CN110551586A - giant salamander protein peptide health wine and brewing method thereof - Google Patents

giant salamander protein peptide health wine and brewing method thereof Download PDF

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Publication number
CN110551586A
CN110551586A CN201910829586.5A CN201910829586A CN110551586A CN 110551586 A CN110551586 A CN 110551586A CN 201910829586 A CN201910829586 A CN 201910829586A CN 110551586 A CN110551586 A CN 110551586A
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parts
giant salamander
protein peptide
salamander protein
brewing
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付俊
吉建国
夏豫忠
杨斌
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Hubei Shi Yao Ji Tian Agricultural Development Co Ltd
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Hubei Shi Yao Ji Tian Agricultural Development Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/618Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/63Arthropods
    • A61K35/64Insects, e.g. bees, wasps or fleas
    • A61K35/644Beeswax; Propolis; Royal jelly; Honey
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481Astragalus (milkvetch)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/65Paeoniaceae (Peony family), e.g. Chinese peony
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/71Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/72Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A61K36/725Ziziphus, e.g. jujube
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/73Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/81Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815Lycium (desert-thorn)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/01Hydrolysed proteins; Derivatives thereof
    • A61K38/012Hydrolysed proteins; Derivatives thereof from animals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/20Hypnotics; Sedatives
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    • A61P25/00Drugs for disorders of the nervous system
    • A61P25/28Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/06Antihyperlipidemics
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    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
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    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00General protective or antinoxious agents
    • A61P39/02Antidotes
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P39/06Free radical scavengers or antioxidants
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P9/00Drugs for disorders of the cardiovascular system
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/021Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn
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    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/021Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn
    • C12G3/022Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn of botanical genus Oryza, e.g. rice
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
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    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
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    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/026Preparation of other alcoholic beverages by fermentation with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides, added before or during the fermentation stage; with flavouring ingredients added before or during the fermentation stage

Abstract

The invention discloses giant salamander protein peptide health wine which comprises the following raw material components in parts by weight: 10-20 parts of giant salamander protein peptide powder, 16-18 parts of moringa leaves, 13-17 parts of astragalus membranaceus, 10-15 parts of Chinese dates, 8-10 parts of Chinese wolfberry, 3-4 parts of oysters, 4-8 parts of white paeony roots, 1-2 parts of raspberries and 8-12 parts of honey. The health wine provided by the invention can soothe nerves, tonify qi, resist oxidation, enhance immunity, and has the effects of supplementing various nutrient substances, tonifying qi, nourishing yin, promoting the production of body fluid, relieving restlessness, promoting blood circulation, reducing blood pressure and blood fat and the like. The health-preserving wine provided by the invention has the advantages of reasonable formula, obvious curative effect, safety, reliability and convenience in eating; has good synergistic effect on tranquilization, anti-aging, immunity enhancement, dehumidification, detoxification, memory improvement and the like, has comprehensive functions, is suitable for various crowds, has unique curative effect on neurodegenerative diseases, insomnia, climacteric syndrome patients and people with low immunity, and is suitable for long-term administration.

Description

Giant salamander protein peptide health wine and brewing method thereof
Technical Field
The invention belongs to the technical field of health-care food, and particularly relates to giant salamander protein peptide health wine and a brewing method thereof.
background
Health preserving wine has a history of thousands of years and is an important component of Chinese medical science; almost no record exists in the past medical works of China that the medicinal liquor treats diseases and builds up health; with the progress of science and technology, modern means such as extraction, leaching and bioengineering are developed on the basis of the traditional process of traditional Chinese medicine wine soaking to extract effective components in traditional Chinese medicines to prepare high-content functional medicinal wine; however, the existing health-preserving wine has various varieties, poor effect, unobvious health-care effect and poor taste.
disclosure of Invention
in view of the above, the invention mainly aims to provide the giant salamander protein peptide health wine, which solves the problems of single component, unobvious health effect and poor taste of the existing health wine product; the invention also provides a brewing method of the giant salamander protein peptide health wine.
In view of the above, the invention mainly aims to provide the giant salamander protein peptide health wine, which solves the problems of unobvious health care effect and poor taste of the existing health wine; the invention also provides a brewing method of the giant salamander protein peptide health wine.
In order to achieve the purpose, the technical scheme of the invention is realized as follows: the giant salamander protein peptide health wine is characterized by comprising the following raw material components in parts by weight: 10-20 parts of giant salamander protein peptide powder, 16-18 parts of moringa leaves, 13-17 parts of astragalus membranaceus, 10-15 parts of Chinese dates, 8-10 parts of Chinese wolfberry, 3-4 parts of oysters, 4-8 parts of white paeony roots, 1-2 parts of raspberries and 8-12 parts of honey.
The other technical scheme of the invention is realized as follows: a brewing method of giant salamander protein peptide health wine is realized by the following steps:
step 1, respectively weighing the following components in parts by weight: 10-20 parts of giant salamander protein peptide powder, 16-18 parts of moringa oleifera leaves, 13-17 parts of astragalus membranaceus, 10-15 parts of Chinese dates, 8-10 parts of Chinese wolfberry, 3-4 parts of oysters, 4-8 parts of white paeony roots, 1-2 parts of raspberries and 8-12 parts of honey, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 15-20 hours to obtain a fermentation base material;
and 3, fermenting the fermentation base material obtained in the step 2 in a dark sealing mode at the temperature of 13-25 ℃, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
Preferably, in the step 2, the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is (0.01-0.1): 1; the mass ratio of the yeast to the grains for brewing is (0.005-0.02): 1.
Preferably, in the step 2, the grain for brewing wine is at least one of corn, rice, sorghum, barley, wheat and highland barley.
preferably, in the step 2, the mass of the fermentation base material is 2-3 times of that of the grains for brewing wine.
preferably, in the step 2, the step of preparing the fermentation base material comprises the following steps:
step 1, mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
step 2, placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing grains for brewing wine on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 60-90 min; and then adding the steamed mixture of the giant salamander protein peptide powder and grains for brewing into boiling yeast water, and boiling for 12-15 h to obtain a fermentation base material.
preferably, in the step 1, the giant salamander protein peptide powder is prepared by the following steps:
1) freeze-drying fresh giant salamander meat until the water content is less than 1%, and crushing the freeze-dried giant salamander meat to obtain giant salamander meat powder;
2) extracting the giant salamander meat powder obtained in the step 1 by using No. 6 solvent oil to remove fat, and volatilizing the solvent to obtain degreased giant salamander meat powder;
3) Mixing the degreased giant salamander meat powder obtained in the step 2 with a phosphoric acid buffer solution with the pH value of 6.5-7.5 in a proportion of 1 kg: uniformly mixing 2L-5L of solid-liquid ratio, and sieving by a 40-mesh sieve to obtain a mixed solution;
4) adding a complex enzyme into the mixed solution obtained in the step 3, and maintaining the temperature at 40-50 ℃ for enzymolysis for 1-3 h to obtain an enzymolysis solution;
5) heating the enzymolysis liquid obtained in the step 4 to 90-100 ℃, maintaining for 10-15 min, inactivating enzyme, sterilizing, centrifuging, and filtering to obtain giant salamander protein peptide liquid;
6) and (4) concentrating and drying the giant salamander polypeptide liquid obtained in the step (5) to obtain giant salamander protein peptide powder.
Preferably, in the step 2), the 6# solvent oil is petroleum ether or n-hexane.
preferably, in the step 4), the mass ratio of the addition amount of the complex enzyme to the defatted giant salamander meat powder in the mixed solution is 0.1%.
preferably, the compound enzyme is a compound enzyme of animal protease and neutral protease; the mass ratio of the compound enzyme to the animal protease to the neutral protease is 4: 3, complex enzyme.
Compared with the prior art, the health wine provided by the invention has reasonable formula, safety and effectiveness, and can be taken for a long time; in the preparation process, the raw materials are fermented to prepare the wine, and the nutritional effects of all the components are combined, so that the taste is improved to a greater extent, the effective components of the mixture can be well reserved, and the wine is more beneficial to taking and absorption by human bodies; the giant salamander protein peptide powder and other traditional Chinese medicine components are gradually dissolved in the health-preserving wine, so that the absorption and the effect of the giant salamander protein peptide powder are more favorably realized; the health wine disclosed by the invention can be used for soothing nerves, tonifying qi, resisting oxidation and enhancing immunity, and also has the effects of supplementing various nutrient substances, tonifying qi, nourishing yin, promoting the production of body fluid, relieving restlessness, promoting blood circulation, reducing blood pressure and blood fat and the like; the health-preserving wine provided by the invention has the advantages of reasonable formula, obvious curative effect, safety, reliability and convenience in eating; has good synergistic effect on tranquilization, anti-aging, immunity enhancement, dehumidification, detoxification, memory improvement and the like, has comprehensive functions, is suitable for various crowds, has unique curative effect on neurodegenerative diseases, insomnia, climacteric syndrome patients and people with low immunity, and is suitable for long-term administration.
Detailed Description
in order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The giant salamander protein peptide health wine provided by the embodiment of the invention comprises the following raw material components in parts by weight: 10-20 parts of giant salamander protein peptide powder, 16-18 parts of moringa leaves, 13-17 parts of astragalus membranaceus, 10-15 parts of Chinese dates, 8-10 parts of Chinese wolfberry, 3-4 parts of oysters, 4-8 parts of white paeony roots, 1-2 parts of raspberries and 8-12 parts of honey.
the invention provides a brewing method of giant salamander protein peptide health wine, which is realized by the following steps:
Step 1, respectively weighing the following components in parts by weight: 10-20 parts of giant salamander protein peptide powder, 16-18 parts of moringa oleifera leaves, 13-17 parts of astragalus membranaceus, 10-15 parts of Chinese dates, 8-10 parts of Chinese wolfberry, 3-4 parts of oysters, 4-8 parts of white paeony roots, 1-2 parts of raspberries and 8-12 parts of honey, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
the giant salamander protein peptide powder is prepared by the following steps:
1) freeze-drying fresh giant salamander meat until the water content is less than 1%, and crushing the freeze-dried giant salamander meat to obtain giant salamander meat powder;
2) Extracting the giant salamander meat powder obtained in the step 1 by using No. 6 solvent oil to remove fat, and volatilizing the solvent to obtain degreased giant salamander meat powder; wherein the No. 6 solvent oil is petroleum ether or n-hexane;
3) Mixing the degreased giant salamander meat powder obtained in the step 2 with a phosphoric acid buffer solution with the pH value of 6.5-7.5 in a proportion of 1 kg: uniformly mixing 2L-5L of solid-liquid ratio, and sieving by a 40-mesh sieve to obtain a mixed solution;
4) Adding a complex enzyme into the mixed solution obtained in the step 3, and carrying out enzymolysis for 1-3 hours at the temperature of 40-50 ℃ to obtain an enzymolysis solution; wherein the mass ratio of the addition amount of the complex enzyme to the mass ratio of the degreased giant salamander meat powder in the mixed solution is 0.1%; the complex enzyme is the complex enzyme of animal protease and neutral protease; the mass ratio of the compound enzyme to the animal protease to the neutral protease is 4: 3, complex enzyme;
5) heating the enzymolysis liquid obtained in the step 4 to 90-100 ℃, maintaining for 10-15 min, inactivating enzyme, sterilizing, centrifuging, and filtering to obtain giant salamander protein peptide liquid;
6) Concentrating and drying the giant salamander polypeptide liquid obtained in the step 5 to obtain giant salamander white polypeptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 15-20 hours to obtain a fermentation base material; wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is (0.01-0.1): 1; the mass ratio of the yeast to the grain for brewing is (0.005-0.02): 1; the grains for brewing wine are at least one of corn, rice, sorghum, barley, wheat and highland barley; the mass of the fermentation base material is 2-3 times of that of grains for brewing wine;
in addition, the fermentation base material is prepared by the following steps:
1) mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing grains for brewing wine on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 60-90 min; and then adding the steamed mixture of the giant salamander protein peptide powder and grains for brewing into boiling yeast water, and boiling for 12-15 h to obtain a fermentation base material.
And 3, fermenting the fermentation base material obtained in the step 2 in a dark sealing mode at the temperature of 13-25 ℃, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
The health wine provided by the invention has reasonable formula, is safe and effective, and can be taken for a long time; in the preparation process, the raw materials are fermented to prepare the wine, and the nutritional effects of all the components are combined, so that the taste is improved to a greater extent, the effective components of the mixture can be well reserved, and the wine is more beneficial to taking and absorption by human bodies; the giant salamander protein peptide powder and other traditional Chinese medicine components (such as radix scutellariae) are gradually dissolved in the health-preserving wine, so that the absorption and the effect of the giant salamander protein peptide powder are more favorably realized; the health wine provided by the invention can soothe nerves, tonify qi, resist oxidation and enhance immunity, and has the effects of supplementing various nutrient substances, tonifying qi, nourishing yin, promoting the production of body fluid, relieving restlessness, promoting blood circulation, reducing blood pressure and blood fat and the like. The health-preserving wine provided by the invention has the advantages of reasonable formula, obvious curative effect, safety, reliability and convenience in eating; has good synergistic effect on tranquilization, anti-aging, immunity enhancement, dehumidification, detoxification, memory improvement and the like, has comprehensive functions, is suitable for various crowds, has unique curative effect on neurodegenerative diseases, insomnia, climacteric syndrome patients and people with low immunity, and is suitable for long-term administration.
Example 1
The giant salamander protein peptide powder is prepared by the following method:
1) Freeze-drying fresh giant salamander meat until the water content is less than 1%, and crushing the freeze-dried giant salamander meat to obtain giant salamander meat powder;
2) extracting giant salamander meat powder with No. 6 solvent oil to remove fat, and volatilizing the solvent to obtain defatted giant salamander meat powder; wherein the main component of the No. 6 solvent oil is petroleum ether;
3) Mixing the degreased giant salamander meat powder with a phosphoric acid buffer solution with the pH value of 6.5-7.5 in a proportion of 1 kg: uniformly mixing 2L of solid-liquid ratio, and sieving with a 40-mesh sieve to obtain a mixed solution I;
4) adding a complex enzyme into the mixed solution I, and maintaining the temperature at 40 ℃ for enzymolysis for 1h to obtain an enzymolysis solution;
wherein the mass ratio of the addition amount of the complex enzyme to the degreased giant salamander meat powder in the mixed solution I is 0.1%; the mass ratio of the compound enzyme to the animal protease to the neutral protease is 4: 3, complex enzyme;
5) heating the enzymolysis liquid to 90 ℃, maintaining for 10min, inactivating enzyme, sterilizing, centrifuging, and filtering to obtain giant salamander protein peptide liquid;
6) and concentrating and drying the giant salamander polypeptide liquid to obtain giant salamander protein peptide powder.
example 2
the giant salamander protein peptide powder is prepared by the following method:
1) freeze-drying fresh giant salamander meat until the water content is less than 1%, and crushing the freeze-dried giant salamander meat to obtain giant salamander meat powder;
2) Extracting giant salamander meat powder with No. 6 solvent oil to remove fat, and volatilizing the solvent to obtain defatted giant salamander meat powder; wherein the 6# solvent oil mainly contains n-hexane;
3) mixing the degreased giant salamander meat powder with a phosphoric acid buffer solution with the pH value of 6.5-7.5 in a proportion of 1 kg: uniformly mixing 5L of solid-liquid ratio, and sieving with a 40-mesh sieve to obtain a mixed solution I;
4) adding a complex enzyme into the mixed solution I, and maintaining the temperature at 50 ℃ for enzymolysis for 3 hours to obtain an enzymolysis solution;
Wherein the mass ratio of the addition amount of the complex enzyme to the degreased giant salamander meat powder in the mixed solution I is 0.1%; the mass ratio of the compound enzyme to the animal protease to the neutral protease is 4: 3, complex enzyme;
5) heating the enzymolysis liquid to 100 ℃, maintaining for 15min, inactivating enzyme, sterilizing, centrifuging, and filtering to obtain giant salamander protein peptide liquid;
6) And concentrating and drying the giant salamander polypeptide liquid to obtain giant salamander protein peptide powder.
example 3
The giant salamander protein peptide powder is prepared by the following method:
1) freeze-drying fresh giant salamander meat until the water content is less than 1%, and crushing the freeze-dried giant salamander meat to obtain giant salamander meat powder;
2) extracting giant salamander meat powder with No. 6 solvent oil to remove fat, and volatilizing the solvent to obtain defatted giant salamander meat powder; wherein the 6# solvent oil mainly contains n-hexane;
3) Mixing the degreased giant salamander meat powder with a phosphoric acid buffer solution with the pH value of 6.5-7.5 in a proportion of 1 kg: uniformly mixing 4L of solid-liquid ratio, and sieving with a 40-mesh sieve to obtain a mixed solution I;
4) adding a complex enzyme into the mixed solution I, and maintaining the temperature at 45 ℃ for enzymolysis for 2 hours to obtain an enzymolysis solution; wherein the mass ratio of the addition amount of the complex enzyme to the degreased giant salamander meat powder in the mixed solution I is 0.1%; the mass ratio of the compound enzyme to the animal protease to the neutral protease is 4: 3, complex enzyme;
5) Heating the enzymolysis liquid to 95 ℃, maintaining for 13min, inactivating enzyme, sterilizing, centrifuging, and filtering to obtain giant salamander protein peptide liquid;
6) and concentrating and drying the giant salamander peptide liquid to obtain giant salamander protein peptide powder.
Example 4
the giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 18g of giant salamander protein peptide powder prepared in example 1, 17 parts of moringa leaves, 15 parts of astragalus membranaceus, 12 parts of Chinese date, 9 parts of medlar, 3 parts of oyster, 6 parts of white paeony root, 1 part of raspberry and 10 parts of honey.
the brewing method of the giant salamander protein peptide health wine is realized by the following steps:
step 1, respectively weighing the following components in parts by weight: 18 parts of giant salamander protein peptide powder, 17 parts of moringa leaves, 15 parts of astragalus membranaceus, 12 parts of Chinese dates, 9 parts of Chinese wolfberry, 3 parts of oysters, 6 parts of white paeony roots, 1 part of raspberries and 10 parts of honey prepared in example 1, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 18 hours to obtain a fermentation base material;
Wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.05: 1; the mass ratio of the yeast to the grains for brewing is 0.01: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 2 times of that of the grains for brewing wine;
in addition, the fermentation base material is prepared by the following steps:
1) Mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) Placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 75 min; then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 13 hours to obtain a fermentation base material;
and 3, hermetically fermenting the fermentation base material obtained in the step 2 at 20 ℃ in a dark place, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
example 5
The giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 10 parts of giant salamander protein peptide powder prepared in example 1, 18 parts of moringa leaves, 13 parts of astragalus membranaceus, 15 parts of Chinese date, 8 parts of medlar, 4 parts of oyster, 4 parts of white paeony root, 2 parts of raspberry and 8 parts of honey.
The brewing method of the giant salamander protein peptide health wine is realized by the following steps:
step 1, respectively weighing the following components in parts by weight: 10 parts of giant salamander protein peptide powder prepared in example 1, 18 parts of moringa oleifera leaves, 13 parts of astragalus membranaceus, 15 parts of Chinese date, 8 parts of Chinese wolfberry, 4 parts of oyster, 4 parts of white peony root, 2 parts of raspberry and 8 parts of honey, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 15 hours to obtain a fermentation base material;
Wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.01: 1; the mass ratio of the yeast to the grains for brewing is 0.005: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 2 times of that of the grains for brewing wine;
in addition, the fermentation base material is prepared by the following steps:
1) mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) Placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 60 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 15 hours to obtain a fermentation base material.
And 3, fermenting the fermentation base material obtained in the step 2 at 13 ℃ in a dark sealed manner, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
example 6
The giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 20 parts of giant salamander protein peptide powder prepared in example 1, 16 parts of moringa leaves, 17 parts of astragalus membranaceus, 10 parts of Chinese dates, 10 parts of Chinese wolfberry, 3 parts of oysters, 8 parts of white paeony roots, 1 part of raspberries and 12 parts of honey.
The brewing method of the giant salamander protein peptide health wine is realized by the following steps:
Step 1, respectively weighing the following components in parts by weight: 20 parts of giant salamander protein peptide powder, 16 parts of moringa leaves, 17 parts of astragalus membranaceus, 10 parts of Chinese dates, 10 parts of Chinese wolfberry, 3 parts of oysters, 8 parts of white paeony roots, 1 part of raspberries and 12 parts of honey prepared in example 1, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
Step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 20 hours to obtain a fermentation base material;
wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.1: 1; the mass ratio of the yeast to the grain for brewing is 0.02: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 3 times of that of the grains for brewing wine;
in addition, the fermentation base material is prepared by the following steps:
1) Mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 90 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 12 hours to obtain a fermentation base material.
And 3, fermenting the fermentation base material obtained in the step 2 at 25 ℃ in a dark sealed manner, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
Example 7
The giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 18g of giant salamander protein peptide powder prepared in example 2, 17 parts of moringa leaves, 15 parts of astragalus membranaceus, 12 parts of Chinese date, 9 parts of medlar, 3 parts of oyster, 6 parts of white paeony root, 1 part of raspberry and 10 parts of honey.
the brewing method of the giant salamander protein peptide health wine is realized by the following steps:
step 1, respectively weighing the following components in parts by weight: 18 parts of giant salamander protein peptide powder, 17 parts of moringa leaves, 15 parts of astragalus membranaceus, 12 parts of Chinese dates, 9 parts of Chinese wolfberry, 3 parts of oysters, 6 parts of white paeony roots, 1 part of raspberries and 10 parts of honey prepared in example 2, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 18 hours to obtain a fermentation base material;
Wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.05: 1; the mass ratio of the yeast to the grains for brewing is 0.01: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 2 times of that of the grains for brewing wine;
In addition, the fermentation base material is prepared by the following steps:
1) mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) Placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 75 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 13 hours to obtain a fermentation base material.
and 3, hermetically fermenting the fermentation base material obtained in the step 2 at 20 ℃ in a dark place, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
Example 8
the giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 10g of giant salamander protein peptide powder prepared in example 2, 18 parts of moringa leaves, 13 parts of astragalus membranaceus, 15 parts of Chinese date, 8 parts of medlar, 4 parts of oyster, 4 parts of white peony root, 2 parts of raspberry and 8 parts of honey.
the brewing method of the giant salamander protein peptide health wine is realized by the following steps:
step 1, respectively weighing the following components in parts by weight: 10 parts of giant salamander protein peptide powder, 18 parts of moringa leaves, 13 parts of astragalus membranaceus, 15 parts of Chinese dates, 8 parts of Chinese wolfberry, 4 parts of oysters, 4 parts of white paeony roots, 2 parts of raspberries and 8 parts of honey prepared in example 2, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 15 hours to obtain a fermentation base material;
wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.01: 1; the mass ratio of the yeast to the grains for brewing is 0.005: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 2 times of that of the grains for brewing wine;
In addition, the fermentation base material is prepared by the following steps:
1) Mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) Placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 60 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 15 hours to obtain a fermentation base material.
And 3, fermenting the fermentation base material obtained in the step 2 at 13 ℃ in a dark sealed manner, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
Example 9
The giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 20 parts of giant salamander protein peptide powder prepared in example 2, 16 parts of moringa leaves, 17 parts of astragalus membranaceus, 10 parts of Chinese dates, 10 parts of Chinese wolfberry, 3 parts of oysters, 8 parts of white paeony roots, 1 part of raspberries and 12 parts of honey.
The brewing method of the giant salamander protein peptide health wine is realized by the following steps:
Step 1, respectively weighing the following components in parts by weight: 20 parts of giant salamander protein peptide powder, 16 parts of moringa leaves, 17 parts of astragalus membranaceus, 10 parts of Chinese dates, 10 parts of Chinese wolfberry, 3 parts of oysters, 8 parts of white paeony roots, 1 part of raspberries and 12 parts of honey prepared in example 2, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
Step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 20 hours to obtain a fermentation base material;
Wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.1: 1; the mass ratio of the yeast to the grain for brewing is 0.02: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 3 times of that of the grains for brewing wine;
in addition, the fermentation base material is prepared by the following steps:
1) Mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) Placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 90 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 12 hours to obtain a fermentation base material.
and 3, fermenting the fermentation base material obtained in the step 2 at 25 ℃ in a dark sealed manner, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
Example 10
The giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 18g of giant salamander protein peptide powder prepared in example 3, 17 parts of moringa leaves, 15 parts of astragalus membranaceus, 12 parts of Chinese date, 9 parts of medlar, 3 parts of oyster, 6 parts of white paeony root, 1 part of raspberry and 10 parts of honey.
The brewing method of the giant salamander protein peptide health wine is realized by the following steps:
step 1, respectively weighing the following components in parts by weight: 18 parts of giant salamander protein peptide powder, 17 parts of moringa leaves, 15 parts of astragalus membranaceus, 12 parts of Chinese dates, 9 parts of Chinese wolfberry, 3 parts of oysters, 6 parts of white paeony roots, 1 part of raspberries and 10 parts of honey prepared in example 3, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 18 hours to obtain a fermentation base material;
Wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.05: 1; the mass ratio of the yeast to the grains for brewing is 0.01: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 2 times of that of the grains for brewing wine;
In addition, the fermentation base material is prepared by the following steps:
1) Mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 75 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 13 hours to obtain a fermentation base material.
and 3, hermetically fermenting the fermentation base material obtained in the step 2 at 20 ℃ in a dark place, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
example 11
the giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 10 parts of giant salamander protein peptide powder prepared in example 3, 18 parts of moringa leaves, 13 parts of astragalus membranaceus, 15 parts of Chinese date, 8 parts of medlar, 4 parts of oyster, 4 parts of white peony root, 2 parts of raspberry and 8 parts of honey.
the brewing method of the giant salamander protein peptide health wine is realized by the following steps:
Step 1, respectively weighing the following components in parts by weight: 10 parts of giant salamander protein peptide powder, 18 parts of moringa leaves, 13 parts of astragalus membranaceus, 15 parts of Chinese dates, 8 parts of Chinese wolfberry, 4 parts of oysters, 4 parts of white paeony roots, 2 parts of raspberries and 8 parts of honey prepared in example 3, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 15 hours to obtain a fermentation base material;
Wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.01: 1; the mass ratio of the yeast to the grains for brewing is 0.005: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 2 times of that of the grains for brewing wine;
In addition, the fermentation base material is prepared by the following steps:
1) Mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 60 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 15 hours to obtain a fermentation base material.
And 3, fermenting the fermentation base material obtained in the step 2 at 13 ℃ in a dark sealed manner, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
Example 12
the giant salamander protein peptide health wine comprises the following raw material components in parts by weight: 20 parts of giant salamander protein peptide powder prepared in example 3, 16 parts of moringa leaves, 17 parts of astragalus membranaceus, 10 parts of Chinese dates, 10 parts of Chinese wolfberry, 3 parts of oysters, 8 parts of white paeony roots, 1 part of raspberries and 12 parts of honey.
The brewing method of the giant salamander protein peptide health wine is realized by the following steps:
step 1, respectively weighing the following components in parts by weight: 20 parts of giant salamander protein peptide powder, 16 parts of moringa leaves, 17 parts of astragalus membranaceus, 10 parts of Chinese dates, 10 parts of Chinese wolfberry, 3 parts of oysters, 8 parts of white paeony roots, 1 part of raspberries and 12 parts of honey prepared in example 3, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 20 hours to obtain a fermentation base material;
Wherein the mass ratio of the mixture of the giant salamander protein peptide powder to the grains for brewing is 0.1: 1; the mass ratio of the yeast to the grain for brewing is 0.02: 1; the grains for brewing wine are corn; the mass of the fermentation base material is 3 times of that of the grains for brewing wine;
In addition, the fermentation base material is prepared by the following steps:
1) mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
2) placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing the corn on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 90 min; and then adding the mixture of the steamed giant salamander protein peptide powder and the corn into boiling yeast water, and boiling for 12 hours to obtain a fermentation base material.
and 3, fermenting the fermentation base material obtained in the step 2 at 25 ℃ in a dark sealed manner, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
comparative example
The raw material components in the health wine of this comparative example do not include giant salamander protein peptide powder, and the rest are the same as those in example 4, and therefore, the preparation process is not described in detail herein.
the basic research results of the giant salamander protein peptide health wine, such as the functional experiment, are as follows:
The giant salamander protein peptide health wine obtained in the above examples 4, 7 and 10 and comparative example was used for the following clinical treatments:
first, treat the object
400 cases of the following people aged 18-50 years are selected: 100 cases of lassitude, amnesia, cold body and limbs; insomnia, neurasthenia, weakness of waist and knees, alopecia, odontoseisis, short breath, debilitation, etc. 100 cases; 100 cases of climacteric syndrome, endocrine disturbance, skin acne, and speckle; 100 cases of patients with gastrointestinal discomfort and deficiency-cold of spleen and stomach.
Randomly dividing into 200 cases of treatment group and control group, wherein 50 cases of lassitude, amnesia, cold-form and cold-limbs; insomnia, neurasthenia, asthenia of waist and knees, alopecia, odontoseisis, short breath, debilitation, etc. in 50 cases; 50 cases of climacteric syndrome, endocrine disturbance, skin acne, and speckle; 50 cases of the patients with gastrointestinal discomfort and deficiency-cold of spleen and stomach.
Second, the administration method
The treatment components are three groups, wherein the first group is orally taken with the health-preserving wine of the embodiment 4, the second group is orally taken with the health-preserving wine of the embodiment 7, and the third group is orally taken with the health-preserving wine of the embodiment 10, the health-preserving wine is drunk 3 times a day (once in the morning, at noon and at night), 50-80 mL is drunk each time, the oral administration is carried out for 21 days continuously, and the results are observed and recorded.
The patients of the control group took the health preserving wine of the comparative example orally for 21 days, and observed and recorded the results.
Three, result in
The giant salamander protein peptide health wine provided by the invention has the following clinical treatment effects: the effective rate for lassitude, amnesia, cold in shape and limbs reaches 97.5 percent; the effective rate for insomnia, neurasthenia, weakness of waist and knees, alopecia, tooth shake, short breath, hypodynamia and other symptoms reaches 98.2 percent; the effective rate for people with climacteric syndrome, endocrine dyscrasia, skin acne and skin spots reaches 97.9 percent; the effective rate for patients with gastrointestinal discomfort and deficiency-cold of spleen and stomach reaches 94.3 percent; and in the patients with cold limbs, short breath, debilitation, climacteric syndrome, endocrine disturbance, skin acne and macula, the symptoms disappear after taking for 10 days, and the dosage is reduced or stopped.
the effective rate of the control group on lassitude, amnesia, cold and cold limbs reaches 36.2 percent; the effective rate for insomnia, neurasthenia, weakness of waist and knees, odontoseisis, short breath and hypodynamia and other symptoms reaches 35.7 percent; the effective rate for patients with diabetes, hypertension, hyperlipidemia and coronary heart disease reaches 45.6.9%; the effective rate for people with climacteric syndrome, endocrine dyscrasia, skin acne and skin spots reaches 51.6 percent; the effective rate for patients with gastrointestinal discomfort and deficiency-cold of spleen and stomach reaches 67.2 percent.
In conclusion, the health-care wine provided by the invention has obvious curative effects on patients with lassitude, amnesia, cold limbs, insomnia and sleeplessness, neurasthenia, weakness of waist and knees, alopecia and tooth shake, short breath and hypodynamia, diabetes, hypertension, hyperlipidemia, coronary heart disease, climacteric syndrome, endocrine dyscrasia, skin acne, freckle, gastrointestinal discomfort, deficiency-cold spleen and stomach and the like, and has better effects than those of a control group.
the giant salamander protein peptide health wine has the advantages of definite curative effect, safety in taking, no toxic or side effect and the like, and the prepared product has stable quality and is convenient to use; and the functions are comprehensive, and the health-care tea is suitable for various crowds and is suitable for long-term administration.
the above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (10)

1. the giant salamander protein peptide health wine is characterized by comprising the following raw material components in parts by weight: 10-20 parts of giant salamander protein peptide powder, 16-18 parts of moringa leaves, 13-17 parts of astragalus membranaceus, 10-15 parts of Chinese dates, 8-10 parts of Chinese wolfberry, 3-4 parts of oysters, 4-8 parts of white paeony roots, 1-2 parts of raspberries and 8-12 parts of honey.
2. a brewing method of giant salamander protein peptide health wine is characterized by comprising the following steps:
step 1, respectively weighing the following components in parts by weight: 10-20 parts of giant salamander protein peptide powder, 16-18 parts of moringa oleifera leaves, 13-17 parts of astragalus membranaceus, 10-15 parts of Chinese dates, 8-10 parts of Chinese wolfberry, 3-4 parts of oysters, 4-8 parts of white paeony roots, 1-2 parts of raspberries and 8-12 parts of honey, and uniformly mixing to obtain a mixture containing the giant salamander protein peptide powder;
Step 2, adding the mixture containing the giant salamander protein peptide powder obtained in the step 1, grains for brewing and yeast into water, and heating and cooking for 15-20 hours to obtain a fermentation base material;
and 3, fermenting the fermentation base material obtained in the step 2 in a dark sealing mode at the temperature of 13-25 ℃, and filtering after the fermentation is finished to obtain the giant salamander protein peptide health wine.
3. the brewing method of the giant salamander protein peptide health wine according to claim 2, wherein in the step 2, the mass ratio of the mixture of the giant salamander protein peptide powder to the grain for brewing is (0.01-0.1): 1; the mass ratio of the yeast to the grains for brewing is (0.005-0.02): 1.
4. the brewing method of the giant salamander protein peptide health wine according to claim 2, wherein in the step 2, the brewing grain is at least one of corn, rice, sorghum, barley, wheat and highland barley.
5. The brewing method of the giant salamander protein peptide health wine according to claim 2, wherein in the step 2, the mass of the fermentation base material is 2-3 times of that of brewing grains.
6. the brewing method of the giant salamander protein peptide health wine according to claim 5, wherein in the step 2, the step of preparing the fermentation base material comprises the following steps:
Step 1, mixing and dissolving yeast and water to obtain yeast water, and then putting the yeast water into a steamer;
Step 2, placing the steamer tray in a steamer and higher than the water surface of yeast water, flatly laying the mixture of the giant salamander protein peptide powder on the steamer tray, placing grains for brewing wine on the mixture of the giant salamander protein peptide powder, heating to boil, and keeping the boiled steam for steaming for 60-90 min; and then adding the steamed mixture of the giant salamander protein peptide powder and grains for brewing into boiling yeast water, and boiling for 12-15 h to obtain a fermentation base material.
7. the brewing method of the giant salamander protein peptide health wine according to claim 2, wherein in the step 1, the giant salamander protein peptide powder is prepared by the following steps:
1) Freeze-drying fresh giant salamander meat until the water content is less than 1%, and crushing the freeze-dried giant salamander meat to obtain giant salamander meat powder;
2) extracting the giant salamander meat powder obtained in the step 1 by using No. 6 solvent oil to remove fat, and volatilizing the solvent to obtain degreased giant salamander meat powder;
3) Mixing the degreased giant salamander meat powder obtained in the step 2 with a phosphoric acid buffer solution with the pH value of 6.5-7.5 in a proportion of 1 kg: uniformly mixing 2L-5L of solid-liquid ratio, and sieving by a 40-mesh sieve to obtain a mixed solution;
4) adding a complex enzyme into the mixed solution obtained in the step 3, and maintaining the temperature at 40-50 ℃ for enzymolysis for 1-3 h to obtain an enzymolysis solution;
5) heating the enzymolysis liquid obtained in the step 4 to 90-100 ℃, maintaining for 10-15 min, inactivating enzyme, sterilizing, centrifuging, and filtering to obtain giant salamander protein peptide liquid;
6) and (4) concentrating and drying the giant salamander polypeptide liquid obtained in the step (5) to obtain giant salamander protein peptide powder.
8. The brewing method of the giant salamander protein peptide health wine according to claim 7, wherein in the step 2), the 6# solvent oil is petroleum ether or n-hexane.
9. The brewing method of the giant salamander protein peptide health wine according to claim 7, wherein in the step 4), the mass ratio of the addition amount of the complex enzyme to the defatted giant salamander meat meal in the mixed solution is 0.1%.
10. the brewing method of the giant salamander protein peptide health wine according to claim 9, wherein the complex enzyme is a complex enzyme of animal protease and neutral protease; the mass ratio of the compound enzyme to the animal protease to the neutral protease is 4: 3, complex enzyme.
CN201910829586.5A 2019-09-03 2019-09-03 giant salamander protein peptide health wine and brewing method thereof Pending CN110551586A (en)

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CN110882279A (en) * 2019-12-11 2020-03-17 付俊 Application of giant salamander extract in medicine for treating chronic renal failure of diabetes
CN110882380A (en) * 2019-12-11 2020-03-17 付俊 Liver-protecting liquor additive and production method thereof

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* Cited by examiner, † Cited by third party
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CN110882279A (en) * 2019-12-11 2020-03-17 付俊 Application of giant salamander extract in medicine for treating chronic renal failure of diabetes
CN110882380A (en) * 2019-12-11 2020-03-17 付俊 Liver-protecting liquor additive and production method thereof

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