extract;
dissolving carboxymethyl cellulose, bioactive peptide, marine glycoprotein, active polysaccharide and sodium hyaluronate in 20g of purified water, standing for 10 hours to fully swell the carboxymethyl cellulose, and uniformly stirring to obtain a gel matrix;
step four, adding 2g of ethanol into the plant essential oil and the glycerol for dilution, adding an emulsifying agent, and uniformly stirring to obtain an essential oil mixture;
and fifthly, adding the mixture of the lactic acid solution, the plant extract and the essential oil into the gel matrix, uniformly stirring, adding purified water to a sufficient amount, slowly adding the pH regulator to the pH of 4 under the stirring state, and uniformly stirring to obtain the gel.
Wherein the plant extract is prepared from the following raw materials in parts by weight: 8g of kuh-seng; 6g of phellodendron; 2g of fructus cnidii; 4g of red sage root; 4g of safflower; 4g of motherwort; mugwort leaf 1g.
The active polysaccharide is angelica polysaccharide, the plant essential oil is tea tree essential oil, the tea tree essential oil is purchased from Guangzhou Wuzhi Kogyo Biotechnology Co., ltd., the pH regulator is sodium hydroxide, the emulsifier is Tween 80, the bioactive peptide is acetyl hexapeptide-8, and the marine glycoprotein is purchased from Spanish Li Putai.
Example 2
A bacteriostatic liquid was prepared from the raw materials shown in Table 1, which was different from example 1. Wherein the plant extract is prepared from the following raw materials in parts by weight: radix Sophorae Flavescentis 9g; 7g of phellodendron; 3g of fructus cnidii; 5g of red sage root; safflower 5g; 6g of motherwort; 1.5g of mugwort leaf;
the antibacterial liquid is an aqueous solution containing carboxymethyl chitosan, and the number average molecular weight of the carboxymethyl chitosan is 1000-20000.
Example 3
A bacteriostatic liquid was prepared from the raw materials shown in Table 1, which was different from example 1. Wherein the plant extract is prepared from the following raw materials in parts by weight: 10g of kuh-seng; cortex phellodendri 8g; 4g of fructus cnidii; 6g of red sage root; 6g of safflower; motherwort herb
8g; mugwort leaf (folium Artemisiae Argyi) 2g.
Example 4
A bacteriostatic liquid was prepared from the raw materials shown in Table 1, which was different from example 1.
TABLE 1 raw materials for examples 1-4 and weights (g)
Figure SMS_1
Example 5
An antibacterial liquid is different from example 1 in that the active polysaccharide consists of 2g schizophyllan and 2g ginkgo polysaccharide.
Example 6
An antibacterial liquid is different from example 1 in that the active polysaccharide consists of 0.57g schizophyllan and 3.43g ginkgo polysaccharide.
Example 7
An antibacterial liquid is different from example 1 in that the active polysaccharide consists of 1.33g schizophyllan and 2.67g ginkgo polysaccharide.
Example 8
An antibacterial liquid is different from example 1 in that the active polysaccharide consists of 1g schizophyllan and 3g ginkgo polysaccharide.
Example 9
An antibacterial liquid is different from example 1 in that the active polysaccharide consists of 0.8g schizophyllan and 3.2g apricot polysaccharide.
Example 10
An antibacterial liquid is different from example 1 in that the active polysaccharide is replaced with schizophyllan.
Example 11
An antibacterial liquid is different from example 1 in that the active polysaccharide is replaced by ginkgo polysaccharide.
Example 12
A bacteriostatic liquid is different from example 1 in that the plant essential oil consists of 0.134g of calendula essential oil and
066g of chamomile essential oil.
Example 13
A bacteriostatic liquid was different from example 1 in that the plant essential oil consisted of 0.033 g calendula essential oil and 0.167g chamomile essential oil.
Example 14
A bacteriostatic liquid was different from example 1 in that the plant essential oil consisted of 0.1g calendula essential oil and 0.1g chamomile essential oil.
Example 15
A bacteriostatic liquid was different from example 1 in that the plant essential oil consisted of 0.066 g calendula essential oil and 0.134g chamomile essential oil.
Example 16
A bacteriostatic liquid was different from example 1 in that the plant essential oil consisted of 0.05g calendula essential oil and 0.15g chamomile essential oil.
Example 17
A bacteriostatic liquid differs from example 1 in that the plant essential oil is calendula essential oil.
Example 18
An antibacterial liquid is different from example 1 in that the plant essential oil is chamomile essential oil.
Example 19
The difference from example 1 is that the preparation method of the antibacterial liquid comprises the following steps:
step one, adding 10g of purified water into lactic acid, and dissolving to obtain a lactic acid solution;
pulverizing radix Sophorae Flavescentis, cortex Phellodendri, fructus Cnidii, saviae Miltiorrhizae radix, carthami flos, herba Leonuri and folium Artemisiae Argyi into coarse powder, extracting by percolation method, using 55% ethanol as solvent, soaking for 7 days with ethanol 3 times of the coarse powder for the first time, slowly percolating, adding ethanol 4 times of the coarse powder for the second time, soaking for 5 days for the second time, slowly percolating, squeezing residues, mixing 2 times of percolate, mixing uniformly, decolorizing with active carbon, recovering and standing for more than 24 hr, recovering ethanol, and concentrating to relative density of 1.08-1.10 at 60deg.C to obtain plant extract;
dissolving carboxymethyl cellulose, bioactive peptide, marine glycoprotein, active polysaccharide and sodium hyaluronate in 20g of purified water, standing for 12 hours to fully swell the carboxymethyl cellulose, and uniformly stirring to obtain a gel matrix;
step four, adding 2g of ethanol into the plant essential oil and the glycerol for dilution, adding an emulsifying agent, and uniformly stirring to obtain an essential oil mixture; step five, adding the mixture of lactic acid solution, plant extract and essential oil into the gel matrix, stirring uniformly, and adding purified water
And (3) slowly adding the pH regulator to the pH of 6 under the stirring state until the water is enough, and uniformly stirring to obtain the gel.
The concentration of the carboxymethyl chitosan aqueous solution is not less than 0.1wt%;
the carboxymethyl chitosan is O-carboxymethyl chitosan;
the preparation method of the carboxymethyl chitosan comprises the steps of degrading carboxymethyl chitosan raw materials with higher number average molecular weight through hydrogen peroxide, wherein the number average molecular weight of the carboxymethyl chitosan in the carboxymethyl chitosan raw materials with higher number average molecular weight is 30-70 ten thousand;
the carboxymethyl substitution degree in the carboxymethyl chitosan raw material with higher number average molecular weight is 0.8-1.3.
Comparative example
Comparative example 1
A bacteriostatic liquid is different from example 1 in that sodium hyaluronate, bioactive peptides, marine glycoproteins, active polysaccharides, and glycerol are not added.
Comparative example 2
A bacteriostatic liquid was different from comparative example 1 in that 0.1g of bioactive peptide was added.
Comparative example 3
A bacteriostatic liquid was different from comparative example 1 in that 0.05g of marine glycoprotein was added.
Comparative example 4
A bacteriostatic liquid was different from comparative example 1 in that 4g of active polysaccharide was added.
Comparative example 5
A bacteriostatic liquid is different from example 1 in that sodium hyaluronate and glycerin are not added.
Comparative example 6
A bacteriostatic liquid is different from example 1 in that bioactive peptide, active polysaccharide, plant extract, plant essential oil and lactic acid are not added.
Comparative example 7
A bacteriostatic liquid was different from comparative example 6 in that 15g of a plant extract was added.
Comparative example 8
A bacteriostatic liquid was different from comparative example 6 in that 0.1g of plant essential oil was added.
Comparative example 9
A bacteriostatic liquid was different from comparative example 6 in that 1.5g of lactic acid was added.
Comparative example 10
A bacteriostatic liquid was different from comparative example 6 in that 15g of a plant extract, 0.1g of a plant essential oil, and 1.5g of lactic acid were added.
Performance test
Test method
(1) The gels of examples 1-4, examples 12-19 and comparative examples 6-10 were tested for their antibacterial activity against E.coli (8099), candida albicans (ATCC 10231), staphylococcus aureus (ATCC 6538) for 10min according to the test conditions in GB15979-2002, appendix C4 of the sanitary Standard for Disposable sanitary articles and the 2002 edition of the technical Specification for Disinfection, test
Repeating the steps three times, taking an average value, and taking a bacteriostasis test as a carrier test, wherein the test temperature is 20+/-1 ℃.
(2) Collecting 16 volunteers, allowing a tester to clean the inner side of a handle, naturally airing, standing in a room with constant temperature and humidity for 15min, marking the inner side of the tester's arm, performing blank test by using a skin elasticity tester, and recording 4 skin elasticity data; the gels of examples 1 to 11 and comparative examples 1 to 5 were applied to 16 volunteers, respectively, once a day, in the same amount each time, and after 5, 10 and 15 days of application, the skin elasticity was measured, and the rate of increase in skin elasticity was calculated.
Table 2 results of bacteriostasis test
Figure SMS_2
TABLE 3 skin elasticity test results
Figure SMS_3
It can be seen from the combination of examples 1-4, examples 12-19 and comparative examples 6-10 and the combination of table 2 that the antibacterial gel of the application has a strong antibacterial effect by adopting the combination of the plant extract, the plant essential oil and the lactic acid in examples 1-3 and having the antibacterial rate of more than 80% on three pathogenic bacteria. When no antibacterial component is added in comparative example 6, the antibacterial rate is 0%, the antibacterial rate is about 72% after the plant extract is added in comparative example 7 on the basis of comparative example 6, the antibacterial rate is about 65% after the tea tree essential oil is added in comparative example 8 on the basis of comparative example 6, the antibacterial rate is about 62% after the lactic acid is added in comparative example 9, which indicates that the plant extract, the tea tree essential oil and the lactic acid all have certain antibacterial effects, and the antibacterial rate is about 79% after the plant extract, the tea tree essential oil and the lactic acid are simultaneously added in comparative example 6, which indicates that the plant extract, the tea tree essential oil and the lactic acid can synergistically increase when being compounded for use.
In examples 17 and 18, when the tea tree essential oil is replaced by the calendula essential oil and the chamomile essential oil respectively, the antibacterial rate of the calendula essential oil and the chamomile essential oil is slightly improved, which indicates that the antibacterial rate of the calendula essential oil and the chamomile essential oil is slightly higher than that of the tea tree essential oil, and in examples 12 and 13, when the calendula essential oil and the chamomile essential oil are compounded for use, the antibacterial rate is slightly higher than that in examples 17 and 18, which indicates that the antibacterial effect is better when the calendula essential oil and the chamomile essential oil are compounded for use; examples 14-16 further improved the bacteriostasis rate when calendula essential oil, chamomile essential oil were within the ratio of the present application, and were higher than single use of one essential oil, indicating that calendula essential oil, chamomile essential oil can synergistically increase under specific ratios, significantly improving bacteriostasis.
The degradation process of hydrogen peroxide is as follows: adding hydrogen peroxide into carboxymethyl chitosan with higher number average molecular weight under stirring to perform degradation reaction, adding alkali in the degradation reaction process to enable the pH of a reaction system to be 8.0-8.5, and adding sodium sulfite to stop the reaction until the viscosity of the reaction system is 80-120 cp.
The preparation method of the carboxymethyl chitosan comprises the step of carrying out acid precipitation washing on the carboxymethyl chitosan subjected to dioxygen water degradation.
The acid precipitation washing is to adjust the pH of the carboxymethyl chitosan material body degraded by hydrogen peroxide to 6-7 by acid, add ethanol until light yellow crystals are separated out, repeatedly wash by alcohol, dry by throwing, and dry to obtain carboxymethyl chitosan.
As can be seen from the combination of examples 1-11 and comparative examples 1-5 and the combination of Table 3, examples 1-3 are formulated with bioactive peptides, ocean glycoproteins and active polysaccharides, and the increase rate of skin elasticity is over 11% after continuous use for 15 days, which indicates that the antibacterial gel of the present application can enhance skin elasticity. When the bioactive peptide, the marine glycoprotein and the active polysaccharide are not added in the comparative example 1, the skin elasticity increasing rate is 0%, the skin elasticity increasing rate is increased to 3.5% after the bioactive peptide is added in the comparative example 2 on the basis of the comparative example 1, the skin elasticity increasing rate is increased to 2.2% after the marine glycoprotein is added in the comparative example 3 on the basis of the comparative example 1, the skin elasticity increasing rate is increased to 2.9% after the active polysaccharide is added in the comparative example 4 on the basis of the comparative example 1, which means that the bioactive peptide, the marine glycoprotein and the active polysaccharide can enhance the skin elasticity, and the skin elasticity increasing rate is increased to 10.6% after the bioactive peptide, the marine glycoprotein and the active polysaccharide are simultaneously added in the comparative example 5 on the basis of the comparative example 1, which is higher than that of the bioactive peptide, the marine glycoprotein and the active polysaccharide singly used, which means that the bioactive peptide, the marine glycoprotein and the active polysaccharide can synergistically increase the skin elasticity remarkably.
Examples 10 and 11 have slightly improved elasticity increasing rates when the Chinese angelica polysaccharide is replaced by the schizophyllan and the ginkgo polysaccharide respectively, which indicates that the schizophyllan and the ginkgo polysaccharide are slightly better than the Chinese angelica polysaccharide in the aspect of enhancing skin elasticity, and examples 5 and 6 have slightly higher elasticity increasing rates when the schizophyllan and the ginkgo polysaccharide are compounded and used, which indicates that the schizophyllan and the ginkgo polysaccharide are compounded and used, and further improved elasticity increasing rates; examples 7-9 further improved the rate of increase of elasticity when schizophyllan polysaccharide and ginkgo polysaccharide were in the ratio range of the present application, and were higher than the single use of one active polysaccharide, demonstrating that schizophyllan polysaccharide and ginkgo polysaccharide could synergistically increase the rate of increase of elasticity at the specific ratio.
The present embodiment is merely illustrative of the present application and is not intended to be limiting, and a person skilled in the art, after having read the present specification, may make modifications to the present embodiment without creative contribution as required, but is protected by patent laws within the scope of the claims of the present application.

Claims (8)

1. A bacteriostatic liquid is characterized by being prepared from the following raw materials in parts by weight
The method comprises the following steps:
0.1-0.3 part of bioactive peptide;
0.05-0.2 parts of marine glycoprotein;
2-4 parts of active polysaccharide;
15-20 parts of plant extract;
0.5-1.5 parts of sodium hyaluronate;
0.1-0.25 part of plant essential oil;
0.8-1.5 parts of lactic acid;
1-2 parts of carboxymethyl cellulose;
1-1.5 parts of glycerol;
1-2 parts of pH regulator;
0.5-1.5 parts of emulsifying agent;
purified water is added to 100 parts;
the plant extract is prepared from the following raw materials in parts by weight:
8-10 parts of kuh-seng;
6-8 parts of phellodendron;
2-4 parts of fructus cnidii;
4-6 parts of red sage root;
4-6 parts of safflower;
4-8 parts of motherwort;
1-2 parts of mugwort leaf;
the antibacterial liquid is an aqueous solution containing carboxymethyl chitosan, and the number average molecular weight of the carboxymethyl chitosan is 1000-20000.
2. A bacteriostatic liquid according to claim 1, characterized in that: the active polysaccharide consists of schizophyllan and ginkgo polysaccharide;
the concentration of the carboxymethyl chitosan aqueous solution is not less than 0.1wt%;
the carboxymethyl chitosan is O-carboxymethyl chitosan;
the preparation method of the carboxymethyl chitosan comprises the steps of degrading carboxymethyl chitosan raw materials with higher number average molecular weight through hydrogen peroxide, wherein the number average molecular weight of the carboxymethyl chitosan in the carboxymethyl chitosan raw materials with higher number average molecular weight is 30-70 ten thousand;
the carboxymethyl substitution degree in the carboxymethyl chitosan raw material with higher number average molecular weight is 0.8-1.3.
3. A bacteriostatic liquid according to claim 2, characterized in that: the schizophyllan and gingko polysaccharide are characterized by comprising the following components in parts by weight: (2-4).
4. A bacteriostatic liquid according to claim 1, characterized in that: the plant essential oil consists of calendula essential oil and chamomile essential oil;
the degradation process of hydrogen peroxide is as follows: adding hydrogen peroxide into carboxymethyl chitosan with higher number average molecular weight under stirring to perform degradation reaction, adding alkali in the degradation reaction process to enable the pH of a reaction system to be 8.0-8.5, and adding sodium sulfite to stop the reaction until the viscosity of the reaction system is 80-120 cp;
the preparation method of the carboxymethyl chitosan comprises the steps of carrying out acid precipitation washing on the carboxymethyl chitosan subjected to dioxygen water degradation;
the acid precipitation washing is to adjust the pH of the carboxymethyl chitosan material body degraded by hydrogen peroxide to 6-7 by acid, add ethanol until light yellow crystals are separated out, repeatedly wash by alcohol, dry by throwing, and dry to obtain carboxymethyl chitosan.
5. The bacteria inhibiting liquid of claim 4, wherein: the calendula essential oil and the chamomile essential oil are prepared from the following components in parts by weight: (1-3).
6. A bacteriostatic liquid according to claim 1, characterized in that: the antibacterial liquid is prepared from the following raw materials in parts by weight:
0.1 part of bioactive peptide;
0.2 parts of marine glycoprotein;
3 parts of active polysaccharide;
18 parts of a plant extract;
1 part of sodium hyaluronate;
0.2 parts of plant essential oil;
1 part of lactic acid;
1.5 parts of carboxymethyl cellulose;
glycerol 1.2 parts;
1.5 parts of pH regulator;
1 part of an emulsifying agent;
purified water is added to 100 parts;
the plant extract is prepared from the following raw materials in parts by weight:
9 parts of kuh-seng;
7 parts of phellodendron;
3 parts of fructus cnidii;
5 parts of red sage root;
4 parts of safflower;
6 parts of motherwort;
2 parts of mugwort leaf.
7. A method for preparing the antibacterial liquid according to any one of claims 1 to 6, characterized in that: the method comprises the following steps: step one, adding a proper amount of purified water into lactic acid, and dissolving to obtain a lactic acid solution;
pulverizing radix Sophorae Flavescentis, cortex Phellodendri, fructus Cnidii, saviae Miltiorrhizae radix, carthami flos, herba Leonuri and folium Artemisiae Argyi into coarse powder, and percolating to obtain plant extract;
dissolving carboxymethyl cellulose, bioactive peptide, active polysaccharide and sodium hyaluronate in a proper amount of purified water, standing for 10-12 hours to fully swell the carboxymethyl cellulose, and uniformly stirring to obtain a gel matrix;
step four, diluting the plant essential oil and the glycerol with a proper amount of ethanol, adding the emulsifier, and uniformly stirring to obtain essential oil mixture;
and fifthly, adding the mixture of the lactic acid solution, the plant extract and the essential oil into the gel matrix, uniformly stirring, adding purified water to a sufficient amount, slowly adding the pH regulator to the pH of 4-6 under the stirring state, and uniformly stirring to obtain the gel.
8. The method for preparing the antibacterial liquid according to claim 7, wherein: the second step specifically comprises the following steps: pulverizing radix Sophorae Flavescentis, cortex Phellodendri, fructus Cnidii, saviae Miltiorrhizae radix, carthami flos, herba Leonuri and folium Artemisiae Argyi into coarse powder, extracting by percolation method, using 55% ethanol as solvent, adding ethanol 2-3 times of the coarse powder, soaking for 5-7 days, slowly percolating, adding ethanol 3-4 times of the coarse powder, soaking for 3-5 days, slowly percolating, squeezing residues, mixing 2 percolates, mixing uniformly, decolorizing with active carbon, recovering ethanol, concentrating to relative density of 1.08-1.10 at 60deg.C, and obtaining plant extract.
CN202310278104.8A 2023-03-21 2023-03-21 Antibacterial liquid and preparation method thereof Withdrawn CN116270968A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310278104.8A CN116270968A (en) 2023-03-21 2023-03-21 Antibacterial liquid and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310278104.8A CN116270968A (en) 2023-03-21 2023-03-21 Antibacterial liquid and preparation method thereof

Publications (1)

Publication Number Publication Date
CN116270968A true CN116270968A (en) 2023-06-23

Family

ID=86779414

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310278104.8A Withdrawn CN116270968A (en) 2023-03-21 2023-03-21 Antibacterial liquid and preparation method thereof

Country Status (1)

Country Link
CN (1) CN116270968A (en)

Similar Documents

Publication Publication Date Title
Morton Folk uses and commercial exploitation of Aloe leaf pulp
CN107616956A (en) A kind of aloe skin care compositions and preparation method thereof
KR101878450B1 (en) Tamarind seed polysaccharide for use in the treatment of microbial infections
CN112206308A (en) Nourishing and bacteriostatic gel and preparation method thereof
CN109350579B (en) Whitening cosmetic additive, whitening skin-refreshing lotion and preparation method thereof
CN113520956B (en) Acne removing mask with common bombax flower extract and radix sophorae flavescentis extract as active ingredients and preparation method thereof
CN108836880A (en) A kind of Baby Care cream and preparation method thereof containing nano-silver ionic
CN115317417A (en) Composition containing guaiacum extract for repairing acne sensitive muscle and skin care product thereof
CN112656709A (en) Skin care compositions and uses thereof
CN109864943B (en) Preparation method and application of bletilla striata polyphenol
CN110090185A (en) A kind of method that Herba Saussureae Involueratae extract prepares facial mask
CN106562921A (en) Liquid oral composition containing TCM active substances and tea tree essential oil
CN111437225A (en) Hemostatic, granulation-promoting and antibacterial composition and application thereof
CN111973515A (en) Bacteriostatic restoration plant extraction composition, preparation method and application thereof
CN114432225A (en) Sunscreen skin care product and preparation method thereof
CN108186426B (en) Antibacterial mask liquid, mask and preparation method of mask liquid
KR20220111000A (en) Cosmetic composition for regenerating skin and improving skin wrinkle
KR101420211B1 (en) Skin Composition for External Application Using Bud and Sprout Extract
CN116270968A (en) Antibacterial liquid and preparation method thereof
KR101427462B1 (en) Composite Natural Antiseptics From Forsythia suspensa Vahl, Leonurus sibiricus L., Crataegi fructus and Akebia quinata Decaisne, Cosmetic Compositions Containing Them
CN105193686A (en) Female care solution and preparation method thereof
CN112274462B (en) Composite bacteriostatic agent with bacteriostatic and epidermal moisturizing effects and preparation and application thereof
CN112870140A (en) Anti-allergy itching-relieving composition, skin-care gel, and preparation method and application thereof
CN112353867A (en) Natural plant bacteriostatic spray and preparation method thereof
CN111905030A (en) Vagina health care gel and preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20230623

WW01 Invention patent application withdrawn after publication