CN116196281A - Preparation method of water-soluble powder of cordyceps militaris extract - Google Patents
Preparation method of water-soluble powder of cordyceps militaris extract Download PDFInfo
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- CN116196281A CN116196281A CN202310157755.1A CN202310157755A CN116196281A CN 116196281 A CN116196281 A CN 116196281A CN 202310157755 A CN202310157755 A CN 202310157755A CN 116196281 A CN116196281 A CN 116196281A
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- cordyceps militaris
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- militaris extract
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 70
- 239000000284 extract Substances 0.000 title claims abstract description 33
- 239000004552 water soluble powder Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 238000000605 extraction Methods 0.000 claims abstract description 20
- 238000001694 spray drying Methods 0.000 claims abstract description 14
- 238000007670 refining Methods 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 239000000706 filtrate Substances 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 14
- 239000011362 coarse particle Substances 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 10
- 239000000919 ceramic Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 239000008213 purified water Substances 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 claims description 6
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 6
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 6
- 229940069328 povidone Drugs 0.000 claims description 6
- 229920000858 Cyclodextrin Polymers 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 2
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- 239000003814 drug Substances 0.000 abstract description 6
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- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
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- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 1
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
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- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
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Abstract
The invention belongs to the technical field of medicines, and particularly relates to a preparation method of water-soluble powder of cordyceps militaris extract. The preparation method comprises the steps of raw material selection, coarse crushing, hot extraction, refining, concentration and spray drying, wherein the effective components of the cordyceps militaris are greatly reserved by precisely controlling the processing temperature and time, the content of the active components is increased, the water-soluble powder with high component content and small administration dosage is obtained by spray drying, and the dissolution rate of powder substances is improved. The water-soluble powder of the Cordyceps militaris extract obtained by the invention can be prepared into medicines, health products, compound preparations or used as food additives.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a preparation method of water-soluble powder of cordyceps militaris extract.
Background
Cordyceps militaris, also known as Cordyceps militaris (Cordyceps militaris (L.) Link), is a large-scale edible and pharmaceutical fungus belonging to the phylum Eumycota (Eumycota), ascomycota (Ascomycota), ascomycotina (Ascomycotina), hypsizyga (Sordariomycetes), hypocrea (Hypocrea), hypocrea (Hypoxeromycetaceae), cordycepa (Cordyceps). Cordyceps militaris and Cordyceps sinensis have similar active ingredients, and contain cordycepin, cordycepic acid, cordyceps polysaccharide, ergosterol, amino acid, nucleoside substances, etc., and have similar effects to Cordyceps sinensis. The Chinese medicine considers that the cordyceps sinensis enters the lung and kidney channels, can tonify lung yin and kidney yang, and can balance and regulate yin and yang. Recent researches show that the cordyceps militaris has rich protein content, contains complete amino acids necessary for human body, has sufficient quantity and proper proportion, has good edible value, and the main components of the cordyceps militaris have the effects of expanding trachea, calming, resisting bacteria, reducing blood pressure, reducing blood fat, improving blood circulation, enhancing human body immunity and the like. In view of the high activity of Cordyceps militaris, the national health commission release bulletin approves Cordyceps militaris as a new food material.
At present, most of literature patents related to cordyceps militaris extracts are laboratory or small-scale achievements or related technical methods for simply applying other extracts, and the problems of complex raw material treatment, intense process, complicated working procedures, poor continuity, high production cost and the like exist. Through patent document search, patent number cn201310207185.9, a preparation method of Cordyceps militaris instant powder, the main process of the Cordyceps militaris instant powder is prepared through hot water extraction, homogenization, enzymolysis, wet ultrafine grinding, homogenization and spray drying, the production process has higher energy consumption, more complicated working procedures, difficult realization of continuity, poor rehydration clarity of the product and limited application range.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a preparation method of water-soluble powder of cordyceps militaris extract, which has the advantages of simple process, low energy consumption, high active ingredient content and easy dissolution of active ingredients.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
a preparation method of water-soluble powder of Cordyceps militaris extract comprises the following steps:
s1: raw material selection: selecting full and dry cordyceps militaris fruiting bodies;
s2: coarse crushing: crushing the dried cordyceps militaris fruiting bodies in crushing equipment to obtain coarse cordyceps militaris particles with the length of 2-6 mm;
s3: and (3) heat extraction: adding purified water with the weight of 10-20 times of that of the cordyceps militaris coarse particles, soaking for 60-90 min, heating to 80-100 ℃ and carrying out reflux heat extraction to obtain an extracting solution;
s4: refining: filtering and refining the obtained extracting solution by using ceramic membrane equipment with the aperture of 100-1000 nm to obtain refined filtrate;
s5: concentrating: concentrating the obtained fine filtration filtrate at the temperature of 50-60 ℃ and the vacuum degree of less than or equal to minus 0.1MPa to obtain a concentrated solution, wherein the concentration of the solid matters of the concentrated solution is 10-20%;
s6: spray drying: and adding the dissolution-assisting food auxiliary materials with the weight 1-3 times of the solid matters into the concentrated solution, uniformly stirring, and performing spray drying to obtain the Cordyceps militaris extract water-soluble powder.
In the preparation method of the water-soluble powder of the cordyceps militaris extract, in the step S3, the heat extraction time is 1-3 times, and the extraction time is 100-150 minutes each time.
The preparation method of the water-soluble powder of the cordyceps militaris extract comprises the following step S4: centrifuging the obtained extract for 10-20 min at 2000-3000 r/min, taking out supernatant, and selecting supernatant for refining.
The preparation method of the water-soluble powder of the cordyceps militaris extract comprises the step of taking out the supernatant, and inputting the extract into a heat extraction device in the step S3 again for circulating reflux heat extraction operation.
In the preparation method of the water-soluble powder of the cordyceps militaris extract, the refined filtrate obtained in the step S4 is concentrated in vacuum concentration equipment.
In the preparation method of the water-soluble powder of the cordyceps militaris extract, the auxiliary materials of the dissolution-assisting food in the step S6 comprise cyclodextrin and povidone.
Advantageous effects
The invention has the advantages that the processing temperature and time are accurately controlled through the process steps of heat extraction, fine filtration, concentration and the like, the active ingredients of the cordyceps militaris are greatly reserved, the content of the active ingredients is increased, the water-soluble powder with high ingredient content and small administration dosage is obtained through spray drying, the dissolution rate of powder substances is improved, and the dried raw materials can be prepared into medicines, health-care products, compound preparations or food additives.
Drawings
FIG. 1 is a process flow diagram of the present invention.
Detailed Description
In order to explain the technical scheme of the invention more precisely and accurately, the following description is given by way of example so that the technical principle, the process flow and the process parameter control of the invention can be accurately understood by those skilled in the art.
Example 1
Referring to fig. 1, this embodiment discloses a preparation method of water-soluble powder of Cordyceps militaris extract, comprising the following steps:
firstly, selecting raw materials, selecting full and dry cordyceps militaris fruiting bodies as main raw materials, and weighing 100g of cordyceps militaris fruiting bodies; crushing the dried cordyceps militaris fruiting bodies in crushing equipment to obtain cordyceps militaris coarse particles with the length of 2 mm; adding purified water 10 times of the weight of the Cordyceps militaris coarse particles, soaking for 60min, heating to 80deg.C, and reflux-extracting for 100min to obtain extractive solution with extraction times of 1 time; centrifuging the obtained extractive solution at 2000r/min for 10min, taking out supernatant, and filtering and refining the obtained supernatant with ceramic membrane equipment with aperture of 1000nm to obtain fine filtrate; concentrating the obtained fine filtration filtrate in vacuum concentration equipment at the temperature of 50 ℃ and the vacuum degree of-0.1 MPa to obtain concentrated solution, wherein the solid concentration of the concentrated solution is 10%; adding cyclodextrin which is an auxiliary material of dissolution-assisting food and has the weight of 1 time of the solid into the concentrated solution, uniformly stirring, and performing spray drying to obtain the water-soluble powder of the Cordyceps militaris extract.
Example 2
The difference between this example and example 1 is that 100g of full and dry cordyceps militaris fruiting body is weighed; crushing the dried cordyceps militaris fruiting bodies in crushing equipment to obtain cordyceps militaris coarse particles with the length of 5 mm; adding purified water 15 times of the weight of the Cordyceps militaris coarse particles, soaking for 80min, heating to 85deg.C, and reflux-extracting for 120min for 2 times; centrifuging the obtained extractive solution at 2500r/min for 12min, taking out supernatant, and filtering and refining the obtained supernatant with ceramic membrane equipment with aperture of 500nm to obtain fine filtrate; concentrating the obtained fine filtration filtrate in vacuum concentration equipment at the temperature of 60 ℃ and the vacuum degree of-0.15 MPa to obtain a concentrated solution, wherein the solid concentration of the concentrated solution is 16%; and adding povidone serving as a dissolution-assisting food auxiliary material, wherein the weight of povidone is 2 times of that of the solid matters, uniformly stirring, and performing spray drying to obtain the cordyceps militaris extract water-soluble powder.
Example 3
The difference between this example and example 1 is that 100g of full and dry cordyceps militaris fruiting body is weighed; crushing the dried cordyceps militaris fruiting bodies in crushing equipment to obtain cordyceps militaris coarse particles with the length of 6 mm; adding purified water with the weight 20 times of that of the Cordyceps militaris coarse particles, soaking for 90min, heating to 100deg.C, and reflux-extracting to obtain extractive solution with the extraction time of 3 times and 150min each time; centrifuging the obtained extractive solution at 3000r/min for 20min, taking out supernatant, and filtering and refining the obtained supernatant with ceramic membrane equipment with aperture of 1000nm to obtain fine filtrate; concentrating the obtained fine filtration filtrate at 55 ℃ and vacuum degree of-0.12 MPa to obtain concentrated solution, wherein the concentration of the solid matters of the concentrated solution is 20%; adding cyclodextrin which is an auxiliary material of dissolution-assisting food and is 3 times of the solid weight into the concentrated solution, uniformly stirring, and performing spray drying to obtain the water-soluble powder of the Cordyceps militaris extract.
Example 4
The difference between this example and example 2 is that 100g of full and dry cordyceps militaris fruiting body is weighed; crushing the dried cordyceps militaris fruiting bodies in crushing equipment to obtain cordyceps militaris coarse particles with the length of 5 mm; adding purified water 15 times of the weight of the Cordyceps militaris coarse particles, soaking for 80min, heating to 85deg.C, and reflux-extracting for 120min for 2 times; centrifuging the obtained extractive solution at 3000r/min for 20min, taking out supernatant, filtering and refining the obtained extractive solution supernatant with ceramic membrane equipment with aperture of 500nm to obtain fine filtrate, taking out supernatant, and re-inputting the extractive solution into a heat extraction device for circulation reflux heat extraction operation to fully extract effective components of Cordyceps militaris. Concentrating the obtained fine filtration filtrate in vacuum concentration equipment at the temperature of 60 ℃ and the vacuum degree of-0.15 MPa to obtain a concentrated solution, wherein the solid concentration of the concentrated solution is 16%; and adding povidone serving as a dissolution-assisting food auxiliary material, wherein the weight of povidone is 2 times of that of the solid matters, uniformly stirring, and performing spray drying to obtain the cordyceps militaris extract water-soluble powder.
Example 5
The difference between this example and example 3 is that 100g of full and dry cordyceps militaris fruiting body is weighed; crushing the dried cordyceps militaris fruiting bodies in crushing equipment to obtain cordyceps militaris coarse particles with the length of 6 mm; adding purified water with the weight 20 times of that of the Cordyceps militaris coarse particles, soaking for 90min, heating to 100deg.C, and reflux-extracting to obtain extractive solution with the extraction time of 3 times and 150min each time; filtering and refining the obtained extracting solution by using ceramic membrane equipment with the aperture of 1000nm to obtain refined filtrate; concentrating the obtained fine filtration filtrate at 55 ℃ and vacuum degree of-0.12 MPa to obtain concentrated solution, wherein the concentration of the solid matters of the concentrated solution is 20%; adding cyclodextrin which is an auxiliary material of dissolution-assisting food and is 3 times of the solid weight into the concentrated solution, uniformly stirring, and performing spray drying to obtain the water-soluble powder of the Cordyceps militaris extract.
The invention has the advantages that the processing temperature and time are accurately controlled through the process steps of hot extraction, fine filtration, concentration and the like, the active ingredients of the cordyceps militaris are greatly reserved, the content of the active ingredients is increased, the water-soluble powder with high ingredient content and small administration dosage is obtained through spray drying, the dissolution rate of powder substances is improved, and the dried raw materials can be prepared into medicines, health-care products, compound preparations or used as food additives, and can be conventionally prepared into tablets, capsules, granules, chewing agents and the like.
The foregoing description is only of the preferred embodiments of the present invention and is not intended to limit the scope of the invention, and all equivalent structural changes made by the description of the present invention and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the invention.
Claims (6)
1. A preparation method of water-soluble powder of cordyceps militaris extract is characterized by comprising the following steps:
s1: raw material selection: selecting full and dry cordyceps militaris fruiting bodies;
s2: coarse crushing: crushing the dried cordyceps militaris fruiting bodies in crushing equipment to obtain coarse cordyceps militaris particles with the length of 2-6 mm;
s3: and (3) heat extraction: adding purified water with the weight of 10-20 times of that of the cordyceps militaris coarse particles, soaking for 60-90 min, heating to 80-100 ℃ and carrying out reflux heat extraction to obtain an extracting solution;
s4: refining: filtering and refining the obtained extracting solution by using ceramic membrane equipment with the aperture of 100-1000 nm to obtain refined filtrate;
s5: concentrating: concentrating the obtained fine filtration filtrate at the temperature of 50-60 ℃ and the vacuum degree of less than or equal to minus 0.1MPa to obtain a concentrated solution, wherein the concentration of the solid matters of the concentrated solution is 10-20%;
s6: spray drying: and adding the dissolution-assisting food auxiliary materials with the weight 1-3 times of the solid matters into the concentrated solution, uniformly stirring, and performing spray drying to obtain the Cordyceps militaris extract water-soluble powder.
2. The method for preparing water-soluble powder of Cordyceps militaris extract as claimed in claim 1, wherein in step S3, the number of heat extraction is 1-3, and the time for each extraction is 100-150 min.
3. The method for preparing a water-soluble powder of Cordyceps militaris extract as set forth in claim 1, wherein the step S4 comprises: centrifuging the obtained extract for 10-20 min at 2000-3000 r/min, taking out supernatant, and selecting supernatant for refining.
4. The method for preparing a water-soluble powder of Cordyceps militaris extract as claimed in claim 3, wherein the extract obtained by taking out the supernatant is fed back to the heat extraction apparatus of step S3 for the cyclic reflux heat extraction operation.
5. The method for preparing water-soluble powder of Cordyceps militaris extract as claimed in claim 1, wherein the fine filtrate obtained in the step S4 is concentrated in a vacuum concentrating apparatus.
6. The method for preparing water-soluble powder of Cordyceps militaris extract as claimed in claim 1, wherein the auxiliary materials of the dissolution-promoting food in step S6 comprise cyclodextrin and povidone.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117099612A (en) * | 2023-09-04 | 2023-11-24 | 西藏月王药诊生态藏药科技有限公司 | Cordyceps militaris cultivation and purification method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117099612A (en) * | 2023-09-04 | 2023-11-24 | 西藏月王药诊生态藏药科技有限公司 | Cordyceps militaris cultivation and purification method |
| CN117099612B (en) * | 2023-09-04 | 2024-03-26 | 西藏月王药诊生态藏药科技有限公司 | Cordyceps militaris cultivation and purification method |
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