CN116210533A

CN116210533A - Seedling raising method for wild Limonium bicolor

Info

Publication number: CN116210533A
Application number: CN202211736875.9A
Authority: CN
Inventors: 刘桂霞; 王星然; 庄浩梁; 刘振; 魏少翀
Original assignee: Hebei University
Current assignee: Hebei University
Priority date: 2022-12-31
Filing date: 2022-12-31
Publication date: 2023-06-06
Anticipated expiration: 2042-12-31
Also published as: CN116210533B

Abstract

The invention discloses a seedling raising method of wild Limonium bicolor. The seedling raising method combines novel biological fertilizer seed soaking and spraying under the sowing depth of 0.5-1cm, namely seed soaking and leaf spraying are carried out by adopting amino acid fertilizer diluent or algae extract diluent with specific concentration, so that the planting cost is reduced, the seedling emergence rate is increased, and the seedling resistance is improved. The seed soaking increases the absorption of the seeds to nutrient substances, thereby promoting the germination of the seeds and increasing the emergence rate and the survival rate; the spraying treatment can promote the growth of the wild bicolor limonium, improve the growth speed of the wild bicolor limonium, enable the plant leaves to be long, wide and thick, be bright in color, increase the crown width and biomass, and provide technical support for the development and utilization of the wild bicolor limonium.

Description

Seedling raising method for wild Limonium bicolor

Technical Field

The invention relates to a seedling raising method, in particular to a seedling raising method of wild Limonium bicolor.

Background

The wild Limonium bicolor (bag.) Kuntze is Lan Xueke, which is a perennial herb of Limonium genus, and is mainly distributed in northern and eastern coastal areas of China. The wild Limonium bicolor is an excellent ornamental flower, has higher medicinal value, can be used as high-quality pasture when not flowering, and is an excellent sand-fixing plant.

Currently, the limonium bicolor is mostly a wild variety, and is collected manually for reprocessing and selling or used in other aspects. However, as the artificial damage and the natural environment are degraded, the resource amount of the wild Limonium bicolor is continuously reduced, so that supply and demand are not required, and the social requirement cannot be met. Although the artificial cultivation and domestication work of the wild limonium bicolor has been tried, the artificial breeding often has the problems of low emergence rate, slow growth in seedling stage and weak growth due to the too small seeds of the wild limonium bicolor, so that the wild limonium bicolor rarely enters the flower market, and large-area self-propagation is difficult to carry out in the wild.

In plant growth promotion, seeds are generally soaked by seed soaking liquid before the seeds are grown, or various bactericides, pesticides, herbicides, plant hormones and the like are used for protection in the growing process. However, the seed soaking solutions and various growth-promoting regulators used in the prior art are too complex in composition, various in variety and different in concentration, and particularly, the application of the binary-color limonium which is too small in seed is not described in detail. Even if the conventional seed soaking liquid is used, the defect of slow seed germination and poor seed germination effect still exists. Moreover, the improper use of various reagents in the seedling stage can also lead to low emergence rate, slow growth of seedlings, high death rate and the like, and cause damage to the seeds and seedlings of the wild Limonium bicolor, thereby generating unexpected side effects.

Disclosure of Invention

The invention aims to provide a seedling raising method of wild limonium bicolor, which aims to solve the problems of low emergence rate, slow seedling growth and weak resistance of the wild limonium bicolor artificial breeding method.

The purpose of the invention is realized in the following way:

a seedling raising method of wild limonium bicolor, comprising the following steps:

s1, seed soaking: mixing the seeds of the wild Limonium bicolor with 0.1-0.2% of algae extract diluent or 0.1-0.3% of amino acid fertilizer diluent, soaking the seeds for 12-16 hours;

wherein the mass ratio of the seeds to the algae extract diluent or amino acid fertilizer diluent is 1:10-15.

S2, sowing: after seed soaking, draining the seeds, drying in the shade, and then dibbling the seeds into seedling raising soil in a nursery, wherein the sowing depth is 0.5-1cm.

S3, spraying the back surfaces of the leaves: when the seeds grow to 4-5 leaves, the algae extract diluent or the amino acid fertilizer diluent is sprayed, wherein the spraying time is before 10 am or after 5 pm, the spraying position is the back of the leaves, the spraying is performed for 3 times, 10 days are spaced between every two spraying, and the spraying is performed until the back of the leaves is completely wetted.

The seedling raising method of the wild Limonium bicolor of the invention also comprises the following steps:

s4, maintaining the temperature of the seedling raising environment at 28-32 ℃, and keeping the seedling raising soil moist to enable the seedling raising soil to be held by hands to form clusters, and then dispersing the clusters in one touch; timely weed removal, and window ventilation when the temperature reaches above 30 ℃ and the humidity is 60% -70%. And transplanting the seedling to a field when the leaf length of the seedling is greater than 10 cm and the leaf width is greater than 3 cm.

Preferably, in step S1, seeds of the wild Limonium bicolor are mixed with an amino acid fertilizer diluent with a concentration of 0.2% and soaked for 16 hours.

Preferably, in step S3, leaf back spraying is performed using an algae extract at a concentration of 0.1%.

Preferably, the algae extract comprises alginic acid, mannitol, biological enzyme, and plant hormone.

Preferably, the effective components of the amino acid fertilizer comprise 13.9% of organic matters, 2.3% of total nitrogen and 18 amino acids;

wherein, the active ingredients of the amino acid fertilizer comprise 18 amino acid components and contents of the amino acid components are respectively as follows: 530.8mg/100g aspartic acid, 1615.6mg/100g glutamic acid, 29.9mg/100g cystine, 307.8mg/100g serine, 157.3mg/100g histidine, 768.9mg/100g glycine, 180.8mg/100g threonine, 634.1mg/100g arginine, 804.9mg/100g alanine, 242.7mg/100g tyrosine, 557.6mg/100g valine, 102.3mg/100g methionine, 1446.1mg/100g tryptophan, 343.1mg/100g phenylalanine, 357.3mg/100g isoleucine, 804.5mg/100g leucine, 500.2mg/100g lysine, 388.4mg/100g proline.

Preferably, the amino acid fertilizer is purchased from taiwan femto fertilizer company, product accession number: fertilizer (quality) character 0676014.

The seedling raising method of the invention determines proper dibbling depth according to the characteristics of the wild Limonium bicolor seeds, and combines novel biological fertilizer seed soaking with leaf surface spraying. The amino acid fertilizer diluent or the algae extract diluent with specific concentration is selected for seed soaking or leaf surface spraying, so that on one hand, the seed vigor can be excited in the early stage of seedling raising, the content of nutrient substances in the seed is increased, and the soil structure is improved, thereby rapidly breaking dormancy of the seed and improving the germination rate and germination speed; on the other hand, the amino acid fertilizer and the algae extract are used as growth regulators, and are sprayed on leaf surfaces in the later period of seedling cultivation, so that a series of problems caused by insufficient nutrients in early growth of seedling plants can be further overcome, the chlorophyll content is improved, the crown size, biomass and the like are increased, and the plant resistance is improved.

The amino acid fertilizer diluent or algae extract diluent adopted by the invention is mainly prepared from organic matters, so that the use method is simple, the price is relatively low, and the planting cost is reduced.

The amino acid fertilizer and the algae extract adopted by the invention are biological fertilizers, so that the chemical fertilizers are replaced, the pollution of the atmosphere and the soil is avoided, and the health and the ecological environmental safety of human beings are ensured.

Drawings

FIG. 1 is a graph showing the effect of seed soaking treatment on the emergence rate of wild Limonium bicolor; wherein a: soaking seeds of the algae extract; b: seed soaking treatment of amino acid fertilizer; c: seed soaking treatment of brassinolide. The different capital letters in the figure represent the obvious difference between the emergence rates of the wild Limonium bicolor with different seed soaking concentrationsp< 0.05); in the figure, different lowercase letters represent obvious difference between the emergence rates of the wild Limonium bicolor with different seed soaking timep＜0.05）。

FIG. 2 is a graph showing the effect of different seed soaking treatments on the crown amplitude of wild Limonium bicolor; wherein a: soaking seeds of the algae extract; b: seed soaking treatment of amino acid fertilizer; c: seed soaking treatment of brassinolide. The different capital letters in the figure represent the obvious difference between the emergence rates of the wild Limonium bicolor with different seed soaking concentrationsp< 0.05); in the figure, different lowercase letters represent obvious difference between the emergence rates of the wild Limonium bicolor with different seed soaking timep＜0.05）。

FIG. 3 is a graph showing the effect of different seed soaking treatments on wild Limonium bicolor aboveground biomass; wherein a: soaking seeds of the algae extract; b: seed soaking treatment of amino acid fertilizer; c: seed soaking treatment of brassinolide. The different capital letters in the figure represent the obvious difference between the emergence rates of the wild Limonium bicolor with different seed soaking concentrationsp< 0.05); in the figure, different lowercase letters represent obvious difference between the emergence rates of the wild Limonium bicolor with different seed soaking timep＜0.05）。

FIG. 4 is a graph of the effect of 0.1% algae extract dilution spray on the growth of Limonium bicolor seedlings, wherein A: a control group; b:0.1% algae extract spray treatment group.

Detailed Description

The invention will be further illustrated with reference to specific examples.

Test site: in the greenhouse of the Shangde Fengning county fish, shangzhen national fish, shangshan pasture

The amino acid fertilizer is tan and is purchased from Feihong fertilizer Co., ltd; algae extract was dark gray, purchased from Hangzhou ocean technologies, inc.; brassinolide was purchased from Shanghai Annotation laboratory technology Co., ltd.

Example 1

A method for raising seedlings of wild Limonium bicolor, comprising the following steps:

s1, seed soaking: and (3) selecting an amino acid fertilizer diluent to carry out seed soaking treatment on the seeds of the wild Limonium bicolor. The algae extract diluent is used for soaking seeds of the wild Limonium bicolor. The algae extract was set at 4 dilution concentrations (0.10%, 0.20%, 0.30%, 0.40%), each concentration was set at 3 seed soaking times (8 hours, 16 hours, 24 hours); each treatment was repeated 4 times.

S2, sowing: and (5) draining the seeds in time after the seeds are soaked for a specified time, and sowing the seeds in a seedling pot after the seeds are dried in the shade, wherein the sowing depth is 0.8cm.

S3, leaf surface spraying: when the seeds grow to 4-5 leaves, 0.10% of algae extract diluent is sprayed, the spraying time is before 10 am or after 5 pm, the spraying position is the leaf surface, 3 times of spraying are continuously performed every 10 days/time, and each time of spraying is performed until the leaves are wet.

S4, daily management: in the seedling raising process of the wild Limonium bicolor, the temperature of the seedling raising environment is kept at 28-32 ℃, and seedling raising soil is kept moist, so that the seedlings can be held by hands to form clusters, and can be dispersed in one touch; timely weed removal, and window ventilation when the temperature reaches above 30 ℃ and the humidity is 60% -70%. Transplanting the seedling to a field when the leaf length of the seedling is more than 10 cm and the leaf width is more than 3 cm.

Example 2

A method of raising seedlings of wild Limonium bicolor, said method being substantially the same as in example 1, except that: 4 dilution concentrations (0.10%, 0.20%, 0.30%, 0.40%) were set using amino acid fertilizer dilutions, and 3 seed soaking times (8 hours, 16 hours, 24 hours) were set for each concentration, with 4 replicates for each treatment.

Example 3

A method of raising seedlings of wild Limonium bicolor, said method being substantially the same as in example 1, except that: the seed soaking treatment is carried out on the seeds of the wild limonium bicolor by using brassinolide. The concentrations of the brassinolide set dilutions are 0.02mg/L, 0.04mg/L, 0.06mg/L and 0.08mg/L respectively; 3 seed soaking times (8 hours, 16 hours, 24 hours) are set for each concentration; each treatment was repeated 4 times.

Example 4

A method of raising seedlings of wild Limonium bicolor, said method being substantially the same as in example 1, except that: soaking seeds of the wild Limonium bicolor with warm water, and setting 3 soaking times (8 hours, 16 hours, 24 hours); each treatment was repeated 4 times.

In the research process, example 4 is compared with example 1, example 2 and example 3 respectively, and the comparison results are shown in fig. 1, fig. 2 and fig. 3.

As can be seen from FIG. 1, the effects of the algae extract dilution and the amino acid fertilizer dilution on the emergence rate of the wild Limonium bicolor after seed soaking treatment are different, but both have promoting effects. The brassinolide treatment and the control are obviously reduced or have no obvious difference, and the brassinolide treatment and the control have no promotion effect on the emergence rate of the wild limonium bicolor.

In the seed soaking treatment for 8 hours, after seed soaking is carried out by 0.2 percent of algae extract diluent, the emergence rate is superior to that of the control and other treatments, and the emergence rate of the 0.2 percent of algae extract diluent seed soaking is improved by 76.1 percent compared with that of the control. In the seed soaking 16h treatment, the seed soaking emergence rate of the 0.2% amino acid fertilizer diluent is obviously improved compared with the control and other treatments, and is improved by 216.5% compared with the control emergence rate. In the seed soaking treatment for 24 hours, after seed soaking is carried out by 0.2% of amino acid fertilizer diluent and 0.3% of algae extract diluent, the emergence rate is obviously superior to that of a control and other treatments, but no obvious difference exists between the two treatments, the 0.2% of amino acid fertilizer treatment is higher than that of the 0.3% of algae extract treatment, and the emergence rate of the 0.2% of amino acid fertilizer seed soaking is improved by 159.3% compared with that of the control.

As can be seen from fig. 2, the algae extract dilution seed soaking generally has no significant effect on the crown amplitude of the wild limonium bicolor and the control. The crown width shows a trend of ascending and descending along with the increase of seed soaking time of 0.1 percent and 0.2 percent of amino acid fertilizer diluent seed soaking, and the crown width treated by 16h of amino acid fertilizer diluent seed soaking is obviously superior to the crown width treated by 8h and 24h of seed soaking. In the 16h treatment of amino acid fertilizer seed soaking, the 0.2% seed soaking treatment crown width is obviously superior to that of the control and other treatments. Except that the difference between 0.1% brassinolide seed soaking for 24 hours and the control is not obvious and is slightly lower than the control, the rest treatments are obviously reduced compared with the control, and the treatment has no promotion effect on the crown amplitude of the wild limonium bicolor.

As can be seen from FIG. 3, the algae extract dilution and the amino acid fertilizer dilution seed soaking have different promotion effects on the above-ground biomass of the wild Limonium bicolor. Exogenous application of brassinolide each treatment had no promoting effect on the aboveground biomass of wild limonium bicolor. In the seed soaking 16h treatment, the 0.2% amino acid fertilizer soaked overground biomass is obviously superior to other treatments and controls, and is improved by 157.9% compared with the control overground biomass. In the seed soaking 8h treatment, the above ground biomass of 0.2% of the algae extract is increased by 78.1% compared with the control above ground biomass.

From the results, the 0.2% amino acid fertilizer diluent can effectively improve the emergence rate of wild bicolor Chinese yam seeds after soaking the seeds for 16 hours, and the effect is optimal.

Example 5

s1, seed soaking: soaking seeds of the wild Limonium bicolor with 0.20% of amino acid fertilizer diluent for 16 hours.

S3, leaf surface spraying: when the seeds grow to 4-5 leaves, the diluted solution of the algae extract is selected for foliage spraying. The algae extract dilutions were set to 4 dilution concentrations (0.10%, 0.20%, 0.30%, 0.40%), each treatment was repeated 4 times, the spraying time was before 10 am or after 5 pm, the spraying position was the leaf surface, 3 times per 10 days/time, each pot was sprayed to the back of the leaf for wetting.

S4, daily management: in the seedling raising process of the wild Limonium bicolor, the temperature of the seedling raising environment is kept at 28-32 ℃, and seedling raising soil is kept moist, so that the seedlings can be held by hands to form clusters, and can be dispersed in one touch; timely weed removal, and window ventilation when the temperature reaches above 30 ℃ and the humidity is 60% -70%. And transplanting the seedling to a field when the leaf length of the seedling is greater than 10 cm and the leaf width is greater than 3 cm.

Example 6

A method of raising seedlings of wild limonium bicolor, the method being substantially the same as in example 5, except that: and (5) selecting amino acid fertilizer diluent for foliage spraying. The amino acid fertilizer was set at 4 dilution concentrations (0.10%, 0.20%, 0.30%, 0.40%), 4 replicates per treatment.

Example 7

A method of raising seedlings of wild limonium bicolor, the method being substantially the same as in example 5, except that: and (3) selecting brassinolide for foliage spraying. The Brassinolide (BR) dilutions were set to 0.02mg/L, 0.04mg/L, 0.06mg/L and 0.08mg/L, respectively, with 4 replicates per treatment.

Example 8

A method of raising seedlings of wild limonium bicolor, the method being substantially the same as in example 5, except that: spraying is carried out without using any reagent.

The present invention was compared with examples 8, 5, 6 and 7 during the course of the study, and the comparison results are shown in table 1.

As can be seen from table 1, the effect of the algae extract dilution and the amino acid fertilizer dilution on promoting the growth shape of the wild limonium bicolor by spraying the leaf surface is significantly higher than that of the control and other treatments. The algae extract diluent has the most obvious promotion effect on the growth shape of seedlings, including plant height, growth speed, leaf length, crown breadth, root base diameter, biomass, leaf width, chlorophyll a content, chlorophyll b content, total chlorophyll content and the like, which are all obviously higher than those of other treatments. The promotion effect of the three different concentrations of plant growth promoting substances was shown by 0.1% algae extract treatment >0.4% and 0.1% amino acid fertilizer treatment >0.06mg/L and 0.08mg/L brassinolide treatment. The algae extract with the concentration of 0.1 percent is sprayed to obviously improve the plant height, the growth speed and the crown width of the wild limonium bicolor seedlings by 167.9 percent, 70.0 percent and 106.9 percent respectively compared with the control. And as can be seen from fig. 4, the seedlings grown after spraying with 0.1% algae extract are far better than the control.

Therefore, the 0.10% algae extract diluent can effectively improve the early growth condition of the wild Limonium bicolor seedlings by spraying, and the effect is optimal.

Table 1.1 comparison of optimal spray treatment groups for three plant growth promoting substances

Table 1.2 comparison of optimal spray treatment groups for three plant growth promoting substances

Table 1.3 comparison of optimal spray treatment groups for three plant growth promoting substances

Claims

1. The seedling raising method of the wild Limonium bicolor is characterized by comprising the following steps of:

s2, sowing: after seed soaking is completed, draining seeds and drying in the shade, and dibbling the dried seeds into seedling raising soil in a nursery, wherein the sowing depth is 0.5-1cm;

s3, spraying the back surfaces of the leaves: when the seeds grow to 4-5 leaves, algae extract diluent with the concentration of 0.1-0.2% or amino acid fertilizer diluent with the concentration of 0.1-0.3% is sprayed, wherein the spraying time is before 10 am or after 5 pm, the spraying position is the back of the leaves, the front and back spraying is performed for 3 times, the interval between every two spraying is 10 days, and each spraying is performed until the back of the leaves is completely wetted.

2. The method for raising seedlings of wild Limonium bicolor according to claim 1, further comprising:

s4, transplanting the seedling to a field when the leaf length of the seedling is greater than 10 cm and the leaf width is greater than 3 cm.

3. The method for raising seedlings of wild type Limonium bicolor according to claim 1 or 2, wherein in step S1, seeds of wild type Limonium bicolor are mixed with an amino acid fertilizer diluent having a concentration of 0.2% and soaked for 16 hours.

4. The method for raising seedlings of wild type limonium bicolor according to claim 1 or 2, wherein in step S3, leaf back spraying is performed using an algae extract at a concentration of 0.1%.

5. The method for raising seedlings of wild limonium bicolor according to claim 1 or 2, wherein in step S1, the mass ratio of the seeds to the algae extract dilution or the amino acid fertilizer dilution is 1:10-15.

6. The method for raising seedlings of wild type limonium bicolor according to claim 1 or 2, wherein the effective components of the algae extract include alginic acid, mannitol, biological enzymes and plant hormones.

7. The method for raising seedlings of wild Limonium bicolor according to claim 1 or 2, wherein the effective components of the amino acid fertilizer comprise 13.9% of organic matter, 2.3% of total nitrogen and 18 amino acids.

8. The method for raising seedlings of wild type limonium bichromaticum according to claim 7, wherein the specific components and contents of 18 amino acids in the active ingredients of the amino acid fertilizer are 530.8mg/100g aspartic acid, 1615.6mg/100g glutamic acid, 29.9mg/100g cystine, 307.8mg/100g serine, 157.3mg/100g histidine, 768.9mg/100g glycine, 180.8mg/100g threonine, 634.1mg/100g arginine, 804.9mg/100g alanine, 242.7mg/100g tyrosine, 557.6mg/100g valine, 102.3mg/100g methionine, 1446.1mg/100g tryptophan, 343.1mg/100g phenylalanine, 357.3mg/100g isoleucine, 804.5mg/100g leucine, 500.2mg/100g lysine and 388.4mg/100g proline, respectively.

9. The method for raising seedlings of wild limonium bicolor according to claim 1 or 2, wherein in the process of raising seedlings of wild limonium bicolor, the temperature of the seedling raising environment is kept at 28-32 ℃, and seedling raising soil is kept moist; when the temperature reaches above 30 ℃ and/or the humidity reaches 60% -70%, the nursery is windowed and ventilated.